pycom06g20890

3,4-dihydroxy-2-butanone kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012302
Physical Location & Seq
Forward (+)
23765411 .. 23770383
4973 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g20890.3

Sequence Viewer

Length: 1788 bp
ATGGCTTTTCACGGCAAGAAGCTCATCAACGACCCCAACGATGTGGTGACTGAGTTCATCGAAGGGCTTGTCGAAACGTATCCTGGGCTGCAGTACTTGGATGGTTTCCCTCAGGTGAAGGTTGTGTTGCGTGCTGATGTTTCCGGTGGGACGTATGACAAAGTTGCGATTATATCAGGAGGTGGAAGTGGTCATGAGCCTGCACACGCTGGATTTGTGGGGGATGGAATGCTAACTGCAGCTATATGTGGGGATGTTTTTGCCTCACCACAAGTTGATTCAATTCTAGCAGGCATCCGAGCTGTGACTGGTCCTTCAGGTTGTCTTCTGATTGTCAAGAATTATACTGGGGACCGTTTAAATTTTGGTTTAGCTGCAGAGCAAGCAAAATCAGAAGGTTACGAAGTAGAGACTGTAATTGTTGGAGATGATTGTGCATTACCGCCGCCTCGAGGAATAGCTGGTCGAAGGGGTTTGGCCGGGACCATTCTTGTTCATAAGATTGCTGGAGCTGCTGCTGCTGCTGGCCTTTCACTAGCTGATGTTGCTGCAGAAGCAAGACGAGCATCTGAAATGGTTGGAACAATGGGTGTTGCACTATCTATTTGCACATTGCCTGGTCGGGTCACACCAGACCGTTTGGGTCCAGGAAAGATGGAACTTGGTCTTGGAATTCATGGAGAACCCGGTGCTGCTGTAGCTGATCTTCAACCGGTGGATGTTATCGTTTCTCACGTTCTTAAGCAGATATTGTCTCCAGAAACTAATTATGTTCCGATTACTCGGGGTTGTAGAGTGGTGCTTATGGTCAATGGGTTAGGTGCAACTCCTGGAATGGAGCTGATGATTGCATCTGGTAAAGCAGTACCTAAGTTGCAGCTGGAACATGGATTGGCTGTGGATAGAGTGTATACTGGATCATTTATGACTTCTCTTGATATGGCAGGTTTTTCAATTTCTGTCATGAAGGCTGATCAAACTATTTTGCAACGTTTGGATGCTGCAACAAAGGCGCCATATTGGCCTGTTGGTGTTGATGGCAATCACCCATCAGCAAAGATTCCTGTTCCATTACCTCCATCTCATTCAATGAATTCTGATGAGCCATTAGGTCGGCCGGCACAGCTTAATGAACAAGGCCAGATTCTTGAGCTGGCCGTTGAAGCAGCTGCAAATGCAATTCAACAACTTAAGGACAATTTGAACGAATGGGATGGCAAAGTAGGTGATGGTGACTGTGGGTCAACAATGTATATAGGTGCAACAACAGTTTTGGAAGACATGAAAAATTATCCTCTGAATGATGCAACTGAAACAGTGAACGAAATTGGATCATCTATCAGAAGAGCAATGGGGGGCACAAGTGGGATCCTGTATGTTATATTTTGTAAGGCAGCCTACACTCGGTTGAAATCAAGCACCCAATCAGTTGTCACGTCAAAAAATTGGGCTGAAGCACTTGAAGCTTCCATTGCTGCAGTCAGTAAATATGGTGGGGCTAGTGCTGGTTATCGGACAATGTTAGATGCCCTTATTCCAGCATCTACAGTTCTTCAGGAGAGGTTAAAAGCTGGTGATGATCGATCTGTGACTGCTTTTTTACTCTCATCTGAAGCGGCATTAGATGGAGCTGAGTCGACTAAGAACATGGAGGCACAGGCTGGTCGTTCAAGTTATATCTCCGGAGACGTACTTGCTTCAGTTCCAGACCCAGGTGCGATGGCTGCAGCGTCATGGTACAGAGCAGCAGCCTTAGCTGTCAAGTACAAAAACCAGACTCCTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00561 ko00680 ko01100 ko01120 ko01200 ko04622 map00051 map00561 map00680 map01100 map01120 map01200 map04622
Pfam Domains
