Rh3AG039400

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0016114
Physical Location & Seq
Reverse (-)
2617048 .. 2618451
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG039400.1

Sequence Viewer

Length: 258 bp
ATGAAGGTCACCAGCGTTGTTGTGCATGCCCTAAACGACTCCACGGTGGCCGGATTCGTGGACGACACGACGCCTTTCGAGCAATGCAGCAAGGAATGCTTCGACGAGCTAGACCTTAACGCCGACGGCGTGTTGTCGAAGGAGGAGCTTCGTTCGGGTTTTGGTAAGCTGCTGCCGGGCATCGGGGATGTGCTGGGAATGTGCCCAGCAGCATTGAACCCAAGAGAGCTTGCCTCAAAAATCTGCAAGCCACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

85

Amino Acids

9.08

Weight (kDa)

4.77

Isoelectric Point (pI)

27.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_5 PF13202 31 - 52 2.5e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54530
fragaria_vesca FvH4_6g03460
malus_domestica MD04G1220600.v1.1
prunus_persica Prupe.6G339600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451951
rosa_laevigata RLG00000025634
rosa_multiflora Rmu_sc0006510.1_g000001
rosa_roxburghii Rroxscaffold_164G00436160 Rroxscaffold_6G00429130
rosa_rugosa Rorug02G0637800
rosa_samantha Rh3AG039400 Rh3BG040800 Rh3CG039300 Rh3DG040000
rosa_wichuraiana Rw3G002960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 182
AgsI TTSAA 1 cut(s) 217
AluBI AGCT 4 cut(s) 109, 148, 169, 229
AluI AGCT 4 cut(s) 109, 148, 169, 229
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 4 cut(s) 87, 169, 172, 209
AsuC2I CCSGG 1 cut(s) 177
BaeGI GKGCMC 1 cut(s) 206
BbvI GCAGC 4 cut(s) 99, 156, 159, 221
BceAI ACGGC 1 cut(s) 142
BcnI CCSGG 1 cut(s) 177
BfaI CTAG 2 cut(s) 110, 256
BisI GCNGC 4 cut(s) 88, 170, 173, 210
BlsI GCNGC 4 cut(s) 89, 171, 174, 211
Bme1390I CCNGG 1 cut(s) 177
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 189
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpuMI CCSGG 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 42
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse3DI GCAATG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 42
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMI GCAATG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 4 cut(s) 99, 156, 159, 221
BseYI CCCAGC 2 cut(s) 193, 205
BshFI GGCC 1 cut(s) 50
BsiSI CCGG 2 cut(s) 51, 176
BslI CCNNNNNNNGG 1 cut(s) 182
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 206
BspANI GGCC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 42
BssNI GRCGYC 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 46
BstACI GRCGYC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 96
BstC8I GCNNGC 3 cut(s) 27, 231, 248
BstDSI CCRYGG 1 cut(s) 42
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 1 cut(s) 193
BstMWI GCNNNNNNNGC 2 cut(s) 79, 96
BstNSI RCATGY 1 cut(s) 29
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 1 cut(s) 175
BstSLI GKGCMC 1 cut(s) 206
BstV1I GCAGC 4 cut(s) 99, 156, 159, 221
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 193
Cac8I GCNNGC 3 cut(s) 27, 231, 248
CseI GACGC 1 cut(s) 79
CviAII CATG 1 cut(s) 26
CviJI RGCY 6 cut(s) 50, 109, 148, 169, 229, 250
CviKI_1 RGCY 6 cut(s) 50, 109, 148, 169, 229, 250
EaeI YGGCCR 1 cut(s) 48
Eco91I GGTNACC 1 cut(s) 7
EcoO65I GGTNACC 1 cut(s) 7
FaeI CATG 1 cut(s) 29
FaiI YATR 1 cut(s) 27
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FatI CATG 1 cut(s) 25
Fnu4HI GCNGC 4 cut(s) 88, 170, 173, 210
FokI GGATG 1 cut(s) 200
Fsp4HI GCNGC 4 cut(s) 88, 170, 173, 210
FspBI CTAG 2 cut(s) 110, 256
GluI GCNGC 4 cut(s) 88, 170, 173, 210
GsaI CCCAGC 2 cut(s) 197, 209
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 51, 176
HgaI GACGC 1 cut(s) 79
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 1 cut(s) 29
HinfI GANTC 2 cut(s) 38, 54
HpaII CCGG 2 cut(s) 51, 176
Hpy166II GTNNAC 1 cut(s) 61
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 3 cut(s) 73, 107, 128
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4V TGCA 3 cut(s) 25, 87, 246
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 96
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 1 cut(s) 29
LmnI GCTCC 1 cut(s) 145
LpnPI CCDG 5 cut(s) 25, 64, 179, 189, 219
Lsp1109I GCAGC 4 cut(s) 99, 156, 159, 221
LweI GCATC 1 cut(s) 189
MaeI CTAG 2 cut(s) 110, 256
MaeIII GTNAC 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 206
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 2 cut(s) 136, 244
MseI TTAA 1 cut(s) 117
MspI CCGG 2 cut(s) 51, 176
MspR9I CCNGG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 101
MwoI GCNNNNNNNGC 2 cut(s) 79, 96
NciI CCSGG 1 cut(s) 177
NlaIII CATG 1 cut(s) 29
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 29
PaeI GCATGC 1 cut(s) 29
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 54
PkrI GCNGC 4 cut(s) 89, 171, 174, 211
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PspEI GGTNACC 1 cut(s) 7
PspFI CCCAGC 2 cut(s) 193, 205
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 4 cut(s) 88, 170, 173, 210
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 1 cut(s) 206
SetI ASST 6 cut(s) 9, 111, 117, 150, 171, 231
SfaNI GCATC 1 cut(s) 189
SphI GCATGC 1 cut(s) 29
SspMI CTAG 2 cut(s) 110, 256
StyD4I CCNGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 46
TaqI TCGA 3 cut(s) 78, 102, 137
TfiI GAWTC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 4 cut(s) 87, 169, 172, 209
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 1 cut(s) 17
XceI RCATGY 1 cut(s) 29
XspI CTAG 2 cut(s) 110, 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.