FvH4_5g33710

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006768
Physical Location & Seq
Reverse (-)
24409041 .. 24423500
14460 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33710.t2

Sequence Viewer

Length: 4080 bp
ATGTCCTCTTCTCTCTTTGCCCCACCCAGAAAACCCCTCTTCAAAACCCTAACACCCAATTCCACCGCCTTCCACACCATGGCCGGAGGAGCAGGCGGCGGAGCTCTCAACACCCAAGCGCTCTCTCTCCTCGCCGCCGTCAACAACCACGGCGATTTGGCTGTCAAGCTCTCCTCTTTAAAACAGGCCAAGGATTTGCTCCTCTCCATCGACTCCTCCTCCGCCGCCGACCTTTTCCCCTACTTGGTCGAGCTCCAGTCCTCCCCCGAAACCCTCGTCCGCTTATCTCTCATCCAGGTGATCGAGGAGATTGGCTTGAGAGCGATGGAGGAGTCATCTGTACTGATGTCTGTCCTATTGGCGTTTTTGAGAGACAGTGATTCTCTTGTCGCAAGGCAATCTATAGTTAGTGGCACGAATGTTTTTGTCGGTGTCTTGGAGGAGTTGACGTTGCAGTTTCACCGGAGGGGTAAAATTGAGATATGGCTTGAAGAACTTTGGTCGTGGATGGTTAAGTTCAAGGATGCTGTATTCACCATTGCAGTACAGCCTGGTTCTGCTGGAACAAAGTTGCTCGCGTTGAAATTTTTGGAAACATATGTTTTGCTATTCACATCAGACACCGACGATTCTGAAAAACCTATCGCAGAAGGATGTAGAAGAGGTTTCAATATTTCGTGGCTGGTTGGGAGACACCCTATTCTCGACTCGTACATGCTCATGTCAGAAGCAAATAGGGCTCTTGGAATTCTATTGAATTTGTTGCAGTCAGCTGGTAGTCTTCGGGGTTCCTTGACAATTGCCATTGTTAATTGTCTTGCAGCTGTAGCAAGAAAGAGGCCAGTTCACTATGGCACCGTTGTTTCTGCACTATTTGATTTTGATCCAAACTTTGAAGCAGCAAAAGGACGCCATGTTCCCAGTATCCAGTATTCTTTAAGAACTGCCTTTTTGGGATTCCTTCGGTGCACTAGTCCAGTGATTGTAGAGTCAAGAGATAGATTGCTTAGGGCTCTACGCTCTATGAATGCTGGGGATGCTGCTGATCAAGTAATCCGCCAAGTTGATAAAATGCTAAAATACAATGAGCGGGCTTCACGTGATGCTCGGTCTGGAAAGGATGATCACCAGTCAAGCCAACTGCCTGTTTCAGGGGACCTGGTAAGAAAAAGACCTTCACCTCTTGATGTTGAAGAGTCAGCTAATGGTCATGAGATACCTTCCAAGCGAAGTCGTTATACTCATGAAGCCTATTCAACATTGCCAGTTCAAAAGAATGATTCTGGGTGGGATACAACTTCTGTAAATGGAGTGTCCTCTGAGCTTCCTATGCTAGATGGTGAGGTGACTCCTGTGGAGCAAATGATTACTGTAATTGGTGCATTGCTTGCTGAAGGAGAAAGAGGTGCTGAATCACTTGAAATTCTTGTTTCGACAATCCATCCTGATCTGTTGGCCGATATTGTAATAACGAATATGAGGCACTTGCCGAAGATGCCGCCACCCTTAGCAAGGCCAGGGCTGCCAGTTGCTCGGCAGATAGGTTCTTTAAGTAGTTCGGCACAAGTTATCTCAGAGTCTCCAACATCATCTGTGCAGTCTCCAGTTTTGGCTGCCCAAATGTCTTTTTCTTCAGCAACTGTTAACAGCTTATCAGTTGCTGATACTTCCAATGTGAATAATCTTCCCGCTGATTCCAAGCGTGACCCACGAAGGGATCCTCGTCGCCTTGATCCACGGAGTATAGCAGTTTCTGCTGAACTGGCATCCTCTCCCGCTGTAGAGGATACTACTTCTATGCAATCTGACATCGATGGTTCAGTTTCCTTAAATAAGCTTAATTCACATCCGATTTTGACCACTGTTGAATCTACTTTAGTAACTCCCAATCCCAAAACAGAAAGTGATGGTATGATTTTGGATGGTCAATTGGTTTCTGGAACTGACCAACCAACTCCCATGGATGAGATTCTGGATGGACCTGTCGAAGATGACCCTACTTTGAAGGTGAATGTATCTTCAGATCTAACAGATTCTCGTGTGCAAACTGATGAGGACCTTGAAGCAATGCCATTATCAGATGTTGGATTAGCAGATGATGATTATACCACATCTTTTATAGAATCTGATCAGCGTTCTCCTGCCCTTTCAAACACATCTGAAGAGATATGTCAGGACCTGCCTGATGTTCCCATATATATTGAGTTGACACAAGAGCAGAAACAAAGACTAGGGCATATGGCTGTTGAGCGAATTATTCAATCATACAAGCATTTGCACGGAACAGATTATAGTCAGATGCGCTTGGCATTACTAGCTCGATTGGTTGCTCAGATTGATGTGGATGATGAAATTATTGTGATGCTGCATAAACATATAGTTGTGGATTACCAACAGAAAAAGGGGCATGAGCTAGTATTGCATATCTTGTACCATCTGGAAGCTCTGGCTCTGTCCGAATCAGTTGAAAGCTCCACATTTGCTGTTATGTATGAAAAGTTCCTCCTGGCAGTGGCTAAGTGTTTGCTGGAGTCCTTTCCAGCTTCAGACAAGTCATTTAGTAGACTTCTTGGCGAAGTTCCAGTCCTGCCCAACTCCACTTTGAAACTACTAGATGATCTCTGCTATTCTGATGTTATTGATCAACATGGAAAAGATGTTAGGGACACTGAACGTGTCACTCAGGGACTTGGTGCTGTCTGGAGTTTAATTCTAGGGCGGCCACAATATCGACAAAGCTGCTTAGACATCACTTTGAAGTGTGCTGTTCATCCACAAGATGACATCCGCACCAGAGGTGTCCGACTGGTGGCAAACAAACTCTATCAGTTGAGTTACATATCAGAAGTTATTGAAAAATTTGCGACAGATATGTTGTTGTCTGCTGTTGAACAGCCTACATCAGGTATAGAGCATTCACAATCGGAGTCCACAGGGCGAAAAACTGATGGAACGTTGGGAAGCCAAGAAACATCAGTTAATCATGTGCAAAATTTGGAGTTCGCAAATTCTGAAAATGATTCCATAACGAAGGAACGACCAGTGTCAATGATGTCAATTCCTGAAGTTCAGCGACTCATTTCTTTGTTTTTTGCTTTATGTACAAAGAAACCTAGTCTTATTCAACTTGTATTTAATACTTACGGGTGTGCCCCACAAGCTGTAAAGCAGGCGTTTGATCGCAATATTCCTGTTCTTATAAGGGCCCTAGGGTCGTCAAATACAGATTTGCTTCACATCATATCTGATCCTCCGCAAGGAAGTGAAAACCTTTTGATGTTGGTGCTGCAACAATTGACTCAAGAGAGAACACCATCGTCTGATCTGATAGGCACTGTTAAACATTTGTATGAAACTAAATTAAAGGATGTCACAATTCTCATTCCAATGTTGTCTTCACTTACTAAAAATGAGGTTTTACCTATATTCCCTCGGCTTGTTGCCCTTCCATTAGAAAAATTCCAGACTGCACTTGCTCACATACTCCAGGGTTCAGCTCATACAGGTCCAGCTTTGACTCCTGCAGAAGTGTTGGTTTCCATCCACAATATTGTTCCCGACAAAGAAGGCCTCACACTTAAGAAGATAACAGATGTTTGCTCAGCTTGTTTTGAGCAGCGCACAGTTTTCACGCAGCAGGTCTTGGCAAAGGCTTTGAATCAGATGGTTGATCAAACTCCCATTCCTCTTCTCTTTATGAGAACAGTTATTCAGGCAATTGATGCCTTTCCTTCATTGGTTGATTTTGTTATGGAAATACTTTCTAAACTCGTAAGGAAACAGGTTTGGAGAATGCCAAAGCTTTGGGTAGGGTTCTTGAAATGTGCTTCTCAAACCCAGCCACATTCTTTCCATGTATTGTTGCAGTTGCCACCCCCACAACTCGAAAGTGCTTTGAACAAGTATGCGAATGTCAAAGGTCCCCTCGCTGCATATGCTAGCCAGGCTAGTATAAAAGCTTCGCTCTCTAGACCAACACTTGCAGTTCTTGGTCTTGCAAATGAACCGCATTTGCACCAATCGCATTTGTCATCACCACCGTTTCACCCTACAGATGCAACTTCATCAACTCCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04530 map03015 map04530
Pfam Domains
Protein Families
DUF3453 Symplekin_C

Protein Analysis

1360

Amino Acids

148.9

Weight (kDa)

5.56

