FvH4_6g01370

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0018854
Physical Location & Seq
Reverse (-)
762872 .. 764920
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01370.t1

Sequence Viewer

Length: 1134 bp
ATGATCCAATTAATGGCCACCTCTTCATCATCAAATTCAGTGATTACAATATGTCGTCTGATCTTTCTGGCATTGTTGGGAACCATTTACCTTGCTCCTCAAGCGGAAGCGGCTCGAGCTTTCTTTGTTTTTGGTGATTCACTGGTCGATAACGGCAACAACAACTATTTGATCACCACTGCTCGTGCCGACGCTTACCCCTACGGCATTGATTCTCCGACGCACCGGCCCTCCGGCCGATTCTCCAATGGCCTTAACATTGCGGACATCATCAGTCAGAAAATTGGGTCACCTGAACCGCCATTGCCATACTTGAGTCCGCAGCTAAATGGAAGAAGGCTGCTTATTGGTGCCAACTTCGCTTCAGCTGGGGTTGGAATTCTCAATGACACCGGATTTCAGTTTCTAAATATACTCCGAATGCCTACACAACTAGCGTACTTTCGAGATTACCAGAGAAGAGTGCAAGCTCTAATCGGACGTGACCAAACCAAAAGACTCATAGAGCAATCCCTCGTCCTCATCACCGTCGGCGGCAACGATTTCGTCAACAACTACTATTTGGGCCCTTCCTCCGCCCGATCTCGCCAACTTCCTCTCCCAAGATATGTCAGTTATCTCATCTCCGAGTACGAGAAGCTTTTGATGAACTTGTATAAGCTAGGACCATGTAGGATTCTGGTGACCGGTACCGGAGCAATGGGATGTGTTCCCGGAGAATTGGCGACTAGAGCCACCAACGGTGGATGTTCAGCTGAAGTTCAACGAGCCGGTGCCTTATACAATCCGCAACTCGATAAAATGTTGAAGGGACTCAACAAGAAACTCGGGAAAGACGTTTTCATCTCTGCAAATAATGGACAAATGCATTTGGATTTCATCAACAACCCTCGAGCATTTGGGTTTACTAATTCCAAGGTCGCGTGTTGCGGGCAAGGACCCTATAATGGAGTTGGTCTCTGCACATTTCTGTCCAACTTGTGCCCTAACAGAGCTCTCTATGCGTTTTGGGATGCCTTCCACCCATCTGAAAAGGCTAATAGAATTATTGTGCAGCAGATGATTACTGGCTCCATCCGCTACATGAGTCCCATGAATCTCAGCACAATCATGGCCTTGGACTCTGCTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

378

Amino Acids

41.24

Weight (kDa)

