RchiOBHm_Chr7g0220681

leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010572
Physical Location & Seq
Forward (+)
39861892 .. 39866691
4800 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19751

Sequence Viewer

Length: 3027 bp
ATGGTGGTGCCTTTCATTTTGTTGACACTTTTCTCTGTTCTAGGAACCCCCTGTTTTGCTTCTTCTTTGGTCAGTGATTTTCATGTCTTAATCGCACTCAAGCAAGGCTTCAAATTCTCCGAACCAGCTTTAAGTAGTTGGAATTCTTCGTCTCCAAGATCAGTTTGTTCCAGCTGGGTTGGAATTCGATGCTCTAGAGGACGAGTTGTTGCAGTGGACTTGACAGGTTTCAGTCTCGCTGGTTCTGTTTCGCCTATCATTTCAGGCCTTGACCGGCTTTCCGACCTCTCTCTAGCCGGAAACAACTTGTCAGGAAGCATTGAGATTGCAAATTTGGGCAGCCTCCGATCCCTCAACATCTCCGACAACCAGTTCAGTGGGGGCTTAGACTGGAACTACTCCAGCATTGCCAACTTGGAGGTTTTTGATGTCTATGATAACAACTTGACTGCTCCACTTCCACTTGGGATTTTGAGCTTGAATAAGCTCAGGTACTTGGATCTCGGAGGCAACTTCTTCTATGGGAAAGTCCCACCATTATACGGCAAACTAGTCAGCTTGGAGTACTTGTCAGTTTCAGGCAATGATCTTAACGGTGAAATTCCAGGTGCGTTGGGCAATCTCACCAACCTGAGACAACTTTACCTGGGCTACTACAATGTTTTCGAAGGTGGGATTCCGAAGCAGTTTGGGAAATTGGTGAATCTTGTTCACATGGATCTTTCAAGTTGCGAATTGGACGGACAAATCCCTCATGAGTTGGGGAATTTGAAGTCCCTTGACACCCTTTATTTGCATATCAATCTGCTCTCAGGCTCAATTCCAAGGCAGTTGGGCAACTTGACAAACTTGCTGTATTTCGACCTCTCCAACAATGCACTTACTGGTGAAATTCCATCCGAGTTTTCAAATCTCAAACAGCTCAAGCTCTTTAACCTCTTCATGAACAGATTGCACGGGTCTATTCCAGACTACGTCGCGGATTTGCCTGATTTGGACACCCTGGGGCTGTGGATGAACAACTTCACCGGCATAATTCCTCCGAAACTCGGCCAAAATGGGAAGCTGCAGCTGCTCGATTTGTCCTCAAATAAGCTCACCGGTAAAATCCCGCCAAACTTGTGTTCATCCAATCAGCTAAGGATACTAATTCTGTTAAAAAACTTTCTGTTTGGACCAATCCCTTTAGGCTTGGGGACTTGTTCTAGTCTGACTAGAGTCAGACTGGGGCAGAATTACCTGAATGGTAGCATTCCAAATGGCTTAATTTACTTGCCTCTGCTCAATTTAGCAGAGTTGCAAGATAATTATCTTTCCGGCACCTTGTCGGAGAATGCCAACAGTTCATCACAGCCTGCCCTATTAGGCCAGCTTAATCTGTCAAACAATCTCTTATCCGGTCCTATACCATATTCGATTTCAAATTTCTCTTCCCTCCAAATCCTCTCACTCAGTGGAAATCACCTCTCGGGTCCAGTCCCACCATCTATAGGCCAACTCCATCAAGTACTAAACCTTGATCTTAGCCGGAATTCACTCTCGGGTGAAGTTCCTCCGGAAATTGGAAACTGTTTCCATCTGACTTACCTTGACATGAGTCAGAACAACCTCTCTGGCTCAATCCCATCCGAAATCTCCAACATTCATATACTGAATTACATCAACATGTCAAGAAACCACTTGAACCAGAGCATACCCAAATCCTTGGGAACCATGAAGAGCCTCACAACTGCTGATTTTTCCTTCAATGACTTGTCGGGTAAACTACCCGAATCCGGGCAGTTTGCCTTCTTCAATGCTTCCTCTTTCGCTGGGAATCCCCATCTTTGCGGTTCCCTCCTAAACAATCCTTGCAATTTCACCACAATCACAAACACACCCGGAAAACGTCGGGCAGATTTCAAGCTAATCTTCGCATTAGGCTTGCTCATATGCTCCCTAGTATTTGCTGCTGCAGCCATAATGAAGGCCAAATCGTTCACAAGAAAGGGCCCAGAATCATGGAAAATGACAGCATTTCAGAAGGTGGAATTCACAATGTCGGACGTGCTGGAATGCGTGAAAGATGGAAATGTGATTGGAAGAGGAGGAGCAGGAATTGTTTACCATGGGAAAATGCCAAACGGGGTTGAAATTGCAGTGAAAAAGCTTCTTGGTTTTGGGCTTGGTCTAGCTCCGAACAGCACCCATGACGACCACGGATTCAGAGCCGAAATCCAAACACTGGGCAACATTCGTCATAGAAACATTGTGAGATTGCTGGCATTTTGCTCAAACAAGGACACCAATCTGCTTGTTTACGAGTACATGAGAAATGGAAGCTTGGGAGCGGCATTGCATGTCAACAACAAAAAAGGCGGGTTATTCTTGAGTTGGAATTTGAGGTACAAAATTGCAATTGAAGCTGCCAAAGGGTTGTGCTATCTCCACCACGACTGCTCCCCGTTGATCCTTCACCGAGACGTCAAATCCAACAATATTTTGCTCAATTCCAGCTTCGAAGCACACGTGGCGGATTTCGGGCTCGCCAAGTTCTTGATCGACGGCGGCGGAGCATCCCAGTGCATGTCAGCAATTGCAGGCTCTTATGGCTACATTGCGCCTGAGTATGCGTACACACTGAGGGTGGACGAGAAGAGCGACGTATACAGTTTCGGTGTTGTCTTGTTAGAGCTTCTAACAGGGCGTCGGCCGGTCGGTGAGTTTGGCGAAGGCGTCGACATAGTCCAATGGTGTAAAAAAGTGACCAATTGTCGCAAACAAGACGCCGCAAACATTGTTGATCCAAGGCTGGCCATATCTGTGCCCAAAGATGAAGCAATGCACTTGTTTTTCATTGCAATGCTTTGCGTACAAGAAAACAGTGTTGAGAGGCCAACAATGAGAGAAGTTGTTCAAATGTTGTCAGAATTTCCTCGCGCCCATTCTCCTGACTCCTCCTCTTCTTTAATCACTTCCCAACAATTCAATAACAAACAACAAGATGGTAAATGTCCTCCTAAATTTAAACAACATATTTTAGTTTAA

Protein Analysis

1008

Amino Acids

109.83

Weight (kDa)

7.98

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 65 2e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 115 - 267 3.4e-12 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 690 - 968 5.2e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 690 - 966 1.6e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2466
AccB1I GGYRCC 2 cut(s) 7, 1319
AccB7I CCANNNNNTGG 1 cut(s) 2224
AccBSI CCGCTC 1 cut(s) 2330
AccI GTMKAC 2 cut(s) 2646, 2718
AccII CGCG 2 cut(s) 980, 2919
AccIII TCCGGA 1 cut(s) 1555
AciI CCGC 9 cut(s) 980, 1112, 1830, 2330, 2358, 2513, 2547, 2550, 2769
AclWI GGATC 5 cut(s) 342, 507, 726, 2443, 2777
AcoI YGGCCR 3 cut(s) 1051, 2690, 2793
AcvI CACGTG 1 cut(s) 2509
AcyI GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
AdeI CACNNNGTG 1 cut(s) 1454
AfaI GTAC 7 cut(s) 494, 566, 1509, 2306, 2387, 2615, 2853
AfiI CCNNNNNNNGG 7 cut(s) 542, 1049, 1490, 1562, 1775, 1776, 2224
AflIII ACRYGT 2 cut(s) 1665, 2506
AgeI ACCGGT 1 cut(s) 1100
AhdI GACNNNNNGTC 1 cut(s) 2751
AhlI ACTAGT 1 cut(s) 550
AjiI CACGTC 1 cut(s) 2047
AjnI CCWGG 3 cut(s) 604, 645, 1002
AjuI GAANNNNNNNTTGG 2 cut(s) 1108, 1140
Alw26I GTCTC 4 cut(s) 156, 239, 628, 2454
AlwI GGATC 5 cut(s) 342, 507, 726, 2443, 2777
Ama87I CYCGRG 2 cut(s) 1468, 1540
Aor13HI TCCGGA 1 cut(s) 1555
AoxI GGCC 9 cut(s) 265, 1051, 1366, 1492, 1968, 1990, 2690, 2793, 2873
