MD04G1220600.v1.1

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016114
Physical Location & Seq
Forward (+)
30183047 .. 30183475
429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1220600.v1.1.491

Sequence Viewer

Length: 429 bp
ATGAAAACGAGTAGCAGTTCCAGTGCCGTTGTTATTCATGCCCTGAACAATTTGACCGTCACCGGATTCGTGGAAGACACAACGATGTTTGAGAAATGCAGTAAGGAATGCTTCGGAAAACTTGACGTGGACGGTCAGGGAGGGCTATCGAGGGAGAAGCTACGTGCAGGGTTTGGCAAACTTTTGCCGGGCATCGGGTACGTATCGCAGCCGAAAGACGAAATCAACGTCCTGCATGATGAAATATTTGAGAGATTTGATGCGGATAAAAATGGTGTGATCGATGGTCAAGAGTTTCAGACTCTGTTGGCGGAGACGATGCTCGCGGTGGCGCGAGGGATCGGGGGTTCGCCGGTTCTGGTGGCGCTTGAACATGGCAGCCTCCTCATGAGGGCTGCTGAACATGAAAAGGCAAGAGTATGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

143

Amino Acids

15.33

Weight (kDa)

5.55

Isoelectric Point (pI)

25.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 35 - 102 1.7e-07 EF-hand domain pair
EF-hand_1 PF00036 80 - 105 5.2e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54530
fragaria_vesca FvH4_6g03460
malus_domestica MD04G1220600.v1.1
prunus_persica Prupe.6G339600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451951
rosa_laevigata RLG00000025634
rosa_multiflora Rmu_sc0006510.1_g000001
rosa_roxburghii Rroxscaffold_164G00436160 Rroxscaffold_6G00429130
rosa_rugosa Rorug02G0637800
rosa_samantha Rh3AG039400 Rh3BG040800 Rh3CG039300 Rh3DG040000
rosa_wichuraiana Rw3G002960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 326, 334
AciI CCGC 3 cut(s) 263, 311, 326
AclWI GGATC 1 cut(s) 347
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 194, 391
AgsI TTSAA 1 cut(s) 371
AjiI CACGTC 1 cut(s) 127
AloI GAACNNNNNNTCC 2 cut(s) 331, 363
AluBI AGCT 1 cut(s) 160
AluI AGCT 1 cut(s) 160
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 1 cut(s) 347
AlwNI CAGNNNCTG 1 cut(s) 304
ApeKI GCWGC 3 cut(s) 208, 378, 395
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
AspLEI GCGC 2 cut(s) 334, 367
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 81
BbvI GCAGC 3 cut(s) 220, 382, 390
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 11
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 308
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 3 cut(s) 209, 379, 396
BlsI GCNGC 3 cut(s) 210, 380, 397
Bme1390I CCNGG 1 cut(s) 189
BmgBI CACGTC 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 3 cut(s) 201, 250, 309
BpiI GAAGAC 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 189
Bsa29I ATCGAT 1 cut(s) 282
BsaAI YACGTR 2 cut(s) 164, 202
BsaWI WCCGGW 1 cut(s) 62
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 391
Bse118I RCCGGY 1 cut(s) 352
Bse1I ACTGG 1 cut(s) 21
BseCI ATCGAT 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 194, 391
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 3 cut(s) 220, 382, 390
BsgI GTGCAG 1 cut(s) 186
Bsh1236I CGCG 2 cut(s) 326, 334
BshVI ATCGAT 1 cut(s) 282
BsiSI CCGG 3 cut(s) 63, 188, 353
BslI CCNNNNNNNGG 2 cut(s) 194, 391
BsmAI GTCTC 1 cut(s) 308
BsmBI CGTCTC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 113
Bsp143I GATC 2 cut(s) 279, 339
BspACI CCGC 3 cut(s) 263, 311, 326
BspDI ATCGAT 1 cut(s) 282
BspFNI CGCG 2 cut(s) 326, 334
BspHI TCATGA 1 cut(s) 387
BspPI GGATC 1 cut(s) 347
BsrFI RCCGGY 1 cut(s) 352
BsrI ACTGG 1 cut(s) 21
BssAI RCCGGY 1 cut(s) 352
BssMI GATC 2 cut(s) 279, 339
Bst4CI ACNGT 2 cut(s) 58, 134
BstBAI YACGTR 2 cut(s) 164, 202
BstC8I GCNNGC 1 cut(s) 324
BstFNI CGCG 2 cut(s) 326, 334
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 2 cut(s) 334, 367
BstKTI GATC 2 cut(s) 282, 342
