Rw3G001170

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0018854
Physical Location & Seq
Reverse (-)
943319 .. 944829
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001170.1

Sequence Viewer

Length: 1128 bp
ATGATCCAATTAATGGCCACCTATTCATTATCAAATTCAGTGACGACTATATGTTGTCTGATCTTTTTGGCATTCTTGGGAACCATTTACCTTGCTCCTCAAGCAGAAGCGGCCCGAGCTTTCTTTGTTTTTGGTGATTCACTGGTTGATAATGGCAACAACAACTATCTGATCACCAGTGCTCGTGCCGACTCTTACCCCTACGGCATTGATTCTCCGACACACCGCCCTCCGGCAGATTCTCCAATGGCCTTAACATTGCGGACATCATCAAAAATTGGGTCAGTGGAACCGCCATTGCCATACTTGAGTCCGGATCTAAATGGAAGAAGGCTACTTATTGGTGCCAACTTCGCTTCAGCCGGGGTTGGAATTCTCAATGACACCGGAATTCAGTTTCTAAATATACTCCGAATGCCTATACAACTAGCGTACTTCCAAGAATACCAGAGAAGAGTGCAGGCTCTCATCGGACGTGACCAAGCCAAAAGACTCATAGAACAATCCCTCGTCCTTATCACTGTCGGCGGCAATGATTTCGTCAACAACTACTATTTGGGCCCTTCCTCCGCTCGATCTCGCCAACTTCCCCTCCCAAGATATGTCAAGTATGTCATATCCGAGTACGAGAAACTTTTGATGAACTTGTATAAGCTAGGACCACGTAGGATTCTGGTGACCGGTACCGGAGCAATGGGATGTGTCCCCGGAGAATTGGCGACTAGAGCCACGAACGGTGGATGCTCAGCTGAAGTTCAACGAGCCGGTGCCTTGTACAATCCGCAACTCGATCAAATGTTGAAAGGACTCAACAAGAAACTTGGGAAAGACGTTTTCATTTCTGCAAATAATGGACAAATGCATCTGGATTTCATCAACAACCCTCGAGCATTTGGGTTTACTAATTCCAAGGTTGCATGTTGCGGGCAAGGACCCTACAATGGAGTCGGTCTCTGCACATTTCTGTCCAACTTGTGCCCCAACAGAGCTCTCTATGCGTTTTGGGACGCCTTCCACCCATCTGAGAAGGCTAATAGAATTATTGTGCAGAAGATGAATAGCGGCTCAATTCGCTACATGAGCCCCATGAACCTCAGCACAATCATGGCCCTGGACTCTACTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

375

Amino Acids

41.09

Weight (kDa)

