Rw7G021520

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014316
Physical Location & Seq
Reverse (-)
24998559 .. 25001219
2661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021520.1

Sequence Viewer

Length: 771 bp
ATGGGGAAGATAGACAACAATGGGGAAGAGGGTGTAAAGGGATCTATCAGTAAATTCCCATTGAACTTTTGGGAGGTCACGGTGGCTTCCACGGTGGTGCTGGGTTTTCTGTTCGGCCTTCTGGGTGTTTACCTAACCATGCCCGCATCCGATTACAGCTTTCTCAAGCTCCCCCGGACCCTCCAAGACCTTCAAATCCTCAGAGATCACCTTGAGGACTACACAAGTGACTACACAGCTCAGGTTTTGTTAGGGTACTGTATGGTTTATATATTCATGCAGACATTCATGATTCCTGGAACTGTGTTCATGTCGTTGCTTGCTGGTTCCCTTTTCGGAGTCTTCAAAGGTGTAGCTTTGGTGGTGTTCAATGCCACTGCTGGTGCTTCTTCTTGCTATTTCTTGTCAAAGGTGATAGGGAGGCCCCTTGCCACTTCTCTTTGGCCCGACAAGCTCCTGTTTTTCCAAAACCAGGTGGCTAAAAGAAGACAAGGGTTGCTGAACTACATGTTGTTTCTCCGATTAACTCCAACCTTGCCAAACACATTTATTAATGTTGCTTCACCAATAGTCGATGTGCCTTATCATATTTTCTTCCTGGCAACCTCAATTGGTCTGATACCTGCTGCTTATGTCACTGTCAGGGCTGGACTAGCTCTTGGAGAATTGCGATCTCTTGGGGATCTCTATGACTTCAACTCAATAGCCACCTTGTTCTTCATCGGAGTTGTCTCAGTTACACCCACATTAGTGAGCAAGAACAAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

256

Amino Acids

28.18

Weight (kDa)

