FvH4_5g33502

Protein misato homolog 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005482
Physical Location & Seq
Forward (+)
24223014 .. 24225840
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33502.t1

Sequence Viewer

Length: 1356 bp
ATGAGTTCATCTGGAACTGTGTATAATGGGGGTTCATCTGTACCATCAAGAGTTATGACATGGGATGATAATGTATCTACTCAAGCCTCTGAACTGCATCGGAGAAATTTGTTCTTGCAAAGTTTGTACCAGGAAGAGCAGGAGAATTCTTTTACTGTTGAAAATGGTGTCAGTGGTGTGCAGAATGGTTCTCAAAAAGAAGTTTCTGACAGAGACATTGTGGAAGAGGATTCCTTTTCTGGGGGTTTACATGGAGAAGAAATGAGTGACAGACTTCGCTTTTTCATAGAAGAGTGTGACCATATTCAGGGTTTCCAGTTCATTGTTGATGACTCAGGAGGTTTCTCTCCGATAGCAGTTGATTTTCTGGAGAGTATTGCAGATGAATACACAAATGTTCCTGTGTTGCTCTATGCTGTATGGGGTCCTTGCTCTGCGATAGATTCCATGAGCCAAAAGCATAGAGTCAGCAAGAAGCTTCATGATGCGGTTTCATTTTCAAGGCTATCTTCTTTATGTAAACTGATTGTTCCAGTCGGGCTACCTTCACTGAGTAAAAGCAAAGCATCAAGGCTCCTCTGCATTAAAGATGAAAAGCCTTATCACTGCAGTGCAATGTATGCTGCTACCCTACATTCTGTCAGCATGCCTTTCCGAATGGAACCACTTGGGCCCAGTGCAGACTCAACTTATGCTTCTGGTGCTTTGACTGTTAATGAGGTTGTGCAAATCCTATCAGGGCAAGCTAGACAGAATATGGTCGCTATTCTGGATGCTGCAATGCCAGCACCTTCTTTAGCTTATATTATTTCGGTTGCTTGTCATCCACTGACACCAGAGGTTTCAGAGCATGTTGAAGACTTACAGTCAGTAGAATCCATGATTGTCCTCGGCGCCCTTGGAACAGGAGGCCAGCAAGCATCAATTAATGAAGTAAAAAGTATGGTACACGTGGCTTATGAGCATGCAATAACAAGGCCAATCTTTAGCCATCTATCAGTGGCTCAATGTCCTCTGCCAATACCTTTACCTTTTCCCTCAATCTTCGGGAACCGTGTTGGCCAACATGGTGAGCTTCTGGGCACCCTAGATATTGGTTCCTCATCAAAGGGATCACTTGATGTCCATTCCATCCCTATGGCAGCTAGACTACGGTCTAGCAGTGCCGTCCTGCCATTTTTGGAAAATAGGTTGGGAGATCTTCGCAAGTTTGGGATTGCCAGAGGAGGACCTGGGGCAGAGTTACTTAGGTGTTGGGGATTCACAAAGGATGAATCAGAAGACATGGGGGAGACACTATCTAAAATGATAATGGAATTGAATCCTCACACTCAAACATCCTCAGATTCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Misat_Tub_SegII Tubulin_3

Protein Analysis

452

Amino Acids

48.85

Weight (kDa)

