RLG00000002210

leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010572
Physical Location & Seq
Reverse (-)
29639350 .. 29643370
4021 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002210

Sequence Viewer

Length: 3027 bp
ATGGTGGTGCCTTTCATTTTCTTGACACTTTTCTCTGTTCTAGGAACCCCCTGTTTTGCTGCTTCTTTGGTTAGTGATTTTCATGTCTTACTCGCACTCAAGCAAGGCTTCAAATTCTCCGAACCAGCTTTAAGTAGTTGGAATTCTTCGTCTCCAAGATCAGTTTGTTCCAGCTGGGTTGGAATTCGATGCTCTAGAGGACGAGTTGTTGCAGTGGACTTGACAGGTTTCAGTCTCGCTGGTTCTGTTTCGCCTATCATTTCAGGCCTTGACCGGCTTTCCGACCTCTCTCTAGCCGGAAACAACTTGTCAGGAAGCATTGAGATTGCAAATTTGGGCAGCCTCCGATTTCTCAACATCTCCGACAACCAGTTCAGTGGGGGCTTAGACTGGAACTACTCCAGCATTGCCAACTTGGAGGTTTTTGATGTCTATGATAACAACTTGACTACTCCACTTCCACTTGGGATTTTGAGCTTGAATAAGCTCAGGTACTTGGATCTCGGAGGCAACTTCTTCTATGGGAAAGTCCCACCATTATACGGCAAACTAGTCAGCTTGGAGTACTTGTCACTTTCAGGCAATGATCTTAACGGTGAAATTCCAGGTGCGTTGGGCAATCTCACTAACCTGAGACAACTTTACTTGGGCTACTACAATGTTTTCGAAGGTGGGATTCCGAAGCAGTTTGGGAAATTGGTGAATCTTGTTCACATGGATCTTTCAAGTTGCGAATTGGACGGACAAATCCCTCATGAGTTGGGGAATTTGAAGTCCCTTGACACGCTTTATTTGCATATCAATCTGCTCTCAGGCTCAATTCCAAGGCAGTTGGGCAACTTGACAAACTTGCTGTATTTCGACCTCTCCAACAATGCACTTACTGGTGAAATTCCATCCGAGTTTTCAAATCTCAAACAGCTCAAGCTCTTTAACCTCTTCATGAACAGATTGCACGGGTCTATTCCAGACTACGTCGCGGATTTGCCCGATTTGGACACCCTGGGGCTGTGGATGAACAACTTCACCGGCATAATTCCTCAGAAACTTGGCCAAAATGGGAAGCTGCAGCTGCTCGATTTGTCCTCAAATAAGCTCACCGGTAAAATCCCGCCAAACTTGTGTTCATCCAATCAGCTAAGGATACTAATTCTGTTAAAAAACTTTCTGTTTGGACCAATCCCTTTAGGCTTGGGGACTTGTTCTAGTCTGACTAGAGTCAGACTGGGGCAGAATTACCTGAATGGTAGCATTCCAAATGGCTTAATTTACTTGCCTCTGCTCAATTTAGCAGAGTTGCAAGATAATTATCTTTCCGGCACCTTGTCGGAGAATGCCAATAGTTCATCACAGCCTGCCCTATTAGGCCAGCTTAATCTGTCAAACAATCTCTTATCCGGTCCTATACCATATTCGATTTCAAATTTCTCTTCCCTCCAAATCCTCTCACTCAGTGGAAATCAGCTCTCGGGTCCAATCCCACCATCTATAGGCCAACTCCATCAAGTACTAAACCTTGATCTTAGCCGGAATTCACTCTCGGGTGAAGTTCCTCCGGAAATTGGAAACTGTTTCCATCTGACTTACCTTGACATGAGTCAGAACAACCTCTCTGGCTCAATCCCATCCGAAATCTCCAACATTCATATACTGAATTACATCAACATGTCAAGAAACCACTTGAACCAGAGCATACCCAAATCCTTGGGAACCATGAAGAGCCTCACAACTGCTGATTTTTCCTTCAATGACTTGTCGGGTAAACTACCCGAATCCGGGCAGTTTGCCTTCTTCAATGCTTCCTCTTTCGCTGGGAATCCCCATCTTTGCGGTTCCCTCCTAAACAATCCTTGCAATTTCACCACAATCACAAACACACCCGGAAAACCTCGGGCAGATATCAAGCTAATCTTCGCATTAGGCTTGCTCATATGCTCCCTAGTATTTGCTGCTGCAGCCATAATGAAGGCCAAATCGTTCACAAGAAAGGGCCCAGAATCATGGAAAATGACAGCATTTCAGAAGGTGGAATTCACAATGTCGGACGTGCTGGAATGCGTGAAAGATGGAAATGTGATTGGAAGAGGAGGAGCAGGAATTGTTTACCATGGGAAAATGCCAAACGGGGTTGAAATTGCAGTGAAAAAGCTTCTTGGTTTTGGGCTTGGTCTGGCTCCGAACAGCACCCATGACGACCACGGATTCAGAGCCGAAATCCAAACACTGGGCAACATTCGTCATAGAAACATTGTGAGATTGCTGGCATTTTGCTCAAACAAGGACACCAATCTGCTTGTTTACGACTACATGAGAAATGGAAGCTTGGGAGAGGCATTGCATGTCAACAACAAAAAAGGCGGGTTATTCTTGAGTTGGAATTTGAGGTACAAAATTGCAATTGAAGCTGCCAAAGGGTTGTGCTATCTCCACCACGACTGCTCCCCATTGATCCTTCACCGAGACGTCAAATCCAACAATATTTTGCTCAATTCCAGCTTCGAAGCACACGTGGCCGATTTCGGGCTCGCCAAGTTCTTGATAGACGGCGGCGGAGCATCCCAGTGCATGTCAGCAATTGCAGGCTCTTACGGCTACATTGCGCCTGAGTATGCGTACACACTGAGAGTGGACGAGAAGAGCGACGTATATAGTTTCGGTGTTGTCTTGTTAGAGCTTCTAACAGGGCGTCGCCCGGTCGGTGAGTTTGGCGAAGGCGTCGACATAGTCCAATGGTGTAAAAAAGTCACCAATTGTCGCAAACAAGACGCCGCAAACATTGTTGATCCAAGGTTGGCCATATCTGTGCCCAAAGATGAAGCAATGCACTTGTTTTTCATTGCAATGCTTTGCGTACAAGAAAACAGTGTTGAGAGGCCAACAATGAGAGAAGTTGTTCAAATGTTGTCAGAATTTCCTCGCGCCCATTCTCCCGACTCCTCTTCTTCTTTAATCACTTCCCAACAATTCAATAACAAACAACAAGACGGTAAATGTCCTCCTAAATTTAAACAACATATTTTAGTTTAA

Protein Analysis

1009

Amino Acids

109.94

Weight (kDa)

7.53

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 65 7e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 216 9.1e-09 Leucine-rich repeat region
LRR_14 PF23598 228 - 335 1.9e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 690 - 968 1.4e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 690 - 966 3.9e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2466
AccB1I GGYRCC 2 cut(s) 7, 1319
AccB7I CCANNNNNTGG 1 cut(s) 2224
AccI GTMKAC 1 cut(s) 2718
AccII CGCG 2 cut(s) 980, 2919
AccIII TCCGGA 1 cut(s) 1555
AciI CCGC 7 cut(s) 980, 1112, 1830, 2358, 2547, 2550, 2769
AclWI GGATC 4 cut(s) 507, 726, 2443, 2777
AcoI YGGCCR 3 cut(s) 1051, 2511, 2793
AcvI CACGTG 1 cut(s) 2509
AcyI GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
AdeI CACNNNGTG 1 cut(s) 1454
AfaI GTAC 6 cut(s) 494, 566, 1509, 2387, 2615, 2853
AfiI CCNNNNNNNGG 7 cut(s) 542, 1490, 1562, 1775, 1776, 2224, 2520
AflIII ACRYGT 2 cut(s) 1665, 2506
AgeI ACCGGT 1 cut(s) 1100
AhlI ACTAGT 1 cut(s) 550
AjiI CACGTC 1 cut(s) 2047
AjnI CCWGG 2 cut(s) 604, 1002
AjuI GAANNNNNNNTTGG 2 cut(s) 1108, 1140
Alw26I GTCTC 4 cut(s) 156, 239, 628, 2454
AlwI GGATC 4 cut(s) 507, 726, 2443, 2777
Ama87I CYCGRG 3 cut(s) 1468, 1540, 1890