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 2 cut(s) 279, 339
BstSCI CCNGG 1 cut(s) 187
BstSNI TACGTA 1 cut(s) 202
BstUI CGCG 2 cut(s) 326, 334
BstV1I GCAGC 3 cut(s) 220, 382, 390
BstV2I GAAGAC 1 cut(s) 81
Bsu15I ATCGAT 1 cut(s) 282
BsuTUI ATCGAT 1 cut(s) 282
BtrI CACGTC 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 28
Cac8I GCNNGC 1 cut(s) 324
CaiI CAGNNNCTG 1 cut(s) 304
CciI TCATGA 1 cut(s) 387
CfoI GCGC 2 cut(s) 334, 367
Cfr10I RCCGGY 1 cut(s) 352
ClaI ATCGAT 1 cut(s) 282
Csp6I GTAC 1 cut(s) 199
CviAII CATG 5 cut(s) 38, 236, 374, 388, 404
CviJI RGCY 5 cut(s) 145, 160, 211, 381, 395
CviKI_1 RGCY 5 cut(s) 145, 160, 211, 381, 395
CviQI GTAC 1 cut(s) 199
DpnI GATC 2 cut(s) 281, 341
DpnII GATC 2 cut(s) 279, 339
EciI GGCGGA 1 cut(s) 326
Eco105I TACGTA 1 cut(s) 202
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 5 cut(s) 41, 239, 377, 391, 407
FaiI YATR 6 cut(s) 39, 237, 375, 389, 405, 421
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FatI CATG 5 cut(s) 37, 235, 373, 387, 403
Fnu4HI GCNGC 3 cut(s) 209, 379, 396
Fsp4HI GCNGC 3 cut(s) 209, 379, 396
GlaI GCGC 2 cut(s) 333, 366
GluI GCNGC 3 cut(s) 209, 379, 396
HaeII RGCGCY 1 cut(s) 368
HapII CCGG 3 cut(s) 63, 188, 353
HhaI GCGC 2 cut(s) 334, 367
Hin1II CATG 5 cut(s) 41, 239, 377, 391, 407
Hin6I GCGC 2 cut(s) 332, 365
HinP1I GCGC 2 cut(s) 332, 365
HinfI GANTC 2 cut(s) 66, 301
HpaII CCGG 3 cut(s) 63, 188, 353
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 2 cut(s) 116, 300
Hpy188III TCNNGA 2 cut(s) 290, 388
Hpy8I GTNNAC 1 cut(s) 130
HpyCH4III ACNGT 2 cut(s) 58, 134
HpyCH4IV ACGT 4 cut(s) 126, 163, 201, 228
HpyCH4V TGCA 4 cut(s) 99, 167, 235, 423
HpySE526I ACGT 4 cut(s) 126, 163, 201, 228
Hsp92II CATG 5 cut(s) 41, 239, 377, 391, 407
HspAI GCGC 2 cut(s) 332, 365
Kzo9I GATC 2 cut(s) 279, 339
LpnPI CCDG 9 cut(s) 34, 56, 76, 122, 153, 201, 245, 344, 366
Lsp1109I GCAGC 3 cut(s) 220, 382, 390
LweI GCATC 3 cut(s) 201, 250, 309
MaeII ACGT 4 cut(s) 126, 163, 201, 228
MaeIII GTNAC 1 cut(s) 58
MalI GATC 2 cut(s) 281, 341
MboI GATC 2 cut(s) 279, 339
MboII GAAGA 1 cut(s) 86
MluCI AATT 1 cut(s) 49
MlyI GAGTC 1 cut(s) 295
MnlI CCTC 6 cut(s) 134, 144, 329, 384, 392, 395
MslI CAYNNNNRTG 1 cut(s) 83
MspI CCGG 3 cut(s) 63, 188, 353
MspR9I CCNGG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 113
MvnI CGCG 2 cut(s) 326, 334
NciI CCSGG 1 cut(s) 189
NdeII GATC 2 cut(s) 279, 339
NlaIII CATG 5 cut(s) 41, 239, 377, 391, 407
NmuCI GTSAC 1 cut(s) 58
PagI TCATGA 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 66
PkrI GCNGC 3 cut(s) 210, 380, 397
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Ppu21I YACGTR 2 cut(s) 164, 202
PstNI CAGNNNCTG 1 cut(s) 304
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 83
SatI GCNGC 3 cut(s) 209, 379, 396
Sau3AI GATC 2 cut(s) 279, 339
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 1 cut(s) 189
SetI ASST 5 cut(s) 129, 162, 166, 204, 231
SfaNI GCATC 3 cut(s) 201, 250, 309
SmiMI CAYNNNNRTG 1 cut(s) 83
SnaBI TACGTA 1 cut(s) 202
Sse9I AATT 1 cut(s) 49
SsiI CCGC 3 cut(s) 263, 311, 326
SspI AATATT 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 187
TaaI ACNGT 2 cut(s) 58, 134
TaiI ACGT 4 cut(s) 129, 166, 204, 231
TaqI TCGA 2 cut(s) 149, 282
TasI AATT 1 cut(s) 49
TfiI GAWTC 1 cut(s) 66
TscAI CASTG 1 cut(s) 28
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 3 cut(s) 208, 378, 395
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 4 cut(s) 17, 26, 255, 420
TspRI CASTG 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.