Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010572

Physical Location & Seq

Genomic Coordinates Reverse (-)

53560965 .. 53565859

Gene Structure 4895 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1435600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3000 bp

ATGATGGTCTTCCCCTTTATTGTCTTGACATTTTTCTCTCTTGTTGGCACATCTTGTTTTGCTTCTTCCTTGGTCAGTGATTTTCATGTTTTAGTCACTCTCAAGCAAGGCTTCCATTTCCCCGAACCGGCCTTAAGCACTTGGAATTCTTCGTCTCCACGATCAGTTTGTTCGTGGGTCGGAGTTCGGTGCAATAGAGGAAGAGTTGTTGCAGTGGACTTAACAGATTTCAGTATATCCGGCTCTGTATCTCCACTGATTTCAGGCCTCGACCGCCTCACCGACCTTTCTCTTGCAGGAAACAACTTCACTGGCAGCATTGCCATTGCCAATCTCACAAGCCTTCAGTTCCTCAACATATCCAACAACCAATTCAGTGGAGGACTGGATTGGAACTACTCAGGCATTGCCAATTTGGAAGTGTTTGATGCTTATAACAACAACTTCACTGCTCCTCTTCCACTTGGGATTTTGAGCTTGAACAAGCTCAGGTTCTTGGAACTTGGAGGCAATTTTTTCAGTGGGAAAATCCCAAAATCTTATGGCAATTTAGTCAGCTTGGAGTACTTGTCACTTGCCGGCAATGATCTCATTGGCGAAATTCCGGGCGAGTTGGGCAACCTCACTAACTTGAGAGAGCTTTACTTGGGATATTACAATGTTTTTGAAGGTGGGATTCCAAAGGAGTTTGGAAAATTGGTCAATATGGTTCACATGGATCTCTCGAGCTGCGAACTGGACGGGCGAATTCCCCGCGAGCTGGGGAATTTGAAGGCACTCGACACCCTTTACTTGCACATCAATCTTCTTTCAGGTTCAATTCCAAGGCATCTAGGCAACTTGACAAACTTGGTCAATCTTGATCTCTCCAACAATGCACTTACTGGTGAAATCCCATTTGAGTTTTCCACTCTCAAACAGCTCAAGCTTTTCAACCTCTTCATGAACAGATTGCATGGCTCGATTCCAGACTACGTCGCAGACTTGCCTAATTTGGAAACACTCGGGCTGTGGATGAACAACTTCACCGGGATCATCCCGCAGAAACTTGGCCAGAACGGGAAGCTTCAACTGCTCGATTTGTCCTCGAACAAGCTCACCGGCAAAATCCCACCAAACTTGTGTTCATCCAATCAGCTAAGAATACTAATTCTCTTGAAAAACTTTCTTTTCGGTCCAATCCCTCAAGGCTTGGGGACATGTTCTAGTCTCACTAGAGTGAGGTTGGGGCAGAATTACCTAAACGGTAGCATACCAAATGGCTTAATTTATTTGCCTCTGCTGAATTTAGCAGAGTTGCAAAACAATTACCTGTCTGGCATGTTGTCGGAGAATGCTAATAGCTCGTCGCAGCCAGCGAAATTAGGCCAGCTTAATCTGTCGGACAATCTCCTATCCGGTCCTCTACCATTTTCGCTTTCAAATTTCTCTTCCCTCCAAATCCTCCTACTTAGCAGAAATCAATTCTCCGGTCCAATCCCACCTTCAATAGGCCAACTCCATCAAGTACTAAAGCTTGATCTTCGCAGAAATTTGCTTTCTGGCGAAATCCCACCGGAAATCGGAAACTGTTTCCATCTCACTTACCTTGACATGAGCCAGAACAACCTGTCCGGCTCCATCCCACCCGAAATTTCCAACGTTCACATCCTGAATTACTTGAACATATCGAGAAACCACTTGAACCAAAACATTCCCAAGTCAATAGGAACCATGAAGAGCCTCACAATTGCTGATTTTTCCTTCAATGATTTCTCTGGAAAACTACCCGAATCAGGGCAGTTTGCTTTCTTTAATGCTTCTGCTTTCGCCGGAAATCCCCACCTTTGCGGCTCTTTCTTAAATAACCCTTGCAACATCACCACAATCACAAGCACACCCCGAAAAACCCACGGGGATTTCAAGCTAATCTTTGCATTAGGCCTGCTAATATGCTCACTGGTGTTTGCTGCGGCTGCTATCATCAAGGCCAAATCATTCAAAAGAAACGGTACAGATTCATGGAAAATAACTGCATTCCAGAAGCTGGAATTCACAATTGCTGACATCCTCGAATGCGTGATGGACGGGAATGTGATTGGAAGAGGGGGAGCAGGGATTGTTTACCATGGAAAAATGCCGAATGGGGTTGAAATTGCTGTGAAAAAGCTACTTGGTTTTGGACCGAATAGCCACGATCATGGCTTCAAAGCCGAAATCCAAACGTTAGGCAACATTCGGCATAGAAACATTGTGAGGCTGCTGGCATTTTGTTCGAACAAGGAGACCAATCTGCTTGTTTACGAGTACATGAGAAACGGAAGCTTGGGAGAGGCACTGCATGGGAAGAAGGGAGGTTTCTTGGGGTGGAATCTGAGGTACAAAATTGCAATTGAAGCTGCCAAAGGCTTGTGTTACCTTCACCATGATTGTTCTCCATTGATTCTTCACCGGGACGTCAAATCGAACAACATTTTGCTCAATTCAAGCTTTGAAGCGCACGTTGCGGATTTCGGGCTTGCCAAGTTCTTGATGGATGGGGGTACGTCTCAATGCATGTCTGCAATTGCAGGCTCTTATGGCTACATTGCACCAGAGTATGCATACACACTGAGGGTGGACGAGAAGAGCGATGTATATAGCTTTGGGGTTGTCCTGTTAGAGCTTCTGACAGGGCGTCGGCCGGTGGGCGAGTTCGGTGAAGGCGTGGACATTGTGCAGTGGTCGAAGAAAGCAACGAATTGTCGCAAAGAGGACGTTACAAATATTGTTGATCATAGGCTTACAGTATCTCTGCCCAAAGATGAAGCAATGCACATGTTCTTCATTGCAATGCTTTGCATCCAAGAAAACAGCGTCGAGAGGCCGACGATGAGAGAAGTCGTTCAGATGCTATCAGAGTTTCCTCGTCACTCTCCCGAATCCTTTCAGTCGTCCTCGTCCTTAGCCACTTCCCAAAACTTCGAGAATGTCGATAAAGACGGAAAATGTCCCAAGTTCAAACAAGATACTTTGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009908 GO:0010073 GO:0010075 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0033612 GO:0040008 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048507 GO:0048509 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0061458 GO:0065007 GO:0071944 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1000

Amino Acids

109.73

Weight (kDa)

7.56

Isoelectric Point (pI)

29.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	26 - 65	1.6e-08	Leucine rich repeat N-terminal domain
LRR_14	PF23598	108 - 216	4.1e-08	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	691 - 958	6.3e-44	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	691 - 956	6.6e-41	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010572)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g28640
malus_domestica	MD08G1241900.v1.1 MD15G1435600.v1.1
prunus_persica	Prupe.1G579200_v2.0.a1 Prupe.1G579200_v2.0.a1 Prupe.1G579200_v2.0.a1 Prupe.1G579200_v2.0.a1 Prupe.1G579200_v2.0.a1 Prupe.1G579200_v2.0.a1
pyrus_communis	pycom08g20980 pycom15g38430
rosa_chinensis	RchiOBHm_Chr7g0220681
rosa_laevigata	RLG00000002210
rosa_multiflora	Rmu_ssc0000189.1_g000002
rosa_roxburghii	Rroxscaffold_3G00239220
rosa_rugosa	Rorug07G0196100
rosa_samantha	Rh7AG336900 Rh7BG327800 Rh7CG354900 Rh7DG333500