Protein Families
Dak1 Dak2

Protein Analysis

596

Amino Acids

61.88

Weight (kDa)

5.22

Isoelectric Point (pI)

27.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012302)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48430 AT1G48430 AT3G17770
fragaria_vesca FvH4_5g17820
malus_domestica MD06G1234200.v1.1 MD14G1240900.v1.1
prunus_persica Prupe.5G238700_v2.0.a1
pyrus_communis pycom06g20890 pycom14g20240
rosa_chinensis RchiOBHm_Chr7g0177761
rosa_laevigata RLG00000005506
rosa_multiflora Rmu_sc0006214.1_g000015
rosa_roxburghii Rroxscaffold_3G00275580
rosa_rugosa Rorug06G0409300
rosa_samantha Rh7AG008900 Rh7BG009000 Rh7CG009200 Rh7DG009000
rosa_wichuraiana Rw7G000730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 935
AccB1I GGYRCC 1 cut(s) 1012
AccI GTMKAC 2 cut(s) 911, 1637
AccIII TCCGGA 1 cut(s) 1682
AciI CCGC 3 cut(s) 443, 446, 1616
AclI AACGTT 1 cut(s) 991
AclWI GGATC 4 cut(s) 925, 1339, 1363, 1376
AcoI YGGCCR 3 cut(s) 477, 1115, 1155
AcsI RAATTY 3 cut(s) 361, 672, 1093
AcuI CTGAAG 5 cut(s) 300, 1473, 1538, 1632, 1683
AcyI GRCGYC 1 cut(s) 1013
AfaI GTAC 5 cut(s) 95, 867, 1692, 1739, 1766
AfiI CCNNNNNNNGG 3 cut(s) 452, 1404, 1712
AflII CTTAAG 2 cut(s) 740, 1190
AgeI ACCGGT 1 cut(s) 712
AhdI GACNNNNNGTC 1 cut(s) 1240
AjiI CACGTC 1 cut(s) 1437
AjnI CCWGG 5 cut(s) 82, 616, 646, 829, 1711
AjuI GAANNNNNNNTTGG 2 cut(s) 651, 683
Alw26I GTCTC 3 cut(s) 404, 759, 1680
AlwI GGATC 4 cut(s) 925, 1339, 1363, 1376
Ama87I CYCGRG 2 cut(s) 450, 783
Aor13HI TCCGGA 1 cut(s) 1682
AoxI GGCC 6 cut(s) 477, 526, 1022, 1115, 1138, 1155
ApoI RAATTY 3 cut(s) 361, 672, 1093
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
AsiGI ACCGGT 1 cut(s) 712
AspLEI GCGC 1 cut(s) 1015
AspS9I GGNCC 4 cut(s) 311, 352, 483, 644
AsuC2I CCSGG 2 cut(s) 481, 687
AsuHPI GGTGA 7 cut(s) 58, 127, 258, 1037, 1238, 1244, 1586
AvaI CYCGRG 2 cut(s) 450, 783
AvaII GGWCC 4 cut(s) 311, 352, 483, 644
AxyI CCTNAGG 1 cut(s) 111
BaeGI GKGCMC 1 cut(s) 1361
BamHI GGATCC 1 cut(s) 1368
BanI GGYRCC 1 cut(s) 1012
BarI GAAGNNNNNNTAC 2 cut(s) 690, 722
BbsI GAAGAC 2 cut(s) 317, 1284
BceAI ACGGC 2 cut(s) 28, 1142
BciT130I CCWGG 5 cut(s) 84, 618, 648, 831, 1713
BciVI GTATCC 1 cut(s) 90
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 2 cut(s) 481, 687