Aor13HI TCCGGA 1 cut(s) 1555
AoxI GGCC 9 cut(s) 265, 1051, 1366, 1492, 1968, 1990, 2511, 2793, 2873
ApaI GGGCCC 1 cut(s) 1994
ApeKI GCWGC 9 cut(s) 59, 339, 1066, 1069, 1072, 1949, 1952, 1955, 2405
AsiGI ACCGGT 1 cut(s) 1100
Asp700I GAANNNNTTC 2 cut(s) 1022, 2892
AspLEI GCGC 2 cut(s) 2602, 2921
AspS9I GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
AsuC2I CCSGG 3 cut(s) 1777, 1881, 2693
AsuII TTCGAA 2 cut(s) 666, 2499
AvaI CYCGRG 3 cut(s) 1468, 1540, 1890
AvaII GGWCC 3 cut(s) 1175, 1400, 1472
BaeGI GKGCMC 2 cut(s) 1994, 2808
BalI TGGCCA 2 cut(s) 1053, 2795
BanI GGYRCC 2 cut(s) 7, 1319
BanII GRGCYC 2 cut(s) 1994, 2526
BbrPI CACGTG 1 cut(s) 2509
BbvI GCAGC 9 cut(s) 46, 351, 1053, 1059, 1081, 1936, 1939, 1967, 2392
BccI CCATC 7 cut(s) 904, 1492, 1509, 1584, 1633, 1830, 2060
BceAI ACGGC 3 cut(s) 559, 2560, 2605
BciT130I CCWGG 2 cut(s) 606, 1004
BciVI GTATCC 1 cut(s) 1137
BcnI CCSGG 3 cut(s) 1777, 1881, 2693
BcoDI GTCTC 4 cut(s) 156, 239, 628, 2454
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 7 cut(s) 41, 195, 293, 551, 1206, 1215, 1940
BfmI CTRYAG 3 cut(s) 1067, 1488, 1953
BfuI GTATCC 1 cut(s) 1137
BmcAI AGTACT 2 cut(s) 566, 1509
Bme1390I CCNGG 5 cut(s) 606, 1004, 1777, 1881, 2693
Bme18I GGWCC 3 cut(s) 1175, 1400, 1472
BmeT110I CYCGRG 3 cut(s) 1468, 1540, 1890
BmgBI CACGTC 1 cut(s) 2047
BmgT120I GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
BmiI GGNNCC 8 cut(s) 9, 46, 1321, 1473, 1711, 1834, 1992, 2175
BmrFI CCNGG 5 cut(s) 606, 1004, 1777, 1881, 2693
BmrI ACTGGG 3 cut(s) 1235, 2234, 2554
BmsI GCATC 2 cut(s) 179, 2564
BmuI ACTGGG 3 cut(s) 1235, 2234, 2554
BoxI GACNNNNGTC 2 cut(s) 1217, 1596
BpmI CTGGAG 1 cut(s) 385
Bpu10I CCTNAGC 2 cut(s) 488, 1139
Bpu14I TTCGAA 2 cut(s) 666, 2499
BpuEI CTTGAG 3 cut(s) 83, 908, 2389
BpuMI CCSGG 3 cut(s) 1777, 1881, 2693
BsaAI YACGTR 1 cut(s) 2509
BsaBI GATNNNNATC 1 cut(s) 1308
BsaHI GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
BsaJI CCNNGG 8 cut(s) 824, 1002, 1003, 1704, 1889, 2107, 2197, 2786
BsaWI WCCGGW 3 cut(s) 1100, 1397, 1555
BsaXI ACNNNNNCTCC 4 cut(s) 554, 584, 2423, 2453
Bsc4I CCNNNNNNNGG 7 cut(s) 542, 1490, 1562, 1775, 1776, 2224, 2520
Bse118I RCCGGY 3 cut(s) 273, 1028, 1100
Bse1I ACTGG 6 cut(s) 370, 395, 889, 1230, 2229, 2560
