FvH4_5g19881

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017669
Physical Location & Seq
Reverse (-)
11697980 .. 11698880
901 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19881.t1

Sequence Viewer

Length: 765 bp
ATGTCGATAGATCTCAGTTCTGACTCAACAATAGAGATGGTGGTGCAAGATCGAAACTTGCCTGTGACCTCTCCATCTCATCAAAATAGACGTGCGGGACGCAGCCAACAAGGTTCTTCCTCCTCTAAACTCAAAGGCTTGGTGCCACTGAAAGGTGGTAAGTGGGGGGCAAGGATATCCTATAACTATAAGCGATACAGCCTCGGGACACATGAAACAGAGATCGAGGCAGCTATAGCATATGATAGAGCTGCGCTCAAGATTCCGAGAGCTCATTCCCTCAACTTCCACCGGAGTAATTACACTATCGAGGAGAGCACATTCCAAAGTCAGCACTCAATTGAGGATATTCTGAGTATGTTGCATGATAAGACATACACATTCATGCTTAGGAATTTTATTTTTCAGTATTCATCTAGTGTTAACTATCATGCAGAAATCTTCATGAAGGAACAGGGGATTGTGTATGAATGTGTCCTTCAGAAGGAACTGACCCTTATGGATGTCACTCACAAGTGCTTCCTGGTTCAAGGGGATTATGCATCACTGCATTTCCCCACAAAAGAACATGAATGCCTGAATAGCACTGCCACTCTCTGGGATATTCATGGCCTCTCATGGACAAAGTTTTACTTAATCAATGTGCTGAAAACTGCTGGTGAAAGCTCTGTTGGAGGAAGCTTGAACAAGGAGTTGGAAGCCAATCGCTCGGAAGAGGAGAAAAAGAGAGGGGTTAAGCTATTTGGTGTTCAGATTAGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

255

Amino Acids

28.81

Weight (kDa)

7.77

Isoelectric Point (pI)

