Rh7DG223200

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0017669
Physical Location & Seq
Reverse (-)
21713043 .. 21713981
939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG223200.1

Sequence Viewer

Length: 939 bp
ATGTCAATGGATGTCAGTTCTGACTCAACAATAGAGATGGTGGTGCAAGATCAAAACCTGCCTCCGACTTCTCCATCTCATCAGAATAGATGTACGAGAAGCAGTCAACAAGGTTCTTCCTCCTCTAAACTCAAAGGAGTGATACCACTGAAAGGTGGTATGTGGGGGGCAAGGATCACCTATAACTACAAGCGATACAGGCTTGGGACATATGAAACAGAGATTGAGGCAGCTAAAGCATATGATAGAGCTGCAGTCAAGATGCCGCGAGCTCATTCCCTAAACTTCCACCGGAGTAATTACAGTGTAGAAGAGAGCGCTTTCCAAAGTCAGTACTCAATTGAGGAAGTTCTGAGGATGTTGCAGGATAAAACGTACTCATCGATGCTTCAGAATTTTATTTTGAATCATTCAACTTGTGTTGGATTTCATGCAGAAGCCTTCATGAAGGAACAAGAGATTTCATATGACTTTCTCTTTAAGAAGGAACTGAGTCATATGGATGTCACTCACAAGTGCTTCCTGGTTCAAGGGGATTATGCATCACTACATTCCCCCCCAATAGAAGATGAAGGCTGGAATAGCTTTGCCACTTTGTATGATATACATCGCCGCTCATGGACATTTGGATGTTCATATTGGCAGAGTACACAAAGCTTTATTTTTACTAGTGGCTGGGGAGACTTTCTCGAGATGTATAACCTGAAGGAGAAAGACAAAATAATAATTTACAGATGCCGGTACCAAGGGGATGCTGTCAGGAGAAAATTTCACTTGATTAATGTGCTGAGAAGTACTGCAGAAAGTTCTGTTGTTGGTATGACTACAGAGCAAGATGTCGGTTTGAGAGGAAGCTTGAACAAGGAGTTGGAGGCTGATTGCACTAGTGAGGTGAAAAAGAGAGGGTTTAAGCTCTTTGGTATTGAGATTAGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

312

Amino Acids

35.83

Weight (kDa)

7.14

Isoelectric Point (pI)

