Rorug06G0409300

3,4-dihydroxy-2-butanone kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012302
Physical Location & Seq
Forward (+)
55179027 .. 55180885
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0409300.1

Sequence Viewer

Length: 1407 bp
ATGGAGGATGAGAAACCGAGATTTGTGAGGTGGTCGATAAAGGAGCTGTTCAACCTGCAGAAGCTCCATCAGAATCCAGACTGGCCAGAGGACTTGATAAAAGAAGGGCCGTGGTTAGAACATTGCTACATCAACTTGTCGGATACGGATGAGGAGCAGGAAACACCAAGTTTCAACAACTGGGTTCCCCAAGCGAGCCAAGACCAGGAAGAGGAGACCATAAGGGTTACTGGTAACATGAAAGTTCTGCTGAAAGAAATGGATAGGGTGATTGAAGACAATGGTGAAAAACAGGAAATGGAGGAGCAAATGAAGAAACTCTTGGAAGCAAATGAAGGACTTGCAAACCATATTAGGGTGCTTTGGAAAAAGGTTACTGTTGCTGATGGGAAAATTGAGAGTATGGAGAACAAAATGACTGAGGTGATGCAGGAAAACAGATCGCACCAGAACCACATAAAAGCACTGAAGAAATTGTTGGCGAAAGCAACCAGCAGGAACCCTGAACAGAAAAGCAGTGAGCAGCAAAATAATCAACTACAGATGGTGCTGGTGTCGACCGAGAAAGATCACAGGAGGGAATCTGAGGGGAACTTTGCAGAATATGACACTCGAACACCAGTCAACGAAGAACCAAGGGCAGCAGATCCAATTAATGCATCTCCAATATCTTACAAGAGTCCAGAACGGGAAGTGTTTGAGGATACACATGTGGACACACTAAATGAGATGGAATCAGAAAAAATAGATCAACTTGTGTCAAAAATACTGGAAGCAGCTGAGACGGTTGAAATGCCTGAGGCAGGAACAGAAATGGCAGTACCATCGGTCCCCAAGGAGAAGAAAACGGTTGACATGCATTCAATCGAGAGGAATGTGAAAGAAAAAAAGAGAAAGGCAGCTGTGAAGGACAAGGACGACGACTTTGAGTATGACACACCGGAGATCCTGAAGACTTATGGAAAGAGAAGAAAGACTGCTGCACAGGAACAGCCAAAGGAGAAAAAGAAAGCACAACCGAAGCCGCCAACAAAAATTCAGAAAGGAAAACAAGTGCAGCACCCAATGCAAATAAAAGATGTGAATTGTGAGAAGTACACTTGGAAAACATTAAAGCCTGAACTAGCAAGACACTACCAGCAATTCTTTGAGATAGTGGATGACAATACATATGTCTACTGGAGAAGTGATAATGGAATACTAGGGGTAACCAGGGGAGACATGAAGATTATCGTAGAAGAGAAAGATCTGGATGTTAATGTGGTCAAGGCTTACACTGAAATGTTGCAGAAGGAACTAAAAGAAAGGAACACTCAGATTGGATTACTAAACATCGAAGCAGCGGTAAGCACCGAAACAAAAATAGCAGCTGGCGTTGCACCACATCCGAAAAACATATTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00561 ko00680 ko01100 ko01120 ko01200 ko04622 map00051 map00561 map00680 map01100 map01120 map01200 map04622
Pfam Domains
Protein Families
Dak1 Dak2

Protein Analysis

468

Amino Acids

54.34

Weight (kDa)

