FvH4_5g30590

clathrin assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014227
Physical Location & Seq
Forward (+)
21476523 .. 21478974
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30590.t1

Sequence Viewer

Length: 1689 bp
ATGGCTTCAAGTAAATTCAGAAGAGCCTTAGGTGCCGTGAAGGACCAAACTAGCATTGGCCTTGCCAAAGTTGGCAGCAGCAATTCTCTAGCGGACCTCGACGTTGCCATTGTTAAGGCTACGAGGCATGAAGAATACCCAACCGAAGAGAAATACATAAGAGAGATTCTTAGCTTAACATGCTACTCACGCGCATACATCAGTGCCTGTGTCAACACCATCTCCAGGCGCCTCAACAAAACCAAGAACTGGATTGTTGCCCTCAAAACCCTCATGTTGATCCAACGCCTGCTTTCCGAGGGTGATCCTGCATATGAACAAGAGATATTCTTCTCCACAAGACGTGGCACTAGGTTTCTTAACATGTCTGATTTTAGGGACACTTCTGGCTCCAATACATGGGACTACTCTGCCTTTGTGCGCACGTATGCATTGTACCTCGACGAACGGCTCGAGTTTCGAATGCAAAGCCGACGTGGGAAACGCAGTGTGTTCGGAGTAATAGATGACGAGGAGGCAGAACCGGTACCGGAGCAAAATGGTAACCAGCAAGTGTGTAATAGGGCTACACCTGTGCATGAAATGAGGCACGATAAGTTGTTTTCAAGGATGCAACATTTGCAGCAGCTCCTTGAACGGTTTCTAGCGACACGTCCAACAGGTGAAGCGAAGAACAACAGGGTTATAATAGTTGCCCTATACCTGCTAGTGAAAGAGAGCTTCCAAATATATTATGACGTAACAGAGATAATGGGGATCTTCATCGACCGCTTCATGGAGCTTGAGCTCCGAGTACCCCCCGATGTCGAGAGAATCACACCCAAGAAGCTCGAGGTGATGGACGAGTACATTCGAGACAAGTCGGCTATGGAGCAAGGCCGGAAAGTAATTAGGGCACTAGAGCCGAGAGGTGACTCCCCTGTGTCTGTGGAGTCCAAAGAAGAACCAGACATGAACTCCATTAAAGCACTACCAGCGCCGGAAGACGTCGCGGAGGCTCCGGTGGAGGAAGAGAAGGAAGAAGAACCGGTTGTGGAGAAAGAAGAACCGAAGAAACAAGACGTGGGTGATTTGTTGAACTTGGGAGAAGACGCCGGCTCTAGTGAACAACAAGGTGACAAACTAGCGTTGGCATTGTTCAATGGGGAGGAGGGTGCTACGACCTCCACAACGGGTCCGCAACCGTGGGAGGCGTTTTCTGAGGAAGCCGATTGGGAAAAGGCTCTTGTCCAATCTGCAACTCACTTGCCAAATCAGAAGACCTCTCTCGGAGGAGGGTTCGACTTGTTGCTACTGGACGGAATGTACCAACAAGCTCAGGTAACGTCGGCGATGGATGGCCCTGGTTATGGAACCAGCGGCAATACAAGTAGCGTTGCATTAGGCTCTACAGGAAGGCCGGCCATGCTAGCATTGCCTGCGCCACCAGCACAGTCAGATCAGAATGGGGAGAGTTCTATGGTTGTTGCAACAACGCAGGTAGATCCGTTTGCCGCCTCGATGGTGGTGCCGCCGCCGGCGTACGTGCAAATGTCGGATTTGGAGAAGAAGCAGAAGCTGTTGATGGAAGAACAGTTGATGTGGCACCAGTACGCAAGGGATGGGATGCAAGGGCAGATTGGATTGACGAAGCTGCAACATAATCCTCATTATCATCCAAACGTGGGAGGTTACACACACACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

563

Amino Acids

62.96

Weight (kDa)

5.1

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 32 - 261 5.5e-68 ANTH domain
ENTH PF01417 34 - 144 1.6e-06 ENTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014227)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03050
fragaria_vesca FvH4_5g30570 FvH4_5g30590
malus_domestica MD08G1222400.v1.1 MD15G1415800.v1.1
