MD06G1031800.v1.1

TLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015142
Physical Location & Seq
Reverse (-)
3837884 .. 3839027
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1031800.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGCTTGTTGCTTCTCAAACACTGTATCTTATGGCTGGAAACTGAAAAGAAGCAGCCCATACAGCTTGTTTGGCTGGAGTATAGGAAAAACCAAACAGGATGAAATGCCACAGACCAAGTACTATGACACTGATCTTCCATTCCCACCTTCTCTGCTTGACAAAACATTCTTAAGTGGTAGGGAACTCAAGTGTTGCTATAAGGCCACAATTGATGGATTTAGTGCAGCTAATTTCCACAACTCCTGTGACTTCAAGGGGCCATGTGCCATAATTGGCTACACAAACAAGTCCTTCAAGTTTGGTGCATTCAACCCTGAAGGCTACAGAAGCACAGACGATTATTACGACACCTTCGATGCATTCCTTTTCTACTGGACAGACAATGACATAACTGATCCAATTGTTTTGCCCAAAGTAGGAGGAAGTGGTGCAGCCCTTTTCGATTATGCTCGAGGTGGGCCGCAATTTGGGGCCGATGGGCTGCTTATCGGACCACCTCTAGCGCCCGTTATGGGCGGGTTTGCAGGCCCGGATACTAATTCGGGTATTGGTGATTTAAGGCAAGCTAAATCTAGATTAGGATTGTCATATGCAAAGAGGGAAGATGGGAAGGAATCACTTTTTGGTGATGAGTCTAGAGCAACCCTTGAAGAAGTTGAAGTTTATTGTAGTCCTCAAATTGCAAGCTTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TLD

Protein Analysis

233

Amino Acids

25.49

Weight (kDa)

