MD06G1037100.v1.1

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014316
Physical Location & Seq
Reverse (-)
4710491 .. 4713008
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1037100.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGAGAAGAGAGACGACAATGGGGTGGACGTGAAGGTGGTTGGCCGGGTAGTGGGACCCAATAATATTAAGTTCCCGTTGAGCTTCTGGGAGGTGGCTCTGGCTTCCACCGTCGTATTGGGCTTCTTGTTTGGCCTTCTGGGTGTTTACCTGACCATGCCCGCATCGGATTACAGCTTCCTCAAGCTTCCCCGTAGCCTCCAAGAGCTTCAAATCCTCAGAGTTCACCTCGAGGACTACACAAGTGACTACACCGCGCAGGTTCTGTTAGGGTACTGCATGGTTTATATTTTCATGCAGACTTTCATGATCCCTGGTACCGTATTTATGTCCCTGCTTGCCGGTTCCCTTTTTGGAGTCTTCAAAGGTGTAGCCTTGGTGGTGTTTAACGCCACTGCTGGTGCATCTTCTTGTTATTTCTTGTCGAAGCTGATCGGGAGGCCCATTGTCATCTCTCTTTGGCCTGACAAGCTGCAATTTTTCCAAAACCAGGTAGCTAAAAGAAGGCAGGGACTATTGAACTATATGTTGTTTCTACGATTAACTCCGACCTTGCCAAATACATTTATTAATCTTGCTTCACCAATAGTTGATGTGCCTTACCATATTTTCTTACTGGCAACTTCAATTGGTCTGATACCAGCTGCTTACGTCACTGTCAGGGCTGGATTAGCTCTTGGAGAGTTACGATCCCTTGGGGATCTCTATGACTTCAACTCGATAGCCACCTTGTTCTTCATTGGAGTTGTCTCAATTACTCCCACGCTAGTGAGCAAGAACAAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

263

Amino Acids

28.94

Weight (kDa)

