Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0012115

Physical Location & Seq

Genomic Coordinates Forward (+)

11920584 .. 11924778

Gene Structure 4195 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g20200.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGGCTAGTAATTTTGATACAGTGAGAGGAACCCCACCTGGGATTTTGTTGATTAGAAGCGTTAGAGGAAAAGATTGGAGGTTTTCTACATATAGATATGTGGTTTTGTTTCTTACATTTGTAGCTTATGCTAGTTACCATGCTTCAAGAAAACCCAGTAGTATAGTTAAGAGTGTTTTGTATCCTGAACCCACCAATAAGGACAATGGCTTGGTCCTCCCCTGGCTTGGCCAGGTGTTTGTTAAGACAAATTATATGGGGAATGCGAGTCATCATCGTCATAGGTTGATAGACAAGGGCTGGGCTCCTTTTAATAGCAAGGACGGGACGACGAAGCTAGGTGAGATTGATGTTGCATTTCTAGCATGTTACTCTCTAGGAATGTATGTTGCTGGGCATTTGGGCGATACGCTGGACCTTCGGCTGTTCTTGACATGCGGGATGGTTGGGAGTGGTGTTTTCGTTGCGCTATTTGGGATGGGGTACTTTTGGAACATTCATGTGTTTGGATTCTATCTTGGAATGCAAATGGTTGCAGGGTTGTTCCAAGCAACAGGTTGGCCTTCTGTTGTTGCGGTGATTGGGAATTGGTTTGGAAAGGGGAAGAGGGGTTTGATAATGGGGATATGGAATGCACATACTTCAGTTGGGAATATTAGTGGCTCGCTGCTTGCTGCTGGCGTTCTGGACTATGGTTGGGGTTGGTGTTTTATAGTTCCTGGTGCGTTTATTGCATTGGCAGGAATACTAGTTTTCTTGTTCTTGCCTGCATATCCTGAGGATATTGGATTTTCTAGTCCACACGGTTCAGCTTCAAGTTTGGAGGCAGTTCCTGCTGATGAAGAAGCAAATATGGGAAAAGGAATTCAGGTGGAAAGTGAAACAAATATGTCAAGAGCTGGGTCTGTGACAAGGACAGGTGTTGGTCTTATTGAAGCTTGTGCCATACCAGGAGTTATACCCTTTGCGTTATGCCTCTTCTTTGCAAAGCTTGTAGCCTACACATTTCTGTACTGGCTACCGTTTTATTTAAGTCAGACAGAAATTGGAGGAGAGTATGTTTATGTGAAATCTGCTGGTAATCTCTCTACTTTGTTTGATGTTGGGGGCATAGTTGGTGGAATCCTTGCAGGCTACATATCCGATAAACTTAATGCTCGAGCTACTACAGCAGCCTGCTTCATGTATACTGCAATTCCAGCAATGCTTTTGTATCGCAGATATGGGAGTATTTCACACAATATCAACATTTTGCTTATGATGGTTGCTGGATTGTTTATTAATGGGCCCTATGCACTTATCACTACTGCAGTTTCTGCAGACCTTGGCACACACAGTTCGCTTAGAGGTGACTCTAGAGCACTGGCAACAGTTACTGCCATCATTGATGGTACTGGATCAGTTGGTGCTGCTTTCGGTCCTCTTCTTACTGGTTTCCTTTCAACAATAGGATGGGATGCAGTATTTGTGATGCTAATGGTTTGTGCTCTTATAGCGGGGCTTCTGCTATCACGTTTGGTTATCTCTGAAATTTCAGAGAAGACTTGCAAACCGCCTGTATCCAGTGGGCGACACAATTTTGGAGCTCCTGTGTACCAACCACTTTTAAGTGATCAACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0008150 GO:0016020 GO:0031090 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0072505 GO:0072506 GO:0098588 GO:0098771 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1

Protein Analysis

541

Amino Acids

57.98

Weight (kDa)

8.64

Isoelectric Point (pI)

