Rh7BG212700

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0017669
Physical Location & Seq
Reverse (-)
18081008 .. 18081940
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG212700.1

Sequence Viewer

Length: 933 bp
ATGGATGTCAGTTCTGACTCAACAATAGAGATGGTGGTGCAAGATCAAAACTTGCCTCCGACCTCTCCATCTCATCAGAATAGATGTACGAGAAGCAGTCAACAAGGTTCTTCCTCCTCTAAACTCAAAGGAGTGATACCACTGAAAGGTGGTATGTGGGGGGCAAGGATCACCTATAACTACAAGCGATACAGGCTTGGGACATATGAAACAGAGATCGAGGCAGCTAGAGCATATGATAGAGCTGCAGTCAAGATGCCGCGAGCTCATTCCCTAAACTTCCACCGGAGTAATTACAGTGTAGAAGAGAGCGCTTTCCAAAGTCAGTGCTCAATTGAGGAAGTTCTGAGGATGTTGCAGGATAAAACGTACTCATCGATGCTTCAGAATTTTATTTTGAATCATTCAACTTGTGTTGGATTTCATGCAGAAGCCTTCATGAAGGAACAAGGGATTTCATATGACTTTCTCTTTAAGAAGGAACTGAGTCATATGGATGTCACTCACAAGTGCTTCCTGGTTCAAGGGGATTATGCATCACTACATTCCCCCCCAATAGAAGATGAAGGCTGGAATAGCTTTGCCACTTTGTATGATATACATCGCCGCTCATGGACATTTGGATGTTCATATTGGCAGAGTACACAAAGCTTTATTTTTACTAGTGGCTGGGGAGACTTTCTCGAGATGCATAACCTGAAGGAGAAAGACAAAATAATAATTTACAGATGCCGGTACCAAGGGGATGCTGTCGGGAGAAAATTTCACTTGATTAATGTTCTGAGAAGTACTGCAGAAAGTTCTGTTGTTGGTATGACTACAGAGCAAGATGTCGGTTTGAGAGGAAGCTTGAACAAGGAGTTGGAGGCTTATTGCACTAGTGAGGTGAAAAAGAGAGGGTTTAAGCTCTTTGGTATTGAGATTAGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

310

Amino Acids

35.43

Weight (kDa)

7.64

Isoelectric Point (pI)

