Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005482

Physical Location & Seq

Genomic Coordinates Forward (+)

24735212 .. 24739791

Gene Structure 4580 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1279300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGAGAGAAATCGTCACTCTCCAAGTTGGAAGCTTTGCAAATTTCGTGGGCTCCCATTTCTGGAACTTCCAGGATGAACTACTTGGGTTGGCGGAGGACCCTAACTCCGACCCGGTATTCAAGAATCAGTCTTTGAACATGGACGTGCTCTACCGCTCTGGTGAGACGCACCAGGGCACTGTGACTTACACTCCCCGCCTCGTTTCGGTTGATCTTCAAGGTTCCCTCGGGTCTATGAGTACACGTGGCACGTTGTACGATGAGAGTTCATCTGCATCATCAAACATTTACACATGGGGTGGTAATGTTACTACTCAAGTCGCCGAGCCTCATAAAAAGAATTTGTTCTTACGAAGTTTGTATGAGGAAGAGCCGGTGAATCTTTTGACTCTTGGAAATGGTGTCAATGCTGGGGGGAAGAGTTGTCGAGGAGAAATTCATGACAGTGACAAAGTGGAGAGTTTAGAAAATGGGGTCCAGTACTGGACTGACTTTTCAAAAGTTCACTATCATCCCCAGAGTTTATATGAATTGAGTGGATTGTGGGTAGATCCTCAGAAATTTGACAATTACGGGATTGGAAGAGAGTCCTTATCAGCAGGTTTACAGGGAGAAGAGGTTAGTGAGAGGCTTCGCTTTTTCGTAGAAGAGTGCGACCATATTCAGGGTTTTCAATTCATCGTTGATGACTCAGGAGGTTTCTCTCCTCTAGCTGTGGATGTTCTGGAGAGTATTGCAGATGAATACACAAATGCTCCAGTGTTGCTCTATGCTGTGCGTGGTCCTGGTTCTTCCATGGACCCCAGAAGCCAGAAGCAGAGAGTTAGTAGGAAGCTTCACGATGCAGTTTCATTTTCAAAGCTATCATCCTTATGCAAATTGATTGTTCCAGTCGGGTTACCCTCATTGAGTACAAGTAAAGCTTCAAGATTTCTCAGCATCAATGATAAAAAGCCTTATCACTGCAGTGCAGCTTATGCTGCTACCCTACATTCTATTAGTCTTCCGTTCCGAATGGAACCACTCGGGCCCACTGCAGACTCATCTTATGCTTCTGGTGCTGTGACTATTAGTGAGGTTGTGCAAATCCTAGCAGGGCAAGCTAGGCAGAATATGGTTGCCATTCTGGATGCTGCAATGCCATCCCCTTCTTTAACTGGGAATTGGGTTGGTCAAAATTTAACAGGGAATTTGCAGCCATTGACACCAGAGATAGCAGAAGATGTCGAGGATTTACAGTCATTAGAATCCATGATAGTCCATGGGGCCCTAGGATCAGGAGGTCATCGAGCATCAATTTCTGAAGTTAAGGACACAGTCCATGCTGCCTATGAGCATGGAATAACAAGGCCTATGTTCTGCCATCTGTCAGTGGCTCAATGTCCTCTTCCAATACCTTTGCCTTTTCCTTCAATCTTTGGCAACCTTGTTGGCCAACATGGTGAACTTTTGAGCACGCCAATTGTTGGTTCCCCATCAAGGGGATCACTTGATATCCATTCCATCCCCATGGCAGCTAGGCTACGGTCTAGCAGTGCCGTCTTGCCATTTTTAGAGAATAGATTGGGCAGCCTTCGTAGCTGTGGGATTGCCAGAGGAGCACCTGGGGCGGAGCTAGTTAGAAATTGGGGCTTTGCAAAGGATGAATTGGAAGACATGGGTGAGATGCTGTCTAAAATGATTGCAACACTGGATCTGCGTTCTGAAATGTCCTCTGATTCAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Misat_Tub_SegII Tubulin_3

Protein Analysis

576

Amino Acids

62.36

Weight (kDa)

5.16

Isoelectric Point (pI)