61.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 160 - 246 5.1e-09 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017669)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19881 FvH4_6g29430
prunus_persica Prupe.5G061400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204681
rosa_laevigata RLG00000003438
rosa_multiflora Rmu_sc0007104.1_g000008
rosa_rugosa Rorug07G0085600
rosa_samantha Rh7AG215500 Rh7BG212700 Rh7CG228600 Rh7DG223200
rosa_wichuraiana Rw7G018680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 66
Acc65I GGTACC 1 cut(s) 741
AccB1I GGYRCC 1 cut(s) 741
AccBSI CCGCTC 1 cut(s) 615
AccII CGCG 1 cut(s) 268
AciI CCGC 2 cut(s) 266, 613
AclWI GGATC 1 cut(s) 182
AcsI RAATTY 2 cut(s) 394, 767
AcuI CTGAAG 2 cut(s) 374, 725
AfaI GTAC 6 cut(s) 94, 335, 377, 649, 743, 796
AfeI AGCGCT 1 cut(s) 319
AfiI CCNNNNNNNGG 1 cut(s) 152
AgsI TTSAA 4 cut(s) 406, 414, 530, 859
AhlI ACTAGT 2 cut(s) 668, 884
AjnI CCWGG 1 cut(s) 522
AjuI GAANNNNNNNTTGG 2 cut(s) 851, 883
AluBI AGCT 7 cut(s) 233, 251, 272, 585, 657, 855, 913
AluI AGCT 7 cut(s) 233, 251, 272, 585, 657, 855, 913
Alw21I GWGCWC 1 cut(s) 274
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 1 cut(s) 182
Ama87I CYCGRG 1 cut(s) 689
Aor51HI AGCGCT 1 cut(s) 319
ApeKI GCWGC 2 cut(s) 230, 251
ApoI RAATTY 2 cut(s) 394, 767
AseI ATTAAT 1 cut(s) 780
Asp718I GGTACC 1 cut(s) 741
AspLEI GCGC 1 cut(s) 320
AsuHPI GGTGA 2 cut(s) 169, 904
AvaI CYCGRG 1 cut(s) 689
BanI GGYRCC 1 cut(s) 741
BanII GRGCYC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 274
BbvI GCAGC 2 cut(s) 238, 242
BccI CCATC 2 cut(s) 31, 82
BciT130I CCWGG 1 cut(s) 524
BcoDI GTCTC 1 cut(s) 675
BcuI ACTAGT 2 cut(s) 668, 884
BfaI CTAG 2 cut(s) 669, 885
BfmI CTRYAG 3 cut(s) 252, 798, 825
BfoI RGCGCY 1 cut(s) 321
BfuAI ACCTGC 1 cut(s) 66
BisI GCNGC 4 cut(s) 231, 252, 266, 613
BlsI GCNGC 4 cut(s) 232, 253, 267, 614
BmcAI AGTACT 2 cut(s) 335, 796
Bme1390I CCNGG 1 cut(s) 524
BmeT110I CYCGRG 1 cut(s) 689
BmiI GGNNCC 1 cut(s) 743
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 5 cut(s) 252, 375, 551, 725, 742
BplI GAGNNNNNCTC 2 cut(s) 672, 704
Bsa29I ATCGAT 1 cut(s) 383
BsaBI GATNNNNATC 1 cut(s) 606
BsaJI CCNNGG 1 cut(s) 745
BsaWI WCCGGW 1 cut(s) 291
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse118I RCCGGY 1 cut(s) 738
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 1 cut(s) 524
BseCI ATCGAT 1 cut(s) 383
BseDI CCNNGG 1 cut(s) 745
BseGI GGATG 5 cut(s) 16, 363, 508, 635, 757
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 1 cut(s) 152
BseMII CTCAG 3 cut(s) 344, 482, 779
BseRI GAGGAG 1 cut(s) 112
BseXI GCAGC 2 cut(s) 238, 242
BseYI CCCAGC 1 cut(s) 675
Bsh1236I CGCG 1 cut(s) 268
BshNI GGYRCC 1 cut(s) 741
BshVI ATCGAT 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 274
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 2 cut(s) 292, 739
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 152
BsmAI GTCTC 1 cut(s) 675
BsmFI GGGAC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 689
Bsp1286I GDGCHC 1 cut(s) 274
Bsp143I GATC 2 cut(s) 49, 174