8.8

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 98 - 217 1.7e-14 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17790
fragaria_vesca FvH4_5g22470
malus_domestica MD06G1037100.v1.1 MD16G1272300.v1.1
prunus_persica Prupe.5G032200_v2.0.a1
pyrus_communis pycom16g24130
rosa_chinensis RchiOBHm_Chr7g0210261
rosa_laevigata RLG00000003054
rosa_multiflora Rmu_sc0017046.1_g000008
rosa_roxburghii Rroxscaffold_3G00248510
rosa_rugosa Rorug07G0120100
rosa_samantha Rh7AG253000 Rh7BG248100 Rh7CG270000 Rh7DG260600
rosa_wichuraiana Rw7G021520 Rw7G021570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 631
AciI CCGC 1 cut(s) 144
AclWI GGATC 2 cut(s) 49, 690
AcsI RAATTY 1 cut(s) 53
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 472
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 5 cut(s) 64, 194, 346, 370, 697
AjnI CCWGG 3 cut(s) 295, 471, 597
AleI CACNNNNGTG 2 cut(s) 95, 749
AluBI AGCT 6 cut(s) 159, 169, 239, 356, 454, 656
AluI AGCT 6 cut(s) 159, 169, 239, 356, 454, 656
Alw26I GTCTC 1 cut(s) 736
AlwI GGATC 2 cut(s) 49, 690
AoxI GGCC 3 cut(s) 115, 422, 443
ApeKI GCWGC 1 cut(s) 626
ApoI RAATTY 1 cut(s) 53
AseI ATTAAT 1 cut(s) 552
AspS9I GGNCC 3 cut(s) 177, 423, 444
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 3 cut(s) 200, 424, 555
AvaII GGWCC 1 cut(s) 177
BbsI GAAGAC 2 cut(s) 334, 493
BbvI GCAGC 1 cut(s) 613
BciT130I CCWGG 3 cut(s) 297, 473, 599
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 736
BfaI CTAG 1 cut(s) 653
BfuAI ACCTGC 1 cut(s) 631
BisI GCNGC 1 cut(s) 627
BlsI GCNGC 1 cut(s) 628
Bme1390I CCNGG 4 cut(s) 175, 297, 473, 599
Bme18I GGWCC 1 cut(s) 177
BmgT120I GGNCC 3 cut(s) 177, 423, 444
BmiI GGNNCC 3 cut(s) 179, 328, 425
BmrFI CCNGG 4 cut(s) 175, 297, 473, 599
BmsI GCATC 1 cut(s) 155
BpiI GAAGAC 2 cut(s) 334, 493
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 149, 233
BpuMI CCSGG 1 cut(s) 175
BsaJI CCNNGG 2 cut(s) 90, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 472
BseBI CCWGG 3 cut(s) 297, 473, 599
BseDI CCNNGG 2 cut(s) 90, 173
BseGI GGATG 1 cut(s) 146
BseLI CCNNNNNNNGG 1 cut(s) 472
BseMII CTCAG 3 cut(s) 214, 254, 747
BseXI GCAGC 1 cut(s) 613
BseYI CCCAGC 1 cut(s) 100
BshFI GGCC 3 cut(s) 117, 424, 445
BsiSI CCGG 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 472
BsmAI GTCTC 1 cut(s) 736
BsnI GGCC 3 cut(s) 117, 424, 445
Bsp143I GATC 4 cut(s) 41, 205, 671, 682
BspACI CCGC 1 cut(s) 144
BspANI GGCC 3 cut(s) 117, 424, 445
BspCNI CTCAG 3 cut(s) 213, 253, 746
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 3 cut(s) 179, 328, 425
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 2 cut(s) 49, 690
BssECI CCNNGG 2 cut(s) 90, 173
BssMI GATC 4 cut(s) 41, 205, 671, 682
Bst2UI CCWGG 3 cut(s) 297, 473, 599
Bst4CI ACNGT 5 cut(s) 82, 94, 260, 304, 640
Bst6I CTCTTC 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 144, 321
BstDEI CTNAG 3 cut(s) 200, 240, 733
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 1 cut(s) 146
BstKTI GATC 4 cut(s) 44, 208, 674, 685
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 4 cut(s) 41, 205, 671, 682
BstMWI GCNNNNNNNGC 2 cut(s) 451, 653
BstNI CCWGG 3 cut(s) 297, 473, 599
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 4 cut(s) 173, 295, 471, 597
BstV1I GCAGC 1 cut(s) 613
BstV2I GAAGAC 2 cut(s) 334, 493
BstX2I RGATCY 2 cut(s) 41, 682
BstYI RGATCY 2 cut(s) 41, 682
BsuRI GGCC 3 cut(s) 117, 424, 445
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 1 cut(s) 146
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 375, 636
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 2 cut(s) 144, 321
CciI TCATGA 1 cut(s) 288
Cfr13I GGNCC 3 cut(s) 177, 423, 444
CsiI ACCWGGT 1 cut(s) 471
Csp6I GTAC 1 cut(s) 256
CviAII CATG 5 cut(s) 139, 277, 289, 310, 508
