MD06G1234200.v1.1

3,4-dihydroxy-2-butanone kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012302
Physical Location & Seq
Forward (+)
36529076 .. 36534598
5523 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1234200.v1.1.491

Sequence Viewer

Length: 1785 bp
ATGGCTTTTCACGGCAAGAAGCTCATCAACGACCCCAACGATGTGGTGACTGAGTTCATCGAAGGGCTTGTCGAGACGTATCCTGGGCTGCAGTACTTGGATGGTTTCCCTCAGGTGAAGGTTGTGTTGCGTGCTGATGTTTCCGGTAGGACGTATGACAAAGTTGCGATTATATCAGGAGGTGGAAGTGGGCATGAGCCTGCACACGCTGGATTTGTGGGGGATGGAATGCTAACTGCAGCTATTTGTGGGGATGTTTTTGCCTCACCACAAGTTGATTCAATTCTAGCAGGTATCCGAGCTGTTACTGGTCCTTCAGGTTGTCTTCTGATTGTCAAGAATTATACTGGGGACCGTTTAAATTTTGGTGTAGCTGCAGAGCAAGCAAAATCAGAAGGTTACAAAGTAGAGACTGTAATTGTTGGAGATGATTGTGCATTACCCCCGCCTCGAGGAATAGCTGGTCGAAGGGGTTTGGCCGGGACCATTCTTGTTCATAAGATTGCTGGAGCTGCTGCTGCTGCTGGCCTTTCACTAGCTGATGTTGCCGCAGAAGCAAGACGAGCATCTGAATTGGTTGGAACAATGGGCGTTGCACTATCTGTTTGCACATTGCCTGGTCAGGTCACACCAGACCGTTTGGGTCCAGGAAAGATGGAACTTGGTCTTGGAATTCATGGAGAACCTGGTGCTGCTGTAGCTGATCTTCAACCGGTGGATGTTATAGTTTCTCACGTTCTTAAGCAGATATTGTCTCCAGAAACTAATTATGTTCCAATTACTCGGGGTTGTAGAGTGGTGCTTATGGTCAATGGGTTAGGTGCAACTCCTGGAATGGAGCTGATGATTGCATCTGGTAAAGCAGTACCTAAGTTGCAGCTGGAACATGGATTGGCAGTGGATAGAGTGTATACTGGGTCATTTATGACTTCTCTTGATATGGCAGGTTTTTCGATTTCTGTCATGAAGGCTGATCAAACTATTTTGCAACGTCTGGATGCAGCAACAAAGGCGCCATATTGGCCTGTTGGTGTTGATGGCAATCACCCACCAGCAAAGATTCCTGTTCCCTTACCTCCATCTCGTTCAATGAATTCTGATGAGCCATTAGGTCGGCCGGAACAGCTTAATGAACAAGGCCAGATTCTTGAGGTAGCCATTGAAGCAGCTGCAAATGCAATTAAAAAACTTAAGGACAATTTGAACGAATGGGATGGCAAAGTAGGTGATGGTGACTGTGGGTCAACAATGTATAGAGGTGCAACAACAGTTTTGGAAGACATGAAAAATTATCCTCTGAATGATGCAACTGAAACAGTGAACGAAATTGGATCGTCTATCAGAAGAGTAATGGGGGGCACAAGTGGGATCCTGTATGTTATATTTTGTAAGGCAGCCTACGCTCAGTTGAAATCAAGCACCCAATCAGTTGTCACGTCAAAAAATTGGGCTGAAGCGCTTGAAGCTTCCATTGCTGCAGTCAGTAAATATGGTGGGGCTAGTGCTGGTTATCGGACAATGTTAGATGCCCTTATTCCAGCATCTACGGTTCTTCAGGAGAGGTTAAAAGCTGGTGATGATCCTGTGACTGCTTTTTTACTCTCATCTGAAGCGGCATTAGATGGAGCTGAGTCGACTAAGAACATGGAAGCACAGGCTGGTCGTTCAAGTTATATCTCCGGAGATATACTCGCTTCAGTTCCAGACCCAGGTGCGATGGCAGCAGCGTCGTGGTACAGAGCAGCAGCCTTGGCTGTCAAGCACAAAAACCAGACTCCTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00561 ko00680 ko01100 ko01120 ko01200 ko04622 map00051 map00561 map00680 map01100 map01120 map01200 map04622
Pfam Domains
Protein Families
Dak1 Dak2

Protein Analysis

595

Amino Acids

61.85

Weight (kDa)