Bse3DI GCAATG 7 cut(s) 405, 589, 2333, 2595, 2826, 2835, 2847
Bse8I GATNNNNATC 1 cut(s) 1308
BseAI TCCGGA 1 cut(s) 1555
BseBI CCWGG 2 cut(s) 606, 1004
BseDI CCNNGG 8 cut(s) 824, 1002, 1003, 1704, 1889, 2107, 2197, 2786
BseGI GGATG 5 cut(s) 896, 1020, 1127, 1625, 2555
BseJI GATNNNNATC 1 cut(s) 1308
BseLI CCNNNNNNNGG 7 cut(s) 542, 1490, 1562, 1775, 1776, 2224, 2520
BseMI GCAATG 7 cut(s) 405, 589, 2333, 2595, 2826, 2835, 2847
BseMII CTCAG 7 cut(s) 502, 623, 825, 1055, 1465, 2595, 2612
BseNI ACTGG 6 cut(s) 370, 395, 889, 1230, 2229, 2560
BseRI GAGGAG 3 cut(s) 2100, 2103, 2926
BseSI GKGCMC 2 cut(s) 1994, 2808
BseXI GCAGC 9 cut(s) 46, 351, 1053, 1059, 1081, 1936, 1939, 1967, 2392
BseYI CCCAGC 2 cut(s) 174, 1811
Bsh1236I CGCG 2 cut(s) 980, 2919
Bsh1285I CGRYCG 1 cut(s) 2697
BshFI GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2513, 2795, 2875
BshNI GGYRCC 2 cut(s) 7, 1319
BshTI ACCGGT 1 cut(s) 1100
BsiEI CGRYCG 1 cut(s) 2697
BsiHKCI CYCGRG 3 cut(s) 1468, 1540, 1890
BslFI GGGAC 3 cut(s) 515, 760, 1210
BslI CCNNNNNNNGG 7 cut(s) 542, 1490, 1562, 1775, 1776, 2224, 2520
BsmAI GTCTC 4 cut(s) 156, 239, 628, 2454
BsmBI CGTCTC 2 cut(s) 156, 2454
BsmFI GGGAC 3 cut(s) 515, 760, 1210
BsmI GAATGC 3 cut(s) 1251, 1339, 2060
BsnI GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2513, 2795, 2875
BsoBI CYCGRG 3 cut(s) 1468, 1540, 1890
Bsp119I TTCGAA 2 cut(s) 666, 2499
Bsp120I GGGCCC 1 cut(s) 1990
Bsp1286I GDGCHC 3 cut(s) 1994, 2526, 2808
Bsp13I TCCGGA 1 cut(s) 1555
Bsp143I GATC 7 cut(s) 158, 499, 586, 718, 1519, 2448, 2782
Bsp19I CCATGG 1 cut(s) 2107
BspACI CCGC 7 cut(s) 980, 1112, 1830, 2358, 2547, 2550, 2769
BspANI GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2513, 2795, 2875
BspCNI CTCAG 7 cut(s) 501, 624, 824, 1054, 1464, 2596, 2613
BspEI TCCGGA 1 cut(s) 1555
BspFNI CGCG 2 cut(s) 980, 2919
BspHI TCATGA 2 cut(s) 754, 942
BspLI GGNNCC 8 cut(s) 9, 46, 1321, 1473, 1711, 1834, 1992, 2175
BspMAI CTGCAG 2 cut(s) 1071, 1957
BspPI GGATC 4 cut(s) 507, 726, 2443, 2777
BspQI GCTCTTC 2 cut(s) 1712, 2630
BspT104I TTCGAA 2 cut(s) 666, 2499
BspT107I GGYRCC 2 cut(s) 7, 1319
BsrDI GCAATG 7 cut(s) 405, 589, 2333, 2595, 2826, 2835, 2847
BsrFI RCCGGY 3 cut(s) 273, 1028, 1100
BsrI ACTGG 6 cut(s) 370, 395, 889, 1230, 2229, 2560
BssAI RCCGGY 3 cut(s) 273, 1028, 1100
