Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0012115

Physical Location & Seq

Genomic Coordinates Forward (+)

6574109 .. 6577898

Gene Structure 3790 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1048800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGTCTCAGAACTCTGATACAATGAGAGGAATCCCACCTGGGATTTTGCTGATTAGAAGCATTAGAGGCAAAGATTGGAGTCTTTCTACATATAGATATGTGATTTTGCTTGTTACTTTCATAGCTTATACATGTTACCATGCATCTAGAAAGCCCAGTAGTATAGTTAAAAGTGTTTTGTATCCTGACCCCAATAAAGGCAATGGCTTGGACCCCTTGTTTGGCAAAGTCTTTGTCAAGAGAGAGTTTGGGGGCAATGAGAATTATAGGATAGACAAGGGTTGGGCTCCCTTCAACGGAACCGATGGGACGTCGAAATTGGGGGAGATTGATCTTGCATTTCTTGCATGTTACTCTTTGGGAATGTATGTTGCTGGGCATTTGGGAGATACTTTGGACCTCCGGTTGTTCTTGACGACCGGGATGATTGGGAGTGGCATTTTTGTTGCCCTGTTTGGGATGGGATACTTTTGGAATATCCATGTGTTTGGTTACTATCTTGGGATGCAAATGATTGCCGGGTTGTTCCAGGCAACGGGTTGGCCTTCGGTTGTTGCTGTGATTGGTAATTGGTTTGGAAAGGGTAAGAGGGGTTTGATAATGGGCATATGGAATGCGCATACTTCGGTTGGGAATATAAGTGGGTCACTTCTTGCTGCTAGTGTTCTGGATTATGGGTGGGGTTGGTGCTTTATTGTTCCAGGTGTTTTTATAGCAGTTGGAGGCGTAATCGTTTTCTTCTTCTTGGCTGCTTATCCTGAAGATATTGGATTTTCTTTCCCGCCAAATTCAGCTCCTAATTTGGAGGCAACGGCCGATGACGAAGAAGCTAATATAAAAAAAGGATTTATTGTCGAGGGTGAAACAAAAACTGTTCGCCGAATTGGGTCTGTGAGTAGGACAAGTATTGGACTTATTCAAGCTTGTACAATACCAGGAGTTATACCTTTTGCGTTGTGCCTCTTCTTCTCAAAGCTTGTGGCCTACACATTTCTGTACTGGCTACCATTTTATCTCAGTCAGACAGAAATAGGGGGAGAGTATGTTTCTGTGAAATCTGCTGGAAATCTCTCCACTTTGTTCGATGTGGGAGGCATAGTTGGTGGAATCCTTGCTGGCTACATATCTGATAAACTTAATGCTCGAGCTACCACGGCAGCAGGCTTCATGTCCTTTGCAATTCCGGCAATGCTTTTATATCGGAAATATGGCAGCATTTCACACAATGCCAACATTGCACTTATGATGGTTGCTGGCTTGTTTATTAATGGGCCCTATGCACTTATCACCACTGCAGTTTCTGCAGACCTTGGTACGCACAGCTCCCTTAGAGGGGATTCTCGAGCACTGGCAACAGTTACCGCCATAATTGATGGTACAGGATCAGTTGGTGCAGCTTTTGGCCCTCTTCTTACTGGTTTCCTTTCAACAAAGGGATGGGATGCAGTATTTGTGATGTTAATGATTTGTGCTCTTATTGCGACGCTTCTTTTATCACGTTTGGTTATAGCTGAAATTTCTGAGAAGATGTGCAAACCAGTACCTGTATCCAGTGAGCAAGGTTTTGGAGCTCCTGCATCACAACCACTTCTAAGTGATCAACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0008150 GO:0016020 GO:0031090 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0072505 GO:0072506 GO:0098588 GO:0098771 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1

Protein Analysis

536

Amino Acids

57.33

Weight (kDa)

8.52

Isoelectric Point (pI)