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYMPK_PTA1_N PF11935 128 - 343 8.8e-37 Symplekin/PTA1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3200
Acc36I ACCTGC 2 cut(s) 2187, 3628
AccB1I GGYRCC 1 cut(s) 854
AccB7I CCANNNNNTGG 1 cut(s) 79
AccBSI CCGCTC 1 cut(s) 1090
AccI GTMKAC 1 cut(s) 2563
AccII CGCG 1 cut(s) 578
AclI AACGTT 1 cut(s) 2954
AclWI GGATC 5 cut(s) 878, 1712, 1725, 1727, 3242
AcoI YGGCCR 3 cut(s) 81, 1455, 2720
AcsI RAATTY 8 cut(s) 584, 747, 757, 1422, 2858, 2992, 3007, 3458
AcuI CTGAAG 6 cut(s) 1413, 1617, 2004, 2181, 2529, 3084
AcvI CACGTG 1 cut(s) 1100
AcyI GRCGYC 1 cut(s) 910
AfaI GTAC 5 cut(s) 342, 546, 713, 2432, 3103
AfeI AGCGCT 1 cut(s) 120
AfiI CCNNNNNNNGG 9 cut(s) 79, 244, 1151, 1512, 1714, 2512, 2809, 2903, 3257
AflII CTTAAG 1 cut(s) 3578
AflIII ACRYGT 1 cut(s) 2674
AhlI ACTAGT 1 cut(s) 971
AjnI CCWGG 7 cut(s) 294, 550, 1158, 1516, 2505, 3486, 3942
AjuI GAANNNNNNNTTGG 2 cut(s) 3379, 3411
AloI GAACNNNNNNTCC 2 cut(s) 900, 932
Alw21I GWGCWC 3 cut(s) 106, 255, 971
Alw26I GTCTC 4 cut(s) 366, 685, 1584, 1605
Alw44I GTGCAC 1 cut(s) 967
AlwI GGATC 5 cut(s) 878, 1712, 1725, 1727, 3242
AlwNI CAGNNNCTG 4 cut(s) 1640, 1661, 1754, 2179
Aor51HI AGCGCT 1 cut(s) 120
AoxI GGCC 8 cut(s) 81, 186, 839, 1455, 1514, 2720, 3204, 3568
ApaI GGGCCC 1 cut(s) 3208
ApaLI GTGCAC 1 cut(s) 967
ApoI RAATTY 8 cut(s) 584, 747, 757, 1422, 2858, 2992, 3007, 3458
ArsI GACNNNNNNTTYG 4 cut(s) 261, 293, 2568, 2600
AspA2I CCTAGG 1 cut(s) 3208
AspLEI GCGC 3 cut(s) 121, 2304, 3621
AspS9I GGNCC 8 cut(s) 1156, 1979, 2056, 2176, 3204, 3205, 3506, 3920
AsuNHI GCTAGC 1 cut(s) 3938
AvaII GGWCC 6 cut(s) 1156, 1979, 2056, 2176, 3506, 3920
AvrII CCTAGG 1 cut(s) 3208
BaeGI GKGCMC 3 cut(s) 971, 3154, 3208
BamHI GGATCC 1 cut(s) 1717
BanI GGYRCC 1 cut(s) 854
BanII GRGCYC 5 cut(s) 106, 255, 742, 1015, 3208
BarI GAAGNNNNNNTAC 4 cut(s) 1222, 1254, 3943, 3975
BauI CACGAG 1 cut(s) 2037
BbrPI CACGTG 1 cut(s) 1100
BbsI GAAGAC 2 cut(s) 773, 3387
Bbv12I GWGCWC 3 cut(s) 106, 255, 971
BceAI ACGGC 2 cut(s) 122, 166
BcgI CGANNNNNNTGC 2 cut(s) 2721, 2755
BciT130I CCWGG 7 cut(s) 296, 552, 1160, 1518, 2507, 3488, 3944
BciVI GTATCC 3 cut(s) 935, 1285, 1780
BclI TGATCA 5 cut(s) 1045, 1123, 2128, 2641, 3670
BcoDI GTCTC 4 cut(s) 366, 685, 1584, 1605
BcuI ACTAGT 1 cut(s) 971
BfmI CTRYAG 5 cut(s) 402, 825, 1779, 3522, 4050
BfoI RGCGCY 1 cut(s) 122
