FvH4_5g17820

3,4-dihydroxy-2-butanone kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012302
Physical Location & Seq
Reverse (-)
10205892 .. 10212904
7013 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17820.t1

Sequence Viewer

Length: 1782 bp
ATGGCTTTCCAGGACAAGAAGCTCATCAACAACCCCAACGATGTGGTGACTGAGTTCATTGAAGGTCTGGTTGAGACTTACCCTGGGCTGCAGTACTTGGATGGTTTCCCTCAGGTGAAAGTTGTGTTGCGCGCTGATGTTTCCGGTGGTTCTTATGACAAAGTTGCAGTTATATCAGGAGGTGGGAGTGGGCATGAACCCGCACATGCTGGATTTGTGGGGGAAGGGATGTTGACAGCAGCTATCTGCGGGGATGTCTTTGCCTCTCCACCGGTTGATTCAATTCTAGCGGGCATCCGAGCTGTAACTGGTCCAATGGGTTGTCTCCTGATTGTCAAGAATTATACTGGGGACCGTCTGAATTTCGGTTTGGCTGCAGAGCAAGCAAAATCGGAAGGTTACAACGTAGAGACTGTAATTGTTGGGGATGATTGTGCATTGCCCCCGCCTCGAGGAATAGCTGGTCGGAGGGGTTTGGCCGGTACCATTCTTGTTCATAAGGTTGCTGGAGCTGCAGCTGCTTCTGGGAGTTCTCTTGCTGATGTTGCAGCAGAAGCAAGACGTGCATCAGAAATGGTTGGAACAATGGGTGTTGCATTATCTGTTTGCACACTGCCTGGTCGGGTCACACCAGACCGTTTGGGACCTGGAAAGATGGAACTTGGTCTTGGAATTCATGGAGAACCGGGTGCTGCTGTTGCTGACCTTCAGCCTGTGGATGTTATAGTCTCTCATGTTCTCAAGCAGATATTGTGTCCGGAGACTAATTATGTTCCGATTACTCGTGGAAGTAGAGTGGTGCTTATGGTCAATGGGTTAGGTGCAACTCCCGCAATGGAACTGATGATCACATCTGGTAAAGCAGTACCTAAGTTGCAGCTGGAACATGGATTAGCTGTTGATAGAGTTTATACCGGATCATTCATGACCTCTCTTGATATGGCAGGTTTTTCGATATCTATCATGAAGGCTGATCAAGCTATTTTACAACGTCTGGATGCTCCAACAAAGGCTCCCTATTGGCCTGTTGGCGTAGATGGCAATCACCCACCCGCAAAGATTCCTGTTCCATTGCCTCCTTCTCGTTCAACAAAGATCGATGAGCCATTAGGTCGACCAGCACAACTGAATGAACAAGGCCGTATTCTTGAGGTGGCAATTGAAGCAGCTGCAAATGCAGTTCAGAACCTCAGGGATGAATTGAATGATTGGGATAGCAAAGTAGGTGATGGTGATTGCGGGTCAACAATGTATAGAGGTGCAACAGCAATTTTGGAAGACATGCAGAATTATCCTCTGAATGATGCAGCGGAAACAGTGAACGAAATTGGTGCATCTGTTAGAAGAGTTATGGGAGGCACAAGTGGGATCCTGTATACCATATTTTGTAAGGCAGCGTATGCACAGTTGAAAACAAGCACCCAATCTGTTGTGACATCAAAAAATTGGGCTGAAGCACTTGAAGCTTCCATTGCTGCAGTCAGCAAATATGGTGGGGCTAGTGCTGGGTATCGCACATTGTTAGACGCCCTTATTCCAGCATCAGCAGTTCTTCGAGAGAGGTTAAATGCTGGTGATGATCCTGCAACTGCTTTTGTTCTGTCATCTGAAGCTGCAGTAGCTGGAGCTGAGTCAACTAAGCACATGCAGGCACAGGCTGGGCGTTCGAGCTATGTCTCTGGAGAGATACTAGCATCAGTCCCAGACCCAGGTGCAATGGCTGCAGCGTCATGGTACAAGGCAGCAGCCTTAGCTGTGAAGGAAAAATATCTGGCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00561 ko00680 ko01100 ko01120 ko01200 ko04622 map00051 map00561 map00680 map01100 map01120 map01200 map04622
Pfam Domains
Protein Families
Dak1 Dak2

Protein Analysis

594

Amino Acids

61.58

Weight (kDa)

5.3

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dak1 PF02733 22 - 339 5.2e-114 Dak1 domain
