MD14G1240900.v1.1

3,4-dihydroxy-2-butanone kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012302
Physical Location & Seq
Forward (+)
31972202 .. 31976610
4409 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1240900.v1.1.491

Sequence Viewer

Length: 1497 bp
ATGGGCATCCGAGCTGTGACTGGTCCTTCGGGTTGTCTTCTTATTGTCACGAATTATACTGGGGACCGTTTAAATTTTGGTTTAGCTGCAGAGCAAGCAAAATCAGAAGGTTATAAAGTAGAGACTGTAATTGTTGGAGATGATTGTGCATTACCCCCGACTCGAGGAATAGTTGGTCGAAGGGGTTTGGCCGGGACCATTCTTGTTCATAAGATTGCTGGAGCTGCTGCTGCTGCTGGCCTTTCACTTGCGGATGTTGCCGCAGAAGCAAGACGAGCATCTGAAATGGTTGGAACAATGGGCGTTGCACTATCTGTTTGCACATTGCCTGGTCAGGTTACACCAGACCGTTTGGGTCCAGGAAAGATGGAACTTGGTCTTGGAATTCATGGAGAACCTGGTGCTGCTGTAGCTGATCTTCAACCGGTGGATGTTATAGTTTCTCACGTTCTTAAGCAGATATTGTCTCCAGAAACTAATTATGTTCCAATTACTCGGGGTAGTAGAGTGGTGCTTATGGTAAACGGGTTAGGTGCGACTCCAGGAATGGAACTGATGATTGCATCTGGTAAAGCAGTGCCTAAGTTGCAGCTGGAACATGGATTGGCAGTGGATAGAGTGTATACTGGGTCGTTTATGACTTCTCTTGACATGGCAGGTTTTTCTATTTCTATCATGAAGGCTGATCAAACTATTTTGCAACGTCTGGATGCTGCAACAAAGGCGCCATATTGGCCTGTTGGTGTTGATGGCAATCACCCACCTGCAAAGATTCCTGTTCCATTACCTCCATCTCGTTCAACAAAGTCTGATGAGCCATTAGGTCGGCCAGCACAACTTAACGAACAAGGCCAGATTCTTGAGGTGGCCATTGAAGCAGCTGCAAATGCAATTAAAAAACTTAAGGACAATTTGAATGATTGGGATGGCAAAGTAGGTGATGGTGACTGTGGGTCAACAATGTATAGAGGTGCAACAGCAGTTTTGGAAGACATGAAAAATTATCCTCTGAATGATGCAACCGAAACAGTCAACGAAATTGGATCATCAATCAGAAGAGTTATGGGGGGCACAAGTGGGATCCTGTATGATATATTTTGTAAGGCAGCCTACGCTCAATTGAAAGCAAGCACCCAATCAGTTGTCACCTCAAAGAATTGGGCTGAAGCGCTTGAAGCTTCCATTGCTGCAGTGAGTAAATATGGTGGGGCTAGTGCTGGTTACCGGACAATGTTAGATGCCCTTATTCCAGCATCTACGGTTCTTCAGGAGAGGTTAAAGGCTGGTGATGATCCTGTGACTGCTTTTCTACTCTCATCTGAAGCGGCATTAGATGGAGCTGAGTCGACTAAGAACATGCAGGCACAGGCTGGTCGTTCAAGTTATATCTCTGGAGATATACTGGCTTCAGTTCCAGACCCAGGTGCGATGGCTGTAGCGTCGTGGTACGGAGCAGCAGCCTTTGCTGTCAAGTACAAAAACCAGACTCCTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00561 ko00680 ko01100 ko01120 ko01200 ko04622 map00051 map00561 map00680 map01100 map01120 map01200 map04622
Pfam Domains
Protein Families
Dak1 Dak2

Protein Analysis

499

Amino Acids

51.74

Weight (kDa)

5.7

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dak1 PF02733 3 - 243 2.7e-82 Dak1 domain
Dak2 PF02734 311 - 484 3.5e-41 DAK2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012302)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48430 AT1G48430 AT3G17770
fragaria_vesca FvH4_5g17820
