FvH4_5g22470

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014316
Physical Location & Seq
Reverse (-)
13822087 .. 13825225
3139 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22470.t1

Sequence Viewer

Length: 780 bp
ATGGGGAAGATAGAGAACAACAATGAGGAAGGGGAAGGGGATGTGAAGGGATCTATCAGTAAATTCCCATTGAACTTTTGGGAGATGACGGTGGCTTCCACGGTGGTGCTGGGTTTTCTGTTCGGCCTTATGGGTGTTTACCTAACCATGCCCGCATCCGATTACAGCTTTCTCAAGCTCCCCCGGACCCTCCAAGACCTCCAAATCCTAAGAGATCACCTTGAAGACTACACAAGTGACTACACAGCTCAGGTTTTGTTAGGGTACTGTATGGTTTATATATTCATGCAGACATTCATGATTCCTGGAACTGTGTTCATGTCGTTGCTTGCTGGTTCCCTTTTTGGAGTCTTCAGAGGTGTAGCTTTGGTTGTGTTCAATGCCACTGCTGGTGCTTCTTCTTGTTATTTCTTGTCAAAGGTGATAGGGAGGCCCCTTGCCACCTCTCTTTGGCCCGACAAGCTCCTTTTCTTCCAAAACCAGGTGGCTAAAAGAAGAGCGCGGCTGTTGAACTACATGTTGTTTCTCCGATTAACTCCAACCTTGCCAAACACATTTATTAATGTTGCTTCACCAATTGTTGATGTGCCTTATCATACATTCTTCCTGGCAACCTCAATTGGTCTGATACCTGCTGCTTACGTCACTGTCAGGGCTGGACTAGCTCTCGGAGAATTGCGTTCGCTTGGAGATCTCTACGACTTCAACTCAATAGCCACCTTGTTCTTCATTGGAGTTGTCTCGGTTACTCCCACGCTAGTGAGCAAGAACAAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

260

Amino Acids

28.66

Weight (kDa)

