FvH4_5g28640

leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010572
Physical Location & Seq
Reverse (-)
19816073 .. 19820411
4339 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28640.t1

Sequence Viewer

Length: 3036 bp
ATGGTGGTGCCTTTGATTTTCTTGTCACTTTTCCCTGTTCTAGGATCAATAATCCCCTGTTTTGCTTCTTCTTTGGTCAGTGATTTTCATGTCTTGCTCACACTCAAGCAAGGCTTCAAATTCTCCGAACCCGCTTTAAGTAGTTGGAATTCTTCGTCGTCTCCGACGTCAGTTTGTTCCAGCTGGGTTGGGATTCGATGCTCTAGAGGACGTGTTGTTGCTGTGGACTTGACAGATTTGAGTCTCTCTGGTTCTGTTTCACCTATCATTTCAGGCCTTGACCGGCTTTCCGACCTATCTCTTGCCGGAAACAACTTGTCAGGAAGCATTGAGCTTGCAAACTTGGGCAGCCTACGATCCCTCAACATCTCCGACAACCAGTTCAGTGGGGGCTTAGACTGGAACTTCTCCAGCATTGCCAACTTGGAGGTTTTTGATGTTTATGATAACAACTTGACCGCTCCACTTCCACTTGGGATTTTGAACTTGAGTAAGCTCAGGTACTTGGATCTCGGAGGCAACTTCTTCTATGGGAAAGTCCCACCATTGTACGGAAATCTTGTCAGCTTGGAGTACTTGTCACTCTCAGGCAATGATCTTCACGGTGAAATTCCCGGTGAGTTAGGCAATCTCACTAACCTGAGAGAGATCTACTTGGGCTACTACAATGTCTTTGAAGGTGGGATTCCCAAGGAGTTTGGGAAACTGGTGAATCTTGTTCACATGGATCTTTCAAGTTGTGAACTGGATGGACAGATCCCTCATGAGTTGGGGAATTTGAAGTCCCTTGACACGCTTTACCTGCATATCAATCTGCTCTCAGGCTCAATTCCAAGGCAGTTGGGCAACTTGACAAGCTTGCTCTATTTCGACCTCTCCAACAATGCACTTACTGGTGAAATTCCATCCGAGTTTTCAAATCTCAGACAGCTCAAGCTCTTTAACCTCTTCATGAACCGATTGCATGGGTCTATTCCAGACTACGTTGCGGATTTGCCTGATTTGGACACTCTGGGGCTGTGGATGAACAACTTCACAGGCATTATTCCTCAGAAACTCGGCCAAAATAGGAAGCTGCAGCTGCTGGATTTGTCCTCAAATAAGCTCACCGGTAAAATACCCCCAAACTTGTGTTCATCCAATCAGCTAAGGATACTAATTCTGTTGAAAAACTTTCTGTTTGGACCAATCCCTTCAGGCTTGGGGACTTGTTGTAGTCTTACTAGACTCAGACTGGGCCAGAATTACCTGAATGGTAGCATTCCAAATGGCTTGATATACTTGCCTCTGCTCAGTTTAGCAGAGTTGCAAGATAATTATCTCTCTGGCACGTTGTCGGAGAATGCCAATAGTTCATCACAGCCTGCACTATTAGGCCAGCTTATTCTCTCAAACAACCTCTTGTCCGGTAAAATACCATATTCCATTTCAAATTTCTCTTCCCTCCAAATCTTCTCACTCAGTGGAAATCAGCTCTCTGGTCCAATCCCACCATCTATAGGCCAACTCCATCAAGTACTAAACCTTGATCTTAGCCGGAATTCACTCTCGGGTGAAATTCCTCCGGAAATTGGAAACTGTTTCCATCTTACTTACCTTGACATGAGTCAGAACAACCTCTCTGGCTCAATCCCATCCGAAATCTCCAGCATTCATATACTGAATTACATCAACATGTCAAGAAACCACTTGAACCAGAGCATACCAAAATCCTTGGCAACCATGAAGAGCCTCACAACTGCGGATTTTTCCTTCAATGACTTGTCGGGTAAGCTACCCGAAGCGGGGCAGTTTGCCTTCTTCAACGCTTCCTCTTTCGCTGGGAATCCCCAGCTTTGCGGTTCCCTCCTAAACAATCCTTGCAATTTCACCAGCATCACAGACTCACCCGGAAATCCTCGGGCAGATTTCAAGCTAATATTCGCATTAGGCTTGCTCATATGCTCGCTAGTGTTTGCAGCTGCAGCCATAATGAAGGCGAAATCGTTCAAAAGAAAAGGCCCGGAATCATGGAAAATGACAGCATTTCAGAAGGTGGAGTTCACAATGTCGGACGTGCTGGAATGCGTCAAGGATGGAAATGTGATCGGGAGAGGAGGAGCAGGAATTGTTTACCACGGGAAAATGCCAAACGGGGTTGAAATTGCAGTGAAAAAGCTTCTTGGTTTTGGTTTTGGTCTAGCTCCCAACAGCACCCATGACGACCATGGATTCAGAGCCGAAATCCAAACATTGGGCAACATTCGTCACAGAAACATCGTGAGATTGCTGGCATTTTGCTCAAACAAGGAGACCAATCTACTTGTTTACGAGTACATGAGGAACGGAAGTTTAGGAGAGGCATTGCATGTCAACAATAAAAAAGGTGGCCTATTCTTGAGTTGGAATTTGAGGTACAAAATTGCAATTGAAGCTGCCAAAGGCTTGTGCTATCTCCACCATGACTGCTCCCCATTGATCCTTCATCGAGACGTCAAATCCAACAACATTTTGCTCAACTCCAGCTTTGAAGCACACGTGGCTGATTTCGGCCTCGCCAAGTTCTTGATCGACGGCGGCGGAGCATCCCAGTGCATGTCAGCAATTGCAGGCTCTTACGGCTACATTGCGCCTGAGTATGCATACACACTAAGGGTGGATGAGAAAAGCGACGTATATAGCTTTGGTGTTGTACTGTTAGAGCTTCTAACAGGGCGTCGGCCGGTGGGTGAGTTCGGCGAAGGCATCGACATAGTTCAATGGTCTAAAAAGGTGACCAATTGTCGAAAACAAGACGCTGCAGACATTGTTGATCCGAGGCTCGCCATTTCTGTGCCCAAAGATGAAGCAATGCACTTGTTCTTCATTGCAATGCATTGTGTACAAGAAAACAGTGTTGAGAGGCCAACAATGAGAGAAGTTGTTCAAATGTTGTCAGAATTTCCTCGCGCCCATTCTCCCGACTCCTCCTCTTCTTTAATCCCTTCCCAACAATTCGATAACAAACAAAATGACAGTAAATGTCCTCCTAAATTTAAACAACATATTTTGGTTTAA

Protein Analysis

1012

Amino Acids

110.43

Weight (kDa)

6.46

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 68 2.5e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 99 - 219 1.3e-08 Leucine-rich repeat region
LRR_14 PF23598 218 - 319 1.3e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 693 - 971 1.1e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 693 - 969 2e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 170, 2475
Acc36I ACCTGC 1 cut(s) 810
AccB1I GGYRCC 1 cut(s) 7
AccB7I CCANNNNNTGG 1 cut(s) 2233
AccBSI CCGCTC 1 cut(s) 461
AccII CGCG 1 cut(s) 2928
AccIII TCCGGA 1 cut(s) 1564
AciI CCGC 8 cut(s) 132, 459, 989, 1742, 1784, 1839, 2556, 2559
AclWI GGATC 7 cut(s) 52, 351, 516, 735, 751, 2452, 2786
AcoI YGGCCR 2 cut(s) 1060, 2699
AcuI CTGAAG 1 cut(s) 1179
AcvI CACGTG 1 cut(s) 2518
AcyI GRCGYC 3 cut(s) 167, 2472, 2695
AdeI CACNNNGTG 1 cut(s) 1463