malus_domestica MD06G1234200.v1.1 MD14G1240900.v1.1
prunus_persica Prupe.5G238700_v2.0.a1
pyrus_communis pycom06g20890 pycom14g20240
rosa_chinensis RchiOBHm_Chr7g0177761
rosa_laevigata RLG00000005506
rosa_multiflora Rmu_sc0006214.1_g000015
rosa_roxburghii Rroxscaffold_3G00275580
rosa_rugosa Rorug06G0409300
rosa_samantha Rh7AG008900 Rh7BG009000 Rh7CG009200 Rh7DG009000
rosa_wichuraiana Rw7G000730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 114
AarI CACCTGC 1 cut(s) 772
Acc36I ACCTGC 2 cut(s) 647, 772
AccB1I GGYRCC 1 cut(s) 724
AccI GTMKAC 2 cut(s) 623, 1346
AciI CCGC 3 cut(s) 251, 261, 1325
AclWI GGATC 4 cut(s) 1051, 1075, 1088, 1286
AcoI YGGCCR 3 cut(s) 189, 827, 867
AcsI RAATTY 2 cut(s) 73, 384
AcuI CTGAAG 4 cut(s) 1185, 1250, 1341, 1392
AcyI GRCGYC 1 cut(s) 725
AfaI GTAC 2 cut(s) 1448, 1475
AfeI AGCGCT 1 cut(s) 1170
AfiI CCNNNNNNNGG 2 cut(s) 164, 1421
AflII CTTAAG 2 cut(s) 452, 902
AgeI ACCGGT 1 cut(s) 424
AgsI TTSAA 7 cut(s) 422, 801, 875, 916, 1123, 1175, 1380
AhdI GACNNNNNGTC 1 cut(s) 952
AjnI CCWGG 5 cut(s) 328, 358, 397, 541, 1420
AjuI GAANNNNNNNTTGG 2 cut(s) 363, 395
AluBI AGCT 8 cut(s) 14, 86, 224, 413, 592, 881, 1178, 1340
AluI AGCT 8 cut(s) 14, 86, 224, 413, 592, 881, 1178, 1340
Alw26I GTCTC 2 cut(s) 116, 471
AlwI GGATC 4 cut(s) 1051, 1075, 1088, 1286
Ama87I CYCGRG 2 cut(s) 162, 495
Aor51HI AGCGCT 1 cut(s) 1170
AoxI GGCC 6 cut(s) 189, 238, 734, 827, 850, 867
ApoI RAATTY 2 cut(s) 73, 384
ArsI GACNNNNNNTTYG 2 cut(s) 10, 42
AsiGI ACCGGT 1 cut(s) 424
AspLEI GCGC 2 cut(s) 727, 1171
AspS9I GGNCC 4 cut(s) 23, 64, 195, 356
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 5 cut(s) 749, 950, 956, 1138, 1298
AvaI CYCGRG 2 cut(s) 162, 495
AvaII GGWCC 4 cut(s) 23, 64, 195, 356
BaeGI GKGCMC 1 cut(s) 1073
BalI TGGCCA 1 cut(s) 869
BamHI GGATCC 1 cut(s) 1080
BanI GGYRCC 1 cut(s) 724
BarI GAAGNNNNNNTAC 2 cut(s) 402, 434
BbsI GAAGAC 2 cut(s) 29, 996
BccI CCATC 7 cut(s) 361, 743, 799, 920, 935, 1328, 1423
BciT130I CCWGG 5 cut(s) 330, 360, 399, 543, 1422
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 2 cut(s) 116, 471
BfaI CTAG 1 cut(s) 1212
BfmI CTRYAG 4 cut(s) 87, 408, 1188, 1434
BfoI RGCGCY 2 cut(s) 728, 1172
BfrI CTTAAG 2 cut(s) 452, 902
BfuAI ACCTGC 2 cut(s) 647, 772
BglI GCCNNNNNGGC 1 cut(s) 733
Bme1390I CCNGG 6 cut(s) 193, 330, 360, 399, 543, 1422
Bme18I GGWCC 4 cut(s) 23, 64, 195, 356
BmeRI GACNNNNNGTC 1 cut(s) 952