34.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	114 - 443	8.9e-34	Major Facilitator Superfamily
MFS_1	PF07690	358 - 521	2.1e-12	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012115)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17550
fragaria_vesca	FvH4_5g20200
malus_domestica	MD04G1051000.v1.1 MD04G1051400.v1.1 MD06G1048800.v1.1
prunus_persica	Prupe.5G059000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0205141
rosa_laevigata	RLG00000003396
rosa_multiflora	Rmu_sc0001072.1_g000004 Rmu_sc0009349.1_g000013
rosa_roxburghii	Rroxscaffold_3G00252550
rosa_rugosa	Rorug07G0088200 Rorug07G0088300 Rorug07G0088400
rosa_samantha	Rh7AG219300 Rh7BG215700 Rh7CG232300 Rh7DG226600
rosa_wichuraiana	Rw7G018970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1187
AciI	CCGC	4 cut(s)	438, 575, 1496, 1553
AclWI	GGATC	1 cut(s)	1405
AcoI	YGGCCR	1 cut(s)	229
AcsI	RAATTY	2 cut(s)	864, 1530
AcuI	CTGAAG	1 cut(s)	627
AfaI	GTAC	4 cut(s)	485, 1013, 1393, 1595
AfiI	CCNNNNNNNGG	2 cut(s)	39, 227
AgsI	TTSAA	4 cut(s)	147, 816, 935, 1443
AhlI	ACTAGT	1 cut(s)	748
AjnI	CCWGG	5 cut(s)	37, 221, 231, 718, 949
AluBI	AGCT	8 cut(s)	125, 337, 812, 899, 938, 991, 1163, 1586
AluI	AGCT	8 cut(s)	125, 337, 812, 899, 938, 991, 1163, 1586
Alw21I	GWGCWC	3 cut(s)	1363, 1489, 1588
AlwI	GGATC	1 cut(s)	1405
AlwNI	CAGNNNCTG	3 cut(s)	833, 1316, 1376
Ama87I	CYCGRG	1 cut(s)	1158
AoxI	GGCC	3 cut(s)	229, 560, 1286
ApaI	GGGCCC	1 cut(s)	1290
ApeKI	GCWGC	4 cut(s)	667, 674, 1172, 1409
ApoI	RAATTY	2 cut(s)	864, 1530
AseI	ATTAAT	1 cut(s)	1281
AspLEI	GCGC	1 cut(s)	469
AspS9I	GGNCC	5 cut(s)	214, 415, 1286, 1287, 1418
AsuHPI	GGTGA	3 cut(s)	353, 589, 1361
AvaI	CYCGRG	1 cut(s)	1158
AvaII	GGWCC	3 cut(s)	214, 415, 1418
AxyI	CCTNAGG	1 cut(s)	777
BaeGI	GKGCMC	1 cut(s)	1290
BalI	TGGCCA	1 cut(s)	231
BanII	GRGCYC	3 cut(s)	307, 1290, 1588
BbsI	GAAGAC	1 cut(s)	1547
Bbv12I	GWGCWC	3 cut(s)	1363, 1489, 1588
BbvI	GCAGC	4 cut(s)	654, 661, 1184, 1396
BccI	CCATC	6 cut(s)	436, 472, 1255, 1382, 1388, 1446
BciT130I	CCWGG	5 cut(s)	39, 223, 233, 720, 951
BciVI	GTATCC	2 cut(s)	192, 1570
BclI	TGATCA	1 cut(s)	1612
BcuI	ACTAGT	1 cut(s)	748
BfaI	CTAG	8 cut(s)	6, 132, 338, 362, 377, 749, 795, 1356
