RchiOBHm_Chr3g0448941

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0018854
Physical Location & Seq
Reverse (-)
953476 .. 955174
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41636

Sequence Viewer

Length: 1134 bp
ATGATCCAATTAATGGCCACCTATTCATTATCAAATTCAGTGACGACTATATGTTGTCTGATCTTTTTGCCATTCTTGGGAACCATTTACCTTGCTCCTCAAGCAGAAGCGGCCCGAGCTTTCTTTGTTTTTGGTGATTCACTGGTTGATAATGGCAACAACAACTATCTGATCACCAGTGCTCGTGCCGACTCTTACCCCTACGGCATTGATTCTCCGACACACCGCCCCTCCGGCAGATTCTCCAATGGCCTTAACATTGCGGACATCATCAGTCAGAAAATTGGGTCAGTGGAACCGCCATTGCCATACTTGAGTCCGGATCTAAATGGAAGAAGGCTACTTATTGGTGCCAACTTCGCTTCAGCCGGGGTTGGAATTCTCAATGACACCGGAATTCAGTTTCTAAATATACTCCGAATGCCTATACAACTAGCGTACTTCCAAGAATACCAGAGAAGAGTGCAGGCTCTCATCGGACGTGACCAAGCCAAAAGACTCATAGAACAATCCCTCGTCCTTATCACTGTCGGCGGCAATGATTTCGTCAACAACTACTATTTGGGCCCTTCCTCCGCTCGATCTCGCCAACTTCCCCTCCCAAGATATGTCAAGTATGTCATATCCGAGTACGAGAAACTTTTGATGAACTTGTATAAGCTAGGACCACGTAGGATTCTGGTGACTGGTACCGGAGCAATGGGATGTGTCCCCGGAGAATTGGCGACTAGAGCCACGAACGGTGGATGCTCAGCTGAAGTTCAACGAGCCGGTGCCTTGTACAATCCGCAACTCGATCAAATGTTGAAAGGACTCAACAAGAAACTTGGGAAAGACGTTTTCATTTCTGCAAATAATGGACAAATGCATCTGGATTTCATCAACAACCCTCGAGCATTTGGGTTTACTAATTCCAAGGTTGCATGTTGCGGGCAAGGACCCTACAATGGAGTCGGTCTCTGCACATTTCTGTCCAACTTGTGCCCCAACAGAGCTCTCTATGCGTTTTGGGACGCCTTCCACCCATCTGAGAAGGCTAATAGAATTATTGTGCAGAAGATGAATAGCGGCTCAATTCGCTACATGAGCCCCATGAACCTCAGCACAATCATGGCCCTGGACTCTACTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

377

Amino Acids

41.36

Weight (kDa)

