Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0005482

Physical Location & Seq

Genomic Coordinates Reverse (-)

10506810 .. 10510428

Gene Structure 3619 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0121900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGGGTTGTTGTTACTCGAGAATCGAGAGACAAGAAATGGTGTCGAGATGTAAATCGAGAAAGAGGTACATGAAGCAGTTGGTCAAGGCAAGACAAGCTTGGTCGGCTTCACACTCCATGTACCTACGATCTCTCCGAAGCACAGGTGCTGCTTTGCTCCAATTTTCAAATGCCGAAACTACCCTCCACCACCAGCACCACCCAACCCAACACCAGTCCCACCACCTCAACAACCACACCCACCACTCCCCGCCGCCGCCTCAAACCCCACAGACTCCGCTTCAACCGCCTCCGCCTCCTCCGCGGAGCCCAAGCTCCGAGACCACGTGGACCTCCACAGCCTCCACCGCCCTGCCGCCTCCTCCGCCGCCGCCGCCGTCGTCCACGTGGGATTTCTGGGACCCTTTTGTGCCGGCTTCGTCTCGGTCGGTGACCGAGGAGGAATGGGAGGCTACGACCACCGCGTCGGAGGCGGTGGTTACGACTGTCACCGGGGCGGCGAGCACCGTGGCGCCGCCTTCGGTTGTTAGTGGGTTTTCGAAGGACAGTGAGGCTAGCTCGATGGCAATGGTGGTGTCCAGGAATGCTAAGGATCTGGTGGAGATCATTAAGGAGCTTGATGAGTATTTTCTTAAAGCTGCCGATGCCGGTGGCCATCTCTCGTTTCTCTTGGAAGTTCCCACTCCTGGATTTTCTTCTCAGATTAAAGGAGGTAAAGTATATAACCATGGTTGTAATTTGAATACTCCATCTACATGGACATGGGGTTCGAGTCCTAAACTCAATGGTTTCGGGAAAATGGGTTGCGACGAAATGGTGTTAAGTCATTTAGGCAGCGAAATTGGGACTGATGGTGTGGTACATAGCAGCCATTGTTCTACTGTGGAGAGGCTCTATGCTTGGGAAAAGAAACTATTCCAGGAGGTCAAGGAGGCAGAGACAATAAAGACAGAGCATGAGAAGAGGGTGTCAACATTGAAGAAGTTAGAGATGAAGAGGGCAGCAGACTATGTGAAGACTGAGAAGTCCAAGAAAGAGGTGGAAAAGTTGGAGTCACAAATGATGGTTTCTTCTCAGGCCATTGAGACAACTTCTGCTGAGATCATCAAGATAAGGGACACAGAGTTGTACCCTCAACTACTTGAGCTTGTCAAAGGATTGATGTGTATGTGGAGAAGCATGTATGAGTGCCACCAAGTCCAGAAGCATATAGTTCAGCAACTCAAATACCTTAACACCATACCATCTACTGAGCCCACGTCTGAGATTCACAGGCAATCAACTCTCCAGCTCGAGGTTCATGTCCAGCAATGGCACCAGTCCTTTTGCAACCTGGTCAGGGCTCAATGTGACTATATCCAGTCCCTTACAGGATGGCTCCGGCTTAGCCTCTTCCAGTTTAGGAGAAATCCTTTACCAAAAACAAGTCAAGAATCAAGAATTTACACGTTCTGTGAAGAATGGCACCATGCAGTTGATCGGATCCCAGACAAAGTAGCATCTGAAGGAATCAAGAGCTTTTTAACTGTAGTGCATGCCATTGTAGTTCAACAAGCCGAAGAAAACAAGCAAAAAAAGAAGTCAGAATCTGCGTTTAAAGAGCTCGAGAGGAAGTCAAGTCAACTTAGGTCACTTGAGTCCAAACATGGCCCTTTCTCAATGCTAGATTCCTCTAGCTCTACTGCAAAGAACCCAGTCGCTGATAAGCGAGCAAAGGTGGAGAACTCGAGAGTAAAAGCAGAAGAGGAGAAGAACAAGCATGAAAAGTTTGTGAGCGTGACAAGATCCATGACCATAAATAACTTGCAGATGGGGTTTCCACATGTGTTTGAGTCGATGGTAGGCTTTTCAAGTGTGTGTATGCAGGCGTTCGAGTCATTATATAACCAAGCCAAAAGCGTTGATCAGGAGCTGGATGTTAAGAGGCTACTAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Misat_Tub_SegII Tubulin_3