BfmI	CTRYAG	3 cut(s)	1167, 1308, 1317
BfuI	GTATCC	2 cut(s)	192, 1570
BisI	GCNGC	4 cut(s)	668, 675, 1173, 1410
BlsI	GCNGC	4 cut(s)	669, 676, 1174, 1411
Bme1390I	CCNGG	5 cut(s)	39, 223, 233, 720, 951
Bme18I	GGWCC	3 cut(s)	214, 415, 1418
BmeT110I	CYCGRG	1 cut(s)	1158
BmgT120I	GGNCC	5 cut(s)	214, 415, 1286, 1287, 1418
BmiI	GGNNCC	3 cut(s)	31, 306, 1288
BmrFI	CCNGG	5 cut(s)	39, 223, 233, 720, 951
BmrI	ACTGGG	1 cut(s)	150
BmsI	GCATC	2 cut(s)	1447, 1461
BmuI	ACTGGG	1 cut(s)	150
BpiI	GAAGAC	1 cut(s)	1547
BsaJI	CCNNGG	3 cut(s)	38, 221, 1324
BsaXI	ACNNNNNCTCC	4 cut(s)	1044, 1074, 1575, 1605
Bsc4I	CCNNNNNNNGG	2 cut(s)	39, 227
Bse1I	ACTGG	6 cut(s)	156, 1019, 1368, 1399, 1435, 1563
Bse21I	CCTNAGG	1 cut(s)	777
Bse3DI	GCAATG	1 cut(s)	1209
BseBI	CCWGG	5 cut(s)	39, 223, 233, 720, 951
BseDI	CCNNGG	3 cut(s)	38, 221, 1324
BseGI	GGATG	4 cut(s)	447, 483, 1457, 1462
BseLI	CCNNNNNNNGG	2 cut(s)	39, 227
BseMI	GCAATG	1 cut(s)	1209
BseMII	CTCAG	1 cut(s)	768
BseNI	ACTGG	6 cut(s)	156, 1019, 1368, 1399, 1435, 1563
BseRI	GAGGAG	1 cut(s)	1065
BseSI	GKGCMC	1 cut(s)	1290
BseXI	GCAGC	4 cut(s)	654, 661, 1184, 1396
BseYI	CCCAGC	3 cut(s)	300, 392, 899
BshFI	GGCC	3 cut(s)	231, 562, 1288
BsiHKAI	GWGCWC	3 cut(s)	1363, 1489, 1588
BsiHKCI	CYCGRG	1 cut(s)	1158
BslFI	GGGAC	1 cut(s)	340
BslI	CCNNNNNNNGG	2 cut(s)	39, 227
BsmFI	GGGAC	1 cut(s)	340
BsmI	GAATGC	3 cut(s)	268, 528, 637
BsnI	GGCC	3 cut(s)	231, 562, 1288
BsoBI	CYCGRG	1 cut(s)	1158
Bsp120I	GGGCCC	1 cut(s)	1286
Bsp1286I	GDGCHC	5 cut(s)	307, 1290, 1363, 1489, 1588
Bsp143I	GATC	2 cut(s)	1397, 1612
BspACI	CCGC	4 cut(s)	438, 575, 1496, 1553
BspANI	GGCC	3 cut(s)	231, 562, 1288
BspCNI	CTCAG	1 cut(s)	769
BspLI	GGNNCC	3 cut(s)	31, 306, 1288
BspMAI	CTGCAG	2 cut(s)	1312, 1321
BspPI	GGATC	1 cut(s)	1405
BsrDI	GCAATG	1 cut(s)	1209
BsrI	ACTGG	6 cut(s)	156, 1019, 1368, 1399, 1435, 1563
BssECI	CCNNGG	3 cut(s)	38, 221, 1324
BssMI	GATC	2 cut(s)	1397, 1612
BssNAI	GTATAC	1 cut(s)	1188
BssT1I	CCWWGG	1 cut(s)	1324
Bst1107I	GTATAC	1 cut(s)	1188
Bst2UI	CCWGG	5 cut(s)	39, 223, 233, 720, 951
Bst4CI	ACNGT	5 cut(s)	22, 806, 1023, 1337, 1372
Bst6I	CTCTTC	3 cut(s)	599, 983, 1428
BstAPI	GCANNNNNTGC	2 cut(s)	833, 1316
BstC8I	GCNNGC	6 cut(s)	665, 672, 679, 768, 1132, 1177
