Rh3CG014800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0018854
Physical Location & Seq
Reverse (-)
979737 .. 981242
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG014800.1

Sequence Viewer

Length: 1131 bp
ATGATCCAATTAATGGCCTATTCATTATCAAATTCAGTGACGACTATATGTTGTCTGATCTTTTTGGCATTCTTGGGAACCATTTACCTTGCTCCTCAAGCAGAAGCGGCCCGAGCTTTCTTTGTTTTTGGTGATTCACTGGTTGATAATGGCAACAACAACTATCTGATCACCAGTGCTCGTGCCGACTCTTACCCCTACGGCATTGATTCTCCGACACACCGCGCCTCCGGCAGATTCTCCAATGGCCTTAACATTGCGGACATCATCAGTCAGAAAATTGGGTTAGTGGAACCGCCATTGCCATACTTGAGTCCGGATCTAAATGGAAGAAGGCTACTTATTGGTGCCAACTTCGCTTCAGCCGGGGTTGGAATTCTCAATGACACCGGATTTCAGTTTCTAAATATACTCCGAATGCCTATACAACTAGCGTACTTCCAAGAATACCAGAGAAGAGTGCAGGCTCTCATCGGACGTGACCAAACCAAAAGACTCATAGAACAATCCCTCGTCCTTATCACTGTCGGCGGCAATGATTTCGTCAACAACTACTATTTGGGCCCTTCCTCCGCTCGATCTCGCCAACTTCCCCTCCCAAGATATGTCAAGTATGTCATATCCGAGTACGAGAAACTTTTGATGAACTTGTATAAGCTGGGACCACGTAGGATTCTGGTGACCGGTACCGGAGCAATGGGATGTGTCCCCGGAGAATTGGCGACTAGAGCCACGAACGGTGGATGCTCAGCTGAAGTTCAACGAGCCGGTGCCTTGTACAATCCGCAACTCGATCAAATGTTGAAAGGACTCAACAGGAAACTTGGGAAAGACGTTTTCATTTCTGCAAATAATGGACAAATGCATCTGGATTTCATCAACAACCCTCGAGCATTTGGGTTTACTAATTCCAAGGTAGCATGTTGCGGGCAAGGACCCTACAATGGAGTCGGTCTCTGCACATTTCTGTCCAACTTGTGCCCTAACAGAGCTCTCTATGCGTTTTGGGACGCCTTCCACCCATCTGAGAAGGCTAATAGAATTATTGTGCAGAAGATGAATAGCGGCTCAACTCGCTACATGAGCCCCATGAACCTCAGCACAATCATGGCCCTGGATTCTACTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

376

Amino Acids

41.31

Weight (kDa)

9.29

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 351 9.4e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018854)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01370
malus_domestica MD04G1234800.v1.1
rosa_chinensis RchiOBHm_Chr3g0448941
rosa_laevigata RLG00000025864
rosa_multiflora Rmu_sc0003116.1_g000015
rosa_roxburghii Rroxscaffold_6G00427120
rosa_rugosa Rorug02G0617200
rosa_samantha Rh3CG014800
rosa_wichuraiana Rw3G001170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 686
AccB1I GGYRCC 3 cut(s) 347, 686, 770
AccB7I CCANNNNNTGG 1 cut(s) 13
AccBSI CCGCTC 1 cut(s) 575
AccII CGCG 1 cut(s) 225
AccIII TCCGGA 1 cut(s) 316
AciI CCGC 9 cut(s) 107, 223, 260, 296, 531, 573, 785, 927, 1065
AclWI GGATC 1 cut(s) 327
AcsI RAATTY 2 cut(s) 31, 375
AcuI CTGAAG 2 cut(s) 345, 774
