RLG00000025864

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018854
Physical Location & Seq
Forward (+)
48773406 .. 48774890
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025864

Sequence Viewer

Length: 1122 bp
ATGGCCACCTATTCATTATCAAATTCAGTGACGACTACATGTTGTCTGATCTTTTTGGCATTCTTGGGAACCATTTACCTTGCTCCTCAAGCAGAAGCGGCCAGAGCTTTCTTTGTTTTTGGTGATTCACTGGTTGATAATGGCAACAACAACTATCTGATCACCAGTGCTCGTGCCGACTCTTACCCCTACGGCATTGATTCTCCGACACACCGCGCCTCCGGCAGATTCTCCAATGGCCTTAACATTGCGGACATCATCAGTCAGAAAATTGGGTCAGTAGAACCGCCATTGCCATACTTGAGTCCGGATCTAAATGGAAGAAGGCTACTTATTGGTGCCAACTTCGCTTCAGCCGGGGTTGGAATTCTCAATGACACCGGAATTCAGTTTCTAAATATACTCCGAATGCCTGTACAACTAGCGTACTTCCAAGAATACCAGAGAAGAGTGCAGGCTCTCATCGGACGTGACCAAACCAAAAGACTCATAGAACAATCCCTCGTCCTTATCACTGTCGGCGGCAATGATTTCGTCAACAACTACTTTTTGGGCCCTTCCTCCGCTCGATCTCGCCAACTTCCTCTCCCAAGATATGTCAAGTATGTCATCTCCGAGTACGAGAAACTTTTGATGAACCTGTATAAGCTGGGACCACGTAGGATTCTGGTGACCGGTACCGGAGCAATGGGCTGTGTCCCCGGAGAATTGGCGACTAGAGCCACGAATGGTGGATGCGCAGCTGAAGTTCAACGAGCCGGTGCCTTGTACAATCCGCAACTCGATCAAATGTTGAAAGGACTCAACAAGAAACTTGGGAAAGACGTTTTCATTTCTGCAAATAATGGACAAATGCATCTGGATTTCATCAACAACCCTCGATCATTTGGGTTTACTAATTCCAAGGTAGCATGTTGCGGGCAAGGACCCTACAATGGAGTTGGTCTCTGCACATTTCTGTCCAACTTGTGCCCTAACAGAGCTCTCTATGCGTTTTGGGACGCCTTCCACCCATCTGAGAAGGCTAATAGATTTATTGTGCAGCAGATGAATAGCGGCTCAATTCGCTACATGAGCCCCATGAACCTCAGCACAATCATGGCCCTGGACTCTACTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

374

Amino Acids

40.85

Weight (kDa)