AfaI GTAC 8 cut(s) 503, 551, 575, 1518, 2315, 2396, 2673, 2862
AfiI CCNNNNNNNGG 7 cut(s) 41, 551, 1499, 1571, 1784, 1785, 2233
AflIII ACRYGT 3 cut(s) 211, 1674, 2515
AgeI ACCGGT 1 cut(s) 1109
AhdI GACNNNNNGTC 1 cut(s) 2760
AjiI CACGTC 2 cut(s) 212, 2056
AjuI GAANNNNNNNTTGG 2 cut(s) 1117, 1149
Alw26I GTCTC 4 cut(s) 165, 248, 2285, 2463
AlwI GGATC 7 cut(s) 52, 351, 516, 735, 751, 2452, 2786
AlwNI CAGNNNCTG 1 cut(s) 1084
Ama87I CYCGRG 2 cut(s) 1549, 1899
Aor13HI TCCGGA 1 cut(s) 1564
ApeKI GCWGC 9 cut(s) 348, 1075, 1078, 1081, 1958, 1961, 1964, 2414, 2777
AsiGI ACCGGT 1 cut(s) 1109
Asp700I GAANNNNTTC 3 cut(s) 1031, 1985, 2901
AspLEI GCGC 2 cut(s) 2611, 2930
AspS9I GGNCC 4 cut(s) 1184, 1237, 1481, 2000
AsuC2I CCSGG 3 cut(s) 615, 1890, 2003
AvaI CYCGRG 2 cut(s) 1549, 1899
AvaII GGWCC 2 cut(s) 1184, 1481
BaeGI GKGCMC 1 cut(s) 2817
BanI GGYRCC 1 cut(s) 7
BbrPI CACGTG 1 cut(s) 2518
BbvI GCAGC 9 cut(s) 360, 1062, 1068, 1090, 1948, 1970, 1976, 2401, 2764
BccI CCATC 7 cut(s) 743, 913, 1501, 1518, 1593, 1642, 2069
BceAI ACGGC 2 cut(s) 2569, 2614
BciVI GTATCC 1 cut(s) 1146
BcnI CCSGG 3 cut(s) 615, 1890, 2003
BcoDI GTCTC 4 cut(s) 165, 248, 2285, 2463
BfaI CTAG 5 cut(s) 41, 204, 1224, 1949, 2180
BfmI CTRYAG 4 cut(s) 1076, 1497, 1962, 2778
BfuAI ACCTGC 1 cut(s) 810
BfuI GTATCC 1 cut(s) 1146
BglII AGATCT 1 cut(s) 648
BmcAI AGTACT 2 cut(s) 575, 1518
Bme1390I CCNGG 3 cut(s) 615, 1890, 2003
Bme18I GGWCC 2 cut(s) 1184, 1481
BmeRI GACNNNNNGTC 1 cut(s) 2760
BmeT110I CYCGRG 2 cut(s) 1549, 1899
BmgBI CACGTC 2 cut(s) 212, 2056
BmgT120I GGNCC 4 cut(s) 1184, 1237, 1481, 2000
BmiI GGNNCC 2 cut(s) 9, 1843
BmrFI CCNGG 3 cut(s) 615, 1890, 2003
BmrI ACTGGG 2 cut(s) 1244, 2563
BmsI GCATC 4 cut(s) 188, 1884, 2573, 2733
BmuI ACTGGG 2 cut(s) 1244, 2563
BoxI GACNNNNGTC 1 cut(s) 1605
BpmI CTGGAG 3 cut(s) 394, 1630, 2485
Bpu10I CCTNAGC 2 cut(s) 497, 1148
BpuEI CTTGAG 4 cut(s) 89, 508, 917, 2398
BpuMI CCSGG 3 cut(s) 615, 1890, 2003
BsaAI YACGTR 1 cut(s) 2518
BsaBI GATNNNNATC 1 cut(s) 1317
BsaHI GRCGYC 3 cut(s) 167, 2472, 2695
BsaI GGTCTC 1 cut(s) 2285
BsaJI CCNNGG 7 cut(s) 690, 833, 1713, 1898, 2116, 2206, 2795
