Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0012115

Physical Location & Seq

Genomic Coordinates Reverse (-)

6019577 .. 6022594

Gene Structure 3018 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1051000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGACGGTTTTGCAAAATTGGAGTCTTTCTACATATAGATATCTAATTTTGCTTGTTACTTTCATAGCTTATACATGTTACCATGCATCTAGAAAACCCAGTAGTGTAGTTAAAAGTGTTTTGTATCCTGATCCCCATAGAGGCAATGGCTTGGACTCCTTGTTTGGTAAAGTCTTTCTCAAGAGAGAGTTTAGGGGCAATGAGAATTGTAGGTTACACAAGGGTTGGGCCCCCTTTAATGGAACCATCGGGATATCGAAATTGGGGGAGATTGATCTTGCGTTTCTTGCATGTTACTCTTTGGGAATGTATGTTGCTGGCCATTTGGGAGATACTTTGGACCTCCGATTGTTCTTGACGACCGGGATGTCTGGGAGTGGCATTTTTGTTGCCTTGTTTGGGATGGGATACTTTTTGAATATTCCTGTGTTTGGTTTCCATCTTGGGATGCAAATGATTGCCGGGTTGTTCCAAGCAACCGGTTGGCCTTCAGTTGTTGCTGTGATTGGTAATTGGTTTGGAAGGGGAAAGAGGGGTTTGATAATGGGCATATGGAATGCGCATACTTCGGTTGGGAATAAAAGTGGTTCACTTCTTGCTGCTAGTGTTCTAGATTATGGGTGGGGTTGGTGCTTTATTGTTCCAGCTTCTAATTTGGAGGCAGTACCTGATGATGAAGAAGTTAATATGAAAAAGGGATTCATGGTCGACGGTAAAATAAAAACTATATCCCGAATTGGGTCTGTGAGTAGGACAAGTATCGGAATTATTGAAGCTTGTATGATACCAGGAGTTATACCCTTTGCGTTGTGCTTCTTCTTGTCAAAGCTTGTGGCCTACACATTTCTGTACTGGCTACCATTTTATCTAACTGAGACAGAAATAGGAGGCAAGTATGTTTCTGTGAAATCTGCTGGAAACCTCTCCACTTTGTTCGATGTAGGAGGCATAGTTGGTGGAATCCTCGCTGGCTACATATATGATAAGCTTAATGCCCGAGCTACCACAGCAGCCGGCTTCATGTCATTTGCAATTCCGGCAATGCTATTATATCGAAAATACGGCAGCATTTCAAACAATGTCAATATTGCGCTTATGATGGTTGCTGGCTTGTTTATTAATGGACCCTATGCACTTATCACCACCGCAGTTTCTGCAAACCTTGGAACGCACAGTTCCCTTAGAGGGAATTCTCGAGCACTGGCAACGGTTACCACCATAATTGATGGTACTGGATCAGTTGGTGCAGCTTTTGGCCCTCTTCTTACTGGTTTCCTTTCAACAAAGGGATGGGATGCGGTATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0008150 GO:0016020 GO:0031090 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0072505 GO:0072506 GO:0098588 GO:0098771 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1

Protein Analysis

436

Amino Acids

46.8

Weight (kDa)

9.25

Isoelectric Point (pI)