Protein Analysis

645

Amino Acids

72.81

Weight (kDa)

8.54

Isoelectric Point (pI)

57.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF630	PF04783	1 - 59	1.6e-20	Protein of unknown function (DUF630)
DUF632	PF04782	199 - 512	2.2e-104	Protein of unknown function (DUF632)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005482)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37190 AT4G37190
fragaria_vesca	FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_5g33502
malus_domestica	MD02G1166800.v1.1 MD15G1279300.v1.1
prunus_persica	Prupe.7G138000_v2.0.a1 Prupe.7G138000_v2.0.a1 Prupe.7G138000_v2.0.a1
pyrus_communis	pycom15g24210 pycom15g24220
rosa_chinensis	RchiOBHm_Chr2g0104711
rosa_laevigata	RLG00000017353
rosa_multiflora	Rmu_co8514121.1_g000001 Rmu_sc0002316.1_g000019
rosa_roxburghii	Rroxscaffold_2G00138810
rosa_rugosa	Rorug02G0121900
rosa_samantha	Rh2AG172200 Rh2BG179800 Rh2CG177300 Rh2DG178500
rosa_wichuraiana	Rw0G008630 Rw2G013480 Rw2G013540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	463, 1024
AccB1I	GGYRCC	3 cut(s)	511, 1314, 1464
AccII	CGCG	2 cut(s)	304, 464
AclWI	GGATC	4 cut(s)	600, 1477, 1490, 1779
AcoI	YGGCCR	1 cut(s)	652
AcsI	RAATTY	1 cut(s)	1440
AcuI	CTGAAG	1 cut(s)	1524
AcvI	CACGTG	2 cut(s)	327, 387
AcyI	GRCGYC	1 cut(s)	512
AfaI	GTAC	4 cut(s)	68, 122, 861, 1130
AfiI	CCNNNNNNNGG	3 cut(s)	686, 1294, 1339
AflIII	ACRYGT	2 cut(s)	1446, 1822
AgsI	TTSAA	6 cut(s)	168, 284, 742, 979, 1550, 1851
AjiI	CACGTC	1 cut(s)	1260
AjnI	CCWGG	4 cut(s)	578, 685, 918, 1332
AloI	GAACNNNNNNTCC	2 cut(s)	751, 783
Alw21I	GWGCWC	2 cut(s)	506, 1605
Alw26I	GTCTC	5 cut(s)	22, 314, 426, 932, 1079
AlwI	GGATC	4 cut(s)	600, 1477, 1490, 1779
AlwNI	CAGNNNCTG	4 cut(s)	149, 1589, 1700, 1912
Ama87I	CYCGRG	4 cut(s)	16, 1292, 1604, 1726
AoxI	GGCC	3 cut(s)	652, 1077, 1648
ApeKI	GCWGC	5 cut(s)	149, 638, 834, 867, 1001
ApoI	RAATTY	1 cut(s)	1440
ArsI	GACNNNNNNTTYG	2 cut(s)	751, 783
Asp700I	GAANNNNTTC	1 cut(s)	914
AspLEI	GCGC	1 cut(s)	514
AspS9I	GGNCC	3 cut(s)	330, 400, 1649
