RLG00000025634

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016114
Physical Location & Seq
Forward (+)
47226319 .. 47226729
411 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025634

Sequence Viewer

Length: 411 bp
ATGAAGATCACCAGCGTTGTTGTGCATGCCCTAAACGACTCCACGGTGGCCGGATTCGTGGACGACACGACGCCCTTCGAGCAATGCAGCAAGGAATGCTTCGACGAGCTTGACCTTAACGCCGACGGCGTGTTGTCGAAGGAGGAGCTTCGTTCGGGTTTTGGTAAGCTGCTGCCTGGCATAGGGTACGTGTCACAGCCCAAAGAGGAGTTGAATGGTTTGCATGATGAAATATTTGAGAGGTTCGATGCGGATCAAAACGGCGTCGTTGACCGGGGTGAGTTTAAAGAGTTGTTGAGGGAGATCATGCTCGGGGTGGCGCGTGGGATTGGGGGGTCGCCGGTGCTGGTGGCGCTTGAGCATGGGAGTCTGCTTATGAGGGCAGCTCAACACGAACAGAATAAGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

137

Amino Acids

14.84

Weight (kDa)

4.68

Isoelectric Point (pI)

28.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_5 PF13202 31 - 52 5.6e-06 EF hand
EF-hand_7 PF13499 31 - 99 1.7e-09 EF-hand domain pair
EF-hand_1 PF00036 76 - 101 1.9e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54530
fragaria_vesca FvH4_6g03460
malus_domestica MD04G1220600.v1.1
prunus_persica Prupe.6G339600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451951
rosa_laevigata RLG00000025634
rosa_multiflora Rmu_sc0006510.1_g000001
rosa_roxburghii Rroxscaffold_164G00436160 Rroxscaffold_6G00429130
rosa_rugosa Rorug02G0637800
rosa_samantha Rh3AG039400 Rh3BG040800 Rh3CG039300 Rh3DG040000
rosa_wichuraiana Rw3G002960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 322
AciI CCGC 1 cut(s) 251
AclWI GGATC 1 cut(s) 261
AcoI YGGCCR 1 cut(s) 48
AcyI GRCGYC 2 cut(s) 71, 264
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 1 cut(s) 182
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 1 cut(s) 175
AluBI AGCT 4 cut(s) 109, 148, 169, 386
AluI AGCT 4 cut(s) 109, 148, 169, 386
AlwI GGATC 1 cut(s) 261
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 4 cut(s) 87, 169, 172, 383
AspLEI GCGC 2 cut(s) 322, 355
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 1 cut(s) 290
AvaI CYCGRG 1 cut(s) 311
BbvI GCAGC 4 cut(s) 99, 156, 159, 395
BceAI ACGGC 2 cut(s) 142, 277
BciT130I CCWGG 1 cut(s) 177
BcnI CCSGG 1 cut(s) 275
BfoI RGCGCY 1 cut(s) 356
BisI GCNGC 4 cut(s) 88, 170, 173, 384
BlsI GCNGC 4 cut(s) 89, 171, 174, 385
Bme1390I CCNGG 2 cut(s) 177, 275
BmeT110I CYCGRG 1 cut(s) 311
BmrFI CCNGG 2 cut(s) 177, 275
BmsI GCATC 1 cut(s) 238
BplI GAGNNNNNCTC 2 cut(s) 370, 402
BpuEI CTTGAG 1 cut(s) 377
BpuMI CCSGG 1 cut(s) 275
BsaAI YACGTR 1 cut(s) 190
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 2 cut(s) 71, 264
BsaJI CCNNGG 2 cut(s) 42, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse118I RCCGGY 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 89
Bse8I GATNNNNATC 1 cut(s) 252
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 2 cut(s) 42, 274
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMI GCAATG 1 cut(s) 89
BseRI GAGGAG 2 cut(s) 158, 221
BseXI GCAGC 4 cut(s) 99, 156, 159, 395
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 1 cut(s) 50
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 3 cut(s) 51, 274, 341
BslI CCNNNNNNNGG 1 cut(s) 182
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 311
Bsp143I GATC 3 cut(s) 6, 253, 303
BspACI CCGC 1 cut(s) 251
BspANI GGCC 1 cut(s) 50
BspFNI CGCG 1 cut(s) 322
BspPI GGATC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 340
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 2 cut(s) 42, 274
BssMI GATC 3 cut(s) 6, 253, 303
BssNI GRCGYC 2 cut(s) 71, 264
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 1 cut(s) 46
BstACI GRCGYC 2 cut(s) 71, 264
BstAPI GCANNNNNTGC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 42
BstENI CCTNNNNNAGG 1 cut(s) 180
BstFNI CGCG 1 cut(s) 322
BstH2I RGCGCY 1 cut(s) 356
BstHHI GCGC 2 cut(s) 322, 355
BstKTI GATC 3 cut(s) 9, 256, 306
