FvH4_5g21680

TLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015142
Physical Location & Seq
Reverse (-)
13094477 .. 13096021
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21680.t1

Sequence Viewer

Length: 708 bp
ATGGCTTATTGCTTCTCAACCACTGTCTCTTATGGTTCTGGATGGAAGAGAAGCCCATATAGCTTGTTTGGGTGGAACATAGGGAAACCAAAACTTGATGAAGATCGAAGGCCACAGATCAATTACCATGATACTACTGATCTTCCATTTCCACCTTCTCTTCTGGACAAAACATTCTTAAGAGGTAGGGAACTCAAATGTTGCTACAAGGCAACCATTGATGGATTTAGTGCAGCTGATTTCCACAATTGCTGTGATTTCAAGGGGCCATGTGCCATAATTGGCTATACAAACAAGTCCTTCAAGTTTGGTGCATTCAACCCTGAAGGCTATAGAAGCACAGATGATTACTATGACAGTTTTGATCCATTCCTATTCTACTGGACAGATGATGAAAAAACTGATGATCCAATTGTTCTGCCCAAAGTAGGAGGCAGTGGTGCAGCCCTTTTTGATTATGCTCGGGGCGGGCCACAATTTGGGGCTGATGGGCTGCTCATCGGCCCACCTCTAGCGCCGGTTATGGGTGGGTTTGCTGGCCCGGATACTAATTCAGGTATTGGTGATTTAAGGCAAGCCAAATCTAGATTAGGATTGTCATATGCAAAGAGAAAAGATGGGAAGGAGTCACTTTTCGGGGATGAGAATAGAGCAACCCTTGAAGAAGTAGAAGTTTACTGTAGTCCTCAAATTGCAAGCTTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TLD

Protein Analysis

236

Amino Acids

26.0

Weight (kDa)