9.27

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 350 2e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018854)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01370
malus_domestica MD04G1234800.v1.1
rosa_chinensis RchiOBHm_Chr3g0448941
rosa_laevigata RLG00000025864
rosa_multiflora Rmu_sc0003116.1_g000015
rosa_roxburghii Rroxscaffold_6G00427120
rosa_rugosa Rorug02G0617200
rosa_samantha Rh3CG014800
rosa_wichuraiana Rw3G001170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 683
AccB1I GGYRCC 3 cut(s) 344, 683, 767
AccB7I CCANNNNNTGG 1 cut(s) 13
AccBSI CCGCTC 1 cut(s) 572
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 9 cut(s) 110, 226, 262, 293, 528, 570, 782, 924, 1062
AclWI GGATC 1 cut(s) 324
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 3 cut(s) 34, 372, 390
AcuI CTGAAG 2 cut(s) 342, 771
AcyI GRCGYC 1 cut(s) 1008
AfaI GTAC 4 cut(s) 434, 626, 685, 776
AfiI CCNNNNNNNGG 3 cut(s) 13, 232, 941
AgeI ACCGGT 1 cut(s) 680
AgsI TTSAA 2 cut(s) 758, 802
AjiI CACGTC 1 cut(s) 476
AjnI CCWGG 1 cut(s) 1110
AluBI AGCT 4 cut(s) 119, 655, 749, 989
AluI AGCT 4 cut(s) 119, 655, 749, 989
Alw21I GWGCWC 2 cut(s) 184, 991
Alw26I GTCTC 1 cut(s) 956
AlwI GGATC 1 cut(s) 324
Ama87I CYCGRG 2 cut(s) 114, 885
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 5 cut(s) 15, 111, 249, 559, 1107
ApaI GGGCCC 1 cut(s) 563
ApoI RAATTY 3 cut(s) 34, 372, 390
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
AseI ATTAAT 1 cut(s) 11
AsiGI ACCGGT 1 cut(s) 680
Asp718I GGTACC 1 cut(s) 683
AspS9I GGNCC 6 cut(s) 112, 559, 560, 659, 932, 1108
AsuC2I CCSGG 2 cut(s) 364, 708
AsuHPI GGTGA 3 cut(s) 146, 166, 688
AvaI CYCGRG 2 cut(s) 114, 885
AvaII GGWCC 2 cut(s) 659, 932
BaeGI GKGCMC 2 cut(s) 563, 980
BalI TGGCCA 1 cut(s) 17
BanI GGYRCC 3 cut(s) 344, 683, 767
BanII GRGCYC 3 cut(s) 563, 991, 1085
BauI CACGAG 1 cut(s) 183
Bbv12I GWGCWC 2 cut(s) 184, 991
BbvCI CCTCAGC 1 cut(s) 1094
BccI CCATC 1 cut(s) 1027
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 520, 554
BciT130I CCWGG 1 cut(s) 1112
BclI TGATCA 1 cut(s) 171
BcnI CCSGG 2 cut(s) 364, 708
BcoDI GTCTC 1 cut(s) 956
BfaI CTAG 3 cut(s) 428, 656, 723
BisI GCNGC 3 cut(s) 111, 529, 1063
BlpI GCTNAGC 1 cut(s) 745
BlsI GCNGC 3 cut(s) 112, 530, 1064
Bme1390I CCNGG 3 cut(s) 364, 708, 1112
Bme18I GGWCC 2 cut(s) 659, 932
BmeT110I CYCGRG 2 cut(s) 114, 885
BmgBI CACGTC 1 cut(s) 476
BmgT120I GGNCC 6 cut(s) 112, 559, 560, 659, 932, 1108
BmiI GGNNCC 7 cut(s) 82, 291, 346, 561, 685, 769, 934
BmrFI CCNGG 3 cut(s) 364, 708, 1112
BmsI GCATC 2 cut(s) 731, 871
BplI GAGNNNNNCTC 2 cut(s) 936, 968
Bpu10I CCTNAGC 1 cut(s) 1094
Bpu1102I GCTNAGC 1 cut(s) 745
BpuEI CTTGAG 2 cut(s) 84, 328
BpuMI CCSGG 2 cut(s) 364, 708
BsaAI YACGTR 1 cut(s) 665
BsaHI GRCGYC 1 cut(s) 1008
BsaI GGTCTC 1 cut(s) 956
BsaJI CCNNGG 4 cut(s) 363, 706, 909, 1110
BsaWI WCCGGW 4 cut(s) 313, 386, 680, 686
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 232, 941
Bse118I RCCGGY 2 cut(s) 680, 764
Bse1I ACTGG 2 cut(s) 147, 177
Bse3DI GCAATG 4 cut(s) 257, 296, 538, 699
