Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005482

Physical Location & Seq

Genomic Coordinates Forward (+)

14308818 .. 14314076

Gene Structure 5259 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1166800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGAGAGAACTCGTCACTCTTCAAGTTGGAAGCTTTGCAAACTATGTGGGCTCCCATTTCTGGAACTTCCAGGATGAACTACTCGGGTTGGCGGAGGACGCTTACGCCGATCCGGTGTTCAAGAATCAATCTTTGAACATGGACGTGCTCTACCGCTCTGGTGAGACGCACCAGGGCACTGTGACTTACACACCCCGCCTCGTTTCGGTTGATCTTCAAGGGTCCCTCGGGTCTATGAGTTCACGCGGCACATTGTATAATGAGGGTTCATCTGCATCGTCAAATATTTTCACATGGGGTGGTAATGTTTCTACTCAAGCCGCTGAGCCTCATAAGAAGAATTTGTTCTTACAAAGTTTGTACGAGGAAGAGGAGGGGGATTTTTTGACTCTTCAAAGTGGTGTCAGTGGTGGGGAGGAGAGTTCTCAAAGAGAAATTCATGACAGCGAAATAGTGGAGAGTTTAGAAAATGGGGTCCGGTACTGGACTGATTTTTCAAAAGTTCACTACCATCCCCAGAGTTTATATGAATTGAGTGGATTGTGGGTGGATCCTCAGAAATTTGACAATTACGGGATTGGAAGAGAATCCTTATCTGCGGGTTTACAGGGAGAAGAAGTTAGTGAGAGGCTTCGCTTTTTTGTAGAAGAGTGTGACCATATTCAGGGTTTTCAATTCATCGTTGATGACTCGGGAGGTTTCTCTCCTCTAGCTGTGGACGTTCTGGAGAGTATTGCTGATGAATACACAAATGCTCCTGTGATGCTTTATGCTGTGCGTGGTTCAGGTTCTTCCATGGACCCCAGAAGCCAGAAGCAGAGAGTCAGTAGGAAGCTTCACGATGCAGTTTCGTTTTCAATGCTGTCATCCTTATGCAAACTGATTGTTCCAGTTGGGCTACCCTCATTGAGTACAAGCAAAGCTTCAACCTTTCTCAGCATCAACAATGAAAGGCCTTATCACTGCAGTGCAGCTTACGCTGCCACCCTACATTCTGTTAGTCTCCCTTTTCGAATGGAACCACTTGGGCCCACTGGAGACTCATCTTATGCTTCTGGTGCTGTGACTGTTAGTGAGGTTGTGCAAATCTTATCAGGGCAAGCTAGGCAAAATATGGTTGCCATTCTGGATGCTGCAATGCCATCCCCTTCTTTAGCTGGGAATTCGCTTGGTGAGACTTTATTAAGGAATTTGCAGCCATTGACACCAGAGACAGCAGAAGATGTTGAGGACTTACAGTCAGTAGAATCCATGACAGTCCATGGGGCCCTAGGATCAGGAGGTCATCGGGCATCAATTTCTGAAGTTAAGGATACAGTTCACGCTGCCTATGAGCATGCAATAACAAGGCCGATCTACTGCCATCTGTCAGTGGCTCAATGTCCTCTTCCAATACCTTTGCCTTTTCCTTCAATTTTTGGCAACCTTGTTGGCCAACATGGTGAACTTTTGAGCACGCCAATTGTTGGTTCCCCCACAAGGGGATCACTTGATGTTCATTCCATCCCTATGGCAGCTAGACTACGGTCTAGCAGTGCTGTCTTGCCATTTTTGGAGAATAGATTGGGTGGCCTCCGTAGCTGTGGGATTGCCAGAGGAGCTCCTGGGGCGGAGTTAGTTAGAAGTTGGGGCTTTGCAAAGGATGAATTAGAAGACATGGGTGAGATGCTGTCTAAAATGATCGCGACACTGGATCCGCATTCTCAAATGTGGAGATACGTGGAAGTGCGGAAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Misat_Tub_SegII Tubulin_3

Protein Analysis

580

Amino Acids

63.01

Weight (kDa)

5.03

Isoelectric Point (pI)

