MD16G1272300.v1.1

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014316
Physical Location & Seq
Forward (+)
35448118 .. 35451089
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1272300.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGGGAAAGGAGACGACAGTGGCGGCGATGTGAAGGTGGTTGGTGCAGAAGTGGGATCCGATAATAGTAAGTTCCCGTTGAGCTTTTGGGAGGTGACCCTGGCTTCCACCGTCGTATTGGGCTTCTTGTTTGGCCTTCTGGCTGTTTACCTGACCATGCCCGCATCGGATTACAGCTTCCTTAAGCTTCCCCGTAGCCTCCAAGATCTTCGAATCCTCAGAGTTCACCTCGAGGACTACACAAGTGACTACACTGCTCAGGTTCTGTTAGGATACTGCATGGTTTATATATTCATGCAGACTTTCATGATCCCTGGTACTGTATTTATGTCCTTGCTTGCCGGTTCCCTTTTTGGAGTCTTCAAAGGTGTAGCTTTGGTGGTGTTTAACGCTACTGCTGGTGCATCTTCTTGTTTTTTCTTGTCAAAGCTGATCGGGAGACCTCTTGTCTTCTCTCTTTGGCCCGACAAGCTGCAATTTTTCCAAAACCAGGTAGCTAAAAGAAGAGAGGGACTATTGAACTATATGCTGTTTCTACGGTTAACTCCGACCTTGCCAAATACATTTATTAATGTTGCTTCACCAATAGTTGATGTGCCTTACCGTATATTCTTCCTGGCAACTTCAATTGGTCTGATACCGGCTGCTTACGTCACTGTCAGGGCTGGATTAGTTCTTGGAGAGTTGCGATCCCTTGGGGATCTCTATGACTTCAACTCGATAGCCACCTTGTTCTTCATTGGAGTTGTCTCAATTACTCCCACGCTAGTGAGCAAGAGCAAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

263

Amino Acids

28.75

Weight (kDa)

8.81

Isoelectric Point (pI)

