Rorug07G0098300

Novel plant snare

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0013342
Physical Location & Seq
Forward (+)
7736277 .. 7736935
659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0098300.1

Sequence Viewer

Length: 558 bp
ATGGATATCGTGAAGGGAAAGTGGACGAAGAATCTGATCATCAAGGCGTGGAAGCGATGCAGTACTTTACCGAAACAAAGCAGCAGGAAAGGCTCTACAGCAGCCGCTTCATTGACGAGGAGCAAATCATGGAGCTCCAGTACTACTAGTAGTACTGGCACTTGCAAGCAGAAGAAGAACAAGGCCAAGACATGCCAAGTCGCTCCAGTTGGGTGTTTTATGGTCTATGTTGGACCCGAAAAGCAGCGATTTGTGGTGAGGATGGAGTATGTTAATCATCCATTGTTCAAGATGCTGCTGGAGGATGCAGCATTGGAGTATGGGTACAAAAGTGACGGTCCGATATTGCTTCCTTGCCATGTGGATCTGTTCTGCAATGTTATGGCAGAGATGGAGAGCAATGATATCGATGAGGACAAAATCAGTGCTCCCAGTAATTGTTCTCCGATAATCTTCAGTCCAGCTCGTCGTAGAAATTGTGCCAGCAACAGAGGTTACGGTGGTGCTTATAGGGTACTTACTCCAACATCGTCGTCATCATCAATGGTGCTTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec20

Protein Analysis

185

Amino Acids

20.41

Weight (kDa)