5.13

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TLD PF07534 60 - 226 7.6e-10 TLDc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32520
fragaria_vesca FvH4_5g21680
malus_domestica MD06G1031800.v1.1
prunus_persica Prupe.5G040300_v2.0.a1
pyrus_communis pycom06g02640
rosa_chinensis RchiOBHm_Chr7g0209181
rosa_laevigata RLG00000000565 RLG00000003159
rosa_multiflora Rmu_sc0001701.1_g000004
rosa_roxburghii Rroxscaffold_3G00249660
rosa_rugosa Rorug07G0110000
rosa_samantha Rh7BG239300 Rh7CG259600 Rh7DG250900 Rh7DG484700
rosa_wichuraiana Rw7G020700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 479
AciI CCGC 1 cut(s) 468
AclWI GGATC 2 cut(s) 359, 401
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 1 cut(s) 704
AfiI CCNNNNNNNGG 3 cut(s) 428, 479, 524
AflII CTTAAG 1 cut(s) 178
AgsI TTSAA 4 cut(s) 262, 304, 319, 662
AluBI AGCT 3 cut(s) 63, 236, 699
AluI AGCT 3 cut(s) 63, 236, 699
Alw26I GTCTC 1 cut(s) 31
AlwI GGATC 2 cut(s) 359, 401
Ama87I CYCGRG 1 cut(s) 462
AoxI GGCC 5 cut(s) 110, 266, 470, 502, 538
ApeKI GCWGC 3 cut(s) 233, 443, 493
AspLEI GCGC 1 cut(s) 517
AspS9I GGNCC 4 cut(s) 266, 470, 503, 539
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 1 cut(s) 575
AvaI CYCGRG 1 cut(s) 462
BbvI GCAGC 3 cut(s) 245, 455, 480
BccI CCATC 4 cut(s) 36, 215, 482, 611
BciVI GTATCC 1 cut(s) 538
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 1 cut(s) 31
BfaI CTAG 2 cut(s) 512, 585
BfmI CTRYAG 2 cut(s) 331, 679
BfoI RGCGCY 1 cut(s) 518
BfrI CTTAAG 1 cut(s) 178
BfuI GTATCC 1 cut(s) 538
BisI GCNGC 3 cut(s) 234, 444, 494
BlsI GCNGC 3 cut(s) 235, 445, 495
Bme1390I CCNGG 1 cut(s) 542
BmeT110I CYCGRG 1 cut(s) 462
BmgT120I GGNCC 4 cut(s) 266, 470, 503, 539
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 542
BsaBI GATNNNNATC 1 cut(s) 102
Bsc4I CCNNNNNNNGG 3 cut(s) 428, 479, 524
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 1 cut(s) 386
Bse8I GATNNNNATC 1 cut(s) 102
BseGI GGATG 2 cut(s) 47, 646
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 3 cut(s) 428, 479, 524
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 3 cut(s) 245, 455, 480
BsgI GTGCAG 2 cut(s) 252, 462
BshFI GGCC 5 cut(s) 112, 268, 472, 504, 540
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 2 cut(s) 518, 542
BslI CCNNNNNNNGG 3 cut(s) 428, 479, 524
BsmAI GTCTC 1 cut(s) 31
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 5 cut(s) 112, 268, 472, 504, 540
BsoBI CYCGRG 1 cut(s) 462
Bsp143I GATC 5 cut(s) 103, 117, 139, 364, 406
BspACI CCGC 1 cut(s) 468
BspANI GGCC 5 cut(s) 112, 268, 472, 504, 540
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 2 cut(s) 359, 401
BspTI CTTAAG 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 517
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 517
BssMI GATC 5 cut(s) 103, 117, 139, 364, 406
Bst4CI ACNGT 3 cut(s) 25, 359, 680
Bst6I CTCTTC 2 cut(s) 41, 165
BstAFI CTTAAG 1 cut(s) 178
BstC8I GCNNGC 4 cut(s) 470, 538, 576, 697
BstF5I GGATG 2 cut(s) 47, 646
BstH2I RGCGCY 1 cut(s) 518
BstHHI GCGC 1 cut(s) 517
BstKTI GATC 5 cut(s) 106, 120, 142, 367, 409
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 5 cut(s) 103, 117, 139, 364, 406
BstMWI GCNNNNNNNGC 2 cut(s) 60, 336
BstSCI CCNGG 1 cut(s) 540
BstSFI CTRYAG 2 cut(s) 331, 679
BstV1I GCAGC 3 cut(s) 245, 455, 480
BsuI GTATCC 1 cut(s) 538
BsuRI GGCC 5 cut(s) 112, 268, 472, 504, 540
BtsCI GGATG 2 cut(s) 47, 646
BtsI GCAGTG 1 cut(s) 442
BtsIMutI CAGTG 2 cut(s) 21, 442
Cac8I GCNNGC 4 cut(s) 470, 538, 576, 697
CfoI GCGC 1 cut(s) 517