5.1

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_3 PF14881 87 - 219 2.6e-12 Tubulin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 893, 1082
AciI CCGC 1 cut(s) 488
AclWI GGATC 1 cut(s) 1120
AcoI YGGCCR 1 cut(s) 1060
AcsI RAATTY 2 cut(s) 106, 145
AcvI CACGTG 1 cut(s) 952
AcyI GRCGYC 1 cut(s) 894
AfaI GTAC 3 cut(s) 42, 128, 948
AfiI CCNNNNNNNGG 2 cut(s) 240, 307
AflIII ACRYGT 1 cut(s) 949
AgsI TTSAA 4 cut(s) 161, 501, 857, 1321
AhdI GACNNNNNGTC 1 cut(s) 865
AjnI CCWGG 2 cut(s) 129, 1231
AleI CACNNNNGTG 1 cut(s) 609
AluBI AGCT 5 cut(s) 478, 746, 800, 1075, 1145
AluI AGCT 5 cut(s) 478, 746, 800, 1075, 1145
Alw26I GTCTC 2 cut(s) 207, 1286
AlwI GGATC 1 cut(s) 1120
AoxI GGCC 4 cut(s) 671, 910, 977, 1060
ApaI GGGCCC 1 cut(s) 675
ApeKI GCWGC 3 cut(s) 623, 776, 1142
ApoI RAATTY 2 cut(s) 106, 145
AseI ATTAAT 1 cut(s) 927
AspLEI GCGC 1 cut(s) 896
AspS9I GGNCC 4 cut(s) 425, 671, 672, 1229
AsuHPI GGTGA 1 cut(s) 1082
AvaII GGWCC 2 cut(s) 425, 1229
BaeGI GKGCMC 2 cut(s) 675, 1085
BalI TGGCCA 1 cut(s) 1062
BanI GGYRCC 2 cut(s) 893, 1082
BanII GRGCYC 1 cut(s) 675
BbrPI CACGTG 1 cut(s) 952
BbsI GAAGAC 2 cut(s) 864, 1287
BbvI GCAGC 3 cut(s) 610, 763, 1154
BccI CCATC 3 cut(s) 52, 999, 1139
BceAI ACGGC 1 cut(s) 1151
BciT130I CCWGG 2 cut(s) 131, 1233
BcoDI GTCTC 2 cut(s) 207, 1286
BfaI CTAG 4 cut(s) 747, 1088, 1146, 1158
BfmI CTRYAG 1 cut(s) 607
BfoI RGCGCY 1 cut(s) 897
BglII AGATCT 1 cut(s) 1198
BisI GCNGC 3 cut(s) 624, 777, 1143
BlsI GCNGC 3 cut(s) 625, 778, 1144
Bme1390I CCNGG 2 cut(s) 131, 1233
Bme18I GGWCC 2 cut(s) 425, 1229
BmeRI GACNNNNNGTC 1 cut(s) 865
BmgT120I GGNCC 4 cut(s) 425, 671, 672, 1229
BmiI GGNNCC 8 cut(s) 426, 575, 663, 673, 895, 1052, 1084, 1099
BmrFI CCNGG 2 cut(s) 131, 1233
BmrI ACTGGG 1 cut(s) 669
BmsI GCATC 5 cut(s) 106, 475, 575, 763, 929
BmuI ACTGGG 1 cut(s) 669
BoxI GACNNNNGTC 1 cut(s) 1153
BpiI GAAGAC 2 cut(s) 864, 1287
BpmI CTGGAG 1 cut(s) 389
BpuEI CTTGAG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 952
BsaHI GRCGYC 1 cut(s) 894
BsaJI CCNNGG 3 cut(s) 889, 898, 1232
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 307
Bse1I ACTGG 3 cut(s) 316, 533, 675
Bse3DI GCAATG 2 cut(s) 621, 786
BseBI CCWGG 2 cut(s) 131, 1233
BseDI CCNNGG 3 cut(s) 889, 898, 1232
BseGI GGATG 6 cut(s) 70, 778, 823, 1131, 1276, 1337
BseLI CCNNNNNNNGG 2 cut(s) 240, 307
BseMI GCAATG 2 cut(s) 621, 786
BseMII CTCAG 3 cut(s) 348, 542, 1356
BseNI ACTGG 3 cut(s) 316, 533, 675
BseRI GAGGAG 2 cut(s) 566, 1239
