FvH4_5g20760

Novel plant snare

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013342
Physical Location & Seq
Forward (+)
12330346 .. 12336735
6390 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20760.t1

Sequence Viewer

Length: 624 bp
ATGGATCCGTTATCGGCAATCAGCGAGGAGCTGGCGGAGATCGACGGCCAAATCGCCGACGTCTTCCGTGCGTTATCAAATGGGTTTCAGAAGCTGGAGAAGATTAAGGACTCGAATAGGCAGAGCCGGCAGCTGGAGGATCTCACTGATAAGATGCGCGACTGTAAGAGACTTATTAAAGAGTTTGACAAGGAAGTCAAGAATATGGAGAGAAGAATTGATCCAGACTCAAGTAGAATGCTGAGTGAGAAAAAGCAGTCAATGATCAAAGAGTTGAACTCATACGTGGCTTTGAAGAAACAATATGCAACAAATCTTGAAAACAAGAAAGCCGATCTCTTTGATGTGCCAGCTAATGAATATGCTGAACAAAATGTGTTGCTAGCCTCAGGTATGACAAATCAGCAACTTATTGATAATGGACATCAAATGATGGATGAGACTGATCAAGCCATTGATAGAGCACAAAAGGTTGTTCATGAGACTATCAACGTTGGAACAGAGACAGCAGCAACTCTCAAGGCTCAAACTGAACAAATGAGTAGGATTGTTAACGAGCTGGACTCTATCCATTTCTCAATCAAGAAGGCTACTAAGCTGGTGAAGGAAATTGGTTGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec20

Protein Analysis

208

Amino Acids

23.6

Weight (kDa)