9.22

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 348 7.9e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018854)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01370
malus_domestica MD04G1234800.v1.1
rosa_chinensis RchiOBHm_Chr3g0448941
rosa_laevigata RLG00000025864
rosa_multiflora Rmu_sc0003116.1_g000015
rosa_roxburghii Rroxscaffold_6G00427120
rosa_rugosa Rorug02G0617200
rosa_samantha Rh3CG014800
rosa_wichuraiana Rw3G001170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 739
Acc65I GGTACC 1 cut(s) 677
AccB1I GGYRCC 3 cut(s) 338, 677, 761
AccBSI CCGCTC 1 cut(s) 566
AccII CGCG 1 cut(s) 216
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 9 cut(s) 98, 214, 251, 287, 522, 564, 776, 918, 1056
AclWI GGATC 1 cut(s) 318
AcoI YGGCCR 2 cut(s) 3, 99
AcsI RAATTY 3 cut(s) 22, 366, 384
AcuI CTGAAG 2 cut(s) 336, 765
AcyI GRCGYC 1 cut(s) 1002
AfaI GTAC 5 cut(s) 417, 428, 620, 679, 770
AfiI CCNNNNNNNGG 1 cut(s) 935
AflIII ACRYGT 1 cut(s) 38
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 2 cut(s) 752, 796
AjiI CACGTC 1 cut(s) 470
AjnI CCWGG 1 cut(s) 1104
AluBI AGCT 4 cut(s) 107, 649, 743, 983
AluI AGCT 4 cut(s) 107, 649, 743, 983
Alw21I GWGCWC 2 cut(s) 172, 985
Alw26I GTCTC 1 cut(s) 950
AlwI GGATC 1 cut(s) 318
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 5 cut(s) 3, 99, 238, 553, 1101
ApaI GGGCCC 1 cut(s) 557
ApeKI GCWGC 2 cut(s) 740, 1042
ApoI RAATTY 3 cut(s) 22, 366, 384
AsiGI ACCGGT 1 cut(s) 674
Asp718I GGTACC 1 cut(s) 677
AspLEI GCGC 2 cut(s) 218, 740
AspS9I GGNCC 5 cut(s) 553, 554, 653, 926, 1102
AsuC2I CCSGG 2 cut(s) 358, 702
AsuHPI GGTGA 3 cut(s) 134, 154, 682
AvaII GGWCC 2 cut(s) 653, 926
BaeGI GKGCMC 2 cut(s) 557, 974
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 3 cut(s) 338, 677, 761
BanII GRGCYC 3 cut(s) 557, 985, 1079
BauI CACGAG 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 172, 985
BbvCI CCTCAGC 1 cut(s) 1088
BbvI GCAGC 2 cut(s) 752, 1054
BccI CCATC 1 cut(s) 1021
BceAI ACGGC 1 cut(s) 208
BcgI CGANNNNNNTGC 2 cut(s) 514, 548
BciT130I CCWGG 1 cut(s) 1106
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 2 cut(s) 358, 702
BcoDI GTCTC 1 cut(s) 950
BfaI CTAG 2 cut(s) 422, 717
BisI GCNGC 5 cut(s) 99, 523, 741, 1043, 1057
BlsI GCNGC 5 cut(s) 100, 524, 742, 1044, 1058
Bme1390I CCNGG 3 cut(s) 358, 702, 1106
Bme18I GGWCC 2 cut(s) 653, 926
BmgBI CACGTC 1 cut(s) 470
BmgT120I GGNCC 5 cut(s) 553, 554, 653, 926, 1102
BmiI GGNNCC 7 cut(s) 70, 340, 555, 654, 679, 763, 928
BmrFI CCNGG 3 cut(s) 358, 702, 1106
BmsI GCATC 2 cut(s) 725, 865
BplI GAGNNNNNCTC 2 cut(s) 930, 962
Bpu10I CCTNAGC 1 cut(s) 1088
BpuEI CTTGAG 2 cut(s) 72, 322
BpuMI CCSGG 2 cut(s) 358, 702
BsaAI YACGTR 1 cut(s) 659
BsaHI GRCGYC 1 cut(s) 1002
BsaI GGTCTC 1 cut(s) 950
BsaJI CCNNGG 4 cut(s) 357, 700, 903, 1104
BsaWI WCCGGW 4 cut(s) 307, 380, 674, 680
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 570, 600
Bsc4I CCNNNNNNNGG 1 cut(s) 935
Bse118I RCCGGY 2 cut(s) 674, 758
Bse1I ACTGG 2 cut(s) 135, 165
Bse3DI GCAATG 4 cut(s) 246, 290, 532, 693
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 1 cut(s) 1106