rosa_wichuraiana	Rw7G028740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	435
AatII	GACGTC	1 cut(s)	2439
AccB7I	CCANNNNNTGG	1 cut(s)	2026
AccII	CGCG	1 cut(s)	756
AciI	CCGC	6 cut(s)	274, 754, 1040, 1830, 1952, 2486
AclI	AACGTT	2 cut(s)	1641, 2204
AclWI	GGATC	2 cut(s)	726, 1040
AcoI	YGGCCR	2 cut(s)	1051, 2660
AcsI	RAATTY	9 cut(s)	145, 600, 747, 766, 1285, 1423, 1531, 1632, 2030
AcuI	CTGAAG	1 cut(s)	329
AcyI	GRCGYC	2 cut(s)	2436, 2656
AfaI	GTAC	6 cut(s)	566, 1509, 1993, 2288, 2360, 2524
AfiI	CCNNNNNNNGG	7 cut(s)	127, 760, 1490, 1562, 1775, 1776, 2026
AflII	CTTAAG	1 cut(s)	133
AflIII	ACRYGT	2 cut(s)	1199, 2796
AhdI	GACNNNNNGTC	1 cut(s)	2655
AjuI	GAANNNNNNNTTGG	6 cut(s)	356, 388, 1108, 1140, 1468, 1500
AleI	CACNNNNGTG	1 cut(s)	1217
AloI	GAACNNNNNNTCC	2 cut(s)	1595, 1627
Alw26I	GTCTC	4 cut(s)	159, 1214, 2258, 2532
AlwI	GGATC	2 cut(s)	726, 1040
AlwNI	CAGNNNCTG	1 cut(s)	2026
Ama87I	CYCGRG	2 cut(s)	724, 1004
AoxI	GGCC	9 cut(s)	129, 265, 1051, 1366, 1492, 1921, 1968, 2660, 2843
ApeKI	GCWGC	7 cut(s)	315, 729, 1351, 1949, 1955, 2239, 2378
ApoI	RAATTY	9 cut(s)	145, 600, 747, 766, 1285, 1423, 1531, 1632, 2030
Asp700I	GAANNNNTTC	3 cut(s)	1022, 2862, 2904
AspLEI	GCGC	1 cut(s)	2479
AspS9I	GGNCC	4 cut(s)	1175, 1400, 1472, 2162
AsuC2I	CCSGG	3 cut(s)	606, 1030, 2432
AsuHPI	GGTGA	8 cut(s)	271, 899, 1018, 1090, 1852, 2393, 2420, 2690
AsuII	TTCGAA	1 cut(s)	2255
AvaI	CYCGRG	2 cut(s)	724, 1004
AvaII	GGWCC	4 cut(s)	1175, 1400, 1472, 2162
BaeI	ACNNNNGTAYC	1 cut(s)	2979
BalI	TGGCCA	1 cut(s)	1053
BbvI	GCAGC	7 cut(s)	327, 716, 1363, 1936, 1942, 2226, 2365
BccI	CCATC	6 cut(s)	1509, 1584, 1628, 2056, 2506, 2510
BcgI	CGANNNNNNTGC	2 cut(s)	2235, 2269
BclI	TGATCA	1 cut(s)	2752
BcnI	CCSGG	3 cut(s)	606, 1030, 2432
BcoDI	GTCTC	4 cut(s)	159, 1214, 2258, 2532
BfaI	CTAG	3 cut(s)	833, 1206, 1215
BfrI	CTTAAG	1 cut(s)	133
BisI	GCNGC	9 cut(s)	316, 730, 1352, 1831, 1950, 1953, 1956, 2240, 2379
BlsI	GCNGC	9 cut(s)	317, 731, 1353, 1832, 1951, 1954, 1957, 2241, 2380
BmcAI	AGTACT	2 cut(s)	566, 1509
Bme1390I	CCNGG	3 cut(s)	606, 1030, 2432
Bme18I	GGWCC	4 cut(s)	1175, 1400, 1472, 2162
BmeRI	GACNNNNNGTC	1 cut(s)	2655
BmeT110I	CYCGRG	2 cut(s)	724, 1004
BmgT120I	GGNCC	4 cut(s)	1175, 1400, 1472, 2162
BmiI	GGNNCC	2 cut(s)	1618, 1711
BmrFI	CCNGG	3 cut(s)	606, 1030, 2432