BfrI CTTAAG 1 cut(s) 3578
BfuAI ACCTGC 2 cut(s) 2187, 3628
BfuI GTATCC 3 cut(s) 935, 1285, 1780
BglII AGATCT 1 cut(s) 2023
BlnI CCTAGG 1 cut(s) 3208
BlpI GCTNAGC 1 cut(s) 3601
Bme1390I CCNGG 7 cut(s) 296, 552, 1160, 1518, 2507, 3488, 3944
Bme18I GGWCC 6 cut(s) 1156, 1979, 2056, 2176, 3506, 3920
BmgT120I GGNCC 8 cut(s) 1156, 1979, 2056, 2176, 3204, 3205, 3506, 3920
BmiI GGNNCC 6 cut(s) 790, 856, 1157, 1719, 3206, 3922
BmrFI CCNGG 7 cut(s) 296, 552, 1160, 1518, 2507, 3488, 3944
BmrI ACTGGG 1 cut(s) 915
BmsI GCATC 9 cut(s) 514, 1027, 1093, 1485, 1775, 2289, 2352, 3712, 4045
BmtI GCTAGC 1 cut(s) 3942
BmuI ACTGGG 1 cut(s) 915
BoxI GACNNNNGTC 1 cut(s) 3043
BpiI GAAGAC 2 cut(s) 773, 3387
BpmI CTGGAG 5 cut(s) 239, 1587, 2549, 2722, 3470
Bpu10I CCTNAGC 2 cut(s) 1007, 1507
Bpu1102I GCTNAGC 1 cut(s) 3601
BpuEI CTTGAG 2 cut(s) 337, 3285
Bsa29I ATCGAT 1 cut(s) 1812
BsaAI YACGTR 1 cut(s) 1100
BsaBI GATNNNNATC 1 cut(s) 882
BsaHI GRCGYC 1 cut(s) 910
BsaJI CCNNGG 9 cut(s) 78, 148, 189, 1517, 1736, 1959, 3208, 3431, 3487
BsaWI WCCGGW 1 cut(s) 462
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 434, 464
Bsc4I CCNNNNNNNGG 9 cut(s) 79, 244, 1151, 1512, 1714, 2512, 2809, 2903, 3257
Bse3DI GCAATG 4 cut(s) 537, 1259, 1382, 2073
Bse8I GATNNNNATC 1 cut(s) 882
BseBI CCWGG 7 cut(s) 296, 552, 1160, 1518, 2507, 3488, 3944
BseCI ATCGAT 1 cut(s) 1812
BseDI CCNNGG 9 cut(s) 78, 148, 189, 1517, 1736, 1959, 3208, 3431, 3487
BseJI GATNNNNATC 1 cut(s) 882
BseLI CCNNNNNNNGG 9 cut(s) 79, 244, 1151, 1512, 1714, 2512, 2809, 2903, 3257
BseMI GCAATG 4 cut(s) 537, 1259, 1382, 2073
BseMII CTCAG 5 cut(s) 1311, 1587, 2345, 2696, 3615
BseRI GAGGAG 9 cut(s) 102, 119, 163, 191, 205, 208, 320, 344, 455
BseSI GKGCMC 3 cut(s) 971, 3154, 3208
BseYI CCCAGC 2 cut(s) 1031, 3837
BsgI GTGCAG 3 cut(s) 852, 1616, 3453
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 8 cut(s) 83, 188, 841, 1457, 1516, 2722, 3206, 3570
BshNI GGYRCC 1 cut(s) 854
BshVI ATCGAT 1 cut(s) 1812
BsiHKAI GWGCWC 3 cut(s) 106, 255, 971
BsiSI CCGG 2 cut(s) 84, 463
BslFI GGGAC 4 cut(s) 1169, 2678, 2700, 3906
BslI CCNNNNNNNGG 9 cut(s) 79, 244, 1151, 1512, 1714, 2512, 2809, 2903, 3257
BsmAI GTCTC 4 cut(s) 366, 685, 1584, 1605
BsmFI GGGAC 4 cut(s) 1169, 2678, 2700, 3906
BsmI GAATGC 3 cut(s) 1033, 2914, 3798
BsnI GGCC 8 cut(s) 83, 188, 841, 1457, 1516, 2722, 3206, 3570