BssECI CCNNGG 8 cut(s) 824, 1002, 1003, 1704, 1889, 2107, 2197, 2786
BssMI GATC 7 cut(s) 158, 499, 586, 718, 1519, 2448, 2782
BssNI GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
BssT1I CCWWGG 4 cut(s) 824, 1704, 2107, 2786
Bst2UI CCWGG 2 cut(s) 606, 1004
Bst4CI ACNGT 4 cut(s) 596, 1571, 2864, 2987
Bst6I CTCTTC 6 cut(s) 944, 1435, 1712, 2077, 2630, 2944
BstACI GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
BstBAI YACGTR 1 cut(s) 2509
BstBI TTCGAA 2 cut(s) 666, 2499
BstC8I GCNNGC 6 cut(s) 1356, 1370, 1925, 2262, 2526, 2581
BstDSI CCRYGG 2 cut(s) 2107, 2197
BstF5I GGATG 5 cut(s) 896, 1020, 1127, 1625, 2555
BstFNI CGCG 2 cut(s) 980, 2919
BstHHI GCGC 2 cut(s) 2602, 2921
BstKTI GATC 7 cut(s) 161, 502, 589, 721, 1522, 2451, 2785
BstMAI GTCTC 4 cut(s) 156, 239, 628, 2454
BstMBI GATC 7 cut(s) 158, 499, 586, 718, 1519, 2448, 2782
BstMCI CGRYCG 1 cut(s) 2697
BstMWI GCNNNNNNNGC 6 cut(s) 793, 1072, 1955, 2402, 2510, 2589
BstNI CCWGG 2 cut(s) 606, 1004
BstNSI RCATGY 3 cut(s) 1669, 2342, 2569
BstPAI GACNNNNGTC 2 cut(s) 1217, 1596
BstSCI CCNGG 5 cut(s) 604, 1002, 1775, 1879, 2691
BstSFI CTRYAG 3 cut(s) 1067, 1488, 1953
BstSLI GKGCMC 2 cut(s) 1994, 2808
BstUI CGCG 2 cut(s) 980, 2919
BstV1I GCAGC 9 cut(s) 46, 351, 1053, 1059, 1081, 1936, 1939, 1967, 2392
BstX2I RGATCY 2 cut(s) 499, 718
BstXI CCANNNNNNTGG 3 cut(s) 377, 1705, 2001
BstYI RGATCY 2 cut(s) 499, 718
BsuI GTATCC 1 cut(s) 1137
BsuRI GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2513, 2795, 2875
BtgI CCRYGG 2 cut(s) 2107, 2197
BtrI CACGTC 1 cut(s) 2047
BtsCI GGATG 5 cut(s) 896, 1020, 1127, 1625, 2555
BtsI GCAGTG 2 cut(s) 219, 2145
BtsIMutI CAGTG 8 cut(s) 219, 382, 1459, 2145, 2222, 2567, 2618, 2869
Cac8I GCNNGC 6 cut(s) 1356, 1370, 1925, 2262, 2526, 2581
CciI TCATGA 2 cut(s) 754, 942
CfoI GCGC 2 cut(s) 2602, 2921
Cfr10I RCCGGY 3 cut(s) 273, 1028, 1100
Cfr13I GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
CseI GACGC 3 cut(s) 2675, 2704, 2774
Csp6I GTAC 6 cut(s) 493, 565, 1508, 2386, 2614, 2852
CspAI ACCGGT 1 cut(s) 1100
CviQI GTAC 6 cut(s) 493, 565, 1508, 2386, 2614, 2852
DpnI GATC 7 cut(s) 160, 501, 588, 720, 1521, 2450, 2784
DpnII GATC 7 cut(s) 158, 499, 586, 718, 1519, 2448, 2782
DraI TTTAAA 1 cut(s) 3007
DraIII CACNNNGTG 1 cut(s) 1454