31.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 104 - 223 2.7e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17790
fragaria_vesca FvH4_5g22470
malus_domestica MD06G1037100.v1.1 MD16G1272300.v1.1
prunus_persica Prupe.5G032200_v2.0.a1
pyrus_communis pycom16g24130
rosa_chinensis RchiOBHm_Chr7g0210261
rosa_laevigata RLG00000003054
rosa_multiflora Rmu_sc0017046.1_g000008
rosa_roxburghii Rroxscaffold_3G00248510
rosa_rugosa Rorug07G0120100
rosa_samantha Rh7AG253000 Rh7BG248100 Rh7CG270000 Rh7DG260600
rosa_wichuraiana Rw7G021520 Rw7G021570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 230
AciI CCGC 2 cut(s) 24, 162
AclWI GGATC 5 cut(s) 51, 64, 304, 684, 708
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 1 cut(s) 490
AflII CTTAAG 1 cut(s) 182
AgsI TTSAA 4 cut(s) 364, 520, 627, 715
AhdI GACNNNNNGTC 1 cut(s) 446
AjnI CCWGG 4 cut(s) 99, 313, 489, 615
AleI CACNNNNGTG 1 cut(s) 767
AluBI AGCT 7 cut(s) 84, 177, 187, 374, 430, 472, 497
AluI AGCT 7 cut(s) 84, 177, 187, 374, 430, 472, 497
Alw26I GTCTC 3 cut(s) 6, 433, 754
AlwI GGATC 5 cut(s) 51, 64, 304, 684, 708
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 2 cut(s) 133, 461
ApeKI GCWGC 2 cut(s) 472, 644
AseI ATTAAT 1 cut(s) 570
AspS9I GGNCC 1 cut(s) 462
AsuHPI GGTGA 3 cut(s) 106, 218, 573
AsuII TTCGAA 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 230
BamHI GGATCC 1 cut(s) 56
BbsI GAAGAC 2 cut(s) 352, 442
BbvI GCAGC 2 cut(s) 459, 631
BciT130I CCWGG 4 cut(s) 101, 315, 491, 617
BciVI GTATCC 1 cut(s) 266
BcoDI GTCTC 3 cut(s) 6, 433, 754
BfaI CTAG 1 cut(s) 767
BfrI CTTAAG 1 cut(s) 182
BfuI GTATCC 1 cut(s) 266
BglII AGATCT 1 cut(s) 205
BisI GCNGC 3 cut(s) 25, 473, 645
BlsI GCNGC 3 cut(s) 26, 474, 646
Bme1390I CCNGG 4 cut(s) 101, 315, 491, 617
BmeRI GACNNNNNGTC 1 cut(s) 446
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 462
BmiI GGNNCC 2 cut(s) 58, 346
BmrFI CCNGG 4 cut(s) 101, 315, 491, 617
BmsI GCATC 2 cut(s) 173, 413
BpiI GAAGAC 2 cut(s) 352, 442
BplI GAGNNNNNCTC 2 cut(s) 213, 245
Bpu10I CCTNAGC 1 cut(s) 258
Bpu14I TTCGAA 1 cut(s) 211
BsaI GGTCTC 1 cut(s) 433
BsaJI CCNNGG 3 cut(s) 99, 313, 694
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse118I RCCGGY 2 cut(s) 341, 640
BseBI CCWGG 4 cut(s) 101, 315, 491, 617
BseDI CCNNGG 3 cut(s) 99, 313, 694
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMII CTCAG 2 cut(s) 232, 272
BseXI GCAGC 2 cut(s) 459, 631
BsgI GTGCAG 1 cut(s) 66
BshFI GGCC 2 cut(s) 135, 463
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 2 cut(s) 342, 641
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 3 cut(s) 6, 433, 754
BsmBI CGTCTC 1 cut(s) 6
BsmFI GGGAC 1 cut(s) 525
BsnI GGCC 2 cut(s) 135, 463
Bso31I GGTCTC 1 cut(s) 433
BsoBI CYCGRG 1 cut(s) 230
Bsp119I TTCGAA 1 cut(s) 211
Bsp143I GATC 6 cut(s) 56, 205, 309, 432, 689, 700
BspACI CCGC 2 cut(s) 24, 162
BspANI GGCC 2 cut(s) 135, 463
BspCNI CTCAG 2 cut(s) 231, 271
BspHI TCATGA 1 cut(s) 306
BspLI GGNNCC 2 cut(s) 58, 346
BspPI GGATC 5 cut(s) 51, 64, 304, 684, 708