9.18

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 104 - 223 5.1e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17790
fragaria_vesca FvH4_5g22470
malus_domestica MD06G1037100.v1.1 MD16G1272300.v1.1
prunus_persica Prupe.5G032200_v2.0.a1
pyrus_communis pycom16g24130
rosa_chinensis RchiOBHm_Chr7g0210261
rosa_laevigata RLG00000003054
rosa_multiflora Rmu_sc0017046.1_g000008
rosa_roxburghii Rroxscaffold_3G00248510
rosa_rugosa Rorug07G0120100
rosa_samantha Rh7AG253000 Rh7BG248100 Rh7CG270000 Rh7DG260600
rosa_wichuraiana Rw7G021520 Rw7G021570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 230
Acc36I ACCTGC 1 cut(s) 250
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 1 cut(s) 317
AccII CGCG 1 cut(s) 257
AciI CCGC 2 cut(s) 162, 255
AclWI GGATC 3 cut(s) 304, 684, 708
AcoI YGGCCR 1 cut(s) 43
AfaI GTAC 2 cut(s) 275, 319
AfiI CCNNNNNNNGG 2 cut(s) 52, 490
AgsI TTSAA 5 cut(s) 212, 364, 520, 627, 715
AjiI CACGTC 1 cut(s) 31
AjnI CCWGG 2 cut(s) 313, 489
AleI CACNNNNGTG 1 cut(s) 767
AluBI AGCT 9 cut(s) 84, 177, 187, 208, 430, 472, 497, 644, 674
AluI AGCT 9 cut(s) 84, 177, 187, 208, 430, 472, 497, 644, 674
Alw26I GTCTC 2 cut(s) 6, 754
AlwI GGATC 3 cut(s) 304, 684, 708
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 4 cut(s) 43, 133, 440, 461
ApeKI GCWGC 2 cut(s) 472, 644
AseI ATTAAT 1 cut(s) 570
Asp718I GGTACC 1 cut(s) 317
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 2 cut(s) 56, 441
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 218, 573
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 352
BbvI GCAGC 2 cut(s) 459, 631
BciT130I CCWGG 2 cut(s) 315, 491
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 2 cut(s) 6, 754
BfaI CTAG 1 cut(s) 767
BfuAI ACCTGC 1 cut(s) 250
BisI GCNGC 2 cut(s) 473, 645
BlsI GCNGC 2 cut(s) 474, 646
Bme1390I CCNGG 3 cut(s) 47, 315, 491
Bme18I GGWCC 1 cut(s) 56
BmeT110I CYCGRG 1 cut(s) 230
BmgBI CACGTC 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 56, 441
BmiI GGNNCC 4 cut(s) 57, 58, 319, 346
BmrFI CCNGG 3 cut(s) 47, 315, 491
BmsI GCATC 2 cut(s) 173, 413
BpiI GAAGAC 1 cut(s) 352
BplI GAGNNNNNCTC 2 cut(s) 213, 245
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 47
BsaJI CCNNGG 3 cut(s) 313, 375, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 490
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 621
BseBI CCWGG 2 cut(s) 315, 491
BseDI CCNNGG 3 cut(s) 313, 375, 694
BseLI CCNNNNNNNGG 2 cut(s) 52, 490
BseMII CTCAG 1 cut(s) 232
BseNI ACTGG 1 cut(s) 621
BseXI GCAGC 2 cut(s) 459, 631
Bsh1236I CGCG 1 cut(s) 257
BshFI GGCC 4 cut(s) 45, 135, 442, 463
BshNI GGYRCC 1 cut(s) 317
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 2 cut(s) 46, 342
BslFI GGGAC 3 cut(s) 69, 316, 525
BslI CCNNNNNNNGG 2 cut(s) 52, 490
BsmAI GTCTC 2 cut(s) 6, 754
BsmBI CGTCTC 1 cut(s) 6
BsmFI GGGAC 3 cut(s) 69, 316, 525
BsnI GGCC 4 cut(s) 45, 135, 442, 463
BsoBI CYCGRG 1 cut(s) 230
Bsp143I GATC 4 cut(s) 309, 432, 689, 700
BspACI CCGC 2 cut(s) 162, 255
BspANI GGCC 4 cut(s) 45, 135, 442, 463
BspCNI CTCAG 1 cut(s) 231
BspFNI CGCG 1 cut(s) 257
BspHI TCATGA 1 cut(s) 306
BspLI GGNNCC 4 cut(s) 57, 58, 319, 346
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 3 cut(s) 304, 684, 708
BspT107I GGYRCC 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 621
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 3 cut(s) 313, 375, 694
BssMI GATC 4 cut(s) 309, 432, 689, 700
BssT1I CCWWGG 2 cut(s) 375, 694
Bst2UI CCWGG 2 cut(s) 315, 491
Bst4CI ACNGT 3 cut(s) 112, 322, 658
Bst6I CTCTTC 1 cut(s) 2
BstC8I GCNNGC 2 cut(s) 162, 339
BstDEI CTNAG 1 cut(s) 218
BstFNI CGCG 1 cut(s) 257
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 4 cut(s) 312, 435, 692, 703
BstMAI GTCTC 2 cut(s) 6, 754
BstMBI GATC 4 cut(s) 309, 432, 689, 700
BstMWI GCNNNNNNNGC 2 cut(s) 469, 671
BstNI CCWGG 2 cut(s) 315, 491
BstSCI CCNGG 3 cut(s) 45, 313, 489
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 2 cut(s) 459, 631
BstV2I GAAGAC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 700
BstYI RGATCY 1 cut(s) 700
BsuRI GGCC 4 cut(s) 45, 135, 442, 463
BtrI CACGTC 1 cut(s) 31
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 2 cut(s) 393, 654
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 2 cut(s) 162, 339
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 306
CfoI GCGC 1 cut(s) 259
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 2 cut(s) 56, 441