45.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Misat_Tub_SegII	PF10644	2 - 121	1.3e-37	Misato Segment II tubulin-like domain
Tubulin_3	PF14881	157 - 338	6.6e-22	Tubulin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005482)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37190 AT4G37190
fragaria_vesca	FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_1g15480 FvH4_5g33502
malus_domestica	MD02G1166800.v1.1 MD15G1279300.v1.1
prunus_persica	Prupe.7G138000_v2.0.a1 Prupe.7G138000_v2.0.a1 Prupe.7G138000_v2.0.a1
pyrus_communis	pycom15g24210 pycom15g24220
rosa_chinensis	RchiOBHm_Chr2g0104711
rosa_laevigata	RLG00000017353
rosa_multiflora	Rmu_co8514121.1_g000001 Rmu_sc0002316.1_g000019
rosa_roxburghii	Rroxscaffold_2G00138810
rosa_rugosa	Rorug02G0121900
rosa_samantha	Rh2AG172200 Rh2BG179800 Rh2CG177300 Rh2DG178500
rosa_wichuraiana	Rw0G008630 Rw2G013480 Rw2G013540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	590
AccB7I	CCANNNNNTGG	1 cut(s)	1466
AccBSI	CCGCTC	1 cut(s)	156
AciI	CCGC	4 cut(s)	92, 154, 196, 1610
AclWI	GGATC	4 cut(s)	545, 1282, 1492, 1701
AcoI	YGGCCR	1 cut(s)	1432
AcsI	RAATTY	6 cut(s)	40, 340, 435, 560, 1177, 1189
AcuI	CTGAAG	1 cut(s)	1323
AcvI	CACGTG	1 cut(s)	245
AfaI	GTAC	4 cut(s)	241, 257, 482, 913
AfiI	CCNNNNNNNGG	7 cut(s)	60, 205, 664, 1466, 1479, 1480, 1481
AflIII	ACRYGT	1 cut(s)	242
AgsI	TTSAA	8 cut(s)	121, 136, 218, 498, 674, 858, 927, 1413
AjiI	CACGTC	1 cut(s)	145
AjnI	CCWGG	4 cut(s)	69, 171, 784, 1603
AjuI	GAANNNNNNNTTGG	2 cut(s)	1453, 1485
AleI	CACNNNNGTG	1 cut(s)	966
Alw21I	GWGCWC	3 cut(s)	150, 1457, 1603
Alw26I	GTCTC	1 cut(s)	158
AlwI	GGATC	4 cut(s)	545, 1282, 1492, 1701
Ama87I	CYCGRG	2 cut(s)	227, 1025
AoxI	GGCC	4 cut(s)	1028, 1266, 1349, 1432
ApaI	GGGCCC	2 cut(s)	1032, 1270
ApeKI	GCWGC	7 cut(s)	971, 980, 1133, 1195, 1325, 1514, 1569
ApoI	RAATTY	6 cut(s)	40, 340, 435, 560, 1177, 1189
Asp700I	GAANNNNTTC	1 cut(s)	344
AspA2I	CCTAGG	1 cut(s)	1270
AspS9I	GGNCC	8 cut(s)	97, 475, 782, 799, 1028, 1029, 1266, 1267
AsuC2I	CCSGG	1 cut(s)	113
AsuHPI	GGTGA	4 cut(s)	173, 388, 1454, 1673
AvaI	CYCGRG	2 cut(s)	227, 1025
AvaII	GGWCC	4 cut(s)	97, 475, 782, 799
AvrII	CCTAGG	1 cut(s)	1270
BaeGI	GKGCMC	3 cut(s)	179, 1032, 1270
BalI	TGGCCA	1 cut(s)	1434