BmsI	GCATC	4 cut(s)	418, 838, 2829, 2859
Bpu10I	CCTNAGC	2 cut(s)	488, 2923
Bpu14I	TTCGAA	1 cut(s)	2255
BpuEI	CTTGAG	4 cut(s)	86, 652, 908, 1170
BpuMI	CCSGG	3 cut(s)	606, 1030, 2432
BsaHI	GRCGYC	2 cut(s)	2436, 2656
BsaI	GGTCTC	1 cut(s)	2258
BsaJI	CCNNGG	4 cut(s)	69, 824, 1891, 2107
BsaWI	WCCGGW	3 cut(s)	1397, 1469, 1555
BsaXI	ACNNNNNCTCC	2 cut(s)	554, 584
Bsc4I	CCNNNNNNNGG	7 cut(s)	127, 760, 1490, 1562, 1775, 1776, 2026
Bse118I	RCCGGY	4 cut(s)	127, 578, 1100, 2662
Bse1I	ACTGG	5 cut(s)	316, 390, 741, 889, 1944
Bse3DI	GCAATG	8 cut(s)	318, 324, 405, 589, 2565, 2796, 2805, 2817
BseDI	CCNNGG	4 cut(s)	69, 824, 1891, 2107
BseGI	GGATG	8 cut(s)	1020, 1035, 1127, 1620, 1647, 2046, 2521, 2820
BseLI	CCNNNNNNNGG	7 cut(s)	127, 760, 1490, 1562, 1775, 1776, 2026
BseMI	GCAATG	8 cut(s)	318, 324, 405, 589, 2565, 2796, 2805, 2817
BseMII	CTCAG	4 cut(s)	414, 502, 2345, 2582
BseNI	ACTGG	5 cut(s)	316, 390, 741, 889, 1944
BseRI	GAGGAG	1 cut(s)	444
BseX3I	CGGCCG	1 cut(s)	2660
BseXI	GCAGC	7 cut(s)	327, 716, 1363, 1936, 1942, 2226, 2365
BseYI	CCCAGC	1 cut(s)	760
BsgI	GTGCAG	1 cut(s)	2717
Bsh1236I	CGCG	1 cut(s)	756
Bsh1285I	CGRYCG	2 cut(s)	274, 2663
BshFI	GGCC	9 cut(s)	131, 267, 1053, 1368, 1494, 1923, 1970, 2662, 2845
BsiEI	CGRYCG	2 cut(s)	274, 2663
BsiHKCI	CYCGRG	2 cut(s)	724, 1004
BslFI	GGGAC	3 cut(s)	1210, 2447, 2955
BslI	CCNNNNNNNGG	7 cut(s)	127, 760, 1490, 1562, 1775, 1776, 2026
BsmAI	GTCTC	4 cut(s)	159, 1214, 2258, 2532
BsmBI	CGTCTC	2 cut(s)	159, 2532
BsmFI	GGGAC	3 cut(s)	1210, 2447, 2955
BsmI	GAATGC	3 cut(s)	1339, 2015, 2060
BsnI	GGCC	9 cut(s)	131, 267, 1053, 1368, 1494, 1923, 1970, 2662, 2845
Bso31I	GGTCTC	1 cut(s)	2258
BsoBI	CYCGRG	2 cut(s)	724, 1004
Bsp119I	TTCGAA	1 cut(s)	2255
Bsp143I	GATC	8 cut(s)	161, 586, 718, 862, 1032, 1519, 2176, 2752
Bsp19I	CCATGG	1 cut(s)	2107
BspACI	CCGC	6 cut(s)	274, 754, 1040, 1830, 1952, 2486
BspANI	GGCC	9 cut(s)	131, 267, 1053, 1368, 1494, 1923, 1970, 2662, 2845
BspCNI	CTCAG	4 cut(s)	413, 501, 2346, 2583
BspFNI	CGCG	1 cut(s)	756
BspHI	TCATGA	1 cut(s)	942
BspLI	GGNNCC	2 cut(s)	1618, 1711
BspPI	GGATC	2 cut(s)	726, 1040
BspQI	GCTCTTC	2 cut(s)	1712, 2600
BspT104I	TTCGAA	1 cut(s)	2255
BspTI	CTTAAG	1 cut(s)	133
BspTNI	GGTCTC	1 cut(s)	2258
BsrDI	GCAATG	8 cut(s)	318, 324, 405, 589, 2565, 2796, 2805, 2817
BsrFI	RCCGGY	4 cut(s)	127, 578, 1100, 2662