9.27

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 352 1.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018854)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01370
malus_domestica MD04G1234800.v1.1
rosa_chinensis RchiOBHm_Chr3g0448941
rosa_laevigata RLG00000025864
rosa_multiflora Rmu_sc0003116.1_g000015
rosa_roxburghii Rroxscaffold_6G00427120
rosa_rugosa Rorug02G0617200
rosa_samantha Rh3CG014800
rosa_wichuraiana Rw3G001170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 689
AccB1I GGYRCC 3 cut(s) 350, 689, 773
AccB7I CCANNNNNTGG 1 cut(s) 13
AccBSI CCGCTC 1 cut(s) 578
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 9 cut(s) 110, 226, 263, 299, 534, 576, 788, 930, 1068
AclWI GGATC 1 cut(s) 330
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 3 cut(s) 34, 378, 396
AcuI CTGAAG 2 cut(s) 348, 777
AcyI GRCGYC 1 cut(s) 1014
AfaI GTAC 4 cut(s) 440, 632, 691, 782
AfiI CCNNNNNNNGG 3 cut(s) 13, 77, 947
AgsI TTSAA 2 cut(s) 764, 808
AjiI CACGTC 1 cut(s) 482
AjnI CCWGG 1 cut(s) 1116
AluBI AGCT 4 cut(s) 119, 661, 755, 995
AluI AGCT 4 cut(s) 119, 661, 755, 995
Alw21I GWGCWC 2 cut(s) 184, 997
Alw26I GTCTC 1 cut(s) 962
AlwI GGATC 1 cut(s) 330
Ama87I CYCGRG 2 cut(s) 114, 891
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 5 cut(s) 15, 111, 250, 565, 1113
ApaI GGGCCC 1 cut(s) 569
ApoI RAATTY 3 cut(s) 34, 378, 396
AseI ATTAAT 1 cut(s) 11
Asp718I GGTACC 1 cut(s) 689
AspS9I GGNCC 6 cut(s) 112, 565, 566, 665, 938, 1114
AsuC2I CCSGG 2 cut(s) 370, 714
AsuHPI GGTGA 3 cut(s) 146, 166, 694
AvaI CYCGRG 2 cut(s) 114, 891
AvaII GGWCC 2 cut(s) 665, 938
BaeGI GKGCMC 2 cut(s) 569, 986
BalI TGGCCA 1 cut(s) 17
BanI GGYRCC 3 cut(s) 350, 689, 773
BanII GRGCYC 3 cut(s) 569, 997, 1091
BauI CACGAG 1 cut(s) 183
Bbv12I GWGCWC 2 cut(s) 184, 997
BbvCI CCTCAGC 1 cut(s) 1100
BccI CCATC 1 cut(s) 1033
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 526, 560
BciT130I CCWGG 1 cut(s) 1118
BclI TGATCA 1 cut(s) 171
BcnI CCSGG 2 cut(s) 370, 714
BcoDI GTCTC 1 cut(s) 962
BfaI CTAG 3 cut(s) 434, 662, 729
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 3 cut(s) 111, 535, 1069
BlpI GCTNAGC 1 cut(s) 751
BlsI GCNGC 3 cut(s) 112, 536, 1070
Bme1390I CCNGG 3 cut(s) 370, 714, 1118
Bme18I GGWCC 2 cut(s) 665, 938
BmeT110I CYCGRG 2 cut(s) 114, 891
BmgBI CACGTC 1 cut(s) 482
BmgT120I GGNCC 6 cut(s) 112, 565, 566, 665, 938, 1114
BmiI GGNNCC 7 cut(s) 82, 297, 352, 567, 691, 775, 940
BmrFI CCNGG 3 cut(s) 370, 714, 1118
BmsI GCATC 2 cut(s) 737, 877
BplI GAGNNNNNCTC 2 cut(s) 942, 974
Bpu10I CCTNAGC 1 cut(s) 1100
Bpu1102I GCTNAGC 1 cut(s) 751
BpuEI CTTGAG 2 cut(s) 84, 334
BpuMI CCSGG 2 cut(s) 370, 714
BsaAI YACGTR 1 cut(s) 671
BsaHI GRCGYC 1 cut(s) 1014
BsaI GGTCTC 1 cut(s) 962
BsaJI CCNNGG 4 cut(s) 369, 712, 915, 1116
BsaWI WCCGGW 3 cut(s) 319, 392, 692
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 582, 612
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 77, 947
Bse118I RCCGGY 1 cut(s) 770
Bse1I ACTGG 3 cut(s) 147, 177, 691
Bse3DI GCAATG 4 cut(s) 258, 302, 544, 705
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 1 cut(s) 1118
BseDI CCNNGG 4 cut(s) 369, 712, 915, 1116
BseGI GGATG 2 cut(s) 710, 752
BseLI CCNNNNNNNGG 3 cut(s) 13, 77, 947
BseMI GCAATG 4 cut(s) 258, 302, 544, 705
BseMII CTCAG 3 cut(s) 765, 1020, 1114
BseNI ACTGG 3 cut(s) 147, 177, 691
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 2 cut(s) 569, 986
BsgI GTGCAG 3 cut(s) 485, 946, 1073
BshFI GGCC 5 cut(s) 17, 113, 252, 567, 1115
BshNI GGYRCC 3 cut(s) 350, 689, 773
BsiHKAI GWGCWC 2 cut(s) 184, 997
BsiHKCI CYCGRG 2 cut(s) 114, 891
BsiSI CCGG 7 cut(s) 234, 320, 369, 393, 693, 714, 771
BslFI GGGAC 2 cut(s) 695, 1025
BslI CCNNNNNNNGG 3 cut(s) 13, 77, 947
BsmAI GTCTC 1 cut(s) 962
BsmFI GGGAC 2 cut(s) 695, 1025
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 5 cut(s) 17, 113, 252, 567, 1115
Bso31I GGTCTC 1 cut(s) 962
BsoBI CYCGRG 2 cut(s) 114, 891
Bsp120I GGGCCC 1 cut(s) 565
Bsp1286I GDGCHC 5 cut(s) 184, 569, 986, 997, 1091
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 780
Bsp143I GATC 6 cut(s) 3, 60, 171, 322, 581, 796
Bsp1720I GCTNAGC 1 cut(s) 751
BspACI CCGC 9 cut(s) 110, 226, 263, 299, 534, 576, 788, 930, 1068
BspANI GGCC 5 cut(s) 17, 113, 252, 567, 1115
BspCNI CTCAG 3 cut(s) 764, 1021, 1113
BspEI TCCGGA 1 cut(s) 319
BspLI GGNNCC 7 cut(s) 82, 297, 352, 567, 691, 775, 940
BspPI GGATC 1 cut(s) 330
BspT107I GGYRCC 3 cut(s) 350, 689, 773
BspTNI GGTCTC 1 cut(s) 962
BsrBI CCGCTC 1 cut(s) 578
BsrDI GCAATG 4 cut(s) 258, 302, 544, 705
BsrFI RCCGGY 1 cut(s) 770
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 3 cut(s) 147, 177, 691
BssAI RCCGGY 1 cut(s) 770
BssECI CCNNGG 4 cut(s) 369, 712, 915, 1116
BssMI GATC 6 cut(s) 3, 60, 171, 322, 581, 796
BssNI GRCGYC 1 cut(s) 1014
BssSI CACGAG 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 915
Bst2BI CACGAG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 1118
Bst4CI ACNGT 2 cut(s) 529, 743
Bst6I CTCTTC 1 cut(s) 454
BstACI GRCGYC 1 cut(s) 1014
BstAUI TGTACA 1 cut(s) 780
BstBAI YACGTR 1 cut(s) 671
BstC8I GCNNGC 2 cut(s) 468, 932
BstDEI CTNAG 3 cut(s) 751, 1029, 1100
BstF5I GGATG 2 cut(s) 710, 752
BstKTI GATC 6 cut(s) 6, 63, 174, 325, 584, 799
BstMAI GTCTC 1 cut(s) 962
BstMBI GATC 6 cut(s) 3, 60, 171, 322, 581, 796
BstMWI GCNNNNNNNGC 9 cut(s) 101, 110, 116, 234, 359, 731, 1001, 1077, 1086
BstNI CCWGG 1 cut(s) 1118
BstNSI RCATGY 1 cut(s) 927
BstSCI CCNGG 3 cut(s) 368, 712, 1116
BstSLI GKGCMC 2 cut(s) 569, 986
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BsuRI GGCC 5 cut(s) 17, 113, 252, 567, 1115
BtrI CACGTC 1 cut(s) 482
BtsCI GGATG 2 cut(s) 710, 752
BtsIMutI CAGTG 5 cut(s) 45, 140, 184, 297, 525
Cac8I GCNNGC 2 cut(s) 468, 932
Cfr10I RCCGGY 1 cut(s) 770
Cfr13I GGNCC 6 cut(s) 112, 565, 566, 665, 938, 1114
CseI GACGC 1 cut(s) 1022
Csp6I GTAC 4 cut(s) 439, 631, 690, 781
CviAII CATG 4 cut(s) 924, 1084, 1093, 1111
CviQI GTAC 4 cut(s) 439, 631, 690, 781