Dak2 PF02734 407 - 582 2.4e-41 DAK2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012302)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48430 AT1G48430 AT3G17770
fragaria_vesca FvH4_5g17820
malus_domestica MD06G1234200.v1.1 MD14G1240900.v1.1
prunus_persica Prupe.5G238700_v2.0.a1
pyrus_communis pycom06g20890 pycom14g20240
rosa_chinensis RchiOBHm_Chr7g0177761
rosa_laevigata RLG00000005506
rosa_multiflora Rmu_sc0006214.1_g000015
rosa_roxburghii Rroxscaffold_3G00275580
rosa_rugosa Rorug06G0409300
rosa_samantha Rh7AG008900 Rh7BG009000 Rh7CG009200 Rh7DG009000
rosa_wichuraiana Rw7G000730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 935
Acc65I GGTACC 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 482
AccI GTMKAC 2 cut(s) 1114, 1376
AccII CGCG 1 cut(s) 132
AccIII TCCGGA 1 cut(s) 757
AciI CCGC 8 cut(s) 201, 249, 290, 446, 831, 1053, 1239, 1310
AclWI GGATC 4 cut(s) 925, 1363, 1376, 1574
AcoI YGGCCR 1 cut(s) 477
AcsI RAATTY 2 cut(s) 361, 672
AcuI CTGAAG 3 cut(s) 692, 1473, 1629
AcyI GRCGYC 1 cut(s) 1527
AfaI GTAC 4 cut(s) 95, 484, 867, 1736
AfiI CCNNNNNNNGG 2 cut(s) 452, 1709
AgeI ACCGGT 1 cut(s) 271
AgsI TTSAA 7 cut(s) 62, 282, 1089, 1163, 1204, 1411, 1463
AjiI CACGTC 1 cut(s) 563
AjnI CCWGG 5 cut(s) 9, 82, 616, 646, 1708
AjuI GAANNNNNNNTTGG 4 cut(s) 353, 385, 651, 683
Alw26I GTCTC 6 cut(s) 68, 329, 404, 733, 755, 1681
AlwI GGATC 4 cut(s) 925, 1363, 1376, 1574
AlwNI CAGNNNCTG 1 cut(s) 1622
Ama87I CYCGRG 1 cut(s) 450
Aor13HI TCCGGA 1 cut(s) 757
AoxI GGCC 3 cut(s) 477, 1022, 1138
ApoI RAATTY 2 cut(s) 361, 672
AsiGI ACCGGT 1 cut(s) 271
Asp718I GGTACC 1 cut(s) 482
AspLEI GCGC 2 cut(s) 132, 134
AspS9I GGNCC 3 cut(s) 311, 352, 644
AsuC2I CCSGG 1 cut(s) 687
AsuHPI GGTGA 6 cut(s) 58, 127, 1037, 1238, 1244, 1586
AvaI CYCGRG 1 cut(s) 450
AvaII GGWCC 3 cut(s) 311, 352, 644
AxyI CCTNAGG 2 cut(s) 111, 1190
BamHI GGATCC 1 cut(s) 1368
BanI GGYRCC 1 cut(s) 482
BauI CACGAG 1 cut(s) 783
BbsI GAAGAC 1 cut(s) 1284
BccI CCATC 4 cut(s) 95, 649, 1031, 1223
BceAI ACGGC 1 cut(s) 1125
BcgI CGANNNNNNTGC 4 cut(s) 933, 967, 1313, 1347
BciT130I CCWGG 5 cut(s) 11, 84, 618, 648, 1710
BclI TGATCA 2 cut(s) 846, 973
BcnI CCSGG 1 cut(s) 687
BcoDI GTCTC 6 cut(s) 68, 329, 404, 733, 755, 1681
BfaI CTAG 3 cut(s) 287, 1500, 1691
BfmI CTRYAG 6 cut(s) 89, 375, 513, 1476, 1614, 1722
BfuAI ACCTGC 1 cut(s) 935
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 6 cut(s) 11, 84, 618, 648, 687, 1710
