MD11G1036500.v1.1

Novel plant snare

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013342
Physical Location & Seq
Forward (+)
3156120 .. 3161142
5023 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1036500.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGATCCGTTATCTTCAATCAGCGAAGAGCTGGCGGAGATCGACGGACAAATCACCGACATTTTTCGCGCATTATCAAATGGGTTTCAGAAGCTGGAGAAGATTAAGGACTCGAATAGGCGGAGTAGGCAGCTGGAAGAGCTCACTGAAAAGATGCGAGACTGTAAGAGTTGGCTTATCAAAGAATTTGACAGAGAAGTGAAGAATATGGAGAGAATAAATGATCCAAATACCAGTAGAATGCTGAATGAGAAAAAGCAGTCGATGATTAAAGAGTTGAATTCTTATGTGGCTCTGAAAAAACAATATGCATCAAACCTTGACAACAAGAAAATTGATCTCTTTGATGGACCGGCCGAAGACTTGGGGGAACAGAATGTCTTGCTAGCCTCGGCTATGACGAATCAACAACTTATTGATAATGGAAACCAAATGATGGATGAGACAGATCAAGCCATTGAGAGATCAAAGAAGGTTGTTCATGAAACCATCAATGTTGGAACAGAGACAGCACATGCTCTCAAGGCTCAAACGGAACAAATGAGCAGGATTGTCAACGAGCTGGACTCTATACATTTCTCAATCAAGAAAGCTTCTAAGCTGGTCAAGGAAATTGGTAGGCAGGTTGCAACTGATAGGTGTATTATGGGATTACTCTTCTTGATTGTTGTAGGCGTCATAGCCATCATTATTGTGAAGCTGGTTCATCCAAACAACAAGGACATTCGAGATATTCCTGGATTAGCCCCTCCCGCAATGACTCGAAAGCTACTGTGGAATTCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec20

Protein Analysis

263

Amino Acids

29.84

Weight (kDa)

