Rorug02G0637800

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0016114
Physical Location & Seq
Forward (+)
75094064 .. 75096580
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0637800.1

Sequence Viewer

Length: 1383 bp
ATGGGAATTCAAGCTCCAACCGACTATAGTTCTTCTGCCCCAAGCAATATGGATGAGCAACCGGAGAAGCAAGCAAAGATAGATGAACAACTACAGAGGCAGAAGGAAATTGATGATTGGCTTCCAATTACTTCATCAAGGAATGCAAAATGGTGGTATTCAGCTTTCCACAATGTCACTGCCATGGTTGGAGCTGGTGTCCTCAGTCTTCCCTTTGCCATGTCTAATCTTGGATGGGGTCCTGGTGTAGTTATTCTGATATTATCATGGGTGATCACTTTATTCACATTATGGCAAATGGTTGAGATGCATGAAATGGTTCCGGGAAAGCGGTTTGATAGGTACCATGAACTGGGTCAACATGCCTTTGGTGAAAAGCTTGGTCTTTGGATTGTGGTGCCCCAACAGCTGATCTGCGAAGTTGGCGTGGACATTGTGTACATGGTCACTGGAGGAAAATCGCTGCAGAAGGTCCATAACATAGTTCGTAAAGACGAGAAAGACTCAATCAAACTCAGTTACTTCATCATGATATTTGCCTCTGCTCATTTTGTACTTGCTCATCTCCCCAACTTCAACTCCATATCTGGGGTGTCTTTGGCAGCAGCAGTCATGTCATTAAGTTACTCTACTATTGCATGGACGGCTTCAATCCATAAGGGTGTTCAGGCAGATGTAGATTATGGTTATTTAGCCCACAGCACATCAGGAACAGTCTTTAACTTCTTCAGTGCCTTGGGCGATGTTGCTTTTGCCTATGCAGGCCACAATGTGGTTCTGGAGATCCAAGCAACACTCCCTTCCACACCTGAAACCCCATCGAAGAAGCCTATGTGGAGGGGTGTTGTGATAGCCTACATAGTTGTGGCTTTGTGCTATTTTCCAGTGGCTCTCATTGGATACTACACCTTTGGCAACAAAGTTGAGGACAACATCTTAATATCATTGGAGAAACCTGAATGGCTCATCATAATGGCAAACATGTTTGTTGTTGTCCATGTTATTGGGAGTTATCAGCTATATGCAATGCCAGTGTTTGACATGATAGAAACTGTCCTGGTAAAGAAACTAAAGTTCAGGCCAACTACAACTCTTCGCTTCATTTCACGCAATTTATATGTTGCATTCACAATGTTCGTCGGTATTACTTTTCCCTTCTTTGGTGGACTTCTGGGATTCTTTGGCGGATTTGCCTTTTCCCCAACAACATACTTTCTCCCTTGCATCATGTGGCTTGCCATCAAGAAACCGCGAAAGTTTAGCTTATCTTGGATCGTTAACTGGATTTGCATTACATTGGGAGTCATGCTCATGATCCTATCACCTATTGGAGGCTTAAGGAGTATCATACTTCAAGCCAAGAAGTACAAGTTCTACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

460

Amino Acids

51.67

Weight (kDa)