4.98

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TLD PF07534 59 - 179 2.2e-10 TLDc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32520
fragaria_vesca FvH4_5g21680
malus_domestica MD06G1031800.v1.1
prunus_persica Prupe.5G040300_v2.0.a1
pyrus_communis pycom06g02640
rosa_chinensis RchiOBHm_Chr7g0209181
rosa_laevigata RLG00000000565 RLG00000003159
rosa_multiflora Rmu_sc0001701.1_g000004
rosa_roxburghii Rroxscaffold_3G00249660
rosa_rugosa Rorug07G0110000
rosa_samantha Rh7BG239300 Rh7CG259600 Rh7DG250900 Rh7DG484700
rosa_wichuraiana Rw7G020700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 464, 519
AclWI GGATC 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 2 cut(s) 122, 695
AfiI CCNNNNNNNGG 3 cut(s) 419, 470, 515
AflII CTTAAG 1 cut(s) 172
AgsI TTSAA 5 cut(s) 256, 298, 313, 653, 662
AjuI GAANNNNNNNTTGG 2 cut(s) 609, 641
AluBI AGCT 4 cut(s) 66, 230, 569, 690
AluI AGCT 4 cut(s) 66, 230, 569, 690
AlwI GGATC 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 453
AoxI GGCC 5 cut(s) 204, 260, 461, 474, 529
ApeKI GCWGC 4 cut(s) 54, 227, 434, 484
AspLEI GCGC 1 cut(s) 508
AspS9I GGNCC 5 cut(s) 260, 461, 474, 494, 530
AsuC2I CCSGG 1 cut(s) 533
AsuHPI GGTGA 2 cut(s) 566, 641
AvaI CYCGRG 1 cut(s) 453
AvaII GGWCC 1 cut(s) 494
BbvI GCAGC 4 cut(s) 66, 239, 446, 471
BccI CCATC 3 cut(s) 209, 473, 602
BciVI GTATCC 1 cut(s) 529
BcnI CCSGG 1 cut(s) 533
BfaI CTAG 3 cut(s) 503, 576, 639
BfmI CTRYAG 1 cut(s) 325
BfoI RGCGCY 1 cut(s) 509
BfrI CTTAAG 1 cut(s) 172
BfuI GTATCC 1 cut(s) 529
BisI GCNGC 5 cut(s) 55, 228, 435, 464, 485
BlsI GCNGC 5 cut(s) 56, 229, 436, 465, 486
BmcAI AGTACT 1 cut(s) 122
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 1 cut(s) 494
BmeT110I CYCGRG 1 cut(s) 453
BmgT120I GGNCC 5 cut(s) 260, 461, 474, 494, 530
BmiI GGNNCC 2 cut(s) 261, 475
BmrFI CCNGG 1 cut(s) 533
BmsI GCATC 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 533
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 470, 515
Bse1I ACTGG 1 cut(s) 380
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 3 cut(s) 419, 470, 515
BseNI ACTGG 1 cut(s) 380
BseXI GCAGC 4 cut(s) 66, 239, 446, 471
BsgI GTGCAG 2 cut(s) 246, 453
BshFI GGCC 5 cut(s) 206, 262, 463, 476, 531
BsiHKCI CYCGRG 1 cut(s) 453
BsiSI CCGG 1 cut(s) 533
BslI CCNNNNNNNGG 3 cut(s) 419, 470, 515
BsmI GAATGC 2 cut(s) 308, 362
BsnI GGCC 5 cut(s) 206, 262, 463, 476, 531
BsoBI CYCGRG 1 cut(s) 453
Bsp143I GATC 2 cut(s) 133, 397
BspACI CCGC 2 cut(s) 464, 519
BspANI GGCC 5 cut(s) 206, 262, 463, 476, 531
BspLI GGNNCC 2 cut(s) 261, 475
BspPI GGATC 1 cut(s) 392
BspTI CTTAAG 1 cut(s) 172
BsrI ACTGG 1 cut(s) 380
BssMI GATC 2 cut(s) 133, 397
Bst4CI ACNGT 1 cut(s) 25
BstAFI CTTAAG 1 cut(s) 172
BstC8I GCNNGC 3 cut(s) 529, 567, 688
BstF5I GGATG 1 cut(s) 106
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 1 cut(s) 508
BstKTI GATC 2 cut(s) 136, 400
BstMBI GATC 2 cut(s) 133, 397
BstMWI GCNNNNNNNGC 3 cut(s) 63, 72, 330
BstSCI CCNGG 1 cut(s) 531
BstSFI CTRYAG 1 cut(s) 325
BstV1I GCAGC 4 cut(s) 66, 239, 446, 471
BsuI GTATCC 1 cut(s) 529
BsuRI GGCC 5 cut(s) 206, 262, 463, 476, 531
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 2 cut(s) 21, 129
Cac8I GCNNGC 3 cut(s) 529, 567, 688
CfoI GCGC 1 cut(s) 508
Cfr13I GGNCC 5 cut(s) 260, 461, 474, 494, 530
Csp6I GTAC 2 cut(s) 121, 694
CviAII CATG 1 cut(s) 264
CviQI GTAC 2 cut(s) 121, 694
DpnI GATC 2 cut(s) 135, 399
DpnII GATC 2 cut(s) 133, 397
Eco47I GGWCC 1 cut(s) 494
Eco57I CTGAAG 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 453
EcoT22I ATGCAT 1 cut(s) 364
FaeI CATG 1 cut(s) 267