BstDEI	CTNAG	2 cut(s)	777, 1343
BstF5I	GGATG	4 cut(s)	447, 483, 1457, 1462
BstHHI	GCGC	1 cut(s)	469
BstKTI	GATC	2 cut(s)	1400, 1615
BstMBI	GATC	2 cut(s)	1397, 1612
BstMWI	GCNNNNNNNGC	7 cut(s)	362, 731, 833, 1169, 1199, 1316, 1493
BstNI	CCWGG	5 cut(s)	39, 223, 233, 720, 951
BstNSI	RCATGY	2 cut(s)	369, 438
BstSCI	CCNGG	5 cut(s)	37, 221, 231, 718, 949
BstSFI	CTRYAG	3 cut(s)	1167, 1308, 1317
BstSLI	GKGCMC	1 cut(s)	1290
BstV1I	GCAGC	4 cut(s)	654, 661, 1184, 1396
BstV2I	GAAGAC	1 cut(s)	1547
BstZ17I	GTATAC	1 cut(s)	1188
Bsu36I	CCTNAGG	1 cut(s)	777
BsuI	GTATCC	2 cut(s)	192, 1570
BsuRI	GGCC	3 cut(s)	231, 562, 1288
BtsCI	GGATG	4 cut(s)	447, 483, 1457, 1462
BtsIMutI	CAGTG	3 cut(s)	27, 1361, 1570
Cac8I	GCNNGC	6 cut(s)	665, 672, 679, 768, 1132, 1177
CaiI	CAGNNNCTG	3 cut(s)	833, 1316, 1376
CfoI	GCGC	1 cut(s)	469
Cfr13I	GGNCC	5 cut(s)	214, 415, 1286, 1287, 1418
Csp6I	GTAC	4 cut(s)	484, 1012, 1392, 1594
CviAII	CATG	5 cut(s)	140, 366, 435, 500, 1183
CviQI	GTAC	4 cut(s)	484, 1012, 1392, 1594
DdeI	CTNAG	2 cut(s)	777, 1343
DpnI	GATC	2 cut(s)	1399, 1614
DpnII	GATC	2 cut(s)	1397, 1612
EaeI	YGGCCR	1 cut(s)	229
Eam1104I	CTCTTC	3 cut(s)	599, 983, 1428
EarI	CTCTTC	3 cut(s)	599, 983, 1428
Ecl136II	GAGCTC	1 cut(s)	1586
Eco130I	CCWWGG	1 cut(s)	1324
Eco24I	GRGCYC	3 cut(s)	307, 1290, 1588
Eco47I	GGWCC	3 cut(s)	214, 415, 1418
Eco53kI	GAGCTC	1 cut(s)	1586
Eco57I	CTGAAG	1 cut(s)	627
Eco81I	CCTNAGG	1 cut(s)	777
Eco88I	CYCGRG	1 cut(s)	1158
EcoICRI	GAGCTC	1 cut(s)	1586
EcoO109I	RGGNCCY	1 cut(s)	1287
EcoRI	GAATTC	1 cut(s)	864
EcoRII	CCWGG	5 cut(s)	37, 221, 231, 718, 949
EcoT14I	CCWWGG	1 cut(s)	1324
EcoT38I	GRGCYC	3 cut(s)	307, 1290, 1588
ErhI	CCWWGG	1 cut(s)	1324
FaeI	CATG	5 cut(s)	143, 369, 438, 503, 1186
FaqI	GGGAC	1 cut(s)	340
FatI	CATG	5 cut(s)	139, 365, 434, 499, 1182
FauI	CCCGC	2 cut(s)	431, 1489
FbaI	TGATCA	1 cut(s)	1612
FblI	GTMKAC	1 cut(s)	1187
Fnu4HI	GCNGC	4 cut(s)	668, 675, 1173, 1410
FokI	GGATG	4 cut(s)	454, 490, 1464, 1469
FriOI	GRGCYC	3 cut(s)	307, 1290, 1588
Fsp4HI	GCNGC	4 cut(s)	668, 675, 1173, 1410
FspBI	CTAG	8 cut(s)	6, 132, 338, 362, 377, 749, 795, 1356
GlaI	GCGC	1 cut(s)	468
GluI	GCNGC	4 cut(s)	668, 675, 1173, 1410
GsaI	CCCAGC	3 cut(s)	304, 396, 903