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 1 cut(s) 1112
BseDI CCNNGG 4 cut(s) 363, 706, 909, 1110
BseGI GGATG 2 cut(s) 704, 746
BseLI CCNNNNNNNGG 3 cut(s) 13, 232, 941
BseMI GCAATG 4 cut(s) 257, 296, 538, 699
BseMII CTCAG 3 cut(s) 759, 1014, 1108
BseNI ACTGG 2 cut(s) 147, 177
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 2 cut(s) 563, 980
BsgI GTGCAG 3 cut(s) 479, 940, 1067
BshFI GGCC 5 cut(s) 17, 113, 251, 561, 1109
BshNI GGYRCC 3 cut(s) 344, 683, 767
BshTI ACCGGT 1 cut(s) 680
BsiHKAI GWGCWC 2 cut(s) 184, 991
BsiHKCI CYCGRG 2 cut(s) 114, 885
BsiSI CCGG 8 cut(s) 233, 314, 363, 387, 681, 687, 708, 765
BslFI GGGAC 2 cut(s) 689, 1019
BslI CCNNNNNNNGG 3 cut(s) 13, 232, 941
BsmAI GTCTC 1 cut(s) 956
BsmFI GGGAC 2 cut(s) 689, 1019
BsmI GAATGC 2 cut(s) 71, 420
BsnI GGCC 5 cut(s) 17, 113, 251, 561, 1109
Bso31I GGTCTC 1 cut(s) 956
BsoBI CYCGRG 2 cut(s) 114, 885
Bsp120I GGGCCC 1 cut(s) 559
Bsp1286I GDGCHC 5 cut(s) 184, 563, 980, 991, 1085
Bsp13I TCCGGA 1 cut(s) 313
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 6 cut(s) 3, 60, 171, 316, 575, 790
Bsp1720I GCTNAGC 1 cut(s) 745
BspACI CCGC 9 cut(s) 110, 226, 262, 293, 528, 570, 782, 924, 1062
BspANI GGCC 5 cut(s) 17, 113, 251, 561, 1109
BspCNI CTCAG 3 cut(s) 758, 1015, 1107
BspEI TCCGGA 1 cut(s) 313
BspLI GGNNCC 7 cut(s) 82, 291, 346, 561, 685, 769, 934
BspPI GGATC 1 cut(s) 324
BspT107I GGYRCC 3 cut(s) 344, 683, 767
BspTNI GGTCTC 1 cut(s) 956
BsrBI CCGCTC 1 cut(s) 572
BsrDI GCAATG 4 cut(s) 257, 296, 538, 699
BsrFI RCCGGY 2 cut(s) 680, 764
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 2 cut(s) 147, 177
BssAI RCCGGY 2 cut(s) 680, 764
BssECI CCNNGG 4 cut(s) 363, 706, 909, 1110
BssMI GATC 6 cut(s) 3, 60, 171, 316, 575, 790
BssNI GRCGYC 1 cut(s) 1008
BssSI CACGAG 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 909
Bst2BI CACGAG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 1112
Bst4CI ACNGT 2 cut(s) 523, 737
Bst6I CTCTTC 1 cut(s) 448
BstACI GRCGYC 1 cut(s) 1008
BstAUI TGTACA 1 cut(s) 774
BstBAI YACGTR 1 cut(s) 665
BstC8I GCNNGC 2 cut(s) 462, 926
BstDEI CTNAG 3 cut(s) 745, 1023, 1094
BstEII GGTNACC 1 cut(s) 676
BstF5I GGATG 2 cut(s) 704, 746
BstKTI GATC 6 cut(s) 6, 63, 174, 319, 578, 793
BstMAI GTCTC 1 cut(s) 956
BstMBI GATC 6 cut(s) 3, 60, 171, 316, 575, 790
BstMWI GCNNNNNNNGC 8 cut(s) 101, 110, 116, 353, 725, 995, 1071, 1080
BstNI CCWGG 1 cut(s) 1112
BstNSI RCATGY 1 cut(s) 921
BstPI GGTNACC 1 cut(s) 676
BstSCI CCNGG 3 cut(s) 362, 706, 1110
BstSLI GKGCMC 2 cut(s) 563, 980
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BsuRI GGCC 5 cut(s) 17, 113, 251, 561, 1109
BtrI CACGTC 1 cut(s) 476
BtsCI GGATG 2 cut(s) 704, 746
BtsIMutI CAGTG 5 cut(s) 45, 140, 184, 291, 519
Cac8I GCNNGC 2 cut(s) 462, 926
Cfr10I RCCGGY 2 cut(s) 680, 764
Cfr13I GGNCC 6 cut(s) 112, 559, 560, 659, 932, 1108
CseI GACGC 1 cut(s) 1016
Csp6I GTAC 4 cut(s) 433, 625, 684, 775
CspAI ACCGGT 1 cut(s) 680
CviAII CATG 4 cut(s) 918, 1078, 1087, 1105
CviQI GTAC 4 cut(s) 433, 625, 684, 775