49.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Misat_Tub_SegII	PF10644	2 - 121	2.6e-36	Misato Segment II tubulin-like domain
Tubulin_3	PF14881	157 - 338	5.8e-23	Tubulin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005482)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37190 AT4G37190
fragaria_vesca	FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_5g33502
malus_domestica	MD02G1166800.v1.1 MD15G1279300.v1.1
prunus_persica	Prupe.7G138000_v2.0.a1 Prupe.7G138000_v2.0.a1 Prupe.7G138000_v2.0.a1
pyrus_communis	pycom15g24210 pycom15g24220
rosa_chinensis	RchiOBHm_Chr2g0104711
rosa_laevigata	RLG00000017353
rosa_multiflora	Rmu_co8514121.1_g000001 Rmu_sc0002316.1_g000019
rosa_roxburghii	Rroxscaffold_2G00138810
rosa_rugosa	Rorug02G0121900
rosa_samantha	Rh2AG172200 Rh2BG179800 Rh2CG177300 Rh2DG178500
rosa_wichuraiana	Rw0G008630 Rw2G013480 Rw2G013540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1466
AccBSI	CCGCTC	1 cut(s)	156
AccII	CGCG	2 cut(s)	246, 1685
AciI	CCGC	9 cut(s)	92, 154, 196, 246, 321, 599, 1610, 1697, 1729
AclWI	GGATC	7 cut(s)	104, 545, 558, 1282, 1492, 1688, 1701
AcoI	YGGCCR	1 cut(s)	1432
AcsI	RAATTY	5 cut(s)	340, 435, 560, 1162, 1189
AcuI	CTGAAG	1 cut(s)	1323
AfaI	GTAC	3 cut(s)	362, 482, 913
AfiI	CCNNNNNNNGG	7 cut(s)	60, 205, 664, 1466, 1479, 1480, 1481
AhdI	GACNNNNNGTC	1 cut(s)	1237
AjiI	CACGTC	1 cut(s)	145
AjnI	CCWGG	3 cut(s)	69, 171, 1603
AjuI	GAANNNNNNNTTGG	2 cut(s)	1453, 1485
AleI	CACNNNNGTG	1 cut(s)	966
Alw21I	GWGCWC	3 cut(s)	150, 1457, 1603
Alw26I	GTCTC	5 cut(s)	158, 1007, 1032, 1169, 1205
AlwI	GGATC	7 cut(s)	104, 545, 558, 1282, 1492, 1688, 1701
Ama87I	CYCGRG	3 cut(s)	83, 227, 691
AoxI	GGCC	6 cut(s)	953, 1028, 1266, 1349, 1432, 1570
ApaI	GGGCCC	2 cut(s)	1032, 1270
ApeKI	GCWGC	6 cut(s)	971, 980, 1133, 1195, 1325, 1514
ApoI	RAATTY	5 cut(s)	340, 435, 560, 1162, 1189
Asp700I	GAANNNNTTC	1 cut(s)	344
AspA2I	CCTAGG	1 cut(s)	1270
AspS9I	GGNCC	7 cut(s)	222, 475, 799, 1028, 1029, 1266, 1267
AsuHPI	GGTGA	4 cut(s)	173, 1184, 1454, 1673
AsuII	TTCGAA	1 cut(s)	1012
AvaI	CYCGRG	3 cut(s)	83, 227, 691
AvaII	GGWCC	3 cut(s)	222, 475, 799
AvrII	CCTAGG	1 cut(s)	1270
BaeGI	GKGCMC	3 cut(s)	179, 1032, 1270
BalI	TGGCCA	1 cut(s)	1434
BamHI	GGATCC	2 cut(s)	550, 1693
BanII	GRGCYC	4 cut(s)	53, 1032, 1270, 1603
BbsI	GAAGAC	1 cut(s)	1659
Bbv12I	GWGCWC	3 cut(s)	150, 1457, 1603