BanII	GRGCYC	3 cut(s)	53, 1032, 1270
BbrPI	CACGTG	1 cut(s)	245
BbsI	GAAGAC	2 cut(s)	995, 1659
Bbv12I	GWGCWC	3 cut(s)	150, 1457, 1603
BbvI	GCAGC	7 cut(s)	967, 983, 1120, 1207, 1312, 1526, 1581
BccI	CCATC	4 cut(s)	1150, 1371, 1483, 1511
BceAI	ACGGC	1 cut(s)	1523
BciT130I	CCWGG	4 cut(s)	71, 173, 786, 1605
BcnI	CCSGG	1 cut(s)	113
BcoDI	GTCTC	1 cut(s)	158
BfaI	CTAG	7 cut(s)	710, 1091, 1104, 1271, 1518, 1530, 1616
BfmI	CTRYAG	2 cut(s)	964, 1035
BfuAI	ACCTGC	1 cut(s)	590
BisI	GCNGC	7 cut(s)	972, 981, 1134, 1196, 1326, 1515, 1570
BlnI	CCTAGG	1 cut(s)	1270
BlsI	GCNGC	7 cut(s)	973, 982, 1135, 1197, 1327, 1516, 1571
BmcAI	AGTACT	1 cut(s)	482
Bme1390I	CCNGG	5 cut(s)	71, 113, 173, 786, 1605
Bme18I	GGWCC	4 cut(s)	97, 475, 782, 799
BmeT110I	CYCGRG	2 cut(s)	227, 1025
BmgBI	CACGTC	1 cut(s)	145
BmgT120I	GGNCC	8 cut(s)	97, 475, 782, 799, 1028, 1029, 1266, 1267
BmrFI	CCNGG	5 cut(s)	71, 113, 173, 786, 1605
BmrI	ACTGGG	1 cut(s)	1167
BmsI	GCATC	6 cut(s)	284, 832, 948, 1120, 1301, 1656
BmuI	ACTGGG	1 cut(s)	1167
BpiI	GAAGAC	2 cut(s)	995, 1659
BpmI	CTGGAG	2 cut(s)	741, 746
BpuEI	CTTGAG	1 cut(s)	300
BpuMI	CCSGG	1 cut(s)	113
BsaAI	YACGTR	1 cut(s)	245
BsaJI	CCNNGG	7 cut(s)	172, 226, 795, 1261, 1270, 1509, 1604
BsaXI	ACNNNNNCTCC	8 cut(s)	89, 119, 175, 205, 603, 633, 739, 769
Bsc4I	CCNNNNNNNGG	7 cut(s)	60, 205, 664, 1466, 1479, 1480, 1481
Bse118I	RCCGGY	1 cut(s)	373
Bse1I	ACTGG	6 cut(s)	478, 488, 758, 890, 1162, 1695
Bse3DI	GCAATG	1 cut(s)	1143
BseBI	CCWGG	4 cut(s)	71, 173, 786, 1605
BseDI	CCNNGG	7 cut(s)	172, 226, 795, 1261, 1270, 1509, 1604
BseGI	GGATG	8 cut(s)	79, 511, 724, 866, 1135, 1142, 1503, 1648
BseLI	CCNNNNNNNGG	7 cut(s)	60, 205, 664, 1466, 1479, 1480, 1481
BseMI	GCAATG	1 cut(s)	1143
BseMII	CTCAG	3 cut(s)	569, 705, 949
BseNI	ACTGG	6 cut(s)	478, 488, 758, 890, 1162, 1695
BseRI	GAGGAG	3 cut(s)	444, 696, 1611
BseSI	GKGCMC	3 cut(s)	179, 1032, 1270
BseXI	GCAGC	7 cut(s)	967, 983, 1120, 1207, 1312, 1526, 1581
BseYI	CCCAGC	1 cut(s)	410
BsgI	GTGCAG	1 cut(s)	990
BshFI	GGCC	4 cut(s)	1030, 1268, 1351, 1434
BsiHKAI	GWGCWC	3 cut(s)	150, 1457, 1603
BsiHKCI	CYCGRG	2 cut(s)	227, 1025
BsiSI	CCGG	2 cut(s)	113, 374
BslI	CCNNNNNNNGG	7 cut(s)	60, 205, 664, 1466, 1479, 1480, 1481
BsmAI	GTCTC	1 cut(s)	158