AsuC2I	CCSGG	1 cut(s)	493
AsuHPI	GGTGA	2 cut(s)	442, 481
AsuII	TTCGAA	1 cut(s)	539
AsuNHI	GCTAGC	1 cut(s)	554
AvaI	CYCGRG	4 cut(s)	16, 1292, 1604, 1726
AvaII	GGWCC	2 cut(s)	330, 400
BalI	TGGCCA	1 cut(s)	654
BamHI	GGATCC	1 cut(s)	1482
BanI	GGYRCC	3 cut(s)	511, 1314, 1464
BanII	GRGCYC	4 cut(s)	311, 1257, 1345, 1605
BbrPI	CACGTG	2 cut(s)	327, 387
BbsI	GAAGAC	1 cut(s)	1022
Bbv12I	GWGCWC	2 cut(s)	506, 1605
BbvI	GCAGC	5 cut(s)	136, 625, 846, 879, 1013
BccI	CCATC	9 cut(s)	556, 663, 757, 845, 1057, 1252, 1368, 1804, 1831
BceAI	ACGGC	1 cut(s)	361
BciT130I	CCWGG	4 cut(s)	580, 687, 920, 1334
BclI	TGATCA	1 cut(s)	1903
BcnI	CCSGG	1 cut(s)	493
BcoDI	GTCTC	5 cut(s)	22, 314, 426, 932, 1079
BfaI	CTAG	3 cut(s)	555, 1664, 1674
BfmI	CTRYAG	1 cut(s)	1527
BfoI	RGCGCY	1 cut(s)	515
BlpI	GCTNAGC	1 cut(s)	1385
Bme1390I	CCNGG	5 cut(s)	493, 580, 687, 920, 1334
Bme18I	GGWCC	2 cut(s)	330, 400
BmeT110I	CYCGRG	4 cut(s)	16, 1292, 1604, 1726
BmgBI	CACGTC	1 cut(s)	1260
BmgT120I	GGNCC	3 cut(s)	330, 400, 1649
BmiI	GGNNCC	8 cut(s)	308, 401, 402, 513, 1316, 1379, 1466, 1484
BmrFI	CCNGG	5 cut(s)	493, 580, 687, 920, 1334
BmrI	ACTGGG	1 cut(s)	1688
BmsI	GCATC	2 cut(s)	634, 1508
BmtI	GCTAGC	1 cut(s)	558
BmuI	ACTGGG	1 cut(s)	1688
BpiI	GAAGAC	1 cut(s)	1022
BplI	GAGNNNNNCTC	2 cut(s)	542, 574
BpmI	CTGGAG	1 cut(s)	1271
Bpu10I	CCTNAGC	1 cut(s)	588
Bpu1102I	GCTNAGC	1 cut(s)	1385
Bpu14I	TTCGAA	1 cut(s)	539
BpuEI	CTTGAG	2 cut(s)	1163, 1655
BpuMI	CCSGG	1 cut(s)	493
BsaAI	YACGTR	2 cut(s)	327, 387
BsaBI	GATNNNNATC	1 cut(s)	52
BsaHI	GRCGYC	1 cut(s)	512
BsaI	GGTCTC	1 cut(s)	314
BsaJI	CCNNGG	5 cut(s)	302, 435, 492, 507, 727
BsaXI	ACNNNNNCTCC	4 cut(s)	117, 147, 702, 732
Bsc4I	CCNNNNNNNGG	3 cut(s)	686, 1294, 1339
Bse118I	RCCGGY	2 cut(s)	412, 647
Bse1I	ACTGG	5 cut(s)	214, 1318, 1360, 1396, 1694
Bse3DI	GCAATG	2 cut(s)	573, 1316
Bse8I	GATNNNNATC	1 cut(s)	52
BseBI	CCWGG	4 cut(s)	580, 687, 920, 1334
BseDI	CCNNGG	5 cut(s)	302, 435, 492, 507, 727
BseGI	GGATG	2 cut(s)	1379, 1921
BseJI	GATNNNNATC	1 cut(s)	52
BseLI	CCNNNNNNNGG	3 cut(s)	686, 1294, 1339
BseMI	GCAATG	2 cut(s)	573, 1316
BseMII	CTCAG	6 cut(s)	713, 1011, 1088, 1089, 1242, 1254