58.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017669)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19881 FvH4_6g29430
prunus_persica Prupe.5G061400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204681
rosa_laevigata RLG00000003438
rosa_multiflora Rmu_sc0007104.1_g000008
rosa_rugosa Rorug07G0085600
rosa_samantha Rh7AG215500 Rh7BG212700 Rh7CG228600 Rh7DG223200
rosa_wichuraiana Rw7G018680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 142
AccB7I CCANNNNNTGG 1 cut(s) 597
AciI CCGC 1 cut(s) 95
AcsI RAATTY 1 cut(s) 394
AcuI CTGAAG 1 cut(s) 464
AfiI CCNNNNNNNGG 3 cut(s) 152, 484, 597
AgsI TTSAA 2 cut(s) 530, 685
AjiI CACGTC 1 cut(s) 92
AjnI CCWGG 1 cut(s) 522
AjuI GAANNNNNNNTTGG 4 cut(s) 654, 677, 686, 709
AluBI AGCT 6 cut(s) 233, 251, 272, 666, 681, 739
AluI AGCT 6 cut(s) 233, 251, 272, 666, 681, 739
Alw21I GWGCWC 2 cut(s) 274, 320
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 1 cut(s) 610
ApeKI GCWGC 3 cut(s) 102, 230, 251
ApoI RAATTY 1 cut(s) 394
AspLEI GCGC 1 cut(s) 256
AsuHPI GGTGA 1 cut(s) 671
AvaI CYCGRG 1 cut(s) 203
BanI GGYRCC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 274, 320
BbvI GCAGC 3 cut(s) 114, 238, 242
BccI CCATC 2 cut(s) 31, 82
BciT130I CCWGG 1 cut(s) 524
BfaI CTAG 1 cut(s) 417
BfmI CTRYAG 1 cut(s) 234
BglII AGATCT 1 cut(s) 10
BisI GCNGC 3 cut(s) 103, 231, 252
BlsI GCNGC 3 cut(s) 104, 232, 253
Bme1390I CCNGG 1 cut(s) 524
BmeT110I CYCGRG 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 92
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 1 cut(s) 551
BplI GAGNNNNNCTC 2 cut(s) 240, 272
Bpu10I CCTNAGC 1 cut(s) 389
BpuEI CTTGAG 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 202
BsaWI WCCGGW 1 cut(s) 291
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 484, 597
BseBI CCWGG 1 cut(s) 524
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 1 cut(s) 508
BseLI CCNNNNNNNGG 3 cut(s) 152, 484, 597
BseMII CTCAG 2 cut(s) 28, 344
BseRI GAGGAG 3 cut(s) 112, 326, 731
BseXI GCAGC 3 cut(s) 114, 238, 242
BshFI GGCC 1 cut(s) 612
BshNI GGYRCC 1 cut(s) 142
BsiHKAI GWGCWC 2 cut(s) 274, 320
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 2 cut(s) 111, 220
BslI CCNNNNNNNGG 3 cut(s) 152, 484, 597
BsmFI GGGAC 2 cut(s) 111, 220
BsmI GAATGC 1 cut(s) 578
BsnI GGCC 1 cut(s) 612
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 274, 320
Bsp143I GATC 3 cut(s) 10, 49, 222
BspACI CCGC 1 cut(s) 95
BspANI GGCC 1 cut(s) 612
BspCNI CTCAG 2 cut(s) 27, 345
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 144
BspT107I GGYRCC 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 3 cut(s) 10, 49, 222
Bst2UI CCWGG 1 cut(s) 524
Bst6I CTCTTC 1 cut(s) 708
BstDEI CTNAG 3 cut(s) 14, 353, 389
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 1 cut(s) 508
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 3 cut(s) 13, 52, 225
BstMBI GATC 3 cut(s) 10, 49, 222
BstMWI GCNNNNNNNGC 2 cut(s) 236, 582
BstNI CCWGG 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 522
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 3 cut(s) 114, 238, 242
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
BsuRI GGCC 1 cut(s) 612
BtrI CACGTC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 508
BtsI GCAGTG 2 cut(s) 545, 585
BtsIMutI CAGTG 3 cut(s) 146, 545, 585
CciI TCATGA 1 cut(s) 444
CfoI GCGC 1 cut(s) 256
CseI GACGC 1 cut(s) 108
CviAII CATG 8 cut(s) 212, 365, 385, 431, 445, 569, 608, 618
DdeI CTNAG 3 cut(s) 14, 353, 389
DpnI GATC 3 cut(s) 12, 51, 224
DpnII GATC 3 cut(s) 10, 49, 222
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
Ecl136II GAGCTC 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 274
Eco32I GATATC 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 464
Eco88I CYCGRG 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 272
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 522
