MD04G1234800.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0018854
Physical Location & Seq
Forward (+)
31397947 .. 31400212
2266 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1234800.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGCTGCAGCCTCTTCATCGTCGATGATTTTTACATGCCGACTGATGATTTACTTGATTGTTATTGTTAGTAATTACAGTATTGGTCCTCAAATGGTTCAAGTAGCAGAGGGTAGTCGAGCATTCTTTGTTTTTGGCGATTCACTGGTCGACAATGGCAACAACAACTATCTGATATCGATTGCTCGAGCCGACTCTTACCCTTATGGAATCGATTTTCCAACTCACCGTCCCACCGGCCGTTTCTCTAATGGCCTCATCATCCCGGACCTTATCAGTCAGAAAATTGGCTCAAGAGAACCCCCAATGCCATACTTGAGTCCACAGCTTAACGGACGAAGGCTACTTGTTGGTGCAAACTTCGCTTCCGCGGGAGTTGGAATTCTCAATGAAACTGGCGCTCAGTTTCTAGATATACTCAGAATGTTTAGGCAACTGAACTACTTCAAAGAATACCAGCAAAGAGTGCAAGCCCTGGTCGGAAAGCAGCAGACCAGAAGACTCATAAGCCAATCACTTGTCCTCATCACCGTCGGCGGCAACGATTTCGTCAACAACTACTACTCAGCCTCTTCCATTTCTCAATCTCGCAACCTCCCCCTCCCTACTTACGTCAAGCTTCTCATCTCTGAGTACCGAAAAATTCTCATGAATTTATATCAACTCGGAGCACGTAGAGTTCTAGTTACTGGTACAGGACCGATGGGATGTGCTCCGGCAGAGTTGGCGGCTAGAAGCACGAATGGAGGATGCTCGGCTGAACTTCTACGAGCTGCTGCCCTGTACAACCCACAACTCACTCAAATGTTGGCCGGACTCAACAAGAAACTTGGCAAAGATGTATTCATATCTGCCAACACTAGGCAAATGCATGCAGATTTCATCAATAACCCCCAAGCATTTGGCTTTACCACATCGAAGGTAGCATGTTGTGGGCAAGGACCCTACAATGGACAAGGTCTCTGCACATTTCTGTCCAATTTGTGCCCAAACAGAAACCTGTATGCGTTTTGGGATGCCTTTCATCCATCTGAAAAGGCTAATAGAATGTATGCAGAGATGATCACCACAGGCTCAACTGACTATATGAGCCCCATGAACCTCAGTACTATCCTGGCCTTGGACTCCACCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

379

Amino Acids

41.52

Weight (kDa)

9.35

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 353 2e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018854)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01370
malus_domestica MD04G1234800.v1.1
rosa_chinensis RchiOBHm_Chr3g0448941
rosa_laevigata RLG00000025864
rosa_multiflora Rmu_sc0003116.1_g000015
rosa_roxburghii Rroxscaffold_6G00427120
rosa_rugosa Rorug02G0617200
rosa_samantha Rh3CG014800
rosa_wichuraiana Rw3G001170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 150
AccII CGCG 1 cut(s) 371
AciI CCGC 4 cut(s) 369, 371, 537, 728
AcoI YGGCCR 2 cut(s) 238, 810
AcsI RAATTY 3 cut(s) 381, 642, 652
AfaI GTAC 4 cut(s) 635, 694, 785, 1108
AfiI CCNNNNNNNGG 3 cut(s) 861, 950, 1120
AgsI TTSAA 2 cut(s) 101, 448
AjnI CCWGG 2 cut(s) 474, 1113
AluBI AGCT 3 cut(s) 328, 619, 773
AluI AGCT 3 cut(s) 328, 619, 773
Alw21I GWGCWC 2 cut(s) 673, 715
Alw26I GTCTC 1 cut(s) 965
Ama87I CYCGRG 1 cut(s) 186
AoxI GGCC 4 cut(s) 238, 253, 810, 1116
ApeKI GCWGC 5 cut(s) 5, 8, 487, 773, 776
ApoI RAATTY 3 cut(s) 381, 642, 652
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 4 cut(s) 86, 268, 698, 941