prunus_persica Prupe.1G560200_v2.0.a1
pyrus_communis pycom15g36900
rosa_chinensis RchiOBHm_Chr7g0225461
rosa_laevigata RLG00000001817
rosa_multiflora Rmu_sc0000259.1_g000009
rosa_roxburghii Rroxscaffold_3G00233800
rosa_rugosa Rorug07G0229600.1
rosa_samantha Rh7AG373600 Rh7BG364300 Rh7CG391700 Rh7DG375500
rosa_wichuraiana Rw7G031680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 686
AatII GACGTC 1 cut(s) 990
Acc16I TGCGCA 1 cut(s) 422
Acc36I ACCTGC 2 cut(s) 711, 1468
Acc65I GGTACC 1 cut(s) 526
AccB1I GGYRCC 5 cut(s) 32, 228, 526, 1507, 1584
AccB7I CCANNNNNTGG 2 cut(s) 249, 399
AccII CGCG 2 cut(s) 192, 992
AciI CCGC 8 cut(s) 92, 769, 992, 1178, 1359, 1494, 1511, 1514
AclWI GGATC 4 cut(s) 274, 299, 764, 1478
AcoI YGGCCR 1 cut(s) 1401
AcsI RAATTY 1 cut(s) 14
AcyI GRCGYC 3 cut(s) 229, 987, 1092
AfaI GTAC 7 cut(s) 437, 528, 795, 848, 1307, 1523, 1592
AfiI CCNNNNNNNGG 6 cut(s) 225, 249, 399, 775, 1349, 1664
AflIII ACRYGT 2 cut(s) 363, 650
AgeI ACCGGT 2 cut(s) 523, 1027
AgsI TTSAA 5 cut(s) 9, 606, 635, 1078, 1141
AjiI CACGTC 4 cut(s) 344, 476, 653, 1063
AjnI CCWGG 2 cut(s) 224, 1342
AluBI AGCT 9 cut(s) 174, 628, 720, 781, 787, 829, 1316, 1558, 1633
AluI AGCT 9 cut(s) 174, 628, 720, 781, 787, 829, 1316, 1558, 1633
Alw21I GWGCWC 1 cut(s) 789
Alw26I GTCTC 1 cut(s) 849
AlwI GGATC 4 cut(s) 274, 299, 764, 1478
AlwNI CAGNNNCTG 2 cut(s) 207, 1558
Ama87I CYCGRG 2 cut(s) 452, 830
AoxI GGCC 5 cut(s) 58, 877, 1339, 1397, 1401
ApeKI GCWGC 5 cut(s) 75, 78, 622, 625, 1633
ApoI RAATTY 1 cut(s) 14
AsiGI ACCGGT 2 cut(s) 523, 1027
Asp700I GAANNNNTTC 1 cut(s) 639
Asp718I GGTACC 1 cut(s) 526
AspLEI GCGC 5 cut(s) 194, 231, 423, 979, 1423
AspS9I GGNCC 4 cut(s) 43, 94, 1175, 1340
AsuHPI GGTGA 6 cut(s) 314, 674, 847, 923, 1079, 1127
AsuII TTCGAA 1 cut(s) 460
AsuNHI GCTAGC 1 cut(s) 1408
AvaI CYCGRG 2 cut(s) 452, 830
AvaII GGWCC 3 cut(s) 43, 94, 1175
AxyI CCTNAGG 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 898
BanI GGYRCC 5 cut(s) 32, 228, 526, 1507, 1584
BanII GRGCYC 1 cut(s) 789
BbsI GAAGAC 3 cut(s) 990, 1095, 1265
Bbv12I GWGCWC 1 cut(s) 789
BbvI GCAGC 5 cut(s) 87, 90, 634, 637, 1620
BccI CCATC 7 cut(s) 227, 832, 1327, 1331, 1495, 1558, 1595
BceAI ACGGC 2 cut(s) 20, 464
BcgI CGANNNNNNTGC 4 cut(s) 475, 509, 1489, 1523
BciT130I CCWGG 2 cut(s) 226, 1344
BcoDI GTCTC 1 cut(s) 849
BfaI CTAG 9 cut(s) 51, 89, 351, 644, 707, 899, 1101, 1124, 1409