AcyI GRCGYC 1 cut(s) 1011
AfaI GTAC 4 cut(s) 437, 629, 688, 779
AfiI CCNNNNNNNGG 2 cut(s) 13, 944
AgeI ACCGGT 1 cut(s) 683
AgsI TTSAA 2 cut(s) 761, 805
AjiI CACGTC 1 cut(s) 479
AjnI CCWGG 1 cut(s) 1113
AluBI AGCT 4 cut(s) 116, 658, 752, 992
AluI AGCT 4 cut(s) 116, 658, 752, 992
Alw21I GWGCWC 2 cut(s) 181, 994
Alw26I GTCTC 1 cut(s) 959
AlwI GGATC 1 cut(s) 327
Ama87I CYCGRG 2 cut(s) 111, 888
Aor13HI TCCGGA 1 cut(s) 316
AoxI GGCC 5 cut(s) 15, 108, 247, 562, 1110
ApaI GGGCCC 1 cut(s) 566
ApoI RAATTY 2 cut(s) 31, 375
AseI ATTAAT 1 cut(s) 11
AsiGI ACCGGT 1 cut(s) 683
Asp718I GGTACC 1 cut(s) 686
AspLEI GCGC 1 cut(s) 227
AspS9I GGNCC 6 cut(s) 109, 562, 563, 662, 935, 1111
AsuC2I CCSGG 2 cut(s) 367, 711
AsuHPI GGTGA 3 cut(s) 143, 163, 691
AvaI CYCGRG 2 cut(s) 111, 888
AvaII GGWCC 2 cut(s) 662, 935
BaeGI GKGCMC 2 cut(s) 566, 983
BanI GGYRCC 3 cut(s) 347, 686, 770
BanII GRGCYC 3 cut(s) 566, 994, 1088
BauI CACGAG 1 cut(s) 180
Bbv12I GWGCWC 2 cut(s) 181, 994
BbvCI CCTCAGC 1 cut(s) 1097
BccI CCATC 1 cut(s) 1030
BceAI ACGGC 1 cut(s) 217
BcgI CGANNNNNNTGC 2 cut(s) 523, 557
BciT130I CCWGG 1 cut(s) 1115
BclI TGATCA 1 cut(s) 168
BcnI CCSGG 2 cut(s) 367, 711
BcoDI GTCTC 1 cut(s) 959
BfaI CTAG 2 cut(s) 431, 726
BisI GCNGC 3 cut(s) 108, 532, 1066
BlpI GCTNAGC 1 cut(s) 748
BlsI GCNGC 3 cut(s) 109, 533, 1067
Bme1390I CCNGG 3 cut(s) 367, 711, 1115
Bme18I GGWCC 2 cut(s) 662, 935
BmeT110I CYCGRG 2 cut(s) 111, 888
BmgBI CACGTC 1 cut(s) 479
BmgT120I GGNCC 6 cut(s) 109, 562, 563, 662, 935, 1111
BmiI GGNNCC 8 cut(s) 79, 294, 349, 564, 663, 688, 772, 937
BmrFI CCNGG 3 cut(s) 367, 711, 1115
BmsI GCATC 2 cut(s) 734, 874
BplI GAGNNNNNCTC 2 cut(s) 939, 971
Bpu10I CCTNAGC 1 cut(s) 1097
Bpu1102I GCTNAGC 1 cut(s) 748
BpuEI CTTGAG 2 cut(s) 81, 331
BpuMI CCSGG 2 cut(s) 367, 711
BsaAI YACGTR 1 cut(s) 668
BsaHI GRCGYC 1 cut(s) 1011
BsaI GGTCTC 1 cut(s) 959
BsaJI CCNNGG 4 cut(s) 366, 709, 912, 1113
BsaWI WCCGGW 4 cut(s) 316, 389, 683, 689
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 579, 609
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 944
Bse118I RCCGGY 2 cut(s) 683, 767
Bse1I ACTGG 2 cut(s) 144, 174
Bse3DI GCAATG 4 cut(s) 255, 299, 541, 702
BseAI TCCGGA 1 cut(s) 316
BseBI CCWGG 1 cut(s) 1115
BseDI CCNNGG 4 cut(s) 366, 709, 912, 1113
BseGI GGATG 2 cut(s) 707, 749
