Prupe.5G061400_v2.0.a1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0017669
Physical Location & Seq
Forward (+)
6722569 .. 6723411
843 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G061400.1

Sequence Viewer

Length: 843 bp
ATGGTGTTACAAAGAGGCCCTTCCTCCAGATTCAAAGGGGTAAGTCTGCTGAAAAGTGGTAACTGGGGTGCTAGGATCTCCTATATGTACAAGCCATACTGGCTTGGAGCATATCAGATGGAGGAAGAGGCAGCTATTGCTTATGACAGAGCAGCCGTCAAGCTTCAAAGAGGTGATACCCAACTCAACTTTCATTGGAGTAAATACAGTGCTGAAGAGAGCATATTCCAAAGTCAGTACTCAACTGAGGAAATTCTGAGTATGTTAAAGGATAAAACTTACTCATCCAAACTTATGAATTTTCTTTCAAGGCATTCATCAGGTTTGGGAACTCGAACAACAACCTTCATGAATGAACAAGGGATTTCATATCACTTTCTCTTTCAGAAGGAACTGACTCATAGAGATGTCACTAACAAGTGCTTTCTGATCCCAAGGGAATTTTCATTTCAATACCTTCCCCCACCTGAAGGCATCATCAATGCAGGAGAAGATGAAGCTAGGTACGTTAAATTAACCTTTTATGATAAACATGGCCGTTCTTGGACATTTGGATATTCATATTGGCAGAGTACGCGGAACTTTATGTTTACAAATGGCTGGGGACACTTTATCAGGATGAATAACCTGAACCAGAAAGACTTAGTAATGTTTTACAAATGCGAGTATGAAGGGGAGGTTCTCGGGAGAGAAGTTTACATGATAGACTATCAAAGAAGCAATGGGGAAAACTATGTCCTAGAGCATTCTTCAGGAAGCAGTACCATAGAGTTAGAGGCTGATTGCAAGCAAAAAGAGACTAAGAAAGGATTCAAGCTATTTGGTGTGGAGATTAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

281

Amino Acids

32.75

Weight (kDa)