BspACI CCGC 2 cut(s) 266, 613
BspCNI CTCAG 3 cut(s) 345, 483, 780
BspDI ATCGAT 1 cut(s) 383
BspFNI CGCG 1 cut(s) 268
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 743
BspMAI CTGCAG 2 cut(s) 256, 802
BspMI ACCTGC 1 cut(s) 66
BspPI GGATC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 741
BsrBI CCGCTC 1 cut(s) 615
BsrFI RCCGGY 1 cut(s) 738
BssAI RCCGGY 1 cut(s) 738
BssECI CCNNGG 1 cut(s) 745
BssMI GATC 2 cut(s) 49, 174
BssT1I CCWWGG 1 cut(s) 745
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 3 cut(s) 353, 491, 788
BstF5I GGATG 5 cut(s) 16, 363, 508, 635, 757
BstFNI CGCG 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 2 cut(s) 52, 177
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 2 cut(s) 49, 174
BstMWI GCNNNNNNNGC 3 cut(s) 199, 236, 582
BstNI CCWGG 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 522
BstSFI CTRYAG 3 cut(s) 252, 798, 825
BstUI CGCG 1 cut(s) 268
BstV1I GCAGC 2 cut(s) 238, 242
Bsu15I ATCGAT 1 cut(s) 383
BsuTUI ATCGAT 1 cut(s) 383
BtgZI GCGATG 1 cut(s) 593
BtsCI GGATG 5 cut(s) 16, 363, 508, 635, 757
BtsIMutI CAGTG 2 cut(s) 146, 310
BveI ACCTGC 1 cut(s) 66
Cac8I GCNNGC 1 cut(s) 270
CciI TCATGA 1 cut(s) 444
CfoI GCGC 1 cut(s) 320
Cfr10I RCCGGY 1 cut(s) 738
ClaI ATCGAT 1 cut(s) 383
Csp6I GTAC 6 cut(s) 93, 334, 376, 648, 742, 795
CviAII CATG 3 cut(s) 431, 445, 618
CviQI GTAC 6 cut(s) 93, 334, 376, 648, 742, 795
DdeI CTNAG 3 cut(s) 353, 491, 788
DpnI GATC 2 cut(s) 51, 176
DpnII GATC 2 cut(s) 49, 174
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Ecl136II GAGCTC 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 745
Eco24I GRGCYC 1 cut(s) 274
Eco47III AGCGCT 1 cut(s) 319
Eco53kI GAGCTC 1 cut(s) 272
Eco57I CTGAAG 2 cut(s) 374, 725
Eco88I CYCGRG 1 cut(s) 689
EcoICRI GAGCTC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 522
EcoT14I CCWWGG 1 cut(s) 745
EcoT22I ATGCAT 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 745
FaeI CATG 3 cut(s) 434, 448, 621
FaqI GGGAC 1 cut(s) 220
FatI CATG 3 cut(s) 430, 444, 617
FauNDI CATATG 4 cut(s) 211, 241, 466, 498
Fnu4HI GCNGC 4 cut(s) 231, 252, 266, 613
FokI GGATG 5 cut(s) 23, 370, 515, 642, 764
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 4 cut(s) 231, 252, 266, 613
FspBI CTAG 2 cut(s) 669, 885
GlaI GCGC 1 cut(s) 319
GluI GCNGC 4 cut(s) 231, 252, 266, 613
GsaI CCCAGC 1 cut(s) 679
HaeII RGCGCY 1 cut(s) 321
HapII CCGG 2 cut(s) 292, 739
HhaI GCGC 1 cut(s) 320
Hin1II CATG 3 cut(s) 434, 448, 621
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HincII GTYRAC 1 cut(s) 107
HindII GTYRAC 1 cut(s) 107
HindIII AAGCTT 2 cut(s) 655, 853
HinfI GANTC 3 cut(s) 23, 406, 493
HpaII CCGG 2 cut(s) 292, 739
HphI GGTGA 2 cut(s) 169, 904
Hpy166II GTNNAC 2 cut(s) 107, 650
Hpy188I TCNGA 5 cut(s) 22, 66, 84, 354, 393
Hpy188III TCNNGA 5 cut(s) 259, 445, 689, 691, 760
Hpy8I GTNNAC 2 cut(s) 107, 650
HpyAV CCTTC 5 cut(s) 442, 451, 478, 566, 700
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 7 cut(s) 46, 254, 364, 434, 542, 800, 882
HpyF10VI GCNNNNNNNGC 3 cut(s) 199, 236, 582
HpyF3I CTNAG 3 cut(s) 353, 491, 788