58.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 158 - 244 5.3e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017669)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19881 FvH4_6g29430
prunus_persica Prupe.5G061400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204681
rosa_laevigata RLG00000003438
rosa_multiflora Rmu_sc0007104.1_g000008
rosa_rugosa Rorug07G0085600
rosa_samantha Rh7AG215500 Rh7BG212700 Rh7CG228600 Rh7DG223200
rosa_wichuraiana Rw7G018680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 735
AccB1I GGYRCC 1 cut(s) 735
AccBSI CCGCTC 1 cut(s) 609
AccII CGCG 1 cut(s) 262
AciI CCGC 2 cut(s) 260, 607
AclWI GGATC 1 cut(s) 176
AcsI RAATTY 2 cut(s) 388, 761
AcuI CTGAAG 2 cut(s) 368, 719
AfaI GTAC 5 cut(s) 88, 371, 643, 737, 790
AfeI AGCGCT 1 cut(s) 313
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 4 cut(s) 400, 408, 524, 853
AhlI ACTAGT 2 cut(s) 662, 878
AjnI CCWGG 1 cut(s) 516
AjuI GAANNNNNNNTTGG 2 cut(s) 845, 877
AluBI AGCT 7 cut(s) 227, 245, 266, 579, 651, 849, 907
AluI AGCT 7 cut(s) 227, 245, 266, 579, 651, 849, 907
Alw21I GWGCWC 2 cut(s) 268, 332
Alw26I GTCTC 1 cut(s) 669
AlwI GGATC 1 cut(s) 176
Ama87I CYCGRG 1 cut(s) 683
Aor51HI AGCGCT 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 224, 245
ApoI RAATTY 2 cut(s) 388, 761
AseI ATTAAT 1 cut(s) 774
Asp718I GGTACC 1 cut(s) 735
AspLEI GCGC 1 cut(s) 314
AsuHPI GGTGA 2 cut(s) 163, 898
AvaI CYCGRG 1 cut(s) 683
BanI GGYRCC 1 cut(s) 735
BanII GRGCYC 1 cut(s) 268
Bbv12I GWGCWC 2 cut(s) 268, 332
BbvI GCAGC 2 cut(s) 232, 236
BccI CCATC 2 cut(s) 25, 76
BciT130I CCWGG 1 cut(s) 518
BcoDI GTCTC 1 cut(s) 669
BcuI ACTAGT 2 cut(s) 662, 878
BfaI CTAG 3 cut(s) 228, 663, 879
BfmI CTRYAG 3 cut(s) 246, 792, 819
BfoI RGCGCY 1 cut(s) 315
BisI GCNGC 4 cut(s) 225, 246, 260, 607
BlsI GCNGC 4 cut(s) 226, 247, 261, 608
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 1 cut(s) 518
BmeT110I CYCGRG 1 cut(s) 683
BmiI GGNNCC 1 cut(s) 737
BmrFI CCNGG 1 cut(s) 518
BmsI GCATC 6 cut(s) 246, 369, 545, 678, 719, 736
BplI GAGNNNNNCTC 2 cut(s) 666, 698
Bsa29I ATCGAT 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 1 cut(s) 739
BsaWI WCCGGW 1 cut(s) 285
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 600
BseBI CCWGG 1 cut(s) 518
BseCI ATCGAT 1 cut(s) 377
BseDI CCNNGG 1 cut(s) 739
BseGI GGATG 5 cut(s) 10, 357, 502, 629, 751
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMII CTCAG 3 cut(s) 338, 476, 773
BseRI GAGGAG 1 cut(s) 106
BseXI GCAGC 2 cut(s) 232, 236
BseYI CCCAGC 1 cut(s) 669
Bsh1236I CGCG 1 cut(s) 262
BshNI GGYRCC 1 cut(s) 735
BshVI ATCGAT 1 cut(s) 377
BsiHKAI GWGCWC 2 cut(s) 268, 332
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 2 cut(s) 286, 733
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 669
BsmFI GGGAC 1 cut(s) 214
BsoBI CYCGRG 1 cut(s) 683
Bsp1286I GDGCHC 2 cut(s) 268, 332
Bsp143I GATC 3 cut(s) 43, 168, 216
BspACI CCGC 2 cut(s) 260, 607
BspCNI CTCAG 3 cut(s) 339, 477, 774
BspDI ATCGAT 1 cut(s) 377
BspFNI CGCG 1 cut(s) 262
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 737