ApaI GGGCCC 1 cut(s) 1994
ApeKI GCWGC 8 cut(s) 339, 1066, 1069, 1072, 1949, 1952, 1955, 2405
AsiGI ACCGGT 1 cut(s) 1100
Asp700I GAANNNNTTC 2 cut(s) 1022, 2892
AspLEI GCGC 2 cut(s) 2602, 2921
AspS9I GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
AsuC2I CCSGG 2 cut(s) 1777, 1881
AsuII TTCGAA 2 cut(s) 666, 2499
AvaI CYCGRG 2 cut(s) 1468, 1540
AvaII GGWCC 3 cut(s) 1175, 1400, 1472
BaeGI GKGCMC 2 cut(s) 1994, 2808
BalI TGGCCA 1 cut(s) 2795
BanI GGYRCC 2 cut(s) 7, 1319
BanII GRGCYC 2 cut(s) 1994, 2526
BbrPI CACGTG 1 cut(s) 2509
BbvI GCAGC 8 cut(s) 351, 1053, 1059, 1081, 1936, 1939, 1967, 2392
BccI CCATC 8 cut(s) 904, 1492, 1509, 1584, 1633, 1830, 2060, 2978
BceAI ACGGC 2 cut(s) 559, 2560
BciT130I CCWGG 3 cut(s) 606, 647, 1004
BciVI GTATCC 1 cut(s) 1137
BcnI CCSGG 2 cut(s) 1777, 1881
BcoDI GTCTC 4 cut(s) 156, 239, 628, 2454
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 8 cut(s) 41, 195, 293, 551, 1206, 1215, 1940, 2171
BfmI CTRYAG 3 cut(s) 1067, 1488, 1953
BfuI GTATCC 1 cut(s) 1137
BmcAI AGTACT 2 cut(s) 566, 1509
Bme1390I CCNGG 5 cut(s) 606, 647, 1004, 1777, 1881
Bme18I GGWCC 3 cut(s) 1175, 1400, 1472
BmeRI GACNNNNNGTC 1 cut(s) 2751
BmeT110I CYCGRG 2 cut(s) 1468, 1540
BmgBI CACGTC 1 cut(s) 2047
BmgT120I GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
BmiI GGNNCC 7 cut(s) 9, 46, 1321, 1473, 1711, 1834, 1992
BmrFI CCNGG 5 cut(s) 606, 647, 1004, 1777, 1881
BmrI ACTGGG 3 cut(s) 1235, 2234, 2554
BmsI GCATC 2 cut(s) 179, 2564
BmuI ACTGGG 3 cut(s) 1235, 2234, 2554
BoxI GACNNNNGTC 2 cut(s) 1217, 1596
BpmI CTGGAG 1 cut(s) 385
Bpu10I CCTNAGC 2 cut(s) 488, 1139
Bpu14I TTCGAA 2 cut(s) 666, 2499
BpuEI CTTGAG 3 cut(s) 83, 908, 2389
BpuMI CCSGG 2 cut(s) 1777, 1881
BsaAI YACGTR 1 cut(s) 2509
BsaBI GATNNNNATC 1 cut(s) 1308
BsaHI GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
BsaJI CCNNGG 8 cut(s) 646, 824, 1002, 1003, 1704, 2107, 2197, 2786
BsaWI WCCGGW 3 cut(s) 1100, 1397, 1555
BsaXI ACNNNNNCTCC 4 cut(s) 554, 584, 2423, 2453
Bsc4I CCNNNNNNNGG 7 cut(s) 542, 1049, 1490, 1562, 1775, 1776, 2224
Bse118I RCCGGY 4 cut(s) 273, 1028, 1100, 2692
Bse1I ACTGG 7 cut(s) 370, 395, 889, 1230, 1475, 2229, 2560
Bse3DI GCAATG 7 cut(s) 405, 589, 2333, 2595, 2826, 2835, 2847
Bse8I GATNNNNATC 1 cut(s) 1308
BseAI TCCGGA 1 cut(s) 1555
BseBI CCWGG 3 cut(s) 606, 647, 1004
BseDI CCNNGG 8 cut(s) 646, 824, 1002, 1003, 1704, 2107, 2197, 2786