BseSI GKGCMC 2 cut(s) 675, 1085
BseXI GCAGC 3 cut(s) 610, 763, 1154
BsgI GTGCAG 2 cut(s) 200, 699
BshFI GGCC 4 cut(s) 673, 912, 979, 1062
BshNI GGYRCC 2 cut(s) 893, 1082
BslI CCNNNNNNNGG 2 cut(s) 240, 307
BsmAI GTCTC 2 cut(s) 207, 1286
BsnI GGCC 4 cut(s) 673, 912, 979, 1062
Bsp120I GGGCCC 1 cut(s) 671
Bsp1286I GDGCHC 2 cut(s) 675, 1085
Bsp143I GATC 2 cut(s) 1112, 1198
BspACI CCGC 1 cut(s) 488
BspANI GGCC 4 cut(s) 673, 912, 979, 1062
BspCNI CTCAG 3 cut(s) 347, 543, 1355
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 8 cut(s) 426, 575, 663, 673, 895, 1052, 1084, 1099
BspMAI CTGCAG 1 cut(s) 611
BspPI GGATC 1 cut(s) 1120
BspQI GCTCTTC 1 cut(s) 129
BspT107I GGYRCC 2 cut(s) 893, 1082
BsrDI GCAATG 2 cut(s) 621, 786
BsrI ACTGG 3 cut(s) 316, 533, 675
BssECI CCNNGG 3 cut(s) 889, 898, 1232
BssMI GATC 2 cut(s) 1112, 1198
BssNI GRCGYC 1 cut(s) 894
BssT1I CCWWGG 1 cut(s) 898
Bst2UI CCWGG 2 cut(s) 131, 1233
Bst4CI ACNGT 6 cut(s) 19, 157, 712, 867, 1055, 1155
Bst6I CTCTTC 3 cut(s) 129, 219, 285
BstACI GRCGYC 1 cut(s) 894
BstAPI GCANNNNNTGC 1 cut(s) 620
BstBAI YACGTR 1 cut(s) 952
BstC8I GCNNGC 6 cut(s) 647, 744, 786, 914, 918, 966
BstDEI CTNAG 4 cut(s) 334, 551, 1247, 1342
BstF5I GGATG 6 cut(s) 70, 778, 823, 1131, 1276, 1337
BstH2I RGCGCY 1 cut(s) 897
BstHHI GCGC 1 cut(s) 896
BstKTI GATC 2 cut(s) 1115, 1201
BstMAI GTCTC 2 cut(s) 207, 1286
BstMBI GATC 2 cut(s) 1112, 1198
BstMWI GCNNNNNNNGC 3 cut(s) 620, 701, 785
BstNI CCWGG 2 cut(s) 131, 1233
BstNSI RCATGY 3 cut(s) 649, 854, 968
BstPAI GACNNNNGTC 1 cut(s) 1153
BstSCI CCNGG 2 cut(s) 129, 1231
BstSFI CTRYAG 1 cut(s) 607
BstSLI GKGCMC 2 cut(s) 675, 1085
BstV1I GCAGC 3 cut(s) 610, 763, 1154
BstV2I GAAGAC 2 cut(s) 864, 1287
BstX2I RGATCY 1 cut(s) 1198
BstXI CCANNNNNNTGG 1 cut(s) 1138
BstYI RGATCY 1 cut(s) 1198
BsuRI GGCC 4 cut(s) 673, 912, 979, 1062
BtsCI GGATG 6 cut(s) 70, 778, 823, 1131, 1276, 1337
BtsI GCAGTG 3 cut(s) 604, 616, 1168
BtsIMutI CAGTG 8 cut(s) 178, 548, 604, 616, 682, 827, 1005, 1168
Cac8I GCNNGC 6 cut(s) 647, 744, 786, 914, 918, 966
CciI TCATGA 1 cut(s) 481
CfoI GCGC 1 cut(s) 896
Cfr13I GGNCC 4 cut(s) 425, 671, 672, 1229
Csp6I GTAC 3 cut(s) 41, 127, 947
CviQI GTAC 3 cut(s) 41, 127, 947
DdeI CTNAG 4 cut(s) 334, 551, 1247, 1342
DinI GGCGCC 1 cut(s) 895
DpnI GATC 2 cut(s) 1114, 1200
DpnII GATC 2 cut(s) 1112, 1198
DriI GACNNNNNGTC 1 cut(s) 865
EaeI YGGCCR 1 cut(s) 1060
Eam1104I CTCTTC 3 cut(s) 129, 219, 285
Eam1105I GACNNNNNGTC 1 cut(s) 865
EarI CTCTTC 3 cut(s) 129, 219, 285