Bme18I GGWCC 3 cut(s) 311, 352, 644
BmeT110I CYCGRG 1 cut(s) 450
BmgBI CACGTC 1 cut(s) 563
BmgT120I GGNCC 3 cut(s) 311, 352, 644
BmiI GGNNCC 6 cut(s) 353, 484, 645, 1014, 1370, 1776
BmrFI CCNGG 6 cut(s) 11, 84, 618, 648, 687, 1710
BmrI ACTGGG 1 cut(s) 357
BmsI GCATC 7 cut(s) 303, 575, 988, 1294, 1343, 1550, 1703
BmuI ACTGGG 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 1284
BpmI CTGGAG 3 cut(s) 528, 1644, 1701
Bpu10I CCTNAGC 1 cut(s) 1750
BpuEI CTTGAG 2 cut(s) 725, 1169
BpuMI CCSGG 1 cut(s) 687
Bsa29I ATCGAT 1 cut(s) 1098
BsaBI GATNNNNATC 2 cut(s) 959, 1041
BsaHI GRCGYC 1 cut(s) 1527
BsaJI CCNNGG 3 cut(s) 82, 83, 1708
BsaWI WCCGGW 4 cut(s) 143, 271, 757, 914
BsaXI ACNNNNNCTCC 2 cut(s) 997, 1027
Bsc4I CCNNNNNNNGG 2 cut(s) 452, 1709
Bse118I RCCGGY 2 cut(s) 271, 479
Bse1I ACTGG 2 cut(s) 313, 352
Bse21I CCTNAGG 2 cut(s) 111, 1190
Bse3DI GCAATG 5 cut(s) 437, 840, 1070, 1470, 1722
Bse8I GATNNNNATC 2 cut(s) 959, 1041
BseAI TCCGGA 1 cut(s) 757
BseBI CCWGG 5 cut(s) 11, 84, 618, 648, 1710
BseCI ATCGAT 1 cut(s) 1098
BseDI CCNNGG 3 cut(s) 82, 83, 1708
BseGI GGATG 8 cut(s) 106, 234, 259, 294, 433, 724, 1003, 1201
BseJI GATNNNNATC 2 cut(s) 959, 1041
BseLI CCNNNNNNNGG 2 cut(s) 452, 1709
BseMI GCAATG 5 cut(s) 437, 840, 1070, 1470, 1722
BseMII CTCAG 4 cut(s) 42, 125, 1204, 1620
BseNI ACTGG 2 cut(s) 313, 352
BsePI GCGCGC 1 cut(s) 130
BseYI CCCAGC 2 cut(s) 1505, 1658
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 479, 1024, 1140
BshNI GGYRCC 1 cut(s) 482
BshTI ACCGGT 1 cut(s) 271
BshVI ATCGAT 1 cut(s) 1098
BsiHKCI CYCGRG 1 cut(s) 450
BsiSI CCGG 6 cut(s) 144, 272, 480, 686, 758, 915
BslFI GGGAC 3 cut(s) 365, 657, 1685
BslI CCNNNNNNNGG 2 cut(s) 452, 1709
BsmAI GTCTC 6 cut(s) 68, 329, 404, 733, 755, 1681
BsmFI GGGAC 3 cut(s) 365, 657, 1685
BsnI GGCC 3 cut(s) 479, 1024, 1140
BsoBI CYCGRG 1 cut(s) 450
Bsp13I TCCGGA 1 cut(s) 757
Bsp143I GATC 6 cut(s) 846, 917, 973, 1095, 1368, 1579
BspACI CCGC 8 cut(s) 201, 249, 290, 446, 831, 1053, 1239, 1310
BspANI GGCC 3 cut(s) 479, 1024, 1140
BspCNI CTCAG 4 cut(s) 43, 124, 1203, 1621
BspDI ATCGAT 1 cut(s) 1098
BspEI TCCGGA 1 cut(s) 757
BspFNI CGCG 1 cut(s) 132
BspHI TCATGA 2 cut(s) 924, 963
BspLI GGNNCC 6 cut(s) 353, 484, 645, 1014, 1370, 1776
BspMAI CTGCAG 6 cut(s) 93, 379, 517, 1480, 1618, 1726
BspMI ACCTGC 1 cut(s) 935
BspPI GGATC 4 cut(s) 925, 1363, 1376, 1574
BspT107I GGYRCC 1 cut(s) 482