BsaWI WCCGGW 3 cut(s) 1109, 1406, 1564
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 7 cut(s) 41, 551, 1499, 1571, 1784, 1785, 2233
Bse118I RCCGGY 3 cut(s) 282, 1109, 2701
Bse1I ACTGG 7 cut(s) 379, 404, 711, 750, 898, 1239, 2569
Bse3DI GCAATG 7 cut(s) 414, 598, 2342, 2604, 2835, 2844, 2856
Bse8I GATNNNNATC 1 cut(s) 1317
BseAI TCCGGA 1 cut(s) 1564
BseDI CCNNGG 7 cut(s) 690, 833, 1713, 1898, 2116, 2206, 2795
BseGI GGATG 8 cut(s) 754, 905, 1029, 1136, 1634, 2080, 2564, 2644
BseJI GATNNNNATC 1 cut(s) 1317
BseLI CCNNNNNNNGG 7 cut(s) 41, 551, 1499, 1571, 1784, 1785, 2233
BseMI GCAATG 7 cut(s) 414, 598, 2342, 2604, 2835, 2844, 2856
BseNI ACTGG 7 cut(s) 379, 404, 711, 750, 898, 1239, 2569
BseRI GAGGAG 4 cut(s) 2109, 2112, 2935, 2938
BseSI GKGCMC 1 cut(s) 2817
BseX3I CGGCCG 1 cut(s) 2699
BseXI GCAGC 9 cut(s) 360, 1062, 1068, 1090, 1948, 1970, 1976, 2401, 2764
BseYI CCCAGC 3 cut(s) 183, 1820, 1830
BsgI GTGCAG 1 cut(s) 1350
Bsh1236I CGCG 1 cut(s) 2928
Bsh1285I CGRYCG 1 cut(s) 2702
BshNI GGYRCC 1 cut(s) 7
BshTI ACCGGT 1 cut(s) 1109
BsiEI CGRYCG 1 cut(s) 2702
BsiHKCI CYCGRG 2 cut(s) 1549, 1899
BslFI GGGAC 3 cut(s) 524, 769, 1219
BslI CCNNNNNNNGG 7 cut(s) 41, 551, 1499, 1571, 1784, 1785, 2233
BsmAI GTCTC 4 cut(s) 165, 248, 2285, 2463
BsmBI CGTCTC 2 cut(s) 165, 2463
BsmFI GGGAC 3 cut(s) 524, 769, 1219
BsmI GAATGC 4 cut(s) 1260, 1348, 1650, 2069
Bso31I GGTCTC 1 cut(s) 2285
BsoBI CYCGRG 2 cut(s) 1549, 1899
Bsp1286I GDGCHC 1 cut(s) 2817
Bsp13I TCCGGA 1 cut(s) 1564
Bsp1407I TGTACA 1 cut(s) 2860
Bsp19I CCATGG 1 cut(s) 2206
BspACI CCGC 8 cut(s) 132, 459, 989, 1742, 1784, 1839, 2556, 2559
BspEI TCCGGA 1 cut(s) 1564
BspFNI CGCG 1 cut(s) 2928
BspHI TCATGA 2 cut(s) 763, 951
BspLI GGNNCC 2 cut(s) 9, 1843
BspMAI CTGCAG 3 cut(s) 1080, 1966, 2782
BspMI ACCTGC 1 cut(s) 810
BspPI GGATC 7 cut(s) 52, 351, 516, 735, 751, 2452, 2786
BspQI GCTCTTC 1 cut(s) 1721
BspT107I GGYRCC 1 cut(s) 7
BspTNI GGTCTC 1 cut(s) 2285
BsrBI CCGCTC 1 cut(s) 461
BsrDI GCAATG 7 cut(s) 414, 598, 2342, 2604, 2835, 2844, 2856
BsrFI RCCGGY 3 cut(s) 282, 1109, 2701
BsrGI TGTACA 1 cut(s) 2860