EaeI YGGCCR 3 cut(s) 1051, 2511, 2793
Eam1104I CTCTTC 6 cut(s) 944, 1435, 1712, 2077, 2630, 2944
EarI CTCTTC 6 cut(s) 944, 1435, 1712, 2077, 2630, 2944
EciI GGCGGA 1 cut(s) 2565
Eco130I CCWWGG 4 cut(s) 824, 1704, 2107, 2786
Eco147I AGGCCT 1 cut(s) 267
Eco24I GRGCYC 2 cut(s) 1994, 2526
Eco32I GATATC 1 cut(s) 1900
Eco47I GGWCC 3 cut(s) 1175, 1400, 1472
Eco72I CACGTG 1 cut(s) 2509
Eco88I CYCGRG 3 cut(s) 1468, 1540, 1890
EcoO109I RGGNCCY 1 cut(s) 1990
EcoRI GAATTC 4 cut(s) 142, 183, 1531, 2030
EcoRII CCWGG 2 cut(s) 604, 1002
EcoRV GATATC 1 cut(s) 1900
EcoT14I CCWWGG 4 cut(s) 824, 1704, 2107, 2786
EcoT38I GRGCYC 2 cut(s) 1994, 2526
ErhI CCWWGG 4 cut(s) 824, 1704, 2107, 2786
Esp3I CGTCTC 2 cut(s) 156, 2454
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 1895, 1927
FaqI GGGAC 3 cut(s) 515, 760, 1210
FauI CCCGC 2 cut(s) 1119, 2351
FauNDI CATATG 1 cut(s) 1931
FblI GTMKAC 1 cut(s) 2718
FokI GGATG 5 cut(s) 883, 1027, 1114, 1612, 2542
FriOI GRGCYC 2 cut(s) 1994, 2526
FspBI CTAG 7 cut(s) 41, 195, 293, 551, 1206, 1215, 1940
GlaI GCGC 2 cut(s) 2601, 2920
GsaI CCCAGC 2 cut(s) 178, 1815
GsuI CTGGAG 1 cut(s) 385
HaeIII GGCC 9 cut(s) 267, 1053, 1368, 1494, 1970, 1992, 2513, 2795, 2875
HgaI GACGC 3 cut(s) 2675, 2704, 2774
HhaI GCGC 2 cut(s) 2602, 2921
Hin1I GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
Hin6I GCGC 2 cut(s) 2600, 2919
HinP1I GCGC 2 cut(s) 2600, 2919
HincII GTYRAC 2 cut(s) 2344, 2719
HindII GTYRAC 2 cut(s) 2344, 2719
HindIII AAGCTT 2 cut(s) 2147, 2320
HinfI GANTC 9 cut(s) 676, 703, 1218, 1597, 1772, 1816, 1997, 2202, 2933
Hpy99I CGWCG 4 cut(s) 980, 2645, 2691, 2720
HpyAV CCTTC 7 cut(s) 662, 1753, 1798, 1960, 2017, 2462, 2705
HpyCH4III ACNGT 4 cut(s) 596, 1571, 2864, 2987
HpyCH4IV ACGT 5 cut(s) 975, 2046, 2463, 2508, 2643
HpyF10VI GCNNNNNNNGC 6 cut(s) 793, 1072, 1955, 2402, 2510, 2589
HpySE526I ACGT 5 cut(s) 975, 2046, 2463, 2508, 2643
Hsp92I GRCGYC 4 cut(s) 2463, 2686, 2715, 2766
HspAI GCGC 2 cut(s) 2600, 2919
Kpn2I TCCGGA 1 cut(s) 1555
Kzo9I GATC 7 cut(s) 158, 499, 586, 718, 1519, 2448, 2782
LguI GCTCTTC 2 cut(s) 1712, 2630
LmnI GCTCC 5 cut(s) 1940, 2090, 2179, 2444, 2552
Lsp1109I GCAGC 9 cut(s) 46, 351, 1053, 1059, 1081, 1936, 1939, 1967, 2392
LweI GCATC 2 cut(s) 179, 2564
MaeI CTAG 7 cut(s) 41, 195, 293, 551, 1206, 1215, 1940