BspT104I TTCGAA 1 cut(s) 211
BspTI CTTAAG 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 433
BsrFI RCCGGY 2 cut(s) 341, 640
BssAI RCCGGY 2 cut(s) 341, 640
BssECI CCNNGG 3 cut(s) 99, 313, 694
BssMI GATC 6 cut(s) 56, 205, 309, 432, 689, 700
BssT1I CCWWGG 1 cut(s) 694
Bst2UI CCWGG 4 cut(s) 101, 315, 491, 617
Bst4CI ACNGT 6 cut(s) 20, 112, 322, 540, 605, 658
Bst6I CTCTTC 1 cut(s) 499
BstAFI CTTAAG 1 cut(s) 182
BstBI TTCGAA 1 cut(s) 211
BstC8I GCNNGC 2 cut(s) 162, 339
BstDEI CTNAG 2 cut(s) 218, 258
BstEII GGTNACC 1 cut(s) 94
BstKTI GATC 6 cut(s) 59, 208, 312, 435, 692, 703
BstMAI GTCTC 3 cut(s) 6, 433, 754
BstMBI GATC 6 cut(s) 56, 205, 309, 432, 689, 700
BstMWI GCNNNNNNNGC 1 cut(s) 469
BstNI CCWGG 4 cut(s) 101, 315, 491, 617
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 4 cut(s) 99, 313, 489, 615
BstV1I GCAGC 2 cut(s) 459, 631
BstV2I GAAGAC 2 cut(s) 352, 442
BstX2I RGATCY 3 cut(s) 56, 205, 700
BstYI RGATCY 3 cut(s) 56, 205, 700
BsuI GTATCC 1 cut(s) 266
BsuRI GGCC 2 cut(s) 135, 463
BtgZI GCGATG 1 cut(s) 42
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 3 cut(s) 25, 252, 654
Cac8I GCNNGC 2 cut(s) 162, 339
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 306
Cfr10I RCCGGY 2 cut(s) 341, 640
Cfr13I GGNCC 1 cut(s) 462
CsiI ACCWGGT 1 cut(s) 489
Csp6I GTAC 1 cut(s) 318
CviAII CATG 4 cut(s) 157, 280, 295, 307
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 2 cut(s) 218, 258
DpnI GATC 6 cut(s) 58, 207, 311, 434, 691, 702
DpnII GATC 6 cut(s) 56, 205, 309, 432, 689, 700
DriI GACNNNNNGTC 1 cut(s) 446
Eam1104I CTCTTC 1 cut(s) 499
Eam1105I GACNNNNNGTC 1 cut(s) 446
EarI CTCTTC 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 694
Eco31I GGTCTC 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 230
Eco91I GGTNACC 1 cut(s) 94
EcoO65I GGTNACC 1 cut(s) 94
EcoRII CCWGG 4 cut(s) 99, 313, 489, 615
EcoT14I CCWWGG 1 cut(s) 694
ErhI CCWWGG 1 cut(s) 694
Esp3I CGTCTC 1 cut(s) 6
FaeI CATG 4 cut(s) 160, 283, 298, 310
FaqI GGGAC 1 cut(s) 525
FatI CATG 4 cut(s) 156, 279, 294, 306
FauI CCCGC 1 cut(s) 169
Fnu4HI GCNGC 3 cut(s) 25, 473, 645
Fsp4HI GCNGC 3 cut(s) 25, 473, 645
FspBI CTAG 1 cut(s) 767
GluI GCNGC 3 cut(s) 25, 473, 645
HaeIII GGCC 2 cut(s) 135, 463
HapII CCGG 2 cut(s) 342, 641
Hin1II CATG 4 cut(s) 160, 283, 298, 310
HincII GTYRAC 1 cut(s) 543
HindII GTYRAC 1 cut(s) 543
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 2 cut(s) 213, 357
HpaI GTTAAC 1 cut(s) 543
HpaII CCGG 2 cut(s) 342, 641
HphI GGTGA 3 cut(s) 106, 218, 573
Hpy166II GTNNAC 3 cut(s) 148, 226, 543
Hpy188I TCNGA 5 cut(s) 61, 169, 221, 549, 636
Hpy188III TCNNGA 2 cut(s) 307, 436
Hpy8I GTNNAC 3 cut(s) 148, 226, 543
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 2 cut(s) 28, 146
HpyCH4III ACNGT 6 cut(s) 20, 112, 322, 540, 605, 658
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 5 cut(s) 47, 279, 298, 404, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 469
HpyF3I CTNAG 2 cut(s) 218, 258
HpySE526I ACGT 1 cut(s) 651