BsmBI	CGTCTC	1 cut(s)	158
BsnI	GGCC	4 cut(s)	1030, 1268, 1351, 1434
BsoBI	CYCGRG	2 cut(s)	227, 1025
Bsp120I	GGGCCC	2 cut(s)	1028, 1266
Bsp1286I	GDGCHC	7 cut(s)	53, 150, 179, 1032, 1270, 1457, 1603
Bsp143I	GATC	5 cut(s)	211, 550, 1274, 1484, 1693
Bsp19I	CCATGG	3 cut(s)	795, 1261, 1509
BspACI	CCGC	4 cut(s)	92, 154, 196, 1610
BspANI	GGCC	4 cut(s)	1030, 1268, 1351, 1434
BspCNI	CTCAG	3 cut(s)	568, 704, 948
BspHI	TCATGA	1 cut(s)	439
BspMAI	CTGCAG	2 cut(s)	968, 1039
BspMI	ACCTGC	1 cut(s)	590
BspPI	GGATC	4 cut(s)	545, 1282, 1492, 1701
BspQI	GCTCTTC	1 cut(s)	363
BsrBI	CCGCTC	1 cut(s)	156
BsrDI	GCAATG	1 cut(s)	1143
BsrFI	RCCGGY	1 cut(s)	373
BsrI	ACTGG	6 cut(s)	478, 488, 758, 890, 1162, 1695
BssAI	RCCGGY	1 cut(s)	373
BssECI	CCNNGG	7 cut(s)	172, 226, 795, 1261, 1270, 1509, 1604
BssMI	GATC	5 cut(s)	211, 550, 1274, 1484, 1693
BssT1I	CCWWGG	4 cut(s)	795, 1261, 1270, 1509
Bst2UI	CCWGG	4 cut(s)	71, 173, 786, 1605
Bst4CI	ACNGT	5 cut(s)	181, 446, 1239, 1318, 1527
Bst6I	CTCTTC	6 cut(s)	363, 413, 577, 609, 642, 1392
BstAPI	GCANNNNNTGC	1 cut(s)	977
BstBAI	YACGTR	1 cut(s)	245
BstC8I	GCNNGC	2 cut(s)	1101, 1457
BstDEI	CTNAG	3 cut(s)	555, 691, 935
BstDSI	CCRYGG	3 cut(s)	795, 1261, 1509
BstEII	GGTNACC	1 cut(s)	897
BstF5I	GGATG	8 cut(s)	79, 511, 724, 866, 1135, 1142, 1503, 1648
BstKTI	GATC	5 cut(s)	214, 553, 1277, 1487, 1696
BstMAI	GTCTC	1 cut(s)	158
BstMBI	GATC	5 cut(s)	211, 550, 1274, 1484, 1693
BstMWI	GCNNNNNNNGC	8 cut(s)	977, 980, 1058, 1100, 1105, 1578, 1598, 1607
BstNI	CCWGG	4 cut(s)	71, 173, 786, 1605
BstPI	GGTNACC	1 cut(s)	897
BstSCI	CCNGG	5 cut(s)	69, 111, 171, 784, 1603
BstSFI	CTRYAG	2 cut(s)	964, 1035
BstSLI	GKGCMC	3 cut(s)	179, 1032, 1270
BstV1I	GCAGC	7 cut(s)	967, 983, 1120, 1207, 1312, 1526, 1581
BstV2I	GAAGAC	2 cut(s)	995, 1659
BstX2I	RGATCY	2 cut(s)	550, 1693
BstXI	CCANNNNNNTGG	1 cut(s)	1510
BstYI	RGATCY	2 cut(s)	550, 1693
BsuRI	GGCC	4 cut(s)	1030, 1268, 1351, 1434
BtgI	CCRYGG	3 cut(s)	795, 1261, 1509
BtrI	CACGTC	1 cut(s)	145
BtsCI	GGATG	8 cut(s)	79, 511, 724, 866, 1135, 1142, 1503, 1648
BtsI	GCAGTG	4 cut(s)	961, 973, 1032, 1540
BtsIMutI	CAGTG	9 cut(s)	177, 451, 765, 961, 973, 1032, 1377, 1540, 1688
BveI	ACCTGC	1 cut(s)	590
Cac8I	GCNNGC	2 cut(s)	1101, 1457