8.24

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017669)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19881 FvH4_6g29430
prunus_persica Prupe.5G061400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204681
rosa_laevigata RLG00000003438
rosa_multiflora Rmu_sc0007104.1_g000008
rosa_rugosa Rorug07G0085600
rosa_samantha Rh7AG215500 Rh7BG212700 Rh7CG228600 Rh7DG223200
rosa_wichuraiana Rw7G018680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 577
AciI CCGC 1 cut(s) 577
AclWI GGATC 2 cut(s) 83, 424
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 3 cut(s) 252, 298, 440
AcuI CTGAAG 3 cut(s) 234, 489, 735
AfaI GTAC 5 cut(s) 89, 239, 506, 574, 763
AfiI CCNNNNNNNGG 1 cut(s) 470
AgsI TTSAA 5 cut(s) 34, 167, 309, 452, 814
AjuI GAANNNNNNNTTGG 4 cut(s) 174, 206, 427, 459
AluBI AGCT 4 cut(s) 134, 163, 500, 817
AluI AGCT 4 cut(s) 134, 163, 500, 817
Alw26I GTCTC 1 cut(s) 791
AlwI GGATC 2 cut(s) 83, 424
Ama87I CYCGRG 1 cut(s) 683
AoxI GGCC 2 cut(s) 16, 535
ApeKI GCWGC 2 cut(s) 131, 152
ApoI RAATTY 3 cut(s) 252, 298, 440
AseI ATTAAT 1 cut(s) 834
Asp700I GAANNNNTTC 1 cut(s) 809
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 683
BbvI GCAGC 2 cut(s) 143, 164
BccI CCATC 1 cut(s) 112
BceAI ACGGC 2 cut(s) 140, 522
BcoDI GTCTC 1 cut(s) 791
BfaI CTAG 3 cut(s) 72, 501, 740
BglI GCCNNNNNGGC 1 cut(s) 100
BisI GCNGC 2 cut(s) 132, 153
BlsI GCNGC 2 cut(s) 133, 154
BmcAI AGTACT 1 cut(s) 239
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 17
BmrI ACTGGG 1 cut(s) 73
BmsI GCATC 1 cut(s) 483
BmuI ACTGGG 1 cut(s) 73
BpmI CTGGAG 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 434
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 68, 104
Bse3DI GCAATG 1 cut(s) 727
BseDI CCNNGG 1 cut(s) 434
BseGI GGATG 2 cut(s) 284, 624
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 2 cut(s) 237, 248
BseNI ACTGG 2 cut(s) 68, 104
BseXI GCAGC 2 cut(s) 143, 164
BseYI CCCAGC 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 577
BshFI GGCC 2 cut(s) 18, 537
BsiHKCI CYCGRG 1 cut(s) 683
BslFI GGGAC 1 cut(s) 618
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 1 cut(s) 791
BsmFI GGGAC 1 cut(s) 618
BsmI GAATGC 2 cut(s) 313, 745
BsnI GGCC 2 cut(s) 18, 537
BsoBI CYCGRG 1 cut(s) 683
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 2 cut(s) 75, 429
BspACI CCGC 1 cut(s) 577
BspANI GGCC 2 cut(s) 18, 537
BspCNI CTCAG 2 cut(s) 238, 249
BspFNI CGCG 1 cut(s) 577
BspHI TCATGA 1 cut(s) 348
BspPI GGATC 2 cut(s) 83, 424
BsrDI GCAATG 1 cut(s) 727
BsrGI TGTACA 1 cut(s) 87
BsrI ACTGG 2 cut(s) 68, 104
BssECI CCNNGG 1 cut(s) 434
BssMI GATC 2 cut(s) 75, 429
BssT1I CCWWGG 1 cut(s) 434
Bst4CI ACNGT 1 cut(s) 209
Bst6I CTCTTC 2 cut(s) 120, 210
BstAPI GCANNNNNTGC 1 cut(s) 137
BstAUI TGTACA 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 788
BstDEI CTNAG 4 cut(s) 246, 257, 643, 801
BstF5I GGATG 2 cut(s) 284, 624
BstFNI CGCG 1 cut(s) 577
BstKTI GATC 2 cut(s) 78, 432
BstMAI GTCTC 1 cut(s) 791
BstMBI GATC 2 cut(s) 75, 429
BstMWI GCNNNNNNNGC 3 cut(s) 100, 137, 574
BstUI CGCG 1 cut(s) 577
BstV1I GCAGC 2 cut(s) 143, 164
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BsuRI GGCC 2 cut(s) 18, 537
BtsCI GGATG 2 cut(s) 284, 624
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 1 cut(s) 788
CciI TCATGA 1 cut(s) 348
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 5 cut(s) 88, 238, 505, 573, 762
CviAII CATG 3 cut(s) 349, 533, 700
CviQI GTAC 5 cut(s) 88, 238, 505, 573, 762
