pycom14g20240

3,4-dihydroxy-2-butanone kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012302
Physical Location & Seq
Forward (+)
21302120 .. 21307045
4926 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g20240.7

Sequence Viewer

Length: 1779 bp
ATGGCTTTTCAAGGCAAGAAGCTCATCAACGACCCCAACGATGTGGTGACTGAGTTCATCGAGGGGCTTGTCGAAACGTATCCTGGGCTGCAGTACTTGGATGGTTTTCCTCAGGTGAAGGTTGTGTTGCGTGCTGATGTCTCTGGTGGGACGTATGACAAAGTTGCGATTATATCAGGAGGTGGAAGTGGGCATGAGCCTGCACAATCTGGATTTGTTGGAGAAGGGATGCTAACTGCAGCTATTTGCGGGGATGTTTTTACCTCACCACAAGTTGATTCAATTCTAGCAGGCATCCGAGCTGTGACTGGTCCTTCGGGTTGTCTTCTGATTGTTACGAATTATACTGGGGACCGTTTAAATTTTGGTTTAGCTGCAGAGCAAGCAAAATCTGAAGGTTATAAAGTAGAGACTGTAATTGTTGGAGATGATTGTGCATTACCCCCACCTCGAGGAATAGCTGGTCGAAGGGGTTTGGCCGGGACCATTCTTGTTCATAAGATTGCTGGAGCTGCCGCTGCTGCTGGCCTTTCACTTGCTGATGTTGCTGCAGAAGCAAGACGAGCATCTGAAATGGTTGGAACAATGGGCGTTGCACTATCTGTTTGCACATTGCCTGGTCAGGTTACACCAGACCGTTTGGGTCCAGGAAAGATGGAAGTTGGTCTTGGAATTCATGGAGAACCTGGTGCTGCTGTAGCTGATCTTCAACCGGTGGATGTTATAGTTTCTCACGTTCTTAAGCAGATATTGTCTCCAGAAACTAATTATGTTCCAATTACTCGGGGTAGTAGAGTGGTGCTTATGGTAAACGGGTTAGGTGCGACTCCGGGAATGGAACTGATGATTGCATCTGGTAAAGCAGTACCTAAGTTGCAGCTGGAACATGGATTGGCAGTGGATAGAGTGTATACTGGGTCGTTTATGACTTCTCTTGATATGGCAGGTTTTTCGATTTCTATCATGAAGGCTCGATCAAACTATTTTGCAACGTCTGGATGCTGTAACAAAGGCGCCATATTGGCCTGTTGGTGCAATCACCCACCTGCAAAGATTCCTGTTCCATTACCTCCATCTCGTTCAACAAAGTCTGATGAGCCATTAGGTCGGCCAGCACAACTTAATGAACAAGGCCAGATTCTTGAGGTGGCCATTGAAGCAGCTGCAAATGCAATTAAAAAACTTAAGGACTATTTGAATGATTGGGATGGCAAAGTAGGTGATGGTGACTGTGGGTCAACAATGTATAGAGGTGCAACAGCAGTTTTGGAAGACATGAAAAATTATCCTCTGAATGATGCAACCGAAACAGTAAACGAAATTGGATCATCTATCAGAAGAGATATGGGGGGTACAAGTGGGATCCTGTATGTTATATTTTGTAAGGCTGCCTACGCTCAATTGAAAGCAAGCACCCAATCAGTTGTCACCTCAAAGAATTGGGCTGAAGCGCTTGAAGCTTCCATTGCTGCAGTCAGTAAATATGGTGGGGCTAGTGCTGGTTACCGGACAATGTTAGATGCCCTTATTCCAGCATCTACGGTTCTTCAGGAGAGTTTAAAGGCTGGTGATGATCCTGTGACTGCTTTTCTACTCTCATCTGAAGCGGCGTTAGGTGGAGCTGAGTCGACTAAGAACATGCAGGCAGAGGCTGGTTGTTCAAGTTATATCTCTGGAGATATACTGGCTTCAGTTCCAGACCCAGGTGCGATGGCTGCAGCGTCGTGGTACAGAGCAGCAGCCTTAGCTGTCAAGTACAAAAACCAGACTCCTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00561 ko00680 ko01100 ko01120 ko01200 ko04622 map00051 map00561 map00680 map01100 map01120 map01200 map04622
Pfam Domains
Protein Families
Dak1 Dak2

Protein Analysis

593

Amino Acids

61.31

Weight (kDa)

5.3

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012302)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48430 AT1G48430 AT3G17770
fragaria_vesca FvH4_5g17820
malus_domestica MD06G1234200.v1.1 MD14G1240900.v1.1