BbvI	GCAGC	6 cut(s)	967, 983, 1120, 1207, 1312, 1526
BccI	CCATC	4 cut(s)	519, 1150, 1371, 1511
BciT130I	CCWGG	3 cut(s)	71, 173, 1605
BciVI	GTATCC	1 cut(s)	1306
BcoDI	GTCTC	5 cut(s)	158, 1007, 1032, 1169, 1205
BfaI	CTAG	5 cut(s)	710, 1104, 1271, 1518, 1530
BfmI	CTRYAG	1 cut(s)	964
BfuI	GTATCC	1 cut(s)	1306
BisI	GCNGC	8 cut(s)	247, 321, 972, 981, 1134, 1196, 1326, 1515
BlnI	CCTAGG	1 cut(s)	1270
BlpI	GCTNAGC	1 cut(s)	324
BlsI	GCNGC	8 cut(s)	248, 322, 973, 982, 1135, 1197, 1327, 1516
Bme1390I	CCNGG	3 cut(s)	71, 173, 1605
Bme18I	GGWCC	3 cut(s)	222, 475, 799
BmeRI	GACNNNNNGTC	1 cut(s)	1237
BmeT110I	CYCGRG	3 cut(s)	83, 227, 691
BmgBI	CACGTC	1 cut(s)	145
BmgT120I	GGNCC	7 cut(s)	222, 475, 799, 1028, 1029, 1266, 1267
BmrFI	CCNGG	3 cut(s)	71, 173, 1605
BmsI	GCATC	7 cut(s)	284, 753, 832, 948, 1120, 1301, 1656
BoxI	GACNNNNGTC	1 cut(s)	1525
BpiI	GAAGAC	1 cut(s)	1659
BpmI	CTGGAG	2 cut(s)	746, 1056
Bpu1102I	GCTNAGC	1 cut(s)	324
Bpu14I	TTCGAA	1 cut(s)	1012
BpuEI	CTTGAG	1 cut(s)	300
BsaAI	YACGTR	1 cut(s)	1720
BsaJI	CCNNGG	6 cut(s)	172, 226, 795, 1261, 1270, 1604
BsaWI	WCCGGW	2 cut(s)	112, 477
BsaXI	ACNNNNNCTCC	4 cut(s)	603, 633, 739, 769
Bsc4I	CCNNNNNNNGG	7 cut(s)	60, 205, 664, 1466, 1479, 1480, 1481
Bse1I	ACTGG	4 cut(s)	488, 890, 1039, 1695
Bse3DI	GCAATG	1 cut(s)	1143
BseBI	CCWGG	3 cut(s)	71, 173, 1605
BseDI	CCNNGG	6 cut(s)	172, 226, 795, 1261, 1270, 1604
BseGI	GGATG	7 cut(s)	79, 511, 866, 1135, 1142, 1503, 1648
BseLI	CCNNNNNNNGG	7 cut(s)	60, 205, 664, 1466, 1479, 1480, 1481
BseMI	GCAATG	1 cut(s)	1143
BseMII	CTCAG	3 cut(s)	315, 569, 949
BseNI	ACTGG	4 cut(s)	488, 890, 1039, 1695
BseRI	GAGGAG	4 cut(s)	386, 431, 696, 1611
BseSI	GKGCMC	3 cut(s)	179, 1032, 1270
BseXI	GCAGC	6 cut(s)	967, 983, 1120, 1207, 1312, 1526
BseYI	CCCAGC	1 cut(s)	1157
BsgI	GTGCAG	1 cut(s)	990
Bsh1236I	CGCG	2 cut(s)	246, 1685
BshFI	GGCC	6 cut(s)	955, 1030, 1268, 1351, 1434, 1572
BsiHKAI	GWGCWC	3 cut(s)	150, 1457, 1603
BsiHKCI	CYCGRG	3 cut(s)	83, 227, 691
BsiSI	CCGG	2 cut(s)	113, 478
BslFI	GGGAC	1 cut(s)	208
BslI	CCNNNNNNNGG	7 cut(s)	60, 205, 664, 1466, 1479, 1480, 1481
BsmAI	GTCTC	5 cut(s)	158, 1007, 1032, 1169, 1205
BsmBI	CGTCTC	1 cut(s)	158
BsmFI	GGGAC	1 cut(s)	208
BsmI	GAATGC	1 cut(s)	1699
BsnI	GGCC	6 cut(s)	955, 1030, 1268, 1351, 1434, 1572
BsoBI	CYCGRG	3 cut(s)	83, 227, 691
Bsp119I	TTCGAA	1 cut(s)	1012
Bsp120I	GGGCCC	2 cut(s)	1028, 1266