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 4 cut(s) 266, 470, 503, 539
Csp6I GTAC 1 cut(s) 703
CviAII CATG 2 cut(s) 128, 270
CviQI GTAC 1 cut(s) 703
DpnI GATC 5 cut(s) 105, 119, 141, 366, 408
DpnII GATC 5 cut(s) 103, 117, 139, 364, 406
Eam1104I CTCTTC 2 cut(s) 41, 165
EarI CTCTTC 2 cut(s) 41, 165
Eco57I CTGAAG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 462
FaeI CATG 2 cut(s) 131, 273
FatI CATG 2 cut(s) 127, 269
FauI CCCGC 1 cut(s) 461
FauNDI CATATG 1 cut(s) 601
Fnu4HI GCNGC 3 cut(s) 234, 444, 494
FokI GGATG 2 cut(s) 54, 653
Fsp4HI GCNGC 3 cut(s) 234, 444, 494
FspBI CTAG 2 cut(s) 512, 585
GlaI GCGC 1 cut(s) 516
GluI GCNGC 3 cut(s) 234, 444, 494
HaeII RGCGCY 1 cut(s) 518
HaeIII GGCC 5 cut(s) 112, 268, 472, 504, 540
HapII CCGG 2 cut(s) 518, 542
HhaI GCGC 1 cut(s) 517
Hin1II CATG 2 cut(s) 131, 273
Hin6I GCGC 1 cut(s) 515
HinP1I GCGC 1 cut(s) 515
HindIII AAGCTT 1 cut(s) 697
HinfI GANTC 1 cut(s) 626
HpaII CCGG 2 cut(s) 518, 542
HphI GGTGA 1 cut(s) 575
Hpy166II GTNNAC 1 cut(s) 676
Hpy188III TCNNGA 3 cut(s) 39, 164, 585
Hpy8I GTNNAC 1 cut(s) 676
HpyAV CCTTC 5 cut(s) 102, 165, 310, 320, 616
HpyCH4III ACNGT 3 cut(s) 25, 359, 680
HpyCH4V TGCA 5 cut(s) 233, 314, 443, 605, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 336
Hsp92II CATG 2 cut(s) 131, 273
HspAI GCGC 1 cut(s) 515
Kzo9I GATC 5 cut(s) 103, 117, 139, 364, 406
LpnPI CCDG 8 cut(s) 24, 149, 336, 367, 522, 531, 540, 555
Lsp1109I GCAGC 3 cut(s) 245, 455, 480
MaeI CTAG 2 cut(s) 512, 585
MaeIII GTNAC 1 cut(s) 627
MalI GATC 5 cut(s) 105, 119, 141, 366, 408
MboI GATC 5 cut(s) 103, 117, 139, 364, 406
MboII GAAGA 5 cut(s) 58, 113, 134, 152, 674
MfeI CAATTG 2 cut(s) 247, 411
MluCI AATT 7 cut(s) 121, 247, 279, 411, 476, 550, 690
MlyI GAGTC 1 cut(s) 635
MnlI CCTC 4 cut(s) 176, 425, 519, 696
MseI TTAA 2 cut(s) 179, 569
MspA1I CMGCKG 1 cut(s) 236
MspCI CTTAAG 1 cut(s) 178
MspI CCGG 2 cut(s) 518, 542
MspR9I CCNGG 1 cut(s) 542
MunI CAATTG 2 cut(s) 247, 411
Mva1269I GAATGC 1 cut(s) 314
MwoI GCNNNNNNNGC 2 cut(s) 60, 336
NciI CCSGG 1 cut(s) 542
NdeI CATATG 1 cut(s) 601
NdeII GATC 5 cut(s) 103, 117, 139, 364, 406
NlaIII CATG 2 cut(s) 131, 273
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 627
PctI GAATGC 1 cut(s) 314
PflMI CCANNNNNTGG 1 cut(s) 479
PkrI GCNGC 3 cut(s) 235, 445, 495
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 4 cut(s) 266, 470, 503, 539
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 1 cut(s) 704
RsaNI GTAC 1 cut(s) 703
SaqAI TTAA 2 cut(s) 179, 569
SatI GCNGC 3 cut(s) 234, 444, 494
Sau3AI GATC 5 cut(s) 103, 117, 139, 364, 406
Sau96I GGNCC 4 cut(s) 266, 470, 503, 539
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 1 cut(s) 542
SetI ASST 7 cut(s) 65, 157, 187, 238, 511, 559, 701
SfcI CTRYAG 2 cut(s) 331, 679
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse9I AATT 7 cut(s) 121, 247, 279, 411, 476, 550, 690
SsiI CCGC 1 cut(s) 468
SspMI CTAG 2 cut(s) 512, 585
StyD4I CCNGG 1 cut(s) 540
TaaI ACNGT 3 cut(s) 25, 359, 680
TaqI TCGA 1 cut(s) 106
TasI AATT 7 cut(s) 121, 247, 279, 411, 476, 550, 690
TatI WGTACW 1 cut(s) 702
Tru1I TTAA 2 cut(s) 179, 569
Tru9I TTAA 2 cut(s) 179, 569
TscAI CASTG 2 cut(s) 28, 442
TseFI GTSAC 1 cut(s) 627
TseI GCWGC 3 cut(s) 233, 443, 493
Tsp45I GTSAC 1 cut(s) 627
TspDTI ATGAA 2 cut(s) 114, 408
TspRI CASTG 2 cut(s) 28, 442
Van91I CCANNNNNTGG 1 cut(s) 479
Vha464I CTTAAG 1 cut(s) 178
XbaI TCTAGA 1 cut(s) 584
XspI CTAG 2 cut(s) 512, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.