BseGI GGATG 5 cut(s) 896, 1020, 1127, 1625, 2555
BseJI GATNNNNATC 1 cut(s) 1308
BseLI CCNNNNNNNGG 7 cut(s) 542, 1049, 1490, 1562, 1775, 1776, 2224
BseMI GCAATG 7 cut(s) 405, 589, 2333, 2595, 2826, 2835, 2847
BseMII CTCAG 6 cut(s) 502, 623, 825, 1465, 2595, 2612
BseNI ACTGG 7 cut(s) 370, 395, 889, 1230, 1475, 2229, 2560
BseRI GAGGAG 4 cut(s) 2100, 2103, 2926, 2929
BseSI GKGCMC 2 cut(s) 1994, 2808
BseX3I CGGCCG 1 cut(s) 2690
BseXI GCAGC 8 cut(s) 351, 1053, 1059, 1081, 1936, 1939, 1967, 2392
BseYI CCCAGC 2 cut(s) 174, 1811
Bsh1236I CGCG 2 cut(s) 980, 2919
Bsh1285I CGRYCG 2 cut(s) 2693, 2697
BshFI GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2692, 2795, 2875
BshNI GGYRCC 2 cut(s) 7, 1319
BshTI ACCGGT 1 cut(s) 1100
BsiEI CGRYCG 2 cut(s) 2693, 2697
BsiHKCI CYCGRG 2 cut(s) 1468, 1540
BslFI GGGAC 4 cut(s) 515, 760, 1210, 1463
BslI CCNNNNNNNGG 7 cut(s) 542, 1049, 1490, 1562, 1775, 1776, 2224
BsmAI GTCTC 4 cut(s) 156, 239, 628, 2454
BsmBI CGTCTC 2 cut(s) 156, 2454
BsmFI GGGAC 4 cut(s) 515, 760, 1210, 1463
BsmI GAATGC 3 cut(s) 1251, 1339, 2060
BsnI GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2692, 2795, 2875
BsoBI CYCGRG 2 cut(s) 1468, 1540
Bsp119I TTCGAA 2 cut(s) 666, 2499
Bsp120I GGGCCC 1 cut(s) 1990
Bsp1286I GDGCHC 3 cut(s) 1994, 2526, 2808
Bsp13I TCCGGA 1 cut(s) 1555
Bsp143I GATC 9 cut(s) 158, 347, 499, 586, 718, 1519, 2448, 2538, 2782
Bsp19I CCATGG 1 cut(s) 2107
BspACI CCGC 9 cut(s) 980, 1112, 1830, 2330, 2358, 2513, 2547, 2550, 2769
BspANI GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2692, 2795, 2875
BspCNI CTCAG 6 cut(s) 501, 624, 824, 1464, 2596, 2613
BspEI TCCGGA 1 cut(s) 1555
BspFNI CGCG 2 cut(s) 980, 2919
BspHI TCATGA 2 cut(s) 754, 942
BspLI GGNNCC 7 cut(s) 9, 46, 1321, 1473, 1711, 1834, 1992
BspMAI CTGCAG 2 cut(s) 1071, 1957
BspPI GGATC 5 cut(s) 342, 507, 726, 2443, 2777
BspQI GCTCTTC 2 cut(s) 1712, 2630
BspT104I TTCGAA 2 cut(s) 666, 2499
BspT107I GGYRCC 2 cut(s) 7, 1319
BsrBI CCGCTC 1 cut(s) 2330
BsrDI GCAATG 7 cut(s) 405, 589, 2333, 2595, 2826, 2835, 2847
BsrFI RCCGGY 4 cut(s) 273, 1028, 1100, 2692
BsrI ACTGG 7 cut(s) 370, 395, 889, 1230, 1475, 2229, 2560
BssAI RCCGGY 4 cut(s) 273, 1028, 1100, 2692
BssECI CCNNGG 8 cut(s) 646, 824, 1002, 1003, 1704, 2107, 2197, 2786
BssMI GATC 9 cut(s) 158, 347, 499, 586, 718, 1519, 2448, 2538, 2782