BsrDI GCAATG 5 cut(s) 437, 840, 1070, 1470, 1722
BsrFI RCCGGY 2 cut(s) 271, 479
BsrI ACTGG 2 cut(s) 313, 352
BssAI RCCGGY 2 cut(s) 271, 479
BssECI CCNNGG 3 cut(s) 82, 83, 1708
BssHII GCGCGC 1 cut(s) 130
BssMI GATC 6 cut(s) 846, 917, 973, 1095, 1368, 1579
BssNAI GTATAC 1 cut(s) 1377
BssNI GRCGYC 1 cut(s) 1527
BssSI CACGAG 1 cut(s) 783
Bst1107I GTATAC 1 cut(s) 1377
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 5 cut(s) 11, 84, 618, 648, 1710
Bst4CI ACNGT 5 cut(s) 356, 415, 638, 1318, 1407
Bst6I CTCTTC 1 cut(s) 1339
BstACI GRCGYC 1 cut(s) 1527
BstAPI GCANNNNNTGC 3 cut(s) 563, 1400, 1721
BstC8I GCNNGC 4 cut(s) 132, 292, 384, 1650
BstDEI CTNAG 7 cut(s) 51, 111, 870, 1190, 1629, 1638, 1750
BstF5I GGATG 8 cut(s) 106, 234, 259, 294, 433, 724, 1003, 1201
BstFNI CGCG 1 cut(s) 132
BstHHI GCGC 2 cut(s) 132, 134
BstKTI GATC 6 cut(s) 849, 920, 976, 1098, 1371, 1582
BstMAI GTCTC 6 cut(s) 68, 329, 404, 733, 755, 1681
BstMBI GATC 6 cut(s) 846, 917, 973, 1095, 1368, 1579
BstNI CCWGG 5 cut(s) 11, 84, 618, 648, 1710
BstNSI RCATGY 3 cut(s) 209, 1285, 1648
BstSCI CCNGG 6 cut(s) 9, 82, 616, 646, 685, 1708
BstSFI CTRYAG 6 cut(s) 89, 375, 513, 1476, 1614, 1722
BstUI CGCG 1 cut(s) 132
BstV2I GAAGAC 1 cut(s) 1284
BstX2I RGATCY 1 cut(s) 1368
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 1368
BstZ17I GTATAC 1 cut(s) 1377
Bsu15I ATCGAT 1 cut(s) 1098
Bsu36I CCTNAGG 2 cut(s) 111, 1190
BsuRI GGCC 3 cut(s) 479, 1024, 1140
BsuTUI ATCGAT 1 cut(s) 1098
BtrI CACGTC 1 cut(s) 563
BtsCI GGATG 8 cut(s) 106, 234, 259, 294, 433, 724, 1003, 1201
BtsI GCAGTG 1 cut(s) 611
BtsIMutI CAGTG 2 cut(s) 611, 1323
BveI ACCTGC 1 cut(s) 935
Cac8I GCNNGC 4 cut(s) 132, 292, 384, 1650
CaiI CAGNNNCTG 1 cut(s) 1622
CciI TCATGA 2 cut(s) 924, 963
CfoI GCGC 2 cut(s) 132, 134
Cfr10I RCCGGY 2 cut(s) 271, 479
Cfr13I GGNCC 3 cut(s) 311, 352, 644
ClaI ATCGAT 1 cut(s) 1098
CseI GACGC 2 cut(s) 1535, 1716
Csp6I GTAC 4 cut(s) 94, 483, 866, 1735
CspAI ACCGGT 1 cut(s) 271
CspCI CAANNNNNGTGG 2 cut(s) 1474, 1509
CviQI GTAC 4 cut(s) 94, 483, 866, 1735
DdeI CTNAG 7 cut(s) 51, 111, 870, 1190, 1629, 1638, 1750
DpnI GATC 6 cut(s) 848, 919, 975, 1097, 1370, 1581
DpnII GATC 6 cut(s) 846, 917, 973, 1095, 1368, 1579
EaeI YGGCCR 1 cut(s) 477
Eam1104I CTCTTC 1 cut(s) 1339
EarI CTCTTC 1 cut(s) 1339
Eco32I GATATC 1 cut(s) 957
Eco47I GGWCC 3 cut(s) 311, 352, 644
Eco57I CTGAAG 3 cut(s) 692, 1473, 1629