BspMAI CTGCAG 2 cut(s) 250, 796
BspPI GGATC 1 cut(s) 176
BspT107I GGYRCC 1 cut(s) 735
BsrBI CCGCTC 1 cut(s) 609
BsrFI RCCGGY 1 cut(s) 732
BssAI RCCGGY 1 cut(s) 732
BssECI CCNNGG 1 cut(s) 739
BssMI GATC 3 cut(s) 43, 168, 216
BssT1I CCWWGG 1 cut(s) 739
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 3 cut(s) 347, 485, 782
BstF5I GGATG 5 cut(s) 10, 357, 502, 629, 751
BstFNI CGCG 1 cut(s) 262
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 3 cut(s) 46, 171, 219
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 3 cut(s) 43, 168, 216
BstMWI GCNNNNNNNGC 3 cut(s) 193, 230, 576
BstNI CCWGG 1 cut(s) 518
BstSCI CCNGG 1 cut(s) 516
BstSFI CTRYAG 3 cut(s) 246, 792, 819
BstUI CGCG 1 cut(s) 262
BstV1I GCAGC 2 cut(s) 232, 236
Bsu15I ATCGAT 1 cut(s) 377
BsuTUI ATCGAT 1 cut(s) 377
BtgZI GCGATG 1 cut(s) 587
BtsCI GGATG 5 cut(s) 10, 357, 502, 629, 751
BtsIMutI CAGTG 3 cut(s) 140, 304, 332
Cac8I GCNNGC 1 cut(s) 264
CciI TCATGA 1 cut(s) 438
CfoI GCGC 1 cut(s) 314
Cfr10I RCCGGY 1 cut(s) 732
ClaI ATCGAT 1 cut(s) 377
Csp6I GTAC 5 cut(s) 87, 370, 642, 736, 789
CviAII CATG 3 cut(s) 425, 439, 612
CviQI GTAC 5 cut(s) 87, 370, 642, 736, 789
DdeI CTNAG 3 cut(s) 347, 485, 782
DpnI GATC 3 cut(s) 45, 170, 218
DpnII GATC 3 cut(s) 43, 168, 216
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 266
Eco130I CCWWGG 1 cut(s) 739
Eco24I GRGCYC 1 cut(s) 268
Eco47III AGCGCT 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 2 cut(s) 368, 719
Eco88I CYCGRG 1 cut(s) 683
EcoICRI GAGCTC 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 516
EcoT14I CCWWGG 1 cut(s) 739
EcoT22I ATGCAT 2 cut(s) 538, 693
EcoT38I GRGCYC 1 cut(s) 268
ErhI CCWWGG 1 cut(s) 739
FaeI CATG 3 cut(s) 428, 442, 615
FaqI GGGAC 1 cut(s) 214
FatI CATG 3 cut(s) 424, 438, 611
FauNDI CATATG 4 cut(s) 205, 235, 460, 492
Fnu4HI GCNGC 4 cut(s) 225, 246, 260, 607
FokI GGATG 5 cut(s) 17, 364, 509, 636, 758
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 4 cut(s) 225, 246, 260, 607
FspBI CTAG 3 cut(s) 228, 663, 879
GlaI GCGC 1 cut(s) 313
GluI GCNGC 4 cut(s) 225, 246, 260, 607
GsaI CCCAGC 1 cut(s) 673
HaeII RGCGCY 1 cut(s) 315
HapII CCGG 2 cut(s) 286, 733
HhaI GCGC 1 cut(s) 314
Hin1II CATG 3 cut(s) 428, 442, 615
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HincII GTYRAC 1 cut(s) 101
HindII GTYRAC 1 cut(s) 101
HindIII AAGCTT 2 cut(s) 649, 847
HinfI GANTC 3 cut(s) 17, 400, 487
HpaII CCGG 2 cut(s) 286, 733
HphI GGTGA 2 cut(s) 163, 898
Hpy166II GTNNAC 2 cut(s) 101, 644
Hpy188I TCNGA 6 cut(s) 16, 60, 78, 348, 387, 783
Hpy188III TCNNGA 5 cut(s) 253, 439, 683, 685, 754
Hpy8I GTNNAC 2 cut(s) 101, 644
HpyAV CCTTC 5 cut(s) 436, 445, 472, 560, 694
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 8 cut(s) 40, 248, 358, 428, 536, 691, 794, 876
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 230, 576
HpyF3I CTNAG 3 cut(s) 347, 485, 782
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 3 cut(s) 428, 442, 615
HspAI GCGC 1 cut(s) 312
KpnI GGTACC 1 cut(s) 739
Kzo9I GATC 3 cut(s) 43, 168, 216