8.9

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 48 - 444 3e-110 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54530
fragaria_vesca FvH4_6g03460
malus_domestica MD04G1220600.v1.1
prunus_persica Prupe.6G339600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451951
rosa_laevigata RLG00000025634
rosa_multiflora Rmu_sc0006510.1_g000001
rosa_roxburghii Rroxscaffold_164G00436160 Rroxscaffold_6G00429130
rosa_rugosa Rorug02G0637800
rosa_samantha Rh3AG039400 Rh3BG040800 Rh3CG039300 Rh3DG040000
rosa_wichuraiana Rw3G002960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 342
AccB1I GGYRCC 2 cut(s) 342, 397
AccB7I CCANNNNNTGG 2 cut(s) 352, 772
AccII CGCG 1 cut(s) 1252
AciI CCGC 3 cut(s) 331, 1185, 1250
AclWI GGATC 3 cut(s) 778, 1280, 1309
AcsI RAATTY 1 cut(s) 6
AcuI CTGAAG 1 cut(s) 712
AdeI CACNNNGTG 1 cut(s) 772
AfaI GTAC 4 cut(s) 344, 440, 555, 1367
AfiI CCNNNNNNNGG 5 cut(s) 352, 588, 772, 1160, 1331
AflII CTTAAG 1 cut(s) 1336
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 4 cut(s) 11, 577, 651, 1355
AjnI CCWGG 2 cut(s) 241, 1056
AluBI AGCT 7 cut(s) 14, 164, 194, 379, 409, 1018, 1263
AluI AGCT 7 cut(s) 14, 164, 194, 379, 409, 1018, 1263
AlwI GGATC 3 cut(s) 778, 1280, 1309
AoxI GGCC 2 cut(s) 763, 1079
ApeKI GCWGC 3 cut(s) 463, 602, 605
ApoI RAATTY 1 cut(s) 6
Asp700I GAANNNNTTC 1 cut(s) 318
Asp718I GGTACC 1 cut(s) 342
AspS9I GGNCC 2 cut(s) 239, 472
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 3 cut(s) 283, 383, 1314
AvaII GGWCC 2 cut(s) 239, 472
BaeGI GKGCMC 1 cut(s) 402
BanI GGYRCC 2 cut(s) 342, 397
BbsI GAAGAC 1 cut(s) 200
BbvI GCAGC 3 cut(s) 450, 614, 617
BccI CCATC 3 cut(s) 228, 826, 1247
BceAI ACGGC 1 cut(s) 660
BciT130I CCWGG 2 cut(s) 243, 1058
BciVI GTATCC 1 cut(s) 893
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 1 cut(s) 324
BfmI CTRYAG 3 cut(s) 25, 92, 464
BfrI CTTAAG 1 cut(s) 1336
BfuI GTATCC 1 cut(s) 893
BisI GCNGC 3 cut(s) 464, 603, 606
BlsI GCNGC 3 cut(s) 465, 604, 607
Bme1390I CCNGG 3 cut(s) 243, 324, 1058
Bme18I GGWCC 2 cut(s) 239, 472
BmgT120I GGNCC 2 cut(s) 239, 472
BmiI GGNNCC 4 cut(s) 240, 321, 344, 399
BmrFI CCNGG 3 cut(s) 243, 324, 1058
BmrI ACTGGG 1 cut(s) 362
BmsI GCATC 2 cut(s) 297, 1233
BmuI ACTGGG 1 cut(s) 362
BpiI GAAGAC 1 cut(s) 200
BplI GAGNNNNNCTC 4 cut(s) 488, 520, 1293, 1325
BpmI CTGGAG 2 cut(s) 471, 800
BpuMI CCSGG 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 183, 735
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 4 cut(s) 183, 213, 563, 593
Bsc4I CCNNNNNNNGG 5 cut(s) 352, 588, 772, 1160, 1331
