FvH4_6g03460

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016114
Physical Location & Seq
Reverse (-)
1902908 .. 1903399
492 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g03460.t1

Sequence Viewer

Length: 417 bp
ATGAAGCCCAGTGTTGTTCTGCATGCCCTAAACGACTCCACGGTGGCCGCATTTGTTGACGACACGACGCCGTTCGAGAAATGCAGCAAGGAATGCTTCGACGAGCTCGACGTTGACGCCGACGGCGTGTTGTCGAGGGAGGAGCTCCGTTCTGGTTTTGGTAATCTGCTGCCGGGAATAGGGTACTTGTCTCAGCCCAAAGAGGAGTTGAATAGTTTGCATGATGCGATATTCGAGAGGTTCGATGCGGATGGAAACGGCGTCGTTGATAGGGGTGAGTTTAAGGAGCTGTTGAGGGAGATCATGCTTGGGGTGGCGCGTGGGATTGGGGGGTCGCCGGTGCTGGTGGCGCTTGAGCATGGCAGTCTGCTTATGAGGGCGGCTCAACATGAACATGAACAGAATACGAAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

139

Amino Acids

15.11

Weight (kDa)

4.68

Isoelectric Point (pI)

26.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 30 - 98 3.4e-10 EF-hand domain pair
EF-hand_1 PF00036 75 - 100 1.7e-06 EF hand domain
EF-hand_6 PF13405 77 - 102 1e-05 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54530
fragaria_vesca FvH4_6g03460
malus_domestica MD04G1220600.v1.1
prunus_persica Prupe.6G339600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451951
rosa_laevigata RLG00000025634
rosa_multiflora Rmu_sc0006510.1_g000001
rosa_roxburghii Rroxscaffold_164G00436160 Rroxscaffold_6G00429130
rosa_rugosa Rorug02G0637800
rosa_samantha Rh3AG039400 Rh3BG040800 Rh3CG039300 Rh3DG040000
rosa_wichuraiana Rw3G002960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 319
AciI CCGC 3 cut(s) 48, 248, 380
AcoI YGGCCR 1 cut(s) 45
AcyI GRCGYC 3 cut(s) 68, 117, 261
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 1 cut(s) 211
AluBI AGCT 3 cut(s) 106, 145, 289
AluI AGCT 3 cut(s) 106, 145, 289
Alw21I GWGCWC 2 cut(s) 108, 147
Alw26I GTCTC 1 cut(s) 195
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 2 cut(s) 84, 169
AspLEI GCGC 2 cut(s) 319, 352
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 287
BanII GRGCYC 2 cut(s) 108, 147
Bbv12I GWGCWC 2 cut(s) 108, 147
BbvI GCAGC 2 cut(s) 96, 156
BccI CCATC 1 cut(s) 245
BceAI ACGGC 3 cut(s) 55, 139, 274
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 353
BisI GCNGC 4 cut(s) 48, 85, 170, 381
BlsI GCNGC 4 cut(s) 49, 86, 171, 382
Bme1390I CCNGG 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 174
BmrI ACTGGG 1 cut(s) 3
BmsI GCATC 2 cut(s) 214, 235
BmuI ACTGGG 1 cut(s) 3
BplI GAGNNNNNCTC 2 cut(s) 367, 399
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 174
BsaHI GRCGYC 3 cut(s) 68, 117, 261
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 9
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMII CTCAG 1 cut(s) 206
BseNI ACTGG 1 cut(s) 9
BseRI GAGGAG 2 cut(s) 155, 218
BseXI GCAGC 2 cut(s) 96, 156
Bsh1236I CGCG 1 cut(s) 319
BshFI GGCC 1 cut(s) 47
BsiHKAI GWGCWC 2 cut(s) 108, 147
BsiSI CCGG 2 cut(s) 173, 338
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 195
BsmI GAATGC 1 cut(s) 98
BsnI GGCC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 108, 147
Bsp143I GATC 1 cut(s) 300
BspACI CCGC 3 cut(s) 48, 248, 380
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 205
BspFNI CGCG 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 337
BsrI ACTGG 1 cut(s) 9
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 1 cut(s) 300
BssNI GRCGYC 3 cut(s) 68, 117, 261
Bst4CI ACNGT 1 cut(s) 43
BstACI GRCGYC 3 cut(s) 68, 117, 261
BstAPI GCANNNNNTGC 1 cut(s) 93
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 1 cut(s) 192
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 256
BstFNI CGCG 1 cut(s) 319
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 2 cut(s) 319, 352
BstKTI GATC 1 cut(s) 303
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 1 cut(s) 300
BstMWI GCNNNNNNNGC 2 cut(s) 93, 349
BstNSI RCATGY 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 172
BstUI CGCG 1 cut(s) 319