Bsp120I GGGCCC 1 cut(s) 3204
Bsp1286I GDGCHC 7 cut(s) 106, 255, 742, 971, 1015, 3154, 3208
Bsp1407I TGTACA 1 cut(s) 3101
Bsp1720I GCTNAGC 1 cut(s) 3601
Bsp19I CCATGG 2 cut(s) 78, 1959
BspANI GGCC 8 cut(s) 83, 188, 841, 1457, 1516, 2722, 3206, 3570
BspCNI CTCAG 5 cut(s) 1312, 1586, 2344, 2695, 3614
BspDI ATCGAT 1 cut(s) 1812
BspFNI CGCG 1 cut(s) 578
BspHI TCATGA 2 cut(s) 1210, 1243
BspLI GGNNCC 6 cut(s) 790, 856, 1157, 1719, 3206, 3922
BspMAI CTGCAG 1 cut(s) 3526
BspMI ACCTGC 2 cut(s) 2187, 3628
BspOI GCTAGC 1 cut(s) 3942
BspPI GGATC 5 cut(s) 878, 1712, 1725, 1727, 3242
BspT107I GGYRCC 1 cut(s) 854
BspTI CTTAAG 1 cut(s) 3578
BsrBI CCGCTC 1 cut(s) 1090
BsrDI GCAATG 4 cut(s) 537, 1259, 1382, 2073
BsrGI TGTACA 1 cut(s) 3101
BssECI CCNNGG 9 cut(s) 78, 148, 189, 1517, 1736, 1959, 3208, 3431, 3487
BssNI GRCGYC 1 cut(s) 910
BssSI CACGAG 1 cut(s) 2037
BssT1I CCWWGG 4 cut(s) 78, 189, 1959, 3208
Bst2BI CACGAG 1 cut(s) 2037
Bst2UI CCWGG 7 cut(s) 296, 552, 1160, 1518, 2507, 3488, 3944
Bst4CI ACNGT 9 cut(s) 377, 859, 1372, 1642, 1864, 3337, 3625, 3706, 4041
Bst6I CTCTTC 6 cut(s) 13, 44, 655, 1188, 2157, 3693
BstACI GRCGYC 1 cut(s) 910
BstAFI CTTAAG 1 cut(s) 3578
BstAPI GCANNNNNTGC 3 cut(s) 1388, 1754, 3722
BstAUI TGTACA 1 cut(s) 3101
BstBAI YACGTR 1 cut(s) 1100
BstC8I GCNNGC 6 cut(s) 94, 576, 1092, 1389, 3171, 3940
BstDEI CTNAG 9 cut(s) 1007, 1320, 1507, 1573, 2331, 2517, 2682, 2743, 3601
BstDSI CCRYGG 4 cut(s) 78, 148, 1736, 1959
BstENI CCTNNNNNAGG 4 cut(s) 1149, 1510, 2901, 3255
BstFNI CGCG 1 cut(s) 578
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 3 cut(s) 121, 2304, 3621
BstMAI GTCTC 4 cut(s) 366, 685, 1584, 1605
BstNI CCWGG 7 cut(s) 296, 552, 1160, 1518, 2507, 3488, 3944
BstNSI RCATGY 1 cut(s) 718
BstPAI GACNNNNGTC 1 cut(s) 3043
BstSCI CCNGG 7 cut(s) 294, 550, 1158, 1516, 2505, 3486, 3942
BstSFI CTRYAG 5 cut(s) 402, 825, 1779, 3522, 4050
BstSLI GKGCMC 3 cut(s) 971, 3154, 3208
BstUI CGCG 1 cut(s) 578
BstV2I GAAGAC 2 cut(s) 773, 3387
BstX2I RGATCY 2 cut(s) 1717, 2023
BstXI CCANNNNNNTGG 1 cut(s) 3804
BstYI RGATCY 2 cut(s) 1717, 2023
Bsu15I ATCGAT 1 cut(s) 1812
BsuI GTATCC 3 cut(s) 935, 1285, 1780
BsuRI GGCC 8 cut(s) 83, 188, 841, 1457, 1516, 2722, 3206, 3570
BsuTUI ATCGAT 1 cut(s) 1812
BtgI CCRYGG 4 cut(s) 78, 148, 1736, 1959