BseNI	ACTGG	5 cut(s)	214, 1318, 1360, 1396, 1694
BseRI	GAGGAG	4 cut(s)	288, 351, 452, 1760
BseXI	GCAGC	5 cut(s)	136, 625, 846, 879, 1013
Bsh1236I	CGCG	2 cut(s)	304, 464
Bsh1285I	CGRYCG	1 cut(s)	428
BshFI	GGCC	3 cut(s)	654, 1079, 1650
BshNI	GGYRCC	3 cut(s)	511, 1314, 1464
BsiEI	CGRYCG	1 cut(s)	428
BsiHKAI	GWGCWC	2 cut(s)	506, 1605
BsiHKCI	CYCGRG	4 cut(s)	16, 1292, 1604, 1726
BsiSI	CCGG	4 cut(s)	413, 492, 648, 1381
BslFI	GGGAC	5 cut(s)	202, 413, 859, 1130, 1348
BslI	CCNNNNNNNGG	3 cut(s)	686, 1294, 1339
BsmAI	GTCTC	5 cut(s)	22, 314, 426, 932, 1079
BsmBI	CGTCTC	1 cut(s)	426
BsmFI	GGGAC	5 cut(s)	202, 413, 859, 1130, 1348
BsmI	GAATGC	1 cut(s)	589
BsnI	GGCC	3 cut(s)	654, 1079, 1650
Bso31I	GGTCTC	1 cut(s)	314
BsoBI	CYCGRG	4 cut(s)	16, 1292, 1604, 1726
Bsp119I	TTCGAA	1 cut(s)	539
Bsp1286I	GDGCHC	5 cut(s)	311, 506, 1257, 1345, 1605
Bsp143I	GATC	8 cut(s)	128, 592, 603, 1101, 1477, 1482, 1784, 1903
Bsp1720I	GCTNAGC	1 cut(s)	1385
Bsp19I	CCATGG	1 cut(s)	727
BspANI	GGCC	3 cut(s)	654, 1079, 1650
BspCNI	CTCAG	6 cut(s)	712, 1012, 1087, 1090, 1243, 1255
BspFNI	CGCG	2 cut(s)	304, 464
BspLI	GGNNCC	8 cut(s)	308, 401, 402, 513, 1316, 1379, 1466, 1484
BspOI	GCTAGC	1 cut(s)	558
BspPI	GGATC	4 cut(s)	600, 1477, 1490, 1779
BspT104I	TTCGAA	1 cut(s)	539
BspT107I	GGYRCC	3 cut(s)	511, 1314, 1464
BspTNI	GGTCTC	1 cut(s)	314
BsrDI	GCAATG	2 cut(s)	573, 1316
BsrFI	RCCGGY	2 cut(s)	412, 647
BsrI	ACTGG	5 cut(s)	214, 1318, 1360, 1396, 1694
BssAI	RCCGGY	2 cut(s)	412, 647
BssECI	CCNNGG	5 cut(s)	302, 435, 492, 507, 727
BssMI	GATC	8 cut(s)	128, 592, 603, 1101, 1477, 1482, 1784, 1903
BssNI	GRCGYC	1 cut(s)	512
BssT1I	CCWWGG	1 cut(s)	727
Bst2UI	CCWGG	4 cut(s)	580, 687, 920, 1334
Bst4CI	ACNGT	5 cut(s)	487, 508, 548, 883, 1528
Bst6I	CTCTTC	4 cut(s)	956, 989, 1397, 1737
BstACI	GRCGYC	1 cut(s)	512
BstBAI	YACGTR	2 cut(s)	327, 387
BstBI	TTCGAA	1 cut(s)	539
BstC8I	GCNNGC	6 cut(s)	414, 502, 556, 1536, 1710, 1866
BstDEI	CTNAG	9 cut(s)	588, 699, 1020, 1074, 1098, 1251, 1263, 1385, 1625
BstDSI	CCRYGG	3 cut(s)	302, 507, 727
BstEII	GGTNACC	1 cut(s)	430
BstF5I	GGATG	2 cut(s)	1379, 1921
BstFNI	CGCG	2 cut(s)	304, 464
BstH2I	RGCGCY	1 cut(s)	515
BstHHI	GCGC	1 cut(s)	514
