Rh7AG229800

Novel plant snare

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0013342
Physical Location & Seq
Forward (+)
21761676 .. 21768836
7161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG229800.1

Sequence Viewer

Length: 732 bp
ATGGATCCGTTATCTGCAATCAGCGAGGAGCTGGCGGAGATCGACGGCCAAATCGCCGACGTCTTCCGTGCGTTATCGAATGGGTTTCAGAAGGTGGAGAAGATCAAGGACTCGAATAGGCAGAGCCGGCAGCTGGAAGATCTCACTGATAAGATGCGGGATTGTAAGAGACTTATCAAAGAGTTTGACAAGGAAGTAAAGAATATGGAGAGACGAATTGATCCAGACACAAGTAGAATCCTGAGTGAGAAAAAGCAATCAATGATCAAAGAGTTGAACTCATATGTGGCTTTGAAAAAACAATATGCAACAAATCTTGAAAACAAGAAAGTGGATCTCTTTGATGCACCAGCTAACGAATACGGGGAACAAAATGTGTTGCTTGCCTCGGGTATGACAAATCAGCAACTTATTGATAATGGACACCAAATGATGGATGAGACTGATCAAGCCATCGATAGAGCACAAAAGGTTGTTCATGAGACTATCAATGTTGGAACAGAGACAGCAGCAACTCTCAAGGCTCAAACTGAACAAATGAGTAGGATTGTTAACGAGCTGGACTCTATCCATTTCTCAATCAAGAAGGCTACCAAGCTGGTGAAGGAAATTGGTAGGCAGGTTGCAACTGATAGGTGTATTATGGGATTTCTTGTCCTTATTGTCATTGGAGTCATAGCCATCATCATTGTGAAGGTATGTAATGCATACAACAGATTTCTTTTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec20

Protein Analysis

243

Amino Acids

27.61

Weight (kDa)