FatI CATG 1 cut(s) 263
FauI CCCGC 1 cut(s) 512
FauNDI CATATG 1 cut(s) 592
Fnu4HI GCNGC 5 cut(s) 55, 228, 435, 464, 485
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 5 cut(s) 55, 228, 435, 464, 485
FspBI CTAG 3 cut(s) 503, 576, 639
GlaI GCGC 1 cut(s) 507
GluI GCNGC 5 cut(s) 55, 228, 435, 464, 485
GsuI CTGGAG 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 5 cut(s) 206, 262, 463, 476, 531
HapII CCGG 1 cut(s) 533
HhaI GCGC 1 cut(s) 508
Hin1II CATG 1 cut(s) 267
Hin6I GCGC 1 cut(s) 506
HinP1I GCGC 1 cut(s) 506
HindIII AAGCTT 1 cut(s) 688
HinfI GANTC 2 cut(s) 617, 635
HpaII CCGG 1 cut(s) 533
HphI GGTGA 2 cut(s) 566, 641
Hpy188I TCNGA 1 cut(s) 494
Hpy188III TCNNGA 2 cut(s) 576, 639
HpyAV CCTTC 5 cut(s) 159, 304, 314, 364, 607
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 7 cut(s) 227, 308, 362, 434, 527, 596, 686
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 72, 330
Hsp92II CATG 1 cut(s) 267
HspAI GCGC 1 cut(s) 506
Kzo9I GATC 2 cut(s) 133, 397
LpnPI CCDG 8 cut(s) 22, 61, 83, 259, 330, 361, 513, 546
Lsp1109I GCAGC 4 cut(s) 66, 239, 446, 471
LweI GCATC 1 cut(s) 349
MaeI CTAG 3 cut(s) 503, 576, 639
MaeIII GTNAC 1 cut(s) 248
MalI GATC 2 cut(s) 135, 399
MboI GATC 2 cut(s) 133, 397
MboII GAAGA 3 cut(s) 128, 617, 665
MfeI CAATTG 2 cut(s) 210, 402
MluCI AATT 7 cut(s) 210, 232, 273, 402, 467, 541, 681
MlyI GAGTC 1 cut(s) 644
MnlI CCTC 5 cut(s) 416, 449, 510, 594, 687
Mph1103I ATGCAT 1 cut(s) 364
MseI TTAA 2 cut(s) 173, 560
MspCI CTTAAG 1 cut(s) 172
MspI CCGG 1 cut(s) 533
MspR9I CCNGG 1 cut(s) 533
MunI CAATTG 2 cut(s) 210, 402
Mva1269I GAATGC 2 cut(s) 308, 362
MwoI GCNNNNNNNGC 3 cut(s) 63, 72, 330
NciI CCSGG 1 cut(s) 533
NdeI CATATG 1 cut(s) 592
NdeII GATC 2 cut(s) 133, 397
NlaIII CATG 1 cut(s) 267
NlaIV GGNNCC 2 cut(s) 261, 475
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 364
PaeR7I CTCGAG 1 cut(s) 453
PcsI WCGNNNNNNNCGW 2 cut(s) 345, 354
PctI GAATGC 2 cut(s) 308, 362
PfeI GAWTC 1 cut(s) 617
PkrI GCNGC 5 cut(s) 56, 229, 436, 465, 486
PleI GAGTC 1 cut(s) 643
PpsI GAGTC 1 cut(s) 643
PspN4I GGNNCC 2 cut(s) 261, 475
PspPI GGNCC 5 cut(s) 260, 461, 474, 494, 530
PspXI VCTCGAGB 1 cut(s) 453
RsaI GTAC 2 cut(s) 122, 695
RsaNI GTAC 2 cut(s) 121, 694
SaqAI TTAA 2 cut(s) 173, 560
SatI GCNGC 5 cut(s) 55, 228, 435, 464, 485
Sau3AI GATC 2 cut(s) 133, 397
Sau96I GGNCC 5 cut(s) 260, 461, 474, 494, 530
ScaI AGTACT 1 cut(s) 122
SchI GAGTC 1 cut(s) 644
ScrFI CCNGG 1 cut(s) 533
SetI ASST 8 cut(s) 68, 151, 232, 356, 460, 502, 571, 692
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 325
Sfr274I CTCGAG 1 cut(s) 453
SinI GGWCC 1 cut(s) 494
SlaI CTCGAG 1 cut(s) 453
SmlI CTYRAG 3 cut(s) 172, 188, 453
SmoI CTYRAG 3 cut(s) 172, 188, 453
Sse9I AATT 7 cut(s) 210, 232, 273, 402, 467, 541, 681
SsiI CCGC 2 cut(s) 464, 519
SspMI CTAG 3 cut(s) 503, 576, 639
StyD4I CCNGG 1 cut(s) 531
TaaI ACNGT 1 cut(s) 25
TaqI TCGA 3 cut(s) 357, 444, 454
TasI AATT 7 cut(s) 210, 232, 273, 402, 467, 541, 681
TatI WGTACW 2 cut(s) 120, 693
TauI GCSGC 1 cut(s) 466
TfiI GAWTC 1 cut(s) 617
Tru1I TTAA 2 cut(s) 173, 560
Tru9I TTAA 2 cut(s) 173, 560
TscAI CASTG 2 cut(s) 28, 136
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 4 cut(s) 54, 227, 434, 484
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 1 cut(s) 117
TspRI CASTG 2 cut(s) 28, 136
Vha464I CTTAAG 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 494
XbaI TCTAGA 2 cut(s) 575, 638
XhoI CTCGAG 1 cut(s) 453
XspI CTAG 3 cut(s) 503, 576, 639
ZrmI AGTACT 1 cut(s) 122
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.