BfmI CTRYAG 1 cut(s) 1389
BfoI RGCGCY 2 cut(s) 232, 980
BfuAI ACCTGC 2 cut(s) 711, 1468
BisI GCNGC 9 cut(s) 76, 79, 623, 626, 1360, 1494, 1511, 1514, 1634
BlsI GCNGC 9 cut(s) 77, 80, 624, 627, 1361, 1495, 1512, 1515, 1635
Bme1390I CCNGG 2 cut(s) 226, 1344
Bme18I GGWCC 3 cut(s) 43, 94, 1175
BmeT110I CYCGRG 2 cut(s) 452, 830
BmgBI CACGTC 4 cut(s) 344, 476, 653, 1063
BmgT120I GGNCC 4 cut(s) 43, 94, 1175, 1340
BmiI GGNNCC 9 cut(s) 34, 230, 391, 528, 999, 1176, 1354, 1509, 1586
BmrFI CCNGG 2 cut(s) 226, 1344
BmsI GCATC 2 cut(s) 600, 1596
BmtI GCTAGC 1 cut(s) 1412
BpiI GAAGAC 3 cut(s) 990, 1095, 1265
BpmI CTGGAG 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 1317
Bpu14I TTCGAA 1 cut(s) 460
BpuEI CTTGAG 1 cut(s) 803
BsaAI YACGTR 2 cut(s) 426, 1525
BsaBI GATNNNNATC 1 cut(s) 761
BsaHI GRCGYC 3 cut(s) 229, 987, 1092
BsaJI CCNNGG 3 cut(s) 297, 1184, 1342
BsaWI WCCGGW 4 cut(s) 523, 529, 1000, 1027
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 941, 971
Bsc4I CCNNNNNNNGG 6 cut(s) 225, 249, 399, 775, 1349, 1664
Bse118I RCCGGY 5 cut(s) 523, 1027, 1094, 1399, 1516
Bse1I ACTGG 3 cut(s) 254, 1299, 1588
Bse21I CCTNAGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 1412
Bse8I GATNNNNATC 1 cut(s) 761
BseBI CCWGG 2 cut(s) 226, 1344
BseDI CCNNGG 3 cut(s) 297, 1184, 1342
BseGI GGATG 5 cut(s) 615, 1342, 1606, 1611, 1654
BseJI GATNNNNATC 1 cut(s) 761
BseLI CCNNNNNNNGG 6 cut(s) 225, 249, 399, 775, 1349, 1664
BseMI GCAATG 1 cut(s) 1412
BseMII CTCAG 2 cut(s) 1191, 1331
BseNI ACTGG 3 cut(s) 254, 1299, 1588
BseRI GAGGAG 3 cut(s) 527, 1163, 1287
BseSI GKGCMC 1 cut(s) 898
BseXI GCAGC 5 cut(s) 87, 90, 634, 637, 1620
Bsh1236I CGCG 2 cut(s) 192, 992
Bsh1285I CGRYCG 1 cut(s) 769
BshFI GGCC 5 cut(s) 60, 879, 1341, 1399, 1403
BshNI GGYRCC 5 cut(s) 32, 228, 526, 1507, 1584
BshTI ACCGGT 2 cut(s) 523, 1027
BsiEI CGRYCG 1 cut(s) 769
BsiHKAI GWGCWC 1 cut(s) 789
BsiHKCI CYCGRG 2 cut(s) 452, 830
BsiSI CCGG 9 cut(s) 524, 530, 880, 980, 1001, 1028, 1095, 1400, 1517
BsiWI CGTACG 1 cut(s) 1521
BslFI GGGAC 2 cut(s) 392, 416
BslI CCNNNNNNNGG 6 cut(s) 225, 249, 399, 775, 1349, 1664
BsmAI GTCTC 1 cut(s) 849
BsmFI GGGAC 2 cut(s) 392, 416
BsmI GAATGC 1 cut(s) 468
BsnI GGCC 5 cut(s) 60, 879, 1341, 1399, 1403
BsoBI CYCGRG 2 cut(s) 452, 830
Bsp119I TTCGAA 1 cut(s) 460
Bsp1286I GDGCHC 2 cut(s) 789, 898
Bsp143I GATC 5 cut(s) 279, 304, 756, 1438, 1483