BseDI CCNNGG 4 cut(s) 357, 700, 903, 1104
BseGI GGATG 1 cut(s) 740
BseLI CCNNNNNNNGG 1 cut(s) 935
BseMI GCAATG 4 cut(s) 246, 290, 532, 693
BseMII CTCAG 2 cut(s) 1008, 1102
BseNI ACTGG 2 cut(s) 135, 165
BseRI GAGGAG 1 cut(s) 75
BseSI GKGCMC 2 cut(s) 557, 974
BseXI GCAGC 2 cut(s) 752, 1054
BseYI CCCAGC 1 cut(s) 649
BsgI GTGCAG 3 cut(s) 473, 934, 1061
Bsh1236I CGCG 1 cut(s) 216
BshFI GGCC 5 cut(s) 5, 101, 240, 555, 1103
BshNI GGYRCC 3 cut(s) 338, 677, 761
BshTI ACCGGT 1 cut(s) 674
BsiHKAI GWGCWC 2 cut(s) 172, 985
BsiSI CCGG 8 cut(s) 222, 308, 357, 381, 675, 681, 702, 759
BslFI GGGAC 3 cut(s) 666, 683, 1013
BslI CCNNNNNNNGG 1 cut(s) 935
BsmAI GTCTC 1 cut(s) 950
BsmFI GGGAC 3 cut(s) 666, 683, 1013
BsmI GAATGC 2 cut(s) 59, 414
BsnI GGCC 5 cut(s) 5, 101, 240, 555, 1103
Bso31I GGTCTC 1 cut(s) 950
Bsp120I GGGCCC 1 cut(s) 553
Bsp1286I GDGCHC 5 cut(s) 172, 557, 974, 985, 1079
Bsp13I TCCGGA 1 cut(s) 307
Bsp1407I TGTACA 2 cut(s) 415, 768
Bsp143I GATC 6 cut(s) 48, 159, 310, 569, 784, 881
BspACI CCGC 9 cut(s) 98, 214, 251, 287, 522, 564, 776, 918, 1056
BspANI GGCC 5 cut(s) 5, 101, 240, 555, 1103
BspCNI CTCAG 2 cut(s) 1009, 1101
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 1 cut(s) 216
BspLI GGNNCC 7 cut(s) 70, 340, 555, 654, 679, 763, 928
BspPI GGATC 1 cut(s) 318
BspT107I GGYRCC 3 cut(s) 338, 677, 761
BspTNI GGTCTC 1 cut(s) 950
BsrBI CCGCTC 1 cut(s) 566
BsrDI GCAATG 4 cut(s) 246, 290, 532, 693
BsrFI RCCGGY 2 cut(s) 674, 758
BsrGI TGTACA 2 cut(s) 415, 768
BsrI ACTGG 2 cut(s) 135, 165
BssAI RCCGGY 2 cut(s) 674, 758
BssECI CCNNGG 4 cut(s) 357, 700, 903, 1104
BssMI GATC 6 cut(s) 48, 159, 310, 569, 784, 881
BssNI GRCGYC 1 cut(s) 1002
BssSI CACGAG 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 903
Bst2BI CACGAG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 1106
Bst4CI ACNGT 1 cut(s) 517
Bst6I CTCTTC 1 cut(s) 442
BstACI GRCGYC 1 cut(s) 1002
BstAUI TGTACA 2 cut(s) 415, 768
BstBAI YACGTR 1 cut(s) 659
BstC8I GCNNGC 2 cut(s) 456, 920
BstDEI CTNAG 2 cut(s) 1017, 1088
BstEII GGTNACC 1 cut(s) 670
BstF5I GGATG 1 cut(s) 740
BstFNI CGCG 1 cut(s) 216
BstHHI GCGC 2 cut(s) 218, 740
BstKTI GATC 6 cut(s) 51, 162, 313, 572, 787, 884
BstMAI GTCTC 1 cut(s) 950
BstMBI GATC 6 cut(s) 48, 159, 310, 569, 784, 881
BstMWI GCNNNNNNNGC 9 cut(s) 89, 98, 104, 222, 347, 719, 989, 1065, 1074
BstNI CCWGG 1 cut(s) 1106
BstNSI RCATGY 2 cut(s) 42, 915
BstPI GGTNACC 1 cut(s) 670
BstSCI CCNGG 3 cut(s) 356, 700, 1104
BstSLI GKGCMC 2 cut(s) 557, 974
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 2 cut(s) 752, 1054
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BsuRI GGCC 5 cut(s) 5, 101, 240, 555, 1103
BtrI CACGTC 1 cut(s) 470
BtsCI GGATG 1 cut(s) 740
BtsIMutI CAGTG 4 cut(s) 33, 128, 172, 513
Cac8I GCNNGC 2 cut(s) 456, 920
CfoI GCGC 2 cut(s) 218, 740
Cfr10I RCCGGY 2 cut(s) 674, 758
Cfr13I GGNCC 5 cut(s) 553, 554, 653, 926, 1102
CseI GACGC 1 cut(s) 1010
Csp6I GTAC 5 cut(s) 416, 427, 619, 678, 769
CspAI ACCGGT 1 cut(s) 674
CviAII CATG 5 cut(s) 39, 912, 1072, 1081, 1099
CviQI GTAC 5 cut(s) 416, 427, 619, 678, 769
DdeI CTNAG 2 cut(s) 1017, 1088