5.51

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012302)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48430 AT1G48430 AT3G17770
fragaria_vesca FvH4_5g17820
malus_domestica MD06G1234200.v1.1 MD14G1240900.v1.1
prunus_persica Prupe.5G238700_v2.0.a1
pyrus_communis pycom06g20890 pycom14g20240
rosa_chinensis RchiOBHm_Chr7g0177761
rosa_laevigata RLG00000005506
rosa_multiflora Rmu_sc0006214.1_g000015
rosa_roxburghii Rroxscaffold_3G00275580
rosa_rugosa Rorug06G0409300
rosa_samantha Rh7AG008900 Rh7BG009000 Rh7CG009200 Rh7DG009000
rosa_wichuraiana Rw7G000730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 63
AccI GTMKAC 2 cut(s) 557, 1176
AciI CCGC 2 cut(s) 1025, 1343
AclWI GGATC 2 cut(s) 641, 940
AcoI YGGCCR 1 cut(s) 83
AcsI RAATTY 1 cut(s) 1035
AcuI CTGAAG 2 cut(s) 488, 971
AfaI GTAC 2 cut(s) 822, 1097
AfiI CCNNNNNNNGG 4 cut(s) 205, 211, 355, 803
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 5 cut(s) 52, 175, 275, 791, 864
AjnI CCWGG 2 cut(s) 204, 1211
AjuI GAANNNNNNNTTGG 4 cut(s) 305, 337, 461, 493
AluBI AGCT 5 cut(s) 46, 64, 779, 902, 1370
AluI AGCT 5 cut(s) 46, 64, 779, 902, 1370
Alw26I GTCTC 3 cut(s) 209, 776, 1212
AlwI GGATC 2 cut(s) 641, 940
AoxI GGCC 2 cut(s) 83, 107
ApeKI GCWGC 8 cut(s) 523, 641, 776, 899, 980, 1057, 1340, 1367
ApoI RAATTY 1 cut(s) 1035
ArsI GACNNNNNNTTYG 2 cut(s) 908, 940
AseI ATTAAT 1 cut(s) 654
AspS9I GGNCC 2 cut(s) 107, 829
AsuHPI GGTGA 3 cut(s) 280, 296, 436
AvaII GGWCC 1 cut(s) 829
AxyI CCTNAGG 1 cut(s) 798
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BalI TGGCCA 1 cut(s) 85
BbsI GAAGAC 2 cut(s) 282, 959
BbvI GCAGC 8 cut(s) 535, 653, 788, 911, 967, 1069, 1352, 1379
BccI CCATC 5 cut(s) 75, 380, 538, 724, 832
BceAI ACGGC 1 cut(s) 94
BcgI CGANNNNNNTGC 2 cut(s) 807, 841
BciT130I CCWGG 2 cut(s) 206, 1213
BciVI GTATCC 2 cut(s) 136, 697
BcoDI GTCTC 3 cut(s) 209, 776, 1212
BfaI CTAG 2 cut(s) 1124, 1202
BfmI CTRYAG 2 cut(s) 56, 539
BfuAI ACCTGC 1 cut(s) 63
BfuI GTATCC 2 cut(s) 136, 697
BglII AGATCT 1 cut(s) 1246
BisI GCNGC 9 cut(s) 524, 642, 777, 900, 981, 1025, 1058, 1341, 1368
BlsI GCNGC 9 cut(s) 525, 643, 778, 901, 982, 1026, 1059, 1342, 1369
Bme1390I CCNGG 2 cut(s) 206, 1213
Bme18I GGWCC 1 cut(s) 829
BmgT120I GGNCC 2 cut(s) 107, 829
BmiI GGNNCC 3 cut(s) 186, 500, 831
BmrFI CCNGG 2 cut(s) 206, 1213
BmrI ACTGGG 1 cut(s) 190
BmsI GCATC 2 cut(s) 417, 668
BmuI ACTGGG 1 cut(s) 190
BpiI GAAGAC 2 cut(s) 282, 959
BpmI CTGGAG 1 cut(s) 1201
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 4 cut(s) 110, 635, 834, 1212
BsaWI WCCGGW 1 cut(s) 940
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 211, 355, 803