BsrI	ACTGG	5 cut(s)	316, 390, 741, 889, 1944
BssAI	RCCGGY	4 cut(s)	127, 578, 1100, 2662
BssECI	CCNNGG	4 cut(s)	69, 824, 1891, 2107
BssMI	GATC	8 cut(s)	161, 586, 718, 862, 1032, 1519, 2176, 2752
BssNI	GRCGYC	2 cut(s)	2436, 2656
BssT1I	CCWWGG	3 cut(s)	69, 824, 2107
Bst4CI	ACNGT	4 cut(s)	1247, 1571, 1991, 2767
Bst6I	CTCTTC	7 cut(s)	196, 462, 944, 1435, 1712, 2077, 2600
BstACI	GRCGYC	2 cut(s)	2436, 2656
BstAFI	CTTAAG	1 cut(s)	133
BstBI	TTCGAA	1 cut(s)	2255
BstC8I	GCNNGC	8 cut(s)	580, 758, 1356, 1370, 1925, 2244, 2499, 2551
BstDEI	CTNAG	7 cut(s)	400, 488, 1139, 1451, 2354, 2591, 2923
BstDSI	CCRYGG	2 cut(s)	1891, 2107
BstENI	CCTNNNNNAGG	1 cut(s)	1488
BstF5I	GGATG	8 cut(s)	1020, 1035, 1127, 1620, 1647, 2046, 2521, 2820
BstFNI	CGCG	1 cut(s)	756
BstHHI	GCGC	1 cut(s)	2479
BstKTI	GATC	8 cut(s)	164, 589, 721, 865, 1035, 1522, 2179, 2755
BstMAI	GTCTC	4 cut(s)	159, 1214, 2258, 2532
BstMBI	GATC	8 cut(s)	161, 586, 718, 862, 1032, 1519, 2176, 2752
BstMCI	CGRYCG	2 cut(s)	274, 2663
BstMWI	GCNNNNNNNGC	7 cut(s)	273, 615, 1072, 1955, 2375, 2483, 2559
BstNSI	RCATGY	4 cut(s)	1203, 1324, 2539, 2800
BstSCI	CCNGG	3 cut(s)	604, 1028, 2430
BstUI	CGCG	1 cut(s)	756
BstV1I	GCAGC	7 cut(s)	327, 716, 1363, 1936, 1942, 2226, 2365
BstX2I	RGATCY	1 cut(s)	718
BstXI	CCANNNNNNTGG	2 cut(s)	377, 2180
BstYI	RGATCY	1 cut(s)	718
BstZI	CGGCCG	1 cut(s)	2660
BsuRI	GGCC	9 cut(s)	131, 267, 1053, 1368, 1494, 1923, 1970, 2662, 2845
BtgI	CCRYGG	2 cut(s)	1891, 2107
BtgZI	GCGATG	1 cut(s)	2625
BtsCI	GGATG	8 cut(s)	1020, 1035, 1127, 1620, 1647, 2046, 2521, 2820
BtsI	GCAGTG	4 cut(s)	219, 447, 2315, 2705
Cac8I	GCNNGC	8 cut(s)	580, 758, 1356, 1370, 1925, 2244, 2499, 2551
CaiI	CAGNNNCTG	1 cut(s)	2026
CciI	TCATGA	1 cut(s)	942
CfoI	GCGC	1 cut(s)	2479
Cfr10I	RCCGGY	4 cut(s)	127, 578, 1100, 2662
Cfr13I	GGNCC	4 cut(s)	1175, 1400, 1472, 2162
CseI	GACGC	2 cut(s)	2645, 2824
Csp6I	GTAC	6 cut(s)	565, 1508, 1992, 2287, 2359, 2523
CspCI	CAANNNNNGTGG	2 cut(s)	1101, 1136
CviQI	GTAC	6 cut(s)	565, 1508, 1992, 2287, 2359, 2523
DdeI	CTNAG	7 cut(s)	400, 488, 1139, 1451, 2354, 2591, 2923
DpnI	GATC	8 cut(s)	163, 588, 720, 864, 1034, 1521, 2178, 2754
DpnII	GATC	8 cut(s)	161, 586, 718, 862, 1032, 1519, 2176, 2752
DriI	GACNNNNNGTC	1 cut(s)	2655
EaeI	YGGCCR	2 cut(s)	1051, 2660
EagI	CGGCCG	1 cut(s)	2660