29.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	110 - 344	3.1e-26	Major Facilitator Superfamily
MFS_1	PF07690	319 - 502	1e-12	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012115)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17550
fragaria_vesca	FvH4_5g20200
malus_domestica	MD04G1051000.v1.1 MD04G1051400.v1.1 MD06G1048800.v1.1
prunus_persica	Prupe.5G059000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0205141
rosa_laevigata	RLG00000003396
rosa_multiflora	Rmu_sc0001072.1_g000004 Rmu_sc0009349.1_g000013
rosa_roxburghii	Rroxscaffold_3G00252550
rosa_rugosa	Rorug07G0088200 Rorug07G0088300 Rorug07G0088400
rosa_samantha	Rh7AG219300 Rh7BG215700 Rh7CG232300 Rh7DG226600
rosa_wichuraiana	Rw7G018970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	314
Acc16I	TGCGCA	1 cut(s)	618
AciI	CCGC	2 cut(s)	782, 1362
AclWI	GGATC	1 cut(s)	1390
AcoI	YGGCCR	1 cut(s)	813
AcsI	RAATTY	2 cut(s)	787, 1515
AcuI	CTGAAG	1 cut(s)	780
AcyI	GRCGYC	1 cut(s)	311
AfaI	GTAC	5 cut(s)	928, 998, 1315, 1378, 1542
AfiI	CCNNNNNNNGG	7 cut(s)	197, 221, 296, 456, 535, 1332, 1333
AflIII	ACRYGT	1 cut(s)	131
AgsI	TTSAA	3 cut(s)	295, 920, 1428
AjnI	CCWGG	4 cut(s)	37, 528, 700, 934
Alw21I	GWGCWC	3 cut(s)	1348, 1474, 1573
Alw26I	GTCTC	1 cut(s)	9
AlwI	GGATC	1 cut(s)	1390
AlwNI	CAGNNNCTG	2 cut(s)	1301, 1544
Ama87I	CYCGRG	2 cut(s)	1143, 1341
AoxI	GGCC	5 cut(s)	542, 813, 981, 1271, 1402
ApaI	GGGCCC	1 cut(s)	1275
ApeKI	GCWGC	5 cut(s)	656, 749, 1157, 1212, 1394
ApoI	RAATTY	2 cut(s)	787, 1515
ArsI	GACNNNNNNTTYG	4 cut(s)	203, 219, 235, 251
AseI	ATTAAT	1 cut(s)	1266
AspLEI	GCGC	1 cut(s)	619
AspS9I	GGNCC	5 cut(s)	211, 397, 1271, 1272, 1403
AsuC2I	CCSGG	2 cut(s)	421, 520
AsuHPI	GGTGA	2 cut(s)	872, 1279
AvaI	CYCGRG	2 cut(s)	1143, 1341
AvaII	GGWCC	2 cut(s)	211, 397
BaeGI	GKGCMC	1 cut(s)	1275
BanII	GRGCYC	3 cut(s)	289, 1275, 1573
Bbv12I	GWGCWC	3 cut(s)	1348, 1474, 1573
BbvI	GCAGC	5 cut(s)	643, 736, 1169, 1224, 1406
BccI	CCATC	5 cut(s)	299, 454, 1240, 1367, 1431
BceAI	ACGGC	2 cut(s)	828, 1170
BciT130I	CCWGG	4 cut(s)	39, 530, 702, 936
BciVI	GTATCC	3 cut(s)	192, 458, 1558
BclI	TGATCA	1 cut(s)	1597
BcnI	CCSGG	2 cut(s)	421, 520
BcoDI	GTCTC	1 cut(s)	9
BfaI	CTAG	2 cut(s)	147, 660
BfmI	CTRYAG	2 cut(s)	1293, 1302
BfuI	GTATCC	3 cut(s)	192, 458, 1558
BisI	GCNGC	5 cut(s)	657, 750, 1158, 1213, 1395
BlsI	GCNGC	5 cut(s)	658, 751, 1159, 1214, 1396