9.37

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 352 9.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018854)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01370
malus_domestica MD04G1234800.v1.1
rosa_chinensis RchiOBHm_Chr3g0448941
rosa_laevigata RLG00000025864
rosa_multiflora Rmu_sc0003116.1_g000015
rosa_roxburghii Rroxscaffold_6G00427120
rosa_rugosa Rorug02G0617200
rosa_samantha Rh3CG014800
rosa_wichuraiana Rw3G001170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 689
AccB1I GGYRCC 3 cut(s) 350, 689, 773
AccB7I CCANNNNNTGG 1 cut(s) 13
AccII CGCG 1 cut(s) 923
AcoI YGGCCR 2 cut(s) 15, 235
AcsI RAATTY 2 cut(s) 34, 378
AcuI CTGAAG 2 cut(s) 348, 777
AfaI GTAC 3 cut(s) 440, 632, 691
AfiI CCNNNNNNNGG 2 cut(s) 13, 947
AgeI ACCGGT 1 cut(s) 686
AgsI TTSAA 2 cut(s) 764, 808
AjiI CACGTC 1 cut(s) 482
AluBI AGCT 8 cut(s) 119, 325, 368, 470, 640, 661, 755, 995
AluI AGCT 8 cut(s) 119, 325, 368, 470, 640, 661, 755, 995
Alw21I GWGCWC 1 cut(s) 997
Alw26I GTCTC 1 cut(s) 962
Ama87I CYCGRG 3 cut(s) 114, 827, 891
AoxI GGCC 6 cut(s) 15, 227, 235, 250, 565, 1113
ApaI GGGCCC 1 cut(s) 569
ApeKI GCWGC 3 cut(s) 322, 340, 1054
ApoI RAATTY 2 cut(s) 34, 378
AseI ATTAAT 1 cut(s) 11
AsiGI ACCGGT 1 cut(s) 686
Asp718I GGTACC 1 cut(s) 689
AspS9I GGNCC 5 cut(s) 228, 565, 566, 665, 938
AsuC2I CCSGG 1 cut(s) 714
AsuHPI GGTGA 5 cut(s) 146, 166, 282, 517, 694
AvaI CYCGRG 3 cut(s) 114, 827, 891
AvaII GGWCC 2 cut(s) 665, 938
BaeGI GKGCMC 2 cut(s) 569, 986
BalI TGGCCA 1 cut(s) 17
BanI GGYRCC 3 cut(s) 350, 689, 773
BanII GRGCYC 2 cut(s) 569, 997
BauI CACGAG 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 997
BbvI GCAGC 3 cut(s) 327, 334, 1066
BccI CCATC 2 cut(s) 1033, 1082
BceAI ACGGC 2 cut(s) 169, 220
BcgI CGANNNNNNTGC 2 cut(s) 526, 560
BclI TGATCA 1 cut(s) 171
BcnI CCSGG 1 cut(s) 714
BcoDI GTCTC 1 cut(s) 962
BfaI CTAG 3 cut(s) 434, 662, 729
BisI GCNGC 5 cut(s) 111, 323, 341, 535, 1055
BlsI GCNGC 5 cut(s) 112, 324, 342, 536, 1056
Bme1390I CCNGG 1 cut(s) 714
Bme18I GGWCC 2 cut(s) 665, 938
BmeT110I CYCGRG 3 cut(s) 114, 827, 891
BmgBI CACGTC 1 cut(s) 482
BmgT120I GGNCC 5 cut(s) 228, 565, 566, 665, 938
BmiI GGNNCC 7 cut(s) 82, 352, 567, 691, 775, 940, 1072
BmrFI CCNGG 1 cut(s) 714
BmsI GCATC 1 cut(s) 1003
BplI GAGNNNNNCTC 2 cut(s) 942, 974
BpuEI CTTGAG 2 cut(s) 84, 334
BpuMI CCSGG 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 962
BsaJI CCNNGG 2 cut(s) 915, 1116
BsaWI WCCGGW 3 cut(s) 392, 686, 692
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 582, 612
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 947
Bse118I RCCGGY 3 cut(s) 225, 686, 770
Bse1I ACTGG 2 cut(s) 147, 1072
Bse3DI GCAATG 3 cut(s) 258, 302, 705
BseDI CCNNGG 2 cut(s) 915, 1116
BseGI GGATG 4 cut(s) 710, 752, 1018, 1074
BseLI CCNNNNNNNGG 2 cut(s) 13, 947
BseMI GCAATG 3 cut(s) 258, 302, 705