HaeIII	GGCC	3 cut(s)	231, 562, 1288
HhaI	GCGC	1 cut(s)	469
Hin1II	CATG	5 cut(s)	143, 369, 438, 503, 1186
Hin6I	GCGC	1 cut(s)	467
HinP1I	GCGC	1 cut(s)	467
HindIII	AAGCTT	2 cut(s)	936, 989
HinfI	GANTC	4 cut(s)	268, 510, 1122, 1352
HphI	GGTGA	3 cut(s)	353, 589, 1361
Hpy166II	GTNNAC	3 cut(s)	800, 1188, 1594
Hpy188I	TCNGA	4 cut(s)	1038, 1144, 1528, 1537
Hpy188III	TCNNGA	7 cut(s)	147, 185, 430, 686, 776, 894, 1356
Hpy8I	GTNNAC	3 cut(s)	800, 1188, 1594
Hpy99I	CGWCG	1 cut(s)	334
HpyAV	CCTTC	2 cut(s)	428, 573
HpyCH4III	ACNGT	5 cut(s)	22, 806, 1023, 1337, 1372
HpyCH4IV	ACGT	1 cut(s)	1513
HpyF10VI	GCNNNNNNNGC	7 cut(s)	362, 731, 833, 1169, 1199, 1316, 1493
HpyF3I	CTNAG	2 cut(s)	777, 1343
HpySE526I	ACGT	1 cut(s)	1513
Hsp92II	CATG	5 cut(s)	143, 369, 438, 503, 1186
HspAI	GCGC	1 cut(s)	467
Ksp22I	TGATCA	1 cut(s)	1612
Kzo9I	GATC	2 cut(s)	1397, 1612
LmnI	GCTCC	3 cut(s)	310, 1583, 1591
Lsp1109I	GCAGC	4 cut(s)	654, 661, 1184, 1396
LweI	GCATC	2 cut(s)	1447, 1461
MaeI	CTAG	8 cut(s)	6, 132, 338, 362, 377, 749, 795, 1356
MaeII	ACGT	1 cut(s)	1513
MaeIII	GTNAC	5 cut(s)	134, 368, 907, 1349, 1372
MalI	GATC	2 cut(s)	1399, 1614
MboI	GATC	2 cut(s)	1397, 1612
MboII	GAAGA	5 cut(s)	616, 854, 970, 1415, 1552
MhlI	GDGCHC	5 cut(s)	307, 1290, 1363, 1489, 1588
MlsI	TGGCCA	1 cut(s)	231
MluCI	AATT	8 cut(s)	10, 250, 586, 864, 1044, 1194, 1530, 1576
MluNI	TGGCCA	1 cut(s)	231
MlyI	GAGTC	2 cut(s)	277, 1346
Mox20I	TGGCCA	1 cut(s)	231
MscI	TGGCCA	1 cut(s)	231
MseI	TTAA	7 cut(s)	168, 243, 312, 1031, 1152, 1281, 1607
MslI	CAYNNNNRTG	1 cut(s)	500
Msp20I	TGGCCA	1 cut(s)	231
MspR9I	CCNGG	5 cut(s)	39, 223, 233, 720, 951
Mva1269I	GAATGC	3 cut(s)	268, 528, 637
MvaI	CCWGG	5 cut(s)	39, 223, 233, 720, 951
MwoI	GCNNNNNNNGC	7 cut(s)	362, 731, 833, 1169, 1199, 1316, 1493
NdeII	GATC	2 cut(s)	1397, 1612
NlaIII	CATG	5 cut(s)	143, 369, 438, 503, 1186
NlaIV	GGNNCC	3 cut(s)	31, 306, 1288
NmuCI	GTSAC	2 cut(s)	907, 1349
NspI	RCATGY	2 cut(s)	369, 438
PaeR7I	CTCGAG	1 cut(s)	1158
PctI	GAATGC	3 cut(s)	268, 528, 637
PfeI	GAWTC	2 cut(s)	510, 1122
PkrI	GCNGC	4 cut(s)	669, 676, 1174, 1411
PleI	GAGTC	2 cut(s)	276, 1346
PpsI	GAGTC	2 cut(s)	276, 1346
PshBI	ATTAAT	1 cut(s)	1281
Psp124BI	GAGCTC	1 cut(s)	1588
Psp6I	CCWGG	5 cut(s)	37, 221, 231, 718, 949