DdeI CTNAG 3 cut(s) 745, 1023, 1094
DpnI GATC 6 cut(s) 5, 62, 173, 318, 577, 792
DpnII GATC 6 cut(s) 3, 60, 171, 316, 575, 790
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 448
Ecl136II GAGCTC 1 cut(s) 989
Eco130I CCWWGG 1 cut(s) 909
Eco24I GRGCYC 3 cut(s) 563, 991, 1085
Eco31I GGTCTC 1 cut(s) 956
Eco47I GGWCC 2 cut(s) 659, 932
Eco53kI GAGCTC 1 cut(s) 989
Eco57I CTGAAG 2 cut(s) 342, 771
Eco88I CYCGRG 2 cut(s) 114, 885
Eco91I GGTNACC 1 cut(s) 676
EcoICRI GAGCTC 1 cut(s) 989
EcoO109I RGGNCCY 2 cut(s) 560, 932
EcoO65I GGTNACC 1 cut(s) 676
EcoRI GAATTC 2 cut(s) 372, 390
EcoRII CCWGG 1 cut(s) 1110
EcoT14I CCWWGG 1 cut(s) 909
EcoT22I ATGCAT 1 cut(s) 864
EcoT38I GRGCYC 3 cut(s) 563, 991, 1085
ErhI CCWWGG 1 cut(s) 909
FaeI CATG 4 cut(s) 921, 1081, 1090, 1108
FaqI GGGAC 2 cut(s) 689, 1019
FatI CATG 4 cut(s) 917, 1077, 1086, 1104
FauI CCCGC 1 cut(s) 917
FbaI TGATCA 1 cut(s) 171
Fnu4HI GCNGC 3 cut(s) 111, 529, 1063
FokI GGATG 2 cut(s) 711, 753
FriOI GRGCYC 3 cut(s) 563, 991, 1085
Fsp4HI GCNGC 3 cut(s) 111, 529, 1063
FspBI CTAG 3 cut(s) 428, 656, 723
GluI GCNGC 3 cut(s) 111, 529, 1063
HaeIII GGCC 5 cut(s) 17, 113, 251, 561, 1109
HapII CCGG 8 cut(s) 233, 314, 363, 387, 681, 687, 708, 765
HgaI GACGC 1 cut(s) 1016
Hin1I GRCGYC 1 cut(s) 1008
Hin1II CATG 4 cut(s) 921, 1081, 1090, 1108
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HpaII CCGG 8 cut(s) 233, 314, 363, 387, 681, 687, 708, 765
HphI GGTGA 3 cut(s) 146, 166, 688
Hpy166II GTNNAC 2 cut(s) 544, 900
Hpy188I TCNGA 7 cut(s) 60, 171, 219, 413, 473, 622, 1024
Hpy188III TCNNGA 2 cut(s) 314, 866
Hpy8I GTNNAC 2 cut(s) 544, 900
HpyAV CCTTC 4 cut(s) 324, 573, 1021, 1021
HpyCH4III ACNGT 2 cut(s) 523, 737
HpyCH4IV ACGT 3 cut(s) 475, 664, 831
HpyCH4V TGCA 6 cut(s) 460, 845, 862, 917, 957, 1048
HpyF10VI GCNNNNNNNGC 8 cut(s) 101, 110, 116, 353, 725, 995, 1071, 1080
HpyF3I CTNAG 3 cut(s) 745, 1023, 1094
HpySE526I ACGT 3 cut(s) 475, 664, 831
Hsp92I GRCGYC 1 cut(s) 1008
Hsp92II CATG 4 cut(s) 921, 1081, 1090, 1108
Kpn2I TCCGGA 1 cut(s) 313
KpnI GGTACC 1 cut(s) 687
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 6 cut(s) 3, 60, 171, 316, 575, 790
LmnI GCTCC 2 cut(s) 100, 689
LweI GCATC 2 cut(s) 731, 871
MaeI CTAG 3 cut(s) 428, 656, 723
MaeII ACGT 3 cut(s) 475, 664, 831
MaeIII GTNAC 3 cut(s) 40, 476, 676
MalI GATC 6 cut(s) 5, 62, 173, 318, 577, 792
MbiI CCGCTC 1 cut(s) 572
MboI GATC 6 cut(s) 3, 60, 171, 316, 575, 790
MboII GAAGA 3 cut(s) 339, 465, 1063
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 5 cut(s) 184, 563, 980, 991, 1085
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 9 cut(s) 8, 34, 276, 372, 390, 713, 904, 1038, 1068
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 6 cut(s) 185, 319, 486, 801, 954, 1109
MmeI TCCRAC 3 cut(s) 242, 349, 993
MnlI CCTC 7 cut(s) 108, 240, 518, 577, 602, 894, 1103
Mox20I TGGCCA 1 cut(s) 17
Mph1103I ATGCAT 1 cut(s) 864
MroI TCCGGA 1 cut(s) 313
MscI TGGCCA 1 cut(s) 17
MseI TTAA 2 cut(s) 11, 254
MslI CAYNNNNRTG 1 cut(s) 1103