BseMII CTCAG 1 cut(s) 1114
BseNI ACTGG 2 cut(s) 147, 1072
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 2 cut(s) 569, 986
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 3 cut(s) 327, 334, 1066
BseYI CCCAGC 1 cut(s) 368
BsgI GTGCAG 2 cut(s) 946, 1073
Bsh1236I CGCG 1 cut(s) 923
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 6 cut(s) 17, 229, 237, 252, 567, 1115
BshNI GGYRCC 3 cut(s) 350, 689, 773
BshTI ACCGGT 1 cut(s) 686
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 997
BsiHKCI CYCGRG 3 cut(s) 114, 827, 891
BsiSI CCGG 7 cut(s) 226, 234, 393, 687, 693, 714, 771
BslFI GGGAC 2 cut(s) 825, 1074
BslI CCNNNNNNNGG 2 cut(s) 13, 947
BsmAI GTCTC 1 cut(s) 962
BsmFI GGGAC 2 cut(s) 825, 1074
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 6 cut(s) 17, 229, 237, 252, 567, 1115
Bso31I GGTCTC 1 cut(s) 962
BsoBI CYCGRG 3 cut(s) 114, 827, 891
Bsp120I GGGCCC 1 cut(s) 565
Bsp1286I GDGCHC 3 cut(s) 569, 986, 997
Bsp143I GATC 4 cut(s) 3, 60, 171, 581
BspANI GGCC 6 cut(s) 17, 229, 237, 252, 567, 1115
BspCNI CTCAG 1 cut(s) 1113
BspFNI CGCG 1 cut(s) 923
BspLI GGNNCC 7 cut(s) 82, 352, 567, 691, 775, 940, 1072
BspT107I GGYRCC 3 cut(s) 350, 689, 773
BspTNI GGTCTC 1 cut(s) 962
BsrDI GCAATG 3 cut(s) 258, 302, 705
BsrFI RCCGGY 3 cut(s) 225, 686, 770
BsrI ACTGG 2 cut(s) 147, 1072
BssAI RCCGGY 3 cut(s) 225, 686, 770
BssECI CCNNGG 2 cut(s) 915, 1116
BssMI GATC 4 cut(s) 3, 60, 171, 581
BssSI CACGAG 1 cut(s) 183
BssT1I CCWWGG 2 cut(s) 915, 1116
Bst2BI CACGAG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 529, 743
Bst6I CTCTTC 2 cut(s) 28, 454
BstC8I GCNNGC 2 cut(s) 468, 932
BstDEI CTNAG 1 cut(s) 1100
BstEII GGTNACC 2 cut(s) 288, 682
BstF5I GGATG 4 cut(s) 710, 752, 1018, 1074
BstFNI CGCG 1 cut(s) 923
BstKTI GATC 4 cut(s) 6, 63, 174, 584
BstMAI GTCTC 1 cut(s) 962
BstMBI GATC 4 cut(s) 3, 60, 171, 581
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 7 cut(s) 101, 110, 116, 359, 731, 1001, 1077
BstPI GGTNACC 2 cut(s) 288, 682
BstSCI CCNGG 1 cut(s) 712
BstSLI GKGCMC 2 cut(s) 569, 986
BstUI CGCG 1 cut(s) 923
BstV1I GCAGC 3 cut(s) 327, 334, 1066
BstZI CGGCCG 1 cut(s) 235
BsuRI GGCC 6 cut(s) 17, 229, 237, 252, 567, 1115
BtrI CACGTC 1 cut(s) 482
BtsCI GGATG 4 cut(s) 710, 752, 1018, 1074
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 3 cut(s) 45, 140, 177
Cac8I GCNNGC 2 cut(s) 468, 932
Cfr10I RCCGGY 3 cut(s) 225, 686, 770
Cfr13I GGNCC 5 cut(s) 228, 565, 566, 665, 938
CseI GACGC 2 cut(s) 200, 229
Csp6I GTAC 3 cut(s) 439, 631, 690
CspAI ACCGGT 1 cut(s) 686
CviAII CATG 4 cut(s) 669, 1084, 1093, 1111
CviQI GTAC 3 cut(s) 439, 631, 690
DdeI CTNAG 1 cut(s) 1100
DpnI GATC 4 cut(s) 5, 62, 173, 583
DpnII GATC 4 cut(s) 3, 60, 171, 581
EaeI YGGCCR 2 cut(s) 15, 235
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 2 cut(s) 28, 454
EarI CTCTTC 2 cut(s) 28, 454