prunus_persica Prupe.5G238700_v2.0.a1
pyrus_communis pycom06g20890 pycom14g20240
rosa_chinensis RchiOBHm_Chr7g0177761
rosa_laevigata RLG00000005506
rosa_multiflora Rmu_sc0006214.1_g000015
rosa_roxburghii Rroxscaffold_3G00275580
rosa_rugosa Rorug06G0409300
rosa_samantha Rh7AG008900 Rh7BG009000 Rh7CG009200 Rh7DG009000
rosa_wichuraiana Rw7G000730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AarI CACCTGC 1 cut(s) 1054
AbsI CCTCGAGG 1 cut(s) 450
Acc36I ACCTGC 2 cut(s) 935, 1054
AccB1I GGYRCC 1 cut(s) 1013
AccI GTMKAC 2 cut(s) 911, 1628
AciI CCGC 3 cut(s) 249, 516, 1607
AclWI GGATC 4 cut(s) 1333, 1357, 1370, 1568
AcoI YGGCCR 3 cut(s) 477, 1109, 1149
AcsI RAATTY 2 cut(s) 361, 672
AcuI CTGAAG 5 cut(s) 414, 1467, 1532, 1623, 1674
AcyI GRCGYC 1 cut(s) 1014
AfaI GTAC 5 cut(s) 95, 867, 1354, 1730, 1757
AfeI AGCGCT 1 cut(s) 1452
AfiI CCNNNNNNNGG 2 cut(s) 452, 1703
AflII CTTAAG 2 cut(s) 740, 1184
AgeI ACCGGT 1 cut(s) 712
AgsI TTSAA 9 cut(s) 11, 282, 710, 1083, 1157, 1198, 1405, 1457, 1662
AhdI GACNNNNNGTC 1 cut(s) 1234
AjnI CCWGG 5 cut(s) 82, 616, 646, 685, 1702
AjuI GAANNNNNNNTTGG 2 cut(s) 651, 683
Alw26I GTCTC 3 cut(s) 145, 404, 759
AlwI GGATC 4 cut(s) 1333, 1357, 1370, 1568
AlwNI CAGNNNCTG 1 cut(s) 1652
Ama87I CYCGRG 2 cut(s) 450, 783
Aor51HI AGCGCT 1 cut(s) 1452
AoxI GGCC 6 cut(s) 477, 526, 1023, 1109, 1132, 1149
ApoI RAATTY 2 cut(s) 361, 672
ArsI GACNNNNNNTTYG 2 cut(s) 298, 330
AsiGI ACCGGT 1 cut(s) 712
AspLEI GCGC 2 cut(s) 1016, 1453
AspS9I GGNCC 4 cut(s) 311, 352, 483, 644
AsuC2I CCSGG 2 cut(s) 481, 831
AsuHPI GGTGA 8 cut(s) 58, 127, 258, 1031, 1232, 1238, 1420, 1580
AvaI CYCGRG 2 cut(s) 450, 783
AvaII GGWCC 4 cut(s) 311, 352, 483, 644
AxyI CCTNAGG 1 cut(s) 111
BalI TGGCCA 1 cut(s) 1151
BamHI GGATCC 1 cut(s) 1362
BanI GGYRCC 1 cut(s) 1013
BarI GAAGNNNNNNTAC 2 cut(s) 690, 722
BbsI GAAGAC 2 cut(s) 317, 1278
BccI CCATC 6 cut(s) 95, 649, 1081, 1202, 1217, 1705
BcgI CGANNNNNNTGC 2 cut(s) 933, 967
BciT130I CCWGG 5 cut(s) 84, 618, 648, 687, 1704
BciVI GTATCC 1 cut(s) 90
BcnI CCSGG 2 cut(s) 481, 831
BcoDI GTCTC 3 cut(s) 145, 404, 759
BfaI CTAG 2 cut(s) 287, 1494
BfmI CTRYAG 7 cut(s) 89, 237, 375, 549, 696, 1470, 1716
BfoI RGCGCY 2 cut(s) 1017, 1454
BfrI CTTAAG 2 cut(s) 740, 1184
BfuAI ACCTGC 2 cut(s) 935, 1054
BfuI GTATCC 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 1022
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1704
Bme18I GGWCC 4 cut(s) 311, 352, 483, 644
BmeRI GACNNNNNGTC 1 cut(s) 1234
BmeT110I CYCGRG 2 cut(s) 450, 783