Bsp1286I	GDGCHC	7 cut(s)	53, 150, 179, 1032, 1270, 1457, 1603
Bsp143I	GATC	8 cut(s)	109, 211, 550, 1274, 1353, 1484, 1680, 1693
Bsp1720I	GCTNAGC	1 cut(s)	324
Bsp19I	CCATGG	2 cut(s)	795, 1261
Bsp68I	TCGCGA	1 cut(s)	1685
BspACI	CCGC	9 cut(s)	92, 154, 196, 246, 321, 599, 1610, 1697, 1729
BspANI	GGCC	6 cut(s)	955, 1030, 1268, 1351, 1434, 1572
BspCNI	CTCAG	3 cut(s)	316, 568, 948
BspFNI	CGCG	2 cut(s)	246, 1685
BspHI	TCATGA	1 cut(s)	439
BspMAI	CTGCAG	1 cut(s)	968
BspPI	GGATC	7 cut(s)	104, 545, 558, 1282, 1492, 1688, 1701
BspT104I	TTCGAA	1 cut(s)	1012
BsrBI	CCGCTC	1 cut(s)	156
BsrDI	GCAATG	1 cut(s)	1143
BsrI	ACTGG	4 cut(s)	488, 890, 1039, 1695
BssECI	CCNNGG	6 cut(s)	172, 226, 795, 1261, 1270, 1604
BssMI	GATC	8 cut(s)	109, 211, 550, 1274, 1353, 1484, 1680, 1693
BssT1I	CCWWGG	3 cut(s)	795, 1261, 1270
Bst2UI	CCWGG	3 cut(s)	71, 173, 1605
Bst4CI	ACNGT	6 cut(s)	181, 1069, 1239, 1258, 1318, 1527
Bst6I	CTCTTC	6 cut(s)	24, 363, 396, 577, 642, 1392
BstBAI	YACGTR	1 cut(s)	1720
BstBI	TTCGAA	1 cut(s)	1012
BstC8I	GCNNGC	3 cut(s)	1101, 1338, 1457
BstDEI	CTNAG	3 cut(s)	324, 555, 935
BstDSI	CCRYGG	2 cut(s)	795, 1261
BstF5I	GGATG	7 cut(s)	79, 511, 866, 1135, 1142, 1503, 1648
BstFNI	CGCG	2 cut(s)	246, 1685
BstKTI	GATC	8 cut(s)	112, 214, 553, 1277, 1356, 1487, 1683, 1696
BstMAI	GTCTC	5 cut(s)	158, 1007, 1032, 1169, 1205
BstMBI	GATC	8 cut(s)	109, 211, 550, 1274, 1353, 1484, 1680, 1693
BstMWI	GCNNNNNNNGC	8 cut(s)	98, 977, 980, 1058, 1105, 1578, 1598, 1607
BstNI	CCWGG	3 cut(s)	71, 173, 1605
BstNSI	RCATGY	1 cut(s)	1340
BstPAI	GACNNNNGTC	1 cut(s)	1525
BstSCI	CCNGG	3 cut(s)	69, 171, 1603
BstSFI	CTRYAG	1 cut(s)	964
BstSLI	GKGCMC	3 cut(s)	179, 1032, 1270
BstUI	CGCG	2 cut(s)	246, 1685
BstV1I	GCAGC	6 cut(s)	967, 983, 1120, 1207, 1312, 1526
BstV2I	GAAGAC	1 cut(s)	1659
BstX2I	RGATCY	2 cut(s)	550, 1693
BstXI	CCANNNNNNTGG	1 cut(s)	1510
BstYI	RGATCY	2 cut(s)	550, 1693
BsuI	GTATCC	1 cut(s)	1306
BsuRI	GGCC	6 cut(s)	955, 1030, 1268, 1351, 1434, 1572
BtgI	CCRYGG	2 cut(s)	795, 1261
BtrI	CACGTC	1 cut(s)	145
BtsCI	GGATG	7 cut(s)	79, 511, 866, 1135, 1142, 1503, 1648
BtsI	GCAGTG	3 cut(s)	961, 973, 1540
BtsIMutI	CAGTG	8 cut(s)	177, 412, 961, 973, 1032, 1377, 1540, 1688
BtuMI	TCGCGA	1 cut(s)	1685
Cac8I	GCNNGC	3 cut(s)	1101, 1338, 1457
CciI	TCATGA	1 cut(s)	439
Cfr13I	GGNCC	7 cut(s)	222, 475, 799, 1028, 1029, 1266, 1267
CseI	GACGC	2 cut(s)	107, 175