BspACI CCGC 8 cut(s) 92, 769, 992, 1178, 1359, 1494, 1511, 1514
BspANI GGCC 5 cut(s) 60, 879, 1341, 1399, 1403
BspCNI CTCAG 2 cut(s) 1192, 1330
BspFNI CGCG 2 cut(s) 192, 992
BspLI GGNNCC 9 cut(s) 34, 230, 391, 528, 999, 1176, 1354, 1509, 1586
BspMI ACCTGC 2 cut(s) 711, 1468
BspOI GCTAGC 1 cut(s) 1412
BspPI GGATC 4 cut(s) 274, 299, 764, 1478
BspQI GCTCTTC 1 cut(s) 16
BspT104I TTCGAA 1 cut(s) 460
BspT107I GGYRCC 5 cut(s) 32, 228, 526, 1507, 1584
BsrDI GCAATG 1 cut(s) 1412
BsrFI RCCGGY 5 cut(s) 523, 1027, 1094, 1399, 1516
BsrI ACTGG 3 cut(s) 254, 1299, 1588
BssAI RCCGGY 5 cut(s) 523, 1027, 1094, 1399, 1516
BssECI CCNNGG 3 cut(s) 297, 1184, 1342
BssMI GATC 5 cut(s) 279, 304, 756, 1438, 1483
BssNI GRCGYC 3 cut(s) 229, 987, 1092
Bst2UI CCWGG 2 cut(s) 226, 1344
Bst4CI ACNGT 4 cut(s) 639, 1185, 1434, 1575
Bst6I CTCTTC 3 cut(s) 16, 141, 1005
BstACI GRCGYC 3 cut(s) 229, 987, 1092
BstAPI GCANNNNNTGC 2 cut(s) 619, 1418
BstBAI YACGTR 2 cut(s) 426, 1525
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 6 cut(s) 290, 1096, 1401, 1410, 1419, 1518
BstDEI CTNAG 4 cut(s) 28, 170, 1200, 1317
BstDSI CCRYGG 1 cut(s) 1184
BstEII GGTNACC 1 cut(s) 542
BstF5I GGATG 5 cut(s) 615, 1342, 1606, 1611, 1654
BstFNI CGCG 2 cut(s) 192, 992
BstH2I RGCGCY 2 cut(s) 232, 980
BstHHI GCGC 5 cut(s) 194, 231, 423, 979, 1423
BstKTI GATC 5 cut(s) 282, 307, 759, 1441, 1486
BstMAI GTCTC 1 cut(s) 849
BstMBI GATC 5 cut(s) 279, 304, 756, 1438, 1483
BstMCI CGRYCG 1 cut(s) 769
BstNI CCWGG 2 cut(s) 226, 1344
BstNSI RCATGY 2 cut(s) 183, 367
BstPI GGTNACC 1 cut(s) 542
BstSCI CCNGG 2 cut(s) 224, 1342
BstSFI CTRYAG 1 cut(s) 1389
BstSLI GKGCMC 1 cut(s) 898
BstUI CGCG 2 cut(s) 192, 992
BstV1I GCAGC 5 cut(s) 87, 90, 634, 637, 1620
BstV2I GAAGAC 3 cut(s) 990, 1095, 1265
BstX2I RGATCY 2 cut(s) 756, 1483
BstYI RGATCY 2 cut(s) 756, 1483
Bsu36I CCTNAGG 1 cut(s) 28
BsuRI GGCC 5 cut(s) 60, 879, 1341, 1399, 1403
BtgI CCRYGG 1 cut(s) 1184
BtgZI GCGATG 1 cut(s) 1346
BtrI CACGTC 4 cut(s) 344, 476, 653, 1063
BtsCI GGATG 5 cut(s) 615, 1342, 1606, 1611, 1654
BtsI GCAGTG 1 cut(s) 493
BtsIMutI CAGTG 2 cut(s) 208, 493
BveI ACCTGC 2 cut(s) 711, 1468
Cac8I GCNNGC 6 cut(s) 290, 1096, 1401, 1410, 1419, 1518
CaiI CAGNNNCTG 2 cut(s) 207, 1558
CfoI GCGC 5 cut(s) 194, 231, 423, 979, 1423
Cfr10I RCCGGY 5 cut(s) 523, 1027, 1094, 1399, 1516
Cfr13I GGNCC 4 cut(s) 43, 94, 1175, 1340
CseI GACGC 1 cut(s) 1100