Eco81I CCTNAGG 2 cut(s) 111, 1190
Eco88I CYCGRG 1 cut(s) 450
EcoO109I RGGNCCY 1 cut(s) 644
EcoRI GAATTC 1 cut(s) 672
EcoRII CCWGG 5 cut(s) 9, 82, 616, 646, 1708
EcoRV GATATC 1 cut(s) 957
FaqI GGGAC 3 cut(s) 365, 657, 1685
FauI CCCGC 7 cut(s) 208, 242, 283, 453, 838, 1060, 1232
FbaI TGATCA 2 cut(s) 846, 973
FblI GTMKAC 2 cut(s) 1114, 1376
FokI GGATG 8 cut(s) 113, 241, 266, 281, 440, 731, 1010, 1208
FspBI CTAG 3 cut(s) 287, 1500, 1691
GlaI GCGC 2 cut(s) 131, 133
GsaI CCCAGC 2 cut(s) 1509, 1662
GsuI CTGGAG 3 cut(s) 528, 1644, 1701
HaeIII GGCC 3 cut(s) 479, 1024, 1140
HapII CCGG 6 cut(s) 144, 272, 480, 686, 758, 915
HgaI GACGC 2 cut(s) 1535, 1716
HhaI GCGC 2 cut(s) 132, 134
Hin1I GRCGYC 1 cut(s) 1527
Hin6I GCGC 2 cut(s) 130, 132
HinP1I GCGC 2 cut(s) 130, 132
HincII GTYRAC 4 cut(s) 234, 1115, 1245, 1635
HindII GTYRAC 4 cut(s) 234, 1115, 1245, 1635
HindIII AAGCTT 1 cut(s) 1464
HinfI GANTC 3 cut(s) 278, 1060, 1631
HpaII CCGG 6 cut(s) 144, 272, 480, 686, 758, 915
HphI GGTGA 6 cut(s) 58, 127, 1037, 1238, 1244, 1586
Hpy166II GTNNAC 6 cut(s) 234, 1115, 1245, 1321, 1377, 1635
Hpy188I TCNGA 9 cut(s) 299, 360, 394, 468, 571, 777, 1185, 1299, 1609
Hpy8I GTNNAC 6 cut(s) 234, 1115, 1245, 1321, 1377, 1635
HpyAV CCTTC 7 cut(s) 56, 218, 389, 716, 961, 1089, 1753
HpyCH4III ACNGT 5 cut(s) 356, 415, 638, 1318, 1407
HpyCH4IV ACGT 3 cut(s) 405, 562, 991
HpyF3I CTNAG 7 cut(s) 51, 111, 870, 1190, 1629, 1638, 1750
HpySE526I ACGT 3 cut(s) 405, 562, 991
Hsp92I GRCGYC 1 cut(s) 1527
HspAI GCGC 2 cut(s) 130, 132
Kpn2I TCCGGA 1 cut(s) 757
KpnI GGTACC 1 cut(s) 486
Ksp22I TGATCA 2 cut(s) 846, 973
Kzo9I GATC 6 cut(s) 846, 917, 973, 1095, 1368, 1579
LmnI GCTCC 5 cut(s) 509, 1006, 1018, 1625, 1780
LweI GCATC 7 cut(s) 303, 575, 988, 1294, 1343, 1550, 1703
MaeI CTAG 3 cut(s) 287, 1500, 1691
MaeII ACGT 3 cut(s) 405, 562, 991
MaeIII GTNAC 5 cut(s) 46, 304, 398, 625, 1432
MalI GATC 6 cut(s) 848, 919, 975, 1097, 1370, 1581
MboI GATC 6 cut(s) 846, 917, 973, 1095, 1368, 1579
MboII GAAGA 3 cut(s) 1289, 1356, 1544
MfeI CAATTG 1 cut(s) 1158
MflI RGATCY 1 cut(s) 1368
MlyI GAGTC 1 cut(s) 1640
MmeI TCCRAC 3 cut(s) 446, 559, 1028
MroI TCCGGA 1 cut(s) 757
MseI TTAA 1 cut(s) 1565
MspA1I CMGCKG 4 cut(s) 518, 880, 1169, 1310
MspI CCGG 6 cut(s) 144, 272, 480, 686, 758, 915
MspR9I CCNGG 6 cut(s) 11, 84, 618, 648, 687, 1710
MunI CAATTG 1 cut(s) 1158
MvaI CCWGG 5 cut(s) 11, 84, 618, 648, 1710
MvnI CGCG 1 cut(s) 132