BcoDI GTCTC 3 cut(s) 404, 759, 1680
BfaI CTAG 3 cut(s) 287, 536, 1500
BfmI CTRYAG 8 cut(s) 89, 237, 375, 549, 696, 1476, 1545, 1725
BfoI RGCGCY 1 cut(s) 1016
BfrI CTTAAG 2 cut(s) 740, 1190
BfuAI ACCTGC 1 cut(s) 935
BfuI GTATCC 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 1021
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1713
Bme18I GGWCC 4 cut(s) 311, 352, 483, 644
BmeRI GACNNNNNGTC 1 cut(s) 1240
BmeT110I CYCGRG 2 cut(s) 450, 783
BmgBI CACGTC 1 cut(s) 1437
BmgT120I GGNCC 4 cut(s) 311, 352, 483, 644
BmiI GGNNCC 5 cut(s) 353, 484, 645, 1014, 1370
BmrFI CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1713
BmrI ACTGGG 1 cut(s) 357
BmsI GCATC 7 cut(s) 303, 575, 860, 988, 1294, 1516, 1550
BmuI ACTGGG 1 cut(s) 357
BpiI GAAGAC 2 cut(s) 317, 1284
BpmI CTGGAG 2 cut(s) 528, 741
Bpu10I CCTNAGC 1 cut(s) 1753
BpuEI CTTGAG 1 cut(s) 1169
BpuMI CCSGG 2 cut(s) 481, 687
Bsa29I ATCGAT 1 cut(s) 1582
BsaBI GATNNNNATC 1 cut(s) 1041
BsaHI GRCGYC 1 cut(s) 1013
BsaJI CCNNGG 2 cut(s) 83, 1711
BsaWI WCCGGW 3 cut(s) 143, 712, 1682
Bsc4I CCNNNNNNNGG 3 cut(s) 452, 1404, 1712
Bse118I RCCGGY 2 cut(s) 712, 1117
Bse1I ACTGG 3 cut(s) 313, 352, 919
Bse21I CCTNAGG 1 cut(s) 111
Bse3DI GCAATG 3 cut(s) 611, 1356, 1470
Bse8I GATNNNNATC 1 cut(s) 1041
BseAI TCCGGA 1 cut(s) 1682
BseBI CCWGG 5 cut(s) 84, 618, 648, 831, 1713
BseCI ATCGAT 1 cut(s) 1582
BseDI CCNNGG 2 cut(s) 83, 1711
BseGI GGATG 7 cut(s) 106, 229, 259, 294, 724, 1003, 1219
BseJI GATNNNNATC 1 cut(s) 1041
BseLI CCNNNNNNNGG 3 cut(s) 452, 1404, 1712
BseMI GCAATG 3 cut(s) 611, 1356, 1470
BseMII CTCAG 3 cut(s) 42, 125, 1623
BseNI ACTGG 3 cut(s) 313, 352, 919
BseRI GAGGAG 1 cut(s) 1770
BseSI GKGCMC 1 cut(s) 1361
BseX3I CGGCCG 1 cut(s) 1115
BsgI GTGCAG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 1118
BshFI GGCC 6 cut(s) 479, 528, 1024, 1117, 1140, 1157
BshNI GGYRCC 1 cut(s) 1012
BshTI ACCGGT 1 cut(s) 712
BshVI ATCGAT 1 cut(s) 1582
BsiEI CGRYCG 1 cut(s) 1118
BsiHKCI CYCGRG 2 cut(s) 450, 783
BsiSI CCGG 6 cut(s) 144, 480, 687, 713, 1118, 1683
BslFI GGGAC 3 cut(s) 163, 365, 496
BslI CCNNNNNNNGG 3 cut(s) 452, 1404, 1712
BsmAI GTCTC 3 cut(s) 404, 759, 1680
BsmBI CGTCTC 1 cut(s) 1680
BsmFI GGGAC 3 cut(s) 163, 365, 496
