Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0013048

Physical Location & Seq

Genomic Coordinates Reverse (-)

20754621 .. 20757919

Gene Structure 3299 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g29861.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGCTCAGAGTTCCCGCCAGTTTCCGAAACCTTAGATTTCCCCATTTTCCACTCTCACGTTCTACTTCCCGCAACAACCTATCCCAATGCTTGGTGGGTTTCATGTATCACACCAAATTGAGTGCCTCATCTTACAAGTCACTGCTCTCCGATCACCTCAGAAACCATAGACTAGATGAAGCTATCCTTCTTTTCAATACAATCCCTTCTCCCGGTGCCGATATGTTTACCAAAATGATAACGGGCTATGCGCGACGGGGTAGGCTTGACGATGCCCTGAAGCTGTTCGATGAAATGCCTCAGAGAGATGTGGTCTCGTGGAATGCGATGATCAAAGGTTGTTTGGATTGTGATGATTTGGGTAAGGCTAAGGAGCTGTTTGATGAAATGCCGGAGAAGAGTGTGGTGTCGTGGACGACCATGATGAATGGGTACTTGCAGTTTGGAGAGTTTGAAATGGCAGAGTGCTTGTTTCAGAAAATGCCCGCGAGGGATGTGGCTGCGTGGAACTCCATGATTCATGGATGTTTTAGCTATGGAAGAGTAGATGATGCTAACAAGTTGTTTGAGGTGATGCCTGGGAAGAATGCCATTTCGTGGACTTCCATGATTGCTGGGCTTGACCAGAATGGGAGGAGTGAGGAAGCTTTGGTTGTTTTCGGAGAGATGGTGGCGTCTGGCGTACAAGCCACTTTGACCACATTTGGGAGCGTGGTGACAGCTTGTGCGAATTCGGGGAGTTTAGATTTAGGTGTAGAGGTTCATGGTCAGATTGTCAAGTTAGGGTACTGCTTTGATGTATTCTTAGCTGCTTCACTTATAACACTTTATGCAAACTGCAATCAAATAGAGAATTCTCACAAGGTTTTTAAGGAGAGTGTGCATAGAAATGTGGTGGTATGGACAGCTCTTATAACAGGATGTGGATCGAATTCTAAACACGATGATGCATTGAAGGTTTTTAGGGATATGATGATCTCAGGAGTCCTCCCCAATCAATCTTCTTTCACTAGTGCTCTGAATTCATGTTTGGGATTGGAACATCTCCATAGGGGAAAGGAGATACATGCCACAGCTGTTAAGTTGGGTTTTGAAACTGATGCCTTTGTGGGTAATTCTCTCATTGTCTTGTATACCAAATGTGGAAATGTAAATGATGGAGTTGCTGTATTTAAGAGAATTGGTGAGAAGAATGTTGTCTCATGGAACTCAACCATCATTGGATGCGCGCAACACGGTCGTGGAAAGTGGGCATTGGCTCTCTTTAACCAAATGTTACGGGCAGATGTAGGCCCCGATGAAATTACATTGACTGGGTTGCTCTCTGCATGTAGCCATTCTGGAATGTTGCAGAAAGCAAGAGGCTTTTTCAAAGTTTTATCTCAGACTAAATATATTGAAATGAAAAAGGAGCACTTTACATGTATGGTGGATGTCTTGGGACGATTTGGCGAATTAGAAGAAGCGGAGGAGTTTATTAGAAACATGCCTGTTCAAGAAACTAAGATGGCTTGGCTAACTTTACTTGGTGCTTGTAGGTTGCATTCCAATTTGGATGTGGCTGAAAGAGCAGCAAAGAACATTTTTGATATAGAACCGAACTGTAGTGCTGCGTGTGTTTTATTGTCGAATATATATGCTTCTGCAAATAGATGGAGCGATGTAAGCAGAATACGAGAGAAGATGAAGTATGGTGGAACTGTAAAACAACCGGGTTCGAGTTGGTTTACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