Csp6I	GTAC	3 cut(s)	361, 481, 912
CspCI	CAANNNNNGTGG	2 cut(s)	27, 62
CviAII	CATG	9 cut(s)	139, 294, 440, 796, 1252, 1262, 1337, 1439, 1657
CviQI	GTAC	3 cut(s)	361, 481, 912
DdeI	CTNAG	3 cut(s)	324, 555, 935
DpnI	GATC	8 cut(s)	111, 213, 552, 1276, 1355, 1486, 1682, 1695
DpnII	GATC	8 cut(s)	109, 211, 550, 1274, 1353, 1484, 1680, 1693
DriI	GACNNNNNGTC	1 cut(s)	1237
EaeI	YGGCCR	1 cut(s)	1432
Eam1104I	CTCTTC	6 cut(s)	24, 363, 396, 577, 642, 1392
Eam1105I	GACNNNNNGTC	1 cut(s)	1237
EarI	CTCTTC	6 cut(s)	24, 363, 396, 577, 642, 1392
EciI	GGCGGA	2 cut(s)	107, 1625
Ecl136II	GAGCTC	1 cut(s)	1601
Eco130I	CCWWGG	3 cut(s)	795, 1261, 1270
Eco147I	AGGCCT	1 cut(s)	955
Eco24I	GRGCYC	4 cut(s)	53, 1032, 1270, 1603
Eco47I	GGWCC	3 cut(s)	222, 475, 799
Eco53kI	GAGCTC	1 cut(s)	1601
Eco57I	CTGAAG	1 cut(s)	1323
Eco88I	CYCGRG	3 cut(s)	83, 227, 691
EcoICRI	GAGCTC	1 cut(s)	1601
EcoO109I	RGGNCCY	3 cut(s)	222, 1266, 1267
EcoRI	GAATTC	1 cut(s)	1162
EcoRII	CCWGG	3 cut(s)	69, 171, 1603
EcoT14I	CCWWGG	3 cut(s)	795, 1261, 1270
EcoT38I	GRGCYC	4 cut(s)	53, 1032, 1270, 1603
ErhI	CCWWGG	3 cut(s)	795, 1261, 1270
Esp3I	CGTCTC	1 cut(s)	158
FaeI	CATG	9 cut(s)	142, 297, 443, 799, 1255, 1265, 1340, 1442, 1660
FalI	AAGNNNNNCTT	2 cut(s)	907, 939
FaqI	GGGAC	1 cut(s)	208
FatI	CATG	9 cut(s)	138, 293, 439, 795, 1251, 1261, 1336, 1438, 1656
FauI	CCCGC	2 cut(s)	203, 592
Fnu4HI	GCNGC	8 cut(s)	247, 321, 972, 981, 1134, 1196, 1326, 1515
FokI	GGATG	7 cut(s)	86, 498, 853, 1129, 1142, 1490, 1655
FriOI	GRGCYC	4 cut(s)	53, 1032, 1270, 1603
Fsp4HI	GCNGC	8 cut(s)	247, 321, 972, 981, 1134, 1196, 1326, 1515
FspBI	CTAG	5 cut(s)	710, 1104, 1271, 1518, 1530
GluI	GCNGC	8 cut(s)	247, 321, 972, 981, 1134, 1196, 1326, 1515
GsaI	CCCAGC	1 cut(s)	1161
GsuI	CTGGAG	2 cut(s)	746, 1056
HaeIII	GGCC	6 cut(s)	955, 1030, 1268, 1351, 1434, 1572
HapII	CCGG	2 cut(s)	113, 478
HgaI	GACGC	2 cut(s)	107, 175
Hin1II	CATG	9 cut(s)	142, 297, 443, 799, 1255, 1265, 1340, 1442, 1660
HindIII	AAGCTT	3 cut(s)	31, 833, 921
HinfI	GANTC	7 cut(s)	124, 388, 587, 689, 822, 1040, 1247
HpaII	CCGG	2 cut(s)	113, 478
HphI	GGTGA	4 cut(s)	173, 1184, 1454, 1673
Hpy166II	GTNNAC	6 cut(s)	242, 505, 605, 718, 1321, 1445
Hpy188I	TCNGA	2 cut(s)	558, 1303
Hpy188III	TCNNGA	9 cut(s)	61, 121, 440, 693, 725, 839, 1127, 1278, 1684
Hpy8I	GTNNAC	6 cut(s)	242, 505, 605, 718, 1321, 1445
HpyAV	CCTTC	2 cut(s)	1158, 1419