6.03

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec20 PF03908 151 - 223 1.8e-06 Sec20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35190
fragaria_vesca FvH4_5g20760 FvH4_5g20760
malus_domestica MD04G1053700.v1.1 MD11G1036500.v1.1
prunus_persica Prupe.5G052300_v2.0.a1 Prupe.5G052300_v2.0.a1
pyrus_communis pycom04g04680 pycom11g03010
rosa_chinensis RchiOBHm_Chr7g0206541
rosa_laevigata RLG00000003304
rosa_multiflora Rmu_sc0005065.1_g000056
rosa_roxburghii Rroxscaffold_3G00251420
rosa_rugosa Rorug07G0098300 Rorug07G0098400
rosa_samantha Rh7AG229800 Rh7CG244700
rosa_wichuraiana Rw7G019810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
Acc36I ACCTGC 1 cut(s) 610
AccB7I CCANNNNNTGG 1 cut(s) 433
AciI CCGC 2 cut(s) 35, 157
AclWI GGATC 3 cut(s) 12, 215, 342
AcoI YGGCCR 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 60
AfiI CCNNNNNNNGG 2 cut(s) 133, 433
AgsI TTSAA 3 cut(s) 277, 295, 320
AluBI AGCT 5 cut(s) 31, 133, 353, 559, 598
AluI AGCT 5 cut(s) 31, 133, 353, 559, 598
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 5 cut(s) 163, 205, 434, 476, 497
AlwI GGATC 3 cut(s) 12, 215, 342
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 2 cut(s) 130, 509
AsuHPI GGTGA 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 388
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 55
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 2 cut(s) 142, 521
BccI CCATC 3 cut(s) 427, 461, 689
BceAI ACGGC 1 cut(s) 61
BclI TGATCA 2 cut(s) 264, 445
BcoDI GTCTC 5 cut(s) 163, 205, 434, 476, 497
BfuAI ACCTGC 1 cut(s) 610
BglII AGATCT 1 cut(s) 139
BisI GCNGC 2 cut(s) 131, 510
BlsI GCNGC 2 cut(s) 132, 511
BmeT110I CYCGRG 1 cut(s) 388
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 2 cut(s) 144, 334
BpiI GAAGAC 1 cut(s) 55
BplI GAGNNNNNCTC 4 cut(s) 263, 295, 548, 580
BpuEI CTTGAG 1 cut(s) 503
Bsa29I ATCGAT 1 cut(s) 456
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 387
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 433
Bse118I RCCGGY 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 2 cut(s) 133, 433
BseMII CTCAG 1 cut(s) 233
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 2 cut(s) 142, 521
BshFI GGCC 1 cut(s) 48
BshVI ATCGAT 1 cut(s) 456
BsiHKAI GWGCWC 1 cut(s) 466
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 133, 433
BsmAI GTCTC 5 cut(s) 163, 205, 434, 476, 497
BsmBI CGTCTC 1 cut(s) 205
BsnI GGCC 1 cut(s) 48
BsoBI CYCGRG 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 8 cut(s) 4, 39, 102, 139, 220, 264, 334, 445
BspACI CCGC 2 cut(s) 35, 157
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 1 cut(s) 234
BspDI ATCGAT 1 cut(s) 456
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 610
BspPI GGATC 3 cut(s) 12, 215, 342
BsrFI RCCGGY 1 cut(s) 126
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 8 cut(s) 4, 39, 102, 139, 220, 264, 334, 445
BssNI GRCGYC 1 cut(s) 60
BstACI GRCGYC 1 cut(s) 60
BstC8I GCNNGC 3 cut(s) 33, 128, 384
BstDEI CTNAG 1 cut(s) 242
BstF5I GGATG 1 cut(s) 442
BstKTI GATC 8 cut(s) 7, 42, 105, 142, 223, 267, 337, 448
BstMAI GTCTC 5 cut(s) 163, 205, 434, 476, 497
BstMBI GATC 8 cut(s) 4, 39, 102, 139, 220, 264, 334, 445
BstMWI GCNNNNNNNGC 1 cut(s) 127
BstV1I GCAGC 2 cut(s) 142, 521
BstV2I GAAGAC 1 cut(s) 55
BstX2I RGATCY 3 cut(s) 4, 139, 334
BstYI RGATCY 3 cut(s) 4, 139, 334
Bsu15I ATCGAT 1 cut(s) 456
BsuRI GGCC 1 cut(s) 48
BsuTUI ATCGAT 1 cut(s) 456
BtsCI GGATG 1 cut(s) 442
BtsIMutI CAGTG 1 cut(s) 144
BveI ACCTGC 1 cut(s) 610
Cac8I GCNNGC 3 cut(s) 33, 128, 384
CciI TCATGA 1 cut(s) 478
Cfr10I RCCGGY 1 cut(s) 126
ClaI ATCGAT 1 cut(s) 456
CviAII CATG 1 cut(s) 479
DdeI CTNAG 1 cut(s) 242
DpnI GATC 8 cut(s) 6, 41, 104, 141, 222, 266, 336, 447
DpnII GATC 8 cut(s) 4, 39, 102, 139, 220, 264, 334, 445
EaeI YGGCCR 1 cut(s) 46
EciI GGCGGA 1 cut(s) 50
Eco88I CYCGRG 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 709
Esp3I CGTCTC 1 cut(s) 205
FaeI CATG 1 cut(s) 482
FatI CATG 1 cut(s) 478
FauI CCCGC 1 cut(s) 150
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 2 cut(s) 264, 445
Fnu4HI GCNGC 2 cut(s) 131, 510
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 2 cut(s) 131, 510
GluI GCNGC 2 cut(s) 131, 510
HaeIII GGCC 1 cut(s) 48
HapII CCGG 1 cut(s) 127
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 1 cut(s) 482
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 4 cut(s) 110, 237, 563, 672
HpaI GTTAAC 1 cut(s) 553
HpaII CCGG 1 cut(s) 127
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 5 cut(s) 224, 241, 317, 479, 583
Hpy8I GTNNAC 1 cut(s) 553
Hpy99I CGWCG 2 cut(s) 47, 62
HpyAV CCTTC 4 cut(s) 85, 580, 598, 688
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 5 cut(s) 17, 308, 347, 626, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 127
HpyF3I CTNAG 1 cut(s) 242
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 1 cut(s) 482
KroI GCCGGC 1 cut(s) 126
KroNI GCCGGC 1 cut(s) 128
Ksp22I TGATCA 2 cut(s) 264, 445
KspAI GTTAAC 1 cut(s) 553
Kzo9I GATC 8 cut(s) 4, 39, 102, 139, 220, 264, 334, 445
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 9 cut(s) 17, 119, 140, 237, 254, 363, 545, 584, 605
Lsp1109I GCAGC 2 cut(s) 142, 521
LweI GCATC 2 cut(s) 144, 334
MaeII ACGT 1 cut(s) 60
MalI GATC 8 cut(s) 6, 41, 104, 141, 222, 266, 336, 447
MboI GATC 8 cut(s) 4, 39, 102, 139, 220, 264, 334, 445
MboII GAAGA 3 cut(s) 55, 112, 149
MflI RGATCY 3 cut(s) 4, 139, 334
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 2 cut(s) 216, 609
MlyI GAGTC 3 cut(s) 104, 557, 681
MmeI TCCRAC 1 cut(s) 475
MnlI CCTC 2 cut(s) 19, 397
Mph1103I ATGCAT 1 cut(s) 709
MroNI GCCGGC 1 cut(s) 126
MseI TTAA 2 cut(s) 552, 730
MslI CAYNNNNRTG 1 cut(s) 689
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 1 cut(s) 127
MwoI GCNNNNNNNGC 1 cut(s) 127
NaeI GCCGGC 1 cut(s) 128
NdeI CATATG 1 cut(s) 283
NdeII GATC 8 cut(s) 4, 39, 102, 139, 220, 264, 334, 445
NgoMIV GCCGGC 1 cut(s) 126
NlaIII CATG 1 cut(s) 482
NlaIV GGNNCC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 709
PagI TCATGA 1 cut(s) 478
PdiI GCCGGC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 237
PflMI CCANNNNNTGG 1 cut(s) 433
PkrI GCNGC 2 cut(s) 132, 511
PleI GAGTC 3 cut(s) 104, 557, 680
PpsI GAGTC 3 cut(s) 104, 557, 680
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 3 cut(s) 4, 139, 334
PvuII CAGCTG 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 689
SaqAI TTAA 2 cut(s) 552, 730
SatI GCNGC 2 cut(s) 131, 510
Sau3AI GATC 8 cut(s) 4, 39, 102, 139, 220, 264, 334, 445
SchI GAGTC 3 cut(s) 104, 557, 681
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 2 cut(s) 144, 334
SmiMI CAYNNNNRTG 1 cut(s) 689
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
Sse9I AATT 2 cut(s) 216, 609
SsiI CCGC 2 cut(s) 35, 157
TaiI ACGT 1 cut(s) 63
TaqI TCGA 4 cut(s) 42, 77, 113, 456
TasI AATT 2 cut(s) 216, 609
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 2 cut(s) 552, 730
Tru9I TTAA 2 cut(s) 552, 730
TscAI CASTG 1 cut(s) 151
TseI GCWGC 2 cut(s) 130, 509
TspDTI ATGAA 1 cut(s) 467
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 433
ZraI GACGTC 1 cut(s) 61
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.