Bse1I ACTGG 6 cut(s) 86, 185, 235, 620, 774, 1184
Bse21I CCTNAGG 1 cut(s) 798
Bse3DI GCAATG 1 cut(s) 121
BseBI CCWGG 2 cut(s) 206, 1213
BseDI CCNNGG 4 cut(s) 110, 635, 834, 1212
BseGI GGATG 5 cut(s) 13, 154, 1165, 1258, 1384
BseLI CCNNNNNNNGG 4 cut(s) 205, 211, 355, 803
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 5 cut(s) 411, 576, 771, 789, 1328
BseNI ACTGG 6 cut(s) 86, 185, 235, 620, 774, 1184
BseRI GAGGAG 3 cut(s) 167, 227, 317
BseXI GCAGC 8 cut(s) 535, 653, 788, 911, 967, 1069, 1352, 1379
BsgI GTGCAG 2 cut(s) 966, 1076
Bsh1285I CGRYCG 1 cut(s) 561
BshFI GGCC 2 cut(s) 85, 109
BsiEI CGRYCG 1 cut(s) 561
BsiSI CCGG 1 cut(s) 941
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 4 cut(s) 205, 211, 355, 803
BsmAI GTCTC 3 cut(s) 209, 776, 1212
BsmBI CGTCTC 1 cut(s) 776
BsmFI GGGAC 1 cut(s) 815
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 2 cut(s) 85, 109
Bso31I GGTCTC 1 cut(s) 209
Bsp143I GATC 6 cut(s) 440, 568, 646, 748, 945, 1246
BspACI CCGC 2 cut(s) 1025, 1343
BspANI GGCC 2 cut(s) 85, 109
BspCNI CTCAG 5 cut(s) 412, 577, 772, 790, 1327
BspLI GGNNCC 3 cut(s) 186, 500, 831
BspMAI CTGCAG 1 cut(s) 60
BspMI ACCTGC 1 cut(s) 63
BspPI GGATC 2 cut(s) 641, 940
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 121
BsrI ACTGG 6 cut(s) 86, 185, 235, 620, 774, 1184
BssECI CCNNGG 4 cut(s) 110, 635, 834, 1212
BssMI GATC 6 cut(s) 440, 568, 646, 748, 945, 1246
BssT1I CCWWGG 2 cut(s) 635, 834
Bst2UI CCWGG 2 cut(s) 206, 1213
Bst4CI ACNGT 3 cut(s) 379, 787, 850
Bst6I CTCTTC 2 cut(s) 204, 1233
BstAPI GCANNNNNTGC 1 cut(s) 1066
BstC8I GCNNGC 2 cut(s) 196, 1372
BstDEI CTNAG 5 cut(s) 420, 585, 780, 798, 1314
BstDSI CCRYGG 1 cut(s) 110
BstEII GGTNACC 1 cut(s) 1207
BstENI CCTNNNNNAGG 1 cut(s) 801
BstF5I GGATG 5 cut(s) 13, 154, 1165, 1258, 1384
BstKTI GATC 6 cut(s) 443, 571, 649, 751, 948, 1249
BstMAI GTCTC 3 cut(s) 209, 776, 1212
BstMBI GATC 6 cut(s) 440, 568, 646, 748, 945, 1246
BstMCI CGRYCG 1 cut(s) 561
BstMWI GCNNNNNNNGC 2 cut(s) 1066, 1376
BstNI CCWGG 2 cut(s) 206, 1213
BstNSI RCATGY 2 cut(s) 713, 859
BstPI GGTNACC 1 cut(s) 1207
BstSCI CCNGG 2 cut(s) 204, 1211
BstSFI CTRYAG 2 cut(s) 56, 539
BstV1I GCAGC 8 cut(s) 535, 653, 788, 911, 967, 1069, 1352, 1379
BstV2I GAAGAC 2 cut(s) 282, 959
BstX2I RGATCY 3 cut(s) 646, 945, 1246
BstYI RGATCY 3 cut(s) 646, 945, 1246
Bsu36I CCTNAGG 1 cut(s) 798
BsuI GTATCC 2 cut(s) 136, 697
BsuRI GGCC 2 cut(s) 85, 109
BtgI CCRYGG 1 cut(s) 110
BtsCI GGATG 5 cut(s) 13, 154, 1165, 1258, 1384
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 3 cut(s) 464, 523, 1275
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 2 cut(s) 196, 1372