BtgZI GCGATG 1 cut(s) 338
BtsI GCAGTG 1 cut(s) 2517
BtsIMutI CAGTG 7 cut(s) 382, 984, 1860, 2517, 2667, 3048, 3333
BveI ACCTGC 2 cut(s) 2187, 3628
Cac8I GCNNGC 6 cut(s) 94, 576, 1092, 1389, 3171, 3940
CaiI CAGNNNCTG 4 cut(s) 1640, 1661, 1754, 2179
CciI TCATGA 2 cut(s) 1210, 1243
CfoI GCGC 3 cut(s) 121, 2304, 3621
Cfr13I GGNCC 8 cut(s) 1156, 1979, 2056, 2176, 3204, 3205, 3506, 3920
ClaI ATCGAT 1 cut(s) 1812
CseI GACGC 1 cut(s) 918
CsiI ACCWGGT 1 cut(s) 1158
Csp6I GTAC 5 cut(s) 341, 545, 712, 2431, 3102
CviQI GTAC 5 cut(s) 341, 545, 712, 2431, 3102
DdeI CTNAG 9 cut(s) 1007, 1320, 1507, 1573, 2331, 2517, 2682, 2743, 3601
DraI TTTAAA 1 cut(s) 180
EaeI YGGCCR 3 cut(s) 81, 1455, 2720
Eam1104I CTCTTC 6 cut(s) 13, 44, 655, 1188, 2157, 3693
EarI CTCTTC 6 cut(s) 13, 44, 655, 1188, 2157, 3693
EciI GGCGGA 3 cut(s) 114, 211, 1046
Ecl136II GAGCTC 2 cut(s) 104, 253
Eco130I CCWWGG 4 cut(s) 78, 189, 1959, 3208
Eco147I AGGCCT 1 cut(s) 3570
Eco24I GRGCYC 5 cut(s) 106, 255, 742, 1015, 3208
Eco47I GGWCC 6 cut(s) 1156, 1979, 2056, 2176, 3506, 3920
Eco47III AGCGCT 1 cut(s) 120
Eco53kI GAGCTC 2 cut(s) 104, 253
Eco57I CTGAAG 6 cut(s) 1413, 1617, 2004, 2181, 2529, 3084
Eco72I CACGTG 1 cut(s) 1100
EcoICRI GAGCTC 2 cut(s) 104, 253
EcoNI CCTNNNNNAGG 4 cut(s) 1149, 1510, 2901, 3255
EcoO109I RGGNCCY 6 cut(s) 1156, 2056, 2176, 3204, 3205, 3920
EcoRI GAATTC 1 cut(s) 747
EcoRII CCWGG 7 cut(s) 294, 550, 1158, 1516, 2505, 3486, 3942
EcoT14I CCWWGG 4 cut(s) 78, 189, 1959, 3208
EcoT38I GRGCYC 5 cut(s) 106, 255, 742, 1015, 3208
ErhI CCWWGG 4 cut(s) 78, 189, 1959, 3208
FaqI GGGAC 4 cut(s) 1169, 2678, 2700, 3906
FauI CCCGC 3 cut(s) 1083, 1696, 1783
FauNDI CATATG 3 cut(s) 598, 2238, 3934
FbaI TGATCA 5 cut(s) 1045, 1123, 2128, 2641, 3670
FblI GTMKAC 1 cut(s) 2563
FriOI GRGCYC 5 cut(s) 106, 255, 742, 1015, 3208
GlaI GCGC 3 cut(s) 120, 2303, 3620
GsaI CCCAGC 2 cut(s) 1035, 3841
GsuI CTGGAG 5 cut(s) 239, 1587, 2549, 2722, 3470
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 8 cut(s) 83, 188, 841, 1457, 1516, 2722, 3206, 3570
HapII CCGG 2 cut(s) 84, 463
HgaI GACGC 1 cut(s) 918
HhaI GCGC 3 cut(s) 121, 2304, 3621
Hin1I GRCGYC 1 cut(s) 910
Hin6I GCGC 3 cut(s) 119, 2302, 3619
HinP1I GCGC 3 cut(s) 119, 2302, 3619
HincII GTYRAC 4 cut(s) 142, 447, 1645, 2208
HindII GTYRAC 4 cut(s) 142, 447, 1645, 2208