NciI CCSGG 1 cut(s) 687
NdeII GATC 6 cut(s) 846, 917, 973, 1095, 1368, 1579
NlaIV GGNNCC 6 cut(s) 353, 484, 645, 1014, 1370, 1776
NmuCI GTSAC 3 cut(s) 46, 625, 1432
NspI RCATGY 3 cut(s) 209, 1285, 1648
PaeR7I CTCGAG 1 cut(s) 450
PagI TCATGA 2 cut(s) 924, 963
PasI CCCWGGG 1 cut(s) 83
PauI GCGCGC 1 cut(s) 130
PfeI GAWTC 2 cut(s) 278, 1060
PfoI TCCNGGA 1 cut(s) 9
PinAI ACCGGT 1 cut(s) 271
PleI GAGTC 1 cut(s) 1639
PpsI GAGTC 1 cut(s) 1639
PpuMI RGGWCCY 1 cut(s) 644
Psp5II RGGWCCY 1 cut(s) 644
Psp6I CCWGG 5 cut(s) 9, 82, 616, 646, 1708
PspFI CCCAGC 2 cut(s) 1505, 1658
PspGI CCWGG 5 cut(s) 9, 82, 616, 646, 1708
PspN4I GGNNCC 6 cut(s) 353, 484, 645, 1014, 1370, 1776
PspPI GGNCC 3 cut(s) 311, 352, 644
PspPPI RGGWCCY 1 cut(s) 644
PspXI VCTCGAGB 1 cut(s) 450
PstI CTGCAG 6 cut(s) 93, 379, 517, 1480, 1618, 1726
PstNI CAGNNNCTG 1 cut(s) 1622
PsuI RGATCY 1 cut(s) 1368
PteI GCGCGC 1 cut(s) 130
PvuII CAGCTG 3 cut(s) 518, 880, 1169
RsaI GTAC 4 cut(s) 95, 484, 867, 1736
RsaNI GTAC 4 cut(s) 94, 483, 866, 1735
SalI GTCGAC 1 cut(s) 1113
SaqAI TTAA 1 cut(s) 1565
Sau3AI GATC 6 cut(s) 846, 917, 973, 1095, 1368, 1579
Sau96I GGNCC 3 cut(s) 311, 352, 644
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 1 cut(s) 1640
ScrFI CCNGG 6 cut(s) 11, 84, 618, 648, 687, 1710
SfaNI GCATC 7 cut(s) 303, 575, 988, 1294, 1343, 1550, 1703
SfcI CTRYAG 6 cut(s) 89, 375, 513, 1476, 1614, 1722
Sfr274I CTCGAG 1 cut(s) 450
SinI GGWCC 3 cut(s) 311, 352, 644
SlaI CTCGAG 1 cut(s) 450
SmlI CTYRAG 3 cut(s) 450, 740, 1148
SmoI CTYRAG 3 cut(s) 450, 740, 1148
SsiI CCGC 8 cut(s) 201, 249, 290, 446, 831, 1053, 1239, 1310
SspMI CTAG 3 cut(s) 287, 1500, 1691
StyD4I CCNGG 6 cut(s) 9, 82, 616, 646, 685, 1708
TaaI ACNGT 5 cut(s) 356, 415, 638, 1318, 1407
TaiI ACGT 3 cut(s) 408, 565, 994
TaqI TCGA 6 cut(s) 451, 953, 1098, 1114, 1555, 1667
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 2 cut(s) 278, 1060
Tru1I TTAA 1 cut(s) 1565
Tru9I TTAA 1 cut(s) 1565
TscAI CASTG 2 cut(s) 618, 1323
TseFI GTSAC 3 cut(s) 46, 625, 1432
Tsp45I GTSAC 3 cut(s) 46, 625, 1432
TspDTI ATGAA 8 cut(s) 46, 210, 485, 665, 913, 980, 1146, 1212
TspRI CASTG 2 cut(s) 618, 1323
VpaK11BI GGWCC 3 cut(s) 311, 352, 644
XapI RAATTY 2 cut(s) 361, 672
XceI RCATGY 3 cut(s) 209, 1285, 1648
XhoI CTCGAG 1 cut(s) 450
XmiI GTMKAC 2 cut(s) 1114, 1376
XspI CTAG 3 cut(s) 287, 1500, 1691
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.