Bse1I ACTGG 5 cut(s) 357, 454, 884, 1031, 1286
Bse3DI GCAATG 1 cut(s) 1032
BseBI CCWGG 2 cut(s) 243, 1058
BseDI CCNNGG 2 cut(s) 183, 735
BseGI GGATG 2 cut(s) 58, 239
BseLI CCNNNNNNNGG 5 cut(s) 352, 588, 772, 1160, 1331
BseMI GCAATG 1 cut(s) 1032
BseMII CTCAG 2 cut(s) 217, 529
BseNI ACTGG 5 cut(s) 357, 454, 884, 1031, 1286
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 3 cut(s) 450, 614, 617
Bsh1236I CGCG 1 cut(s) 1252
BshFI GGCC 2 cut(s) 765, 1081
BshNI GGYRCC 2 cut(s) 342, 397
BsiSI CCGG 2 cut(s) 62, 323
BslI CCNNNNNNNGG 5 cut(s) 352, 588, 772, 1160, 1331
BsmI GAATGC 2 cut(s) 148, 1124
BsnI GGCC 2 cut(s) 765, 1081
Bsp1286I GDGCHC 1 cut(s) 402
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 5 cut(s) 273, 411, 783, 1272, 1314
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 3 cut(s) 331, 1185, 1250
BspANI GGCC 2 cut(s) 765, 1081
BspCNI CTCAG 2 cut(s) 216, 528
BspFNI CGCG 1 cut(s) 1252
BspHI TCATGA 2 cut(s) 528, 1311
BspLI GGNNCC 4 cut(s) 240, 321, 344, 399
BspMAI CTGCAG 1 cut(s) 468
BspPI GGATC 3 cut(s) 778, 1280, 1309
BspT107I GGYRCC 2 cut(s) 342, 397
BspTI CTTAAG 1 cut(s) 1336
BsrDI GCAATG 1 cut(s) 1032
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 5 cut(s) 357, 454, 884, 1031, 1286
BssECI CCNNGG 2 cut(s) 183, 735
BssMI GATC 5 cut(s) 273, 411, 783, 1272, 1314
BssT1I CCWWGG 2 cut(s) 183, 735
Bst2UI CCWGG 2 cut(s) 243, 1058
Bst4CI ACNGT 2 cut(s) 715, 1054
Bst6I CTCTTC 1 cut(s) 1098
BstAFI CTTAAG 1 cut(s) 1336
BstAUI TGTACA 1 cut(s) 438
BstC8I GCNNGC 3 cut(s) 72, 763, 1236
BstDEI CTNAG 2 cut(s) 203, 515
BstDSI CCRYGG 1 cut(s) 183
BstENI CCTNNNNNAGG 1 cut(s) 1329
BstF5I GGATG 2 cut(s) 58, 239
BstFNI CGCG 1 cut(s) 1252
BstKTI GATC 5 cut(s) 276, 414, 786, 1275, 1317
BstMBI GATC 5 cut(s) 273, 411, 783, 1272, 1314
BstMWI GCNNNNNNNGC 3 cut(s) 406, 423, 644
BstNI CCWGG 2 cut(s) 243, 1058
BstNSI RCATGY 2 cut(s) 365, 985
BstSCI CCNGG 3 cut(s) 241, 322, 1056
BstSFI CTRYAG 3 cut(s) 25, 92, 464
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 1 cut(s) 1252
BstV1I GCAGC 3 cut(s) 450, 614, 617
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 1 cut(s) 783
BstXI CCANNNNNNTGG 1 cut(s) 1004
BstYI RGATCY 1 cut(s) 783
BsuI GTATCC 1 cut(s) 893
BsuRI GGCC 2 cut(s) 765, 1081
BtgI CCRYGG 1 cut(s) 183
BtgZI GCGATG 1 cut(s) 756
BtsCI GGATG 2 cut(s) 58, 239
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 5 cut(s) 177, 447, 736, 891, 1038
Cac8I GCNNGC 3 cut(s) 72, 763, 1236
CciI TCATGA 2 cut(s) 528, 1311
Cfr13I GGNCC 2 cut(s) 239, 472
Csp6I GTAC 4 cut(s) 343, 439, 554, 1366