DpnI GATC 6 cut(s) 50, 161, 312, 571, 786, 883
DpnII GATC 6 cut(s) 48, 159, 310, 569, 784, 881
EaeI YGGCCR 2 cut(s) 3, 99
Eam1104I CTCTTC 1 cut(s) 442
EarI CTCTTC 1 cut(s) 442
Ecl136II GAGCTC 1 cut(s) 983
Eco130I CCWWGG 1 cut(s) 903
Eco24I GRGCYC 3 cut(s) 557, 985, 1079
Eco31I GGTCTC 1 cut(s) 950
Eco47I GGWCC 2 cut(s) 653, 926
Eco53kI GAGCTC 1 cut(s) 983
Eco57I CTGAAG 2 cut(s) 336, 765
Eco91I GGTNACC 1 cut(s) 670
EcoICRI GAGCTC 1 cut(s) 983
EcoO109I RGGNCCY 2 cut(s) 554, 926
EcoO65I GGTNACC 1 cut(s) 670
EcoRI GAATTC 2 cut(s) 366, 384
EcoRII CCWGG 1 cut(s) 1104
EcoT14I CCWWGG 1 cut(s) 903
EcoT22I ATGCAT 1 cut(s) 858
EcoT38I GRGCYC 3 cut(s) 557, 985, 1079
ErhI CCWWGG 1 cut(s) 903
FaeI CATG 5 cut(s) 42, 915, 1075, 1084, 1102
FaqI GGGAC 3 cut(s) 666, 683, 1013
FatI CATG 5 cut(s) 38, 911, 1071, 1080, 1098
FauI CCCGC 1 cut(s) 911
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 5 cut(s) 99, 523, 741, 1043, 1057
FokI GGATG 1 cut(s) 747
FriOI GRGCYC 3 cut(s) 557, 985, 1079
Fsp4HI GCNGC 5 cut(s) 99, 523, 741, 1043, 1057
FspBI CTAG 2 cut(s) 422, 717
FspI TGCGCA 1 cut(s) 739
GlaI GCGC 2 cut(s) 217, 739
GluI GCNGC 5 cut(s) 99, 523, 741, 1043, 1057
GsaI CCCAGC 1 cut(s) 653
HaeIII GGCC 5 cut(s) 5, 101, 240, 555, 1103
HapII CCGG 8 cut(s) 222, 308, 357, 381, 675, 681, 702, 759
HgaI GACGC 1 cut(s) 1010
HhaI GCGC 2 cut(s) 218, 740
Hin1I GRCGYC 1 cut(s) 1002
Hin1II CATG 5 cut(s) 42, 915, 1075, 1084, 1102
Hin6I GCGC 2 cut(s) 216, 738
HinP1I GCGC 2 cut(s) 216, 738
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 9 cut(s) 125, 179, 200, 228, 304, 486, 664, 801, 1109
HpaII CCGG 8 cut(s) 222, 308, 357, 381, 675, 681, 702, 759
HphI GGTGA 3 cut(s) 134, 154, 682
Hpy166II GTNNAC 2 cut(s) 538, 894
Hpy188I TCNGA 8 cut(s) 48, 159, 207, 267, 407, 467, 616, 1018
Hpy188III TCNNGA 2 cut(s) 308, 860
Hpy8I GTNNAC 2 cut(s) 538, 894
HpyAV CCTTC 4 cut(s) 318, 567, 1015, 1015
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4IV ACGT 3 cut(s) 469, 658, 825
HpyCH4V TGCA 5 cut(s) 454, 839, 856, 951, 1042
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 98, 104, 222, 347, 719, 989, 1065, 1074
HpyF3I CTNAG 2 cut(s) 1017, 1088
HpySE526I ACGT 3 cut(s) 469, 658, 825
Hsp92I GRCGYC 1 cut(s) 1002
Hsp92II CATG 5 cut(s) 42, 915, 1075, 1084, 1102
HspAI GCGC 2 cut(s) 216, 738
Kpn2I TCCGGA 1 cut(s) 307
KpnI GGTACC 1 cut(s) 681
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 6 cut(s) 48, 159, 310, 569, 784, 881
LmnI GCTCC 2 cut(s) 88, 683
Lsp1109I GCAGC 2 cut(s) 752, 1054
LweI GCATC 2 cut(s) 725, 865
MaeI CTAG 2 cut(s) 422, 717
MaeII ACGT 3 cut(s) 469, 658, 825
MaeIII GTNAC 3 cut(s) 28, 470, 670
MalI GATC 6 cut(s) 50, 161, 312, 571, 786, 883
MbiI CCGCTC 1 cut(s) 566
MboI GATC 6 cut(s) 48, 159, 310, 569, 784, 881
MboII GAAGA 2 cut(s) 333, 459
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 5 cut(s) 172, 557, 974, 985, 1079
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 22, 270, 366, 384, 707, 898, 1062
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 5 cut(s) 173, 313, 480, 795, 1103
MmeI TCCRAC 3 cut(s) 230, 343, 987