BsrI ACTGG 7 cut(s) 379, 404, 711, 750, 898, 1239, 2569
BssAI RCCGGY 3 cut(s) 282, 1109, 2701
BssECI CCNNGG 7 cut(s) 690, 833, 1713, 1898, 2116, 2206, 2795
BssNI GRCGYC 3 cut(s) 167, 2472, 2695
BssT1I CCWWGG 4 cut(s) 690, 833, 1713, 2206
Bst4CI ACNGT 5 cut(s) 605, 1580, 2676, 2873, 2996
Bst6I CTCTTC 4 cut(s) 953, 1444, 1721, 2956
BstACI GRCGYC 3 cut(s) 167, 2472, 2695
BstAUI TGTACA 1 cut(s) 2860
BstBAI YACGTR 1 cut(s) 2518
BstC8I GCNNGC 9 cut(s) 336, 860, 1365, 1379, 1934, 1946, 2271, 2590, 2802
BstDSI CCRYGG 2 cut(s) 2116, 2206
BstEII GGTNACC 1 cut(s) 2752
BstENI CCTNNNNNAGG 1 cut(s) 39
BstF5I GGATG 8 cut(s) 754, 905, 1029, 1136, 1634, 2080, 2564, 2644
BstFNI CGCG 1 cut(s) 2928
BstHHI GCGC 2 cut(s) 2611, 2930
BstMAI GTCTC 4 cut(s) 165, 248, 2285, 2463
BstMCI CGRYCG 1 cut(s) 2702
BstMWI GCNNNNNNNGC 6 cut(s) 802, 1081, 1964, 2411, 2519, 2598
BstNSI RCATGY 3 cut(s) 1678, 2351, 2578
BstPAI GACNNNNGTC 1 cut(s) 1605
BstPI GGTNACC 1 cut(s) 2752
BstSCI CCNGG 3 cut(s) 613, 1888, 2001
BstSFI CTRYAG 4 cut(s) 1076, 1497, 1962, 2778
BstSLI GKGCMC 1 cut(s) 2817
BstUI CGCG 1 cut(s) 2928
BstV1I GCAGC 9 cut(s) 360, 1062, 1068, 1090, 1948, 1970, 1976, 2401, 2764
BstX2I RGATCY 4 cut(s) 508, 648, 727, 756
BstXI CCANNNNNNTGG 1 cut(s) 386
BstYI RGATCY 4 cut(s) 508, 648, 727, 756
BstZI CGGCCG 1 cut(s) 2699
BsuI GTATCC 1 cut(s) 1146
BtgI CCRYGG 2 cut(s) 2116, 2206
BtrI CACGTC 2 cut(s) 212, 2056
BtsCI GGATG 8 cut(s) 754, 905, 1029, 1136, 1634, 2080, 2564, 2644
BtsI GCAGTG 1 cut(s) 2154
BtsIMutI CAGTG 6 cut(s) 85, 391, 1468, 2154, 2576, 2878
BveI ACCTGC 1 cut(s) 810
Cac8I GCNNGC 9 cut(s) 336, 860, 1365, 1379, 1934, 1946, 2271, 2590, 2802
CaiI CAGNNNCTG 1 cut(s) 1084
CciI TCATGA 2 cut(s) 763, 951
CfoI GCGC 2 cut(s) 2611, 2930
Cfr10I RCCGGY 3 cut(s) 282, 1109, 2701
Cfr13I GGNCC 4 cut(s) 1184, 1237, 1481, 2000
CseI GACGC 3 cut(s) 2056, 2684, 2783
Csp6I GTAC 8 cut(s) 502, 550, 574, 1517, 2314, 2395, 2672, 2861
CspAI ACCGGT 1 cut(s) 1109
CviQI GTAC 8 cut(s) 502, 550, 574, 1517, 2314, 2395, 2672, 2861
DraI TTTAAA 1 cut(s) 3016
DraIII CACNNNGTG 1 cut(s) 1463
DriI GACNNNNNGTC 1 cut(s) 2760