BstKTI	GATC	8 cut(s)	131, 595, 606, 1104, 1480, 1485, 1787, 1906
BstMAI	GTCTC	5 cut(s)	22, 314, 426, 932, 1079
BstMBI	GATC	8 cut(s)	128, 592, 603, 1101, 1477, 1482, 1784, 1903
BstMCI	CGRYCG	1 cut(s)	428
BstMWI	GCNNNNNNNGC	9 cut(s)	95, 104, 286, 301, 347, 364, 373, 470, 644
BstNI	CCWGG	4 cut(s)	580, 687, 920, 1334
BstNSI	RCATGY	3 cut(s)	1183, 1538, 1826
BstPI	GGTNACC	1 cut(s)	430
BstSCI	CCNGG	5 cut(s)	491, 578, 685, 918, 1332
BstSFI	CTRYAG	1 cut(s)	1527
BstUI	CGCG	2 cut(s)	304, 464
BstV1I	GCAGC	5 cut(s)	136, 625, 846, 879, 1013
BstV2I	GAAGAC	1 cut(s)	1022
BstX2I	RGATCY	3 cut(s)	592, 1482, 1784
BstXI	CCANNNNNNTGG	1 cut(s)	756
BstYI	RGATCY	3 cut(s)	592, 1482, 1784
BsuRI	GGCC	3 cut(s)	654, 1079, 1650
BtgI	CCRYGG	3 cut(s)	302, 507, 727
BtrI	CACGTC	1 cut(s)	1260
BtsCI	GGATG	2 cut(s)	1379, 1921
BtsIMutI	CAGTG	1 cut(s)	553
Cac8I	GCNNGC	6 cut(s)	414, 502, 556, 1536, 1710, 1866
CaiI	CAGNNNCTG	4 cut(s)	149, 1589, 1700, 1912
CfoI	GCGC	1 cut(s)	514
Cfr10I	RCCGGY	2 cut(s)	412, 647
Cfr13I	GGNCC	3 cut(s)	330, 400, 1649
Cfr42I	CCGCGG	1 cut(s)	305
CseI	GACGC	1 cut(s)	453
CsiI	ACCWGGT	1 cut(s)	1332
Csp6I	GTAC	4 cut(s)	67, 121, 860, 1129
CviQI	GTAC	4 cut(s)	67, 121, 860, 1129
DdeI	CTNAG	9 cut(s)	588, 699, 1020, 1074, 1098, 1251, 1263, 1385, 1625
DinI	GGCGCC	1 cut(s)	513
DpnI	GATC	8 cut(s)	130, 594, 605, 1103, 1479, 1484, 1786, 1905
DpnII	GATC	8 cut(s)	128, 592, 603, 1101, 1477, 1482, 1784, 1903
DraI	TTTAAA	1 cut(s)	1597
DrdI	GACNNNNNNGTC	2 cut(s)	463, 1024
DseDI	GACNNNNNNGTC	2 cut(s)	463, 1024
EaeI	YGGCCR	1 cut(s)	652
Eam1104I	CTCTTC	4 cut(s)	956, 989, 1397, 1737
EarI	CTCTTC	4 cut(s)	956, 989, 1397, 1737
EciI	GGCGGA	2 cut(s)	282, 354
Ecl136II	GAGCTC	1 cut(s)	1603
Eco130I	CCWWGG	1 cut(s)	727
Eco24I	GRGCYC	4 cut(s)	311, 1257, 1345, 1605
Eco31I	GGTCTC	1 cut(s)	314
Eco47I	GGWCC	2 cut(s)	330, 400
Eco53kI	GAGCTC	1 cut(s)	1603
Eco57I	CTGAAG	1 cut(s)	1524
Eco72I	CACGTG	2 cut(s)	327, 387
Eco88I	CYCGRG	4 cut(s)	16, 1292, 1604, 1726
Eco91I	GGTNACC	1 cut(s)	430
EcoICRI	GAGCTC	1 cut(s)	1603
EcoO109I	RGGNCCY	1 cut(s)	400
EcoO65I	GGTNACC	1 cut(s)	430
EcoRII	CCWGG	4 cut(s)	578, 685, 918, 1332
EcoT14I	CCWWGG	1 cut(s)	727
EcoT38I	GRGCYC	4 cut(s)	311, 1257, 1345, 1605