BsmI GAATGC 1 cut(s) 234
BsnI GGCC 6 cut(s) 479, 528, 1024, 1117, 1140, 1157
BsoBI CYCGRG 2 cut(s) 450, 783
Bsp1286I GDGCHC 1 cut(s) 1361
Bsp13I TCCGGA 1 cut(s) 1682
Bsp143I GATC 7 cut(s) 703, 917, 973, 1331, 1368, 1579, 1583
BspACI CCGC 3 cut(s) 443, 446, 1616
BspANI GGCC 6 cut(s) 479, 528, 1024, 1117, 1140, 1157
BspCNI CTCAG 3 cut(s) 43, 124, 1624
BspDI ATCGAT 1 cut(s) 1582
BspEI TCCGGA 1 cut(s) 1682
BspHI TCATGA 2 cut(s) 193, 963
BspLI GGNNCC 5 cut(s) 353, 484, 645, 1014, 1370
BspMAI CTGCAG 6 cut(s) 93, 241, 379, 553, 1480, 1729
BspMI ACCTGC 1 cut(s) 935
BspPI GGATC 4 cut(s) 925, 1339, 1363, 1376
BspQI GCTCTTC 1 cut(s) 1339
BspT107I GGYRCC 1 cut(s) 1012
BspTI CTTAAG 2 cut(s) 740, 1190
BsrDI GCAATG 3 cut(s) 611, 1356, 1470
BsrFI RCCGGY 2 cut(s) 712, 1117
BsrI ACTGG 3 cut(s) 313, 352, 919
BssAI RCCGGY 2 cut(s) 712, 1117
BssECI CCNNGG 2 cut(s) 83, 1711
BssMI GATC 7 cut(s) 703, 917, 973, 1331, 1368, 1579, 1583
BssNAI GTATAC 1 cut(s) 912
BssNI GRCGYC 1 cut(s) 1013
Bst1107I GTATAC 1 cut(s) 912
Bst2UI CCWGG 5 cut(s) 84, 618, 648, 831, 1713
Bst4CI ACNGT 7 cut(s) 356, 415, 638, 1238, 1270, 1318, 1549
Bst6I CTCTTC 1 cut(s) 1339
BstACI GRCGYC 1 cut(s) 1013
BstAFI CTTAAG 2 cut(s) 740, 1190
BstC8I GCNNGC 7 cut(s) 132, 201, 292, 384, 526, 1119, 1155
BstDEI CTNAG 6 cut(s) 51, 111, 870, 1632, 1641, 1753
BstF5I GGATG 7 cut(s) 106, 229, 259, 294, 724, 1003, 1219
BstH2I RGCGCY 1 cut(s) 1016
BstHHI GCGC 1 cut(s) 1015
BstKTI GATC 7 cut(s) 706, 920, 976, 1334, 1371, 1582, 1586
BstMAI GTCTC 3 cut(s) 404, 759, 1680
BstMBI GATC 7 cut(s) 703, 917, 973, 1331, 1368, 1579, 1583
BstMCI CGRYCG 1 cut(s) 1118
BstNI CCWGG 5 cut(s) 84, 618, 648, 831, 1713
BstSCI CCNGG 7 cut(s) 82, 479, 616, 646, 685, 829, 1711
BstSFI CTRYAG 8 cut(s) 89, 237, 375, 549, 696, 1476, 1545, 1725
BstSLI GKGCMC 1 cut(s) 1361
BstV2I GAAGAC 2 cut(s) 317, 1284
BstX2I RGATCY 1 cut(s) 1368
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 1368
BstZ17I GTATAC 1 cut(s) 912
BstZI CGGCCG 1 cut(s) 1115
Bsu15I ATCGAT 1 cut(s) 1582
Bsu36I CCTNAGG 1 cut(s) 111
BsuI GTATCC 1 cut(s) 90
BsuRI GGCC 6 cut(s) 479, 528, 1024, 1117, 1140, 1157
BsuTUI ATCGAT 1 cut(s) 1582
BtgZI GCGATG 1 cut(s) 1733
BtrI CACGTC 1 cut(s) 1437
BtsCI GGATG 7 cut(s) 106, 229, 259, 294, 724, 1003, 1219