EciI GGCGGA 1 cut(s) 565
Ecl136II GAGCTC 1 cut(s) 995
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 2 cut(s) 915, 1116
Eco24I GRGCYC 2 cut(s) 569, 997
Eco31I GGTCTC 1 cut(s) 962
Eco47I GGWCC 2 cut(s) 665, 938
Eco52I CGGCCG 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 995
Eco57I CTGAAG 2 cut(s) 348, 777
Eco88I CYCGRG 3 cut(s) 114, 827, 891
Eco91I GGTNACC 2 cut(s) 288, 682
EcoICRI GAGCTC 1 cut(s) 995
EcoO109I RGGNCCY 2 cut(s) 566, 938
EcoO65I GGTNACC 2 cut(s) 288, 682
EcoRI GAATTC 1 cut(s) 378
EcoT14I CCWWGG 2 cut(s) 915, 1116
EcoT22I ATGCAT 1 cut(s) 870
EcoT38I GRGCYC 2 cut(s) 569, 997
ErhI CCWWGG 2 cut(s) 915, 1116
FaeI CATG 4 cut(s) 672, 1087, 1096, 1114
FaqI GGGAC 2 cut(s) 825, 1074
FatI CATG 4 cut(s) 668, 1083, 1092, 1110
FauI CCCGC 1 cut(s) 923
FbaI TGATCA 1 cut(s) 171
Fnu4HI GCNGC 5 cut(s) 111, 323, 341, 535, 1055
FokI GGATG 4 cut(s) 717, 759, 1025, 1061
FriOI GRGCYC 2 cut(s) 569, 997
Fsp4HI GCNGC 5 cut(s) 111, 323, 341, 535, 1055
FspBI CTAG 3 cut(s) 434, 662, 729
GluI GCNGC 5 cut(s) 111, 323, 341, 535, 1055
GsaI CCCAGC 1 cut(s) 372
HaeIII GGCC 6 cut(s) 17, 229, 237, 252, 567, 1115
HapII CCGG 7 cut(s) 226, 234, 393, 687, 693, 714, 771
HgaI GACGC 2 cut(s) 200, 229
Hin1II CATG 4 cut(s) 672, 1087, 1096, 1114
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 638
HpaII CCGG 7 cut(s) 226, 234, 393, 687, 693, 714, 771
HphI GGTGA 5 cut(s) 146, 166, 282, 517, 694
Hpy166II GTNNAC 2 cut(s) 550, 906
Hpy188I TCNGA 7 cut(s) 60, 219, 279, 419, 479, 628, 1030
Hpy188III TCNNGA 2 cut(s) 446, 829
Hpy8I GTNNAC 2 cut(s) 550, 906
Hpy99I CGWCG 3 cut(s) 194, 223, 533
HpyAV CCTTC 4 cut(s) 330, 579, 802, 1027
HpyCH4III ACNGT 2 cut(s) 529, 743
HpyCH4IV ACGT 2 cut(s) 481, 837
HpyCH4V TGCA 5 cut(s) 466, 851, 868, 963, 1054
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 110, 116, 359, 731, 1001, 1077
HpyF3I CTNAG 1 cut(s) 1100
HpySE526I ACGT 2 cut(s) 481, 837
Hsp92II CATG 4 cut(s) 672, 1087, 1096, 1114
KpnI GGTACC 1 cut(s) 693
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 4 cut(s) 3, 60, 171, 581
LmnI GCTCC 3 cut(s) 100, 695, 1076
Lsp1109I GCAGC 3 cut(s) 327, 334, 1066
LweI GCATC 1 cut(s) 1003
MaeI CTAG 3 cut(s) 434, 662, 729
MaeII ACGT 2 cut(s) 481, 837
MaeIII GTNAC 3 cut(s) 288, 482, 682
MalI GATC 4 cut(s) 5, 62, 173, 583
MboI GATC 4 cut(s) 3, 60, 171, 581
MboII GAAGA 3 cut(s) 15, 345, 471
MhlI GDGCHC 3 cut(s) 569, 986, 997
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 7 cut(s) 8, 34, 282, 378, 719, 910, 1044
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 5 cut(s) 325, 492, 807, 1096, 1115
MmeI TCCRAC 3 cut(s) 242, 355, 999
MnlI CCTC 8 cut(s) 31, 108, 241, 524, 530, 583, 606, 900
Mox20I TGGCCA 1 cut(s) 17
Mph1103I ATGCAT 1 cut(s) 870
MscI TGGCCA 1 cut(s) 17
MseI TTAA 2 cut(s) 11, 255
MslI CAYNNNNRTG 1 cut(s) 1109