HpyCH4III	ACNGT	6 cut(s)	181, 1069, 1239, 1258, 1318, 1527
HpyCH4IV	ACGT	3 cut(s)	144, 720, 1719
HpyF10VI	GCNNNNNNNGC	8 cut(s)	98, 977, 980, 1058, 1105, 1578, 1598, 1607
HpyF3I	CTNAG	3 cut(s)	324, 555, 935
HpySE526I	ACGT	3 cut(s)	144, 720, 1719
Hsp92II	CATG	9 cut(s)	142, 297, 443, 799, 1255, 1265, 1340, 1442, 1660
KflI	GGGWCCC	1 cut(s)	222
Kzo9I	GATC	8 cut(s)	109, 211, 550, 1274, 1353, 1484, 1680, 1693
LmnI	GCTCC	4 cut(s)	56, 760, 1598, 1606
Lsp1109I	GCAGC	6 cut(s)	967, 983, 1120, 1207, 1312, 1526
LweI	GCATC	7 cut(s)	284, 753, 832, 948, 1120, 1301, 1656
MaeI	CTAG	5 cut(s)	710, 1104, 1271, 1518, 1530
MaeII	ACGT	3 cut(s)	144, 720, 1719
MaeIII	GTNAC	4 cut(s)	13, 181, 653, 1063
MalI	GATC	8 cut(s)	111, 213, 552, 1276, 1355, 1486, 1682, 1695
MbiI	CCGCTC	1 cut(s)	156
MboI	GATC	8 cut(s)	109, 211, 550, 1274, 1353, 1484, 1680, 1693
MfeI	CAATTG	1 cut(s)	1461
MflI	RGATCY	2 cut(s)	550, 1693
MhlI	GDGCHC	7 cut(s)	53, 150, 179, 1032, 1270, 1457, 1603
MlsI	TGGCCA	1 cut(s)	1434
MluNI	TGGCCA	1 cut(s)	1434
MlyI	GAGTC	4 cut(s)	382, 683, 831, 1034
MmeI	TCCRAC	1 cut(s)	7
Mox20I	TGGCCA	1 cut(s)	1434
MroXI	GAANNNNTTC	1 cut(s)	344
MscI	TGGCCA	1 cut(s)	1434
MseI	TTAA	2 cut(s)	1184, 1308
MslI	CAYNNNNRTG	4 cut(s)	143, 871, 966, 1508
Msp20I	TGGCCA	1 cut(s)	1434
MspA1I	CMGCKG	1 cut(s)	323
MspI	CCGG	2 cut(s)	113, 478
MspR9I	CCNGG	3 cut(s)	71, 173, 1605
MunI	CAATTG	1 cut(s)	1461
Mva1269I	GAATGC	1 cut(s)	1699
MvaI	CCWGG	3 cut(s)	71, 173, 1605
MvnI	CGCG	2 cut(s)	246, 1685
MwoI	GCNNNNNNNGC	8 cut(s)	98, 977, 980, 1058, 1105, 1578, 1598, 1607
NcoI	CCATGG	2 cut(s)	795, 1261
NdeII	GATC	8 cut(s)	109, 211, 550, 1274, 1353, 1484, 1680, 1693
NlaIII	CATG	9 cut(s)	142, 297, 443, 799, 1255, 1265, 1340, 1442, 1660
NmuCI	GTSAC	4 cut(s)	13, 181, 653, 1063
NruI	TCGCGA	1 cut(s)	1685
NspI	RCATGY	1 cut(s)	1340
NspV	TTCGAA	1 cut(s)	1012
OliI	CACNNNNGTG	1 cut(s)	966
PaeI	GCATGC	1 cut(s)	1340
PagI	TCATGA	1 cut(s)	439
PceI	AGGCCT	1 cut(s)	955
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PctI	GAATGC	1 cut(s)	1699
PdmI	GAANNNNTTC	1 cut(s)	344
PfeI	GAWTC	3 cut(s)	124, 587, 1247
PflMI	CCANNNNNTGG	1 cut(s)	1466
PfoI	TCCNGGA	1 cut(s)	69
PkrI	GCNGC	8 cut(s)	248, 322, 973, 982, 1135, 1197, 1327, 1516
PleI	GAGTC	4 cut(s)	382, 683, 830, 1034
PpsI	GAGTC	4 cut(s)	382, 683, 830, 1034
Ppu21I	YACGTR	1 cut(s)	1720
PpuMI	RGGWCCY	1 cut(s)	222
PshAI	GACNNNNGTC	1 cut(s)	1525