6.47

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-SNARE_C PF12352 149 - 207 9.7e-06 Snare region anchored in the vesicle membrane C-terminus
Sec20 PF03908 151 - 226 4.8e-07 Sec20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35190
fragaria_vesca FvH4_5g20760 FvH4_5g20760
malus_domestica MD04G1053700.v1.1 MD11G1036500.v1.1
prunus_persica Prupe.5G052300_v2.0.a1 Prupe.5G052300_v2.0.a1
pyrus_communis pycom04g04680 pycom11g03010
rosa_chinensis RchiOBHm_Chr7g0206541
rosa_laevigata RLG00000003304
rosa_multiflora Rmu_sc0005065.1_g000056
rosa_roxburghii Rroxscaffold_3G00251420
rosa_rugosa Rorug07G0098300 Rorug07G0098400
rosa_samantha Rh7AG229800 Rh7CG244700
rosa_wichuraiana Rw7G019810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 613
AccB7I CCANNNNNTGG 1 cut(s) 436
AccII CGCG 1 cut(s) 69
AciI CCGC 3 cut(s) 35, 121, 753
AclWI GGATC 2 cut(s) 12, 218
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 3 cut(s) 185, 280, 778
AcyI GRCGYC 1 cut(s) 675
AfiI CCNNNNNNNGG 1 cut(s) 436
AgsI TTSAA 2 cut(s) 18, 280
AjnI CCWGG 1 cut(s) 736
AluBI AGCT 9 cut(s) 31, 94, 133, 142, 562, 593, 601, 700, 769
AluI AGCT 9 cut(s) 31, 94, 133, 142, 562, 593, 601, 700, 769
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 3 cut(s) 153, 437, 500
AlwI GGATC 2 cut(s) 12, 218
AlwNI CAGNNNCTG 1 cut(s) 94
AoxI GGCC 1 cut(s) 354
ApeKI GCWGC 1 cut(s) 130
ApoI RAATTY 3 cut(s) 185, 280, 778
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 350
AsuHPI GGTGA 1 cut(s) 46
AsuNHI GCTAGC 1 cut(s) 385
AvaII GGWCC 1 cut(s) 350
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 144
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 142
BccI CCATC 4 cut(s) 341, 430, 497, 692
BciT130I CCWGG 1 cut(s) 738
BcoDI GTCTC 3 cut(s) 153, 437, 500
BfaI CTAG 1 cut(s) 386
BfuAI ACCTGC 1 cut(s) 613
BisI GCNGC 1 cut(s) 131
BlsI GCNGC 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 738
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 350
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 738
BmsI GCATC 2 cut(s) 144, 320
BmtI GCTAGC 1 cut(s) 389
BpiI GAAGAC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 551, 583
BpmI CTGGAG 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 506
BsaHI GRCGYC 1 cut(s) 675
BsaJI CCNNGG 1 cut(s) 390
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse118I RCCGGY 1 cut(s) 352
Bse1I ACTGG 1 cut(s) 234
Bse3DI GCAATG 1 cut(s) 762
BseBI CCWGG 1 cut(s) 738
BseDI CCNNGG 1 cut(s) 390
BseGI GGATG 2 cut(s) 445, 706
BseLI CCNNNNNNNGG 1 cut(s) 436
BseMI GCAATG 1 cut(s) 762
BseNI ACTGG 1 cut(s) 234
BseX3I CGGCCG 1 cut(s) 354
BseXI GCAGC 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 1 cut(s) 353
BslI CCNNNNNNNGG 1 cut(s) 436
BsmAI GTCTC 3 cut(s) 153, 437, 500
BsmI GAATGC 1 cut(s) 246
BsnI GGCC 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 6 cut(s) 4, 39, 223, 337, 448, 464
BspACI CCGC 3 cut(s) 35, 121, 753
BspANI GGCC 1 cut(s) 356
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 613
BspOI GCTAGC 1 cut(s) 389
BspPI GGATC 2 cut(s) 12, 218
BspQI GCTCTTC 2 cut(s) 21, 132
BsrDI GCAATG 1 cut(s) 762
BsrFI RCCGGY 1 cut(s) 352
BsrI ACTGG 1 cut(s) 234
BssAI RCCGGY 1 cut(s) 352
BssECI CCNNGG 1 cut(s) 390
BssMI GATC 6 cut(s) 4, 39, 223, 337, 448, 464
BssNI GRCGYC 1 cut(s) 675
Bst2UI CCWGG 1 cut(s) 738
Bst4CI ACNGT 2 cut(s) 164, 774
Bst6I CTCTTC 3 cut(s) 21, 132, 662
BstACI GRCGYC 1 cut(s) 675
BstC8I GCNNGC 2 cut(s) 33, 387
BstDEI CTNAG 1 cut(s) 597
BstF5I GGATG 2 cut(s) 445, 706
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 6 cut(s) 7, 42, 226, 340, 451, 467
BstMAI GTCTC 3 cut(s) 153, 437, 500
BstMBI GATC 6 cut(s) 4, 39, 223, 337, 448, 464
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 4 cut(s) 127, 139, 524, 752
BstNI CCWGG 1 cut(s) 738
BstNSI RCATGY 1 cut(s) 518
BstSCI CCNGG 1 cut(s) 736
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZI CGGCCG 1 cut(s) 354
BsuRI GGCC 1 cut(s) 356
BtsCI GGATG 2 cut(s) 445, 706
BtsIMutI CAGTG 1 cut(s) 144
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 2 cut(s) 33, 387
CaiI CAGNNNCTG 1 cut(s) 94
CciI TCATGA 1 cut(s) 481
CfoI GCGC 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 352
Cfr13I GGNCC 1 cut(s) 350
CseI GACGC 1 cut(s) 664
