pycom15g24210

Protein misato homolog 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005482
Physical Location & Seq
Forward (+)
18389938 .. 18391477
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g24210.1

Sequence Viewer

Length: 648 bp
ATGTTAATTTTCTCATATTTCCAGGTTGCCTCCAAATTCCTTCCCTCTCTCCGACCGCTTCTCAGCTCTAGTTCCGGAACCATGAGAGAACTCGTCACTCTCCAAGTTGGAAGCTTTGCAAATTTCGTGGGCTCCCACTTCTGGAACTTCCAGGATGAACTACTTGGGTTGGCGGAGGACCCTTACTCCGACCCGGTATTCAAGAATCAGTCTTTGAACATGGACGTGCTCTACCGCTCTGGTGAGACGCACCAGGGCACTGTGACTTACACTCCCCGCCTCGTTTCGGTTGATCTTCAAGGTTCCCTCGGGTCTATGAGTACACGTGGCACGTTGTATGATGAGAGTTCATCTGCATCATCAAACATTTACACATGGGGTGGTAATGTTACTACTCAAGTCGCCGAGCCTCATAAGAAGAATTTGTTCTTACAAAGTTTGTATGAGGAAGAGCAGGTGAATCTTTTGACTCTTGGAAATGGTGTCAATGCTGGGGAGAAGAGTTGTCGAAGAGAAATTCATGACAGTGACAAAGTGGAGAGTTTAGAAAATGGGGTCCAGTACTGGACTGACTTTTCAAAAGTTCACTATCATCCCCAGAGTTTATATGAATTGAGTGGATTGTGGGTGGATCGCTCAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Misat_Tub_SegII Tubulin_3

Protein Analysis

216

Amino Acids

24.15

Weight (kDa)