BssNAI GTATAC 1 cut(s) 2647
BssNI GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
BssT1I CCWWGG 4 cut(s) 824, 1704, 2107, 2786
Bst1107I GTATAC 1 cut(s) 2647
Bst2UI CCWGG 3 cut(s) 606, 647, 1004
Bst4CI ACNGT 5 cut(s) 596, 1343, 1571, 2651, 2864
Bst6I CTCTTC 6 cut(s) 944, 1435, 1712, 2077, 2630, 2947
BstACI GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
BstBAI YACGTR 1 cut(s) 2509
BstBI TTCGAA 2 cut(s) 666, 2499
BstC8I GCNNGC 7 cut(s) 1356, 1370, 1925, 2262, 2526, 2581, 2793
BstDEI CTNAG 9 cut(s) 385, 488, 632, 811, 1139, 1451, 1523, 2604, 2621
BstDSI CCRYGG 2 cut(s) 2107, 2197
BstF5I GGATG 5 cut(s) 896, 1020, 1127, 1625, 2555
BstFNI CGCG 2 cut(s) 980, 2919
BstHHI GCGC 2 cut(s) 2602, 2921
BstKTI GATC 9 cut(s) 161, 350, 502, 589, 721, 1522, 2451, 2541, 2785
BstMAI GTCTC 4 cut(s) 156, 239, 628, 2454
BstMBI GATC 9 cut(s) 158, 347, 499, 586, 718, 1519, 2448, 2538, 2782
BstMCI CGRYCG 2 cut(s) 2693, 2697
BstMWI GCNNNNNNNGC 5 cut(s) 1072, 1955, 2402, 2510, 2589
BstNI CCWGG 3 cut(s) 606, 647, 1004
BstNSI RCATGY 3 cut(s) 1669, 2342, 2569
BstPAI GACNNNNGTC 2 cut(s) 1217, 1596
BstSCI CCNGG 5 cut(s) 604, 645, 1002, 1775, 1879
BstSFI CTRYAG 3 cut(s) 1067, 1488, 1953
BstSLI GKGCMC 2 cut(s) 1994, 2808
BstUI CGCG 2 cut(s) 980, 2919
BstV1I GCAGC 8 cut(s) 351, 1053, 1059, 1081, 1936, 1939, 1967, 2392
BstX2I RGATCY 2 cut(s) 499, 718
BstXI CCANNNNNNTGG 3 cut(s) 377, 1705, 2001
BstYI RGATCY 2 cut(s) 499, 718
BstZ17I GTATAC 1 cut(s) 2647
BstZI CGGCCG 1 cut(s) 2690
BsuI GTATCC 1 cut(s) 1137
BsuRI GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2692, 2795, 2875
BtgI CCRYGG 2 cut(s) 2107, 2197
BtrI CACGTC 1 cut(s) 2047
BtsCI GGATG 5 cut(s) 896, 1020, 1127, 1625, 2555
BtsI GCAGTG 2 cut(s) 219, 2145
BtsIMutI CAGTG 9 cut(s) 79, 219, 382, 1459, 2145, 2222, 2567, 2618, 2869
Cac8I GCNNGC 7 cut(s) 1356, 1370, 1925, 2262, 2526, 2581, 2793
CciI TCATGA 2 cut(s) 754, 942
CfoI GCGC 2 cut(s) 2602, 2921
Cfr10I RCCGGY 4 cut(s) 273, 1028, 1100, 2692
Cfr13I GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
CseI GACGC 3 cut(s) 2675, 2704, 2774
Csp6I GTAC 7 cut(s) 493, 565, 1508, 2305, 2386, 2614, 2852
CspAI ACCGGT 1 cut(s) 1100
CviQI GTAC 7 cut(s) 493, 565, 1508, 2305, 2386, 2614, 2852
DdeI CTNAG 9 cut(s) 385, 488, 632, 811, 1139, 1451, 1523, 2604, 2621
DpnI GATC 9 cut(s) 160, 349, 501, 588, 720, 1521, 2450, 2540, 2784