5.44

Isoelectric Point (pI)

30.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dak1 PF02733 22 - 339 4e-113 Dak1 domain
Dak2 PF02734 407 - 582 6.3e-41 DAK2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012302)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48430 AT1G48430 AT3G17770
fragaria_vesca FvH4_5g17820
malus_domestica MD06G1234200.v1.1 MD14G1240900.v1.1
prunus_persica Prupe.5G238700_v2.0.a1
pyrus_communis pycom06g20890 pycom14g20240
rosa_chinensis RchiOBHm_Chr7g0177761
rosa_laevigata RLG00000005506
rosa_multiflora Rmu_sc0006214.1_g000015
rosa_roxburghii Rroxscaffold_3G00275580
rosa_rugosa Rorug06G0409300
rosa_samantha Rh7AG008900 Rh7BG009000 Rh7CG009200 Rh7DG009000
rosa_wichuraiana Rw7G000730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 450
Acc36I ACCTGC 2 cut(s) 281, 935
AccB1I GGYRCC 1 cut(s) 1012
AccI GTMKAC 2 cut(s) 911, 1634
AccIII TCCGGA 1 cut(s) 1679
AciI CCGC 3 cut(s) 446, 549, 1613
AclWI GGATC 4 cut(s) 1339, 1363, 1376, 1574
AcoI YGGCCR 2 cut(s) 477, 1115
AcsI RAATTY 3 cut(s) 361, 672, 1093
AcuI CTGAAG 5 cut(s) 300, 1473, 1538, 1629, 1680
AcyI GRCGYC 1 cut(s) 1013
AfaI GTAC 3 cut(s) 95, 867, 1736
AfeI AGCGCT 1 cut(s) 1458
AfiI CCNNNNNNNGG 2 cut(s) 452, 1709
AflII CTTAAG 2 cut(s) 740, 1190
AgeI ACCGGT 1 cut(s) 712
AgsI TTSAA 8 cut(s) 282, 710, 1089, 1163, 1204, 1411, 1463, 1668
AhdI GACNNNNNGTC 1 cut(s) 1240
AjiI CACGTC 1 cut(s) 1437
AjnI CCWGG 6 cut(s) 82, 616, 646, 685, 829, 1708
AjuI GAANNNNNNNTTGG 2 cut(s) 651, 683
Alw26I GTCTC 3 cut(s) 68, 404, 759
AlwI GGATC 4 cut(s) 1339, 1363, 1376, 1574
Ama87I CYCGRG 2 cut(s) 450, 783
Aor13HI TCCGGA 1 cut(s) 1679
Aor51HI AGCGCT 1 cut(s) 1458
AoxI GGCC 5 cut(s) 477, 526, 1022, 1115, 1138
ApoI RAATTY 3 cut(s) 361, 672, 1093
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
AsiGI ACCGGT 1 cut(s) 712
AspLEI GCGC 2 cut(s) 1015, 1459
AspS9I GGNCC 4 cut(s) 311, 352, 483, 644
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 7 cut(s) 58, 127, 258, 1037, 1238, 1244, 1586
AvaI CYCGRG 2 cut(s) 450, 783
AvaII GGWCC 4 cut(s) 311, 352, 483, 644
AxyI CCTNAGG 1 cut(s) 111
BaeGI GKGCMC 1 cut(s) 1361
BamHI GGATCC 1 cut(s) 1368
BanI GGYRCC 1 cut(s) 1012
BarI GAAGNNNNNNTAC 2 cut(s) 690, 722
BbsI GAAGAC 2 cut(s) 317, 1284
BccI CCATC 9 cut(s) 95, 218, 649, 1031, 1087, 1208, 1223, 1616, 1711
BceAI ACGGC 1 cut(s) 28
BcgI CGANNNNNNTGC 4 cut(s) 933, 967, 1710, 1744
BciT130I CCWGG 6 cut(s) 84, 618, 648, 687, 831, 1710
BciVI GTATCC 2 cut(s) 90, 305
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 1 cut(s) 481
BcoDI GTCTC 3 cut(s) 68, 404, 759
BfaI CTAG 3 cut(s) 287, 536, 1500
BfmI CTRYAG 5 cut(s) 89, 237, 375, 696, 1476