9.53

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 43 - 129 2.1e-24 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35190
fragaria_vesca FvH4_5g20760 FvH4_5g20760
malus_domestica MD04G1053700.v1.1 MD11G1036500.v1.1
prunus_persica Prupe.5G052300_v2.0.a1 Prupe.5G052300_v2.0.a1
pyrus_communis pycom04g04680 pycom11g03010
rosa_chinensis RchiOBHm_Chr7g0206541
rosa_laevigata RLG00000003304
rosa_multiflora Rmu_sc0005065.1_g000056
rosa_roxburghii Rroxscaffold_3G00251420
rosa_rugosa Rorug07G0098300 Rorug07G0098400
rosa_samantha Rh7AG229800 Rh7CG244700
rosa_wichuraiana Rw7G019810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 105
AclWI GGATC 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 439
AfaI GTAC 5 cut(s) 64, 142, 154, 326, 516
AgsI TTSAA 1 cut(s) 289
AhlI ACTAGT 1 cut(s) 146
AluBI AGCT 2 cut(s) 135, 464
AluI AGCT 2 cut(s) 135, 464
Alw21I GWGCWC 2 cut(s) 137, 430
AlwI GGATC 1 cut(s) 372
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 5 cut(s) 81, 101, 244, 295, 308
AspS9I GGNCC 2 cut(s) 233, 338
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 2 cut(s) 233, 338
BaeI ACNNNNGTAYC 2 cut(s) 316, 349
BanII GRGCYC 1 cut(s) 137
Bbv12I GWGCWC 2 cut(s) 137, 430
BbvI GCAGC 5 cut(s) 93, 113, 256, 282, 320
BccI CCATC 2 cut(s) 256, 385
BcgI CGANNNNNNTGC 2 cut(s) 388, 422
BclI TGATCA 1 cut(s) 36
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 2 cut(s) 147, 556
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
BlsI GCNGC 6 cut(s) 83, 103, 106, 246, 297, 310
BmcAI AGTACT 3 cut(s) 64, 142, 154
Bme18I GGWCC 2 cut(s) 233, 338
BmgT120I GGNCC 2 cut(s) 233, 338
BmiI GGNNCC 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 3 cut(s) 47, 282, 295
BmuI ACTGGG 1 cut(s) 426
BpmI CTGGAG 3 cut(s) 121, 189, 320
Bsa29I ATCGAT 1 cut(s) 408
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 308, 338
Bse1I ACTGG 4 cut(s) 138, 160, 206, 432
Bse3DI GCAATG 2 cut(s) 382, 406
BseCI ATCGAT 1 cut(s) 408
BseGI GGATG 3 cut(s) 267, 277, 310
BseMI GCAATG 2 cut(s) 382, 406
BseNI ACTGG 4 cut(s) 138, 160, 206, 432
BseRI GAGGAG 1 cut(s) 133
BseXI GCAGC 5 cut(s) 93, 113, 256, 282, 320
BshFI GGCC 1 cut(s) 185
BshVI ATCGAT 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 137, 430
BsnI GGCC 1 cut(s) 185
Bsp1286I GDGCHC 2 cut(s) 137, 430
Bsp143I GATC 2 cut(s) 36, 364
BspACI CCGC 1 cut(s) 105
BspANI GGCC 1 cut(s) 185
BspDI ATCGAT 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 235
BspPI GGATC 1 cut(s) 372
BsrDI GCAATG 2 cut(s) 382, 406
BsrI ACTGG 4 cut(s) 138, 160, 206, 432
BssMI GATC 2 cut(s) 36, 364
Bst4CI ACNGT 2 cut(s) 338, 500
BstC8I GCNNGC 2 cut(s) 167, 484
BstF5I GGATG 3 cut(s) 267, 277, 310
BstKTI GATC 2 cut(s) 39, 367
BstMBI GATC 2 cut(s) 36, 364
BstMWI GCNNNNNNNGC 1 cut(s) 90
BstNSI RCATGY 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 5 cut(s) 93, 113, 256, 282, 320
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 1 cut(s) 185
BsuTUI ATCGAT 1 cut(s) 408
BtgZI GCGATG 1 cut(s) 70
BtsCI GGATG 3 cut(s) 267, 277, 310
BtsIMutI CAGTG 1 cut(s) 430
Cac8I GCNNGC 2 cut(s) 167, 484
Cfr13I GGNCC 2 cut(s) 233, 338
ClaI ATCGAT 1 cut(s) 408
CpoI CGGWCCG 1 cut(s) 338
Csp6I GTAC 5 cut(s) 63, 141, 153, 325, 515
CspI CGGWCCG 1 cut(s) 338
CviAII CATG 3 cut(s) 129, 192, 359
CviJI RGCY 5 cut(s) 93, 104, 135, 185, 464
CviKI_1 RGCY 5 cut(s) 93, 104, 135, 185, 464
CviQI GTAC 5 cut(s) 63, 141, 153, 325, 515
DpnI GATC 2 cut(s) 38, 366
DpnII GATC 2 cut(s) 36, 364
Ecl136II GAGCTC 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 137
Eco32I GATATC 2 cut(s) 7, 406
Eco47I GGWCC 2 cut(s) 233, 338
Eco53kI GAGCTC 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 439
EcoICRI GAGCTC 1 cut(s) 135
EcoRV GATATC 2 cut(s) 7, 406
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 3 cut(s) 132, 195, 362
FaiI YATR 9 cut(s) 130, 193, 221, 228, 270, 321, 360, 383, 510
FatI CATG 3 cut(s) 128, 191, 358
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
FokI GGATG 3 cut(s) 264, 274, 317
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
FspBI CTAG 2 cut(s) 147, 556
GluI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
GsuI CTGGAG 3 cut(s) 121, 189, 320
HaeIII GGCC 1 cut(s) 185
Hin1II CATG 3 cut(s) 132, 195, 362
HinfI GANTC 1 cut(s) 31
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 36, 342, 447
Hpy188III TCNNGA 2 cut(s) 10, 289
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 2 cut(s) 471, 535
HpyAV CCTTC 1 cut(s) 7
HpyCH4III ACNGT 2 cut(s) 338, 500
HpyCH4V TGCA 4 cut(s) 60, 165, 308, 375
HpyF10VI GCNNNNNNNGC 1 cut(s) 90
Hsp92II CATG 3 cut(s) 132, 195, 362
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 2 cut(s) 36, 364
LmnI GCTCC 5 cut(s) 120, 132, 140, 208, 433
LpnPI CCDG 8 cut(s) 70, 141, 151, 219, 284, 445, 474, 496
Lsp1109I GCAGC 5 cut(s) 93, 113, 256, 282, 320
LweI GCATC 3 cut(s) 47, 282, 295
MaeI CTAG 2 cut(s) 147, 556
MaeIII GTNAC 2 cut(s) 332, 494
MalI GATC 2 cut(s) 38, 366
MboI GATC 2 cut(s) 36, 364
MboII GAAGA 4 cut(s) 40, 184, 187, 445
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 2 cut(s) 137, 430
MluCI AATT 2 cut(s) 436, 475
MmeI TCCRAC 2 cut(s) 211, 548
MnlI CCTC 5 cut(s) 111, 252, 295, 406, 485
MseI TTAA 1 cut(s) 273
MwoI GCNNNNNNNGC 1 cut(s) 90
NdeII GATC 2 cut(s) 36, 364
NlaIII CATG 3 cut(s) 132, 195, 362
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 1 cut(s) 195
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 6 cut(s) 83, 103, 106, 246, 297, 310
Psp124BI GAGCTC 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 2 cut(s) 233, 338
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 5 cut(s) 64, 142, 154, 326, 516
RsaNI GTAC 5 cut(s) 63, 141, 153, 325, 515
Rsr2I CGGWCCG 1 cut(s) 338
RsrII CGGWCCG 1 cut(s) 338
SacI GAGCTC 1 cut(s) 137
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
Sau3AI GATC 2 cut(s) 36, 364
Sau96I GGNCC 2 cut(s) 233, 338
ScaI AGTACT 3 cut(s) 64, 142, 154
SduI GDGCHC 2 cut(s) 137, 430
SetI ASST 4 cut(s) 137, 466, 496, 557
SfaNI GCATC 3 cut(s) 47, 282, 295
SfcI CTRYAG 1 cut(s) 96
SinI GGWCC 2 cut(s) 233, 338
SpeI ACTAGT 1 cut(s) 146
Sse9I AATT 2 cut(s) 436, 475
SsiI CCGC 1 cut(s) 105
SspMI CTAG 2 cut(s) 147, 556
SstI GAGCTC 1 cut(s) 137
TaaI ACNGT 2 cut(s) 338, 500
TaqI TCGA 1 cut(s) 408
TasI AATT 2 cut(s) 436, 475
TatI WGTACW 3 cut(s) 62, 140, 152
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 5 cut(s) 81, 101, 244, 295, 308
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 99
TspRI CASTG 1 cut(s) 430
VpaK11BI GGWCC 2 cut(s) 233, 338
XceI RCATGY 1 cut(s) 195
XspI CTAG 2 cut(s) 147, 556
ZrmI AGTACT 3 cut(s) 64, 142, 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.