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 3 cut(s) 218, 520, 1057
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 4 cut(s) 86, 268, 698, 941
BaeGI GKGCMC 1 cut(s) 989
BanII GRGCYC 1 cut(s) 1094
BbsI GAAGAC 1 cut(s) 505
Bbv12I GWGCWC 2 cut(s) 673, 715
BbvI GCAGC 4 cut(s) 20, 499, 760, 763
BccI CCATC 2 cut(s) 697, 1036
BceAI ACGGC 1 cut(s) 225
BcgI CGANNNNNNTGC 2 cut(s) 529, 563
BciT130I CCWGG 2 cut(s) 476, 1115
BclI TGATCA 1 cut(s) 1062
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 965
BfaI CTAG 4 cut(s) 410, 683, 732, 861
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 402
BisI GCNGC 7 cut(s) 6, 9, 488, 538, 729, 774, 777
BlsI GCNGC 7 cut(s) 7, 10, 489, 539, 730, 775, 778
BmcAI AGTACT 1 cut(s) 1108
Bme1390I CCNGG 3 cut(s) 266, 476, 1115
Bme18I GGWCC 4 cut(s) 86, 268, 698, 941
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 4 cut(s) 86, 268, 698, 941
BmiI GGNNCC 1 cut(s) 943
BmrFI CCNGG 3 cut(s) 266, 476, 1115
BmsI GCATC 2 cut(s) 740, 1006
BpiI GAAGAC 1 cut(s) 505
BpuEI CTTGAG 2 cut(s) 277, 337
BpuMI CCSGG 1 cut(s) 266
Bsa29I ATCGAT 2 cut(s) 179, 213
BsaAI YACGTR 1 cut(s) 674
BsaI GGTCTC 1 cut(s) 965
BsaJI CCNNGG 3 cut(s) 369, 474, 1119
Bsc4I CCNNNNNNNGG 3 cut(s) 861, 950, 1120
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 3 cut(s) 150, 400, 694
BseBI CCWGG 2 cut(s) 476, 1115
BseCI ATCGAT 2 cut(s) 179, 213
BseDI CCNNGG 3 cut(s) 369, 474, 1119
BseGI GGATG 5 cut(s) 261, 713, 755, 1021, 1024
BseLI CCNNNNNNNGG 3 cut(s) 861, 950, 1120
BseMII CTCAG 5 cut(s) 416, 433, 579, 621, 1117
BseNI ACTGG 3 cut(s) 150, 400, 694
BseSI GKGCMC 1 cut(s) 989
BseX3I CGGCCG 1 cut(s) 238
BseXI GCAGC 4 cut(s) 20, 499, 760, 763
BsgI GTGCAG 1 cut(s) 949
Bsh1236I CGCG 1 cut(s) 371
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 4 cut(s) 240, 255, 812, 1118
BshVI ATCGAT 2 cut(s) 179, 213
BsiEI CGRYCG 1 cut(s) 241
BsiHKAI GWGCWC 2 cut(s) 673, 715
BsiHKCI CYCGRG 1 cut(s) 186
BsiSI CCGG 4 cut(s) 237, 266, 716, 813
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 3 cut(s) 861, 950, 1120
BsmAI GTCTC 1 cut(s) 965
BsmFI GGGAC 1 cut(s) 216
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 4 cut(s) 240, 255, 812, 1118
Bso31I GGTCTC 1 cut(s) 965
BsoBI CYCGRG 1 cut(s) 186
Bsp1286I GDGCHC 4 cut(s) 673, 715, 989, 1094
Bsp1407I TGTACA 1 cut(s) 783
Bsp143I GATC 1 cut(s) 1062
BspACI CCGC 4 cut(s) 369, 371, 537, 728
BspANI GGCC 4 cut(s) 240, 255, 812, 1118
BspCNI CTCAG 5 cut(s) 415, 432, 578, 622, 1116
BspDI ATCGAT 2 cut(s) 179, 213
BspFNI CGCG 1 cut(s) 371
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 1 cut(s) 943