CviQI GTAC 4 cut(s) 343, 439, 554, 1366
DdeI CTNAG 2 cut(s) 203, 515
DpnI GATC 5 cut(s) 275, 413, 785, 1274, 1316
DpnII GATC 5 cut(s) 273, 411, 783, 1272, 1314
DraIII CACNNNGTG 1 cut(s) 772
Eam1104I CTCTTC 1 cut(s) 1098
EarI CTCTTC 1 cut(s) 1098
EciI GGCGGA 1 cut(s) 1200
Eco130I CCWWGG 2 cut(s) 183, 735
Eco47I GGWCC 2 cut(s) 239, 472
Eco57I CTGAAG 1 cut(s) 712
EcoNI CCTNNNNNAGG 1 cut(s) 1329
EcoO109I RGGNCCY 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 6
EcoRII CCWGG 2 cut(s) 241, 1056
EcoT14I CCWWGG 2 cut(s) 183, 735
EcoT22I ATGCAT 1 cut(s) 312
ErhI CCWWGG 2 cut(s) 183, 735
FalI AAGNNNNNCTT 2 cut(s) 1247, 1279
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 3 cut(s) 464, 603, 606
FokI GGATG 2 cut(s) 65, 246
Fsp4HI GCNGC 3 cut(s) 464, 603, 606
GluI GCNGC 3 cut(s) 464, 603, 606
GsuI CTGGAG 2 cut(s) 471, 800
HaeIII GGCC 2 cut(s) 765, 1081
HapII CCGG 2 cut(s) 62, 323
HincII GTYRAC 2 cut(s) 359, 1279
HindII GTYRAC 2 cut(s) 359, 1279
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 3 cut(s) 503, 1176, 1302
HpaI GTTAAC 1 cut(s) 1279
HpaII CCGG 2 cut(s) 62, 323
HphI GGTGA 3 cut(s) 283, 383, 1314
Hpy166II GTNNAC 5 cut(s) 359, 430, 439, 1166, 1279
Hpy188I TCNGA 1 cut(s) 258
Hpy188III TCNNGA 5 cut(s) 529, 708, 779, 1243, 1312
Hpy8I GTNNAC 5 cut(s) 359, 430, 439, 1166, 1279
Hpy99I CGWCG 1 cut(s) 1142
HpyAV CCTTC 4 cut(s) 97, 463, 810, 1165
HpyCH4III ACNGT 2 cut(s) 715, 1054
HpyCH4V TGCA 9 cut(s) 146, 310, 466, 638, 761, 1025, 1124, 1224, 1290
HpyF10VI GCNNNNNNNGC 3 cut(s) 406, 423, 644
HpyF3I CTNAG 2 cut(s) 203, 515
KpnI GGTACC 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 273
KspAI GTTAAC 1 cut(s) 1279
Kzo9I GATC 5 cut(s) 273, 411, 783, 1272, 1314
LmnI GCTCC 2 cut(s) 19, 191
Lsp1109I GCAGC 3 cut(s) 450, 614, 617
LweI GCATC 2 cut(s) 297, 1233
MaeIII GTNAC 4 cut(s) 175, 445, 518, 623
MalI GATC 5 cut(s) 275, 413, 785, 1274, 1316
MboI GATC 5 cut(s) 273, 411, 783, 1272, 1314
MboII GAAGA 5 cut(s) 24, 200, 718, 835, 1085
MfeI CAATTG 1 cut(s) 1378
MflI RGATCY 1 cut(s) 783
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 5 cut(s) 6, 108, 126, 1111, 1378
MlyI GAGTC 2 cut(s) 497, 1311
MmeI TCCRAC 2 cut(s) 41, 169
MnlI CCTC 7 cut(s) 90, 212, 446, 550, 831, 919, 1325
Mph1103I ATGCAT 1 cut(s) 312
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 5 cut(s) 620, 720, 938, 1278, 1337
MslI CAYNNNNRTG 5 cut(s) 182, 660, 863, 971, 1310
MspA1I CMGCKG 1 cut(s) 409
MspCI CTTAAG 1 cut(s) 1336
MspI CCGG 2 cut(s) 62, 323
MspR9I CCNGG 3 cut(s) 243, 324, 1058