Csp6I GTAC 7 cut(s) 436, 527, 794, 847, 1306, 1522, 1591
CspAI ACCGGT 2 cut(s) 523, 1027
CviAII CATG 9 cut(s) 128, 180, 274, 364, 399, 578, 775, 952, 1405
CviQI GTAC 7 cut(s) 436, 527, 794, 847, 1306, 1522, 1591
DdeI CTNAG 4 cut(s) 28, 170, 1200, 1317
DinI GGCGCC 1 cut(s) 230
DpnI GATC 5 cut(s) 281, 306, 758, 1440, 1485
DpnII GATC 5 cut(s) 279, 304, 756, 1438, 1483
EaeI YGGCCR 1 cut(s) 1401
Eam1104I CTCTTC 3 cut(s) 16, 141, 1005
EarI CTCTTC 3 cut(s) 16, 141, 1005
Ecl136II GAGCTC 1 cut(s) 787
Eco24I GRGCYC 1 cut(s) 789
Eco47I GGWCC 3 cut(s) 43, 94, 1175
Eco53kI GAGCTC 1 cut(s) 787
Eco81I CCTNAGG 1 cut(s) 28
Eco88I CYCGRG 2 cut(s) 452, 830
Eco91I GGTNACC 1 cut(s) 542
EcoICRI GAGCTC 1 cut(s) 787
EcoO65I GGTNACC 1 cut(s) 542
EcoRII CCWGG 2 cut(s) 224, 1342
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 789
EgeI GGCGCC 1 cut(s) 230
EheI GGCGCC 1 cut(s) 230
FaeI CATG 9 cut(s) 131, 183, 277, 367, 402, 581, 778, 955, 1408
FaqI GGGAC 2 cut(s) 392, 416
FatI CATG 9 cut(s) 127, 179, 273, 363, 398, 577, 774, 951, 1404
FauNDI CATATG 1 cut(s) 313
Fnu4HI GCNGC 9 cut(s) 76, 79, 623, 626, 1360, 1494, 1511, 1514, 1634
FokI GGATG 5 cut(s) 622, 1349, 1613, 1618, 1641
FriOI GRGCYC 1 cut(s) 789
FseI GGCCGGCC 1 cut(s) 1403
Fsp4HI GCNGC 9 cut(s) 76, 79, 623, 626, 1360, 1494, 1511, 1514, 1634
FspAI RTGCGCAY 1 cut(s) 422
FspBI CTAG 9 cut(s) 51, 89, 351, 644, 707, 899, 1101, 1124, 1409
FspI TGCGCA 1 cut(s) 422
GlaI GCGC 5 cut(s) 193, 230, 422, 978, 1422
GluI GCNGC 9 cut(s) 76, 79, 623, 626, 1360, 1494, 1511, 1514, 1634
GsuI CTGGAG 1 cut(s) 208
HaeII RGCGCY 2 cut(s) 232, 980
HaeIII GGCC 5 cut(s) 60, 879, 1341, 1399, 1403
HapII CCGG 9 cut(s) 524, 530, 880, 980, 1001, 1028, 1095, 1400, 1517
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 5 cut(s) 194, 231, 423, 979, 1423
Hin1I GRCGYC 3 cut(s) 229, 987, 1092
Hin1II CATG 9 cut(s) 131, 183, 277, 367, 402, 581, 778, 955, 1408
Hin6I GCGC 5 cut(s) 192, 229, 421, 977, 1421
HinP1I GCGC 5 cut(s) 192, 229, 421, 977, 1421
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 4 cut(s) 166, 813, 914, 932
HpaII CCGG 9 cut(s) 524, 530, 880, 980, 1001, 1028, 1095, 1400, 1517
HphI GGTGA 6 cut(s) 314, 674, 847, 923, 1079, 1127
Hpy166II GTNNAC 2 cut(s) 214, 1106
Hpy188III TCNNGA 2 cut(s) 808, 854
Hpy8I GTNNAC 2 cut(s) 214, 1106
Hpy99I CGWCG 5 cut(s) 104, 446, 477, 992, 1330
HpyAV CCTTC 3 cut(s) 34, 1009, 1389