BfoI RGCGCY 2 cut(s) 1016, 1460
BfrI CTTAAG 2 cut(s) 740, 1190
BfuAI ACCTGC 2 cut(s) 281, 935
BfuI GTATCC 2 cut(s) 90, 305
BglI GCCNNNNNGGC 1 cut(s) 1021
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1710
Bme18I GGWCC 4 cut(s) 311, 352, 483, 644
BmeRI GACNNNNNGTC 1 cut(s) 1240
BmeT110I CYCGRG 2 cut(s) 450, 783
BmgBI CACGTC 1 cut(s) 1437
BmgT120I GGNCC 4 cut(s) 311, 352, 483, 644
BmiI GGNNCC 5 cut(s) 353, 484, 645, 1014, 1370
BmrFI CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1710
BmrI ACTGGG 2 cut(s) 357, 924
BmsI GCATC 6 cut(s) 575, 860, 988, 1294, 1516, 1550
BmuI ACTGGG 2 cut(s) 357, 924
BpiI GAAGAC 2 cut(s) 317, 1284
BplI GAGNNNNNCTC 2 cut(s) 1674, 1706
BpmI CTGGAG 2 cut(s) 528, 741
BpuEI CTTGAG 1 cut(s) 1169
BpuMI CCSGG 1 cut(s) 481
BsaBI GATNNNNATC 1 cut(s) 1041
BsaHI GRCGYC 1 cut(s) 1013
BsaJI CCNNGG 3 cut(s) 83, 1708, 1749
BsaWI WCCGGW 3 cut(s) 143, 712, 1679
Bsc4I CCNNNNNNNGG 2 cut(s) 452, 1709
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 3 cut(s) 313, 352, 919
Bse21I CCTNAGG 1 cut(s) 111
Bse3DI GCAATG 2 cut(s) 611, 1470
Bse8I GATNNNNATC 1 cut(s) 1041
BseAI TCCGGA 1 cut(s) 1679
BseBI CCWGG 6 cut(s) 84, 618, 648, 687, 831, 1710
BseDI CCNNGG 3 cut(s) 83, 1708, 1749
BseGI GGATG 6 cut(s) 106, 229, 259, 724, 1003, 1219
BseJI GATNNNNATC 1 cut(s) 1041
BseLI CCNNNNNNNGG 2 cut(s) 452, 1709
BseMI GCAATG 2 cut(s) 611, 1470
BseMII CTCAG 4 cut(s) 42, 125, 1418, 1620
BseNI ACTGG 3 cut(s) 313, 352, 919
BseRI GAGGAG 1 cut(s) 1767
BseSI GKGCMC 1 cut(s) 1361
BseX3I CGGCCG 1 cut(s) 1115
BsgI GTGCAG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 1118
BshFI GGCC 5 cut(s) 479, 528, 1024, 1117, 1140
BshNI GGYRCC 1 cut(s) 1012
BshTI ACCGGT 1 cut(s) 712
BsiEI CGRYCG 1 cut(s) 1118
BsiHKCI CYCGRG 2 cut(s) 450, 783
BsiSI CCGG 5 cut(s) 144, 480, 713, 1118, 1680
BslFI GGGAC 2 cut(s) 365, 496
BslI CCNNNNNNNGG 2 cut(s) 452, 1709
BsmAI GTCTC 3 cut(s) 68, 404, 759
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 2 cut(s) 365, 496
BsmI GAATGC 1 cut(s) 234
BsnI GGCC 5 cut(s) 479, 528, 1024, 1117, 1140
BsoBI CYCGRG 2 cut(s) 450, 783
Bsp1286I GDGCHC 1 cut(s) 1361
Bsp13I TCCGGA 1 cut(s) 1679
Bsp143I GATC 5 cut(s) 703, 973, 1331, 1368, 1579
BspACI CCGC 3 cut(s) 446, 549, 1613
BspANI GGCC 5 cut(s) 479, 528, 1024, 1117, 1140
BspCNI CTCAG 4 cut(s) 43, 124, 1417, 1621
BspHI TCATGA 1 cut(s) 963
BspLI GGNNCC 5 cut(s) 353, 484, 645, 1014, 1370
BspMAI CTGCAG 4 cut(s) 93, 241, 379, 1480
BspMI ACCTGC 2 cut(s) 281, 935
BspPI GGATC 4 cut(s) 1339, 1363, 1376, 1574
BspT107I GGYRCC 1 cut(s) 1012
BspTI CTTAAG 2 cut(s) 740, 1190
BsrDI GCAATG 2 cut(s) 611, 1470