24.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	89 - 216	5.5e-18	Major Facilitator Superfamily
MFS_1	PF07690	269 - 433	1.9e-08	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012115)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17550
fragaria_vesca	FvH4_5g20200
malus_domestica	MD04G1051000.v1.1 MD04G1051400.v1.1 MD06G1048800.v1.1
prunus_persica	Prupe.5G059000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0205141
rosa_laevigata	RLG00000003396
rosa_multiflora	Rmu_sc0001072.1_g000004 Rmu_sc0009349.1_g000013
rosa_roxburghii	Rroxscaffold_3G00252550
rosa_rugosa	Rorug07G0088200 Rorug07G0088300 Rorug07G0088400
rosa_samantha	Rh7AG219300 Rh7BG215700 Rh7CG232300 Rh7DG226600
rosa_wichuraiana	Rw7G018970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	367
Acc16I	TGCGCA	1 cut(s)	561
AccI	GTMKAC	1 cut(s)	708
AciI	CCGC	2 cut(s)	1146, 1298
AclWI	GGATC	2 cut(s)	125, 1243
AcoI	YGGCCR	1 cut(s)	319
AcsI	RAATTY	1 cut(s)	1189
AcuI	CTGAAG	1 cut(s)	474
AfaI	GTAC	3 cut(s)	666, 851, 1231
AfiI	CCNNNNNNNGG	6 cut(s)	140, 239, 399, 431, 738, 1185
AflIII	ACRYGT	1 cut(s)	74
AgeI	ACCGGT	1 cut(s)	479
AgsI	TTSAA	4 cut(s)	418, 773, 1074, 1281
AjnI	CCWGG	1 cut(s)	787
AluBI	AGCT	7 cut(s)	68, 647, 776, 829, 988, 1001, 1250
AluI	AGCT	7 cut(s)	68, 647, 776, 829, 988, 1001, 1250
Alw21I	GWGCWC	1 cut(s)	1201
Alw26I	GTCTC	1 cut(s)	869
AlwI	GGATC	2 cut(s)	125, 1243
AlwNI	CAGNNNCTG	2 cut(s)	668, 1154
Ama87I	CYCGRG	2 cut(s)	996, 1194
AoxI	GGCC	5 cut(s)	228, 319, 485, 834, 1255
ApaI	GGGCCC	1 cut(s)	232
ApeKI	GCWGC	4 cut(s)	599, 1010, 1065, 1247
ApoI	RAATTY	1 cut(s)	1189
ArsI	GACNNNNNNTTYG	4 cut(s)	7, 39, 146, 178
AseI	ATTAAT	1 cut(s)	1119
AsiGI	ACCGGT	1 cut(s)	479
AspLEI	GCGC	2 cut(s)	562, 1093
AspS9I	GGNCC	5 cut(s)	228, 229, 340, 1124, 1256
AsuC2I	CCSGG	2 cut(s)	364, 463
AsuHPI	GGTGA	1 cut(s)	1132
AvaI	CYCGRG	2 cut(s)	996, 1194
AvaII	GGWCC	2 cut(s)	340, 1124
BaeGI	GKGCMC	1 cut(s)	232
BalI	TGGCCA	1 cut(s)	321
BanII	GRGCYC	1 cut(s)	232
Bbv12I	GWGCWC	1 cut(s)	1201
BbvI	GCAGC	4 cut(s)	586, 1022, 1077, 1259
BccI	CCATC	6 cut(s)	254, 397, 447, 1093, 1220, 1284
BceAI	ACGGC	1 cut(s)	1078
BciT130I	CCWGG	1 cut(s)	789