BseLI CCNNNNNNNGG 2 cut(s) 13, 944
BseMI GCAATG 4 cut(s) 255, 299, 541, 702
BseMII CTCAG 3 cut(s) 762, 1017, 1111
BseNI ACTGG 2 cut(s) 144, 174
BseRI GAGGAG 1 cut(s) 84
BseSI GKGCMC 2 cut(s) 566, 983
BseYI CCCAGC 1 cut(s) 658
BsgI GTGCAG 3 cut(s) 482, 943, 1070
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 5 cut(s) 17, 110, 249, 564, 1112
BshNI GGYRCC 3 cut(s) 347, 686, 770
BshTI ACCGGT 1 cut(s) 683
BsiHKAI GWGCWC 2 cut(s) 181, 994
BsiHKCI CYCGRG 2 cut(s) 111, 888
BsiSI CCGG 8 cut(s) 231, 317, 366, 390, 684, 690, 711, 768
BslFI GGGAC 3 cut(s) 675, 692, 1022
BslI CCNNNNNNNGG 2 cut(s) 13, 944
BsmAI GTCTC 1 cut(s) 959
BsmFI GGGAC 3 cut(s) 675, 692, 1022
BsmI GAATGC 2 cut(s) 68, 423
BsnI GGCC 5 cut(s) 17, 110, 249, 564, 1112
Bso31I GGTCTC 1 cut(s) 959
BsoBI CYCGRG 2 cut(s) 111, 888
Bsp120I GGGCCC 1 cut(s) 562
Bsp1286I GDGCHC 5 cut(s) 181, 566, 983, 994, 1088
Bsp13I TCCGGA 1 cut(s) 316
Bsp1407I TGTACA 1 cut(s) 777
Bsp143I GATC 6 cut(s) 3, 57, 168, 319, 578, 793
Bsp1720I GCTNAGC 1 cut(s) 748
BspACI CCGC 9 cut(s) 107, 223, 260, 296, 531, 573, 785, 927, 1065
BspANI GGCC 5 cut(s) 17, 110, 249, 564, 1112
BspCNI CTCAG 3 cut(s) 761, 1018, 1110
BspEI TCCGGA 1 cut(s) 316
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 8 cut(s) 79, 294, 349, 564, 663, 688, 772, 937
BspPI GGATC 1 cut(s) 327
BspT107I GGYRCC 3 cut(s) 347, 686, 770
BspTNI GGTCTC 1 cut(s) 959
BsrBI CCGCTC 1 cut(s) 575
BsrDI GCAATG 4 cut(s) 255, 299, 541, 702
BsrFI RCCGGY 2 cut(s) 683, 767
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 2 cut(s) 144, 174
BssAI RCCGGY 2 cut(s) 683, 767
BssECI CCNNGG 4 cut(s) 366, 709, 912, 1113
BssMI GATC 6 cut(s) 3, 57, 168, 319, 578, 793
BssNI GRCGYC 1 cut(s) 1011
BssSI CACGAG 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 912
Bst2BI CACGAG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 1115
Bst4CI ACNGT 2 cut(s) 526, 740
Bst6I CTCTTC 1 cut(s) 451
BstACI GRCGYC 1 cut(s) 1011
BstAUI TGTACA 1 cut(s) 777
BstBAI YACGTR 1 cut(s) 668
BstC8I GCNNGC 2 cut(s) 465, 929
BstDEI CTNAG 3 cut(s) 748, 1026, 1097
BstEII GGTNACC 1 cut(s) 679
BstF5I GGATG 2 cut(s) 707, 749
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 6 cut(s) 6, 60, 171, 322, 581, 796
BstMAI GTCTC 1 cut(s) 959