BmeT110I CYCGRG 2 cut(s) 162, 495
BmgT120I GGNCC 4 cut(s) 23, 64, 195, 356
BmiI GGNNCC 5 cut(s) 65, 196, 357, 726, 1082
BmrFI CCNGG 6 cut(s) 193, 330, 360, 399, 543, 1422
BmrI ACTGGG 2 cut(s) 69, 636
BmsI GCATC 7 cut(s) 15, 287, 572, 700, 1006, 1228, 1262
BmuI ACTGGG 2 cut(s) 69, 636
BpiI GAAGAC 2 cut(s) 29, 996
BpmI CTGGAG 4 cut(s) 240, 453, 525, 1413
BpuEI CTTGAG 1 cut(s) 881
BpuMI CCSGG 1 cut(s) 193
BsaBI GATNNNNATC 1 cut(s) 753
BsaHI GRCGYC 1 cut(s) 725
BsaJI CCNNGG 1 cut(s) 1420
BsaWI WCCGGW 2 cut(s) 424, 1224
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 1421
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 4 cut(s) 25, 64, 631, 1407
Bse3DI GCAATG 2 cut(s) 323, 1182
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 5 cut(s) 330, 360, 399, 543, 1422
BseDI CCNNGG 1 cut(s) 1420
BseGI GGATG 5 cut(s) 6, 259, 436, 715, 931
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 2 cut(s) 164, 1421
BseMI GCAATG 2 cut(s) 323, 1182
BseMII CTCAG 1 cut(s) 1332
BseNI ACTGG 4 cut(s) 25, 64, 631, 1407
BseRI GAGGAG 1 cut(s) 1479
BseSI GKGCMC 1 cut(s) 1073
BshFI GGCC 6 cut(s) 191, 240, 736, 829, 852, 869
BshNI GGYRCC 1 cut(s) 724
BshTI ACCGGT 1 cut(s) 424
BsiHKCI CYCGRG 2 cut(s) 162, 495
BsiSI CCGG 3 cut(s) 192, 425, 1225
BslFI GGGAC 2 cut(s) 77, 208
BslI CCNNNNNNNGG 2 cut(s) 164, 1421
BsmAI GTCTC 2 cut(s) 116, 471
BsmFI GGGAC 2 cut(s) 77, 208
BsnI GGCC 6 cut(s) 191, 240, 736, 829, 852, 869
BsoBI CYCGRG 2 cut(s) 162, 495
Bsp1286I GDGCHC 1 cut(s) 1073
Bsp143I GATC 5 cut(s) 415, 685, 1043, 1080, 1291
BspACI CCGC 3 cut(s) 251, 261, 1325
BspANI GGCC 6 cut(s) 191, 240, 736, 829, 852, 869
BspCNI CTCAG 1 cut(s) 1333
BspHI TCATGA 1 cut(s) 675
BspLI GGNNCC 5 cut(s) 65, 196, 357, 726, 1082
BspMAI CTGCAG 2 cut(s) 91, 1192
BspMI ACCTGC 2 cut(s) 647, 772
BspPI GGATC 4 cut(s) 1051, 1075, 1088, 1286
BspT107I GGYRCC 1 cut(s) 724
BspTI CTTAAG 2 cut(s) 452, 902
BsrDI GCAATG 2 cut(s) 323, 1182
BsrFI RCCGGY 1 cut(s) 424
BsrI ACTGG 4 cut(s) 25, 64, 631, 1407
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 1420
BssMI GATC 5 cut(s) 415, 685, 1043, 1080, 1291
BssNAI GTATAC 1 cut(s) 624
BssNI GRCGYC 1 cut(s) 725
Bst1107I GTATAC 1 cut(s) 624
Bst2UI CCWGG 5 cut(s) 330, 360, 399, 543, 1422
Bst4CI ACNGT 6 cut(s) 68, 127, 350, 950, 1030, 1261
Bst6I CTCTTC 1 cut(s) 1051
BstACI GRCGYC 1 cut(s) 725