EcoRV GATATC 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 274
FaeI CATG 8 cut(s) 215, 368, 388, 434, 448, 572, 611, 621
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 2 cut(s) 111, 220
FatI CATG 8 cut(s) 211, 364, 384, 430, 444, 568, 607, 617
FauI CCCGC 1 cut(s) 88
FauNDI CATATG 1 cut(s) 241
Fnu4HI GCNGC 3 cut(s) 103, 231, 252
FokI GGATG 1 cut(s) 515
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 3 cut(s) 103, 231, 252
FspBI CTAG 1 cut(s) 417
GlaI GCGC 1 cut(s) 255
GluI GCNGC 3 cut(s) 103, 231, 252
HaeIII GGCC 1 cut(s) 612
HapII CCGG 1 cut(s) 292
HgaI GACGC 1 cut(s) 108
HhaI GCGC 1 cut(s) 256
Hin1II CATG 8 cut(s) 215, 368, 388, 434, 448, 572, 611, 621
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 679
HinfI GANTC 2 cut(s) 23, 262
HpaI GTTAAC 1 cut(s) 424
HpaII CCGG 1 cut(s) 292
HphI GGTGA 1 cut(s) 671
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 6 cut(s) 22, 267, 354, 483, 712, 753
Hpy188III TCNNGA 3 cut(s) 205, 259, 445
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 3 cut(s) 442, 478, 488
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 5 cut(s) 46, 364, 434, 542, 550
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 582
HpyF3I CTNAG 3 cut(s) 14, 353, 389
HpySE526I ACGT 1 cut(s) 91
Hsp92II CATG 8 cut(s) 215, 368, 388, 434, 448, 572, 611, 621
HspAI GCGC 1 cut(s) 254
KspAI GTTAAC 1 cut(s) 424
Kzo9I GATC 3 cut(s) 10, 49, 222
LpnPI CCDG 8 cut(s) 75, 305, 440, 509, 536, 583, 590, 642
Lsp1109I GCAGC 3 cut(s) 114, 238, 242
LweI GCATC 1 cut(s) 551
MaeI CTAG 1 cut(s) 417
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 2 cut(s) 64, 505
MalI GATC 3 cut(s) 12, 51, 224
MboI GATC 3 cut(s) 10, 49, 222
MboII GAAGA 3 cut(s) 108, 433, 725
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 2 cut(s) 274, 320
MluCI AATT 3 cut(s) 298, 339, 394
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 2 cut(s) 652, 675
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 4 cut(s) 423, 635, 735, 763
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 1 cut(s) 524
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 578
MvaI CCWGG 1 cut(s) 524
MwoI GCNNNNNNNGC 2 cut(s) 236, 582
NdeI CATATG 1 cut(s) 241
NdeII GATC 3 cut(s) 10, 49, 222
NlaIII CATG 8 cut(s) 215, 368, 388, 434, 448, 572, 611, 621
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 2 cut(s) 64, 505
NsiI ATGCAT 1 cut(s) 544
PagI TCATGA 1 cut(s) 444
PctI GAATGC 1 cut(s) 578
PfeI GAWTC 1 cut(s) 262
PflMI CCANNNNNTGG 1 cut(s) 597
PkrI GCNGC 3 cut(s) 104, 232, 253
PleI GAGTC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 17
Psp124BI GAGCTC 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 522
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 1 cut(s) 144
PsuI RGATCY 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 383
SacI GAGCTC 1 cut(s) 274
SaqAI TTAA 4 cut(s) 423, 635, 735, 763
SatI GCNGC 3 cut(s) 103, 231, 252
Sau3AI GATC 3 cut(s) 10, 49, 222
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 1 cut(s) 524
SduI GDGCHC 2 cut(s) 274, 320
SfaNI GCATC 1 cut(s) 551
SfcI CTRYAG 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 3 cut(s) 298, 339, 394
SsiI CCGC 1 cut(s) 95
SspMI CTAG 1 cut(s) 417
SstI GAGCTC 1 cut(s) 274
StyD4I CCNGG 1 cut(s) 522
TaiI ACGT 1 cut(s) 94
TaqI TCGA 4 cut(s) 5, 52, 225, 309
TasI AATT 3 cut(s) 298, 339, 394
TfiI GAWTC 1 cut(s) 262
Tru1I TTAA 4 cut(s) 423, 635, 735, 763
Tru9I TTAA 4 cut(s) 423, 635, 735, 763
TscAI CASTG 3 cut(s) 153, 552, 592
TseFI GTSAC 2 cut(s) 64, 505
TseI GCWGC 3 cut(s) 102, 230, 251
Tsp45I GTSAC 2 cut(s) 64, 505
TspDTI ATGAA 8 cut(s) 228, 373, 402, 433, 461, 483, 585, 596
TspRI CASTG 3 cut(s) 153, 552, 592
Van91I CCANNNNNTGG 1 cut(s) 597
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 1 cut(s) 394
XspI CTAG 1 cut(s) 417
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.