4.97

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Misat_Tub_SegII PF10644 29 - 148 3e-37 Misato Segment II tubulin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 445
Acc36I ACCTGC 1 cut(s) 445
AccBSI CCGCTC 1 cut(s) 237
AccIII TCCGGA 1 cut(s) 74
AciI CCGC 4 cut(s) 56, 173, 235, 277
AclWI GGATC 1 cut(s) 639
AcsI RAATTY 5 cut(s) 35, 121, 421, 516, 642
AcvI CACGTG 1 cut(s) 326
AfaI GTAC 2 cut(s) 322, 563
AfiI CCNNNNNNNGG 2 cut(s) 141, 286
AflIII ACRYGT 1 cut(s) 323
AgsI TTSAA 4 cut(s) 202, 217, 299, 579
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 3 cut(s) 21, 150, 252
AluBI AGCT 2 cut(s) 66, 114
AluI AGCT 2 cut(s) 66, 114
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 639
Ama87I CYCGRG 1 cut(s) 308
Aor13HI TCCGGA 1 cut(s) 74
ApoI RAATTY 5 cut(s) 35, 121, 421, 516, 642
Asp700I GAANNNNTTC 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 178, 556
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 254, 469
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 2 cut(s) 178, 556
BaeGI GKGCMC 1 cut(s) 260
BanII GRGCYC 1 cut(s) 134
BbrPI CACGTG 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 231
BciT130I CCWGG 3 cut(s) 23, 152, 254
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 445
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 4 cut(s) 23, 152, 194, 254
Bme18I GGWCC 2 cut(s) 178, 556
BmeT110I CYCGRG 1 cut(s) 308
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 178, 556
BmiI GGNNCC 5 cut(s) 79, 133, 180, 304, 557
BmrFI CCNGG 4 cut(s) 23, 152, 194, 254
BmsI GCATC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 381
BpuMI CCSGG 1 cut(s) 194
BsaAI YACGTR 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 253, 307
BsaWI WCCGGW 1 cut(s) 74
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 256, 286
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 286
Bse1I ACTGG 2 cut(s) 559, 569
BseAI TCCGGA 1 cut(s) 74
BseBI CCWGG 3 cut(s) 23, 152, 254
BseDI CCNNGG 2 cut(s) 253, 307
BseGI GGATG 2 cut(s) 160, 592
BseLI CCNNNNNNNGG 2 cut(s) 141, 286
BseMII CTCAG 1 cut(s) 76
BseNI ACTGG 2 cut(s) 559, 569
BseSI GKGCMC 1 cut(s) 260
BseYI CCCAGC 1 cut(s) 491
Bsh1285I CGRYCG 1 cut(s) 56
BsiEI CGRYCG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 2 cut(s) 75, 194
BslI CCNNNNNNNGG 2 cut(s) 141, 286
BsmAI GTCTC 1 cut(s) 239
BsmBI CGTCTC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 3 cut(s) 134, 231, 260
Bsp13I TCCGGA 1 cut(s) 74
Bsp143I GATC 2 cut(s) 292, 631
BspACI CCGC 4 cut(s) 56, 173, 235, 277
BspCNI CTCAG 1 cut(s) 75
BspEI TCCGGA 1 cut(s) 74
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 5 cut(s) 79, 133, 180, 304, 557
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 1 cut(s) 639
BspQI GCTCTTC 1 cut(s) 444
BsrBI CCGCTC 1 cut(s) 237
BsrI ACTGG 2 cut(s) 559, 569
BssECI CCNNGG 2 cut(s) 253, 307
BssMI GATC 2 cut(s) 292, 631
Bst2UI CCWGG 3 cut(s) 23, 152, 254
Bst4CI ACNGT 2 cut(s) 262, 527
Bst6I CTCTTC 3 cut(s) 444, 494, 505
BstBAI YACGTR 1 cut(s) 326
BstDEI CTNAG 2 cut(s) 62, 637
BstF5I GGATG 2 cut(s) 160, 592
BstKTI GATC 2 cut(s) 295, 634
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 2 cut(s) 292, 631
BstMCI CGRYCG 1 cut(s) 56
BstNI CCWGG 3 cut(s) 23, 152, 254
BstSCI CCNGG 4 cut(s) 21, 150, 192, 252
BstSLI GKGCMC 1 cut(s) 260
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 2 cut(s) 160, 592
BtsIMutI CAGTG 2 cut(s) 258, 532
BveI ACCTGC 1 cut(s) 445
CciI TCATGA 1 cut(s) 520
Cfr13I GGNCC 2 cut(s) 178, 556
CseI GACGC 1 cut(s) 256
Csp6I GTAC 2 cut(s) 321, 562
CspCI CAANNNNNGTGG 2 cut(s) 108, 143
CviAII CATG 4 cut(s) 82, 220, 375, 521
CviJI RGCY 4 cut(s) 66, 114, 132, 409
CviKI_1 RGCY 4 cut(s) 66, 114, 132, 409
CviQI GTAC 2 cut(s) 321, 562
DdeI CTNAG 2 cut(s) 62, 637
DpnI GATC 2 cut(s) 294, 633
DpnII GATC 2 cut(s) 292, 631
Eam1104I CTCTTC 3 cut(s) 444, 494, 505