578

Amino Acids

64.01

Weight (kDa)

7.53

Isoelectric Point (pI)

39.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR	PF01535	75 - 103	5.8e-07	PPR repeat
PPR_2	PF13041	75 - 103	6.5e-06	PPR repeat family
PPR_1	PF12854	76 - 99	4e-06	PPR repeat
PPR_2	PF13041	102 - 135	4.9e-07	PPR repeat family
PPR	PF01535	105 - 134	1.4e-06	PPR repeat
PPR_2	PF13041	134 - 166	4.4e-06	PPR repeat family
PPR	PF01535	136 - 165	1.5e-06	PPR repeat
PPR_2	PF13041	164 - 195	6.5e-06	PPR repeat family
PPR_2	PF13041	196 - 244	2.1e-07	PPR repeat family
PPR_2	PF13041	296 - 343	3.9e-10	PPR repeat family
PPR	PF01535	299 - 329	7e-06	PPR repeat
PPR_2	PF13041	397 - 445	2.9e-09	PPR repeat family
E_motif	PF20431	515 - 575	1.7e-17	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013048)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46460
fragaria_vesca	FvH4_5g29861 FvH4_5g29861
malus_domestica	MD05G1292000.v1.1
prunus_persica	Prupe.4G073400_v2.0.a1
pyrus_communis	pycom05g26840 pycom10g22400
rosa_chinensis	RchiOBHm_Chr5g0012881
rosa_laevigata	RLG00000031964
rosa_multiflora	Rmu_co8223558.1_g000001 Rmu_sc0010496.1_g000006
rosa_roxburghii	Rroxscaffold_1G00063720
rosa_rugosa	Rorug05G0005000
rosa_samantha	Rh5AG098900 Rh5BG095700 Rh5CG107900 Rh5DG094400
rosa_wichuraiana	Rw5G008650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	821, 914
AccB1I	GGYRCC	1 cut(s)	215
AccB7I	CCANNNNNTGG	2 cut(s)	91, 597
AccI	GTMKAC	1 cut(s)	1133
AccII	CGCG	3 cut(s)	253, 488, 1229
AciI	CCGC	4 cut(s)	15, 70, 486, 1466
AclWI	GGATC	1 cut(s)	934
AcsI	RAATTY	4 cut(s)	730, 853, 931, 1021
AcuI	CTGAAG	1 cut(s)	299
AcyI	GRCGYC	1 cut(s)	674
AfaI	GTAC	3 cut(s)	434, 684, 788
AfiI	CCNNNNNNNGG	4 cut(s)	91, 212, 597, 705
AflIII	ACRYGT	1 cut(s)	1421
AgsI	TTSAA	7 cut(s)	196, 455, 955, 1094, 1372, 1400, 1496
AhlI	ACTAGT	1 cut(s)	1010
AjnI	CCWGG	1 cut(s)	577
AjuI	GAANNNNNNNTTGG	2 cut(s)	1013, 1045
AleI	CACNNNNGTG	1 cut(s)	1239
AluBI	AGCT	9 cut(s)	182, 283, 376, 534, 647, 722, 809, 908, 1076
AluI	AGCT	9 cut(s)	182, 283, 376, 534, 647, 722, 809, 908, 1076
Alw21I	GWGCWC	2 cut(s)	1018, 1416
Alw26I	GTCTC	2 cut(s)	319, 1204