MaeII ACGT 5 cut(s) 975, 2046, 2463, 2508, 2643
MaeIII GTNAC 2 cut(s) 570, 2743
MalI GATC 7 cut(s) 160, 501, 588, 720, 1521, 2450, 2784
MboI GATC 7 cut(s) 158, 499, 586, 718, 1519, 2448, 2782
MfeI CAATTG 3 cut(s) 2397, 2574, 2749
MflI RGATCY 2 cut(s) 499, 718
MhlI GDGCHC 3 cut(s) 1994, 2526, 2808
MlsI TGGCCA 2 cut(s) 1053, 2795
MluNI TGGCCA 2 cut(s) 1053, 2795
MlyI GAGTC 3 cut(s) 1227, 1606, 2927
Mox20I TGGCCA 2 cut(s) 1053, 2795
MroI TCCGGA 1 cut(s) 1555
MroXI GAANNNNTTC 2 cut(s) 1022, 2892
MscI TGGCCA 2 cut(s) 1053, 2795
MseI TTAA 9 cut(s) 131, 591, 933, 1157, 1265, 1374, 2948, 3006, 3025
MslI CAYNNNNRTG 4 cut(s) 1664, 2560, 2801, 2840
Msp20I TGGCCA 2 cut(s) 1053, 2795
MspA1I CMGCKG 2 cut(s) 174, 1072
MspR9I CCNGG 5 cut(s) 606, 1004, 1777, 1881, 2693
MunI CAATTG 3 cut(s) 2397, 2574, 2749
Mva1269I GAATGC 3 cut(s) 1251, 1339, 2060
MvaI CCWGG 2 cut(s) 606, 1004
MvnI CGCG 2 cut(s) 980, 2919
MwoI GCNNNNNNNGC 6 cut(s) 793, 1072, 1955, 2402, 2510, 2589
NciI CCSGG 3 cut(s) 1777, 1881, 2693
NcoI CCATGG 1 cut(s) 2107
NdeI CATATG 1 cut(s) 1931
NdeII GATC 7 cut(s) 158, 499, 586, 718, 1519, 2448, 2782
NlaIV GGNNCC 8 cut(s) 9, 46, 1321, 1473, 1711, 1834, 1992, 2175
NmuCI GTSAC 2 cut(s) 570, 2743
NspI RCATGY 3 cut(s) 1669, 2342, 2569
NspV TTCGAA 2 cut(s) 666, 2499
PagI TCATGA 2 cut(s) 754, 942
PasI CCCWGGG 1 cut(s) 1003
PceI AGGCCT 1 cut(s) 267
PciI ACATGT 1 cut(s) 1665
PciSI GCTCTTC 2 cut(s) 1712, 2630
PcsI WCGNNNNNNNCGW 2 cut(s) 2505, 2637
PctI GAATGC 3 cut(s) 1251, 1339, 2060
PdmI GAANNNNTTC 2 cut(s) 1022, 2892
PfeI GAWTC 6 cut(s) 676, 703, 1772, 1816, 1997, 2202
PflFI GACNNNGTC 2 cut(s) 974, 2723
PflMI CCANNNNNTGG 1 cut(s) 2224
PinAI ACCGGT 1 cut(s) 1100
PleI GAGTC 3 cut(s) 1226, 1605, 2927
PmaCI CACGTG 1 cut(s) 2509
PmlI CACGTG 1 cut(s) 2509
PpsI GAGTC 3 cut(s) 1226, 1605, 2927
Ppu21I YACGTR 1 cut(s) 2509
PscI ACATGT 1 cut(s) 1665
PshAI GACNNNNGTC 2 cut(s) 1217, 1596
Psp6I CCWGG 2 cut(s) 604, 1002
PspCI CACGTG 1 cut(s) 2509
PspFI CCCAGC 2 cut(s) 174, 1811
PspGI CCWGG 2 cut(s) 604, 1002
PspN4I GGNNCC 8 cut(s) 9, 46, 1321, 1473, 1711, 1834, 1992, 2175
PspOMI GGGCCC 1 cut(s) 1990
PspPI GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
PstI CTGCAG 2 cut(s) 1071, 1957
PsuI RGATCY 2 cut(s) 499, 718