Bme1390I	CCNGG	6 cut(s)	39, 421, 520, 530, 702, 936
Bme18I	GGWCC	2 cut(s)	211, 397
BmeT110I	CYCGRG	2 cut(s)	1143, 1341
BmgT120I	GGNCC	5 cut(s)	211, 397, 1271, 1272, 1403
BmiI	GGNNCC	4 cut(s)	213, 288, 301, 1273
BmrFI	CCNGG	6 cut(s)	39, 421, 520, 530, 702, 936
BmrI	ACTGGG	1 cut(s)	150
BmsI	GCATC	4 cut(s)	152, 495, 1432, 1586
BmuI	ACTGGG	1 cut(s)	150
BpuMI	CCSGG	2 cut(s)	421, 520
BsaHI	GRCGYC	1 cut(s)	311
BsaJI	CCNNGG	3 cut(s)	38, 1152, 1309
BsaWI	WCCGGW	1 cut(s)	402
BsaXI	ACNNNNNCTCC	4 cut(s)	1029, 1059, 1083, 1113
Bsc4I	CCNNNNNNNGG	7 cut(s)	197, 221, 296, 456, 535, 1332, 1333
Bse1I	ACTGG	6 cut(s)	156, 1004, 1353, 1420, 1538, 1551
Bse3DI	GCAATG	4 cut(s)	208, 262, 1194, 1233
BseBI	CCWGG	4 cut(s)	39, 530, 702, 936
BseDI	CCNNGG	3 cut(s)	38, 1152, 1309
BseGI	GGATG	5 cut(s)	429, 465, 510, 1442, 1447
BseLI	CCNNNNNNNGG	7 cut(s)	197, 221, 296, 456, 535, 1332, 1333
BseMI	GCAATG	4 cut(s)	208, 262, 1194, 1233
BseMII	CTCAG	3 cut(s)	20, 1030, 1512
BseNI	ACTGG	6 cut(s)	156, 1004, 1353, 1420, 1538, 1551
BseSI	GKGCMC	1 cut(s)	1275
BseX3I	CGGCCG	1 cut(s)	813
BseXI	GCAGC	5 cut(s)	643, 736, 1169, 1224, 1406
BseYI	CCCAGC	1 cut(s)	374
BsgI	GTGCAG	1 cut(s)	1413
Bsh1285I	CGRYCG	2 cut(s)	420, 816
BshFI	GGCC	5 cut(s)	544, 815, 983, 1273, 1404
BsiEI	CGRYCG	2 cut(s)	420, 816
BsiHKAI	GWGCWC	3 cut(s)	1348, 1474, 1573
BsiHKCI	CYCGRG	2 cut(s)	1143, 1341
BsiSI	CCGG	4 cut(s)	403, 420, 519, 1184
BslFI	GGGAC	1 cut(s)	322
BslI	CCNNNNNNNGG	7 cut(s)	197, 221, 296, 456, 535, 1332, 1333
BsmAI	GTCTC	1 cut(s)	9
BsmFI	GGGAC	1 cut(s)	322
BsmI	GAATGC	1 cut(s)	619
BsnI	GGCC	5 cut(s)	544, 815, 983, 1273, 1404
BsoBI	CYCGRG	2 cut(s)	1143, 1341
Bsp120I	GGGCCC	1 cut(s)	1271
Bsp1286I	GDGCHC	5 cut(s)	289, 1275, 1348, 1474, 1573
Bsp1407I	TGTACA	1 cut(s)	926
Bsp143I	GATC	3 cut(s)	331, 1382, 1597
BspACI	CCGC	2 cut(s)	782, 1362
BspANI	GGCC	5 cut(s)	544, 815, 983, 1273, 1404
BspCNI	CTCAG	3 cut(s)	19, 1029, 1513
BspLI	GGNNCC	4 cut(s)	213, 288, 301, 1273
BspMAI	CTGCAG	2 cut(s)	1297, 1306
BspPI	GGATC	1 cut(s)	1390
BsrDI	GCAATG	4 cut(s)	208, 262, 1194, 1233
BsrGI	TGTACA	1 cut(s)	926
BsrI	ACTGG	6 cut(s)	156, 1004, 1353, 1420, 1538, 1551
BssECI	CCNNGG	3 cut(s)	38, 1152, 1309
BssMI	GATC	3 cut(s)	331, 1382, 1597
BssNI	GRCGYC	1 cut(s)	311
BssT1I	CCWWGG	1 cut(s)	1309
Bst2UI	CCWGG	4 cut(s)	39, 530, 702, 936
Bst4CI	ACNGT	2 cut(s)	874, 1357
Bst6I	CTCTTC	2 cut(s)	968, 1413