BmgT120I GGNCC 4 cut(s) 311, 352, 483, 644
BmiI GGNNCC 5 cut(s) 353, 484, 645, 1015, 1364
BmrFI CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1704
BmrI ACTGGG 2 cut(s) 357, 924
BmsI GCATC 8 cut(s) 219, 303, 575, 860, 989, 1288, 1510, 1544
BmuI ACTGGG 2 cut(s) 357, 924
BpiI GAAGAC 2 cut(s) 317, 1278
BpmI CTGGAG 3 cut(s) 528, 741, 1695
Bpu10I CCTNAGC 1 cut(s) 1744
BpuEI CTTGAG 1 cut(s) 1163
BpuMI CCSGG 2 cut(s) 481, 831
BsaBI GATNNNNATC 1 cut(s) 959
BsaHI GRCGYC 1 cut(s) 1014
BsaJI CCNNGG 2 cut(s) 83, 1702
BsaWI WCCGGW 2 cut(s) 712, 1506
Bsc4I CCNNNNNNNGG 2 cut(s) 452, 1703
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 4 cut(s) 313, 352, 919, 1689
Bse21I CCTNAGG 1 cut(s) 111
Bse3DI GCAATG 2 cut(s) 611, 1464
Bse8I GATNNNNATC 1 cut(s) 959
BseBI CCWGG 5 cut(s) 84, 618, 648, 687, 1704
BseDI CCNNGG 2 cut(s) 83, 1702
BseGI GGATG 7 cut(s) 106, 234, 259, 294, 724, 1004, 1213
BseJI GATNNNNATC 1 cut(s) 959
BseLI CCNNNNNNNGG 2 cut(s) 452, 1703
BseMI GCAATG 2 cut(s) 611, 1464
BseMII CTCAG 3 cut(s) 42, 125, 1614
BseNI ACTGG 4 cut(s) 313, 352, 919, 1689
BseRI GAGGAG 1 cut(s) 1761
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 6 cut(s) 479, 528, 1025, 1111, 1134, 1151
BshNI GGYRCC 1 cut(s) 1013
BshTI ACCGGT 1 cut(s) 712
BsiHKCI CYCGRG 2 cut(s) 450, 783
BsiSI CCGG 4 cut(s) 480, 713, 830, 1507
BslFI GGGAC 3 cut(s) 163, 365, 496
BslI CCNNNNNNNGG 2 cut(s) 452, 1703
BsmAI GTCTC 3 cut(s) 145, 404, 759
BsmFI GGGAC 3 cut(s) 163, 365, 496
BsnI GGCC 6 cut(s) 479, 528, 1025, 1111, 1134, 1151
BsoBI CYCGRG 2 cut(s) 450, 783
Bsp143I GATC 5 cut(s) 703, 974, 1325, 1362, 1573
BspACI CCGC 3 cut(s) 249, 516, 1607
BspANI GGCC 6 cut(s) 479, 528, 1025, 1111, 1134, 1151
BspCNI CTCAG 3 cut(s) 43, 124, 1615
BspHI TCATGA 1 cut(s) 963
BspLI GGNNCC 5 cut(s) 353, 484, 645, 1015, 1364
BspMAI CTGCAG 6 cut(s) 93, 241, 379, 553, 1474, 1720
BspMI ACCTGC 2 cut(s) 935, 1054
BspPI GGATC 4 cut(s) 1333, 1357, 1370, 1568
BspT107I GGYRCC 1 cut(s) 1013
BspTI CTTAAG 2 cut(s) 740, 1184
BsrDI GCAATG 2 cut(s) 611, 1464
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 4 cut(s) 313, 352, 919, 1689
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 2 cut(s) 83, 1702
BssMI GATC 5 cut(s) 703, 974, 1325, 1362, 1573
BssNAI GTATAC 1 cut(s) 912
BssNI GRCGYC 1 cut(s) 1014
Bst1107I GTATAC 1 cut(s) 912
Bst2UI CCWGG 5 cut(s) 84, 618, 648, 687, 1704
Bst4CI ACNGT 6 cut(s) 356, 415, 638, 1232, 1312, 1543
Bst6I CTCTTC 1 cut(s) 1333
BstACI GRCGYC 1 cut(s) 1014
BstAFI CTTAAG 2 cut(s) 740, 1184