PsyI GACNNNGTC 2 cut(s) 974, 2723
PvuII CAGCTG 2 cut(s) 174, 1072
RsaI GTAC 6 cut(s) 494, 566, 1509, 2387, 2615, 2853
RsaNI GTAC 6 cut(s) 493, 565, 1508, 2386, 2614, 2852
RseI CAYNNNNRTG 4 cut(s) 1664, 2560, 2801, 2840
SalI GTCGAC 1 cut(s) 2717
SapI GCTCTTC 2 cut(s) 1712, 2630
SaqAI TTAA 9 cut(s) 131, 591, 933, 1157, 1265, 1374, 2948, 3006, 3025
Sau3AI GATC 7 cut(s) 158, 499, 586, 718, 1519, 2448, 2782
Sau96I GGNCC 5 cut(s) 1175, 1400, 1472, 1990, 1991
ScaI AGTACT 2 cut(s) 566, 1509
SchI GAGTC 3 cut(s) 1227, 1606, 2927
ScrFI CCNGG 5 cut(s) 606, 1004, 1777, 1881, 2693
SduI GDGCHC 3 cut(s) 1994, 2526, 2808
SfaNI GCATC 2 cut(s) 179, 2564
SfcI CTRYAG 3 cut(s) 1067, 1488, 1953
SfuI TTCGAA 2 cut(s) 666, 2499
SinI GGWCC 3 cut(s) 1175, 1400, 1472
SmiMI CAYNNNNRTG 4 cut(s) 1664, 2560, 2801, 2840
SmlI CTYRAG 3 cut(s) 98, 923, 2368
SmoI CTYRAG 3 cut(s) 98, 923, 2368
SpeI ACTAGT 1 cut(s) 550
SseBI AGGCCT 1 cut(s) 267
SsiI CCGC 7 cut(s) 980, 1112, 1830, 2358, 2547, 2550, 2769
SspI AATATT 1 cut(s) 2479
SspMI CTAG 7 cut(s) 41, 195, 293, 551, 1206, 1215, 1940
StuI AGGCCT 1 cut(s) 267
StyD4I CCNGG 5 cut(s) 604, 1002, 1775, 1879, 2691
StyI CCWWGG 4 cut(s) 824, 1704, 2107, 2786
TaaI ACNGT 4 cut(s) 596, 1571, 2864, 2987
TaiI ACGT 5 cut(s) 978, 2049, 2466, 2511, 2646
TaqI TCGA 7 cut(s) 187, 666, 861, 1077, 1415, 2499, 2718
TatI WGTACW 2 cut(s) 564, 1507
TauI GCSGC 2 cut(s) 2550, 2771
TfiI GAWTC 6 cut(s) 676, 703, 1772, 1816, 1997, 2202
Tru1I TTAA 9 cut(s) 131, 591, 933, 1157, 1265, 1374, 2948, 3006, 3025
Tru9I TTAA 9 cut(s) 131, 591, 933, 1157, 1265, 1374, 2948, 3006, 3025
TscAI CASTG 8 cut(s) 219, 382, 1459, 2145, 2229, 2567, 2625, 2869
TseFI GTSAC 2 cut(s) 570, 2743
TseI GCWGC 9 cut(s) 59, 339, 1066, 1069, 1072, 1949, 1952, 1955, 2405
Tsp45I GTSAC 2 cut(s) 570, 2743
TspGWI ACGGA 2 cut(s) 756, 2214
TspRI CASTG 8 cut(s) 219, 382, 1459, 2145, 2229, 2567, 2625, 2869
Tth111I GACNNNGTC 2 cut(s) 974, 2723
Van91I CCANNNNNTGG 1 cut(s) 2224
VpaK11BI GGWCC 3 cut(s) 1175, 1400, 1472
XbaI TCTAGA 1 cut(s) 194
XceI RCATGY 3 cut(s) 1669, 2342, 2569
XmiI GTMKAC 1 cut(s) 2718
XmnI GAANNNNTTC 2 cut(s) 1022, 2892
XspI CTAG 7 cut(s) 41, 195, 293, 551, 1206, 1215, 1940
ZraI GACGTC 1 cut(s) 2464
ZrmI AGTACT 2 cut(s) 566, 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.