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 3 cut(s) 313, 352, 919
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 3 cut(s) 83, 1708, 1749
BssMI GATC 5 cut(s) 703, 973, 1331, 1368, 1579
BssNAI GTATAC 1 cut(s) 912
BssNI GRCGYC 1 cut(s) 1013
BssT1I CCWWGG 1 cut(s) 1749
Bst1107I GTATAC 1 cut(s) 912
Bst2UI CCWGG 6 cut(s) 84, 618, 648, 687, 831, 1710
Bst4CI ACNGT 7 cut(s) 356, 415, 638, 1238, 1270, 1318, 1549
Bst6I CTCTTC 1 cut(s) 1339
BstACI GRCGYC 1 cut(s) 1013
BstAFI CTTAAG 2 cut(s) 740, 1190
BstC8I GCNNGC 4 cut(s) 132, 201, 384, 526
BstDEI CTNAG 6 cut(s) 51, 111, 870, 1404, 1629, 1638
BstF5I GGATG 6 cut(s) 106, 229, 259, 724, 1003, 1219
BstH2I RGCGCY 2 cut(s) 1016, 1460
BstHHI GCGC 2 cut(s) 1015, 1459
BstKTI GATC 5 cut(s) 706, 976, 1334, 1371, 1582
BstMAI GTCTC 3 cut(s) 68, 404, 759
BstMBI GATC 5 cut(s) 703, 973, 1331, 1368, 1579
BstMCI CGRYCG 1 cut(s) 1118
BstNI CCWGG 6 cut(s) 84, 618, 648, 687, 831, 1710
BstSCI CCNGG 7 cut(s) 82, 479, 616, 646, 685, 829, 1708
BstSFI CTRYAG 5 cut(s) 89, 237, 375, 696, 1476
BstSLI GKGCMC 1 cut(s) 1361
BstV2I GAAGAC 2 cut(s) 317, 1284
BstX2I RGATCY 1 cut(s) 1368
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 1368
BstZ17I GTATAC 1 cut(s) 912
BstZI CGGCCG 1 cut(s) 1115
Bsu36I CCTNAGG 1 cut(s) 111
BsuI GTATCC 2 cut(s) 90, 305
BsuRI GGCC 5 cut(s) 479, 528, 1024, 1117, 1140
BtgZI GCGATG 1 cut(s) 1730
BtrI CACGTC 1 cut(s) 1437
BtsCI GGATG 6 cut(s) 106, 229, 259, 724, 1003, 1219
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 2 cut(s) 903, 1323
BveI ACCTGC 2 cut(s) 281, 935
Cac8I GCNNGC 4 cut(s) 132, 201, 384, 526
CciI TCATGA 1 cut(s) 963
CfoI GCGC 2 cut(s) 1015, 1459
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 4 cut(s) 311, 352, 483, 644
CseI GACGC 1 cut(s) 1716
CsiI ACCWGGT 1 cut(s) 685
Csp6I GTAC 3 cut(s) 94, 866, 1735
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 6 cut(s) 194, 677, 887, 964, 1282, 1645
CviQI GTAC 3 cut(s) 94, 866, 1735
DdeI CTNAG 6 cut(s) 51, 111, 870, 1404, 1629, 1638
DinI GGCGCC 1 cut(s) 1014
DpnI GATC 5 cut(s) 705, 975, 1333, 1370, 1581
DpnII GATC 5 cut(s) 703, 973, 1331, 1368, 1579
DraI TTTAAA 1 cut(s) 360
DriI GACNNNNNGTC 1 cut(s) 1240
EaeI YGGCCR 2 cut(s) 477, 1115
EagI CGGCCG 1 cut(s) 1115
Eam1104I CTCTTC 1 cut(s) 1339
Eam1105I GACNNNNNGTC 1 cut(s) 1240
EarI CTCTTC 1 cut(s) 1339
EclXI CGGCCG 1 cut(s) 1115
Eco130I CCWWGG 1 cut(s) 1749
Eco47I GGWCC 4 cut(s) 311, 352, 483, 644
Eco47III AGCGCT 1 cut(s) 1458
Eco52I CGGCCG 1 cut(s) 1115
Eco57I CTGAAG 5 cut(s) 300, 1473, 1538, 1629, 1680
Eco81I CCTNAGG 1 cut(s) 111
Eco88I CYCGRG 2 cut(s) 450, 783
EcoRI GAATTC 2 cut(s) 672, 1093
EcoRII CCWGG 6 cut(s) 82, 616, 646, 685, 829, 1708