BciVI	GTATCC	2 cut(s)	135, 401
BcnI	CCSGG	2 cut(s)	364, 463
BcoDI	GTCTC	1 cut(s)	869
BfaI	CTAG	3 cut(s)	90, 603, 611
BfuI	GTATCC	2 cut(s)	135, 401
BisI	GCNGC	4 cut(s)	600, 1011, 1066, 1248
BlsI	GCNGC	4 cut(s)	601, 1012, 1067, 1249
Bme1390I	CCNGG	3 cut(s)	364, 463, 789
Bme18I	GGWCC	2 cut(s)	340, 1124
BmeT110I	CYCGRG	2 cut(s)	996, 1194
BmgT120I	GGNCC	5 cut(s)	228, 229, 340, 1124, 1256
BmiI	GGNNCC	4 cut(s)	230, 231, 244, 1126
BmrFI	CCNGG	3 cut(s)	364, 463, 789
BmrI	ACTGGG	1 cut(s)	93
BmsI	GCATC	3 cut(s)	95, 438, 1285
BmuI	ACTGGG	1 cut(s)	93
BpuEI	CTTGAG	1 cut(s)	164
BpuMI	CCSGG	2 cut(s)	364, 463
BsaJI	CCNNGG	1 cut(s)	1162
BsaWI	WCCGGW	1 cut(s)	479
BsaXI	ACNNNNNCTCC	2 cut(s)	936, 966
Bsc4I	CCNNNNNNNGG	6 cut(s)	140, 239, 399, 431, 738, 1185
Bse118I	RCCGGY	2 cut(s)	479, 1013
Bse1I	ACTGG	5 cut(s)	99, 857, 1206, 1237, 1273
Bse3DI	GCAATG	3 cut(s)	151, 205, 1047
BseBI	CCWGG	1 cut(s)	789
BseDI	CCNNGG	1 cut(s)	1162
BseGI	GGATG	5 cut(s)	372, 408, 453, 1295, 1300
BseLI	CCNNNNNNNGG	6 cut(s)	140, 239, 399, 431, 738, 1185
BseMI	GCAATG	3 cut(s)	151, 205, 1047
BseMII	CTCAG	1 cut(s)	864
BseNI	ACTGG	5 cut(s)	99, 857, 1206, 1237, 1273
BseSI	GKGCMC	1 cut(s)	232
BseXI	GCAGC	4 cut(s)	586, 1022, 1077, 1259
BsgI	GTGCAG	1 cut(s)	1266
Bsh1285I	CGRYCG	1 cut(s)	363
BshFI	GGCC	5 cut(s)	230, 321, 487, 836, 1257
BshTI	ACCGGT	1 cut(s)	479
BsiEI	CGRYCG	1 cut(s)	363
BsiHKAI	GWGCWC	1 cut(s)	1201
BsiHKCI	CYCGRG	2 cut(s)	996, 1194
BsiSI	CCGG	5 cut(s)	363, 462, 480, 1014, 1037
BslI	CCNNNNNNNGG	6 cut(s)	140, 239, 399, 431, 738, 1185
BsmAI	GTCTC	1 cut(s)	869
BsmI	GAATGC	1 cut(s)	562
BsnI	GGCC	5 cut(s)	230, 321, 487, 836, 1257
BsoBI	CYCGRG	2 cut(s)	996, 1194
Bsp120I	GGGCCC	1 cut(s)	228
Bsp1286I	GDGCHC	2 cut(s)	232, 1201
Bsp143I	GATC	3 cut(s)	130, 274, 1235
BspACI	CCGC	2 cut(s)	1146, 1298
BspANI	GGCC	5 cut(s)	230, 321, 487, 836, 1257
BspCNI	CTCAG	1 cut(s)	865
BspLI	GGNNCC	4 cut(s)	230, 231, 244, 1126
BspPI	GGATC	2 cut(s)	125, 1243
BsrDI	GCAATG	3 cut(s)	151, 205, 1047
BsrFI	RCCGGY	2 cut(s)	479, 1013
BsrI	ACTGG	5 cut(s)	99, 857, 1206, 1237, 1273
BssAI	RCCGGY	2 cut(s)	479, 1013
BssECI	CCNNGG	1 cut(s)	1162
BssMI	GATC	3 cut(s)	130, 274, 1235