8.47

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 101 - 220 1.3e-14 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17790
fragaria_vesca FvH4_5g22470
malus_domestica MD06G1037100.v1.1 MD16G1272300.v1.1
prunus_persica Prupe.5G032200_v2.0.a1
pyrus_communis pycom16g24130
rosa_chinensis RchiOBHm_Chr7g0210261
rosa_laevigata RLG00000003054
rosa_multiflora Rmu_sc0017046.1_g000008
rosa_roxburghii Rroxscaffold_3G00248510
rosa_rugosa Rorug07G0120100
rosa_samantha Rh7AG253000 Rh7BG248100 Rh7CG270000 Rh7DG260600
rosa_wichuraiana Rw7G021520 Rw7G021570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 640
AccII CGCG 1 cut(s) 502
AciI CCGC 2 cut(s) 153, 502
AclWI GGATC 1 cut(s) 58
AcsI RAATTY 1 cut(s) 62
AcuI CTGAAG 1 cut(s) 337
AfaI GTAC 1 cut(s) 266
AfiI CCNNNNNNNGG 2 cut(s) 450, 481
AflIII ACRYGT 1 cut(s) 516
AgsI TTSAA 5 cut(s) 73, 224, 379, 511, 706
AjnI CCWGG 3 cut(s) 304, 480, 606
AleI CACNNNNGTG 2 cut(s) 104, 758
AluBI AGCT 6 cut(s) 168, 178, 248, 365, 463, 665
AluI AGCT 6 cut(s) 168, 178, 248, 365, 463, 665
Alw26I GTCTC 1 cut(s) 745
AlwI GGATC 1 cut(s) 58
AoxI GGCC 3 cut(s) 124, 431, 452
ApeKI GCWGC 1 cut(s) 635
ApoI RAATTY 1 cut(s) 62
AseI ATTAAT 1 cut(s) 561
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 3 cut(s) 186, 432, 453
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 3 cut(s) 209, 433, 564
AvaII GGWCC 1 cut(s) 186
BbsI GAAGAC 2 cut(s) 231, 343
BbvI GCAGC 1 cut(s) 622
BciT130I CCWGG 3 cut(s) 306, 482, 608
BcnI CCSGG 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 745
BfaI CTAG 2 cut(s) 662, 758
BfuAI ACCTGC 1 cut(s) 640
BglII AGATCT 1 cut(s) 691
BisI GCNGC 2 cut(s) 503, 636
BlsI GCNGC 2 cut(s) 504, 637
Bme1390I CCNGG 4 cut(s) 184, 306, 482, 608
Bme18I GGWCC 1 cut(s) 186
BmgT120I GGNCC 3 cut(s) 186, 432, 453
BmiI GGNNCC 3 cut(s) 188, 337, 434
BmrFI CCNGG 4 cut(s) 184, 306, 482, 608
BmsI GCATC 1 cut(s) 164
BpiI GAAGAC 2 cut(s) 231, 343
Bpu10I CCTNAGC 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 184
BsaJI CCNNGG 2 cut(s) 99, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 450, 481
BseBI CCWGG 3 cut(s) 306, 482, 608
BseDI CCNNGG 2 cut(s) 99, 182
BseGI GGATG 2 cut(s) 46, 155
BseLI CCNNNNNNNGG 2 cut(s) 450, 481
BseMII CTCAG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 622
BseYI CCCAGC 1 cut(s) 109
Bsh1236I CGCG 1 cut(s) 502
BshFI GGCC 3 cut(s) 126, 433, 454
BsiSI CCGG 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 450, 481
BsmAI GTCTC 1 cut(s) 745
BsnI GGCC 3 cut(s) 126, 433, 454
Bsp143I GATC 3 cut(s) 50, 214, 691
BspACI CCGC 2 cut(s) 153, 502
BspANI GGCC 3 cut(s) 126, 433, 454
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 1 cut(s) 502
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 3 cut(s) 188, 337, 434
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 1 cut(s) 58
BspQI GCTCTTC 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 99, 182
BssMI GATC 3 cut(s) 50, 214, 691
Bst2UI CCWGG 3 cut(s) 306, 482, 608
Bst4CI ACNGT 5 cut(s) 91, 103, 269, 313, 649
Bst6I CTCTTC 1 cut(s) 490
BstC8I GCNNGC 2 cut(s) 153, 330
BstDEI CTNAG 2 cut(s) 209, 249
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 2 cut(s) 46, 155
BstFNI CGCG 1 cut(s) 502
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 3 cut(s) 53, 217, 694
BstMAI GTCTC 1 cut(s) 745
BstMBI GATC 3 cut(s) 50, 214, 691
BstMWI GCNNNNNNNGC 2 cut(s) 460, 662
BstNI CCWGG 3 cut(s) 306, 482, 608
BstNSI RCATGY 1 cut(s) 520
BstSCI CCNGG 4 cut(s) 182, 304, 480, 606
BstUI CGCG 1 cut(s) 502
BstV1I GCAGC 1 cut(s) 622
BstV2I GAAGAC 2 cut(s) 231, 343
BstX2I RGATCY 2 cut(s) 50, 691
BstYI RGATCY 2 cut(s) 50, 691
BsuRI GGCC 3 cut(s) 126, 433, 454
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 2 cut(s) 46, 155
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 2 cut(s) 384, 645
BveI ACCTGC 1 cut(s) 640
Cac8I GCNNGC 2 cut(s) 153, 330
CciI TCATGA 1 cut(s) 297
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 3 cut(s) 186, 432, 453
CsiI ACCWGGT 1 cut(s) 480
Csp6I GTAC 1 cut(s) 265
CviAII CATG 5 cut(s) 148, 286, 298, 319, 517