DpnII GATC 9 cut(s) 158, 347, 499, 586, 718, 1519, 2448, 2538, 2782
DraI TTTAAA 1 cut(s) 3007
DraIII CACNNNGTG 1 cut(s) 1454
DriI GACNNNNNGTC 1 cut(s) 2751
EaeI YGGCCR 3 cut(s) 1051, 2690, 2793
EagI CGGCCG 1 cut(s) 2690
Eam1104I CTCTTC 6 cut(s) 944, 1435, 1712, 2077, 2630, 2947
Eam1105I GACNNNNNGTC 1 cut(s) 2751
EarI CTCTTC 6 cut(s) 944, 1435, 1712, 2077, 2630, 2947
EciI GGCGGA 2 cut(s) 2528, 2565
EclXI CGGCCG 1 cut(s) 2690
Eco130I CCWWGG 4 cut(s) 824, 1704, 2107, 2786
Eco147I AGGCCT 1 cut(s) 267
Eco24I GRGCYC 2 cut(s) 1994, 2526
Eco47I GGWCC 3 cut(s) 1175, 1400, 1472
Eco52I CGGCCG 1 cut(s) 2690
Eco72I CACGTG 1 cut(s) 2509
Eco88I CYCGRG 2 cut(s) 1468, 1540
EcoO109I RGGNCCY 1 cut(s) 1990
EcoRI GAATTC 4 cut(s) 142, 183, 1531, 2030
EcoRII CCWGG 3 cut(s) 604, 645, 1002
EcoT14I CCWWGG 4 cut(s) 824, 1704, 2107, 2786
EcoT38I GRGCYC 2 cut(s) 1994, 2526
ErhI CCWWGG 4 cut(s) 824, 1704, 2107, 2786
Esp3I CGTCTC 2 cut(s) 156, 2454
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 1895, 1927
FaqI GGGAC 4 cut(s) 515, 760, 1210, 1463
FauI CCCGC 2 cut(s) 1119, 2351
FauNDI CATATG 1 cut(s) 1931
FblI GTMKAC 2 cut(s) 2646, 2718
FokI GGATG 5 cut(s) 883, 1027, 1114, 1612, 2542
FriOI GRGCYC 2 cut(s) 1994, 2526
FspBI CTAG 8 cut(s) 41, 195, 293, 551, 1206, 1215, 1940, 2171
GlaI GCGC 2 cut(s) 2601, 2920
GsaI CCCAGC 2 cut(s) 178, 1815
GsuI CTGGAG 1 cut(s) 385
HaeIII GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2692, 2795, 2875
HgaI GACGC 3 cut(s) 2675, 2704, 2774
HhaI GCGC 2 cut(s) 2602, 2921
Hin1I GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
Hin6I GCGC 2 cut(s) 2600, 2919
HinP1I GCGC 2 cut(s) 2600, 2919
HincII GTYRAC 3 cut(s) 24, 2344, 2719
HindII GTYRAC 3 cut(s) 24, 2344, 2719
HindIII AAGCTT 2 cut(s) 2147, 2320
HinfI GANTC 9 cut(s) 676, 703, 1218, 1597, 1772, 1816, 1997, 2202, 2933
Hpy99I CGWCG 6 cut(s) 980, 1893, 2546, 2645, 2691, 2720
HpyAV CCTTC 7 cut(s) 662, 1753, 1798, 1960, 2017, 2462, 2705
HpyCH4III ACNGT 5 cut(s) 596, 1343, 1571, 2651, 2864
HpyCH4IV ACGT 6 cut(s) 975, 1888, 2046, 2463, 2508, 2643
HpyF10VI GCNNNNNNNGC 5 cut(s) 1072, 1955, 2402, 2510, 2589
HpyF3I CTNAG 9 cut(s) 385, 488, 632, 811, 1139, 1451, 1523, 2604, 2621
HpySE526I ACGT 6 cut(s) 975, 1888, 2046, 2463, 2508, 2643
Hsp92I GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
HspAI GCGC 2 cut(s) 2600, 2919
Kpn2I TCCGGA 1 cut(s) 1555