BtsIMutI CAGTG 1 cut(s) 1323
BveI ACCTGC 1 cut(s) 935
Cac8I GCNNGC 7 cut(s) 132, 201, 292, 384, 526, 1119, 1155
CciI TCATGA 2 cut(s) 193, 963
CfoI GCGC 1 cut(s) 1015
Cfr10I RCCGGY 2 cut(s) 712, 1117
Cfr13I GGNCC 4 cut(s) 311, 352, 483, 644
ClaI ATCGAT 1 cut(s) 1582
CseI GACGC 1 cut(s) 1719
Csp6I GTAC 5 cut(s) 94, 866, 1691, 1738, 1765
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 7 cut(s) 194, 677, 887, 964, 1282, 1648, 1734
CviQI GTAC 5 cut(s) 94, 866, 1691, 1738, 1765
DdeI CTNAG 6 cut(s) 51, 111, 870, 1632, 1641, 1753
DinI GGCGCC 1 cut(s) 1014
DpnI GATC 7 cut(s) 705, 919, 975, 1333, 1370, 1581, 1585
DpnII GATC 7 cut(s) 703, 917, 973, 1331, 1368, 1579, 1583
DraI TTTAAA 1 cut(s) 360
DriI GACNNNNNGTC 1 cut(s) 1240
EaeI YGGCCR 3 cut(s) 477, 1115, 1155
EagI CGGCCG 1 cut(s) 1115
Eam1104I CTCTTC 1 cut(s) 1339
Eam1105I GACNNNNNGTC 1 cut(s) 1240
EarI CTCTTC 1 cut(s) 1339
EclXI CGGCCG 1 cut(s) 1115
Eco47I GGWCC 4 cut(s) 311, 352, 483, 644
Eco52I CGGCCG 1 cut(s) 1115
Eco57I CTGAAG 5 cut(s) 300, 1473, 1538, 1632, 1683
Eco81I CCTNAGG 1 cut(s) 111
Eco88I CYCGRG 2 cut(s) 450, 783
EcoRI GAATTC 2 cut(s) 672, 1093
EcoRII CCWGG 5 cut(s) 82, 616, 646, 829, 1711
EgeI GGCGCC 1 cut(s) 1014
EheI GGCGCC 1 cut(s) 1014
Esp3I CGTCTC 1 cut(s) 1680
FaeI CATG 7 cut(s) 197, 680, 890, 967, 1285, 1651, 1737
FaqI GGGAC 3 cut(s) 163, 365, 496
FatI CATG 7 cut(s) 193, 676, 886, 963, 1281, 1647, 1733
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 2 cut(s) 911, 1637
FokI GGATG 7 cut(s) 113, 236, 266, 281, 731, 1010, 1226
FspBI CTAG 3 cut(s) 287, 536, 1500
GlaI GCGC 1 cut(s) 1014
GsuI CTGGAG 2 cut(s) 528, 741
HaeII RGCGCY 1 cut(s) 1016
HaeIII GGCC 6 cut(s) 479, 528, 1024, 1117, 1140, 1157
HapII CCGG 6 cut(s) 144, 480, 687, 713, 1118, 1683
HgaI GACGC 1 cut(s) 1719
HhaI GCGC 1 cut(s) 1015
Hin1I GRCGYC 1 cut(s) 1013
Hin1II CATG 7 cut(s) 197, 680, 890, 967, 1285, 1651, 1737
Hin6I GCGC 1 cut(s) 1013
HinP1I GCGC 1 cut(s) 1013
HincII GTYRAC 2 cut(s) 1245, 1638
HindII GTYRAC 2 cut(s) 1245, 1638
HindIII AAGCTT 1 cut(s) 1464
HinfI GANTC 5 cut(s) 278, 1060, 1144, 1634, 1777
HpaII CCGG 6 cut(s) 144, 480, 687, 713, 1118, 1683
HphI GGTGA 7 cut(s) 58, 127, 258, 1037, 1238, 1244, 1586