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 8 cut(s) 233, 314, 363, 387, 681, 687, 708, 765
MspR9I CCNGG 3 cut(s) 364, 708, 1112
Mva1269I GAATGC 2 cut(s) 71, 420
MvaI CCWGG 1 cut(s) 1112
MwoI GCNNNNNNNGC 8 cut(s) 101, 110, 116, 353, 725, 995, 1071, 1080
NciI CCSGG 2 cut(s) 364, 708
NdeII GATC 6 cut(s) 3, 60, 171, 316, 575, 790
NlaIII CATG 4 cut(s) 921, 1081, 1090, 1108
NlaIV GGNNCC 7 cut(s) 82, 291, 346, 561, 685, 769, 934
NmuCI GTSAC 3 cut(s) 40, 476, 676
NsiI ATGCAT 1 cut(s) 864
NspI RCATGY 1 cut(s) 921
PaeR7I CTCGAG 1 cut(s) 885
PctI GAATGC 2 cut(s) 71, 420
PfeI GAWTC 4 cut(s) 137, 212, 239, 670
PflMI CCANNNNNTGG 1 cut(s) 13
PinAI ACCGGT 1 cut(s) 680
PkrI GCNGC 3 cut(s) 112, 530, 1064
PleI GAGTC 6 cut(s) 185, 318, 486, 801, 953, 1109
PpsI GAGTC 6 cut(s) 185, 318, 486, 801, 953, 1109
Ppu21I YACGTR 1 cut(s) 665
PpuMI RGGWCCY 1 cut(s) 932
PshBI ATTAAT 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 991
Psp5II RGGWCCY 1 cut(s) 932
Psp6I CCWGG 1 cut(s) 1110
PspEI GGTNACC 1 cut(s) 676
PspGI CCWGG 1 cut(s) 1110
PspN4I GGNNCC 7 cut(s) 82, 291, 346, 561, 685, 769, 934
PspOMI GGGCCC 1 cut(s) 559
PspPI GGNCC 6 cut(s) 112, 559, 560, 659, 932, 1108
PspPPI RGGWCCY 1 cut(s) 932
PspXI VCTCGAGB 1 cut(s) 885
PsuI RGATCY 1 cut(s) 316
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 4 cut(s) 434, 626, 685, 776
RsaNI GTAC 4 cut(s) 433, 625, 684, 775
RseI CAYNNNNRTG 1 cut(s) 1103
SacI GAGCTC 1 cut(s) 991
SaqAI TTAA 2 cut(s) 11, 254
SatI GCNGC 3 cut(s) 111, 529, 1063
Sau3AI GATC 6 cut(s) 3, 60, 171, 316, 575, 790
Sau96I GGNCC 6 cut(s) 112, 559, 560, 659, 932, 1108
SchI GAGTC 6 cut(s) 185, 319, 486, 801, 954, 1109
ScrFI CCNGG 3 cut(s) 364, 708, 1112
SduI GDGCHC 5 cut(s) 184, 563, 980, 991, 1085
SfaNI GCATC 2 cut(s) 731, 871
Sfr274I CTCGAG 1 cut(s) 885
SinI GGWCC 2 cut(s) 659, 932
SlaI CTCGAG 1 cut(s) 885
SmiMI CAYNNNNRTG 1 cut(s) 1103
SmlI CTYRAG 3 cut(s) 99, 307, 885
SmoI CTYRAG 3 cut(s) 99, 307, 885
Sse9I AATT 9 cut(s) 8, 34, 276, 372, 390, 713, 904, 1038, 1068
SsiI CCGC 9 cut(s) 110, 226, 262, 293, 528, 570, 782, 924, 1062
SspMI CTAG 3 cut(s) 428, 656, 723
SstI GAGCTC 1 cut(s) 991
StyD4I CCNGG 3 cut(s) 362, 706, 1110
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 2 cut(s) 523, 737
TaiI ACGT 3 cut(s) 478, 667, 834
TaqI TCGA 3 cut(s) 574, 789, 886
TaqII GACCGA 1 cut(s) 938
TasI AATT 9 cut(s) 8, 34, 276, 372, 390, 713, 904, 1038, 1068
TatI WGTACW 1 cut(s) 774
TauI GCSGC 3 cut(s) 113, 531, 1065
TfiI GAWTC 4 cut(s) 137, 212, 239, 670
Tru1I TTAA 2 cut(s) 11, 254
Tru9I TTAA 2 cut(s) 11, 254
TscAI CASTG 5 cut(s) 45, 147, 184, 291, 526
TseFI GTSAC 3 cut(s) 40, 476, 676
Tsp45I GTSAC 3 cut(s) 40, 476, 676
TspDTI ATGAA 6 cut(s) 15, 656, 826, 862, 1070, 1103
TspRI CASTG 5 cut(s) 45, 147, 184, 291, 526
Van91I CCANNNNNTGG 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 659, 932
VspI ATTAAT 1 cut(s) 11
XapI RAATTY 3 cut(s) 34, 372, 390
XceI RCATGY 1 cut(s) 921
XhoI CTCGAG 1 cut(s) 885
XspI CTAG 3 cut(s) 428, 656, 723
Zsp2I ATGCAT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.