CviAII CATG 2 cut(s) 482, 515
DdeI CTNAG 1 cut(s) 597
DpnI GATC 6 cut(s) 6, 41, 225, 339, 450, 466
DpnII GATC 6 cut(s) 4, 39, 223, 337, 448, 464
EaeI YGGCCR 1 cut(s) 354
EagI CGGCCG 1 cut(s) 354
Eam1104I CTCTTC 3 cut(s) 21, 132, 662
EarI CTCTTC 3 cut(s) 21, 132, 662
EciI GGCGGA 2 cut(s) 50, 136
Ecl136II GAGCTC 1 cut(s) 142
EclXI CGGCCG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 350
Eco52I CGGCCG 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 142
EcoRI GAATTC 2 cut(s) 280, 778
EcoRII CCWGG 1 cut(s) 736
EcoT22I ATGCAT 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 144
FaeI CATG 2 cut(s) 485, 518
FaiI YATR 9 cut(s) 209, 288, 309, 398, 483, 516, 572, 647, 680
FatI CATG 2 cut(s) 481, 514
FauI CCCGC 1 cut(s) 760
Fnu4HI GCNGC 1 cut(s) 131
FokI GGATG 2 cut(s) 452, 693
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 1 cut(s) 131
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 70
GluI GCNGC 1 cut(s) 131
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 1 cut(s) 356
HapII CCGG 1 cut(s) 353
HgaI GACGC 1 cut(s) 664
HhaI GCGC 1 cut(s) 71
Hin1I GRCGYC 1 cut(s) 675
Hin1II CATG 2 cut(s) 485, 518
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 4 cut(s) 110, 403, 566, 760
HpaII CCGG 1 cut(s) 353
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 2 cut(s) 90, 297
Hpy188III TCNNGA 4 cut(s) 482, 586, 661, 728
Hpy8I GTNNAC 1 cut(s) 556
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 2 cut(s) 164, 774
HpyCH4V TGCA 2 cut(s) 311, 629
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 139, 524, 752
HpyF3I CTNAG 1 cut(s) 597
Hsp92I GRCGYC 1 cut(s) 675
Hsp92II CATG 2 cut(s) 485, 518
HspAI GCGC 1 cut(s) 69
Kzo9I GATC 6 cut(s) 4, 39, 223, 337, 448, 464
LguI GCTCTTC 2 cut(s) 21, 132
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 2 cut(s) 144, 320
MaeI CTAG 1 cut(s) 386
MalI GATC 6 cut(s) 6, 41, 225, 339, 450, 466
MboI GATC 6 cut(s) 4, 39, 223, 337, 448, 464
MboII GAAGA 7 cut(s) 6, 38, 112, 149, 214, 371, 649
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 5 cut(s) 185, 280, 333, 612, 778
MlyI GAGTC 3 cut(s) 104, 560, 754
MmeI TCCRAC 1 cut(s) 478
MnlI CCTC 2 cut(s) 400, 759
Mph1103I ATGCAT 1 cut(s) 313
MseI TTAA 2 cut(s) 105, 270
MslI CAYNNNNRTG 1 cut(s) 692
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 1 cut(s) 738
Mva1269I GAATGC 1 cut(s) 246
MvaI CCWGG 1 cut(s) 738
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 4 cut(s) 127, 139, 524, 752
NdeII GATC 6 cut(s) 4, 39, 223, 337, 448, 464
NheI GCTAGC 1 cut(s) 385
NlaIII CATG 2 cut(s) 485, 518
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 371
NsiI ATGCAT 1 cut(s) 313
NspI RCATGY 1 cut(s) 518
PagI TCATGA 1 cut(s) 481
PciSI GCTCTTC 2 cut(s) 21, 132
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PctI GAATGC 1 cut(s) 246
PfeI GAWTC 1 cut(s) 403
PflMI CCANNNNNTGG 1 cut(s) 436
PfoI TCCNGGA 1 cut(s) 736
PkrI GCNGC 1 cut(s) 132
PleI GAGTC 3 cut(s) 104, 560, 754
PpsI GAGTC 3 cut(s) 104, 560, 754
Psp124BI GAGCTC 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 736
PspGI CCWGG 1 cut(s) 736
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 350
PstNI CAGNNNCTG 1 cut(s) 94
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 692
SacI GAGCTC 1 cut(s) 144
SapI GCTCTTC 2 cut(s) 21, 132
SaqAI TTAA 2 cut(s) 105, 270
SatI GCNGC 1 cut(s) 131
Sau3AI GATC 6 cut(s) 4, 39, 223, 337, 448, 464
Sau96I GGNCC 1 cut(s) 350
SchI GAGTC 3 cut(s) 104, 560, 754
ScrFI CCNGG 1 cut(s) 738
SduI GDGCHC 1 cut(s) 144
SfaNI GCATC 2 cut(s) 144, 320
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 5 cut(s) 185, 280, 333, 612, 778
SsiI CCGC 3 cut(s) 35, 121, 753
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 1 cut(s) 736
TaaI ACNGT 2 cut(s) 164, 774
TaqI TCGA 5 cut(s) 42, 113, 263, 727, 763
TasI AATT 5 cut(s) 185, 280, 333, 612, 778
TfiI GAWTC 1 cut(s) 403
Tru1I TTAA 2 cut(s) 105, 270
Tru9I TTAA 2 cut(s) 105, 270
TscAI CASTG 1 cut(s) 151
TseI GCWGC 1 cut(s) 130
TspDTI ATGAA 3 cut(s) 470, 498, 695
TspGWI ACGGA 2 cut(s) 60, 548
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 436
VpaK11BI GGWCC 1 cut(s) 350
XapI RAATTY 3 cut(s) 185, 280, 778
XceI RCATGY 1 cut(s) 518
XspI CTAG 1 cut(s) 386
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.