Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0014881

Physical Location & Seq

Genomic Coordinates Reverse (-)

4144228 .. 4146002

Gene Structure 1775 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g06871.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1572 bp

ATGCTTATGCAAACTTGCACCCAAATTGAAAACCAAAGTTCATGCCTCACAAAAGTGCAAGCTGAGCTCCAAAGCATAATGGAGCCTGATCAAGGTCATCAAGACGCTGCTTCTTCAAGAAGCTCAATCCTTAGTGCTGCGCTTAGGCACACACTGAATCAAGCAAAATCTGCCGTTGACATGATCACAAAGTTCAATGCTCTATCAATCAGTTACAGAGAACAAGTAGCTATTGAGGATTGCAAGGAGCTCCTTGATTTTTCTATCTCTGAGTTGGCTTGGTCTTTAGGCGTGATGAACAGAATTCGGACAGGTGACAGAAATGTTCACTATGAAGGAGACTTAAAAGCTTGGCTGAGTGCTGCCCTTAGTAACCAAGATACATGCCTTGAAGGTTTTGAGGGAACTGATAGACGTCTCGAAAACTTCATAAAGGGAAGCTTGAAGCAAGTCACACAGCTCATTGGTAATGTCTTGGCCTTGTACACTCAATTACATGATTTACCCTTTAAACCTCCAAGGGATCAGAGTATGAATACTTTGACAATTGCGAATAAGACTTCATCGCCTGATCATAATTTCCCGGCATGGATGAGTGAGGGTGATCAAGAGCTTGTTAAGTCTAGTCCACATGATCAATCAGGTATGCATGTAGATTCTGTTGTGGCAGCAGATGGGACTGGGAAGTACCGTTCAATCACAGAAGCTGTTAATGAAGCTCCAAACTACAGCAGTAGGAGGTACATAATATATGTGAAGAAGGGGATTTATAGAGAAAACATTGACATGAAGAAGAAGAAAACCAATATCATGTTTGTAGGGGATGGGATTGGACAAACTATAGTAACTGGTGACCGAAATTTCATGCAAGGATGGACTACATTTAGAACTGCAACAGTTGCTGTATCAGGAAAGGGATTTATAGCAAGAGACATGACTTTTCGCAACACAGCCGGGCCTCAAAACTATCAAGGCGTGGCACTTAGAGTTGACTCCGACCAATCTGCCTTCTTCCGGTGCAGCATGGAGGGTCACCAAGACACACTCTATGCTCACTCTCTTCGCCAATTTTACCGTGAATGTGACATCTATGGCACCATAGACTTCATATTTGGCAATGGCGCCGCGGTGCTCCAAAACTGCAAAATCTACACTCGAGTCCCTCTACCACTACAAAAGGTCACCATCACGGCTCAGGGTCGGAAAAGTCCGCATCAAAACACCGGGTTTTCAATCCAAGATAGTTATGTTTTTGCTACTCAACCAACATATCTTGGGAGGCCATGGAAGCTTTACTCTAGGACAGTTTTTATGAACACTTACATGAGTGGTCTAGTGCAGCCTAGAGGGTGGCTTGAGTGGTACGGTAACTTTGCTTTGGGAACATTATGGTACGGCGAGTATAGAAATCACGGGCCAGGTGCATCACTATCCGGCCGGGTCAAGTGGCCGGGATACCACATCATCCAGGATGCCAACATGGCTAGTTTCTTCACTGTTCGGCGATTTATCGATGGAATGGCTTGGTTGCCGGCCACTGGCGTTAAGTTTACAGTAGGTTTGAGTAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

524

Amino Acids

58.73

Weight (kDa)

8.76

Isoelectric Point (pI)