BstACI	GRCGYC	1 cut(s)	311
BstAPI	GCANNNNNTGC	2 cut(s)	344, 1301
BstAUI	TGTACA	1 cut(s)	926
BstC8I	GCNNGC	3 cut(s)	1117, 1162, 1255
BstDEI	CTNAG	5 cut(s)	6, 1016, 1328, 1521, 1592
BstDSI	CCRYGG	1 cut(s)	1152
BstF5I	GGATG	5 cut(s)	429, 465, 510, 1442, 1447
BstHHI	GCGC	1 cut(s)	619
BstKTI	GATC	3 cut(s)	334, 1385, 1600
BstMAI	GTCTC	1 cut(s)	9
BstMBI	GATC	3 cut(s)	331, 1382, 1597
BstMCI	CGRYCG	2 cut(s)	420, 816
BstMWI	GCNNNNNNNGC	7 cut(s)	66, 344, 1154, 1184, 1235, 1301, 1478
BstNI	CCWGG	4 cut(s)	39, 530, 702, 936
BstNSI	RCATGY	2 cut(s)	135, 351
BstSCI	CCNGG	6 cut(s)	37, 419, 518, 528, 700, 934
BstSFI	CTRYAG	2 cut(s)	1293, 1302
BstSLI	GKGCMC	1 cut(s)	1275
BstV1I	GCAGC	5 cut(s)	643, 736, 1169, 1224, 1406
BstXI	CCANNNNNNTGG	1 cut(s)	488
BstZI	CGGCCG	1 cut(s)	813
BsuI	GTATCC	3 cut(s)	192, 458, 1558
BsuRI	GGCC	5 cut(s)	544, 815, 983, 1273, 1404
BtgI	CCRYGG	1 cut(s)	1152
BtsCI	GGATG	5 cut(s)	429, 465, 510, 1442, 1447
BtsI	GCAGTG	1 cut(s)	1290
BtsIMutI	CAGTG	3 cut(s)	1290, 1346, 1558
Cac8I	GCNNGC	3 cut(s)	1117, 1162, 1255
CaiI	CAGNNNCTG	2 cut(s)	1301, 1544
CfoI	GCGC	1 cut(s)	619
Cfr13I	GGNCC	5 cut(s)	211, 397, 1271, 1272, 1403
CseI	GACGC	1 cut(s)	1492
Csp6I	GTAC	5 cut(s)	927, 997, 1314, 1377, 1541
CspCI	CAANNNNNGTGG	2 cut(s)	960, 995
CviAII	CATG	5 cut(s)	132, 140, 348, 482, 1168
CviQI	GTAC	5 cut(s)	927, 997, 1314, 1377, 1541
DdeI	CTNAG	5 cut(s)	6, 1016, 1328, 1521, 1592
DpnI	GATC	3 cut(s)	333, 1384, 1599
DpnII	GATC	3 cut(s)	331, 1382, 1597
EaeI	YGGCCR	1 cut(s)	813
EagI	CGGCCG	1 cut(s)	813
Eam1104I	CTCTTC	2 cut(s)	968, 1413
EarI	CTCTTC	2 cut(s)	968, 1413
Ecl136II	GAGCTC	1 cut(s)	1571
EclXI	CGGCCG	1 cut(s)	813
Eco130I	CCWWGG	1 cut(s)	1309
Eco24I	GRGCYC	3 cut(s)	289, 1275, 1573
Eco47I	GGWCC	2 cut(s)	211, 397
Eco52I	CGGCCG	1 cut(s)	813
Eco53kI	GAGCTC	1 cut(s)	1571
Eco57I	CTGAAG	1 cut(s)	780
Eco88I	CYCGRG	2 cut(s)	1143, 1341
EcoICRI	GAGCTC	1 cut(s)	1571
EcoO109I	RGGNCCY	1 cut(s)	1272
EcoRII	CCWGG	4 cut(s)	37, 528, 700, 934
EcoT14I	CCWWGG	1 cut(s)	1309
EcoT22I	ATGCAT	1 cut(s)	145
EcoT38I	GRGCYC	3 cut(s)	289, 1275, 1573
ErhI	CCWWGG	1 cut(s)	1309
FaeI	CATG	5 cut(s)	135, 143, 351, 485, 1171
FaqI	GGGAC	1 cut(s)	322
FatI	CATG	5 cut(s)	131, 139, 347, 481, 1167
FauI	CCCGC	1 cut(s)	789
FauNDI	CATATG	1 cut(s)	608
FbaI	TGATCA	1 cut(s)	1597
Fnu4HI	GCNGC	5 cut(s)	657, 750, 1158, 1213, 1395
FokI	GGATG	5 cut(s)	436, 472, 517, 1449, 1454