BstMBI GATC 6 cut(s) 3, 57, 168, 319, 578, 793
BstMWI GCNNNNNNNGC 9 cut(s) 98, 107, 113, 231, 356, 728, 998, 1074, 1083
BstNI CCWGG 1 cut(s) 1115
BstNSI RCATGY 1 cut(s) 924
BstPI GGTNACC 1 cut(s) 679
BstSCI CCNGG 3 cut(s) 365, 709, 1113
BstSLI GKGCMC 2 cut(s) 566, 983
BstUI CGCG 1 cut(s) 225
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BsuRI GGCC 5 cut(s) 17, 110, 249, 564, 1112
BtrI CACGTC 1 cut(s) 479
BtsCI GGATG 2 cut(s) 707, 749
BtsIMutI CAGTG 4 cut(s) 42, 137, 181, 522
Cac8I GCNNGC 2 cut(s) 465, 929
CfoI GCGC 1 cut(s) 227
Cfr10I RCCGGY 2 cut(s) 683, 767
Cfr13I GGNCC 6 cut(s) 109, 562, 563, 662, 935, 1111
CseI GACGC 1 cut(s) 1019
Csp6I GTAC 4 cut(s) 436, 628, 687, 778
CspAI ACCGGT 1 cut(s) 683
CviAII CATG 4 cut(s) 921, 1081, 1090, 1108
CviQI GTAC 4 cut(s) 436, 628, 687, 778
DdeI CTNAG 3 cut(s) 748, 1026, 1097
DpnI GATC 6 cut(s) 5, 59, 170, 321, 580, 795
DpnII GATC 6 cut(s) 3, 57, 168, 319, 578, 793
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
Ecl136II GAGCTC 1 cut(s) 992
Eco130I CCWWGG 1 cut(s) 912
Eco24I GRGCYC 3 cut(s) 566, 994, 1088
Eco31I GGTCTC 1 cut(s) 959
Eco47I GGWCC 2 cut(s) 662, 935
Eco53kI GAGCTC 1 cut(s) 992
Eco57I CTGAAG 2 cut(s) 345, 774
Eco88I CYCGRG 2 cut(s) 111, 888
Eco91I GGTNACC 1 cut(s) 679
EcoICRI GAGCTC 1 cut(s) 992
EcoO109I RGGNCCY 2 cut(s) 563, 935
EcoO65I GGTNACC 1 cut(s) 679
EcoRI GAATTC 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 1113
EcoT14I CCWWGG 1 cut(s) 912
EcoT22I ATGCAT 1 cut(s) 867
EcoT38I GRGCYC 3 cut(s) 566, 994, 1088
ErhI CCWWGG 1 cut(s) 912
FaeI CATG 4 cut(s) 924, 1084, 1093, 1111
FaqI GGGAC 3 cut(s) 675, 692, 1022
FatI CATG 4 cut(s) 920, 1080, 1089, 1107
FauI CCCGC 1 cut(s) 920
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 108, 532, 1066
FokI GGATG 2 cut(s) 714, 756
FriOI GRGCYC 3 cut(s) 566, 994, 1088
Fsp4HI GCNGC 3 cut(s) 108, 532, 1066
FspBI CTAG 2 cut(s) 431, 726
GlaI GCGC 1 cut(s) 226
GluI GCNGC 3 cut(s) 108, 532, 1066
GsaI CCCAGC 1 cut(s) 662
HaeIII GGCC 5 cut(s) 17, 110, 249, 564, 1112
HapII CCGG 8 cut(s) 231, 317, 366, 390, 684, 690, 711, 768
HgaI GACGC 1 cut(s) 1019
HhaI GCGC 1 cut(s) 227
Hin1I GRCGYC 1 cut(s) 1011
Hin1II CATG 4 cut(s) 924, 1084, 1093, 1111
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HpaII CCGG 8 cut(s) 231, 317, 366, 390, 684, 690, 711, 768