EcoT14I CCWWGG 1 cut(s) 1749
EgeI GGCGCC 1 cut(s) 1014
EheI GGCGCC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 1749
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 6 cut(s) 197, 680, 890, 967, 1285, 1648
FaqI GGGAC 2 cut(s) 365, 496
FatI CATG 6 cut(s) 193, 676, 886, 963, 1281, 1644
FauI CCCGC 1 cut(s) 453
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 2 cut(s) 911, 1634
FokI GGATG 6 cut(s) 113, 236, 266, 731, 1010, 1226
FspBI CTAG 3 cut(s) 287, 536, 1500
GlaI GCGC 2 cut(s) 1014, 1458
GsuI CTGGAG 2 cut(s) 528, 741
HaeII RGCGCY 2 cut(s) 1016, 1460
HaeIII GGCC 5 cut(s) 479, 528, 1024, 1117, 1140
HapII CCGG 5 cut(s) 144, 480, 713, 1118, 1680
HgaI GACGC 1 cut(s) 1716
HhaI GCGC 2 cut(s) 1015, 1459
Hin1I GRCGYC 1 cut(s) 1013
Hin1II CATG 6 cut(s) 197, 680, 890, 967, 1285, 1648
Hin6I GCGC 2 cut(s) 1013, 1457
HinP1I GCGC 2 cut(s) 1013, 1457
HincII GTYRAC 2 cut(s) 1245, 1635
HindII GTYRAC 2 cut(s) 1245, 1635
HindIII AAGCTT 1 cut(s) 1464
HinfI GANTC 5 cut(s) 278, 1060, 1144, 1631, 1774
HpaII CCGG 5 cut(s) 144, 480, 713, 1118, 1680
HphI GGTGA 7 cut(s) 58, 127, 258, 1037, 1238, 1244, 1586
Hpy166II GTNNAC 4 cut(s) 912, 1245, 1321, 1635
Hpy188I TCNGA 9 cut(s) 299, 330, 394, 571, 1099, 1299, 1343, 1515, 1609
Hpy8I GTNNAC 4 cut(s) 912, 1245, 1321, 1635
Hpy99I CGWCG 1 cut(s) 1732
HpyAV CCTTC 6 cut(s) 56, 112, 324, 389, 462, 961
HpyCH4III ACNGT 7 cut(s) 356, 415, 638, 1238, 1270, 1318, 1549
HpyCH4IV ACGT 5 cut(s) 77, 152, 735, 991, 1436
HpyF3I CTNAG 6 cut(s) 51, 111, 870, 1404, 1629, 1638
HpySE526I ACGT 5 cut(s) 77, 152, 735, 991, 1436
Hsp92I GRCGYC 1 cut(s) 1013
Hsp92II CATG 6 cut(s) 197, 680, 890, 967, 1285, 1648
HspAI GCGC 2 cut(s) 1013, 1457
KasI GGCGCC 1 cut(s) 1012
Kpn2I TCCGGA 1 cut(s) 1679
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 5 cut(s) 703, 973, 1331, 1368, 1579
LmnI GCTCC 3 cut(s) 509, 838, 1625
LweI GCATC 6 cut(s) 575, 860, 988, 1294, 1516, 1550
MabI ACCWGGT 1 cut(s) 685
MaeI CTAG 3 cut(s) 287, 536, 1500
MaeII ACGT 5 cut(s) 77, 152, 735, 991, 1436
MaeIII GTNAC 7 cut(s) 46, 304, 398, 625, 1232, 1432, 1585
MalI GATC 5 cut(s) 705, 975, 1333, 1370, 1581
MboI GATC 5 cut(s) 703, 973, 1331, 1368, 1579
MboII GAAGA 5 cut(s) 317, 698, 1289, 1356, 1544
MflI RGATCY 1 cut(s) 1368
MhlI GDGCHC 1 cut(s) 1361
Mly113I GGCGCC 1 cut(s) 1013
MlyI GAGTC 2 cut(s) 1640, 1768
MmeI TCCRAC 2 cut(s) 403, 559
MroI TCCGGA 1 cut(s) 1679
MseI TTAA 6 cut(s) 359, 741, 1128, 1182, 1191, 1565
MspA1I CMGCKG 2 cut(s) 880, 1169
MspCI CTTAAG 2 cut(s) 740, 1190
MspI CCGG 5 cut(s) 144, 480, 713, 1118, 1680
MspR9I CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1710
Mva1269I GAATGC 1 cut(s) 234