FriOI	GRGCYC	3 cut(s)	289, 1275, 1573
Fsp4HI	GCNGC	5 cut(s)	657, 750, 1158, 1213, 1395
FspAI	RTGCGCAY	1 cut(s)	618
FspBI	CTAG	2 cut(s)	147, 660
FspI	TGCGCA	1 cut(s)	618
GlaI	GCGC	1 cut(s)	618
GluI	GCNGC	5 cut(s)	657, 750, 1158, 1213, 1395
GsaI	CCCAGC	1 cut(s)	378
HaeIII	GGCC	5 cut(s)	544, 815, 983, 1273, 1404
HapII	CCGG	4 cut(s)	403, 420, 519, 1184
HgaI	GACGC	1 cut(s)	1492
HhaI	GCGC	1 cut(s)	619
Hin1I	GRCGYC	1 cut(s)	311
Hin1II	CATG	5 cut(s)	135, 143, 351, 485, 1171
Hin6I	GCGC	1 cut(s)	617
HinP1I	GCGC	1 cut(s)	617
HindIII	AAGCTT	2 cut(s)	921, 974
HinfI	GANTC	4 cut(s)	30, 79, 1107, 1337
HpaII	CCGG	4 cut(s)	403, 420, 519, 1184
HphI	GGTGA	2 cut(s)	872, 1279
Hpy188I	TCNGA	6 cut(s)	9, 16, 1023, 1129, 1203, 1522
Hpy188III	TCNNGA	7 cut(s)	147, 185, 238, 412, 668, 758, 1341
Hpy99I	CGWCG	2 cut(s)	316, 1486
HpyAV	CCTTC	2 cut(s)	301, 555
HpyCH4III	ACNGT	2 cut(s)	874, 1357
HpyCH4IV	ACGT	2 cut(s)	311, 1498
HpyF10VI	GCNNNNNNNGC	7 cut(s)	66, 344, 1154, 1184, 1235, 1301, 1478
HpyF3I	CTNAG	5 cut(s)	6, 1016, 1328, 1521, 1592
HpySE526I	ACGT	2 cut(s)	311, 1498
Hsp92I	GRCGYC	1 cut(s)	311
Hsp92II	CATG	5 cut(s)	135, 143, 351, 485, 1171
HspAI	GCGC	1 cut(s)	617
Ksp22I	TGATCA	1 cut(s)	1597
Kzo9I	GATC	3 cut(s)	331, 1382, 1597
LmnI	GCTCC	5 cut(s)	292, 799, 1328, 1568, 1576
Lsp1109I	GCAGC	5 cut(s)	643, 736, 1169, 1224, 1406
LweI	GCATC	4 cut(s)	152, 495, 1432, 1586
MaeI	CTAG	2 cut(s)	147, 660
MaeII	ACGT	2 cut(s)	311, 1498
MaeIII	GTNAC	6 cut(s)	112, 134, 350, 491, 645, 1357
MalI	GATC	3 cut(s)	333, 1384, 1599
MboI	GATC	3 cut(s)	331, 1382, 1597
MboII	GAAGA	8 cut(s)	730, 733, 773, 836, 955, 958, 1400, 1537
MhlI	GDGCHC	5 cut(s)	289, 1275, 1348, 1474, 1573
MluCI	AATT	9 cut(s)	262, 317, 568, 787, 799, 882, 1179, 1368, 1515
MlyI	GAGTC	1 cut(s)	88
MmeI	TCCRAC	1 cut(s)	700
Mph1103I	ATGCAT	1 cut(s)	145
MseI	TTAA	4 cut(s)	168, 1137, 1266, 1460
MspI	CCGG	4 cut(s)	403, 420, 519, 1184
MspR9I	CCNGG	6 cut(s)	39, 421, 520, 530, 702, 936
Mva1269I	GAATGC	1 cut(s)	619
MvaI	CCWGG	4 cut(s)	39, 530, 702, 936
MwoI	GCNNNNNNNGC	7 cut(s)	66, 344, 1154, 1184, 1235, 1301, 1478
NciI	CCSGG	2 cut(s)	421, 520
NdeI	CATATG	1 cut(s)	608
NdeII	GATC	3 cut(s)	331, 1382, 1597
NlaIII	CATG	5 cut(s)	135, 143, 351, 485, 1171
NlaIV	GGNNCC	4 cut(s)	213, 288, 301, 1273
NmuCI	GTSAC	1 cut(s)	645
NsbI	TGCGCA	1 cut(s)	618
NsiI	ATGCAT	1 cut(s)	145
NspI	RCATGY	2 cut(s)	135, 351