Hpy166II GTNNAC 4 cut(s) 912, 1245, 1321, 1638
Hpy8I GTNNAC 4 cut(s) 912, 1245, 1321, 1638
HpyAV CCTTC 6 cut(s) 56, 112, 324, 389, 462, 961
HpyCH4III ACNGT 7 cut(s) 356, 415, 638, 1238, 1270, 1318, 1549
HpyCH4IV ACGT 6 cut(s) 77, 152, 735, 991, 1436, 1689
HpyF3I CTNAG 6 cut(s) 51, 111, 870, 1632, 1641, 1753
HpySE526I ACGT 6 cut(s) 77, 152, 735, 991, 1436, 1689
Hsp92I GRCGYC 1 cut(s) 1013
Hsp92II CATG 7 cut(s) 197, 680, 890, 967, 1285, 1651, 1737
HspAI GCGC 1 cut(s) 1013
KasI GGCGCC 1 cut(s) 1012
Kpn2I TCCGGA 1 cut(s) 1682
KroI GCCGGC 1 cut(s) 1117
KroNI GCCGGC 1 cut(s) 1119
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 7 cut(s) 703, 917, 973, 1331, 1368, 1579, 1583
LguI GCTCTTC 1 cut(s) 1339
LmnI GCTCC 3 cut(s) 509, 838, 1628
LweI GCATC 7 cut(s) 303, 575, 860, 988, 1294, 1516, 1550
MaeI CTAG 3 cut(s) 287, 536, 1500
MaeII ACGT 6 cut(s) 77, 152, 735, 991, 1436, 1689
MaeIII GTNAC 7 cut(s) 46, 304, 398, 625, 1232, 1432, 1588
MalI GATC 7 cut(s) 705, 919, 975, 1333, 1370, 1581, 1585
MboI GATC 7 cut(s) 703, 917, 973, 1331, 1368, 1579, 1583
MboII GAAGA 5 cut(s) 317, 698, 1289, 1356, 1544
MflI RGATCY 1 cut(s) 1368
MhlI GDGCHC 1 cut(s) 1361
Mly113I GGCGCC 1 cut(s) 1013
MlyI GAGTC 2 cut(s) 1643, 1771
MmeI TCCRAC 2 cut(s) 403, 559
MnlI CCTC 9 cut(s) 120, 173, 274, 446, 459, 1086, 1305, 1554, 1645
MroI TCCGGA 1 cut(s) 1682
MroNI GCCGGC 1 cut(s) 1117
MseI TTAA 5 cut(s) 359, 741, 1128, 1191, 1565
MspA1I CMGCKG 2 cut(s) 880, 1169
MspCI CTTAAG 2 cut(s) 740, 1190
MspI CCGG 6 cut(s) 144, 480, 687, 713, 1118, 1683
MspR9I CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1713
Mva1269I GAATGC 1 cut(s) 234
MvaI CCWGG 5 cut(s) 84, 618, 648, 831, 1713
NaeI GCCGGC 1 cut(s) 1119
NarI GGCGCC 1 cut(s) 1013
NciI CCSGG 2 cut(s) 481, 687
NdeII GATC 7 cut(s) 703, 917, 973, 1331, 1368, 1579, 1583
NgoMIV GCCGGC 1 cut(s) 1117
NlaIII CATG 7 cut(s) 197, 680, 890, 967, 1285, 1651, 1737
NlaIV GGNNCC 5 cut(s) 353, 484, 645, 1014, 1370
NmuCI GTSAC 6 cut(s) 46, 304, 625, 1232, 1432, 1588
PaeR7I CTCGAG 1 cut(s) 450
PagI TCATGA 2 cut(s) 193, 963
PciSI GCTCTTC 1 cut(s) 1339
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 732
PctI GAATGC 1 cut(s) 234
PdiI GCCGGC 1 cut(s) 1119
PfeI GAWTC 3 cut(s) 278, 1060, 1144