BspMAI CTGCAG 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 965
BsrFI RCCGGY 1 cut(s) 236
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 3 cut(s) 150, 400, 694
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 3 cut(s) 369, 474, 1119
BssMI GATC 1 cut(s) 1062
BssT1I CCWWGG 1 cut(s) 1119
Bst2UI CCWGG 2 cut(s) 476, 1115
Bst4CI ACNGT 3 cut(s) 80, 230, 532
Bst6I CTCTTC 2 cut(s) 19, 577
BstAPI GCANNNNNTGC 1 cut(s) 466
BstAUI TGTACA 1 cut(s) 783
BstBAI YACGTR 1 cut(s) 674
BstC8I GCNNGC 2 cut(s) 471, 873
BstDEI CTNAG 5 cut(s) 402, 419, 565, 630, 1103
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 5 cut(s) 261, 713, 755, 1021, 1024
BstFNI CGCG 1 cut(s) 371
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 1 cut(s) 1065
BstMAI GTCTC 1 cut(s) 965
BstMBI GATC 1 cut(s) 1062
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 3 cut(s) 362, 466, 725
BstNI CCWGG 2 cut(s) 476, 1115
BstNSI RCATGY 3 cut(s) 39, 875, 930
BstSCI CCNGG 3 cut(s) 264, 474, 1113
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 989
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 4 cut(s) 20, 499, 760, 763
BstV2I GAAGAC 1 cut(s) 505
BstXI CCANNNNNNTGG 1 cut(s) 902
BstZI CGGCCG 1 cut(s) 238
Bsu15I ATCGAT 2 cut(s) 179, 213
BsuRI GGCC 4 cut(s) 240, 255, 812, 1118
BsuTUI ATCGAT 2 cut(s) 179, 213
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 5 cut(s) 261, 713, 755, 1021, 1024
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 471, 873
CciI TCATGA 1 cut(s) 648
CfoI GCGC 1 cut(s) 401
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 4 cut(s) 86, 268, 698, 941
Cfr42I CCGCGG 1 cut(s) 372
ClaI ATCGAT 2 cut(s) 179, 213
Csp6I GTAC 4 cut(s) 634, 693, 784, 1107
CviAII CATG 5 cut(s) 36, 649, 872, 927, 1096
CviQI GTAC 4 cut(s) 634, 693, 784, 1107
DdeI CTNAG 5 cut(s) 402, 419, 565, 630, 1103
DpnI GATC 1 cut(s) 1064
DpnII GATC 1 cut(s) 1062
EaeI YGGCCR 2 cut(s) 238, 810
EagI CGGCCG 1 cut(s) 238
Eam1104I CTCTTC 2 cut(s) 19, 577
EarI CTCTTC 2 cut(s) 19, 577
EclXI CGGCCG 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 1119
Eco24I GRGCYC 1 cut(s) 1094
Eco31I GGTCTC 1 cut(s) 965
Eco32I GATATC 1 cut(s) 177
Eco47I GGWCC 4 cut(s) 86, 268, 698, 941
Eco52I CGGCCG 1 cut(s) 238
Eco88I CYCGRG 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 941
EcoRI GAATTC 1 cut(s) 381
EcoRII CCWGG 2 cut(s) 474, 1113
EcoRV GATATC 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 1119
EcoT22I ATGCAT 1 cut(s) 873
EcoT38I GRGCYC 1 cut(s) 1094
ErhI CCWWGG 1 cut(s) 1119
FaeI CATG 5 cut(s) 39, 652, 875, 930, 1099
FaqI GGGAC 1 cut(s) 216
FatI CATG 5 cut(s) 35, 648, 871, 926, 1095