HpyCH4III ACNGT 4 cut(s) 639, 1185, 1434, 1575
HpyF3I CTNAG 4 cut(s) 28, 170, 1200, 1317
Hsp92I GRCGYC 3 cut(s) 229, 987, 1092
Hsp92II CATG 9 cut(s) 131, 183, 277, 367, 402, 581, 778, 955, 1408
HspAI GCGC 5 cut(s) 192, 229, 421, 977, 1421
KasI GGCGCC 1 cut(s) 228
KpnI GGTACC 1 cut(s) 530
KroI GCCGGC 3 cut(s) 1094, 1399, 1516
KroNI GCCGGC 3 cut(s) 1096, 1401, 1518
Kzo9I GATC 5 cut(s) 279, 304, 756, 1438, 1483
LguI GCTCTTC 1 cut(s) 16
LmnI GCTCC 7 cut(s) 395, 532, 633, 778, 792, 871, 1003
Lsp1109I GCAGC 5 cut(s) 87, 90, 634, 637, 1620
LweI GCATC 2 cut(s) 600, 1596
MaeI CTAG 9 cut(s) 51, 89, 351, 644, 707, 899, 1101, 1124, 1409
MaeIII GTNAC 6 cut(s) 542, 739, 911, 1115, 1321, 1670
MalI GATC 5 cut(s) 281, 306, 758, 1440, 1485
MboI GATC 5 cut(s) 279, 304, 756, 1438, 1483
MflI RGATCY 2 cut(s) 756, 1483
MhlI GDGCHC 2 cut(s) 789, 898
MluCI AATT 3 cut(s) 14, 82, 888
Mly113I GGCGCC 1 cut(s) 229
MlyI GAGTC 2 cut(s) 908, 941
MmeI TCCRAC 3 cut(s) 307, 680, 1515
Mph1103I ATGCAT 1 cut(s) 433
MreI CGCCGGCG 1 cut(s) 1516
MroNI GCCGGC 3 cut(s) 1094, 1399, 1516
MroXI GAANNNNTTC 1 cut(s) 639
MseI TTAA 4 cut(s) 114, 176, 360, 963
MspA1I CMGCKG 1 cut(s) 1359
MspI CCGG 9 cut(s) 524, 530, 880, 980, 1001, 1028, 1095, 1400, 1517
MspR9I CCNGG 2 cut(s) 226, 1344
Mva1269I GAATGC 1 cut(s) 468
MvaI CCWGG 2 cut(s) 226, 1344
MvnI CGCG 2 cut(s) 192, 992
NaeI GCCGGC 3 cut(s) 1096, 1401, 1518
NarI GGCGCC 1 cut(s) 229
NdeI CATATG 1 cut(s) 313
NdeII GATC 5 cut(s) 279, 304, 756, 1438, 1483
NgoMIV GCCGGC 3 cut(s) 1094, 1399, 1516
NheI GCTAGC 1 cut(s) 1408
NlaIII CATG 9 cut(s) 131, 183, 277, 367, 402, 581, 778, 955, 1408
NlaIV GGNNCC 9 cut(s) 34, 230, 391, 528, 999, 1176, 1354, 1509, 1586
NmeAIII GCCGAG 1 cut(s) 930
NmuCI GTSAC 2 cut(s) 911, 1115
NsbI TGCGCA 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 2 cut(s) 183, 367
NspV TTCGAA 1 cut(s) 460
PaeR7I CTCGAG 2 cut(s) 452, 830
PciI ACATGT 1 cut(s) 363
PciSI GCTCTTC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PctI GAATGC 1 cut(s) 468
PdiI GCCGGC 3 cut(s) 1096, 1401, 1518
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 2 cut(s) 166, 813
Pfl23II CGTACG 1 cut(s) 1521
PflMI CCANNNNNTGG 2 cut(s) 249, 399
PinAI ACCGGT 2 cut(s) 523, 1027
PkrI GCNGC 9 cut(s) 77, 80, 624, 627, 1361, 1495, 1512, 1515, 1635
PleI GAGTC 2 cut(s) 908, 940
PluTI GGCGCC 1 cut(s) 232
PpsI GAGTC 2 cut(s) 908, 940