EgeI	GGCGCC	1 cut(s)	513
EheI	GGCGCC	1 cut(s)	513
ErhI	CCWWGG	1 cut(s)	727
Esp3I	CGTCTC	1 cut(s)	426
FalI	AAGNNNNNCTT	2 cut(s)	82, 114
FaqI	GGGAC	5 cut(s)	202, 413, 859, 1130, 1348
FauI	CCCGC	1 cut(s)	258
FbaI	TGATCA	1 cut(s)	1903
FokI	GGATG	2 cut(s)	1386, 1928
FriOI	GRGCYC	4 cut(s)	311, 1257, 1345, 1605
FspBI	CTAG	3 cut(s)	555, 1664, 1674
GlaI	GCGC	1 cut(s)	513
GsuI	CTGGAG	1 cut(s)	1271
HaeII	RGCGCY	1 cut(s)	515
HaeIII	GGCC	3 cut(s)	654, 1079, 1650
HapII	CCGG	4 cut(s)	413, 492, 648, 1381
HgaI	GACGC	1 cut(s)	453
HhaI	GCGC	1 cut(s)	514
Hin1I	GRCGYC	1 cut(s)	512
Hin6I	GCGC	1 cut(s)	512
HinP1I	GCGC	1 cut(s)	512
HincII	GTYRAC	2 cut(s)	972, 1622
HindII	GTYRAC	2 cut(s)	972, 1622
HindIII	AAGCTT	1 cut(s)	96
HpaII	CCGG	4 cut(s)	413, 492, 648, 1381
HphI	GGTGA	2 cut(s)	442, 481
Hpy166II	GTNNAC	4 cut(s)	330, 384, 972, 1622
Hpy188I	TCNGA	8 cut(s)	137, 319, 469, 702, 1264, 1482, 1504, 1585
Hpy8I	GTNNAC	4 cut(s)	330, 384, 972, 1622
Hpy99I	CGWCG	3 cut(s)	382, 469, 812
HpyAV	CCTTC	3 cut(s)	528, 535, 1499
HpyCH4III	ACNGT	5 cut(s)	487, 508, 548, 883, 1528
HpyCH4IV	ACGT	4 cut(s)	326, 386, 1259, 1448
HpyCH4V	TGCA	6 cut(s)	1329, 1472, 1534, 1685, 1807, 1864
HpyF10VI	GCNNNNNNNGC	9 cut(s)	95, 104, 286, 301, 347, 364, 373, 470, 644
HpyF3I	CTNAG	9 cut(s)	588, 699, 1020, 1074, 1098, 1251, 1263, 1385, 1625
HpySE526I	ACGT	4 cut(s)	326, 386, 1259, 1448
Hsp92I	GRCGYC	1 cut(s)	512
HspAI	GCGC	1 cut(s)	512
KasI	GGCGCC	1 cut(s)	511
KflI	GGGWCCC	1 cut(s)	400
KroI	GCCGGC	1 cut(s)	412
KroNI	GCCGGC	1 cut(s)	414
Ksp22I	TGATCA	1 cut(s)	1903
KspI	CCGCGG	1 cut(s)	305
Kzo9I	GATC	8 cut(s)	128, 592, 603, 1101, 1477, 1482, 1784, 1903
LmnI	GCTCC	6 cut(s)	162, 306, 320, 613, 1383, 1909
Lsp1109I	GCAGC	5 cut(s)	136, 625, 846, 879, 1013
LweI	GCATC	2 cut(s)	634, 1508
MabI	ACCWGGT	1 cut(s)	1332
MaeI	CTAG	3 cut(s)	555, 1664, 1674
MaeII	ACGT	4 cut(s)	326, 386, 1259, 1448
MaeIII	GTNAC	8 cut(s)	11, 430, 478, 487, 1053, 1349, 1629, 1777
MalI	GATC	8 cut(s)	130, 594, 605, 1103, 1479, 1484, 1786, 1905
MboI	GATC	8 cut(s)	128, 592, 603, 1101, 1477, 1482, 1784, 1903
MflI	RGATCY	3 cut(s)	592, 1482, 1784
MhlI	GDGCHC	5 cut(s)	311, 506, 1257, 1345, 1605