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 1 cut(s) 675
Eco47I GGWCC 2 cut(s) 425, 1229
Eco72I CACGTG 1 cut(s) 952
EcoO109I RGGNCCY 2 cut(s) 425, 1229
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 129, 1231
EcoT14I CCWWGG 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 675
EgeI GGCGCC 1 cut(s) 895
EheI GGCGCC 1 cut(s) 895
ErhI CCWWGG 1 cut(s) 898
FalI AAGNNNNNCTT 2 cut(s) 493, 525
Fnu4HI GCNGC 3 cut(s) 624, 777, 1143
FokI GGATG 6 cut(s) 77, 785, 810, 1118, 1283, 1324
FriOI GRGCYC 1 cut(s) 675
Fsp4HI GCNGC 3 cut(s) 624, 777, 1143
FspBI CTAG 4 cut(s) 747, 1088, 1146, 1158
GlaI GCGC 1 cut(s) 895
GluI GCNGC 3 cut(s) 624, 777, 1143
GsuI CTGGAG 1 cut(s) 389
HaeII RGCGCY 1 cut(s) 897
HaeIII GGCC 4 cut(s) 673, 912, 979, 1062
HhaI GCGC 1 cut(s) 896
Hin1I GRCGYC 1 cut(s) 894
Hin6I GCGC 1 cut(s) 894
HinP1I GCGC 1 cut(s) 894
HindIII AAGCTT 1 cut(s) 476
HphI GGTGA 1 cut(s) 1082
Hpy166II GTNNAC 3 cut(s) 248, 521, 949
Hpy188I TCNGA 9 cut(s) 91, 102, 208, 351, 656, 847, 1279, 1345, 1351
Hpy188III TCNNGA 7 cut(s) 12, 48, 336, 368, 482, 770, 1048
Hpy8I GTNNAC 3 cut(s) 248, 521, 949
HpyAV CCTTC 2 cut(s) 555, 801
HpyCH4III ACNGT 6 cut(s) 19, 157, 712, 867, 1055, 1155
HpyCH4IV ACGT 1 cut(s) 951
HpyF10VI GCNNNNNNNGC 3 cut(s) 620, 701, 785
HpyF3I CTNAG 4 cut(s) 334, 551, 1247, 1342
HpySE526I ACGT 1 cut(s) 951
Hsp92I GRCGYC 1 cut(s) 894
HspAI GCGC 1 cut(s) 894
KasI GGCGCC 1 cut(s) 893
Kzo9I GATC 2 cut(s) 1112, 1198
LguI GCTCTTC 1 cut(s) 129
LmnI GCTCC 1 cut(s) 579
Lsp1109I GCAGC 3 cut(s) 610, 763, 1154
LweI GCATC 5 cut(s) 106, 475, 575, 763, 929
MaeI CTAG 4 cut(s) 747, 1088, 1146, 1158
MaeII ACGT 1 cut(s) 951
MaeIII GTNAC 3 cut(s) 266, 296, 1242
MalI GATC 2 cut(s) 1114, 1200
MboI GATC 2 cut(s) 1112, 1198
MboII GAAGA 9 cut(s) 146, 236, 269, 302, 501, 869, 1036, 1193, 1292
MflI RGATCY 1 cut(s) 1198
MhlI GDGCHC 2 cut(s) 675, 1085
MlsI TGGCCA 1 cut(s) 1062
MluCI AATT 4 cut(s) 106, 145, 924, 1316
MluNI TGGCCA 1 cut(s) 1062
Mly113I GGCGCC 1 cut(s) 894
MlyI GAGTC 3 cut(s) 326, 474, 677
Mox20I TGGCCA 1 cut(s) 1062
MscI TGGCCA 1 cut(s) 1062
MseI TTAA 4 cut(s) 585, 714, 927, 1354
MslI CAYNNNNRTG 2 cut(s) 609, 1136
Msp20I TGGCCA 1 cut(s) 1062
MspR9I CCNGG 2 cut(s) 131, 1233
MvaI CCWGG 2 cut(s) 131, 1233
MwoI GCNNNNNNNGC 3 cut(s) 620, 701, 785
NarI GGCGCC 1 cut(s) 894
NdeII GATC 2 cut(s) 1112, 1198
NlaIV GGNNCC 8 cut(s) 426, 575, 663, 673, 895, 1052, 1084, 1099
NmeAIII GCCGAG 1 cut(s) 870