EaeI YGGCCR 2 cut(s) 1060, 2699
EagI CGGCCG 1 cut(s) 2699
Eam1104I CTCTTC 4 cut(s) 953, 1444, 1721, 2956
Eam1105I GACNNNNNGTC 1 cut(s) 2760
EarI CTCTTC 4 cut(s) 953, 1444, 1721, 2956
EciI GGCGGA 1 cut(s) 2574
EclXI CGGCCG 1 cut(s) 2699
Eco130I CCWWGG 4 cut(s) 690, 833, 1713, 2206
Eco147I AGGCCT 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 2285
Eco47I GGWCC 2 cut(s) 1184, 1481
Eco52I CGGCCG 1 cut(s) 2699
Eco57I CTGAAG 1 cut(s) 1179
Eco72I CACGTG 1 cut(s) 2518
Eco88I CYCGRG 2 cut(s) 1549, 1899
Eco91I GGTNACC 1 cut(s) 2752
EcoNI CCTNNNNNAGG 1 cut(s) 39
EcoO65I GGTNACC 1 cut(s) 2752
EcoRI GAATTC 2 cut(s) 148, 1540
EcoT14I CCWWGG 4 cut(s) 690, 833, 1713, 2206
EcoT22I ATGCAT 2 cut(s) 2623, 2856
ErhI CCWWGG 4 cut(s) 690, 833, 1713, 2206
Esp3I CGTCTC 2 cut(s) 165, 2463
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FaqI GGGAC 3 cut(s) 524, 769, 1219
FauI CCCGC 2 cut(s) 139, 1777
FauNDI CATATG 1 cut(s) 1940
FokI GGATG 8 cut(s) 761, 892, 1036, 1123, 1621, 2087, 2551, 2651
FspBI CTAG 5 cut(s) 41, 204, 1224, 1949, 2180
GlaI GCGC 2 cut(s) 2610, 2929
GsaI CCCAGC 3 cut(s) 187, 1824, 1834
GsuI CTGGAG 3 cut(s) 394, 1630, 2485
HgaI GACGC 3 cut(s) 2056, 2684, 2783
HhaI GCGC 2 cut(s) 2611, 2930
Hin1I GRCGYC 3 cut(s) 167, 2472, 2695
Hin6I GCGC 2 cut(s) 2609, 2928
HinP1I GCGC 2 cut(s) 2609, 2928
HincII GTYRAC 1 cut(s) 2353
HindII GTYRAC 1 cut(s) 2353
HindIII AAGCTT 2 cut(s) 856, 2156
Hpy166II GTNNAC 8 cut(s) 226, 721, 743, 2043, 2113, 2308, 2353, 2861
Hpy8I GTNNAC 8 cut(s) 226, 721, 743, 2043, 2113, 2308, 2353, 2861
Hpy99I CGWCG 5 cut(s) 160, 169, 2555, 2654, 2700
HpyAV CCTTC 9 cut(s) 671, 1203, 1762, 1807, 1969, 2026, 2471, 2714, 2973
HpyCH4III ACNGT 5 cut(s) 605, 1580, 2676, 2873, 2996
HpyCH4IV ACGT 8 cut(s) 167, 211, 984, 1331, 2055, 2472, 2517, 2652
HpyF10VI GCNNNNNNNGC 6 cut(s) 802, 1081, 1964, 2411, 2519, 2598
HpySE526I ACGT 8 cut(s) 167, 211, 984, 1331, 2055, 2472, 2517, 2652
Hsp92I GRCGYC 3 cut(s) 167, 2472, 2695
HspAI GCGC 2 cut(s) 2609, 2928
Kpn2I TCCGGA 1 cut(s) 1564
LguI GCTCTTC 1 cut(s) 1721
LmnI GCTCC 5 cut(s) 466, 2099, 2188, 2453, 2561