CciI	TCATGA	1 cut(s)	439
Cfr10I	RCCGGY	1 cut(s)	373
Cfr13I	GGNCC	8 cut(s)	97, 475, 782, 799, 1028, 1029, 1266, 1267
CseI	GACGC	1 cut(s)	175
Csp6I	GTAC	4 cut(s)	240, 256, 481, 912
CspCI	CAANNNNNGTGG	2 cut(s)	27, 62
CviQI	GTAC	4 cut(s)	240, 256, 481, 912
DdeI	CTNAG	3 cut(s)	555, 691, 935
DpnI	GATC	5 cut(s)	213, 552, 1276, 1486, 1695
DpnII	GATC	5 cut(s)	211, 550, 1274, 1484, 1693
EaeI	YGGCCR	1 cut(s)	1432
Eam1104I	CTCTTC	6 cut(s)	363, 413, 577, 609, 642, 1392
EarI	CTCTTC	6 cut(s)	363, 413, 577, 609, 642, 1392
EciI	GGCGGA	2 cut(s)	107, 1625
Eco130I	CCWWGG	4 cut(s)	795, 1261, 1270, 1509
Eco147I	AGGCCT	1 cut(s)	1351
Eco24I	GRGCYC	3 cut(s)	53, 1032, 1270
Eco32I	GATATC	1 cut(s)	1495
Eco47I	GGWCC	4 cut(s)	97, 475, 782, 799
Eco57I	CTGAAG	1 cut(s)	1323
Eco72I	CACGTG	1 cut(s)	245
Eco88I	CYCGRG	2 cut(s)	227, 1025
Eco91I	GGTNACC	1 cut(s)	897
EcoO109I	RGGNCCY	3 cut(s)	97, 1266, 1267
EcoO65I	GGTNACC	1 cut(s)	897
EcoRII	CCWGG	4 cut(s)	69, 171, 784, 1603
EcoRV	GATATC	1 cut(s)	1495
EcoT14I	CCWWGG	4 cut(s)	795, 1261, 1270, 1509
EcoT38I	GRGCYC	3 cut(s)	53, 1032, 1270
ErhI	CCWWGG	4 cut(s)	795, 1261, 1270, 1509
Esp3I	CGTCTC	1 cut(s)	158
FalI	AAGNNNNNCTT	2 cut(s)	907, 939
FauI	CCCGC	1 cut(s)	203
Fnu4HI	GCNGC	7 cut(s)	972, 981, 1134, 1196, 1326, 1515, 1570
FokI	GGATG	8 cut(s)	86, 498, 731, 853, 1129, 1142, 1490, 1655
FriOI	GRGCYC	3 cut(s)	53, 1032, 1270
Fsp4HI	GCNGC	7 cut(s)	972, 981, 1134, 1196, 1326, 1515, 1570
FspBI	CTAG	7 cut(s)	710, 1091, 1104, 1271, 1518, 1530, 1616
GluI	GCNGC	7 cut(s)	972, 981, 1134, 1196, 1326, 1515, 1570
GsaI	CCCAGC	1 cut(s)	414
GsuI	CTGGAG	2 cut(s)	741, 746
HaeIII	GGCC	4 cut(s)	1030, 1268, 1351, 1434
HapII	CCGG	2 cut(s)	113, 374
HgaI	GACGC	1 cut(s)	175
HindIII	AAGCTT	3 cut(s)	31, 833, 921
HinfI	GANTC	8 cut(s)	124, 379, 388, 587, 689, 1040, 1247, 1718
HpaII	CCGG	2 cut(s)	113, 374
HphI	GGTGA	4 cut(s)	173, 388, 1454, 1673
Hpy166II	GTNNAC	4 cut(s)	242, 505, 605, 1445
Hpy188I	TCNGA	7 cut(s)	109, 558, 1013, 1303, 1705, 1717, 1723
Hpy188III	TCNNGA	9 cut(s)	61, 121, 440, 693, 725, 839, 927, 1127, 1278
Hpy8I	GTNNAC	4 cut(s)	242, 505, 605, 1445
HpyAV	CCTTC	3 cut(s)	1158, 1419, 1583
HpyCH4III	ACNGT	5 cut(s)	181, 446, 1239, 1318, 1527
HpyCH4IV	ACGT	3 cut(s)	144, 244, 251