Hsp92II CATG 4 cut(s) 160, 283, 298, 310
KspAI GTTAAC 1 cut(s) 543
Kzo9I GATC 6 cut(s) 56, 205, 309, 432, 689, 700
Lsp1109I GCAGC 2 cut(s) 459, 631
LweI GCATC 2 cut(s) 173, 413
MabI ACCWGGT 1 cut(s) 489
MaeI CTAG 1 cut(s) 767
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 3 cut(s) 94, 245, 652
MalI GATC 6 cut(s) 58, 207, 311, 434, 691, 702
MboI GATC 6 cut(s) 56, 205, 309, 432, 689, 700
MboII GAAGA 7 cut(s) 200, 352, 399, 442, 516, 604, 727
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 3 cut(s) 56, 205, 700
MluCI AATT 3 cut(s) 476, 627, 753
MlyI GAGTC 1 cut(s) 366
MmeI TCCRAC 1 cut(s) 572
MnlI CCTC 7 cut(s) 85, 209, 226, 227, 239, 453, 502
MseI TTAA 4 cut(s) 183, 387, 542, 570
MslI CAYNNNNRTG 1 cut(s) 767
MspCI CTTAAG 1 cut(s) 182
MspI CCGG 2 cut(s) 342, 641
MspR9I CCNGG 4 cut(s) 101, 315, 491, 617
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 4 cut(s) 101, 315, 491, 617
MwoI GCNNNNNNNGC 1 cut(s) 469
NdeII GATC 6 cut(s) 56, 205, 309, 432, 689, 700
NlaIII CATG 4 cut(s) 160, 283, 298, 310
NlaIV GGNNCC 2 cut(s) 58, 346
NmuCI GTSAC 3 cut(s) 94, 245, 652
NspV TTCGAA 1 cut(s) 211
OliI CACNNNNGTG 1 cut(s) 767
PaeR7I CTCGAG 1 cut(s) 230
PagI TCATGA 1 cut(s) 306
PfeI GAWTC 1 cut(s) 213
PkrI GCNGC 3 cut(s) 26, 474, 646
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
PshBI ATTAAT 1 cut(s) 570
Psp6I CCWGG 4 cut(s) 99, 313, 489, 615
PspEI GGTNACC 1 cut(s) 94
PspGI CCWGG 4 cut(s) 99, 313, 489, 615
PspN4I GGNNCC 2 cut(s) 58, 346
PspPI GGNCC 1 cut(s) 462
PspXI VCTCGAGB 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 3 cut(s) 56, 205, 700
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
RseI CAYNNNNRTG 1 cut(s) 767
SaqAI TTAA 4 cut(s) 183, 387, 542, 570
SatI GCNGC 3 cut(s) 25, 473, 645
Sau3AI GATC 6 cut(s) 56, 205, 309, 432, 689, 700
Sau96I GGNCC 1 cut(s) 462
SchI GAGTC 1 cut(s) 366
ScrFI CCNGG 4 cut(s) 101, 315, 491, 617
SexAI ACCWGGT 1 cut(s) 489
SfaNI GCATC 2 cut(s) 173, 413
Sfr274I CTCGAG 1 cut(s) 230
SfuI TTCGAA 1 cut(s) 211
SlaI CTCGAG 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 767
SmlI CTYRAG 2 cut(s) 182, 230
SmoI CTYRAG 2 cut(s) 182, 230
Sse9I AATT 3 cut(s) 476, 627, 753
SsiI CCGC 2 cut(s) 24, 162
SspMI CTAG 1 cut(s) 767
StyD4I CCNGG 4 cut(s) 99, 313, 489, 615
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 6 cut(s) 20, 112, 322, 540, 605, 658
TaiI ACGT 1 cut(s) 654
TaqI TCGA 3 cut(s) 211, 231, 719
TasI AATT 3 cut(s) 476, 627, 753
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 1 cut(s) 213
Tru1I TTAA 4 cut(s) 183, 387, 542, 570
Tru9I TTAA 4 cut(s) 183, 387, 542, 570
TscAI CASTG 3 cut(s) 25, 259, 661
TseFI GTSAC 3 cut(s) 94, 245, 652
TseI GCWGC 2 cut(s) 472, 644
Tsp45I GTSAC 3 cut(s) 94, 245, 652
TspDTI ATGAA 3 cut(s) 283, 295, 727
TspRI CASTG 3 cut(s) 25, 259, 661
Vha464I CTTAAG 1 cut(s) 182
VspI ATTAAT 1 cut(s) 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XhoI CTCGAG 1 cut(s) 230
XspI CTAG 1 cut(s) 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.