BstAFI CTTAAG 2 cut(s) 452, 902
BstAPI GCANNNNNTGC 1 cut(s) 1463
BstC8I GCNNGC 5 cut(s) 96, 238, 831, 1129, 1362
BstDEI CTNAG 3 cut(s) 582, 1341, 1350
BstEII GGTNACC 1 cut(s) 1220
BstF5I GGATG 5 cut(s) 6, 259, 436, 715, 931
BstH2I RGCGCY 2 cut(s) 728, 1172
BstHHI GCGC 2 cut(s) 727, 1171
BstKTI GATC 5 cut(s) 418, 688, 1046, 1083, 1294
BstMAI GTCTC 2 cut(s) 116, 471
BstMBI GATC 5 cut(s) 415, 685, 1043, 1080, 1291
BstNI CCWGG 5 cut(s) 330, 360, 399, 543, 1422
BstNSI RCATGY 1 cut(s) 1360
BstPI GGTNACC 1 cut(s) 1220
BstSCI CCNGG 6 cut(s) 191, 328, 358, 397, 541, 1420
BstSFI CTRYAG 4 cut(s) 87, 408, 1188, 1434
BstSLI GKGCMC 1 cut(s) 1073
BstV2I GAAGAC 2 cut(s) 29, 996
BstX2I RGATCY 1 cut(s) 1080
BstYI RGATCY 1 cut(s) 1080
BstZ17I GTATAC 1 cut(s) 624
BsuRI GGCC 6 cut(s) 191, 240, 736, 829, 852, 869
BtgZI GCGATG 1 cut(s) 1442
BtsCI GGATG 5 cut(s) 6, 259, 436, 715, 931
BtsI GCAGTG 3 cut(s) 582, 615, 1197
BtsIMutI CAGTG 3 cut(s) 582, 615, 1197
BveI ACCTGC 2 cut(s) 647, 772
Cac8I GCNNGC 5 cut(s) 96, 238, 831, 1129, 1362
CciI TCATGA 1 cut(s) 675
CfoI GCGC 2 cut(s) 727, 1171
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 4 cut(s) 23, 64, 195, 356
CseI GACGC 1 cut(s) 1428
CsiI ACCWGGT 1 cut(s) 397
Csp6I GTAC 2 cut(s) 1447, 1474
CspAI ACCGGT 1 cut(s) 424
CviAII CATG 6 cut(s) 389, 599, 652, 676, 994, 1357
CviQI GTAC 2 cut(s) 1447, 1474
DdeI CTNAG 3 cut(s) 582, 1341, 1350
DinI GGCGCC 1 cut(s) 726
DpnI GATC 5 cut(s) 417, 687, 1045, 1082, 1293
DpnII GATC 5 cut(s) 415, 685, 1043, 1080, 1291
DraI TTTAAA 1 cut(s) 72
DriI GACNNNNNGTC 1 cut(s) 952
EaeI YGGCCR 3 cut(s) 189, 827, 867
Eam1104I CTCTTC 1 cut(s) 1051
Eam1105I GACNNNNNGTC 1 cut(s) 952
EarI CTCTTC 1 cut(s) 1051
Eco47I GGWCC 4 cut(s) 23, 64, 195, 356
Eco47III AGCGCT 1 cut(s) 1170
Eco57I CTGAAG 4 cut(s) 1185, 1250, 1341, 1392
Eco88I CYCGRG 2 cut(s) 162, 495
Eco91I GGTNACC 1 cut(s) 1220
EcoO65I GGTNACC 1 cut(s) 1220
EcoRI GAATTC 1 cut(s) 384
EcoRII CCWGG 5 cut(s) 328, 358, 397, 541, 1420
EgeI GGCGCC 1 cut(s) 726
EheI GGCGCC 1 cut(s) 726
FaeI CATG 6 cut(s) 392, 602, 655, 679, 997, 1360
FaqI GGGAC 2 cut(s) 77, 208
FatI CATG 6 cut(s) 388, 598, 651, 675, 993, 1356
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 2 cut(s) 623, 1346
FokI GGATG 4 cut(s) 266, 443, 722, 938
FspBI CTAG 1 cut(s) 1212
GlaI GCGC 2 cut(s) 726, 1170
GsuI CTGGAG 4 cut(s) 240, 453, 525, 1413