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 2 cut(s) 209, 249
DpnI GATC 3 cut(s) 52, 216, 693
DpnII GATC 3 cut(s) 50, 214, 691
Eam1104I CTCTTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 337
EcoO109I RGGNCCY 1 cut(s) 432
EcoRII CCWGG 3 cut(s) 304, 480, 606
FaeI CATG 5 cut(s) 151, 289, 301, 322, 520
FatI CATG 5 cut(s) 147, 285, 297, 318, 516
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 503, 636
FokI GGATG 2 cut(s) 53, 142
Fsp4HI GCNGC 2 cut(s) 503, 636
FspBI CTAG 2 cut(s) 662, 758
GlaI GCGC 1 cut(s) 501
GluI GCNGC 2 cut(s) 503, 636
GsaI CCCAGC 1 cut(s) 113
HaeIII GGCC 3 cut(s) 126, 433, 454
HapII CCGG 1 cut(s) 184
HhaI GCGC 1 cut(s) 502
Hin1II CATG 5 cut(s) 151, 289, 301, 322, 520
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HinfI GANTC 2 cut(s) 301, 348
HpaII CCGG 1 cut(s) 184
HphI GGTGA 3 cut(s) 209, 433, 564
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 5 cut(s) 160, 356, 530, 627, 671
Hpy188III TCNNGA 1 cut(s) 298
Hpy8I GTNNAC 1 cut(s) 139
HpyAV CCTTC 3 cut(s) 23, 29, 40
HpyCH4III ACNGT 5 cut(s) 91, 103, 269, 313, 649
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 460, 662
HpyF3I CTNAG 2 cut(s) 209, 249
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 5 cut(s) 151, 289, 301, 322, 520
HspAI GCGC 1 cut(s) 500
Kzo9I GATC 3 cut(s) 50, 214, 691
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 2 cut(s) 183, 468
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 480
MaeI CTAG 2 cut(s) 662, 758
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 236, 643, 745
MalI GATC 3 cut(s) 52, 216, 693
MboI GATC 3 cut(s) 50, 214, 691
MboII GAAGA 8 cut(s) 19, 236, 343, 390, 463, 507, 595, 718
MfeI CAATTG 2 cut(s) 576, 618
MflI RGATCY 2 cut(s) 50, 691
MluCI AATT 4 cut(s) 62, 576, 618, 674
MlyI GAGTC 1 cut(s) 357
MmeI TCCRAC 1 cut(s) 563
MnlI CCTC 7 cut(s) 19, 200, 209, 350, 423, 454, 625
MseI TTAA 2 cut(s) 533, 561
MslI CAYNNNNRTG 2 cut(s) 104, 758
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 4 cut(s) 184, 306, 482, 608
MunI CAATTG 2 cut(s) 576, 618
MvaI CCWGG 3 cut(s) 306, 482, 608
MvnI CGCG 1 cut(s) 502
MwoI GCNNNNNNNGC 2 cut(s) 460, 662
NciI CCSGG 1 cut(s) 184
NdeII GATC 3 cut(s) 50, 214, 691
NlaIII CATG 5 cut(s) 151, 289, 301, 322, 520
NlaIV GGNNCC 3 cut(s) 188, 337, 434
NmuCI GTSAC 2 cut(s) 236, 643
NspI RCATGY 1 cut(s) 520
OliI CACNNNNGTG 2 cut(s) 104, 758
PagI TCATGA 1 cut(s) 297
PciI ACATGT 1 cut(s) 516
PciSI GCTCTTC 1 cut(s) 490
PfeI GAWTC 1 cut(s) 301
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 2 cut(s) 504, 637
PleI GAGTC 1 cut(s) 356
PpsI GAGTC 1 cut(s) 356
PscI ACATGT 1 cut(s) 516
PshBI ATTAAT 1 cut(s) 561
Psp6I CCWGG 3 cut(s) 304, 480, 606
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 3 cut(s) 304, 480, 606
PspN4I GGNNCC 3 cut(s) 188, 337, 434
PspPI GGNCC 3 cut(s) 186, 432, 453
PsuI RGATCY 2 cut(s) 50, 691
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
RseI CAYNNNNRTG 2 cut(s) 104, 758
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 2 cut(s) 533, 561
SatI GCNGC 2 cut(s) 503, 636
Sau3AI GATC 3 cut(s) 50, 214, 691
Sau96I GGNCC 3 cut(s) 186, 432, 453
SchI GAGTC 1 cut(s) 357
ScrFI CCNGG 4 cut(s) 184, 306, 482, 608
SexAI ACCWGGT 1 cut(s) 480
SfaNI GCATC 1 cut(s) 164
SinI GGWCC 1 cut(s) 186
SmiMI CAYNNNNRTG 2 cut(s) 104, 758
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 4 cut(s) 62, 576, 618, 674
SsiI CCGC 2 cut(s) 153, 502
SspMI CTAG 2 cut(s) 662, 758
StyD4I CCNGG 4 cut(s) 182, 304, 480, 606
TaaI ACNGT 5 cut(s) 91, 103, 269, 313, 649
TaiI ACGT 1 cut(s) 645
TasI AATT 4 cut(s) 62, 576, 618, 674
TauI GCSGC 1 cut(s) 505
TfiI GAWTC 1 cut(s) 301
Tru1I TTAA 2 cut(s) 533, 561
Tru9I TTAA 2 cut(s) 533, 561
TscAI CASTG 2 cut(s) 391, 652
TseFI GTSAC 2 cut(s) 236, 643
TseI GCWGC 1 cut(s) 635
Tsp45I GTSAC 2 cut(s) 236, 643
TspDTI ATGAA 4 cut(s) 274, 286, 307, 718
TspRI CASTG 2 cut(s) 391, 652
VpaK11BI GGWCC 1 cut(s) 186
VspI ATTAAT 1 cut(s) 561
XapI RAATTY 1 cut(s) 62
XceI RCATGY 1 cut(s) 520
XcmI CCANNNNNNNNNTGG 2 cut(s) 75, 106
XspI CTAG 2 cut(s) 662, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.