AlwI	GGATC	1 cut(s)	934
AoxI	GGCC	1 cut(s)	1291
ApeKI	GCWGC	4 cut(s)	500, 809, 1571, 1610
ApoI	RAATTY	4 cut(s)	730, 853, 931, 1021
Asp700I	GAANNNNTTC	1 cut(s)	284
AspLEI	GCGC	3 cut(s)	253, 1229, 1231
AspS9I	GGNCC	1 cut(s)	1292
AsuC2I	CCSGG	2 cut(s)	213, 1713
AsuHPI	GGTGA	4 cut(s)	146, 583, 727, 1196
BanI	GGYRCC	1 cut(s)	215
BauI	CACGAG	1 cut(s)	316
Bbv12I	GWGCWC	2 cut(s)	1018, 1416
BbvI	GCAGC	4 cut(s)	487, 796, 1583, 1597
BccI	CCATC	5 cut(s)	661, 1151, 1223, 1501, 1647
BcgI	CGANNNNNNTGC	2 cut(s)	1220, 1254
BciT130I	CCWGG	1 cut(s)	579
BclI	TGATCA	1 cut(s)	330
BcnI	CCSGG	2 cut(s)	213, 1713
BcoDI	GTCTC	2 cut(s)	319, 1204
BcuI	ACTAGT	1 cut(s)	1010
BfaI	CTAG	2 cut(s)	173, 1011
BfmI	CTRYAG	1 cut(s)	1603
BisI	GCNGC	4 cut(s)	501, 810, 1572, 1611
BlsI	GCNGC	4 cut(s)	502, 811, 1573, 1612
Bme1390I	CCNGG	3 cut(s)	213, 579, 1713
BmgT120I	GGNCC	1 cut(s)	1292
BmiI	GGNNCC	2 cut(s)	217, 1294
BmrFI	CCNGG	3 cut(s)	213, 579, 1713
BmrI	ACTGGG	1 cut(s)	1323
BmsI	GCATC	6 cut(s)	262, 541, 564, 937, 1090, 1214
BmuI	ACTGGG	1 cut(s)	1323
Bpu10I	CCTNAGC	1 cut(s)	369
BpuMI	CCSGG	2 cut(s)	213, 1713
BsaBI	GATNNNNATC	1 cut(s)	925
BsaHI	GRCGYC	1 cut(s)	674
BsaI	GGTCTC	1 cut(s)	319
BsaJI	CCNNGG	1 cut(s)	578
BsaXI	ACNNNNNCTCC	4 cut(s)	654, 684, 1152, 1182
Bsc4I	CCNNNNNNNGG	4 cut(s)	91, 212, 597, 705
Bse1I	ACTGG	2 cut(s)	18, 1318
Bse8I	GATNNNNATC	1 cut(s)	925
BseBI	CCWGG	1 cut(s)	579
BseDI	CCNNGG	1 cut(s)	578
BseGI	GGATG	6 cut(s)	499, 530, 926, 1229, 1438, 1561
BseJI	GATNNNNATC	1 cut(s)	925
BseLI	CCNNNNNNNGG	4 cut(s)	91, 212, 597, 705
BseMII	CTCAG	5 cut(s)	19, 172, 314, 993, 1397
BseNI	ACTGG	2 cut(s)	18, 1318
BsePI	GCGCGC	1 cut(s)	1227
BseRI	GAGGAG	2 cut(s)	649, 1484
BseXI	GCAGC	4 cut(s)	487, 796, 1583, 1597
BseYI	CCCAGC	1 cut(s)	614
Bsh1236I	CGCG	3 cut(s)	253, 488, 1229
Bsh1285I	CGRYCG	1 cut(s)	1240
BshFI	GGCC	1 cut(s)	1293
BshNI	GGYRCC	1 cut(s)	215
BsiEI	CGRYCG	1 cut(s)	1240
BsiHKAI	GWGCWC	2 cut(s)	1018, 1416
BsiSI	CCGG	3 cut(s)	213, 392, 1712
BslFI	GGGAC	1 cut(s)	1455
BslI	CCNNNNNNNGG	4 cut(s)	91, 212, 597, 705
BsmAI	GTCTC	2 cut(s)	319, 1204
BsmFI	GGGAC	1 cut(s)	1455