Lsp1109I GCAGC 9 cut(s) 360, 1062, 1068, 1090, 1948, 1970, 1976, 2401, 2764
LweI GCATC 4 cut(s) 188, 1884, 2573, 2733
MaeI CTAG 5 cut(s) 41, 204, 1224, 1949, 2180
MaeII ACGT 8 cut(s) 167, 211, 984, 1331, 2055, 2472, 2517, 2652
MaeIII GTNAC 4 cut(s) 24, 579, 2246, 2752
MbiI CCGCTC 1 cut(s) 461
MfeI CAATTG 3 cut(s) 2406, 2583, 2758
MflI RGATCY 4 cut(s) 508, 648, 727, 756
MhlI GDGCHC 1 cut(s) 2817
MlyI GAGTC 5 cut(s) 250, 1221, 1615, 1877, 2936
MmeI TCCRAC 9 cut(s) 125, 188, 315, 396, 903, 1317, 2031, 2363, 2505
Mph1103I ATGCAT 2 cut(s) 2623, 2856
MroI TCCGGA 1 cut(s) 1564
MroXI GAANNNNTTC 3 cut(s) 1031, 1985, 2901
MseI TTAA 5 cut(s) 137, 942, 2957, 3015, 3034
MslI CAYNNNNRTG 4 cut(s) 1673, 2569, 2810, 2849
MspA1I CMGCKG 3 cut(s) 183, 1081, 1961
MspR9I CCNGG 3 cut(s) 615, 1890, 2003
MunI CAATTG 3 cut(s) 2406, 2583, 2758
Mva1269I GAATGC 4 cut(s) 1260, 1348, 1650, 2069
MvnI CGCG 1 cut(s) 2928
MwoI GCNNNNNNNGC 6 cut(s) 802, 1081, 1964, 2411, 2519, 2598
NciI CCSGG 3 cut(s) 615, 1890, 2003
NcoI CCATGG 1 cut(s) 2206
NdeI CATATG 1 cut(s) 1940
NlaIV GGNNCC 2 cut(s) 9, 1843
NmeAIII GCCGAG 1 cut(s) 1038
NmuCI GTSAC 4 cut(s) 24, 579, 2246, 2752
NsiI ATGCAT 2 cut(s) 2623, 2856
NspI RCATGY 3 cut(s) 1678, 2351, 2578
PagI TCATGA 2 cut(s) 763, 951
PceI AGGCCT 1 cut(s) 276
PciI ACATGT 1 cut(s) 1674
PciSI GCTCTTC 1 cut(s) 1721
PcsI WCGNNNNNNNCGW 2 cut(s) 161, 164
PctI GAATGC 4 cut(s) 1260, 1348, 1650, 2069
PdmI GAANNNNTTC 3 cut(s) 1031, 1985, 2901
PfeI GAWTC 6 cut(s) 193, 685, 712, 1825, 2006, 2211
PflMI CCANNNNNTGG 1 cut(s) 2233
PinAI ACCGGT 1 cut(s) 1109
PleI GAGTC 5 cut(s) 249, 1221, 1614, 1877, 2936
PmaCI CACGTG 1 cut(s) 2518
PmlI CACGTG 1 cut(s) 2518
PpsI GAGTC 5 cut(s) 249, 1221, 1614, 1877, 2936
Ppu21I YACGTR 1 cut(s) 2518
PscI ACATGT 1 cut(s) 1674
PshAI GACNNNNGTC 1 cut(s) 1605
PspCI CACGTG 1 cut(s) 2518
PspEI GGTNACC 1 cut(s) 2752
PspFI CCCAGC 3 cut(s) 183, 1820, 1830
PspN4I GGNNCC 2 cut(s) 9, 1843
PspPI GGNCC 4 cut(s) 1184, 1237, 1481, 2000
PstI CTGCAG 3 cut(s) 1080, 1966, 2782
PstNI CAGNNNCTG 1 cut(s) 1084
PsuI RGATCY 4 cut(s) 508, 648, 727, 756