Eam1104I	CTCTTC	7 cut(s)	196, 462, 944, 1435, 1712, 2077, 2600
Eam1105I	GACNNNNNGTC	1 cut(s)	2655
EarI	CTCTTC	7 cut(s)	196, 462, 944, 1435, 1712, 2077, 2600
EclXI	CGGCCG	1 cut(s)	2660
Eco130I	CCWWGG	3 cut(s)	69, 824, 2107
Eco147I	AGGCCT	2 cut(s)	267, 1923
Eco31I	GGTCTC	1 cut(s)	2258
Eco47I	GGWCC	4 cut(s)	1175, 1400, 1472, 2162
Eco52I	CGGCCG	1 cut(s)	2660
Eco57I	CTGAAG	1 cut(s)	329
Eco88I	CYCGRG	2 cut(s)	724, 1004
EcoNI	CCTNNNNNAGG	1 cut(s)	1488
EcoRI	GAATTC	3 cut(s)	145, 747, 2030
EcoT14I	CCWWGG	3 cut(s)	69, 824, 2107
EcoT22I	ATGCAT	2 cut(s)	2537, 2584
ErhI	CCWWGG	3 cut(s)	69, 824, 2107
Esp3I	CGTCTC	2 cut(s)	159, 2532
FalI	AAGNNNNNCTT	4 cut(s)	95, 127, 1895, 1927
FaqI	GGGAC	3 cut(s)	1210, 2447, 2955
FauI	CCCGC	2 cut(s)	761, 1047
FbaI	TGATCA	1 cut(s)	2752
Fnu4HI	GCNGC	9 cut(s)	316, 730, 1352, 1831, 1950, 1953, 1956, 2240, 2379
FokI	GGATG	8 cut(s)	1022, 1027, 1114, 1607, 1634, 2033, 2528, 2807
Fsp4HI	GCNGC	9 cut(s)	316, 730, 1352, 1831, 1950, 1953, 1956, 2240, 2379
FspBI	CTAG	3 cut(s)	833, 1206, 1215
GlaI	GCGC	1 cut(s)	2478
GluI	GCNGC	9 cut(s)	316, 730, 1352, 1831, 1950, 1953, 1956, 2240, 2379
GsaI	CCCAGC	1 cut(s)	764
HaeIII	GGCC	9 cut(s)	131, 267, 1053, 1368, 1494, 1923, 1970, 2662, 2845
HgaI	GACGC	2 cut(s)	2645, 2824
HhaI	GCGC	1 cut(s)	2479
Hin1I	GRCGYC	2 cut(s)	2436, 2656
Hin6I	GCGC	1 cut(s)	2477
HinP1I	GCGC	1 cut(s)	2477
HindIII	AAGCTT	5 cut(s)	926, 1064, 1514, 2302, 2467
HinfI	GANTC	7 cut(s)	676, 964, 1772, 1997, 2350, 2422, 2900
HphI	GGTGA	8 cut(s)	271, 899, 1018, 1090, 1852, 2393, 2420, 2690
Hpy166II	GTNNAC	7 cut(s)	217, 712, 1645, 2104, 2281, 2599, 2689
Hpy188I	TCNGA	8 cut(s)	182, 1330, 1384, 1565, 2355, 2649, 2868, 2878
Hpy8I	GTNNAC	7 cut(s)	217, 712, 1645, 2104, 2281, 2599, 2689
Hpy99I	CGWCG	5 cut(s)	980, 1351, 2661, 2840, 2851
HpyAV	CCTTC	8 cut(s)	353, 662, 766, 1494, 1753, 2323, 2408, 2675
HpyCH4III	ACNGT	4 cut(s)	1247, 1571, 1991, 2767
HpyCH4IV	ACGT	7 cut(s)	975, 1641, 2204, 2436, 2481, 2525, 2736
HpyF10VI	GCNNNNNNNGC	7 cut(s)	273, 615, 1072, 1955, 2375, 2483, 2559
HpyF3I	CTNAG	7 cut(s)	400, 488, 1139, 1451, 2354, 2591, 2923
HpySE526I	ACGT	7 cut(s)	975, 1641, 2204, 2436, 2481, 2525, 2736
Hsp92I	GRCGYC	2 cut(s)	2436, 2656
HspAI	GCGC	1 cut(s)	2477
KroI	GCCGGC	1 cut(s)	578
KroNI	GCCGGC	1 cut(s)	580
Ksp22I	TGATCA	1 cut(s)	2752
Kzo9I	GATC	8 cut(s)	161, 586, 718, 862, 1032, 1519, 2176, 2752