MvaI CCWGG 6 cut(s) 84, 618, 648, 687, 831, 1710
NarI GGCGCC 1 cut(s) 1013
NciI CCSGG 1 cut(s) 481
NdeII GATC 5 cut(s) 703, 973, 1331, 1368, 1579
NlaIII CATG 6 cut(s) 197, 680, 890, 967, 1285, 1648
NlaIV GGNNCC 5 cut(s) 353, 484, 645, 1014, 1370
NmuCI GTSAC 5 cut(s) 46, 625, 1232, 1432, 1585
PaeR7I CTCGAG 1 cut(s) 450
PagI TCATGA 1 cut(s) 963
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 234
PfeI GAWTC 3 cut(s) 278, 1060, 1144
PfoI TCCNGGA 2 cut(s) 646, 829
PinAI ACCGGT 1 cut(s) 712
PleI GAGTC 2 cut(s) 1639, 1768
PluTI GGCGCC 1 cut(s) 1016
PpsI GAGTC 2 cut(s) 1639, 1768
Psp6I CCWGG 6 cut(s) 82, 616, 646, 685, 829, 1708
PspGI CCWGG 6 cut(s) 82, 616, 646, 685, 829, 1708
PspN4I GGNNCC 5 cut(s) 353, 484, 645, 1014, 1370
PspPI GGNCC 4 cut(s) 311, 352, 483, 644
PspXI VCTCGAGB 1 cut(s) 450
PstI CTGCAG 4 cut(s) 93, 241, 379, 1480
PsuI RGATCY 1 cut(s) 1368
PvuII CAGCTG 2 cut(s) 880, 1169
RsaI GTAC 3 cut(s) 95, 867, 1736
RsaNI GTAC 3 cut(s) 94, 866, 1735
SalI GTCGAC 1 cut(s) 1633
SaqAI TTAA 6 cut(s) 359, 741, 1128, 1182, 1191, 1565
Sau3AI GATC 5 cut(s) 703, 973, 1331, 1368, 1579
Sau96I GGNCC 4 cut(s) 311, 352, 483, 644
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 2 cut(s) 1640, 1768
ScrFI CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1710
SduI GDGCHC 1 cut(s) 1361
SexAI ACCWGGT 1 cut(s) 685
SfaNI GCATC 6 cut(s) 575, 860, 988, 1294, 1516, 1550
SfcI CTRYAG 5 cut(s) 89, 237, 375, 696, 1476
SfoI GGCGCC 1 cut(s) 1014
Sfr274I CTCGAG 1 cut(s) 450
SinI GGWCC 4 cut(s) 311, 352, 483, 644
SlaI CTCGAG 1 cut(s) 450
SmlI CTYRAG 4 cut(s) 450, 740, 1148, 1190
SmoI CTYRAG 4 cut(s) 450, 740, 1148, 1190
SsiI CCGC 3 cut(s) 446, 549, 1613
SspDI GGCGCC 1 cut(s) 1012
SspMI CTAG 3 cut(s) 287, 536, 1500
StyD4I CCNGG 7 cut(s) 82, 479, 616, 646, 685, 829, 1708
StyI CCWWGG 1 cut(s) 1749
TaaI ACNGT 7 cut(s) 356, 415, 638, 1238, 1270, 1318, 1549
TaiI ACGT 5 cut(s) 80, 155, 738, 994, 1439
TaqI TCGA 6 cut(s) 60, 72, 451, 466, 953, 1634
TatI WGTACW 1 cut(s) 93
TauI GCSGC 2 cut(s) 551, 1616
TfiI GAWTC 3 cut(s) 278, 1060, 1144
Tru1I TTAA 6 cut(s) 359, 741, 1128, 1182, 1191, 1565
Tru9I TTAA 6 cut(s) 359, 741, 1128, 1182, 1191, 1565
TscAI CASTG 2 cut(s) 903, 1323
TseFI GTSAC 5 cut(s) 46, 625, 1232, 1432, 1585
Tsp45I GTSAC 5 cut(s) 46, 625, 1232, 1432, 1585
TspDTI ATGAA 7 cut(s) 46, 485, 665, 980, 1106, 1146, 1298
TspRI CASTG 2 cut(s) 903, 1323
Vha464I CTTAAG 2 cut(s) 740, 1190
VpaK11BI GGWCC 4 cut(s) 311, 352, 483, 644
XapI RAATTY 3 cut(s) 361, 672, 1093
XhoI CTCGAG 1 cut(s) 450
XmiI GTMKAC 2 cut(s) 911, 1634
XspI CTAG 3 cut(s) 287, 536, 1500
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.