BstC8I GCNNGC 8 cut(s) 132, 201, 292, 384, 526, 1113, 1411, 1644
BstDEI CTNAG 6 cut(s) 51, 111, 870, 1623, 1632, 1744
BstEII GGTNACC 1 cut(s) 1502
BstF5I GGATG 7 cut(s) 106, 234, 259, 294, 724, 1004, 1213
BstH2I RGCGCY 2 cut(s) 1017, 1454
BstHHI GCGC 2 cut(s) 1016, 1453
BstKTI GATC 5 cut(s) 706, 977, 1328, 1365, 1576
BstMAI GTCTC 3 cut(s) 145, 404, 759
BstMBI GATC 5 cut(s) 703, 974, 1325, 1362, 1573
BstNI CCWGG 5 cut(s) 84, 618, 648, 687, 1704
BstNSI RCATGY 1 cut(s) 1642
BstPI GGTNACC 1 cut(s) 1502
BstSCI CCNGG 7 cut(s) 82, 479, 616, 646, 685, 829, 1702
BstSFI CTRYAG 7 cut(s) 89, 237, 375, 549, 696, 1470, 1716
BstV2I GAAGAC 2 cut(s) 317, 1278
BstX2I RGATCY 1 cut(s) 1362
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 1362
BstZ17I GTATAC 1 cut(s) 912
Bsu36I CCTNAGG 1 cut(s) 111
BsuI GTATCC 1 cut(s) 90
BsuRI GGCC 6 cut(s) 479, 528, 1025, 1111, 1134, 1151
BtgZI GCGATG 1 cut(s) 1724
BtsCI GGATG 7 cut(s) 106, 234, 259, 294, 724, 1004, 1213
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 1 cut(s) 903
BveI ACCTGC 2 cut(s) 935, 1054
Cac8I GCNNGC 8 cut(s) 132, 201, 292, 384, 526, 1113, 1411, 1644
CaiI CAGNNNCTG 1 cut(s) 1652
CciI TCATGA 1 cut(s) 963
CfoI GCGC 2 cut(s) 1016, 1453
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 4 cut(s) 311, 352, 483, 644
CseI GACGC 1 cut(s) 1710
CsiI ACCWGGT 1 cut(s) 685
Csp6I GTAC 5 cut(s) 94, 866, 1353, 1729, 1756
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 6 cut(s) 194, 677, 887, 964, 1276, 1639
CviQI GTAC 5 cut(s) 94, 866, 1353, 1729, 1756
DdeI CTNAG 6 cut(s) 51, 111, 870, 1623, 1632, 1744
DinI GGCGCC 1 cut(s) 1015
DpnI GATC 5 cut(s) 705, 976, 1327, 1364, 1575
DpnII GATC 5 cut(s) 703, 974, 1325, 1362, 1573
DraI TTTAAA 2 cut(s) 360, 1560
DriI GACNNNNNGTC 1 cut(s) 1234
EaeI YGGCCR 3 cut(s) 477, 1109, 1149
Eam1104I CTCTTC 1 cut(s) 1333
Eam1105I GACNNNNNGTC 1 cut(s) 1234
EarI CTCTTC 1 cut(s) 1333
Eco47I GGWCC 4 cut(s) 311, 352, 483, 644
Eco47III AGCGCT 1 cut(s) 1452
Eco57I CTGAAG 5 cut(s) 414, 1467, 1532, 1623, 1674
Eco81I CCTNAGG 1 cut(s) 111
Eco88I CYCGRG 2 cut(s) 450, 783
Eco91I GGTNACC 1 cut(s) 1502
EcoO65I GGTNACC 1 cut(s) 1502
EcoRI GAATTC 1 cut(s) 672
EcoRII CCWGG 5 cut(s) 82, 616, 646, 685, 1702
EgeI GGCGCC 1 cut(s) 1015
EheI GGCGCC 1 cut(s) 1015
FaeI CATG 6 cut(s) 197, 680, 890, 967, 1279, 1642
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FaqI GGGAC 3 cut(s) 163, 365, 496
FatI CATG 6 cut(s) 193, 676, 886, 963, 1275, 1638
FauI CCCGC 1 cut(s) 242
FblI GTMKAC 2 cut(s) 911, 1628
FokI GGATG 7 cut(s) 113, 241, 266, 281, 731, 1011, 1220