LguI	GCTCTTC	2 cut(s)	1712, 2600
LmnI	GCTCC	3 cut(s)	457, 1622, 2090
Lsp1109I	GCAGC	7 cut(s)	327, 716, 1363, 1936, 1942, 2226, 2365
LweI	GCATC	4 cut(s)	418, 838, 2829, 2859
MaeI	CTAG	3 cut(s)	833, 1206, 1215
MaeII	ACGT	7 cut(s)	975, 1641, 2204, 2436, 2481, 2525, 2736
MaeIII	GTNAC	5 cut(s)	94, 570, 2393, 2737, 2888
MalI	GATC	8 cut(s)	163, 588, 720, 864, 1034, 1521, 2178, 2754
MboI	GATC	8 cut(s)	161, 586, 718, 862, 1032, 1519, 2176, 2752
MfeI	CAATTG	4 cut(s)	1728, 2037, 2370, 2544
MflI	RGATCY	1 cut(s)	718
MlsI	TGGCCA	1 cut(s)	1053
MluNI	TGGCCA	1 cut(s)	1053
MmeI	TCCRAC	6 cut(s)	160, 387, 894, 1308, 1362, 1662
Mox20I	TGGCCA	1 cut(s)	1053
Mph1103I	ATGCAT	2 cut(s)	2537, 2584
MroNI	GCCGGC	1 cut(s)	578
MroXI	GAANNNNTTC	3 cut(s)	1022, 2862, 2904
MscI	TGGCCA	1 cut(s)	1053
MseI	TTAA	7 cut(s)	134, 221, 1265, 1374, 1794, 1841, 2998
MslI	CAYNNNNRTG	3 cut(s)	1217, 2178, 2810
Msp20I	TGGCCA	1 cut(s)	1053
MspCI	CTTAAG	1 cut(s)	133
MspR9I	CCNGG	3 cut(s)	606, 1030, 2432
MunI	CAATTG	4 cut(s)	1728, 2037, 2370, 2544
Mva1269I	GAATGC	3 cut(s)	1339, 2015, 2060
MvnI	CGCG	1 cut(s)	756
MwoI	GCNNNNNNNGC	7 cut(s)	273, 615, 1072, 1955, 2375, 2483, 2559
NaeI	GCCGGC	1 cut(s)	580
NciI	CCSGG	3 cut(s)	606, 1030, 2432
NcoI	CCATGG	1 cut(s)	2107
NdeII	GATC	8 cut(s)	161, 586, 718, 862, 1032, 1519, 2176, 2752
NgoMIV	GCCGGC	1 cut(s)	578
NlaIV	GGNNCC	2 cut(s)	1618, 1711
NmuCI	GTSAC	3 cut(s)	94, 570, 2888
NsiI	ATGCAT	2 cut(s)	2537, 2584
NspI	RCATGY	4 cut(s)	1203, 1324, 2539, 2800
NspV	TTCGAA	1 cut(s)	2255
OliI	CACNNNNGTG	1 cut(s)	1217
PaeR7I	CTCGAG	1 cut(s)	724
PagI	TCATGA	1 cut(s)	942
PceI	AGGCCT	2 cut(s)	267, 1923
PciI	ACATGT	2 cut(s)	1199, 2796
PciSI	GCTCTTC	2 cut(s)	1712, 2600
PcsI	WCGNNNNNNNCGW	4 cut(s)	1355, 2607, 2682, 2949
PctI	GAATGC	3 cut(s)	1339, 2015, 2060
PdiI	GCCGGC	1 cut(s)	580
PdmI	GAANNNNTTC	3 cut(s)	1022, 2862, 2904
PfeI	GAWTC	7 cut(s)	676, 964, 1772, 1997, 2350, 2422, 2900
PflFI	GACNNNGTC	1 cut(s)	974
PflMI	CCANNNNNTGG	1 cut(s)	2026
PkrI	GCNGC	9 cut(s)	317, 731, 1353, 1832, 1951, 1954, 1957, 2241, 2380
PscI	ACATGT	2 cut(s)	1199, 2796
PsiI	TTATAA	1 cut(s)	435
Psp1406I	AACGTT	2 cut(s)	1641, 2204
PspFI	CCCAGC	1 cut(s)	760
PspN4I	GGNNCC	2 cut(s)	1618, 1711
PspPI	GGNCC	4 cut(s)	1175, 1400, 1472, 2162
PstNI	CAGNNNCTG	1 cut(s)	2026
PsuI	RGATCY	1 cut(s)	718