Kzo9I GATC 9 cut(s) 158, 347, 499, 586, 718, 1519, 2448, 2538, 2782
LguI GCTCTTC 2 cut(s) 1712, 2630
LmnI GCTCC 7 cut(s) 457, 1940, 2090, 2179, 2327, 2444, 2552
Lsp1109I GCAGC 8 cut(s) 351, 1053, 1059, 1081, 1936, 1939, 1967, 2392
LweI GCATC 2 cut(s) 179, 2564
MaeI CTAG 8 cut(s) 41, 195, 293, 551, 1206, 1215, 1940, 2171
MaeII ACGT 6 cut(s) 975, 1888, 2046, 2463, 2508, 2643
MaeIII GTNAC 1 cut(s) 2743
MalI GATC 9 cut(s) 160, 349, 501, 588, 720, 1521, 2450, 2540, 2784
MbiI CCGCTC 1 cut(s) 2330
MboI GATC 9 cut(s) 158, 347, 499, 586, 718, 1519, 2448, 2538, 2782
MfeI CAATTG 3 cut(s) 2397, 2574, 2749
MflI RGATCY 2 cut(s) 499, 718
MhlI GDGCHC 3 cut(s) 1994, 2526, 2808
MlsI TGGCCA 1 cut(s) 2795
MluNI TGGCCA 1 cut(s) 2795
MlyI GAGTC 3 cut(s) 1227, 1606, 2927
Mox20I TGGCCA 1 cut(s) 2795
MroI TCCGGA 1 cut(s) 1555
MroXI GAANNNNTTC 2 cut(s) 1022, 2892
MscI TGGCCA 1 cut(s) 2795
MslI CAYNNNNRTG 4 cut(s) 1664, 2560, 2801, 2840
Msp20I TGGCCA 1 cut(s) 2795
MspA1I CMGCKG 2 cut(s) 174, 1072
MspR9I CCNGG 5 cut(s) 606, 647, 1004, 1777, 1881
MunI CAATTG 3 cut(s) 2397, 2574, 2749
Mva1269I GAATGC 3 cut(s) 1251, 1339, 2060
MvaI CCWGG 3 cut(s) 606, 647, 1004
MvnI CGCG 2 cut(s) 980, 2919
MwoI GCNNNNNNNGC 5 cut(s) 1072, 1955, 2402, 2510, 2589
NciI CCSGG 2 cut(s) 1777, 1881
NcoI CCATGG 1 cut(s) 2107
NdeI CATATG 1 cut(s) 1931
NdeII GATC 9 cut(s) 158, 347, 499, 586, 718, 1519, 2448, 2538, 2782
NlaIV GGNNCC 7 cut(s) 9, 46, 1321, 1473, 1711, 1834, 1992
NmeAIII GCCGAG 1 cut(s) 1029
NmuCI GTSAC 1 cut(s) 2743
NspI RCATGY 3 cut(s) 1669, 2342, 2569
NspV TTCGAA 2 cut(s) 666, 2499
PagI TCATGA 2 cut(s) 754, 942
PasI CCCWGGG 1 cut(s) 1003
PceI AGGCCT 1 cut(s) 267
PciI ACATGT 1 cut(s) 1665
PciSI GCTCTTC 2 cut(s) 1712, 2630
PcsI WCGNNNNNNNCGW 2 cut(s) 2505, 2637
PctI GAATGC 3 cut(s) 1251, 1339, 2060
PdmI GAANNNNTTC 2 cut(s) 1022, 2892
PfeI GAWTC 6 cut(s) 676, 703, 1772, 1816, 1997, 2202
PflFI GACNNNGTC 2 cut(s) 974, 2723
PflMI CCANNNNNTGG 1 cut(s) 2224
PinAI ACCGGT 1 cut(s) 1100
PleI GAGTC 3 cut(s) 1226, 1605, 2927
PmaCI CACGTG 1 cut(s) 2509
PmlI CACGTG 1 cut(s) 2509
PpsI GAGTC 3 cut(s) 1226, 1605, 2927
Ppu21I YACGTR 1 cut(s) 2509
PscI ACATGT 1 cut(s) 1665
PshAI GACNNNNGTC 2 cut(s) 1217, 1596
Psp6I CCWGG 3 cut(s) 604, 645, 1002
PspCI CACGTG 1 cut(s) 2509
PspFI CCCAGC 2 cut(s) 174, 1811
PspGI CCWGG 3 cut(s) 604, 645, 1002