DdeI CTNAG 3 cut(s) 751, 1029, 1100
DpnI GATC 6 cut(s) 5, 62, 173, 324, 583, 798
DpnII GATC 6 cut(s) 3, 60, 171, 322, 581, 796
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 454
Ecl136II GAGCTC 1 cut(s) 995
Eco130I CCWWGG 1 cut(s) 915
Eco24I GRGCYC 3 cut(s) 569, 997, 1091
Eco31I GGTCTC 1 cut(s) 962
Eco47I GGWCC 2 cut(s) 665, 938
Eco53kI GAGCTC 1 cut(s) 995
Eco57I CTGAAG 2 cut(s) 348, 777
Eco88I CYCGRG 2 cut(s) 114, 891
EcoICRI GAGCTC 1 cut(s) 995
EcoO109I RGGNCCY 2 cut(s) 566, 938
EcoRI GAATTC 2 cut(s) 378, 396
EcoRII CCWGG 1 cut(s) 1116
EcoT14I CCWWGG 1 cut(s) 915
EcoT22I ATGCAT 1 cut(s) 870
EcoT38I GRGCYC 3 cut(s) 569, 997, 1091
ErhI CCWWGG 1 cut(s) 915
FaeI CATG 4 cut(s) 927, 1087, 1096, 1114
FaqI GGGAC 2 cut(s) 695, 1025
FatI CATG 4 cut(s) 923, 1083, 1092, 1110
FauI CCCGC 1 cut(s) 923
FbaI TGATCA 1 cut(s) 171
Fnu4HI GCNGC 3 cut(s) 111, 535, 1069
FokI GGATG 2 cut(s) 717, 759
FriOI GRGCYC 3 cut(s) 569, 997, 1091
Fsp4HI GCNGC 3 cut(s) 111, 535, 1069
FspBI CTAG 3 cut(s) 434, 662, 729
GluI GCNGC 3 cut(s) 111, 535, 1069
HaeIII GGCC 5 cut(s) 17, 113, 252, 567, 1115
HapII CCGG 7 cut(s) 234, 320, 369, 393, 693, 714, 771
HgaI GACGC 1 cut(s) 1022
Hin1I GRCGYC 1 cut(s) 1014
Hin1II CATG 4 cut(s) 927, 1087, 1096, 1114
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HpaII CCGG 7 cut(s) 234, 320, 369, 393, 693, 714, 771
HphI GGTGA 3 cut(s) 146, 166, 694
Hpy166II GTNNAC 2 cut(s) 550, 906
Hpy188I TCNGA 8 cut(s) 60, 171, 219, 279, 419, 479, 628, 1030
Hpy188III TCNNGA 2 cut(s) 320, 872
Hpy8I GTNNAC 2 cut(s) 550, 906
HpyAV CCTTC 4 cut(s) 330, 579, 1027, 1027
HpyCH4III ACNGT 2 cut(s) 529, 743
HpyCH4IV ACGT 3 cut(s) 481, 670, 837
HpyCH4V TGCA 6 cut(s) 466, 851, 868, 923, 963, 1054
HpyF10VI GCNNNNNNNGC 9 cut(s) 101, 110, 116, 234, 359, 731, 1001, 1077, 1086
HpyF3I CTNAG 3 cut(s) 751, 1029, 1100
HpySE526I ACGT 3 cut(s) 481, 670, 837
Hsp92I GRCGYC 1 cut(s) 1014
Hsp92II CATG 4 cut(s) 927, 1087, 1096, 1114
Kpn2I TCCGGA 1 cut(s) 319
KpnI GGTACC 1 cut(s) 693
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 6 cut(s) 3, 60, 171, 322, 581, 796
LmnI GCTCC 2 cut(s) 100, 695
LweI GCATC 2 cut(s) 737, 877
MaeI CTAG 3 cut(s) 434, 662, 729
MaeII ACGT 3 cut(s) 481, 670, 837
MaeIII GTNAC 3 cut(s) 40, 482, 682
MalI GATC 6 cut(s) 5, 62, 173, 324, 583, 798
MbiI CCGCTC 1 cut(s) 578
MboI GATC 6 cut(s) 3, 60, 171, 322, 581, 796
MboII GAAGA 3 cut(s) 345, 471, 1069
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 5 cut(s) 184, 569, 986, 997, 1091
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 9 cut(s) 8, 34, 282, 378, 396, 719, 910, 1044, 1074
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 6 cut(s) 185, 325, 492, 807, 960, 1115
MmeI TCCRAC 3 cut(s) 242, 355, 999
MnlI CCTC 7 cut(s) 108, 241, 524, 583, 608, 900, 1109
Mox20I TGGCCA 1 cut(s) 17
Mph1103I ATGCAT 1 cut(s) 870
MroI TCCGGA 1 cut(s) 319
MscI TGGCCA 1 cut(s) 17
MseI TTAA 2 cut(s) 11, 255
MslI CAYNNNNRTG 1 cut(s) 1109
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 755