HaeII RGCGCY 2 cut(s) 728, 1172
HaeIII GGCC 6 cut(s) 191, 240, 736, 829, 852, 869
HapII CCGG 3 cut(s) 192, 425, 1225
HgaI GACGC 1 cut(s) 1428
HhaI GCGC 2 cut(s) 727, 1171
Hin1I GRCGYC 1 cut(s) 725
Hin1II CATG 6 cut(s) 392, 602, 655, 679, 997, 1360
Hin6I GCGC 2 cut(s) 725, 1169
HinP1I GCGC 2 cut(s) 725, 1169
HincII GTYRAC 3 cut(s) 957, 1033, 1347
HindII GTYRAC 3 cut(s) 957, 1033, 1347
HindIII AAGCTT 1 cut(s) 1176
HinfI GANTC 6 cut(s) 160, 538, 772, 856, 1343, 1486
HpaII CCGG 3 cut(s) 192, 425, 1225
HphI GGTGA 5 cut(s) 749, 950, 956, 1138, 1298
Hpy166II GTNNAC 5 cut(s) 523, 624, 957, 1033, 1347
Hpy188I TCNGA 7 cut(s) 11, 106, 283, 811, 1011, 1055, 1321
Hpy188III TCNNGA 9 cut(s) 49, 470, 647, 676, 707, 860, 1268, 1392, 1415
Hpy8I GTNNAC 5 cut(s) 523, 624, 957, 1033, 1347
Hpy99I CGWCG 1 cut(s) 1444
HpyAV CCTTC 4 cut(s) 36, 101, 174, 673
HpyCH4III ACNGT 6 cut(s) 68, 127, 350, 950, 1030, 1261
HpyCH4IV ACGT 2 cut(s) 447, 703
HpyF3I CTNAG 3 cut(s) 582, 1341, 1350
HpySE526I ACGT 2 cut(s) 447, 703
Hsp92I GRCGYC 1 cut(s) 725
Hsp92II CATG 6 cut(s) 392, 602, 655, 679, 997, 1360
HspAI GCGC 2 cut(s) 725, 1169
KasI GGCGCC 1 cut(s) 724
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 5 cut(s) 415, 685, 1043, 1080, 1291
LmnI GCTCC 3 cut(s) 221, 1337, 1451
LweI GCATC 7 cut(s) 15, 287, 572, 700, 1006, 1228, 1262
MabI ACCWGGT 1 cut(s) 397
MaeI CTAG 1 cut(s) 1212
MaeII ACGT 2 cut(s) 447, 703
MaeIII GTNAC 7 cut(s) 16, 46, 337, 944, 1144, 1220, 1297
MalI GATC 5 cut(s) 417, 687, 1045, 1082, 1293
MboI GATC 5 cut(s) 415, 685, 1043, 1080, 1291
MboII GAAGA 5 cut(s) 29, 410, 1001, 1068, 1256
MfeI CAATTG 1 cut(s) 1118
MflI RGATCY 1 cut(s) 1080
MhlI GDGCHC 1 cut(s) 1073
MlsI TGGCCA 1 cut(s) 869
MluNI TGGCCA 1 cut(s) 869
Mly113I GGCGCC 1 cut(s) 725
MlyI GAGTC 4 cut(s) 154, 532, 1352, 1480
MmeI TCCRAC 2 cut(s) 115, 271
MnlI CCTC 7 cut(s) 158, 798, 856, 962, 1017, 1159, 1266
Mox20I TGGCCA 1 cut(s) 869
MscI TGGCCA 1 cut(s) 869
MseI TTAA 6 cut(s) 71, 453, 840, 894, 903, 1277
Msp20I TGGCCA 1 cut(s) 869
MspA1I CMGCKG 2 cut(s) 592, 881
MspCI CTTAAG 2 cut(s) 452, 902
MspI CCGG 3 cut(s) 192, 425, 1225
MspR9I CCNGG 6 cut(s) 193, 330, 360, 399, 543, 1422
MunI CAATTG 1 cut(s) 1118
MvaI CCWGG 5 cut(s) 330, 360, 399, 543, 1422
NarI GGCGCC 1 cut(s) 725
NciI CCSGG 1 cut(s) 193
NdeII GATC 5 cut(s) 415, 685, 1043, 1080, 1291
NlaIII CATG 6 cut(s) 392, 602, 655, 679, 997, 1360