HindIII AAGCTT 3 cut(s) 1835, 3800, 3957
HpaI GTTAAC 1 cut(s) 1645
HpaII CCGG 2 cut(s) 84, 463
Hpy166II GTNNAC 8 cut(s) 142, 447, 847, 969, 1645, 2208, 2564, 2931
Hpy8I GTNNAC 8 cut(s) 142, 447, 847, 969, 1645, 2208, 2564, 2931
Hpy99I CGWCG 2 cut(s) 629, 1728
HpyCH4III ACNGT 9 cut(s) 377, 859, 1372, 1642, 1864, 3337, 3625, 3706, 4041
HpyCH4IV ACGT 4 cut(s) 449, 1099, 2674, 2954
HpyF3I CTNAG 9 cut(s) 1007, 1320, 1507, 1573, 2331, 2517, 2682, 2743, 3601
HpySE526I ACGT 4 cut(s) 449, 1099, 2674, 2954
Hsp92I GRCGYC 1 cut(s) 910
HspAI GCGC 3 cut(s) 119, 2302, 3619
Ksp22I TGATCA 5 cut(s) 1045, 1123, 2128, 2641, 3670
KspAI GTTAAC 1 cut(s) 1645
LmnI GCTCC 6 cut(s) 89, 101, 204, 258, 1357, 2477
LweI GCATC 9 cut(s) 514, 1027, 1093, 1485, 1775, 2289, 2352, 3712, 4045
MabI ACCWGGT 1 cut(s) 1158
MaeII ACGT 4 cut(s) 449, 1099, 2674, 2954
MaeIII GTNAC 6 cut(s) 1345, 1703, 1879, 2677, 2834, 3370
MbiI CCGCTC 1 cut(s) 1090
MfeI CAATTG 4 cut(s) 798, 1928, 3293, 3717
MflI RGATCY 2 cut(s) 1717, 2023
MhlI GDGCHC 7 cut(s) 106, 255, 742, 971, 1015, 3154, 3208
MmeI TCCRAC 3 cut(s) 1607, 2065, 2827
MslI CAYNNNNRTG 4 cut(s) 296, 719, 3348, 3386
MspA1I CMGCKG 4 cut(s) 773, 824, 1691, 1778
MspCI CTTAAG 1 cut(s) 3578
MspI CCGG 2 cut(s) 84, 463
MspR9I CCNGG 7 cut(s) 296, 552, 1160, 1518, 2507, 3488, 3944
MunI CAATTG 4 cut(s) 798, 1928, 3293, 3717
Mva1269I GAATGC 3 cut(s) 1033, 2914, 3798
MvaI CCWGG 7 cut(s) 296, 552, 1160, 1518, 2507, 3488, 3944
MvnI CGCG 1 cut(s) 578
NcoI CCATGG 2 cut(s) 78, 1959
NdeI CATATG 3 cut(s) 598, 2238, 3934
NheI GCTAGC 1 cut(s) 3938
NlaIV GGNNCC 6 cut(s) 790, 856, 1157, 1719, 3206, 3922
NmeAIII GCCGAG 2 cut(s) 1513, 3412
NmuCI GTSAC 4 cut(s) 1345, 1703, 2677, 3370
NspI RCATGY 1 cut(s) 718
PagI TCATGA 2 cut(s) 1210, 1243
PceI AGGCCT 1 cut(s) 3570
PctI GAATGC 3 cut(s) 1033, 2914, 3798
PflMI CCANNNNNTGG 1 cut(s) 79
PmaCI CACGTG 1 cut(s) 1100
PmlI CACGTG 1 cut(s) 1100
Ppu21I YACGTR 1 cut(s) 1100
PpuMI RGGWCCY 4 cut(s) 1156, 2056, 2176, 3920
PshAI GACNNNNGTC 1 cut(s) 3043
PsiI TTATAA 1 cut(s) 3200
Psp124BI GAGCTC 2 cut(s) 106, 255
Psp1406I AACGTT 1 cut(s) 2954
Psp5II RGGWCCY 4 cut(s) 1156, 2056, 2176, 3920
Psp6I CCWGG 7 cut(s) 294, 550, 1158, 1516, 2505, 3486, 3942
PspCI CACGTG 1 cut(s) 1100
PspFI CCCAGC 2 cut(s) 1031, 3837