Cfr13I GGNCC 2 cut(s) 107, 829
Csp6I GTAC 2 cut(s) 821, 1096
CviAII CATG 4 cut(s) 238, 710, 856, 1222
CviQI GTAC 2 cut(s) 821, 1096
DdeI CTNAG 5 cut(s) 420, 585, 780, 798, 1314
DpnI GATC 6 cut(s) 442, 570, 648, 750, 947, 1248
DpnII GATC 6 cut(s) 440, 568, 646, 748, 945, 1246
EaeI YGGCCR 1 cut(s) 83
Eam1104I CTCTTC 2 cut(s) 204, 1233
EarI CTCTTC 2 cut(s) 204, 1233
Eco130I CCWWGG 2 cut(s) 635, 834
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 829
Eco57I CTGAAG 2 cut(s) 488, 971
Eco81I CCTNAGG 1 cut(s) 798
Eco91I GGTNACC 1 cut(s) 1207
EcoNI CCTNNNNNAGG 1 cut(s) 801
EcoO65I GGTNACC 1 cut(s) 1207
EcoRII CCWGG 2 cut(s) 204, 1211
EcoT14I CCWWGG 2 cut(s) 635, 834
EcoT22I ATGCAT 2 cut(s) 661, 861
ErhI CCWWGG 2 cut(s) 635, 834
Esp3I CGTCTC 1 cut(s) 776
FaeI CATG 4 cut(s) 241, 713, 859, 1225
FalI AAGNNNNNCTT 2 cut(s) 305, 337
FaqI GGGAC 1 cut(s) 815
FatI CATG 4 cut(s) 237, 709, 855, 1221
FauNDI CATATG 1 cut(s) 1171
FblI GTMKAC 2 cut(s) 557, 1176
Fnu4HI GCNGC 9 cut(s) 524, 642, 777, 900, 981, 1025, 1058, 1341, 1368
FokI GGATG 5 cut(s) 20, 161, 1172, 1265, 1371
Fsp4HI GCNGC 9 cut(s) 524, 642, 777, 900, 981, 1025, 1058, 1341, 1368
FspBI CTAG 2 cut(s) 1124, 1202
GluI GCNGC 9 cut(s) 524, 642, 777, 900, 981, 1025, 1058, 1341, 1368
GsuI CTGGAG 1 cut(s) 1201
HaeIII GGCC 2 cut(s) 85, 109
HapII CCGG 1 cut(s) 941
Hin1II CATG 4 cut(s) 241, 713, 859, 1225
HincII GTYRAC 3 cut(s) 558, 625, 853
HindII GTYRAC 3 cut(s) 558, 625, 853
HinfI GANTC 4 cut(s) 73, 581, 679, 734
HpaII CCGG 1 cut(s) 941
HphI GGTGA 3 cut(s) 280, 296, 436
Hpy166II GTNNAC 6 cut(s) 558, 625, 715, 853, 1098, 1177
Hpy188I TCNGA 7 cut(s) 72, 142, 586, 739, 1041, 1317, 1389
Hpy188III TCNNGA 5 cut(s) 77, 683, 868, 949, 1250
Hpy8I GTNNAC 6 cut(s) 558, 625, 715, 853, 1098, 1177
Hpy99I CGWCG 1 cut(s) 923
HpyAV CCTTC 4 cut(s) 98, 329, 901, 1285
HpyCH4III ACNGT 3 cut(s) 379, 787, 850
HpyF10VI GCNNNNNNNGC 2 cut(s) 1066, 1376
HpyF3I CTNAG 5 cut(s) 420, 585, 780, 798, 1314
Hsp92II CATG 4 cut(s) 241, 713, 859, 1225
Kzo9I GATC 6 cut(s) 440, 568, 646, 748, 945, 1246
LmnI GCTCC 4 cut(s) 43, 69, 154, 304
Lsp1109I GCAGC 8 cut(s) 535, 653, 788, 911, 967, 1069, 1352, 1379
LweI GCATC 2 cut(s) 417, 668
MaeI CTAG 2 cut(s) 1124, 1202
MaeIII GTNAC 4 cut(s) 226, 233, 373, 1207
MalI GATC 6 cut(s) 442, 570, 648, 750, 947, 1248
MboI GATC 6 cut(s) 440, 568, 646, 748, 945, 1246
MflI RGATCY 3 cut(s) 646, 945, 1246
MlsI TGGCCA 1 cut(s) 85
MluCI AATT 6 cut(s) 393, 473, 651, 1035, 1084, 1142
MluNI TGGCCA 1 cut(s) 85
MlyI GAGTC 1 cut(s) 688
MmeI TCCRAC 1 cut(s) 120
Mox20I TGGCCA 1 cut(s) 85
Mph1103I ATGCAT 2 cut(s) 661, 861