MlsI	TGGCCA	1 cut(s)	654
MluCI	AATT	5 cut(s)	161, 736, 840, 1440, 1933
MluNI	TGGCCA	1 cut(s)	654
Mly113I	GGCGCC	1 cut(s)	512
MlyI	GAGTC	6 cut(s)	268, 781, 1061, 1646, 1841, 1883
MmeI	TCCRAC	2 cut(s)	447, 1029
Mox20I	TGGCCA	1 cut(s)	654
MroNI	GCCGGC	1 cut(s)	412
MroXI	GAANNNNTTC	1 cut(s)	914
MscI	TGGCCA	1 cut(s)	654
MseI	TTAA	9 cut(s)	609, 633, 705, 821, 1233, 1523, 1596, 1920, 1936
MslI	CAYNNNNRTG	2 cut(s)	754, 760
Msp20I	TGGCCA	1 cut(s)	654
MspA1I	CMGCKG	1 cut(s)	304
MspI	CCGG	4 cut(s)	413, 492, 648, 1381
MspR9I	CCNGG	5 cut(s)	493, 580, 687, 920, 1334
Mva1269I	GAATGC	1 cut(s)	589
MvaI	CCWGG	4 cut(s)	580, 687, 920, 1334
MvnI	CGCG	2 cut(s)	304, 464
MwoI	GCNNNNNNNGC	9 cut(s)	95, 104, 286, 301, 347, 364, 373, 470, 644
NaeI	GCCGGC	1 cut(s)	414
NarI	GGCGCC	1 cut(s)	512
NciI	CCSGG	1 cut(s)	493
NcoI	CCATGG	1 cut(s)	727
NdeII	GATC	8 cut(s)	128, 592, 603, 1101, 1477, 1482, 1784, 1903
NgoMIV	GCCGGC	1 cut(s)	412
NheI	GCTAGC	1 cut(s)	554
NlaIV	GGNNCC	8 cut(s)	308, 401, 402, 513, 1316, 1379, 1466, 1484
NmuCI	GTSAC	6 cut(s)	430, 487, 1053, 1349, 1629, 1777
NspI	RCATGY	3 cut(s)	1183, 1538, 1826
NspV	TTCGAA	1 cut(s)	539
PaeI	GCATGC	1 cut(s)	1538
PaeR7I	CTCGAG	4 cut(s)	16, 1292, 1604, 1726
PciI	ACATGT	1 cut(s)	1822
PcsI	WCGNNNNNNNCGW	2 cut(s)	133, 461
PctI	GAATGC	1 cut(s)	589
PdiI	GCCGGC	1 cut(s)	414
PdmI	GAANNNNTTC	1 cut(s)	914
PfeI	GAWTC	6 cut(s)	21, 1267, 1433, 1509, 1586, 1667
PfoI	TCCNGGA	3 cut(s)	578, 685, 918
PleI	GAGTC	6 cut(s)	268, 780, 1060, 1645, 1840, 1882
PluTI	GGCGCC	1 cut(s)	515
PmaCI	CACGTG	2 cut(s)	327, 387
PmlI	CACGTG	2 cut(s)	327, 387
PpsI	GAGTC	6 cut(s)	268, 780, 1060, 1645, 1840, 1882
Ppu21I	YACGTR	2 cut(s)	327, 387
PpuMI	RGGWCCY	1 cut(s)	400
PscI	ACATGT	1 cut(s)	1822
Psp124BI	GAGCTC	1 cut(s)	1605
Psp5II	RGGWCCY	1 cut(s)	400
Psp6I	CCWGG	4 cut(s)	578, 685, 918, 1332
PspCI	CACGTG	2 cut(s)	327, 387
PspEI	GGTNACC	1 cut(s)	430
PspGI	CCWGG	4 cut(s)	578, 685, 918, 1332
PspN4I	GGNNCC	8 cut(s)	308, 401, 402, 513, 1316, 1379, 1466, 1484
PspPI	GGNCC	3 cut(s)	330, 400, 1649
PspPPI	RGGWCCY	1 cut(s)	400
PspXI	VCTCGAGB	1 cut(s)	1292
PstNI	CAGNNNCTG	4 cut(s)	149, 1589, 1700, 1912
PsuI	RGATCY	3 cut(s)	592, 1482, 1784