MunI CAATTG 1 cut(s) 1378
Mva1269I GAATGC 2 cut(s) 148, 1124
MvaI CCWGG 2 cut(s) 243, 1058
MvnI CGCG 1 cut(s) 1252
MwoI GCNNNNNNNGC 3 cut(s) 406, 423, 644
NciI CCSGG 1 cut(s) 324
NcoI CCATGG 1 cut(s) 183
NdeII GATC 5 cut(s) 273, 411, 783, 1272, 1314
NlaIV GGNNCC 4 cut(s) 240, 321, 344, 399
NmuCI GTSAC 2 cut(s) 175, 445
NsiI ATGCAT 1 cut(s) 312
NspI RCATGY 2 cut(s) 365, 985
PagI TCATGA 2 cut(s) 528, 1311
PciI ACATGT 1 cut(s) 981
PctI GAATGC 2 cut(s) 148, 1124
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 1 cut(s) 1176
PflMI CCANNNNNTGG 2 cut(s) 352, 772
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 3 cut(s) 465, 604, 607
PleI GAGTC 2 cut(s) 497, 1310
PpsI GAGTC 2 cut(s) 497, 1310
PpuMI RGGWCCY 1 cut(s) 239
PscI ACATGT 1 cut(s) 981
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 241, 1056
PspGI CCWGG 2 cut(s) 241, 1056
PspN4I GGNNCC 4 cut(s) 240, 321, 344, 399
PspPI GGNCC 2 cut(s) 239, 472
PspPPI RGGWCCY 1 cut(s) 239
PstI CTGCAG 1 cut(s) 468
PsuI RGATCY 1 cut(s) 783
PvuII CAGCTG 1 cut(s) 409
RsaI GTAC 4 cut(s) 344, 440, 555, 1367
RsaNI GTAC 4 cut(s) 343, 439, 554, 1366
RseI CAYNNNNRTG 5 cut(s) 182, 660, 863, 971, 1310
SaqAI TTAA 5 cut(s) 620, 720, 938, 1278, 1337
SatI GCNGC 3 cut(s) 464, 603, 606
Sau3AI GATC 5 cut(s) 273, 411, 783, 1272, 1314
Sau96I GGNCC 2 cut(s) 239, 472
SchI GAGTC 2 cut(s) 497, 1311
ScrFI CCNGG 3 cut(s) 243, 324, 1058
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 2 cut(s) 297, 1233
SfcI CTRYAG 3 cut(s) 25, 92, 464
SinI GGWCC 2 cut(s) 239, 472
SmiMI CAYNNNNRTG 5 cut(s) 182, 660, 863, 971, 1310
SmlI CTYRAG 1 cut(s) 1336
SmoI CTYRAG 1 cut(s) 1336
Sse9I AATT 5 cut(s) 6, 108, 126, 1111, 1378
SsiI CCGC 3 cut(s) 331, 1185, 1250
StyD4I CCNGG 3 cut(s) 241, 322, 1056
StyI CCWWGG 2 cut(s) 183, 735
TaaI ACNGT 2 cut(s) 715, 1054
TaqI TCGA 1 cut(s) 821
TasI AATT 5 cut(s) 6, 108, 126, 1111, 1378
TatI WGTACW 3 cut(s) 438, 553, 1365
TfiI GAWTC 1 cut(s) 1176
Tru1I TTAA 5 cut(s) 620, 720, 938, 1278, 1337
Tru9I TTAA 5 cut(s) 620, 720, 938, 1278, 1337
TscAI CASTG 5 cut(s) 184, 454, 736, 891, 1038
TseFI GTSAC 2 cut(s) 175, 445
TseI GCWGC 3 cut(s) 463, 602, 605
Tsp45I GTSAC 2 cut(s) 175, 445
TspDTI ATGAA 6 cut(s) 99, 123, 327, 363, 514, 1090
TspRI CASTG 5 cut(s) 184, 454, 736, 891, 1038
Van91I CCANNNNNTGG 2 cut(s) 352, 772
Vha464I CTTAAG 1 cut(s) 1336
VpaK11BI GGWCC 2 cut(s) 239, 472
XagI CCTNNNNNAGG 1 cut(s) 1329
XapI RAATTY 1 cut(s) 6
XceI RCATGY 2 cut(s) 365, 985
XmnI GAANNNNTTC 1 cut(s) 318
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.