5.4

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35190
fragaria_vesca FvH4_5g20760 FvH4_5g20760
malus_domestica MD04G1053700.v1.1 MD11G1036500.v1.1
prunus_persica Prupe.5G052300_v2.0.a1 Prupe.5G052300_v2.0.a1
pyrus_communis pycom04g04680 pycom11g03010
rosa_chinensis RchiOBHm_Chr7g0206541
rosa_laevigata RLG00000003304
rosa_multiflora Rmu_sc0005065.1_g000056
rosa_roxburghii Rroxscaffold_3G00251420
rosa_rugosa Rorug07G0098300 Rorug07G0098400
rosa_samantha Rh7AG229800 Rh7CG244700
rosa_wichuraiana Rw7G019810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
AatII GACGTC 1 cut(s) 63
AccII CGCG 1 cut(s) 159
AciI CCGC 1 cut(s) 35
AclI AACGTT 1 cut(s) 492
AclWI GGATC 3 cut(s) 12, 147, 215
AcoI YGGCCR 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 133
AgsI TTSAA 3 cut(s) 277, 295, 320
AluBI AGCT 6 cut(s) 31, 94, 133, 353, 559, 598
AluI AGCT 6 cut(s) 31, 94, 133, 353, 559, 598
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 4 cut(s) 163, 434, 476, 497
AlwI GGATC 3 cut(s) 12, 147, 215
AlwNI CAGNNNCTG 1 cut(s) 94
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 2 cut(s) 130, 509
AspLEI GCGC 1 cut(s) 159
AsuHPI GGTGA 1 cut(s) 613
AsuNHI GCTAGC 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 388
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 55
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 2 cut(s) 142, 521
BccI CCATC 1 cut(s) 427
BceAI ACGGC 1 cut(s) 61
BclI TGATCA 2 cut(s) 264, 445
BcoDI GTCTC 4 cut(s) 163, 434, 476, 497
BfaI CTAG 1 cut(s) 383
BisI GCNGC 2 cut(s) 131, 510
BlsI GCNGC 2 cut(s) 132, 511
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 1 cut(s) 144
BmtI GCTAGC 1 cut(s) 386
BpiI GAAGAC 1 cut(s) 55
BplI GAGNNNNNCTC 4 cut(s) 263, 295, 548, 580
BpmI CTGGAG 2 cut(s) 116, 155
BpuEI CTTGAG 2 cut(s) 214, 503
BsaAI YACGTR 1 cut(s) 286
BsaHI GRCGYC 1 cut(s) 60
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse118I RCCGGY 1 cut(s) 126
Bse21I CCTNAGG 1 cut(s) 388
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 2 cut(s) 233, 402
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 2 cut(s) 142, 521
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 4 cut(s) 163, 434, 476, 497
BsmI GAATGC 1 cut(s) 243
BsnI GGCC 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 7 cut(s) 4, 39, 139, 220, 264, 334, 445
BspACI CCGC 1 cut(s) 35
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 2 cut(s) 234, 401
BspFNI CGCG 1 cut(s) 159
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 6
BspOI GCTAGC 1 cut(s) 386
BspPI GGATC 3 cut(s) 12, 147, 215
BsrFI RCCGGY 1 cut(s) 126
BssAI RCCGGY 1 cut(s) 126
BssMI GATC 7 cut(s) 4, 39, 139, 220, 264, 334, 445
BssNI GRCGYC 1 cut(s) 60
Bst4CI ACNGT 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 60
BstBAI YACGTR 1 cut(s) 286
BstC8I GCNNGC 4 cut(s) 33, 128, 351, 384
BstDEI CTNAG 3 cut(s) 242, 388, 594
BstF5I GGATG 1 cut(s) 442
BstFNI CGCG 1 cut(s) 159
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 7 cut(s) 7, 42, 142, 223, 267, 337, 448
BstMAI GTCTC 4 cut(s) 163, 434, 476, 497
BstMBI GATC 7 cut(s) 4, 39, 139, 220, 264, 334, 445
BstMWI GCNNNNNNNGC 1 cut(s) 127
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 142, 521
BstV2I GAAGAC 1 cut(s) 55
BstX2I RGATCY 2 cut(s) 4, 139
BstYI RGATCY 2 cut(s) 4, 139
Bsu36I CCTNAGG 1 cut(s) 388
BsuRI GGCC 1 cut(s) 48
BtsCI GGATG 1 cut(s) 442
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 4 cut(s) 33, 128, 351, 384
CaiI CAGNNNCTG 1 cut(s) 94
CciI TCATGA 1 cut(s) 478
CfoI GCGC 1 cut(s) 159
Cfr10I RCCGGY 1 cut(s) 126
CviAII CATG 1 cut(s) 479