BsmI	GAATGC	3 cut(s)	328, 592, 1543
BsnI	GGCC	1 cut(s)	1293
Bso31I	GGTCTC	1 cut(s)	319
Bsp1286I	GDGCHC	2 cut(s)	1018, 1416
Bsp143I	GATC	4 cut(s)	151, 330, 926, 975
BspACI	CCGC	4 cut(s)	15, 70, 486, 1466
BspANI	GGCC	1 cut(s)	1293
BspCNI	CTCAG	5 cut(s)	18, 171, 313, 992, 1396
BspFNI	CGCG	3 cut(s)	253, 488, 1229
BspLI	GGNNCC	2 cut(s)	217, 1294
BspPI	GGATC	1 cut(s)	934
BspT107I	GGYRCC	1 cut(s)	215
BspTNI	GGTCTC	1 cut(s)	319
BsrI	ACTGG	2 cut(s)	18, 1318
BssECI	CCNNGG	1 cut(s)	578
BssHII	GCGCGC	1 cut(s)	1227
BssMI	GATC	4 cut(s)	151, 330, 926, 975
BssNAI	GTATAC	1 cut(s)	1134
BssNI	GRCGYC	1 cut(s)	674
BssSI	CACGAG	1 cut(s)	316
Bst1107I	GTATAC	1 cut(s)	1134
Bst2BI	CACGAG	1 cut(s)	316
Bst2UI	CCWGG	1 cut(s)	579
Bst4CI	ACNGT	3 cut(s)	1238, 1604, 1702
Bst6I	CTCTTC	2 cut(s)	392, 535
BstACI	GRCGYC	1 cut(s)	674
BstC8I	GCNNGC	2 cut(s)	486, 1229
BstF5I	GGATG	6 cut(s)	499, 530, 926, 1229, 1438, 1561
BstFNI	CGCG	3 cut(s)	253, 488, 1229
BstHHI	GCGC	3 cut(s)	253, 1229, 1231
BstKTI	GATC	4 cut(s)	154, 333, 929, 978
BstMAI	GTCTC	2 cut(s)	319, 1204
BstMBI	GATC	4 cut(s)	151, 330, 926, 975
BstMCI	CGRYCG	1 cut(s)	1240
BstMWI	GCNNNNNNNGC	2 cut(s)	1568, 1665
BstNI	CCWGG	1 cut(s)	579
BstNSI	RCATGY	4 cut(s)	1070, 1332, 1425, 1489
BstSCI	CCNGG	3 cut(s)	211, 577, 1711
BstSFI	CTRYAG	1 cut(s)	1603
BstUI	CGCG	3 cut(s)	253, 488, 1229
BstV1I	GCAGC	4 cut(s)	487, 796, 1583, 1597
BstZ17I	GTATAC	1 cut(s)	1134
BsuRI	GGCC	1 cut(s)	1293
BtgZI	GCGATG	2 cut(s)	341, 1674
BtsCI	GGATG	6 cut(s)	499, 530, 926, 1229, 1438, 1561
BtsI	GCAGTG	1 cut(s)	140
BtsIMutI	CAGTG	1 cut(s)	140
Cac8I	GCNNGC	2 cut(s)	486, 1229
CfoI	GCGC	3 cut(s)	253, 1229, 1231
Cfr13I	GGNCC	1 cut(s)	1292
CseI	GACGC	1 cut(s)	663
Csp6I	GTAC	3 cut(s)	433, 683, 787
CviQI	GTAC	3 cut(s)	433, 683, 787
DpnI	GATC	4 cut(s)	153, 332, 928, 977
DpnII	GATC	4 cut(s)	151, 330, 926, 975
Eam1104I	CTCTTC	2 cut(s)	392, 535
EarI	CTCTTC	2 cut(s)	392, 535
Eco31I	GGTCTC	1 cut(s)	319
Eco57I	CTGAAG	1 cut(s)	299
EcoO109I	RGGNCCY	1 cut(s)	1292
EcoRI	GAATTC	4 cut(s)	730, 853, 931, 1021
EcoRII	CCWGG	1 cut(s)	577
EcoT22I	ATGCAT	1 cut(s)	952
FalI	AAGNNNNNCTT	4 cut(s)	171, 203, 1400, 1432