DdeI CTNAG 4 cut(s) 246, 257, 643, 801
DpnI GATC 2 cut(s) 77, 431
DpnII GATC 2 cut(s) 75, 429
EaeI YGGCCR 1 cut(s) 535
Eam1104I CTCTTC 2 cut(s) 120, 210
EarI CTCTTC 2 cut(s) 120, 210
Eco130I CCWWGG 1 cut(s) 434
Eco57I CTGAAG 3 cut(s) 234, 489, 735
Eco88I CYCGRG 1 cut(s) 683
EcoO109I RGGNCCY 1 cut(s) 17
EcoT14I CCWWGG 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 434
FaeI CATG 3 cut(s) 352, 536, 703
FalI AAGNNNNNCTT 1 cut(s) 36
FaqI GGGAC 1 cut(s) 618
FatI CATG 3 cut(s) 348, 532, 699
Fnu4HI GCNGC 2 cut(s) 132, 153
FokI GGATG 2 cut(s) 271, 631
Fsp4HI GCNGC 2 cut(s) 132, 153
FspBI CTAG 3 cut(s) 72, 501, 740
GluI GCNGC 2 cut(s) 132, 153
GsaI CCCAGC 1 cut(s) 604
GsuI CTGGAG 1 cut(s) 10
HaeIII GGCC 2 cut(s) 18, 537
Hin1II CATG 3 cut(s) 352, 536, 703
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 3 cut(s) 30, 397, 810
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 2 cut(s) 591, 697
Hpy188I TCNGA 4 cut(s) 117, 258, 387, 429
Hpy188III TCNNGA 5 cut(s) 27, 349, 616, 685, 753
Hpy8I GTNNAC 2 cut(s) 591, 697
HpyAV CCTTC 6 cut(s) 30, 355, 382, 464, 467, 665
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 2 cut(s) 485, 786
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 137, 574
HpyF3I CTNAG 4 cut(s) 246, 257, 643, 801
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 3 cut(s) 352, 536, 703
Kzo9I GATC 2 cut(s) 75, 429
LmnI GCTCC 1 cut(s) 107
Lsp1109I GCAGC 2 cut(s) 143, 164
LweI GCATC 1 cut(s) 483
MaeI CTAG 3 cut(s) 72, 501, 740
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 3 cut(s) 6, 59, 409
MalI GATC 2 cut(s) 77, 431
MboI GATC 2 cut(s) 75, 429
MboII GAAGA 4 cut(s) 137, 227, 503, 741
MflI RGATCY 1 cut(s) 75
MluCI AATT 4 cut(s) 252, 298, 440, 512
MlyI GAGTC 1 cut(s) 391
MnlI CCTC 8 cut(s) 8, 34, 115, 121, 164, 241, 670, 769
MroXI GAANNNNTTC 1 cut(s) 809
MseI TTAA 5 cut(s) 266, 510, 515, 834, 841
MslI CAYNNNNRTG 1 cut(s) 405
Mva1269I GAATGC 2 cut(s) 313, 745
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 3 cut(s) 100, 137, 574
NdeII GATC 2 cut(s) 75, 429
NlaIII CATG 3 cut(s) 352, 536, 703
NmuCI GTSAC 1 cut(s) 409
PagI TCATGA 1 cut(s) 348
PctI GAATGC 2 cut(s) 313, 745
PdmI GAANNNNTTC 1 cut(s) 809
PfeI GAWTC 2 cut(s) 30, 810
PkrI GCNGC 2 cut(s) 133, 154
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
PshBI ATTAAT 1 cut(s) 834
PspFI CCCAGC 1 cut(s) 600
PspPI GGNCC 1 cut(s) 17
PsuI RGATCY 1 cut(s) 75
RsaI GTAC 5 cut(s) 89, 239, 506, 574, 763
RsaNI GTAC 5 cut(s) 88, 238, 505, 573, 762
RseI CAYNNNNRTG 1 cut(s) 405
SaqAI TTAA 5 cut(s) 266, 510, 515, 834, 841
SatI GCNGC 2 cut(s) 132, 153
Sau3AI GATC 2 cut(s) 75, 429
Sau96I GGNCC 1 cut(s) 17
ScaI AGTACT 1 cut(s) 239
SchI GAGTC 1 cut(s) 391
SfaNI GCATC 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 405
Sse9I AATT 4 cut(s) 252, 298, 440, 512
SsiI CCGC 1 cut(s) 577
SspMI CTAG 3 cut(s) 72, 501, 740
StyI CCWWGG 1 cut(s) 434
TaaI ACNGT 1 cut(s) 209
TaiI ACGT 1 cut(s) 510
TaqI TCGA 1 cut(s) 334
TasI AATT 4 cut(s) 252, 298, 440, 512
TatI WGTACW 2 cut(s) 87, 237
TfiI GAWTC 2 cut(s) 30, 810
Tru1I TTAA 5 cut(s) 266, 510, 515, 834, 841
Tru9I TTAA 5 cut(s) 266, 510, 515, 834, 841
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 2 cut(s) 131, 152
Tsp45I GTSAC 1 cut(s) 409
TspRI CASTG 1 cut(s) 214
VspI ATTAAT 1 cut(s) 834
XapI RAATTY 3 cut(s) 252, 298, 440
XmnI GAANNNNTTC 1 cut(s) 809
XspI CTAG 3 cut(s) 72, 501, 740
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.