DdeI CTNAG 3 cut(s) 242, 388, 594
DpnI GATC 7 cut(s) 6, 41, 141, 222, 266, 336, 447
DpnII GATC 7 cut(s) 4, 39, 139, 220, 264, 334, 445
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
EaeI YGGCCR 1 cut(s) 46
EciI GGCGGA 1 cut(s) 50
Eco81I CCTNAGG 1 cut(s) 388
FaeI CATG 1 cut(s) 482
FaiI YATR 6 cut(s) 206, 283, 306, 363, 395, 480
FatI CATG 1 cut(s) 478
FbaI TGATCA 2 cut(s) 264, 445
Fnu4HI GCNGC 2 cut(s) 131, 510
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 2 cut(s) 131, 510
FspBI CTAG 1 cut(s) 383
GlaI GCGC 1 cut(s) 158
GluI GCNGC 2 cut(s) 131, 510
GsuI CTGGAG 2 cut(s) 116, 155
HaeIII GGCC 1 cut(s) 48
HapII CCGG 1 cut(s) 127
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 1 cut(s) 482
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 3 cut(s) 110, 227, 563
HpaI GTTAAC 1 cut(s) 553
HpaII CCGG 1 cut(s) 127
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 5 cut(s) 199, 224, 317, 479, 583
Hpy8I GTNNAC 1 cut(s) 553
Hpy99I CGWCG 2 cut(s) 47, 62
HpyAV CCTTC 2 cut(s) 580, 598
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4IV ACGT 3 cut(s) 60, 285, 492
HpyCH4V TGCA 2 cut(s) 308, 618
HpyF10VI GCNNNNNNNGC 1 cut(s) 127
HpyF3I CTNAG 3 cut(s) 242, 388, 594
HpySE526I ACGT 3 cut(s) 60, 285, 492
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 1 cut(s) 482
HspAI GCGC 1 cut(s) 157
KroI GCCGGC 1 cut(s) 126
KroNI GCCGGC 1 cut(s) 128
Ksp22I TGATCA 2 cut(s) 264, 445
KspAI GTTAAC 1 cut(s) 553
Kzo9I GATC 7 cut(s) 4, 39, 139, 220, 264, 334, 445
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 9 cut(s) 17, 80, 119, 140, 237, 363, 375, 545, 584
Lsp1109I GCAGC 2 cut(s) 142, 521
LweI GCATC 1 cut(s) 144
MaeI CTAG 1 cut(s) 383
MaeII ACGT 3 cut(s) 60, 285, 492
MalI GATC 7 cut(s) 6, 41, 141, 222, 266, 336, 447
MboI GATC 7 cut(s) 4, 39, 139, 220, 264, 334, 445
MboII GAAGA 4 cut(s) 55, 112, 225, 307
MflI RGATCY 2 cut(s) 4, 139
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 2 cut(s) 216, 609
MlyI GAGTC 3 cut(s) 104, 221, 557
MmeI TCCRAC 1 cut(s) 475
MnlI CCTC 3 cut(s) 19, 130, 397
MroNI GCCGGC 1 cut(s) 126
MseI TTAA 3 cut(s) 105, 177, 552
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 1 cut(s) 127
Mva1269I GAATGC 1 cut(s) 243
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 127
NaeI GCCGGC 1 cut(s) 128
NdeII GATC 7 cut(s) 4, 39, 139, 220, 264, 334, 445
NgoMIV GCCGGC 1 cut(s) 126
NheI GCTAGC 1 cut(s) 382
NlaIII CATG 1 cut(s) 482
NlaIV GGNNCC 1 cut(s) 6
PagI TCATGA 1 cut(s) 478
PctI GAATGC 1 cut(s) 243
PdiI GCCGGC 1 cut(s) 128
PkrI GCNGC 2 cut(s) 132, 511
PleI GAGTC 3 cut(s) 104, 221, 557
PpsI GAGTC 3 cut(s) 104, 221, 557
Ppu21I YACGTR 1 cut(s) 286
Psp1406I AACGTT 1 cut(s) 492
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 94
PsuI RGATCY 2 cut(s) 4, 139
PvuII CAGCTG 1 cut(s) 133
SaqAI TTAA 3 cut(s) 105, 177, 552
SatI GCNGC 2 cut(s) 131, 510
Sau3AI GATC 7 cut(s) 4, 39, 139, 220, 264, 334, 445
SchI GAGTC 3 cut(s) 104, 221, 557
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 1 cut(s) 144
SmlI CTYRAG 2 cut(s) 229, 518
SmoI CTYRAG 2 cut(s) 229, 518
Sse9I AATT 2 cut(s) 216, 609
SsiI CCGC 1 cut(s) 35
SspMI CTAG 1 cut(s) 383
TaaI ACNGT 1 cut(s) 164
TaiI ACGT 3 cut(s) 63, 288, 495
TaqI TCGA 2 cut(s) 42, 113
TasI AATT 2 cut(s) 216, 609
Tru1I TTAA 3 cut(s) 105, 177, 552
Tru9I TTAA 3 cut(s) 105, 177, 552
TscAI CASTG 1 cut(s) 151
TseI GCWGC 2 cut(s) 130, 509
TspDTI ATGAA 2 cut(s) 372, 467
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 1 cut(s) 151
XspI CTAG 1 cut(s) 383
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.