HphI GGTGA 3 cut(s) 143, 163, 691
Hpy166II GTNNAC 2 cut(s) 547, 903
Hpy188I TCNGA 8 cut(s) 57, 168, 216, 276, 416, 476, 625, 1027
Hpy188III TCNNGA 2 cut(s) 317, 869
Hpy8I GTNNAC 2 cut(s) 547, 903
HpyAV CCTTC 4 cut(s) 327, 576, 1024, 1024
HpyCH4III ACNGT 2 cut(s) 526, 740
HpyCH4IV ACGT 3 cut(s) 478, 667, 834
HpyCH4V TGCA 5 cut(s) 463, 848, 865, 960, 1051
HpyF10VI GCNNNNNNNGC 9 cut(s) 98, 107, 113, 231, 356, 728, 998, 1074, 1083
HpyF3I CTNAG 3 cut(s) 748, 1026, 1097
HpySE526I ACGT 3 cut(s) 478, 667, 834
Hsp92I GRCGYC 1 cut(s) 1011
Hsp92II CATG 4 cut(s) 924, 1084, 1093, 1111
HspAI GCGC 1 cut(s) 225
Kpn2I TCCGGA 1 cut(s) 316
KpnI GGTACC 1 cut(s) 690
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 6 cut(s) 3, 57, 168, 319, 578, 793
LmnI GCTCC 2 cut(s) 97, 692
LweI GCATC 2 cut(s) 734, 874
MaeI CTAG 2 cut(s) 431, 726
MaeII ACGT 3 cut(s) 478, 667, 834
MaeIII GTNAC 3 cut(s) 37, 479, 679
MalI GATC 6 cut(s) 5, 59, 170, 321, 580, 795
MbiI CCGCTC 1 cut(s) 575
MboI GATC 6 cut(s) 3, 57, 168, 319, 578, 793
MboII GAAGA 3 cut(s) 342, 468, 1066
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 5 cut(s) 181, 566, 983, 994, 1088
MluCI AATT 7 cut(s) 8, 31, 279, 375, 716, 907, 1041
MlyI GAGTC 5 cut(s) 182, 322, 489, 804, 957
MmeI TCCRAC 3 cut(s) 239, 352, 996
MnlI CCTC 7 cut(s) 105, 238, 521, 580, 605, 897, 1106
Mph1103I ATGCAT 1 cut(s) 867
MroI TCCGGA 1 cut(s) 316
MseI TTAA 2 cut(s) 11, 252
MslI CAYNNNNRTG 1 cut(s) 1106
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 8 cut(s) 231, 317, 366, 390, 684, 690, 711, 768
MspR9I CCNGG 3 cut(s) 367, 711, 1115
Mva1269I GAATGC 2 cut(s) 68, 423
MvaI CCWGG 1 cut(s) 1115
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 9 cut(s) 98, 107, 113, 231, 356, 728, 998, 1074, 1083
NciI CCSGG 2 cut(s) 367, 711
NdeII GATC 6 cut(s) 3, 57, 168, 319, 578, 793
NlaIII CATG 4 cut(s) 924, 1084, 1093, 1111
NlaIV GGNNCC 8 cut(s) 79, 294, 349, 564, 663, 688, 772, 937
NmuCI GTSAC 3 cut(s) 37, 479, 679
NsiI ATGCAT 1 cut(s) 867
NspI RCATGY 1 cut(s) 924
PaeR7I CTCGAG 1 cut(s) 888
PctI GAATGC 2 cut(s) 68, 423
PfeI GAWTC 5 cut(s) 134, 209, 237, 673, 1118
PflMI CCANNNNNTGG 1 cut(s) 13
PinAI ACCGGT 1 cut(s) 683
PkrI GCNGC 3 cut(s) 109, 533, 1067
PleI GAGTC 5 cut(s) 182, 321, 489, 804, 956