BstMBI GATC 3 cut(s) 6, 253, 303
BstMWI GCNNNNNNNGC 3 cut(s) 79, 96, 352
BstNI CCWGG 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 2 cut(s) 175, 273
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 4 cut(s) 99, 156, 159, 395
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 42
Cac8I GCNNGC 1 cut(s) 27
CfoI GCGC 2 cut(s) 322, 355
Cfr10I RCCGGY 1 cut(s) 340
CseI GACGC 2 cut(s) 79, 253
Csp6I GTAC 1 cut(s) 187
CviAII CATG 4 cut(s) 26, 224, 307, 362
CviJI RGCY 6 cut(s) 50, 109, 148, 169, 199, 386
CviKI_1 RGCY 6 cut(s) 50, 109, 148, 169, 199, 386
CviQI GTAC 1 cut(s) 187
DpnI GATC 3 cut(s) 8, 255, 305
DpnII GATC 3 cut(s) 6, 253, 303
DraI TTTAAA 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 311
EcoNI CCTNNNNNAGG 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 175
FaeI CATG 4 cut(s) 29, 227, 310, 365
FaiI YATR 6 cut(s) 27, 182, 225, 308, 363, 377
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FatI CATG 4 cut(s) 25, 223, 306, 361
Fnu4HI GCNGC 4 cut(s) 88, 170, 173, 384
Fsp4HI GCNGC 4 cut(s) 88, 170, 173, 384
GlaI GCGC 2 cut(s) 321, 354
GluI GCNGC 4 cut(s) 88, 170, 173, 384
HaeII RGCGCY 1 cut(s) 356
HaeIII GGCC 1 cut(s) 50
HapII CCGG 3 cut(s) 51, 274, 341
HgaI GACGC 2 cut(s) 79, 253
HhaI GCGC 2 cut(s) 322, 355
Hin1I GRCGYC 2 cut(s) 71, 264
Hin1II CATG 4 cut(s) 29, 227, 310, 365
Hin6I GCGC 2 cut(s) 320, 353
HinP1I GCGC 2 cut(s) 320, 353
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 3 cut(s) 38, 54, 367
HpaII CCGG 3 cut(s) 51, 274, 341
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 2 cut(s) 61, 271
Hpy8I GTNNAC 2 cut(s) 61, 271
Hpy99I CGWCG 4 cut(s) 73, 107, 128, 269
HpyAV CCTTC 2 cut(s) 85, 133
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 3 cut(s) 25, 87, 223
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 96, 352
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 2 cut(s) 71, 264
Hsp92II CATG 4 cut(s) 29, 227, 310, 365
HspAI GCGC 2 cut(s) 320, 353
Kzo9I GATC 3 cut(s) 6, 253, 303
LmnI GCTCC 1 cut(s) 145
LpnPI CCDG 7 cut(s) 25, 64, 162, 189, 287, 332, 354
Lsp1109I GCAGC 4 cut(s) 99, 156, 159, 395
LweI GCATC 1 cut(s) 238
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 192
MalI GATC 3 cut(s) 8, 255, 305
MboI GATC 3 cut(s) 6, 253, 303
MboII GAAGA 1 cut(s) 16
MlyI GAGTC 2 cut(s) 32, 376
MnlI CCTC 5 cut(s) 136, 199, 234, 291, 372
MseI TTAA 2 cut(s) 117, 285
MspI CCGG 3 cut(s) 51, 274, 341
MspR9I CCNGG 2 cut(s) 177, 275
Mva1269I GAATGC 1 cut(s) 101
MvaI CCWGG 1 cut(s) 177
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 3 cut(s) 79, 96, 352
NciI CCSGG 1 cut(s) 275
NdeII GATC 3 cut(s) 6, 253, 303
NlaIII CATG 4 cut(s) 29, 227, 310, 365
NmuCI GTSAC 1 cut(s) 192
NspI RCATGY 1 cut(s) 29
PaeI GCATGC 1 cut(s) 29
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 54
PkrI GCNGC 4 cut(s) 89, 171, 174, 385
PleI GAGTC 2 cut(s) 32, 375
PpsI GAGTC 2 cut(s) 32, 375
Ppu21I YACGTR 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SaqAI TTAA 2 cut(s) 117, 285
SatI GCNGC 4 cut(s) 88, 170, 173, 384
Sau3AI GATC 3 cut(s) 6, 253, 303
SchI GAGTC 2 cut(s) 32, 376
ScrFI CCNGG 2 cut(s) 177, 275
SetI ASST 7 cut(s) 111, 117, 150, 171, 192, 245, 388
SfaNI GCATC 1 cut(s) 238
SgrAI CRCCGGYG 1 cut(s) 340
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SphI GCATGC 1 cut(s) 29
SsiI CCGC 1 cut(s) 251
SspI AATATT 1 cut(s) 234
StyD4I CCNGG 2 cut(s) 175, 273
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 1 cut(s) 192
TaqI TCGA 4 cut(s) 78, 102, 137, 246
TfiI GAWTC 1 cut(s) 54
Tru1I TTAA 2 cut(s) 117, 285
Tru9I TTAA 2 cut(s) 117, 285
TseFI GTSAC 1 cut(s) 192
TseI GCWGC 4 cut(s) 87, 169, 172, 383
Tsp45I GTSAC 1 cut(s) 192
TspDTI ATGAA 2 cut(s) 17, 243
XagI CCTNNNNNAGG 1 cut(s) 180
XceI RCATGY 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.