MnlI CCTC 7 cut(s) 96, 229, 512, 571, 594, 888, 1097
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 858
MroI TCCGGA 1 cut(s) 307
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 1097
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 743
MspI CCGG 8 cut(s) 222, 308, 357, 381, 675, 681, 702, 759
MspR9I CCNGG 3 cut(s) 358, 702, 1106
Mva1269I GAATGC 2 cut(s) 59, 414
MvaI CCWGG 1 cut(s) 1106
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 9 cut(s) 89, 98, 104, 222, 347, 719, 989, 1065, 1074
NciI CCSGG 2 cut(s) 358, 702
NdeII GATC 6 cut(s) 48, 159, 310, 569, 784, 881
NlaIII CATG 5 cut(s) 42, 915, 1075, 1084, 1102
NlaIV GGNNCC 7 cut(s) 70, 340, 555, 654, 679, 763, 928
NmuCI GTSAC 3 cut(s) 28, 470, 670
NsbI TGCGCA 1 cut(s) 739
NsiI ATGCAT 1 cut(s) 858
NspI RCATGY 2 cut(s) 42, 915
PciI ACATGT 1 cut(s) 38
PctI GAATGC 2 cut(s) 59, 414
PfeI GAWTC 4 cut(s) 125, 200, 228, 664
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 5 cut(s) 100, 524, 742, 1044, 1058
PleI GAGTC 5 cut(s) 173, 312, 480, 795, 1103
PpsI GAGTC 5 cut(s) 173, 312, 480, 795, 1103
Ppu21I YACGTR 1 cut(s) 659
PpuMI RGGWCCY 1 cut(s) 926
PscI ACATGT 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 985
Psp5II RGGWCCY 1 cut(s) 926
Psp6I CCWGG 1 cut(s) 1104
PspEI GGTNACC 1 cut(s) 670
PspFI CCCAGC 1 cut(s) 649
PspGI CCWGG 1 cut(s) 1104
PspN4I GGNNCC 7 cut(s) 70, 340, 555, 654, 679, 763, 928
PspOMI GGGCCC 1 cut(s) 553
PspPI GGNCC 5 cut(s) 553, 554, 653, 926, 1102
PspPPI RGGWCCY 1 cut(s) 926
PsuI RGATCY 1 cut(s) 310
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 5 cut(s) 417, 428, 620, 679, 770
RsaNI GTAC 5 cut(s) 416, 427, 619, 678, 769
RseI CAYNNNNRTG 1 cut(s) 1097
SacI GAGCTC 1 cut(s) 985
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 5 cut(s) 99, 523, 741, 1043, 1057
Sau3AI GATC 6 cut(s) 48, 159, 310, 569, 784, 881
Sau96I GGNCC 5 cut(s) 553, 554, 653, 926, 1102
SchI GAGTC 5 cut(s) 173, 313, 480, 795, 1103
ScrFI CCNGG 3 cut(s) 358, 702, 1106
SduI GDGCHC 5 cut(s) 172, 557, 974, 985, 1079
SfaNI GCATC 2 cut(s) 725, 865
SinI GGWCC 2 cut(s) 653, 926
SmiMI CAYNNNNRTG 1 cut(s) 1097
SmlI CTYRAG 2 cut(s) 87, 301
SmoI CTYRAG 2 cut(s) 87, 301
Sse9I AATT 7 cut(s) 22, 270, 366, 384, 707, 898, 1062
SsiI CCGC 9 cut(s) 98, 214, 251, 287, 522, 564, 776, 918, 1056
SspMI CTAG 2 cut(s) 422, 717
SstI GAGCTC 1 cut(s) 985
StyD4I CCNGG 3 cut(s) 356, 700, 1104
StyI CCWWGG 1 cut(s) 903
TaaI ACNGT 1 cut(s) 517
TaiI ACGT 3 cut(s) 472, 661, 828
TaqI TCGA 3 cut(s) 568, 783, 880
TasI AATT 7 cut(s) 22, 270, 366, 384, 707, 898, 1062
TatI WGTACW 2 cut(s) 415, 768
TauI GCSGC 3 cut(s) 101, 525, 1059
TfiI GAWTC 4 cut(s) 125, 200, 228, 664
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 4 cut(s) 33, 135, 172, 520
TseFI GTSAC 3 cut(s) 28, 470, 670
TseI GCWGC 2 cut(s) 740, 1042
Tsp45I GTSAC 3 cut(s) 28, 470, 670
TspDTI ATGAA 5 cut(s) 650, 820, 856, 1064, 1097
TspRI CASTG 4 cut(s) 33, 135, 172, 520
VpaK11BI GGWCC 2 cut(s) 653, 926
XapI RAATTY 3 cut(s) 22, 366, 384
XceI RCATGY 2 cut(s) 42, 915
XspI CTAG 2 cut(s) 422, 717
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.