BstV1I GCAGC 2 cut(s) 96, 156
BsuRI GGCC 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 2 cut(s) 319, 352
Cfr10I RCCGGY 1 cut(s) 337
CseI GACGC 3 cut(s) 76, 125, 250
Csp6I GTAC 1 cut(s) 184
CviAII CATG 6 cut(s) 23, 221, 304, 359, 389, 395
CviJI RGCY 7 cut(s) 7, 47, 106, 145, 196, 289, 383
CviKI_1 RGCY 7 cut(s) 7, 47, 106, 145, 196, 289, 383
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 1 cut(s) 192
DpnI GATC 1 cut(s) 302
DpnII GATC 1 cut(s) 300
EaeI YGGCCR 1 cut(s) 45
Ecl136II GAGCTC 2 cut(s) 106, 145
Eco24I GRGCYC 2 cut(s) 108, 147
Eco53kI GAGCTC 2 cut(s) 106, 145
EcoICRI GAGCTC 2 cut(s) 106, 145
EcoT38I GRGCYC 2 cut(s) 108, 147
FaeI CATG 6 cut(s) 26, 224, 307, 362, 392, 398
FaiI YATR 7 cut(s) 24, 222, 305, 360, 374, 390, 396
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FatI CATG 6 cut(s) 22, 220, 303, 358, 388, 394
Fnu4HI GCNGC 4 cut(s) 48, 85, 170, 381
FokI GGATG 1 cut(s) 263
FriOI GRGCYC 2 cut(s) 108, 147
Fsp4HI GCNGC 4 cut(s) 48, 85, 170, 381
GlaI GCGC 2 cut(s) 318, 351
GluI GCNGC 4 cut(s) 48, 85, 170, 381
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 1 cut(s) 47
HapII CCGG 2 cut(s) 173, 338
HgaI GACGC 3 cut(s) 76, 125, 250
HhaI GCGC 2 cut(s) 319, 352
Hin1I GRCGYC 3 cut(s) 68, 117, 261
Hin1II CATG 6 cut(s) 26, 224, 307, 362, 392, 398
Hin6I GCGC 2 cut(s) 317, 350
HinP1I GCGC 2 cut(s) 317, 350
HincII GTYRAC 2 cut(s) 58, 115
HindII GTYRAC 2 cut(s) 58, 115
HinfI GANTC 1 cut(s) 35
HpaII CCGG 2 cut(s) 173, 338
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 2 cut(s) 58, 115
Hpy188III TCNNGA 2 cut(s) 76, 235
Hpy8I GTNNAC 2 cut(s) 58, 115
Hpy99I CGWCG 5 cut(s) 70, 104, 113, 125, 266
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 3 cut(s) 22, 84, 220
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 349
HpyF3I CTNAG 1 cut(s) 192
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 3 cut(s) 68, 117, 261
Hsp92II CATG 6 cut(s) 26, 224, 307, 362, 392, 398
HspAI GCGC 2 cut(s) 317, 350
Kzo9I GATC 1 cut(s) 300
LmnI GCTCC 3 cut(s) 142, 150, 286
LpnPI CCDG 5 cut(s) 22, 138, 186, 329, 351
Lsp1109I GCAGC 2 cut(s) 96, 156
LweI GCATC 2 cut(s) 214, 235
MaeII ACGT 1 cut(s) 111
MalI GATC 1 cut(s) 302
MboI GATC 1 cut(s) 300
MhlI GDGCHC 2 cut(s) 108, 147
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 6 cut(s) 129, 133, 196, 231, 288, 369
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 393
MspI CCGG 2 cut(s) 173, 338
MspR9I CCNGG 1 cut(s) 174
Mva1269I GAATGC 1 cut(s) 98
MvnI CGCG 1 cut(s) 319
MwoI GCNNNNNNNGC 2 cut(s) 93, 349
NciI CCSGG 1 cut(s) 174
NdeII GATC 1 cut(s) 300
NlaIII CATG 6 cut(s) 26, 224, 307, 362, 392, 398
NspI RCATGY 1 cut(s) 26
PaeI GCATGC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 5 cut(s) 105, 108, 117, 123, 240
PctI GAATGC 1 cut(s) 98
PkrI GCNGC 4 cut(s) 49, 86, 171, 382
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
Psp124BI GAGCTC 2 cut(s) 108, 147
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 393
SacI GAGCTC 2 cut(s) 108, 147
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 4 cut(s) 48, 85, 170, 381
Sau3AI GATC 1 cut(s) 300
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 2 cut(s) 108, 147
SetI ASST 5 cut(s) 108, 114, 147, 242, 291
SfaNI GCATC 2 cut(s) 214, 235
SgrAI CRCCGGYG 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SphI GCATGC 1 cut(s) 26
SsiI CCGC 3 cut(s) 48, 248, 380
SstI GAGCTC 2 cut(s) 108, 147
StyD4I CCNGG 1 cut(s) 172
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 1 cut(s) 114
TaqI TCGA 6 cut(s) 75, 99, 108, 134, 234, 243
TauI GCSGC 2 cut(s) 50, 383
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 16
TseI GCWGC 2 cut(s) 84, 169
TspDTI ATGAA 3 cut(s) 17, 405, 411
TspGWI ACGGA 1 cut(s) 137
TspRI CASTG 1 cut(s) 16
XceI RCATGY 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.