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 3 cut(s) 434, 448, 621
HspAI GCGC 1 cut(s) 318
KpnI GGTACC 1 cut(s) 745
Kzo9I GATC 2 cut(s) 49, 174
Lsp1109I GCAGC 2 cut(s) 238, 242
LweI GCATC 5 cut(s) 252, 375, 551, 725, 742
MaeI CTAG 2 cut(s) 669, 885
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 505
MalI GATC 2 cut(s) 51, 176
MbiI CCGCTC 1 cut(s) 615
MboI GATC 2 cut(s) 49, 174
MboII GAAGA 3 cut(s) 108, 323, 578
MfeI CAATTG 1 cut(s) 339
MhlI GDGCHC 1 cut(s) 274
MluCI AATT 5 cut(s) 298, 339, 394, 726, 767
MlyI GAGTC 2 cut(s) 17, 502
MmeI TCCRAC 3 cut(s) 89, 403, 849
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 4 cut(s) 480, 780, 909, 937
MslI CAYNNNNRTG 2 cut(s) 501, 628
MspI CCGG 2 cut(s) 292, 739
MspR9I CCNGG 1 cut(s) 524
MunI CAATTG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 524
MvnI CGCG 1 cut(s) 268
MwoI GCNNNNNNNGC 3 cut(s) 199, 236, 582
NdeI CATATG 4 cut(s) 211, 241, 466, 498
NdeII GATC 2 cut(s) 49, 174
NlaIII CATG 3 cut(s) 434, 448, 621
NlaIV GGNNCC 1 cut(s) 743
NmuCI GTSAC 1 cut(s) 505
NsiI ATGCAT 1 cut(s) 544
PaeR7I CTCGAG 1 cut(s) 689
PagI TCATGA 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 380
PfeI GAWTC 1 cut(s) 406
PkrI GCNGC 4 cut(s) 232, 253, 267, 614
PleI GAGTC 2 cut(s) 17, 501
PpsI GAGTC 2 cut(s) 17, 501
PshBI ATTAAT 1 cut(s) 780
Psp124BI GAGCTC 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 522
PspFI CCCAGC 1 cut(s) 675
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 1 cut(s) 743
PstI CTGCAG 2 cut(s) 256, 802
RsaI GTAC 6 cut(s) 94, 335, 377, 649, 743, 796
RsaNI GTAC 6 cut(s) 93, 334, 376, 648, 742, 795
RseI CAYNNNNRTG 2 cut(s) 501, 628
SacI GAGCTC 1 cut(s) 274
SaqAI TTAA 4 cut(s) 480, 780, 909, 937
SatI GCNGC 4 cut(s) 231, 252, 266, 613
Sau3AI GATC 2 cut(s) 49, 174
ScaI AGTACT 2 cut(s) 335, 796
SchI GAGTC 2 cut(s) 17, 502
ScrFI CCNGG 1 cut(s) 524
SduI GDGCHC 1 cut(s) 274
SfaNI GCATC 5 cut(s) 252, 375, 551, 725, 742
SfcI CTRYAG 3 cut(s) 252, 798, 825
Sfr274I CTCGAG 1 cut(s) 689
SlaI CTCGAG 1 cut(s) 689
SmiMI CAYNNNNRTG 2 cut(s) 501, 628
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
SpeI ACTAGT 2 cut(s) 668, 884
Sse9I AATT 5 cut(s) 298, 339, 394, 726, 767
SsiI CCGC 2 cut(s) 266, 613
SspMI CTAG 2 cut(s) 669, 885
SstI GAGCTC 1 cut(s) 274
StyD4I CCNGG 1 cut(s) 522
StyI CCWWGG 1 cut(s) 745
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 377
TaqI TCGA 2 cut(s) 383, 690
TasI AATT 5 cut(s) 298, 339, 394, 726, 767
TatI WGTACW 3 cut(s) 333, 647, 794
TauI GCSGC 2 cut(s) 268, 615
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 4 cut(s) 480, 780, 909, 937
Tru9I TTAA 4 cut(s) 480, 780, 909, 937
TscAI CASTG 2 cut(s) 153, 310
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 2 cut(s) 230, 251
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 7 cut(s) 228, 419, 433, 453, 461, 585, 624
TspRI CASTG 2 cut(s) 153, 310
VspI ATTAAT 1 cut(s) 780
XapI RAATTY 2 cut(s) 394, 767
XhoI CTCGAG 1 cut(s) 689
XspI CTAG 2 cut(s) 669, 885
ZrmI AGTACT 2 cut(s) 335, 796
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.