FaqI	GGGAC	1 cut(s)	1455
FauI	CCCGC	3 cut(s)	22, 77, 493
FbaI	TGATCA	1 cut(s)	330
FblI	GTMKAC	1 cut(s)	1133
Fnu4HI	GCNGC	4 cut(s)	501, 810, 1572, 1611
FokI	GGATG	6 cut(s)	506, 537, 933, 1236, 1445, 1568
Fsp4HI	GCNGC	4 cut(s)	501, 810, 1572, 1611
FspBI	CTAG	2 cut(s)	173, 1011
GlaI	GCGC	3 cut(s)	252, 1228, 1230
GluI	GCNGC	4 cut(s)	501, 810, 1572, 1611
GsaI	CCCAGC	1 cut(s)	618
HaeIII	GGCC	1 cut(s)	1293
HapII	CCGG	3 cut(s)	213, 392, 1712
HgaI	GACGC	1 cut(s)	663
HhaI	GCGC	3 cut(s)	253, 1229, 1231
Hin1I	GRCGYC	1 cut(s)	674
Hin6I	GCGC	3 cut(s)	251, 1227, 1229
HinP1I	GCGC	3 cut(s)	251, 1227, 1229
HindIII	AAGCTT	1 cut(s)	645
HinfI	GANTC	2 cut(s)	517, 984
HpaII	CCGG	3 cut(s)	213, 392, 1712
HphI	GGTGA	4 cut(s)	146, 583, 727, 1196
Hpy166II	GTNNAC	5 cut(s)	228, 414, 600, 1134, 1728
Hpy188III	TCNNGA	3 cut(s)	981, 1341, 1496
Hpy8I	GTNNAC	5 cut(s)	228, 414, 600, 1134, 1728
Hpy99I	CGWCG	1 cut(s)	258
HpyAV	CCTTC	3 cut(s)	197, 216, 949
HpyCH4III	ACNGT	3 cut(s)	1238, 1604, 1702
HpyCH4IV	ACGT	1 cut(s)	58
HpyCH4V	TGCA	9 cut(s)	439, 833, 840, 883, 950, 1328, 1351, 1543, 1646
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1568, 1665
HpySE526I	ACGT	1 cut(s)	58
Hsp92I	GRCGYC	1 cut(s)	674
HspAI	GCGC	3 cut(s)	251, 1227, 1229
Ksp22I	TGATCA	1 cut(s)	330
Kzo9I	GATC	4 cut(s)	151, 330, 926, 975
LmnI	GCTCC	4 cut(s)	373, 708, 1411, 1656
Lsp1109I	GCAGC	4 cut(s)	487, 796, 1583, 1597
LweI	GCATC	6 cut(s)	262, 541, 564, 937, 1090, 1214
MaeI	CTAG	2 cut(s)	173, 1011
MaeII	ACGT	1 cut(s)	58
MaeIII	GTNAC	3 cut(s)	138, 715, 1275
MalI	GATC	4 cut(s)	153, 332, 928, 977
MboI	GATC	4 cut(s)	151, 330, 926, 975
MboII	GAAGA	7 cut(s)	409, 552, 595, 993, 1201, 1472, 1693
MhlI	GDGCHC	2 cut(s)	1018, 1416
MlyI	GAGTC	1 cut(s)	993
Mph1103I	ATGCAT	1 cut(s)	952
MroXI	GAANNNNTTC	1 cut(s)	284
MseI	TTAA	4 cut(s)	870, 1080, 1173, 1266
MslI	CAYNNNNRTG	3 cut(s)	888, 945, 1239
MspA1I	CMGCKG	1 cut(s)	1076
MspI	CCGG	3 cut(s)	213, 392, 1712
MspR9I	CCNGG	3 cut(s)	213, 579, 1713
Mva1269I	GAATGC	3 cut(s)	328, 592, 1543
MvaI	CCWGG	1 cut(s)	579
MvnI	CGCG	3 cut(s)	253, 488, 1229
MwoI	GCNNNNNNNGC	2 cut(s)	1568, 1665