PfoI TCCNGGA 2 cut(s) 646, 829
PinAI ACCGGT 1 cut(s) 712
PleI GAGTC 2 cut(s) 1642, 1771
PluTI GGCGCC 1 cut(s) 1016
PpsI GAGTC 2 cut(s) 1642, 1771
Psp1406I AACGTT 1 cut(s) 991
Psp6I CCWGG 5 cut(s) 82, 616, 646, 829, 1711
PspGI CCWGG 5 cut(s) 82, 616, 646, 829, 1711
PspN4I GGNNCC 5 cut(s) 353, 484, 645, 1014, 1370
PspPI GGNCC 4 cut(s) 311, 352, 483, 644
PspXI VCTCGAGB 1 cut(s) 450
PstI CTGCAG 6 cut(s) 93, 241, 379, 553, 1480, 1729
PsuI RGATCY 1 cut(s) 1368
PvuII CAGCTG 2 cut(s) 880, 1169
RsaI GTAC 5 cut(s) 95, 867, 1692, 1739, 1766
RsaNI GTAC 5 cut(s) 94, 866, 1691, 1738, 1765
SalI GTCGAC 1 cut(s) 1636
SapI GCTCTTC 1 cut(s) 1339
SaqAI TTAA 5 cut(s) 359, 741, 1128, 1191, 1565
Sau3AI GATC 7 cut(s) 703, 917, 973, 1331, 1368, 1579, 1583
Sau96I GGNCC 4 cut(s) 311, 352, 483, 644
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 2 cut(s) 1643, 1771
ScrFI CCNGG 7 cut(s) 84, 481, 618, 648, 687, 831, 1713
SduI GDGCHC 1 cut(s) 1361
SfaNI GCATC 7 cut(s) 303, 575, 860, 988, 1294, 1516, 1550
SfcI CTRYAG 8 cut(s) 89, 237, 375, 549, 696, 1476, 1545, 1725
SfoI GGCGCC 1 cut(s) 1014
Sfr274I CTCGAG 1 cut(s) 450
SinI GGWCC 4 cut(s) 311, 352, 483, 644
SlaI CTCGAG 1 cut(s) 450
SmlI CTYRAG 4 cut(s) 450, 740, 1148, 1190
SmoI CTYRAG 4 cut(s) 450, 740, 1148, 1190
SsiI CCGC 3 cut(s) 443, 446, 1616
SspDI GGCGCC 1 cut(s) 1012
SspMI CTAG 3 cut(s) 287, 536, 1500
StyD4I CCNGG 7 cut(s) 82, 479, 616, 646, 685, 829, 1711
TaaI ACNGT 7 cut(s) 356, 415, 638, 1238, 1270, 1318, 1549
TaiI ACGT 6 cut(s) 80, 155, 738, 994, 1439, 1692
TaqI TCGA 6 cut(s) 60, 72, 451, 466, 1582, 1637
TatI WGTACW 2 cut(s) 93, 1764
TauI GCSGC 2 cut(s) 448, 1619
TfiI GAWTC 3 cut(s) 278, 1060, 1144
Tru1I TTAA 5 cut(s) 359, 741, 1128, 1191, 1565
Tru9I TTAA 5 cut(s) 359, 741, 1128, 1191, 1565
TscAI CASTG 1 cut(s) 1323
TseFI GTSAC 6 cut(s) 46, 304, 625, 1232, 1432, 1588
Tsp45I GTSAC 6 cut(s) 46, 304, 625, 1232, 1432, 1588
TspDTI ATGAA 7 cut(s) 46, 485, 665, 980, 1106, 1146, 1298
TspRI CASTG 1 cut(s) 1323
Vha464I CTTAAG 2 cut(s) 740, 1190
VpaK11BI GGWCC 4 cut(s) 311, 352, 483, 644
XapI RAATTY 3 cut(s) 361, 672, 1093
XhoI CTCGAG 1 cut(s) 450
XmiI GTMKAC 2 cut(s) 911, 1637
XspI CTAG 3 cut(s) 287, 536, 1500
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.