BssT1I	CCWWGG	1 cut(s)	1162
Bst2UI	CCWGG	1 cut(s)	789
Bst4CI	ACNGT	4 cut(s)	7, 713, 1175, 1210
Bst6I	CTCTTC	1 cut(s)	1266
BstAPI	GCANNNNNTGC	1 cut(s)	1154
BstC8I	GCNNGC	4 cut(s)	319, 970, 1015, 1108
BstDEI	CTNAG	2 cut(s)	873, 1181
BstEII	GGTNACC	1 cut(s)	1210
BstF5I	GGATG	5 cut(s)	372, 408, 453, 1295, 1300
BstHHI	GCGC	2 cut(s)	562, 1093
BstKTI	GATC	3 cut(s)	133, 277, 1238
BstMAI	GTCTC	1 cut(s)	869
BstMBI	GATC	3 cut(s)	130, 274, 1235
BstMCI	CGRYCG	1 cut(s)	363
BstMWI	GCNNNNNNNGC	4 cut(s)	287, 1007, 1037, 1154
BstNI	CCWGG	1 cut(s)	789
BstNSI	RCATGY	2 cut(s)	78, 294
BstPI	GGTNACC	1 cut(s)	1210
BstSCI	CCNGG	3 cut(s)	362, 461, 787
BstSLI	GKGCMC	1 cut(s)	232
BstV1I	GCAGC	4 cut(s)	586, 1022, 1077, 1259
BsuI	GTATCC	2 cut(s)	135, 401
BsuRI	GGCC	5 cut(s)	230, 321, 487, 836, 1257
BtsCI	GGATG	5 cut(s)	372, 408, 453, 1295, 1300
BtsIMutI	CAGTG	1 cut(s)	1199
Cac8I	GCNNGC	4 cut(s)	319, 970, 1015, 1108
CaiI	CAGNNNCTG	2 cut(s)	668, 1154
CfoI	GCGC	2 cut(s)	562, 1093
Cfr10I	RCCGGY	2 cut(s)	479, 1013
Cfr13I	GGNCC	5 cut(s)	228, 229, 340, 1124, 1256
Csp6I	GTAC	3 cut(s)	665, 850, 1230
CspAI	ACCGGT	1 cut(s)	479
CspCI	CAANNNNNGTGG	4 cut(s)	813, 848, 1204, 1239
CviAII	CATG	5 cut(s)	75, 83, 291, 703, 1021
CviQI	GTAC	3 cut(s)	665, 850, 1230
DdeI	CTNAG	2 cut(s)	873, 1181
DpnI	GATC	3 cut(s)	132, 276, 1237
DpnII	GATC	3 cut(s)	130, 274, 1235
DrdI	GACNNNNNNGTC	1 cut(s)	367
DseDI	GACNNNNNNGTC	1 cut(s)	367
EaeI	YGGCCR	1 cut(s)	319
Eam1104I	CTCTTC	1 cut(s)	1266
EarI	CTCTTC	1 cut(s)	1266
Eco130I	CCWWGG	1 cut(s)	1162
Eco24I	GRGCYC	1 cut(s)	232
Eco32I	GATATC	2 cut(s)	41, 255
Eco47I	GGWCC	2 cut(s)	340, 1124
Eco57I	CTGAAG	1 cut(s)	474
Eco88I	CYCGRG	2 cut(s)	996, 1194
Eco91I	GGTNACC	1 cut(s)	1210
EcoO109I	RGGNCCY	1 cut(s)	229
EcoO65I	GGTNACC	1 cut(s)	1210
EcoRI	GAATTC	1 cut(s)	1189
EcoRII	CCWGG	1 cut(s)	787
EcoRV	GATATC	2 cut(s)	41, 255
EcoT14I	CCWWGG	1 cut(s)	1162
EcoT22I	ATGCAT	1 cut(s)	88
EcoT38I	GRGCYC	1 cut(s)	232
ErhI	CCWWGG	1 cut(s)	1162
FaeI	CATG	5 cut(s)	78, 86, 294, 706, 1024
FatI	CATG	5 cut(s)	74, 82, 290, 702, 1020
FauNDI	CATATG	1 cut(s)	551
FblI	GTMKAC	1 cut(s)	708
Fnu4HI	GCNGC	4 cut(s)	600, 1011, 1066, 1248