EarI CTCTTC 3 cut(s) 444, 494, 505
EciI GGCGGA 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 134
Eco47I GGWCC 2 cut(s) 178, 556
Eco72I CACGTG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 178
EcoRII CCWGG 3 cut(s) 21, 150, 252
EcoT38I GRGCYC 1 cut(s) 134
Esp3I CGTCTC 1 cut(s) 239
FaeI CATG 4 cut(s) 85, 223, 378, 524
FatI CATG 4 cut(s) 81, 219, 374, 520
FauI CCCGC 1 cut(s) 284
FokI GGATG 2 cut(s) 167, 579
FriOI GRGCYC 1 cut(s) 134
FspBI CTAG 1 cut(s) 69
GsaI CCCAGC 1 cut(s) 495
HapII CCGG 2 cut(s) 75, 194
HgaI GACGC 1 cut(s) 256
Hin1II CATG 4 cut(s) 85, 223, 378, 524
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 3 cut(s) 205, 460, 469
HpaII CCGG 2 cut(s) 75, 194
HphI GGTGA 2 cut(s) 254, 469
Hpy166II GTNNAC 2 cut(s) 323, 586
Hpy188I TCNGA 3 cut(s) 53, 190, 640
Hpy188III TCNNGA 4 cut(s) 75, 142, 202, 521
Hpy8I GTNNAC 2 cut(s) 323, 586
HpyAV CCTTC 1 cut(s) 50
HpyCH4III ACNGT 2 cut(s) 262, 527
HpyCH4IV ACGT 3 cut(s) 225, 325, 332
HpyCH4V TGCA 2 cut(s) 119, 356
HpyF3I CTNAG 2 cut(s) 62, 637
HpySE526I ACGT 3 cut(s) 225, 325, 332
Hsp92II CATG 4 cut(s) 85, 223, 378, 524
Kpn2I TCCGGA 1 cut(s) 74
Kzo9I GATC 2 cut(s) 292, 631
LguI GCTCTTC 1 cut(s) 444
LmnI GCTCC 1 cut(s) 137
LweI GCATC 1 cut(s) 365
MaeI CTAG 1 cut(s) 69
MaeII ACGT 3 cut(s) 225, 325, 332
MaeIII GTNAC 4 cut(s) 94, 262, 388, 527
MalI GATC 2 cut(s) 294, 633
MbiI CCGCTC 1 cut(s) 237
MboI GATC 2 cut(s) 292, 631
MboII GAAGA 5 cut(s) 287, 430, 461, 511, 522
MhlI GDGCHC 3 cut(s) 134, 231, 260
MluCI AATT 7 cut(s) 6, 35, 121, 421, 516, 611, 642
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 3 cut(s) 76, 88, 213
MnlI CCTC 7 cut(s) 40, 55, 169, 290, 317, 420, 439
MroI TCCGGA 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 224, 525
MspI CCGG 2 cut(s) 75, 194
MspR9I CCNGG 4 cut(s) 23, 152, 194, 254
MvaI CCWGG 3 cut(s) 23, 152, 254
NciI CCSGG 1 cut(s) 194
NdeII GATC 2 cut(s) 292, 631
NlaIII CATG 4 cut(s) 85, 223, 378, 524
NlaIV GGNNCC 5 cut(s) 79, 133, 180, 304, 557
NmeAIII GCCGAG 1 cut(s) 430
NmuCI GTSAC 3 cut(s) 94, 262, 527
PagI TCATGA 1 cut(s) 520
PaqCI CACCTGC 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 2 cut(s) 205, 460
PfoI TCCNGGA 1 cut(s) 150
PleI GAGTC 1 cut(s) 463
PmaCI CACGTG 1 cut(s) 326
PmlI CACGTG 1 cut(s) 326
PpsI GAGTC 1 cut(s) 463
Ppu21I YACGTR 1 cut(s) 326
PpuMI RGGWCCY 1 cut(s) 178
Psp5II RGGWCCY 1 cut(s) 178
Psp6I CCWGG 3 cut(s) 21, 150, 252
PspCI CACGTG 1 cut(s) 326
PspFI CCCAGC 1 cut(s) 491
PspGI CCWGG 3 cut(s) 21, 150, 252
PspN4I GGNNCC 5 cut(s) 79, 133, 180, 304, 557
PspPI GGNCC 2 cut(s) 178, 556
PspPPI RGGWCCY 1 cut(s) 178
RsaI GTAC 2 cut(s) 322, 563
RsaNI GTAC 2 cut(s) 321, 562
RseI CAYNNNNRTG 2 cut(s) 224, 525
SapI GCTCTTC 1 cut(s) 444
SaqAI TTAA 1 cut(s) 5
Sau3AI GATC 2 cut(s) 292, 631
Sau96I GGNCC 2 cut(s) 178, 556
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 4 cut(s) 23, 152, 194, 254
SduI GDGCHC 3 cut(s) 134, 231, 260
SetI ASST 8 cut(s) 27, 68, 116, 228, 304, 328, 335, 459
SfaNI GCATC 1 cut(s) 365
SinI GGWCC 2 cut(s) 178, 556
SmiMI CAYNNNNRTG 2 cut(s) 224, 525
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 7 cut(s) 6, 35, 121, 421, 516, 611, 642
SsiI CCGC 4 cut(s) 56, 173, 235, 277
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 4 cut(s) 21, 150, 192, 252
TaaI ACNGT 2 cut(s) 262, 527
TaiI ACGT 3 cut(s) 228, 328, 335
TaqI TCGA 1 cut(s) 508
TasI AATT 7 cut(s) 6, 35, 121, 421, 516, 611, 642
TatI WGTACW 2 cut(s) 320, 561
TfiI GAWTC 2 cut(s) 205, 460
Tru1I TTAA 1 cut(s) 5
Tru9I TTAA 1 cut(s) 5
TscAI CASTG 2 cut(s) 265, 532
TseFI GTSAC 3 cut(s) 94, 262, 527
Tsp45I GTSAC 3 cut(s) 94, 262, 527
TspDTI ATGAA 4 cut(s) 171, 339, 509, 624
TspRI CASTG 2 cut(s) 265, 532
VpaK11BI GGWCC 2 cut(s) 178, 556
XapI RAATTY 5 cut(s) 35, 121, 421, 516, 642
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.