CsiI ACCWGGT 1 cut(s) 489
Csp6I GTAC 2 cut(s) 274, 318
CviAII CATG 4 cut(s) 157, 280, 295, 307
CviQI GTAC 2 cut(s) 274, 318
DdeI CTNAG 1 cut(s) 218
DpnI GATC 4 cut(s) 311, 434, 691, 702
DpnII GATC 4 cut(s) 309, 432, 689, 700
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
Eco130I CCWWGG 2 cut(s) 375, 694
Eco47I GGWCC 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 2 cut(s) 313, 489
EcoT14I CCWWGG 2 cut(s) 375, 694
ErhI CCWWGG 2 cut(s) 375, 694
Esp3I CGTCTC 1 cut(s) 6
FaeI CATG 4 cut(s) 160, 283, 298, 310
FaqI GGGAC 3 cut(s) 69, 316, 525
FatI CATG 4 cut(s) 156, 279, 294, 306
FauI CCCGC 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 473, 645
Fsp4HI GCNGC 2 cut(s) 473, 645
FspBI CTAG 1 cut(s) 767
GlaI GCGC 1 cut(s) 258
GluI GCNGC 2 cut(s) 473, 645
HaeIII GGCC 4 cut(s) 45, 135, 442, 463
HapII CCGG 2 cut(s) 46, 342
HhaI GCGC 1 cut(s) 259
Hin1II CATG 4 cut(s) 160, 283, 298, 310
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 1 cut(s) 357
HpaII CCGG 2 cut(s) 46, 342
HphI GGTGA 2 cut(s) 218, 573
Hpy166II GTNNAC 3 cut(s) 28, 148, 226
Hpy188I TCNGA 4 cut(s) 169, 221, 549, 636
Hpy188III TCNNGA 2 cut(s) 307, 436
Hpy8I GTNNAC 3 cut(s) 28, 148, 226
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 3 cut(s) 28, 146, 498
HpyCH4III ACNGT 3 cut(s) 112, 322, 658
HpyCH4IV ACGT 2 cut(s) 30, 651
HpyCH4V TGCA 4 cut(s) 279, 298, 404, 475
HpyF10VI GCNNNNNNNGC 2 cut(s) 469, 671
HpyF3I CTNAG 1 cut(s) 218
HpySE526I ACGT 2 cut(s) 30, 651
Hsp92II CATG 4 cut(s) 160, 283, 298, 310
HspAI GCGC 1 cut(s) 257
KflI GGGWCCC 1 cut(s) 56
KpnI GGTACC 1 cut(s) 321
Kzo9I GATC 4 cut(s) 309, 432, 689, 700
Lsp1109I GCAGC 2 cut(s) 459, 631
LweI GCATC 2 cut(s) 173, 413
MabI ACCWGGT 1 cut(s) 489
MaeI CTAG 1 cut(s) 767
MaeII ACGT 2 cut(s) 30, 651
MaeIII GTNAC 3 cut(s) 245, 652, 684
MalI GATC 4 cut(s) 311, 434, 691, 702
MboI GATC 4 cut(s) 309, 432, 689, 700
MboII GAAGA 4 cut(s) 19, 352, 399, 727
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 1 cut(s) 700
MluCI AATT 3 cut(s) 476, 627, 753
MlyI GAGTC 1 cut(s) 366
MmeI TCCRAC 1 cut(s) 572
MnlI CCTC 7 cut(s) 85, 191, 209, 226, 227, 239, 432
MseI TTAA 4 cut(s) 69, 387, 542, 570
MslI CAYNNNNRTG 1 cut(s) 767
MspA1I CMGCKG 1 cut(s) 644
MspI CCGG 2 cut(s) 46, 342
MspR9I CCNGG 3 cut(s) 47, 315, 491
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 2 cut(s) 315, 491
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 2 cut(s) 469, 671
NciI CCSGG 1 cut(s) 47
NdeII GATC 4 cut(s) 309, 432, 689, 700
NlaIII CATG 4 cut(s) 160, 283, 298, 310
NlaIV GGNNCC 4 cut(s) 57, 58, 319, 346
NmuCI GTSAC 2 cut(s) 245, 652
OliI CACNNNNGTG 1 cut(s) 767
PaeR7I CTCGAG 1 cut(s) 230
PagI TCATGA 1 cut(s) 306
PkrI GCNGC 2 cut(s) 474, 646
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 570
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 313, 489
PspGI CCWGG 2 cut(s) 313, 489
PspN4I GGNNCC 4 cut(s) 57, 58, 319, 346
PspPI GGNCC 2 cut(s) 56, 441
PspPPI RGGWCCY 1 cut(s) 56
PspXI VCTCGAGB 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 700
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 275, 319
RsaNI GTAC 2 cut(s) 274, 318
RseI CAYNNNNRTG 1 cut(s) 767
SaqAI TTAA 4 cut(s) 69, 387, 542, 570
SatI GCNGC 2 cut(s) 473, 645
Sau3AI GATC 4 cut(s) 309, 432, 689, 700
Sau96I GGNCC 2 cut(s) 56, 441
SchI GAGTC 1 cut(s) 366
ScrFI CCNGG 3 cut(s) 47, 315, 491
SexAI ACCWGGT 1 cut(s) 489
SfaNI GCATC 2 cut(s) 173, 413
Sfr274I CTCGAG 1 cut(s) 230
SinI GGWCC 1 cut(s) 56
SlaI CTCGAG 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 767
SmlI CTYRAG 2 cut(s) 182, 230
SmoI CTYRAG 2 cut(s) 182, 230
Sse9I AATT 3 cut(s) 476, 627, 753
SsiI CCGC 2 cut(s) 162, 255
SspI AATATT 1 cut(s) 67
SspMI CTAG 1 cut(s) 767
StyD4I CCNGG 3 cut(s) 45, 313, 489
StyI CCWWGG 2 cut(s) 375, 694
TaaI ACNGT 3 cut(s) 112, 322, 658
TaiI ACGT 2 cut(s) 33, 654
TaqI TCGA 3 cut(s) 231, 425, 719
TasI AATT 3 cut(s) 476, 627, 753
Tru1I TTAA 4 cut(s) 69, 387, 542, 570
Tru9I TTAA 4 cut(s) 69, 387, 542, 570
TscAI CASTG 2 cut(s) 400, 661
TseFI GTSAC 2 cut(s) 245, 652
TseI GCWGC 2 cut(s) 472, 644
Tsp45I GTSAC 2 cut(s) 245, 652
TspDTI ATGAA 3 cut(s) 283, 295, 727
TspRI CASTG 2 cut(s) 400, 661
VpaK11BI GGWCC 1 cut(s) 56
VspI ATTAAT 1 cut(s) 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XhoI CTCGAG 1 cut(s) 230
XspI CTAG 1 cut(s) 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.