NciI	CCSGG	2 cut(s)	213, 1713
NdeII	GATC	4 cut(s)	151, 330, 926, 975
NlaIV	GGNNCC	2 cut(s)	217, 1294
NmuCI	GTSAC	2 cut(s)	138, 715
NsiI	ATGCAT	1 cut(s)	952
NspI	RCATGY	4 cut(s)	1070, 1332, 1425, 1489
OliI	CACNNNNGTG	1 cut(s)	1239
PauI	GCGCGC	1 cut(s)	1227
PciI	ACATGT	1 cut(s)	1421
PcsI	WCGNNNNNNNCGW	1 cut(s)	323
PctI	GAATGC	3 cut(s)	328, 592, 1543
PdmI	GAANNNNTTC	1 cut(s)	284
PfeI	GAWTC	1 cut(s)	517
PflMI	CCANNNNNTGG	2 cut(s)	91, 597
PkrI	GCNGC	4 cut(s)	502, 811, 1573, 1612
PleI	GAGTC	1 cut(s)	992
PpsI	GAGTC	1 cut(s)	992
PscI	ACATGT	1 cut(s)	1421
PsiI	TTATAA	2 cut(s)	821, 914
Psp6I	CCWGG	1 cut(s)	577
PspFI	CCCAGC	1 cut(s)	614
PspGI	CCWGG	1 cut(s)	577
PspN4I	GGNNCC	2 cut(s)	217, 1294
PspPI	GGNCC	1 cut(s)	1292
PteI	GCGCGC	1 cut(s)	1227
PvuII	CAGCTG	1 cut(s)	1076
RsaI	GTAC	3 cut(s)	434, 684, 788
RsaNI	GTAC	3 cut(s)	433, 683, 787
RseI	CAYNNNNRTG	3 cut(s)	888, 945, 1239
SaqAI	TTAA	4 cut(s)	870, 1080, 1173, 1266
SatI	GCNGC	4 cut(s)	501, 810, 1572, 1611
Sau3AI	GATC	4 cut(s)	151, 330, 926, 975
Sau96I	GGNCC	1 cut(s)	1292
SchI	GAGTC	1 cut(s)	993
ScrFI	CCNGG	3 cut(s)	213, 579, 1713
SduI	GDGCHC	2 cut(s)	1018, 1416
SfaNI	GCATC	6 cut(s)	262, 541, 564, 937, 1090, 1214
SfcI	CTRYAG	1 cut(s)	1603
SmiMI	CAYNNNNRTG	3 cut(s)	888, 945, 1239
SpeI	ACTAGT	1 cut(s)	1010
SsiI	CCGC	4 cut(s)	15, 70, 486, 1466
SspMI	CTAG	2 cut(s)	173, 1011
StyD4I	CCNGG	3 cut(s)	211, 577, 1711
TaaI	ACNGT	3 cut(s)	1238, 1604, 1702
TaiI	ACGT	1 cut(s)	61
TaqI	TCGA	4 cut(s)	288, 929, 1628, 1718
TfiI	GAWTC	1 cut(s)	517
Tru1I	TTAA	4 cut(s)	870, 1080, 1173, 1266
Tru9I	TTAA	4 cut(s)	870, 1080, 1173, 1266
TscAI	CASTG	1 cut(s)	147
TseFI	GTSAC	2 cut(s)	138, 715
TseI	GCWGC	4 cut(s)	500, 809, 1571, 1610
Tsp45I	GTSAC	2 cut(s)	138, 715
TspRI	CASTG	1 cut(s)	147
Van91I	CCANNNNNTGG	2 cut(s)	91, 597
XapI	RAATTY	4 cut(s)	730, 853, 931, 1021
XceI	RCATGY	4 cut(s)	1070, 1332, 1425, 1489
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1555
XmiI	GTMKAC	1 cut(s)	1133
XmnI	GAANNNNTTC	1 cut(s)	284
XspI	CTAG	2 cut(s)	173, 1011
Zsp2I	ATGCAT	1 cut(s)	952

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.