LpnPI CCDG 9 cut(s) 178, 299, 344, 503, 530, 556, 655, 710, 746
Lsp1109I GCAGC 2 cut(s) 232, 236
LweI GCATC 6 cut(s) 246, 369, 545, 678, 719, 736
MaeI CTAG 3 cut(s) 228, 663, 879
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 1 cut(s) 499
MalI GATC 3 cut(s) 45, 170, 218
MbiI CCGCTC 1 cut(s) 609
MboI GATC 3 cut(s) 43, 168, 216
MboII GAAGA 3 cut(s) 102, 317, 572
MfeI CAATTG 1 cut(s) 333
MhlI GDGCHC 2 cut(s) 268, 332
MluCI AATT 5 cut(s) 292, 333, 388, 720, 761
MlyI GAGTC 2 cut(s) 11, 496
MmeI TCCRAC 3 cut(s) 83, 397, 843
Mph1103I ATGCAT 2 cut(s) 538, 693
MseI TTAA 4 cut(s) 474, 774, 903, 931
MslI CAYNNNNRTG 2 cut(s) 495, 622
MspI CCGG 2 cut(s) 286, 733
MspR9I CCNGG 1 cut(s) 518
MunI CAATTG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 518
MvnI CGCG 1 cut(s) 262
MwoI GCNNNNNNNGC 3 cut(s) 193, 230, 576
NdeI CATATG 4 cut(s) 205, 235, 460, 492
NdeII GATC 3 cut(s) 43, 168, 216
NlaIII CATG 3 cut(s) 428, 442, 615
NlaIV GGNNCC 1 cut(s) 737
NmuCI GTSAC 1 cut(s) 499
NsiI ATGCAT 2 cut(s) 538, 693
PaeR7I CTCGAG 1 cut(s) 683
PagI TCATGA 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 374
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 4 cut(s) 226, 247, 261, 608
PleI GAGTC 2 cut(s) 11, 495
PpsI GAGTC 2 cut(s) 11, 495
PshBI ATTAAT 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 516
PspFI CCCAGC 1 cut(s) 669
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 1 cut(s) 737
PstI CTGCAG 2 cut(s) 250, 796
RsaI GTAC 5 cut(s) 88, 371, 643, 737, 790
RsaNI GTAC 5 cut(s) 87, 370, 642, 736, 789
RseI CAYNNNNRTG 2 cut(s) 495, 622
SacI GAGCTC 1 cut(s) 268
SaqAI TTAA 4 cut(s) 474, 774, 903, 931
SatI GCNGC 4 cut(s) 225, 246, 260, 607
Sau3AI GATC 3 cut(s) 43, 168, 216
ScaI AGTACT 1 cut(s) 790
SchI GAGTC 2 cut(s) 11, 496
ScrFI CCNGG 1 cut(s) 518
SduI GDGCHC 2 cut(s) 268, 332
SfaNI GCATC 6 cut(s) 246, 369, 545, 678, 719, 736
SfcI CTRYAG 3 cut(s) 246, 792, 819
Sfr274I CTCGAG 1 cut(s) 683
SlaI CTCGAG 1 cut(s) 683
SmiMI CAYNNNNRTG 2 cut(s) 495, 622
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
SpeI ACTAGT 2 cut(s) 662, 878
Sse9I AATT 5 cut(s) 292, 333, 388, 720, 761
SsiI CCGC 2 cut(s) 260, 607
SspMI CTAG 3 cut(s) 228, 663, 879
SstI GAGCTC 1 cut(s) 268
StyD4I CCNGG 1 cut(s) 516
StyI CCWWGG 1 cut(s) 739
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 1 cut(s) 371
TaqI TCGA 3 cut(s) 219, 377, 684
TasI AATT 5 cut(s) 292, 333, 388, 720, 761
TatI WGTACW 2 cut(s) 641, 788
TauI GCSGC 2 cut(s) 262, 609
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 4 cut(s) 474, 774, 903, 931
Tru9I TTAA 4 cut(s) 474, 774, 903, 931
TscAI CASTG 3 cut(s) 147, 304, 332
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 2 cut(s) 224, 245
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 7 cut(s) 222, 413, 427, 447, 455, 579, 618
TspRI CASTG 3 cut(s) 147, 304, 332
VspI ATTAAT 1 cut(s) 774
XapI RAATTY 2 cut(s) 388, 761
XhoI CTCGAG 1 cut(s) 683
XspI CTAG 3 cut(s) 228, 663, 879
ZrmI AGTACT 1 cut(s) 790
Zsp2I ATGCAT 2 cut(s) 538, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.