RsaI	GTAC	4 cut(s)	68, 122, 861, 1130
RsaNI	GTAC	4 cut(s)	67, 121, 860, 1129
RseI	CAYNNNNRTG	2 cut(s)	754, 760
SacI	GAGCTC	1 cut(s)	1605
SacII	CCGCGG	1 cut(s)	305
SaqAI	TTAA	9 cut(s)	609, 633, 705, 821, 1233, 1523, 1596, 1920, 1936
Sau3AI	GATC	8 cut(s)	128, 592, 603, 1101, 1477, 1482, 1784, 1903
Sau96I	GGNCC	3 cut(s)	330, 400, 1649
SchI	GAGTC	6 cut(s)	268, 781, 1061, 1646, 1841, 1883
ScrFI	CCNGG	5 cut(s)	493, 580, 687, 920, 1334
SduI	GDGCHC	5 cut(s)	311, 506, 1257, 1345, 1605
SexAI	ACCWGGT	1 cut(s)	1332
SfaNI	GCATC	2 cut(s)	634, 1508
SfcI	CTRYAG	1 cut(s)	1527
SfoI	GGCGCC	1 cut(s)	513
Sfr274I	CTCGAG	4 cut(s)	16, 1292, 1604, 1726
Sfr303I	CCGCGG	1 cut(s)	305
SfuI	TTCGAA	1 cut(s)	539
SgrBI	CCGCGG	1 cut(s)	305
SinI	GGWCC	2 cut(s)	330, 400
SlaI	CTCGAG	4 cut(s)	16, 1292, 1604, 1726
SmiMI	CAYNNNNRTG	2 cut(s)	754, 760
SmlI	CTYRAG	6 cut(s)	16, 1142, 1292, 1604, 1634, 1726
SmoI	CTYRAG	6 cut(s)	16, 1142, 1292, 1604, 1634, 1726
SphI	GCATGC	1 cut(s)	1538
Sse9I	AATT	5 cut(s)	161, 736, 840, 1440, 1933
SspDI	GGCGCC	1 cut(s)	511
SspMI	CTAG	3 cut(s)	555, 1664, 1674
SstI	GAGCTC	1 cut(s)	1605
StyD4I	CCNGG	5 cut(s)	491, 578, 685, 918, 1332
StyI	CCWWGG	1 cut(s)	727
TaaI	ACNGT	5 cut(s)	487, 508, 548, 883, 1528
TaiI	ACGT	4 cut(s)	329, 389, 1262, 1451
TaqII	GACCGA	2 cut(s)	414, 449
TasI	AATT	5 cut(s)	161, 736, 840, 1440, 1933
TauI	GCSGC	8 cut(s)	256, 259, 358, 370, 373, 376, 500, 517
TfiI	GAWTC	6 cut(s)	21, 1267, 1433, 1509, 1586, 1667
Tru1I	TTAA	9 cut(s)	609, 633, 705, 821, 1233, 1523, 1596, 1920, 1936
Tru9I	TTAA	9 cut(s)	609, 633, 705, 821, 1233, 1523, 1596, 1920, 1936
TscAI	CASTG	1 cut(s)	553
TseFI	GTSAC	6 cut(s)	430, 487, 1053, 1349, 1629, 1777
TseI	GCWGC	5 cut(s)	149, 638, 834, 867, 1001
Tsp45I	GTSAC	6 cut(s)	430, 487, 1053, 1349, 1629, 1777
TspDTI	ATGAA	4 cut(s)	86, 1007, 1289, 1776
TspRI	CASTG	1 cut(s)	553
VpaK11BI	GGWCC	2 cut(s)	330, 400
XapI	RAATTY	1 cut(s)	1440
XceI	RCATGY	3 cut(s)	1183, 1538, 1826
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1036
XhoI	CTCGAG	4 cut(s)	16, 1292, 1604, 1726
XmnI	GAANNNNTTC	1 cut(s)	914
XspI	CTAG	3 cut(s)	555, 1664, 1674

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.