PsyI	GACNNNGTC	1 cut(s)	974
RsaI	GTAC	6 cut(s)	566, 1509, 1993, 2288, 2360, 2524
RsaNI	GTAC	6 cut(s)	565, 1508, 1992, 2287, 2359, 2523
RseI	CAYNNNNRTG	3 cut(s)	1217, 2178, 2810
SapI	GCTCTTC	2 cut(s)	1712, 2600
SaqAI	TTAA	7 cut(s)	134, 221, 1265, 1374, 1794, 1841, 2998
SatI	GCNGC	9 cut(s)	316, 730, 1352, 1831, 1950, 1953, 1956, 2240, 2379
Sau3AI	GATC	8 cut(s)	161, 586, 718, 862, 1032, 1519, 2176, 2752
Sau96I	GGNCC	4 cut(s)	1175, 1400, 1472, 2162
ScaI	AGTACT	2 cut(s)	566, 1509
ScrFI	CCNGG	3 cut(s)	606, 1030, 2432
SfaNI	GCATC	4 cut(s)	418, 838, 2829, 2859
Sfr274I	CTCGAG	1 cut(s)	724
SfuI	TTCGAA	1 cut(s)	2255
SinI	GGWCC	4 cut(s)	1175, 1400, 1472, 2162
SlaI	CTCGAG	1 cut(s)	724
SmiMI	CAYNNNNRTG	3 cut(s)	1217, 2178, 2810
SmlI	CTYRAG	6 cut(s)	101, 133, 631, 724, 923, 1185
SmoI	CTYRAG	6 cut(s)	101, 133, 631, 724, 923, 1185
SseBI	AGGCCT	2 cut(s)	267, 1923
SsiI	CCGC	6 cut(s)	274, 754, 1040, 1830, 1952, 2486
SspI	AATATT	1 cut(s)	2746
SspMI	CTAG	3 cut(s)	833, 1206, 1215
StuI	AGGCCT	2 cut(s)	267, 1923
StyD4I	CCNGG	3 cut(s)	604, 1028, 2430
StyI	CCWWGG	3 cut(s)	69, 824, 2107
TaaI	ACNGT	4 cut(s)	1247, 1571, 1991, 2767
TaiI	ACGT	7 cut(s)	978, 1644, 2207, 2439, 2484, 2528, 2739
TaqII	GACCGA	2 cut(s)	1163, 2179
TatI	WGTACW	3 cut(s)	564, 1507, 2286
TauI	GCSGC	2 cut(s)	1833, 1955
TfiI	GAWTC	7 cut(s)	676, 964, 1772, 1997, 2350, 2422, 2900
Tru1I	TTAA	7 cut(s)	134, 221, 1265, 1374, 1794, 1841, 2998
Tru9I	TTAA	7 cut(s)	134, 221, 1265, 1374, 1794, 1841, 2998
TseFI	GTSAC	3 cut(s)	94, 570, 2888
TseI	GCWGC	7 cut(s)	315, 729, 1351, 1949, 1955, 2239, 2378
Tsp45I	GTSAC	3 cut(s)	94, 570, 2888
TspDTI	ATGAA	9 cut(s)	74, 931, 959, 1031, 1116, 1730, 1989, 2794, 2799
TspGWI	ACGGA	2 cut(s)	2313, 2976
Tth111I	GACNNNGTC	1 cut(s)	974
Van91I	CCANNNNNTGG	1 cut(s)	2026
Vha464I	CTTAAG	1 cut(s)	133
VpaK11BI	GGWCC	4 cut(s)	1175, 1400, 1472, 2162
XagI	CCTNNNNNAGG	1 cut(s)	1488
XapI	RAATTY	9 cut(s)	145, 600, 747, 766, 1285, 1423, 1531, 1632, 2030
XceI	RCATGY	4 cut(s)	1203, 1324, 2539, 2800
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2509
XhoI	CTCGAG	1 cut(s)	724
XmnI	GAANNNNTTC	3 cut(s)	1022, 2862, 2904
XspI	CTAG	3 cut(s)	833, 1206, 1215
ZraI	GACGTC	1 cut(s)	2437
ZrmI	AGTACT	2 cut(s)	566, 1509
Zsp2I	ATGCAT	2 cut(s)	2537, 2584

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.