PspGI CCWGG 7 cut(s) 294, 550, 1158, 1516, 2505, 3486, 3942
PspN4I GGNNCC 6 cut(s) 790, 856, 1157, 1719, 3206, 3922
PspOMI GGGCCC 1 cut(s) 3204
PspPI GGNCC 8 cut(s) 1156, 1979, 2056, 2176, 3204, 3205, 3506, 3920
PspPPI RGGWCCY 4 cut(s) 1156, 2056, 2176, 3920
PsrI GAACNNNNNNTAC 2 cut(s) 2483, 2515
PstI CTGCAG 1 cut(s) 3526
PstNI CAGNNNCTG 4 cut(s) 1640, 1661, 1754, 2179
PsuI RGATCY 2 cut(s) 1717, 2023
PvuII CAGCTG 2 cut(s) 773, 824
RsaI GTAC 5 cut(s) 342, 546, 713, 2432, 3103
RsaNI GTAC 5 cut(s) 341, 545, 712, 2431, 3102
RseI CAYNNNNRTG 4 cut(s) 296, 719, 3348, 3386
SacI GAGCTC 2 cut(s) 106, 255
Sau96I GGNCC 8 cut(s) 1156, 1979, 2056, 2176, 3204, 3205, 3506, 3920
ScrFI CCNGG 7 cut(s) 296, 552, 1160, 1518, 2507, 3488, 3944
SduI GDGCHC 7 cut(s) 106, 255, 742, 971, 1015, 3154, 3208
SexAI ACCWGGT 1 cut(s) 1158
SfaNI GCATC 9 cut(s) 514, 1027, 1093, 1485, 1775, 2289, 2352, 3712, 4045
SfcI CTRYAG 5 cut(s) 402, 825, 1779, 3522, 4050
SinI GGWCC 6 cut(s) 1156, 1979, 2056, 2176, 3506, 3920
SmiMI CAYNNNNRTG 4 cut(s) 296, 719, 3348, 3386
SmlI CTYRAG 3 cut(s) 316, 3300, 3578
SmoI CTYRAG 3 cut(s) 316, 3300, 3578
SpeI ACTAGT 1 cut(s) 971
SseBI AGGCCT 1 cut(s) 3570
SspI AATATT 3 cut(s) 673, 3187, 3550
SstI GAGCTC 2 cut(s) 106, 255
StuI AGGCCT 1 cut(s) 3570
StyD4I CCNGG 7 cut(s) 294, 550, 1158, 1516, 2505, 3486, 3942
StyI CCWWGG 4 cut(s) 78, 189, 1959, 3208
TaaI ACNGT 9 cut(s) 377, 859, 1372, 1642, 1864, 3337, 3625, 3706, 4041
TaiI ACGT 4 cut(s) 452, 1102, 2677, 2957
TaqII GACCGA 1 cut(s) 1098
TatI WGTACW 3 cut(s) 340, 544, 3101
TauI GCSGC 5 cut(s) 99, 137, 227, 1501, 2722
TscAI CASTG 7 cut(s) 382, 984, 1867, 2517, 2674, 3048, 3340
TseFI GTSAC 4 cut(s) 1345, 1703, 2677, 3370
Tsp45I GTSAC 4 cut(s) 1345, 1703, 2677, 3370
TspDTI ATGAA 9 cut(s) 1040, 1260, 2364, 2508, 2759, 3366, 3723, 4017, 4053
TspGWI ACGGA 2 cut(s) 1753, 2295
TspRI CASTG 7 cut(s) 382, 984, 1867, 2517, 2674, 3048, 3340
Van91I CCANNNNNTGG 1 cut(s) 79
Vha464I CTTAAG 1 cut(s) 3578
VneI GTGCAC 1 cut(s) 967
VpaK11BI GGWCC 6 cut(s) 1156, 1979, 2056, 2176, 3506, 3920
XagI CCTNNNNNAGG 4 cut(s) 1149, 1510, 2901, 3255
XapI RAATTY 8 cut(s) 584, 747, 757, 1422, 2858, 2992, 3007, 3458
XbaI TCTAGA 1 cut(s) 3968
XceI RCATGY 1 cut(s) 718
XmaJI CCTAGG 1 cut(s) 3208
XmiI GTMKAC 1 cut(s) 2563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.