PaeR7I	CTCGAG	2 cut(s)	1143, 1341
PciI	ACATGT	1 cut(s)	131
PctI	GAATGC	1 cut(s)	619
PfeI	GAWTC	3 cut(s)	30, 1107, 1337
PkrI	GCNGC	5 cut(s)	658, 751, 1159, 1214, 1396
PleI	GAGTC	1 cut(s)	87
PpsI	GAGTC	1 cut(s)	87
PscI	ACATGT	1 cut(s)	131
PshBI	ATTAAT	1 cut(s)	1266
Psp124BI	GAGCTC	1 cut(s)	1573
Psp6I	CCWGG	4 cut(s)	37, 528, 700, 934
PspFI	CCCAGC	1 cut(s)	374
PspGI	CCWGG	4 cut(s)	37, 528, 700, 934
PspN4I	GGNNCC	4 cut(s)	213, 288, 301, 1273
PspOMI	GGGCCC	1 cut(s)	1271
PspPI	GGNCC	5 cut(s)	211, 397, 1271, 1272, 1403
PspXI	VCTCGAGB	1 cut(s)	1143
PstI	CTGCAG	2 cut(s)	1297, 1306
PstNI	CAGNNNCTG	2 cut(s)	1301, 1544
RsaI	GTAC	5 cut(s)	928, 998, 1315, 1378, 1542
RsaNI	GTAC	5 cut(s)	927, 997, 1314, 1377, 1541
SacI	GAGCTC	1 cut(s)	1573
SaqAI	TTAA	4 cut(s)	168, 1137, 1266, 1460
SatI	GCNGC	5 cut(s)	657, 750, 1158, 1213, 1395
Sau3AI	GATC	3 cut(s)	331, 1382, 1597
Sau96I	GGNCC	5 cut(s)	211, 397, 1271, 1272, 1403
SchI	GAGTC	1 cut(s)	88
ScrFI	CCNGG	6 cut(s)	39, 421, 520, 530, 702, 936
SduI	GDGCHC	5 cut(s)	289, 1275, 1348, 1474, 1573
SfaNI	GCATC	4 cut(s)	152, 495, 1432, 1586
SfcI	CTRYAG	2 cut(s)	1293, 1302
Sfr274I	CTCGAG	2 cut(s)	1143, 1341
SinI	GGWCC	2 cut(s)	211, 397
SlaI	CTCGAG	2 cut(s)	1143, 1341
SmlI	CTYRAG	2 cut(s)	1143, 1341
SmoI	CTYRAG	2 cut(s)	1143, 1341
Sse9I	AATT	9 cut(s)	262, 317, 568, 787, 799, 882, 1179, 1368, 1515
SsiI	CCGC	2 cut(s)	782, 1362
SspMI	CTAG	2 cut(s)	147, 660
SstI	GAGCTC	1 cut(s)	1573
StyD4I	CCNGG	6 cut(s)	37, 419, 518, 528, 700, 934
StyI	CCWWGG	1 cut(s)	1309
TaaI	ACNGT	2 cut(s)	874, 1357
TaiI	ACGT	2 cut(s)	314, 1501
TaqI	TCGA	5 cut(s)	314, 855, 1083, 1144, 1342
TasI	AATT	9 cut(s)	262, 317, 568, 787, 799, 882, 1179, 1368, 1515
TatI	WGTACW	2 cut(s)	926, 996
TfiI	GAWTC	3 cut(s)	30, 1107, 1337
Tru1I	TTAA	4 cut(s)	168, 1137, 1266, 1460
Tru9I	TTAA	4 cut(s)	168, 1137, 1266, 1460
TscAI	CASTG	3 cut(s)	1297, 1353, 1558
TseFI	GTSAC	1 cut(s)	645
TseI	GCWGC	5 cut(s)	656, 749, 1157, 1212, 1394
Tsp45I	GTSAC	1 cut(s)	645
TspDTI	ATGAA	2 cut(s)	109, 1156
TspGWI	ACGGA	1 cut(s)	312
TspRI	CASTG	3 cut(s)	1297, 1353, 1558
VpaK11BI	GGWCC	2 cut(s)	211, 397
VspI	ATTAAT	1 cut(s)	1266
XapI	RAATTY	2 cut(s)	787, 1515
XbaI	TCTAGA	1 cut(s)	146
XceI	RCATGY	2 cut(s)	135, 351
XhoI	CTCGAG	2 cut(s)	1143, 1341
XspI	CTAG	2 cut(s)	147, 660
ZraI	GACGTC	1 cut(s)	312
Zsp2I	ATGCAT	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.