FauI CCCGC 1 cut(s) 364
FbaI TGATCA 1 cut(s) 1062
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 7 cut(s) 6, 9, 488, 538, 729, 774, 777
FokI GGATG 5 cut(s) 248, 720, 762, 1011, 1028
FriOI GRGCYC 1 cut(s) 1094
Fsp4HI GCNGC 7 cut(s) 6, 9, 488, 538, 729, 774, 777
FspBI CTAG 4 cut(s) 410, 683, 732, 861
GlaI GCGC 1 cut(s) 400
GluI GCNGC 7 cut(s) 6, 9, 488, 538, 729, 774, 777
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 4 cut(s) 240, 255, 812, 1118
HapII CCGG 4 cut(s) 237, 266, 716, 813
HhaI GCGC 1 cut(s) 401
Hin1II CATG 5 cut(s) 39, 652, 875, 930, 1099
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HincII GTYRAC 2 cut(s) 151, 553
HindII GTYRAC 2 cut(s) 151, 553
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 7 cut(s) 140, 194, 210, 319, 501, 816, 1124
HpaII CCGG 4 cut(s) 237, 266, 716, 813
HphI GGTGA 3 cut(s) 218, 520, 1057
Hpy166II GTNNAC 3 cut(s) 151, 323, 553
Hpy188I TCNGA 7 cut(s) 174, 282, 422, 482, 631, 668, 1033
Hpy188III TCNNGA 3 cut(s) 294, 410, 649
Hpy8I GTNNAC 3 cut(s) 151, 323, 553
Hpy99I CGWCG 2 cut(s) 25, 536
HpyAV CCTTC 2 cut(s) 333, 913
HpyCH4III ACNGT 3 cut(s) 80, 230, 532
HpyCH4IV ACGT 2 cut(s) 612, 673
HpyCH4V TGCA 7 cut(s) 8, 356, 469, 871, 875, 966, 1055
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 466, 725
HpyF3I CTNAG 5 cut(s) 402, 419, 565, 630, 1103
HpySE526I ACGT 2 cut(s) 612, 673
Hsp92II CATG 5 cut(s) 39, 652, 875, 930, 1099
HspAI GCGC 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 1062
KspI CCGCGG 1 cut(s) 372
Kzo9I GATC 1 cut(s) 1062
LmnI GCTCC 2 cut(s) 668, 718
Lsp1109I GCAGC 4 cut(s) 20, 499, 760, 763
LweI GCATC 2 cut(s) 740, 1006
MaeI CTAG 4 cut(s) 410, 683, 732, 861
MaeII ACGT 2 cut(s) 612, 673
MaeIII GTNAC 1 cut(s) 685
MalI GATC 1 cut(s) 1064
MboI GATC 1 cut(s) 1062
MboII GAAGA 3 cut(s) 6, 510, 564
MhlI GDGCHC 4 cut(s) 673, 715, 989, 1094
MluCI AATT 6 cut(s) 73, 285, 381, 642, 652, 979
MlyI GAGTC 5 cut(s) 188, 328, 495, 810, 1118
MmeI TCCRAC 3 cut(s) 245, 358, 460
Mph1103I ATGCAT 1 cut(s) 873
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 803
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 4 cut(s) 237, 266, 716, 813
MspR9I CCNGG 3 cut(s) 266, 476, 1115
Mva1269I GAATGC 1 cut(s) 122
MvaI CCWGG 2 cut(s) 476, 1115
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 3 cut(s) 362, 466, 725
NciI CCSGG 1 cut(s) 266
NdeII GATC 1 cut(s) 1062
NlaIII CATG 5 cut(s) 39, 652, 875, 930, 1099
NlaIV GGNNCC 1 cut(s) 943
NmeAIII GCCGAG 1 cut(s) 734
NsiI ATGCAT 1 cut(s) 873
NspI RCATGY 3 cut(s) 39, 875, 930
PaeI GCATGC 1 cut(s) 875
PaeR7I CTCGAG 1 cut(s) 186