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 3 cut(s) 200, 240, 733
DpnI GATC 4 cut(s) 43, 207, 673, 684
DpnII GATC 4 cut(s) 41, 205, 671, 682
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 423
EcoRII CCWGG 3 cut(s) 295, 471, 597
FaeI CATG 5 cut(s) 142, 280, 292, 313, 511
FatI CATG 5 cut(s) 138, 276, 288, 309, 507
FauI CCCGC 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 627
FokI GGATG 1 cut(s) 133
Fsp4HI GCNGC 1 cut(s) 627
FspBI CTAG 1 cut(s) 653
GluI GCNGC 1 cut(s) 627
GsaI CCCAGC 1 cut(s) 104
HaeIII GGCC 3 cut(s) 117, 424, 445
HapII CCGG 1 cut(s) 175
Hin1II CATG 5 cut(s) 142, 280, 292, 313, 511
HinfI GANTC 2 cut(s) 292, 339
HpaII CCGG 1 cut(s) 175
HphI GGTGA 3 cut(s) 200, 424, 555
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 6 cut(s) 151, 203, 338, 521, 618, 725
Hpy188III TCNNGA 1 cut(s) 289
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 2 cut(s) 128, 200
HpyCH4III ACNGT 5 cut(s) 82, 94, 260, 304, 640
HpyCH4V TGCA 1 cut(s) 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 451, 653
HpyF3I CTNAG 3 cut(s) 200, 240, 733
Hsp92II CATG 5 cut(s) 142, 280, 292, 313, 511
Kzo9I GATC 4 cut(s) 41, 205, 671, 682
LmnI GCTCC 2 cut(s) 174, 459
Lsp1109I GCAGC 1 cut(s) 613
LweI GCATC 1 cut(s) 155
MabI ACCWGGT 1 cut(s) 471
MaeI CTAG 1 cut(s) 653
MaeIII GTNAC 4 cut(s) 76, 227, 634, 736
MalI GATC 4 cut(s) 43, 207, 673, 684
MboI GATC 4 cut(s) 41, 205, 671, 682
MboII GAAGA 7 cut(s) 19, 38, 334, 381, 498, 586, 709
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 2 cut(s) 41, 682
MluCI AATT 3 cut(s) 53, 609, 665
MlyI GAGTC 1 cut(s) 348
MmeI TCCRAC 1 cut(s) 554
MnlI CCTC 7 cut(s) 22, 67, 191, 208, 209, 414, 616
MseI TTAA 2 cut(s) 524, 552
MslI CAYNNNNRTG 2 cut(s) 95, 749
MspI CCGG 1 cut(s) 175
MspR9I CCNGG 4 cut(s) 175, 297, 473, 599
MunI CAATTG 1 cut(s) 609
MvaI CCWGG 3 cut(s) 297, 473, 599
MwoI GCNNNNNNNGC 2 cut(s) 451, 653
NciI CCSGG 1 cut(s) 175
NdeII GATC 4 cut(s) 41, 205, 671, 682
NlaIII CATG 5 cut(s) 142, 280, 292, 313, 511
NlaIV GGNNCC 3 cut(s) 179, 328, 425
NmuCI GTSAC 3 cut(s) 76, 227, 634
NspI RCATGY 1 cut(s) 511
OliI CACNNNNGTG 2 cut(s) 95, 749
PagI TCATGA 1 cut(s) 288
PciI ACATGT 1 cut(s) 507
PfeI GAWTC 1 cut(s) 292
PfoI TCCNGGA 1 cut(s) 295
PkrI GCNGC 1 cut(s) 628
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
PscI ACATGT 1 cut(s) 507
PshBI ATTAAT 1 cut(s) 552
Psp6I CCWGG 3 cut(s) 295, 471, 597
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 3 cut(s) 295, 471, 597
PspN4I GGNNCC 3 cut(s) 179, 328, 425
PspPI GGNCC 3 cut(s) 177, 423, 444
PsuI RGATCY 2 cut(s) 41, 682
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 2 cut(s) 95, 749
SaqAI TTAA 2 cut(s) 524, 552
SatI GCNGC 1 cut(s) 627
Sau3AI GATC 4 cut(s) 41, 205, 671, 682
Sau96I GGNCC 3 cut(s) 177, 423, 444
SchI GAGTC 1 cut(s) 348
ScrFI CCNGG 4 cut(s) 175, 297, 473, 599
SexAI ACCWGGT 1 cut(s) 471
SfaNI GCATC 1 cut(s) 155
SinI GGWCC 1 cut(s) 177
SmiMI CAYNNNNRTG 2 cut(s) 95, 749
SmlI CTYRAG 2 cut(s) 164, 212
SmoI CTYRAG 2 cut(s) 164, 212
Sse9I AATT 3 cut(s) 53, 609, 665
SsiI CCGC 1 cut(s) 144
SspMI CTAG 1 cut(s) 653
StyD4I CCNGG 4 cut(s) 173, 295, 471, 597
TaaI ACNGT 5 cut(s) 82, 94, 260, 304, 640
TaqI TCGA 1 cut(s) 573
TasI AATT 3 cut(s) 53, 609, 665
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 2 cut(s) 524, 552
Tru9I TTAA 2 cut(s) 524, 552
TscAI CASTG 2 cut(s) 382, 643
TseFI GTSAC 3 cut(s) 76, 227, 634
TseI GCWGC 1 cut(s) 626
Tsp45I GTSAC 3 cut(s) 76, 227, 634
TspDTI ATGAA 4 cut(s) 265, 277, 298, 709
TspRI CASTG 2 cut(s) 382, 643
VpaK11BI GGWCC 1 cut(s) 177
VspI ATTAAT 1 cut(s) 552
XapI RAATTY 1 cut(s) 53
XceI RCATGY 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 2 cut(s) 66, 97
XspI CTAG 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.