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 2 cut(s) 368, 755
MspI CCGG 7 cut(s) 226, 234, 393, 687, 693, 714, 771
MspR9I CCNGG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 426
MvnI CGCG 1 cut(s) 923
MwoI GCNNNNNNNGC 7 cut(s) 101, 110, 116, 359, 731, 1001, 1077
NciI CCSGG 1 cut(s) 714
NdeII GATC 4 cut(s) 3, 60, 171, 581
NlaIII CATG 4 cut(s) 672, 1087, 1096, 1114
NlaIV GGNNCC 7 cut(s) 82, 352, 567, 691, 775, 940, 1072
NmuCI GTSAC 3 cut(s) 288, 482, 682
NsiI ATGCAT 1 cut(s) 870
PaeR7I CTCGAG 2 cut(s) 114, 891
PcsI WCGNNNNNNNCGW 2 cut(s) 537, 834
PctI GAATGC 1 cut(s) 426
PfeI GAWTC 5 cut(s) 137, 212, 240, 676, 1096
PflMI CCANNNNNTGG 1 cut(s) 13
PfoI TCCNGGA 1 cut(s) 712
PinAI ACCGGT 1 cut(s) 686
PkrI GCNGC 5 cut(s) 112, 324, 342, 536, 1056
PleI GAGTC 5 cut(s) 324, 492, 807, 1095, 1115
PpsI GAGTC 5 cut(s) 324, 492, 807, 1095, 1115
PpuMI RGGWCCY 1 cut(s) 938
PshBI ATTAAT 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 997
Psp5II RGGWCCY 1 cut(s) 938
PspEI GGTNACC 2 cut(s) 288, 682
PspFI CCCAGC 1 cut(s) 368
PspN4I GGNNCC 7 cut(s) 82, 352, 567, 691, 775, 940, 1072
PspOMI GGGCCC 1 cut(s) 565
PspPI GGNCC 5 cut(s) 228, 565, 566, 665, 938
PspPPI RGGWCCY 1 cut(s) 938
PspXI VCTCGAGB 2 cut(s) 114, 891
PvuII CAGCTG 2 cut(s) 368, 755
RsaI GTAC 3 cut(s) 440, 632, 691
RsaNI GTAC 3 cut(s) 439, 631, 690
RseI CAYNNNNRTG 1 cut(s) 1109
SacI GAGCTC 1 cut(s) 997
SaqAI TTAA 2 cut(s) 11, 255
SatI GCNGC 5 cut(s) 111, 323, 341, 535, 1055
Sau3AI GATC 4 cut(s) 3, 60, 171, 581
Sau96I GGNCC 5 cut(s) 228, 565, 566, 665, 938
SchI GAGTC 5 cut(s) 325, 492, 807, 1096, 1115
ScrFI CCNGG 1 cut(s) 714
SduI GDGCHC 3 cut(s) 569, 986, 997
SfaNI GCATC 1 cut(s) 1003
Sfr274I CTCGAG 2 cut(s) 114, 891
SinI GGWCC 2 cut(s) 665, 938
SlaI CTCGAG 2 cut(s) 114, 891
SmiMI CAYNNNNRTG 1 cut(s) 1109
SmlI CTYRAG 4 cut(s) 99, 114, 313, 891
SmoI CTYRAG 4 cut(s) 99, 114, 313, 891
Sse9I AATT 7 cut(s) 8, 34, 282, 378, 719, 910, 1044
SspMI CTAG 3 cut(s) 434, 662, 729
SstI GAGCTC 1 cut(s) 997
StyD4I CCNGG 1 cut(s) 712
StyI CCWWGG 2 cut(s) 915, 1116
TaaI ACNGT 2 cut(s) 529, 743
TaiI ACGT 2 cut(s) 484, 840
TaqI TCGA 5 cut(s) 115, 147, 445, 795, 892
TasI AATT 7 cut(s) 8, 34, 282, 378, 719, 910, 1044
TauI GCSGC 2 cut(s) 113, 537
TfiI GAWTC 5 cut(s) 137, 212, 240, 676, 1096
Tru1I TTAA 2 cut(s) 11, 255
Tru9I TTAA 2 cut(s) 11, 255
TscAI CASTG 3 cut(s) 45, 147, 184
TseFI GTSAC 3 cut(s) 288, 482, 682
TseI GCWGC 3 cut(s) 322, 340, 1054
Tsp45I GTSAC 3 cut(s) 288, 482, 682
TspDTI ATGAA 5 cut(s) 15, 662, 832, 868, 1109
TspRI CASTG 3 cut(s) 45, 147, 184
Van91I CCANNNNNTGG 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 665, 938
VspI ATTAAT 1 cut(s) 11
XapI RAATTY 2 cut(s) 34, 378
XhoI CTCGAG 2 cut(s) 114, 891
XspI CTAG 3 cut(s) 434, 662, 729
Zsp2I ATGCAT 1 cut(s) 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.