FspBI CTAG 2 cut(s) 287, 1494
GlaI GCGC 2 cut(s) 1015, 1452
GsuI CTGGAG 3 cut(s) 528, 741, 1695
HaeII RGCGCY 2 cut(s) 1017, 1454
HaeIII GGCC 6 cut(s) 479, 528, 1025, 1111, 1134, 1151
HapII CCGG 4 cut(s) 480, 713, 830, 1507
HgaI GACGC 1 cut(s) 1710
HhaI GCGC 2 cut(s) 1016, 1453
Hin1I GRCGYC 1 cut(s) 1014
Hin1II CATG 6 cut(s) 197, 680, 890, 967, 1279, 1642
Hin6I GCGC 2 cut(s) 1014, 1451
HinP1I GCGC 2 cut(s) 1014, 1451
HincII GTYRAC 2 cut(s) 1239, 1629
HindII GTYRAC 2 cut(s) 1239, 1629
HindIII AAGCTT 1 cut(s) 1458
HinfI GANTC 6 cut(s) 278, 826, 1054, 1138, 1625, 1768
HpaII CCGG 4 cut(s) 480, 713, 830, 1507
HphI GGTGA 8 cut(s) 58, 127, 258, 1031, 1232, 1238, 1420, 1580
Hpy166II GTNNAC 5 cut(s) 811, 912, 1239, 1315, 1629
Hpy188I TCNGA 8 cut(s) 299, 330, 394, 571, 1093, 1293, 1337, 1603
Hpy8I GTNNAC 5 cut(s) 811, 912, 1239, 1315, 1629
Hpy99I CGWCG 1 cut(s) 1726
HpyAV CCTTC 6 cut(s) 112, 218, 324, 389, 462, 961
HpyCH4III ACNGT 6 cut(s) 356, 415, 638, 1232, 1312, 1543
HpyCH4IV ACGT 4 cut(s) 77, 152, 735, 992
HpyF3I CTNAG 6 cut(s) 51, 111, 870, 1623, 1632, 1744
HpySE526I ACGT 4 cut(s) 77, 152, 735, 992
Hsp92I GRCGYC 1 cut(s) 1014
Hsp92II CATG 6 cut(s) 197, 680, 890, 967, 1279, 1642
HspAI GCGC 2 cut(s) 1014, 1451
KasI GGCGCC 1 cut(s) 1013
Kzo9I GATC 5 cut(s) 703, 974, 1325, 1362, 1573
LmnI GCTCC 2 cut(s) 509, 1619
LweI GCATC 8 cut(s) 219, 303, 575, 860, 989, 1288, 1510, 1544
MabI ACCWGGT 1 cut(s) 685
MaeI CTAG 2 cut(s) 287, 1494
MaeII ACGT 4 cut(s) 77, 152, 735, 992
MaeIII GTNAC 9 cut(s) 46, 304, 334, 625, 1004, 1226, 1426, 1502, 1579
MalI GATC 5 cut(s) 705, 976, 1327, 1364, 1575
MboI GATC 5 cut(s) 703, 974, 1325, 1362, 1573
MboII GAAGA 5 cut(s) 317, 698, 1283, 1350, 1538
MfeI CAATTG 1 cut(s) 1400
MflI RGATCY 1 cut(s) 1362
MlsI TGGCCA 1 cut(s) 1151
MluNI TGGCCA 1 cut(s) 1151
Mly113I GGCGCC 1 cut(s) 1014
MlyI GAGTC 3 cut(s) 820, 1634, 1762
MmeI TCCRAC 3 cut(s) 199, 403, 559
Mox20I TGGCCA 1 cut(s) 1151
MscI TGGCCA 1 cut(s) 1151
MseI TTAA 6 cut(s) 359, 741, 1122, 1176, 1185, 1559
Msp20I TGGCCA 1 cut(s) 1151
MspA1I CMGCKG 3 cut(s) 518, 880, 1163
MspCI CTTAAG 2 cut(s) 740, 1184
MspI CCGG 4 cut(s) 480, 713, 830, 1507
MspR9I CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1704
MunI CAATTG 1 cut(s) 1400
MvaI CCWGG 5 cut(s) 84, 618, 648, 687, 1704
NarI GGCGCC 1 cut(s) 1014
NciI CCSGG 2 cut(s) 481, 831
NdeII GATC 5 cut(s) 703, 974, 1325, 1362, 1573
NlaIII CATG 6 cut(s) 197, 680, 890, 967, 1279, 1642
NlaIV GGNNCC 5 cut(s) 353, 484, 645, 1015, 1364