PvuII CAGCTG 3 cut(s) 183, 1081, 1961
RsaI GTAC 8 cut(s) 503, 551, 575, 1518, 2315, 2396, 2673, 2862
RsaNI GTAC 8 cut(s) 502, 550, 574, 1517, 2314, 2395, 2672, 2861
RseI CAYNNNNRTG 4 cut(s) 1673, 2569, 2810, 2849
SapI GCTCTTC 1 cut(s) 1721
SaqAI TTAA 5 cut(s) 137, 942, 2957, 3015, 3034
Sau96I GGNCC 4 cut(s) 1184, 1237, 1481, 2000
ScaI AGTACT 2 cut(s) 575, 1518
SchI GAGTC 5 cut(s) 250, 1221, 1615, 1877, 2936
ScrFI CCNGG 3 cut(s) 615, 1890, 2003
SduI GDGCHC 1 cut(s) 2817
SfaNI GCATC 4 cut(s) 188, 1884, 2573, 2733
SfcI CTRYAG 4 cut(s) 1076, 1497, 1962, 2778
SinI GGWCC 2 cut(s) 1184, 1481
SmiMI CAYNNNNRTG 4 cut(s) 1673, 2569, 2810, 2849
SmlI CTYRAG 4 cut(s) 104, 487, 932, 2377
SmoI CTYRAG 4 cut(s) 104, 487, 932, 2377
SseBI AGGCCT 1 cut(s) 276
SsiI CCGC 8 cut(s) 132, 459, 989, 1742, 1784, 1839, 2556, 2559
SspI AATATT 1 cut(s) 1920
SspMI CTAG 5 cut(s) 41, 204, 1224, 1949, 2180
StuI AGGCCT 1 cut(s) 276
StyD4I CCNGG 3 cut(s) 613, 1888, 2001
StyI CCWWGG 4 cut(s) 690, 833, 1713, 2206
TaaI ACNGT 5 cut(s) 605, 1580, 2676, 2873, 2996
TaiI ACGT 8 cut(s) 170, 214, 987, 1334, 2058, 2475, 2520, 2655
TaqI TCGA 7 cut(s) 196, 870, 2467, 2550, 2727, 2764, 2976
TatI WGTACW 5 cut(s) 573, 1516, 2313, 2671, 2860
TauI GCSGC 1 cut(s) 2559
TfiI GAWTC 6 cut(s) 193, 685, 712, 1825, 2006, 2211
Tru1I TTAA 5 cut(s) 137, 942, 2957, 3015, 3034
Tru9I TTAA 5 cut(s) 137, 942, 2957, 3015, 3034
TscAI CASTG 6 cut(s) 85, 391, 1468, 2154, 2576, 2878
TseFI GTSAC 4 cut(s) 24, 579, 2246, 2752
TseI GCWGC 9 cut(s) 348, 1075, 1078, 1081, 1958, 1961, 1964, 2414, 2777
Tsp45I GTSAC 4 cut(s) 24, 579, 2246, 2752
TspGWI ACGGA 2 cut(s) 567, 2340
TspRI CASTG 6 cut(s) 85, 391, 1468, 2154, 2576, 2878
Van91I CCANNNNNTGG 1 cut(s) 2233
VpaK11BI GGWCC 2 cut(s) 1184, 1481
XagI CCTNNNNNAGG 1 cut(s) 39
XbaI TCTAGA 1 cut(s) 203
XceI RCATGY 3 cut(s) 1678, 2351, 2578
XcmI CCANNNNNNNNNTGG 1 cut(s) 2204
XmnI GAANNNNTTC 3 cut(s) 1031, 1985, 2901
XspI CTAG 5 cut(s) 41, 204, 1224, 1949, 2180
ZraI GACGTC 2 cut(s) 168, 2473
ZrmI AGTACT 2 cut(s) 575, 1518
Zsp2I ATGCAT 2 cut(s) 2623, 2856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.