MscI TGGCCA 1 cut(s) 85
MseI TTAA 4 cut(s) 654, 1112, 1257, 1401
MslI CAYNNNNRTG 1 cut(s) 1280
Msp20I TGGCCA 1 cut(s) 85
MspA1I CMGCKG 4 cut(s) 779, 902, 1343, 1370
MspI CCGG 1 cut(s) 941
MspR9I CCNGG 2 cut(s) 206, 1213
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 2 cut(s) 206, 1213
MwoI GCNNNNNNNGC 2 cut(s) 1066, 1376
NdeI CATATG 1 cut(s) 1171
NdeII GATC 6 cut(s) 440, 568, 646, 748, 945, 1246
NlaIII CATG 4 cut(s) 241, 713, 859, 1225
NlaIV GGNNCC 3 cut(s) 186, 500, 831
NsiI ATGCAT 2 cut(s) 661, 861
NspI RCATGY 2 cut(s) 713, 859
PciI ACATGT 1 cut(s) 709
PctI GAATGC 1 cut(s) 859
PfeI GAWTC 3 cut(s) 73, 581, 734
PkrI GCNGC 9 cut(s) 525, 643, 778, 901, 982, 1026, 1059, 1342, 1369
PleI GAGTC 1 cut(s) 687
PpsI GAGTC 1 cut(s) 687
PscI ACATGT 1 cut(s) 709
PshBI ATTAAT 1 cut(s) 654
Psp6I CCWGG 2 cut(s) 204, 1211
PspEI GGTNACC 1 cut(s) 1207
PspGI CCWGG 2 cut(s) 204, 1211
PspN4I GGNNCC 3 cut(s) 186, 500, 831
PspPI GGNCC 2 cut(s) 107, 829
PstI CTGCAG 1 cut(s) 60
PsuI RGATCY 3 cut(s) 646, 945, 1246
PvuII CAGCTG 3 cut(s) 779, 902, 1370
RsaI GTAC 2 cut(s) 822, 1097
RsaNI GTAC 2 cut(s) 821, 1096
RseI CAYNNNNRTG 1 cut(s) 1280
SalI GTCGAC 1 cut(s) 556
SaqAI TTAA 4 cut(s) 654, 1112, 1257, 1401
SatI GCNGC 9 cut(s) 524, 642, 777, 900, 981, 1025, 1058, 1341, 1368
Sau3AI GATC 6 cut(s) 440, 568, 646, 748, 945, 1246
Sau96I GGNCC 2 cut(s) 107, 829
SchI GAGTC 1 cut(s) 688
ScrFI CCNGG 2 cut(s) 206, 1213
SetI ASST 9 cut(s) 32, 48, 57, 66, 375, 426, 781, 904, 1372
SfaNI GCATC 2 cut(s) 417, 668
SfcI CTRYAG 2 cut(s) 56, 539
SinI GGWCC 1 cut(s) 829
SmiMI CAYNNNNRTG 1 cut(s) 1280
Sse9I AATT 6 cut(s) 393, 473, 651, 1035, 1084, 1142
SsiI CCGC 2 cut(s) 1025, 1343
SspMI CTAG 2 cut(s) 1124, 1202
StyD4I CCNGG 2 cut(s) 204, 1211
StyI CCWWGG 2 cut(s) 635, 834
TaaI ACNGT 3 cut(s) 379, 787, 850
TaqI TCGA 5 cut(s) 35, 557, 613, 867, 1335
TaqII GACCGA 2 cut(s) 575, 817
TasI AATT 6 cut(s) 393, 473, 651, 1035, 1084, 1142
TatI WGTACW 1 cut(s) 1095
TauI GCSGC 1 cut(s) 1027
TfiI GAWTC 3 cut(s) 73, 581, 734
Tru1I TTAA 4 cut(s) 654, 1112, 1257, 1401
Tru9I TTAA 4 cut(s) 654, 1112, 1257, 1401
TscAI CASTG 3 cut(s) 471, 523, 1282
TseI GCWGC 8 cut(s) 523, 641, 776, 899, 980, 1057, 1340, 1367
TspDTI ATGAA 4 cut(s) 254, 326, 348, 1238
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 3 cut(s) 471, 523, 1282
VpaK11BI GGWCC 1 cut(s) 829
VspI ATTAAT 1 cut(s) 654
XagI CCTNNNNNAGG 1 cut(s) 801
XapI RAATTY 1 cut(s) 1035
XceI RCATGY 2 cut(s) 713, 859
XmiI GTMKAC 2 cut(s) 557, 1176
XspI CTAG 2 cut(s) 1124, 1202
Zsp2I ATGCAT 2 cut(s) 661, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.