NmuCI GTSAC 5 cut(s) 46, 304, 1226, 1426, 1579
NspI RCATGY 1 cut(s) 1642
PaeR7I CTCGAG 1 cut(s) 450
PagI TCATGA 1 cut(s) 963
PaqCI CACCTGC 1 cut(s) 1054
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PfeI GAWTC 3 cut(s) 278, 1054, 1138
PfoI TCCNGGA 2 cut(s) 646, 829
PinAI ACCGGT 1 cut(s) 712
PleI GAGTC 3 cut(s) 820, 1633, 1762
PluTI GGCGCC 1 cut(s) 1017
PpsI GAGTC 3 cut(s) 820, 1633, 1762
PsiI TTATAA 1 cut(s) 402
Psp6I CCWGG 5 cut(s) 82, 616, 646, 685, 1702
PspEI GGTNACC 1 cut(s) 1502
PspGI CCWGG 5 cut(s) 82, 616, 646, 685, 1702
PspN4I GGNNCC 5 cut(s) 353, 484, 645, 1015, 1364
PspPI GGNCC 4 cut(s) 311, 352, 483, 644
PspXI VCTCGAGB 1 cut(s) 450
PstI CTGCAG 6 cut(s) 93, 241, 379, 553, 1474, 1720
PstNI CAGNNNCTG 1 cut(s) 1652
PsuI RGATCY 1 cut(s) 1362
PvuII CAGCTG 2 cut(s) 880, 1163
RsaI GTAC 5 cut(s) 95, 867, 1354, 1730, 1757
RsaNI GTAC 5 cut(s) 94, 866, 1353, 1729, 1756
SalI GTCGAC 1 cut(s) 1627
SaqAI TTAA 6 cut(s) 359, 741, 1122, 1176, 1185, 1559
Sau3AI GATC 5 cut(s) 703, 974, 1325, 1362, 1573
Sau96I GGNCC 4 cut(s) 311, 352, 483, 644
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 3 cut(s) 820, 1634, 1762
ScrFI CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1704
SexAI ACCWGGT 1 cut(s) 685
SfaNI GCATC 8 cut(s) 219, 303, 575, 860, 989, 1288, 1510, 1544
SfcI CTRYAG 7 cut(s) 89, 237, 375, 549, 696, 1470, 1716
SfoI GGCGCC 1 cut(s) 1015
Sfr274I CTCGAG 1 cut(s) 450
SinI GGWCC 4 cut(s) 311, 352, 483, 644
SlaI CTCGAG 1 cut(s) 450
SmlI CTYRAG 4 cut(s) 450, 740, 1142, 1184
SmoI CTYRAG 4 cut(s) 450, 740, 1142, 1184
SsiI CCGC 3 cut(s) 249, 516, 1607
SspDI GGCGCC 1 cut(s) 1013
SspMI CTAG 2 cut(s) 287, 1494
StyD4I CCNGG 7 cut(s) 82, 479, 616, 646, 685, 829, 1702
TaaI ACNGT 6 cut(s) 356, 415, 638, 1232, 1312, 1543
TaiI ACGT 4 cut(s) 80, 155, 738, 995
TaqI TCGA 7 cut(s) 60, 72, 451, 466, 953, 973, 1628
TatI WGTACW 2 cut(s) 93, 1755
TauI GCSGC 2 cut(s) 518, 1610
TfiI GAWTC 3 cut(s) 278, 1054, 1138
Tru1I TTAA 6 cut(s) 359, 741, 1122, 1176, 1185, 1559
Tru9I TTAA 6 cut(s) 359, 741, 1122, 1176, 1185, 1559
TscAI CASTG 1 cut(s) 903
TseFI GTSAC 5 cut(s) 46, 304, 1226, 1426, 1579
Tsp45I GTSAC 5 cut(s) 46, 304, 1226, 1426, 1579
TspDTI ATGAA 6 cut(s) 46, 485, 665, 980, 1140, 1292
TspRI CASTG 1 cut(s) 903
Vha464I CTTAAG 2 cut(s) 740, 1184
VpaK11BI GGWCC 4 cut(s) 311, 352, 483, 644
XapI RAATTY 2 cut(s) 361, 672
XceI RCATGY 1 cut(s) 1642
XhoI CTCGAG 1 cut(s) 450
XmiI GTMKAC 2 cut(s) 911, 1628
XspI CTAG 2 cut(s) 287, 1494
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.