MspI CCGG 7 cut(s) 234, 320, 369, 393, 693, 714, 771
MspR9I CCNGG 3 cut(s) 370, 714, 1118
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 1 cut(s) 1118
MwoI GCNNNNNNNGC 9 cut(s) 101, 110, 116, 234, 359, 731, 1001, 1077, 1086
NciI CCSGG 2 cut(s) 370, 714
NdeII GATC 6 cut(s) 3, 60, 171, 322, 581, 796
NlaIII CATG 4 cut(s) 927, 1087, 1096, 1114
NlaIV GGNNCC 7 cut(s) 82, 297, 352, 567, 691, 775, 940
NmuCI GTSAC 3 cut(s) 40, 482, 682
NsiI ATGCAT 1 cut(s) 870
NspI RCATGY 1 cut(s) 927
PaeR7I CTCGAG 1 cut(s) 891
PctI GAATGC 1 cut(s) 426
PfeI GAWTC 4 cut(s) 137, 212, 240, 676
PflMI CCANNNNNTGG 1 cut(s) 13
PkrI GCNGC 3 cut(s) 112, 536, 1070
PleI GAGTC 6 cut(s) 185, 324, 492, 807, 959, 1115
PpsI GAGTC 6 cut(s) 185, 324, 492, 807, 959, 1115
Ppu21I YACGTR 1 cut(s) 671
PpuMI RGGWCCY 1 cut(s) 938
PshBI ATTAAT 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 997
Psp5II RGGWCCY 1 cut(s) 938
Psp6I CCWGG 1 cut(s) 1116
PspGI CCWGG 1 cut(s) 1116
PspN4I GGNNCC 7 cut(s) 82, 297, 352, 567, 691, 775, 940
PspOMI GGGCCC 1 cut(s) 565
PspPI GGNCC 6 cut(s) 112, 565, 566, 665, 938, 1114
PspPPI RGGWCCY 1 cut(s) 938
PspXI VCTCGAGB 1 cut(s) 891
PsuI RGATCY 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 4 cut(s) 440, 632, 691, 782
RsaNI GTAC 4 cut(s) 439, 631, 690, 781
RseI CAYNNNNRTG 1 cut(s) 1109
SacI GAGCTC 1 cut(s) 997
SaqAI TTAA 2 cut(s) 11, 255
SatI GCNGC 3 cut(s) 111, 535, 1069
Sau3AI GATC 6 cut(s) 3, 60, 171, 322, 581, 796
Sau96I GGNCC 6 cut(s) 112, 565, 566, 665, 938, 1114
SchI GAGTC 6 cut(s) 185, 325, 492, 807, 960, 1115
ScrFI CCNGG 3 cut(s) 370, 714, 1118
SduI GDGCHC 5 cut(s) 184, 569, 986, 997, 1091
SfaNI GCATC 2 cut(s) 737, 877
Sfr274I CTCGAG 1 cut(s) 891
SinI GGWCC 2 cut(s) 665, 938
SlaI CTCGAG 1 cut(s) 891
SmiMI CAYNNNNRTG 1 cut(s) 1109
SmlI CTYRAG 3 cut(s) 99, 313, 891
SmoI CTYRAG 3 cut(s) 99, 313, 891
Sse9I AATT 9 cut(s) 8, 34, 282, 378, 396, 719, 910, 1044, 1074
SsiI CCGC 9 cut(s) 110, 226, 263, 299, 534, 576, 788, 930, 1068
SspMI CTAG 3 cut(s) 434, 662, 729
SstI GAGCTC 1 cut(s) 997
StyD4I CCNGG 3 cut(s) 368, 712, 1116
StyI CCWWGG 1 cut(s) 915
TaaI ACNGT 2 cut(s) 529, 743
TaiI ACGT 3 cut(s) 484, 673, 840
TaqI TCGA 3 cut(s) 580, 795, 892
TaqII GACCGA 1 cut(s) 944
TasI AATT 9 cut(s) 8, 34, 282, 378, 396, 719, 910, 1044, 1074
TatI WGTACW 1 cut(s) 780
TauI GCSGC 3 cut(s) 113, 537, 1071
TfiI GAWTC 4 cut(s) 137, 212, 240, 676
Tru1I TTAA 2 cut(s) 11, 255
Tru9I TTAA 2 cut(s) 11, 255
TscAI CASTG 5 cut(s) 45, 147, 184, 297, 532
TseFI GTSAC 3 cut(s) 40, 482, 682
Tsp45I GTSAC 3 cut(s) 40, 482, 682
TspDTI ATGAA 6 cut(s) 15, 662, 832, 868, 1076, 1109
TspRI CASTG 5 cut(s) 45, 147, 184, 297, 532
Van91I CCANNNNNTGG 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 665, 938
VspI ATTAAT 1 cut(s) 11
XapI RAATTY 3 cut(s) 34, 378, 396
XceI RCATGY 1 cut(s) 927
XhoI CTCGAG 1 cut(s) 891
XspI CTAG 3 cut(s) 434, 662, 729
Zsp2I ATGCAT 1 cut(s) 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.