PpsI GAGTC 5 cut(s) 182, 321, 489, 804, 956
Ppu21I YACGTR 1 cut(s) 668
PpuMI RGGWCCY 1 cut(s) 935
PshBI ATTAAT 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 994
Psp5II RGGWCCY 1 cut(s) 935
Psp6I CCWGG 1 cut(s) 1113
PspEI GGTNACC 1 cut(s) 679
PspFI CCCAGC 1 cut(s) 658
PspGI CCWGG 1 cut(s) 1113
PspN4I GGNNCC 8 cut(s) 79, 294, 349, 564, 663, 688, 772, 937
PspOMI GGGCCC 1 cut(s) 562
PspPI GGNCC 6 cut(s) 109, 562, 563, 662, 935, 1111
PspPPI RGGWCCY 1 cut(s) 935
PspXI VCTCGAGB 1 cut(s) 888
PsuI RGATCY 1 cut(s) 319
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 4 cut(s) 437, 629, 688, 779
RsaNI GTAC 4 cut(s) 436, 628, 687, 778
RseI CAYNNNNRTG 1 cut(s) 1106
SacI GAGCTC 1 cut(s) 994
SaqAI TTAA 2 cut(s) 11, 252
SatI GCNGC 3 cut(s) 108, 532, 1066
Sau3AI GATC 6 cut(s) 3, 57, 168, 319, 578, 793
Sau96I GGNCC 6 cut(s) 109, 562, 563, 662, 935, 1111
SchI GAGTC 5 cut(s) 182, 322, 489, 804, 957
ScrFI CCNGG 3 cut(s) 367, 711, 1115
SduI GDGCHC 5 cut(s) 181, 566, 983, 994, 1088
SfaNI GCATC 2 cut(s) 734, 874
Sfr274I CTCGAG 1 cut(s) 888
SinI GGWCC 2 cut(s) 662, 935
SlaI CTCGAG 1 cut(s) 888
SmiMI CAYNNNNRTG 1 cut(s) 1106
SmlI CTYRAG 3 cut(s) 96, 310, 888
SmoI CTYRAG 3 cut(s) 96, 310, 888
Sse9I AATT 7 cut(s) 8, 31, 279, 375, 716, 907, 1041
SsiI CCGC 9 cut(s) 107, 223, 260, 296, 531, 573, 785, 927, 1065
SspMI CTAG 2 cut(s) 431, 726
SstI GAGCTC 1 cut(s) 994
StyD4I CCNGG 3 cut(s) 365, 709, 1113
StyI CCWWGG 1 cut(s) 912
TaaI ACNGT 2 cut(s) 526, 740
TaiI ACGT 3 cut(s) 481, 670, 837
TaqI TCGA 3 cut(s) 577, 792, 889
TaqII GACCGA 1 cut(s) 941
TasI AATT 7 cut(s) 8, 31, 279, 375, 716, 907, 1041
TatI WGTACW 1 cut(s) 777
TauI GCSGC 3 cut(s) 110, 534, 1068
TfiI GAWTC 5 cut(s) 134, 209, 237, 673, 1118
Tru1I TTAA 2 cut(s) 11, 252
Tru9I TTAA 2 cut(s) 11, 252
TscAI CASTG 4 cut(s) 42, 144, 181, 529
TseFI GTSAC 3 cut(s) 37, 479, 679
Tsp45I GTSAC 3 cut(s) 37, 479, 679
TspDTI ATGAA 6 cut(s) 12, 659, 829, 865, 1073, 1106
TspRI CASTG 4 cut(s) 42, 144, 181, 529
Van91I CCANNNNNTGG 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 662, 935
VspI ATTAAT 1 cut(s) 11
XapI RAATTY 2 cut(s) 31, 375
XceI RCATGY 1 cut(s) 924
XhoI CTCGAG 1 cut(s) 888
XspI CTAG 2 cut(s) 431, 726
Zsp2I ATGCAT 1 cut(s) 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.