Psp124BI	GAGCTC	1 cut(s)	1603
Psp5II	RGGWCCY	1 cut(s)	222
Psp6I	CCWGG	3 cut(s)	69, 171, 1603
PspFI	CCCAGC	1 cut(s)	1157
PspGI	CCWGG	3 cut(s)	69, 171, 1603
PspOMI	GGGCCC	2 cut(s)	1028, 1266
PspPI	GGNCC	7 cut(s)	222, 475, 799, 1028, 1029, 1266, 1267
PspPPI	RGGWCCY	1 cut(s)	222
PstI	CTGCAG	1 cut(s)	968
PsuI	RGATCY	2 cut(s)	550, 1693
RruI	TCGCGA	1 cut(s)	1685
RsaI	GTAC	3 cut(s)	362, 482, 913
RsaNI	GTAC	3 cut(s)	361, 481, 912
RseI	CAYNNNNRTG	4 cut(s)	143, 871, 966, 1508
SacI	GAGCTC	1 cut(s)	1603
SaqAI	TTAA	2 cut(s)	1184, 1308
SatI	GCNGC	8 cut(s)	247, 321, 972, 981, 1134, 1196, 1326, 1515
Sau3AI	GATC	8 cut(s)	109, 211, 550, 1274, 1353, 1484, 1680, 1693
Sau96I	GGNCC	7 cut(s)	222, 475, 799, 1028, 1029, 1266, 1267
SchI	GAGTC	4 cut(s)	382, 683, 831, 1034
ScrFI	CCNGG	3 cut(s)	71, 173, 1605
SduI	GDGCHC	7 cut(s)	53, 150, 179, 1032, 1270, 1457, 1603
SfaNI	GCATC	7 cut(s)	284, 753, 832, 948, 1120, 1301, 1656
SfcI	CTRYAG	1 cut(s)	964
SfuI	TTCGAA	1 cut(s)	1012
SinI	GGWCC	3 cut(s)	222, 475, 799
SmiMI	CAYNNNNRTG	4 cut(s)	143, 871, 966, 1508
SmlI	CTYRAG	1 cut(s)	315
SmoI	CTYRAG	1 cut(s)	315
SphI	GCATGC	1 cut(s)	1340
SseBI	AGGCCT	1 cut(s)	955
SsiI	CCGC	9 cut(s)	92, 154, 196, 246, 321, 599, 1610, 1697, 1729
SspI	AATATT	1 cut(s)	286
SspMI	CTAG	5 cut(s)	710, 1104, 1271, 1518, 1530
SstI	GAGCTC	1 cut(s)	1603
StuI	AGGCCT	1 cut(s)	955
StyD4I	CCNGG	3 cut(s)	69, 171, 1603
StyI	CCWWGG	3 cut(s)	795, 1261, 1270
TaaI	ACNGT	6 cut(s)	181, 1069, 1239, 1258, 1318, 1527
TaiI	ACGT	3 cut(s)	147, 723, 1722
TaqI	TCGA	1 cut(s)	1012
TatI	WGTACW	1 cut(s)	911
TauI	GCSGC	2 cut(s)	249, 323
TfiI	GAWTC	3 cut(s)	124, 587, 1247
Tru1I	TTAA	2 cut(s)	1184, 1308
Tru9I	TTAA	2 cut(s)	1184, 1308
TscAI	CASTG	8 cut(s)	184, 412, 968, 973, 1039, 1377, 1540, 1695
TseFI	GTSAC	4 cut(s)	13, 181, 653, 1063
TseI	GCWGC	6 cut(s)	971, 980, 1133, 1195, 1325, 1514
Tsp45I	GTSAC	4 cut(s)	13, 181, 653, 1063
TspDTI	ATGAA	9 cut(s)	90, 258, 428, 543, 667, 756, 963, 1487, 1659
TspGWI	ACGGA	1 cut(s)	1565
TspRI	CASTG	8 cut(s)	184, 412, 968, 973, 1039, 1377, 1540, 1695
Van91I	CCANNNNNTGG	1 cut(s)	1466
VpaK11BI	GGWCC	3 cut(s)	222, 475, 799
XapI	RAATTY	5 cut(s)	340, 435, 560, 1162, 1189
XceI	RCATGY	1 cut(s)	1340
XmaJI	CCTAGG	1 cut(s)	1270
XmnI	GAANNNNTTC	1 cut(s)	344
XspI	CTAG	5 cut(s)	710, 1104, 1271, 1518, 1530

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.