NlaIV GGNNCC 5 cut(s) 65, 196, 357, 726, 1082
NmuCI GTSAC 5 cut(s) 16, 46, 944, 1144, 1297
NspI RCATGY 1 cut(s) 1360
PaeR7I CTCGAG 1 cut(s) 162
PagI TCATGA 1 cut(s) 675
PaqCI CACCTGC 1 cut(s) 772
PfeI GAWTC 2 cut(s) 772, 856
PfoI TCCNGGA 2 cut(s) 358, 541
PinAI ACCGGT 1 cut(s) 424
PleI GAGTC 4 cut(s) 154, 532, 1351, 1480
PluTI GGCGCC 1 cut(s) 728
PpsI GAGTC 4 cut(s) 154, 532, 1351, 1480
PsiI TTATAA 1 cut(s) 114
Psp6I CCWGG 5 cut(s) 328, 358, 397, 541, 1420
PspEI GGTNACC 1 cut(s) 1220
PspGI CCWGG 5 cut(s) 328, 358, 397, 541, 1420
PspN4I GGNNCC 5 cut(s) 65, 196, 357, 726, 1082
PspPI GGNCC 4 cut(s) 23, 64, 195, 356
PspXI VCTCGAGB 1 cut(s) 162
PstI CTGCAG 2 cut(s) 91, 1192
PsuI RGATCY 1 cut(s) 1080
PvuII CAGCTG 2 cut(s) 592, 881
RsaI GTAC 2 cut(s) 1448, 1475
RsaNI GTAC 2 cut(s) 1447, 1474
SalI GTCGAC 1 cut(s) 1345
SaqAI TTAA 6 cut(s) 71, 453, 840, 894, 903, 1277
Sau3AI GATC 5 cut(s) 415, 685, 1043, 1080, 1291
Sau96I GGNCC 4 cut(s) 23, 64, 195, 356
SchI GAGTC 4 cut(s) 154, 532, 1352, 1480
ScrFI CCNGG 6 cut(s) 193, 330, 360, 399, 543, 1422
SduI GDGCHC 1 cut(s) 1073
SexAI ACCWGGT 1 cut(s) 397
SfaNI GCATC 7 cut(s) 15, 287, 572, 700, 1006, 1228, 1262
SfcI CTRYAG 4 cut(s) 87, 408, 1188, 1434
SfoI GGCGCC 1 cut(s) 726
Sfr274I CTCGAG 1 cut(s) 162
SinI GGWCC 4 cut(s) 23, 64, 195, 356
SlaI CTCGAG 1 cut(s) 162
SmlI CTYRAG 4 cut(s) 162, 452, 860, 902
SmoI CTYRAG 4 cut(s) 162, 452, 860, 902
SsiI CCGC 3 cut(s) 251, 261, 1325
SspDI GGCGCC 1 cut(s) 724
SspMI CTAG 1 cut(s) 1212
StyD4I CCNGG 6 cut(s) 191, 328, 358, 397, 541, 1420
TaaI ACNGT 6 cut(s) 68, 127, 350, 950, 1030, 1261
TaiI ACGT 2 cut(s) 450, 706
TaqI TCGA 3 cut(s) 163, 178, 1346
TatI WGTACW 1 cut(s) 1473
TauI GCSGC 2 cut(s) 263, 1328
TfiI GAWTC 2 cut(s) 772, 856
Tru1I TTAA 6 cut(s) 71, 453, 840, 894, 903, 1277
Tru9I TTAA 6 cut(s) 71, 453, 840, 894, 903, 1277
TscAI CASTG 3 cut(s) 582, 615, 1197
TseFI GTSAC 5 cut(s) 16, 46, 944, 1144, 1297
Tsp45I GTSAC 5 cut(s) 16, 46, 944, 1144, 1297
TspDTI ATGAA 4 cut(s) 197, 377, 692, 1010
TspGWI ACGGA 1 cut(s) 1464
TspRI CASTG 3 cut(s) 582, 615, 1197
Vha464I CTTAAG 2 cut(s) 452, 902
VpaK11BI GGWCC 4 cut(s) 23, 64, 195, 356
XapI RAATTY 2 cut(s) 73, 384
XceI RCATGY 1 cut(s) 1360
XhoI CTCGAG 1 cut(s) 162
XmiI GTMKAC 2 cut(s) 623, 1346
XspI CTAG 1 cut(s) 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.