Ppu21I YACGTR 2 cut(s) 426, 1525
PscI ACATGT 1 cut(s) 363
PsiI TTATAA 1 cut(s) 686
Psp124BI GAGCTC 1 cut(s) 789
Psp6I CCWGG 2 cut(s) 224, 1342
PspEI GGTNACC 1 cut(s) 542
PspGI CCWGG 2 cut(s) 224, 1342
PspLI CGTACG 1 cut(s) 1521
PspN4I GGNNCC 9 cut(s) 34, 230, 391, 528, 999, 1176, 1354, 1509, 1586
PspPI GGNCC 4 cut(s) 43, 94, 1175, 1340
PspXI VCTCGAGB 2 cut(s) 452, 830
PstNI CAGNNNCTG 2 cut(s) 207, 1558
PsuI RGATCY 2 cut(s) 756, 1483
RigI GGCCGGCC 1 cut(s) 1403
RsaI GTAC 7 cut(s) 437, 528, 795, 848, 1307, 1523, 1592
RsaNI GTAC 7 cut(s) 436, 527, 794, 847, 1306, 1522, 1591
SacI GAGCTC 1 cut(s) 789
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 4 cut(s) 114, 176, 360, 963
SatI GCNGC 9 cut(s) 76, 79, 623, 626, 1360, 1494, 1511, 1514, 1634
Sau3AI GATC 5 cut(s) 279, 304, 756, 1438, 1483
Sau96I GGNCC 4 cut(s) 43, 94, 1175, 1340
SchI GAGTC 2 cut(s) 908, 941
ScrFI CCNGG 2 cut(s) 226, 1344
SduI GDGCHC 2 cut(s) 789, 898
SfaNI GCATC 2 cut(s) 600, 1596
SfcI CTRYAG 1 cut(s) 1389
SfoI GGCGCC 1 cut(s) 230
Sfr274I CTCGAG 2 cut(s) 452, 830
SfuI TTCGAA 1 cut(s) 460
SgrAI CRCCGGYG 1 cut(s) 1516
SinI GGWCC 3 cut(s) 43, 94, 1175
SlaI CTCGAG 2 cut(s) 452, 830
SmlI CTYRAG 3 cut(s) 452, 782, 830
SmoI CTYRAG 3 cut(s) 452, 782, 830
Sse9I AATT 3 cut(s) 14, 82, 888
SsiI CCGC 8 cut(s) 92, 769, 992, 1178, 1359, 1494, 1511, 1514
SspDI GGCGCC 1 cut(s) 228
SspMI CTAG 9 cut(s) 51, 89, 351, 644, 707, 899, 1101, 1124, 1409
SstI GAGCTC 1 cut(s) 789
StyD4I CCNGG 2 cut(s) 224, 1342
TaaI ACNGT 4 cut(s) 639, 1185, 1434, 1575
TasI AATT 3 cut(s) 14, 82, 888
TatI WGTACW 1 cut(s) 846
TauI GCSGC 4 cut(s) 1362, 1496, 1513, 1516
TfiI GAWTC 2 cut(s) 166, 813
Tru1I TTAA 4 cut(s) 114, 176, 360, 963
Tru9I TTAA 4 cut(s) 114, 176, 360, 963
TscAI CASTG 2 cut(s) 208, 493
TseFI GTSAC 2 cut(s) 911, 1115
TseI GCWGC 5 cut(s) 75, 78, 622, 625, 1633
Tsp45I GTSAC 2 cut(s) 911, 1115
TspDTI ATGAA 6 cut(s) 144, 330, 594, 751, 763, 968
TspGWI ACGGA 2 cut(s) 1314, 1476
TspRI CASTG 2 cut(s) 208, 493
Van91I CCANNNNNTGG 2 cut(s) 249, 399
VpaK11BI GGWCC 3 cut(s) 43, 94, 1175
XapI RAATTY 1 cut(s) 14
XceI RCATGY 2 cut(s) 183, 367
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XhoI CTCGAG 2 cut(s) 452, 830
XmnI GAANNNNTTC 1 cut(s) 639
XspI CTAG 9 cut(s) 51, 89, 351, 644, 707, 899, 1101, 1124, 1409
ZraI GACGTC 1 cut(s) 988
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.