NmuCI GTSAC 2 cut(s) 266, 296
NspI RCATGY 3 cut(s) 649, 854, 968
OliI CACNNNNGTG 1 cut(s) 609
PaeI GCATGC 2 cut(s) 649, 968
PagI TCATGA 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 129
PfeI GAWTC 7 cut(s) 230, 443, 875, 1260, 1274, 1321, 1346
PkrI GCNGC 3 cut(s) 625, 778, 1144
PleI GAGTC 3 cut(s) 326, 473, 677
PluTI GGCGCC 1 cut(s) 897
PmaCI CACGTG 1 cut(s) 952
PmlI CACGTG 1 cut(s) 952
PpsI GAGTC 3 cut(s) 326, 473, 677
Ppu21I YACGTR 1 cut(s) 952
PpuMI RGGWCCY 2 cut(s) 425, 1229
PshAI GACNNNNGTC 1 cut(s) 1153
PshBI ATTAAT 1 cut(s) 927
Psp5II RGGWCCY 2 cut(s) 425, 1229
Psp6I CCWGG 2 cut(s) 129, 1231
PspCI CACGTG 1 cut(s) 952
PspGI CCWGG 2 cut(s) 129, 1231
PspN4I GGNNCC 8 cut(s) 426, 575, 663, 673, 895, 1052, 1084, 1099
PspOMI GGGCCC 1 cut(s) 671
PspPI GGNCC 4 cut(s) 425, 671, 672, 1229
PspPPI RGGWCCY 2 cut(s) 425, 1229
PstI CTGCAG 1 cut(s) 611
PsuI RGATCY 1 cut(s) 1198
RsaI GTAC 3 cut(s) 42, 128, 948
RsaNI GTAC 3 cut(s) 41, 127, 947
RseI CAYNNNNRTG 2 cut(s) 609, 1136
SapI GCTCTTC 1 cut(s) 129
SaqAI TTAA 4 cut(s) 585, 714, 927, 1354
SatI GCNGC 3 cut(s) 624, 777, 1143
Sau3AI GATC 2 cut(s) 1112, 1198
Sau96I GGNCC 4 cut(s) 425, 671, 672, 1229
SchI GAGTC 3 cut(s) 326, 474, 677
ScrFI CCNGG 2 cut(s) 131, 1233
SduI GDGCHC 2 cut(s) 675, 1085
SfaNI GCATC 5 cut(s) 106, 475, 575, 763, 929
SfcI CTRYAG 1 cut(s) 607
SfoI GGCGCC 1 cut(s) 895
SinI GGWCC 2 cut(s) 425, 1229
SmiMI CAYNNNNRTG 2 cut(s) 609, 1136
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
SphI GCATGC 2 cut(s) 649, 968
Sse9I AATT 4 cut(s) 106, 145, 924, 1316
SsiI CCGC 1 cut(s) 488
SspDI GGCGCC 1 cut(s) 893
SspMI CTAG 4 cut(s) 747, 1088, 1146, 1158
StyD4I CCNGG 2 cut(s) 129, 1231
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 6 cut(s) 19, 157, 712, 867, 1055, 1155
TaiI ACGT 1 cut(s) 954
TasI AATT 4 cut(s) 106, 145, 924, 1316
TfiI GAWTC 7 cut(s) 230, 443, 875, 1260, 1274, 1321, 1346
Tru1I TTAA 4 cut(s) 585, 714, 927, 1354
Tru9I TTAA 4 cut(s) 585, 714, 927, 1354
TscAI CASTG 8 cut(s) 178, 555, 611, 616, 682, 834, 1005, 1168
TseFI GTSAC 2 cut(s) 266, 296
TseI GCWGC 3 cut(s) 623, 776, 1142
Tsp45I GTSAC 2 cut(s) 266, 296
TspDTI ATGAA 9 cut(s) 24, 274, 310, 399, 470, 483, 606, 945, 1287
TspRI CASTG 8 cut(s) 178, 555, 611, 616, 682, 834, 1005, 1168
VpaK11BI GGWCC 2 cut(s) 425, 1229
VspI ATTAAT 1 cut(s) 927
XapI RAATTY 2 cut(s) 106, 145
XceI RCATGY 3 cut(s) 649, 854, 968
XspI CTAG 4 cut(s) 747, 1088, 1146, 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.