PspFI	CCCAGC	3 cut(s)	300, 392, 899
PspGI	CCWGG	5 cut(s)	37, 221, 231, 718, 949
PspN4I	GGNNCC	3 cut(s)	31, 306, 1288
PspOMI	GGGCCC	1 cut(s)	1286
PspPI	GGNCC	5 cut(s)	214, 415, 1286, 1287, 1418
PspXI	VCTCGAGB	1 cut(s)	1158
PstI	CTGCAG	2 cut(s)	1312, 1321
PstNI	CAGNNNCTG	3 cut(s)	833, 1316, 1376
RsaI	GTAC	4 cut(s)	485, 1013, 1393, 1595
RsaNI	GTAC	4 cut(s)	484, 1012, 1392, 1594
RseI	CAYNNNNRTG	1 cut(s)	500
SacI	GAGCTC	1 cut(s)	1588
SaqAI	TTAA	7 cut(s)	168, 243, 312, 1031, 1152, 1281, 1607
SatI	GCNGC	4 cut(s)	668, 675, 1173, 1410
Sau3AI	GATC	2 cut(s)	1397, 1612
Sau96I	GGNCC	5 cut(s)	214, 415, 1286, 1287, 1418
SchI	GAGTC	2 cut(s)	277, 1346
ScrFI	CCNGG	5 cut(s)	39, 223, 233, 720, 951
SduI	GDGCHC	5 cut(s)	307, 1290, 1363, 1489, 1588
SfaNI	GCATC	2 cut(s)	1447, 1461
SfcI	CTRYAG	3 cut(s)	1167, 1308, 1317
Sfr274I	CTCGAG	1 cut(s)	1158
SinI	GGWCC	3 cut(s)	214, 415, 1418
SlaI	CTCGAG	1 cut(s)	1158
SmiMI	CAYNNNNRTG	1 cut(s)	500
SmlI	CTYRAG	1 cut(s)	1158
SmoI	CTYRAG	1 cut(s)	1158
SpeI	ACTAGT	1 cut(s)	748
Sse9I	AATT	8 cut(s)	10, 250, 586, 864, 1044, 1194, 1530, 1576
SsiI	CCGC	4 cut(s)	438, 575, 1496, 1553
SspI	AATATT	1 cut(s)	655
SspMI	CTAG	8 cut(s)	6, 132, 338, 362, 377, 749, 795, 1356
SstI	GAGCTC	1 cut(s)	1588
StyD4I	CCNGG	5 cut(s)	37, 221, 231, 718, 949
StyI	CCWWGG	1 cut(s)	1324
TaaI	ACNGT	5 cut(s)	22, 806, 1023, 1337, 1372
TaiI	ACGT	1 cut(s)	1516
TaqI	TCGA	1 cut(s)	1159
TaqII	GACCGA	1 cut(s)	1406
TasI	AATT	8 cut(s)	10, 250, 586, 864, 1044, 1194, 1530, 1576
TatI	WGTACW	1 cut(s)	1011
TfiI	GAWTC	2 cut(s)	510, 1122
Tru1I	TTAA	7 cut(s)	168, 243, 312, 1031, 1152, 1281, 1607
Tru9I	TTAA	7 cut(s)	168, 243, 312, 1031, 1152, 1281, 1607
TscAI	CASTG	3 cut(s)	27, 1368, 1570
TseFI	GTSAC	2 cut(s)	907, 1349
TseI	GCWGC	4 cut(s)	667, 674, 1172, 1409
Tsp45I	GTSAC	2 cut(s)	907, 1349
TspDTI	ATGAA	3 cut(s)	488, 855, 1171
TspRI	CASTG	3 cut(s)	27, 1368, 1570
VpaK11BI	GGWCC	3 cut(s)	214, 415, 1418
VspI	ATTAAT	1 cut(s)	1281
XapI	RAATTY	2 cut(s)	864, 1530
XbaI	TCTAGA	1 cut(s)	1355
XceI	RCATGY	2 cut(s)	369, 438
XhoI	CTCGAG	1 cut(s)	1158
XmiI	GTMKAC	1 cut(s)	1187
XspI	CTAG	8 cut(s)	6, 132, 338, 362, 377, 749, 795, 1356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.