FokI	GGATG	4 cut(s)	379, 415, 460, 1302
FriOI	GRGCYC	1 cut(s)	232
Fsp4HI	GCNGC	4 cut(s)	600, 1011, 1066, 1248
FspAI	RTGCGCAY	1 cut(s)	561
FspBI	CTAG	3 cut(s)	90, 603, 611
FspI	TGCGCA	1 cut(s)	561
GlaI	GCGC	2 cut(s)	561, 1092
GluI	GCNGC	4 cut(s)	600, 1011, 1066, 1248
HaeIII	GGCC	5 cut(s)	230, 321, 487, 836, 1257
HapII	CCGG	5 cut(s)	363, 462, 480, 1014, 1037
HhaI	GCGC	2 cut(s)	562, 1093
Hin1II	CATG	5 cut(s)	78, 86, 294, 706, 1024
Hin6I	GCGC	2 cut(s)	560, 1091
HinP1I	GCGC	2 cut(s)	560, 1091
HincII	GTYRAC	1 cut(s)	709
HindII	GTYRAC	1 cut(s)	709
HindIII	AAGCTT	3 cut(s)	774, 827, 986
HinfI	GANTC	4 cut(s)	22, 155, 699, 960
HpaII	CCGG	5 cut(s)	363, 462, 480, 1014, 1037
HphI	GGTGA	1 cut(s)	1132
Hpy166II	GTNNAC	2 cut(s)	590, 709
Hpy188I	TCNGA	2 cut(s)	347, 764
Hpy188III	TCNNGA	8 cut(s)	90, 128, 181, 250, 355, 611, 732, 1194
Hpy8I	GTNNAC	2 cut(s)	590, 709
Hpy99I	CGWCG	1 cut(s)	713
HpyAV	CCTTC	2 cut(s)	498, 516
HpyCH4III	ACNGT	4 cut(s)	7, 713, 1175, 1210
HpyCH4V	TGCA	8 cut(s)	13, 86, 290, 451, 1031, 1133, 1157, 1247
HpyF10VI	GCNNNNNNNGC	4 cut(s)	287, 1007, 1037, 1154
HpyF3I	CTNAG	2 cut(s)	873, 1181
Hsp92II	CATG	5 cut(s)	78, 86, 294, 706, 1024
HspAI	GCGC	2 cut(s)	560, 1091
KroI	GCCGGC	1 cut(s)	1013
KroNI	GCCGGC	1 cut(s)	1015
Kzo9I	GATC	3 cut(s)	130, 274, 1235
Lsp1109I	GCAGC	4 cut(s)	586, 1022, 1077, 1259
LweI	GCATC	3 cut(s)	95, 438, 1285
MaeI	CTAG	3 cut(s)	90, 603, 611
MaeIII	GTNAC	5 cut(s)	55, 77, 213, 293, 1210
MalI	GATC	3 cut(s)	132, 276, 1237
MboI	GATC	3 cut(s)	130, 274, 1235
MboII	GAAGA	3 cut(s)	689, 808, 1253
MhlI	GDGCHC	2 cut(s)	232, 1201
MlsI	TGGCCA	1 cut(s)	321
MluNI	TGGCCA	1 cut(s)	321
MlyI	GAGTC	2 cut(s)	31, 149
Mox20I	TGGCCA	1 cut(s)	321
Mph1103I	ATGCAT	1 cut(s)	88
MroNI	GCCGGC	1 cut(s)	1013
MscI	TGGCCA	1 cut(s)	321
MseI	TTAA	6 cut(s)	111, 237, 684, 990, 1119, 1306
Msp20I	TGGCCA	1 cut(s)	321
MspI	CCGG	5 cut(s)	363, 462, 480, 1014, 1037
MspR9I	CCNGG	3 cut(s)	364, 463, 789
Mva1269I	GAATGC	1 cut(s)	562
MvaI	CCWGG	1 cut(s)	789
MwoI	GCNNNNNNNGC	4 cut(s)	287, 1007, 1037, 1154
NaeI	GCCGGC	1 cut(s)	1015
NciI	CCSGG	2 cut(s)	364, 463
NdeI	CATATG	1 cut(s)	551
NdeII	GATC	3 cut(s)	130, 274, 1235
NgoMIV	GCCGGC	1 cut(s)	1013