PspN4I GGNNCC 7 cut(s) 9, 46, 1321, 1473, 1711, 1834, 1992
PspOMI GGGCCC 1 cut(s) 1990
PspPI GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
PstI CTGCAG 2 cut(s) 1071, 1957
PsuI RGATCY 2 cut(s) 499, 718
PsyI GACNNNGTC 2 cut(s) 974, 2723
PvuII CAGCTG 2 cut(s) 174, 1072
RsaI GTAC 7 cut(s) 494, 566, 1509, 2306, 2387, 2615, 2853
RsaNI GTAC 7 cut(s) 493, 565, 1508, 2305, 2386, 2614, 2852
RseI CAYNNNNRTG 4 cut(s) 1664, 2560, 2801, 2840
SalI GTCGAC 1 cut(s) 2717
SapI GCTCTTC 2 cut(s) 1712, 2630
Sau3AI GATC 9 cut(s) 158, 347, 499, 586, 718, 1519, 2448, 2538, 2782
Sau96I GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
ScaI AGTACT 2 cut(s) 566, 1509
SchI GAGTC 3 cut(s) 1227, 1606, 2927
ScrFI CCNGG 5 cut(s) 606, 647, 1004, 1777, 1881
SduI GDGCHC 3 cut(s) 1994, 2526, 2808
SfaNI GCATC 2 cut(s) 179, 2564
SfcI CTRYAG 3 cut(s) 1067, 1488, 1953
SfuI TTCGAA 2 cut(s) 666, 2499
SinI GGWCC 3 cut(s) 1175, 1400, 1472
SmiMI CAYNNNNRTG 4 cut(s) 1664, 2560, 2801, 2840
SmlI CTYRAG 3 cut(s) 98, 923, 2368
SmoI CTYRAG 3 cut(s) 98, 923, 2368
SpeI ACTAGT 1 cut(s) 550
SseBI AGGCCT 1 cut(s) 267
SsiI CCGC 9 cut(s) 980, 1112, 1830, 2330, 2358, 2513, 2547, 2550, 2769
SspI AATATT 1 cut(s) 2479
SspMI CTAG 8 cut(s) 41, 195, 293, 551, 1206, 1215, 1940, 2171
StuI AGGCCT 1 cut(s) 267
StyD4I CCNGG 5 cut(s) 604, 645, 1002, 1775, 1879
StyI CCWWGG 4 cut(s) 824, 1704, 2107, 2786
TaaI ACNGT 5 cut(s) 596, 1343, 1571, 2651, 2864
TaiI ACGT 6 cut(s) 978, 1891, 2049, 2466, 2511, 2646
TaqI TCGA 8 cut(s) 187, 666, 861, 1077, 1415, 2499, 2541, 2718
TatI WGTACW 3 cut(s) 564, 1507, 2304
TauI GCSGC 3 cut(s) 2333, 2550, 2771
TfiI GAWTC 6 cut(s) 676, 703, 1772, 1816, 1997, 2202
TscAI CASTG 9 cut(s) 79, 219, 382, 1459, 2145, 2229, 2567, 2625, 2869
TseFI GTSAC 1 cut(s) 2743
TseI GCWGC 8 cut(s) 339, 1066, 1069, 1072, 1949, 1952, 1955, 2405
Tsp45I GTSAC 1 cut(s) 2743
TspGWI ACGGA 2 cut(s) 756, 2214
TspRI CASTG 9 cut(s) 79, 219, 382, 1459, 2145, 2229, 2567, 2625, 2869
Tth111I GACNNNGTC 2 cut(s) 974, 2723
Van91I CCANNNNNTGG 1 cut(s) 2224
VpaK11BI GGWCC 3 cut(s) 1175, 1400, 1472
XbaI TCTAGA 1 cut(s) 194
XceI RCATGY 3 cut(s) 1669, 2342, 2569
XmiI GTMKAC 2 cut(s) 2646, 2718
XmnI GAANNNNTTC 2 cut(s) 1022, 2892
XspI CTAG 8 cut(s) 41, 195, 293, 551, 1206, 1215, 1940, 2171
ZraI GACGTC 1 cut(s) 2464
ZrmI AGTACT 2 cut(s) 566, 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.