HpyF10VI	GCNNNNNNNGC	8 cut(s)	977, 980, 1058, 1100, 1105, 1578, 1598, 1607
HpyF3I	CTNAG	3 cut(s)	555, 691, 935
HpySE526I	ACGT	3 cut(s)	144, 244, 251
Kzo9I	GATC	5 cut(s)	211, 550, 1274, 1484, 1693
LguI	GCTCTTC	1 cut(s)	363
LmnI	GCTCC	4 cut(s)	56, 760, 1598, 1612
Lsp1109I	GCAGC	7 cut(s)	967, 983, 1120, 1207, 1312, 1526, 1581
LweI	GCATC	6 cut(s)	284, 832, 948, 1120, 1301, 1656
MaeI	CTAG	7 cut(s)	710, 1091, 1104, 1271, 1518, 1530, 1616
MaeII	ACGT	3 cut(s)	144, 244, 251
MaeIII	GTNAC	6 cut(s)	13, 181, 307, 446, 897, 1063
MalI	GATC	5 cut(s)	213, 552, 1276, 1486, 1695
MbiI	CCGCTC	1 cut(s)	156
MboI	GATC	5 cut(s)	211, 550, 1274, 1484, 1693
MfeI	CAATTG	1 cut(s)	1461
MflI	RGATCY	2 cut(s)	550, 1693
MhlI	GDGCHC	7 cut(s)	53, 150, 179, 1032, 1270, 1457, 1603
MlsI	TGGCCA	1 cut(s)	1434
MluNI	TGGCCA	1 cut(s)	1434
MlyI	GAGTC	4 cut(s)	382, 596, 683, 1034
MmeI	TCCRAC	2 cut(s)	7, 132
Mox20I	TGGCCA	1 cut(s)	1434
MroXI	GAANNNNTTC	1 cut(s)	344
MscI	TGGCCA	1 cut(s)	1434
MseI	TTAA	4 cut(s)	1154, 1181, 1308, 1726
MslI	CAYNNNNRTG	5 cut(s)	143, 444, 871, 966, 1508
Msp20I	TGGCCA	1 cut(s)	1434
MspI	CCGG	2 cut(s)	113, 374
MspR9I	CCNGG	5 cut(s)	71, 113, 173, 786, 1605
MunI	CAATTG	1 cut(s)	1461
MvaI	CCWGG	4 cut(s)	71, 173, 786, 1605
MwoI	GCNNNNNNNGC	8 cut(s)	977, 980, 1058, 1100, 1105, 1578, 1598, 1607
NciI	CCSGG	1 cut(s)	113
NcoI	CCATGG	3 cut(s)	795, 1261, 1509
NdeII	GATC	5 cut(s)	211, 550, 1274, 1484, 1693
NmeAIII	GCCGAG	1 cut(s)	349
NmuCI	GTSAC	4 cut(s)	13, 181, 446, 1063
OliI	CACNNNNGTG	1 cut(s)	966
PagI	TCATGA	1 cut(s)	439
PceI	AGGCCT	1 cut(s)	1351
PciSI	GCTCTTC	1 cut(s)	363
PdmI	GAANNNNTTC	1 cut(s)	344
PfeI	GAWTC	4 cut(s)	124, 379, 1247, 1718
PflFI	GACNNNGTC	1 cut(s)	1316
PflMI	CCANNNNNTGG	1 cut(s)	1466
PfoI	TCCNGGA	1 cut(s)	69
PkrI	GCNGC	7 cut(s)	973, 982, 1135, 1197, 1327, 1516, 1571
PleI	GAGTC	4 cut(s)	382, 595, 683, 1034
PmaCI	CACGTG	1 cut(s)	245
PmlI	CACGTG	1 cut(s)	245
PpsI	GAGTC	4 cut(s)	382, 595, 683, 1034
Ppu21I	YACGTR	1 cut(s)	245
PpuMI	RGGWCCY	1 cut(s)	97
Psp5II	RGGWCCY	1 cut(s)	97
Psp6I	CCWGG	4 cut(s)	69, 171, 784, 1603
PspCI	CACGTG	1 cut(s)	245
PspEI	GGTNACC	1 cut(s)	897
PspFI	CCCAGC	1 cut(s)	410
PspGI	CCWGG	4 cut(s)	69, 171, 784, 1603
PspOMI	GGGCCC	2 cut(s)	1028, 1266