NlaIII	CATG	5 cut(s)	78, 86, 294, 706, 1024
NlaIV	GGNNCC	4 cut(s)	230, 231, 244, 1126
NsbI	TGCGCA	1 cut(s)	561
NsiI	ATGCAT	1 cut(s)	88
NspI	RCATGY	2 cut(s)	78, 294
PaeR7I	CTCGAG	1 cut(s)	1194
PciI	ACATGT	1 cut(s)	74
PctI	GAATGC	1 cut(s)	562
PdiI	GCCGGC	1 cut(s)	1015
PfeI	GAWTC	2 cut(s)	699, 960
PinAI	ACCGGT	1 cut(s)	479
PkrI	GCNGC	4 cut(s)	601, 1012, 1067, 1249
PleI	GAGTC	2 cut(s)	30, 149
PpsI	GAGTC	2 cut(s)	30, 149
PscI	ACATGT	1 cut(s)	74
PshBI	ATTAAT	1 cut(s)	1119
Psp6I	CCWGG	1 cut(s)	787
PspEI	GGTNACC	1 cut(s)	1210
PspGI	CCWGG	1 cut(s)	787
PspN4I	GGNNCC	4 cut(s)	230, 231, 244, 1126
PspOMI	GGGCCC	1 cut(s)	228
PspPI	GGNCC	5 cut(s)	228, 229, 340, 1124, 1256
PstNI	CAGNNNCTG	2 cut(s)	668, 1154
RsaI	GTAC	3 cut(s)	666, 851, 1231
RsaNI	GTAC	3 cut(s)	665, 850, 1230
SalI	GTCGAC	1 cut(s)	707
SaqAI	TTAA	6 cut(s)	111, 237, 684, 990, 1119, 1306
SatI	GCNGC	4 cut(s)	600, 1011, 1066, 1248
Sau3AI	GATC	3 cut(s)	130, 274, 1235
Sau96I	GGNCC	5 cut(s)	228, 229, 340, 1124, 1256
SchI	GAGTC	2 cut(s)	31, 149
ScrFI	CCNGG	3 cut(s)	364, 463, 789
SduI	GDGCHC	2 cut(s)	232, 1201
SfaNI	GCATC	3 cut(s)	95, 438, 1285
Sfr274I	CTCGAG	1 cut(s)	1194
SinI	GGWCC	2 cut(s)	340, 1124
SlaI	CTCGAG	1 cut(s)	1194
SmlI	CTYRAG	2 cut(s)	179, 1194
SmoI	CTYRAG	2 cut(s)	179, 1194
SsiI	CCGC	2 cut(s)	1146, 1298
SspI	AATATT	2 cut(s)	421, 1087
SspMI	CTAG	3 cut(s)	90, 603, 611
StyD4I	CCNGG	3 cut(s)	362, 461, 787
StyI	CCWWGG	1 cut(s)	1162
TaaI	ACNGT	4 cut(s)	7, 713, 1175, 1210
TaqI	TCGA	5 cut(s)	257, 708, 936, 1054, 1195
TatI	WGTACW	1 cut(s)	849
TfiI	GAWTC	2 cut(s)	699, 960
Tru1I	TTAA	6 cut(s)	111, 237, 684, 990, 1119, 1306
Tru9I	TTAA	6 cut(s)	111, 237, 684, 990, 1119, 1306
TscAI	CASTG	1 cut(s)	1206
TseI	GCWGC	4 cut(s)	599, 1010, 1065, 1247
TspDTI	ATGAA	5 cut(s)	52, 690, 691, 704, 1009
TspRI	CASTG	1 cut(s)	1206
VpaK11BI	GGWCC	2 cut(s)	340, 1124
VspI	ATTAAT	1 cut(s)	1119
XapI	RAATTY	1 cut(s)	1189
XbaI	TCTAGA	2 cut(s)	89, 610
XceI	RCATGY	2 cut(s)	78, 294
XcmI	CCANNNNNNNNNTGG	1 cut(s)	143
XhoI	CTCGAG	1 cut(s)	1194
XmiI	GTMKAC	1 cut(s)	708
XspI	CTAG	3 cut(s)	90, 603, 611
Zsp2I	ATGCAT	1 cut(s)	88

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.