PagI TCATGA 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PctI GAATGC 1 cut(s) 122
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 2 cut(s) 140, 210
PflFI GACNNNGTC 1 cut(s) 957
PfoI TCCNGGA 1 cut(s) 264
PkrI GCNGC 7 cut(s) 7, 10, 489, 539, 730, 775, 778
PleI GAGTC 5 cut(s) 188, 327, 495, 810, 1118
PpsI GAGTC 5 cut(s) 188, 327, 495, 810, 1118
Ppu21I YACGTR 1 cut(s) 674
PpuMI RGGWCCY 1 cut(s) 941
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 2 cut(s) 474, 1113
PspGI CCWGG 2 cut(s) 474, 1113
PspN4I GGNNCC 1 cut(s) 943
PspPI GGNCC 4 cut(s) 86, 268, 698, 941
PspPPI RGGWCCY 1 cut(s) 941
PspXI VCTCGAGB 1 cut(s) 186
PstI CTGCAG 1 cut(s) 10
PsyI GACNNNGTC 1 cut(s) 957
RsaI GTAC 4 cut(s) 635, 694, 785, 1108
RsaNI GTAC 4 cut(s) 634, 693, 784, 1107
RseI CAYNNNNRTG 1 cut(s) 803
SacII CCGCGG 1 cut(s) 372
SalI GTCGAC 1 cut(s) 149
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 7 cut(s) 6, 9, 488, 538, 729, 774, 777
Sau3AI GATC 1 cut(s) 1062
Sau96I GGNCC 4 cut(s) 86, 268, 698, 941
ScaI AGTACT 1 cut(s) 1108
SchI GAGTC 5 cut(s) 188, 328, 495, 810, 1118
ScrFI CCNGG 3 cut(s) 266, 476, 1115
SduI GDGCHC 4 cut(s) 673, 715, 989, 1094
SfaNI GCATC 2 cut(s) 740, 1006
SfcI CTRYAG 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 186
Sfr303I CCGCGG 1 cut(s) 372
SgrBI CCGCGG 1 cut(s) 372
SinI GGWCC 4 cut(s) 86, 268, 698, 941
SlaI CTCGAG 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 803
SmlI CTYRAG 3 cut(s) 186, 292, 316
SmoI CTYRAG 3 cut(s) 186, 292, 316
SphI GCATGC 1 cut(s) 875
Sse9I AATT 6 cut(s) 73, 285, 381, 642, 652, 979
SsiI CCGC 4 cut(s) 369, 371, 537, 728
SspMI CTAG 4 cut(s) 410, 683, 732, 861
StyD4I CCNGG 3 cut(s) 264, 474, 1113
StyI CCWWGG 1 cut(s) 1119
TaaI ACNGT 3 cut(s) 80, 230, 532
TaiI ACGT 2 cut(s) 615, 676
TaqI TCGA 7 cut(s) 23, 118, 150, 179, 187, 213, 917
TaqII GACCGA 1 cut(s) 715
TasI AATT 6 cut(s) 73, 285, 381, 642, 652, 979
TatI WGTACW 2 cut(s) 783, 1106
TauI GCSGC 2 cut(s) 540, 731
TfiI GAWTC 2 cut(s) 140, 210
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 150
TseI GCWGC 5 cut(s) 5, 8, 487, 773, 776
TspDTI ATGAA 7 cut(s) 6, 405, 665, 835, 871, 1013, 1112
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 1 cut(s) 150
Tth111I GACNNNGTC 1 cut(s) 957
VpaK11BI GGWCC 4 cut(s) 86, 268, 698, 941
XapI RAATTY 3 cut(s) 381, 642, 652
XbaI TCTAGA 1 cut(s) 409
XceI RCATGY 3 cut(s) 39, 875, 930
XhoI CTCGAG 1 cut(s) 186
XmiI GTMKAC 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 4 cut(s) 410, 683, 732, 861
ZrmI AGTACT 1 cut(s) 1108
Zsp2I ATGCAT 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.