PspPI	GGNCC	8 cut(s)	97, 475, 782, 799, 1028, 1029, 1266, 1267
PspPPI	RGGWCCY	1 cut(s)	97
PstI	CTGCAG	2 cut(s)	968, 1039
PsuI	RGATCY	2 cut(s)	550, 1693
PsyI	GACNNNGTC	1 cut(s)	1316
RsaI	GTAC	4 cut(s)	241, 257, 482, 913
RsaNI	GTAC	4 cut(s)	240, 256, 481, 912
RseI	CAYNNNNRTG	5 cut(s)	143, 444, 871, 966, 1508
SapI	GCTCTTC	1 cut(s)	363
SaqAI	TTAA	4 cut(s)	1154, 1181, 1308, 1726
SatI	GCNGC	7 cut(s)	972, 981, 1134, 1196, 1326, 1515, 1570
Sau3AI	GATC	5 cut(s)	211, 550, 1274, 1484, 1693
Sau96I	GGNCC	8 cut(s)	97, 475, 782, 799, 1028, 1029, 1266, 1267
ScaI	AGTACT	1 cut(s)	482
SchI	GAGTC	4 cut(s)	382, 596, 683, 1034
ScrFI	CCNGG	5 cut(s)	71, 113, 173, 786, 1605
SduI	GDGCHC	7 cut(s)	53, 150, 179, 1032, 1270, 1457, 1603
SfaNI	GCATC	6 cut(s)	284, 832, 948, 1120, 1301, 1656
SfcI	CTRYAG	2 cut(s)	964, 1035
SinI	GGWCC	4 cut(s)	97, 475, 782, 799
SmiMI	CAYNNNNRTG	5 cut(s)	143, 444, 871, 966, 1508
SmlI	CTYRAG	1 cut(s)	315
SmoI	CTYRAG	1 cut(s)	315
SseBI	AGGCCT	1 cut(s)	1351
SsiI	CCGC	4 cut(s)	92, 154, 196, 1610
SspMI	CTAG	7 cut(s)	710, 1091, 1104, 1271, 1518, 1530, 1616
StuI	AGGCCT	1 cut(s)	1351
StyD4I	CCNGG	5 cut(s)	69, 111, 171, 784, 1603
StyI	CCWWGG	4 cut(s)	795, 1261, 1270, 1509
TaaI	ACNGT	5 cut(s)	181, 446, 1239, 1318, 1527
TaiI	ACGT	3 cut(s)	147, 247, 254
TaqI	TCGA	3 cut(s)	427, 1227, 1288
TatI	WGTACW	3 cut(s)	239, 480, 911
TfiI	GAWTC	4 cut(s)	124, 379, 1247, 1718
Tru1I	TTAA	4 cut(s)	1154, 1181, 1308, 1726
Tru9I	TTAA	4 cut(s)	1154, 1181, 1308, 1726
TscAI	CASTG	9 cut(s)	184, 451, 765, 968, 973, 1039, 1377, 1540, 1695
TseFI	GTSAC	4 cut(s)	13, 181, 446, 1063
TseI	GCWGC	7 cut(s)	971, 980, 1133, 1195, 1325, 1514, 1569
Tsp45I	GTSAC	4 cut(s)	13, 181, 446, 1063
TspDTI	ATGAA	8 cut(s)	90, 258, 428, 543, 667, 756, 840, 1659
TspGWI	ACGGA	1 cut(s)	996
TspRI	CASTG	9 cut(s)	184, 451, 765, 968, 973, 1039, 1377, 1540, 1695
Tth111I	GACNNNGTC	1 cut(s)	1316
Van91I	CCANNNNNTGG	1 cut(s)	1466
VpaK11BI	GGWCC	4 cut(s)	97, 475, 782, 799
XapI	RAATTY	6 cut(s)	40, 340, 435, 560, 1177, 1189
XmaJI	CCTAGG	1 cut(s)	1270
XmnI	GAANNNNTTC	1 cut(s)	344
XspI	CTAG	7 cut(s)	710, 1091, 1104, 1271, 1518, 1530, 1616
ZrmI	AGTACT	1 cut(s)	482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.