40.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	4 - 161	9.4e-26	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	220 - 506	1.3e-137	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05620
fragaria_vesca	FvH4_6g06871
malus_domestica	MD04G1198000.v1.1 MD12G1210600.v1.1
prunus_persica	Prupe.6G318500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0456351
rosa_laevigata	RLG00000025310
rosa_multiflora	Rmu_sc0002607.1_g000020 Rmu_sc0018054.1_g000002
rosa_roxburghii	Rroxscaffold_6G00422790
rosa_rugosa	Rorug03G0012700
rosa_samantha	Rh3AG072800 Rh3BG074900 Rh3CG074500 Rh3DG076300
rosa_wichuraiana	Rw3G006250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	418
AccB1I	GGYRCC	2 cut(s)	1094, 1121
AccII	CGCG	1 cut(s)	1127
AciI	CCGC	3 cut(s)	1125, 1127, 1211
AclWI	GGATC	1 cut(s)	531
AcoI	YGGCCR	3 cut(s)	1435, 1448, 1533
AcsI	RAATTY	2 cut(s)	303, 859
AcyI	GRCGYC	2 cut(s)	415, 1122
AfaI	GTAC	5 cut(s)	485, 689, 743, 1364, 1394
AfiI	CCNNNNNNNGG	3 cut(s)	92, 1014, 1538
AgsI	TTSAA	7 cut(s)	29, 117, 196, 392, 445, 696, 1233
AjnI	CCWGG	2 cut(s)	1417, 1467
AleI	CACNNNNGTG	1 cut(s)	53
AloI	GAACNNNNNNTCC	2 cut(s)	676, 708
Alw21I	GWGCWC	3 cut(s)	69, 252, 1134
Alw26I	GTCTC	3 cut(s)	333, 422, 924
AlwI	GGATC	1 cut(s)	531
AlwNI	CAGNNNCTG	2 cut(s)	707, 902
Ama87I	CYCGRG	1 cut(s)	1155
AoxI	GGCC	7 cut(s)	477, 956, 1280, 1415, 1435, 1448, 1533
ApeKI	GCWGC	6 cut(s)	107, 137, 362, 668, 1020, 1339
ApoI	RAATTY	2 cut(s)	303, 859
ArsI	GACNNNNNNTTYG	2 cut(s)	1094, 1126
AspLEI	GCGC	2 cut(s)	142, 1124
AspS9I	GGNCC	2 cut(s)	956, 1415
AsuC2I	CCSGG	5 cut(s)	584, 955, 1225, 1439, 1452
AsuHPI	GGTGA	5 cut(s)	326, 614, 863, 1025, 1174
AvaI	CYCGRG	1 cut(s)	1155
BanI	GGYRCC	2 cut(s)	1094, 1121
BanII	GRGCYC	2 cut(s)	69, 252
Bbv12I	GWGCWC	3 cut(s)	69, 252, 1134
BbvI	GCAGC	6 cut(s)	94, 124, 349, 680, 1032, 1351
BccI	CCATC	5 cut(s)	668, 818, 867, 1193, 1508
BceAI	ACGGC	3 cut(s)	158, 1206, 1411
BcgI	CGANNNNNNTGC	2 cut(s)	986, 1020
BciT130I	CCWGG	2 cut(s)	1419, 1469
BciVI	GTATCC	1 cut(s)	1448
BclI	TGATCA	5 cut(s)	88, 183, 571, 604, 634
BcnI	CCSGG	5 cut(s)	584, 955, 1225, 1439, 1452
BcoDI	GTCTC	3 cut(s)	333, 422, 924
BfaI	CTAG	5 cut(s)	624, 1299, 1334, 1344, 1485
BfmI	CTRYAG	2 cut(s)	727, 840
BfoI	RGCGCY	1 cut(s)	1125
BfuI	GTATCC	1 cut(s)	1448
BglI	GCCNNNNNGGC	1 cut(s)	1481
BisI	GCNGC	7 cut(s)	108, 138, 363, 669, 1021, 1125, 1340
BlpI	GCTNAGC	1 cut(s)	63
BlsI	GCNGC	7 cut(s)	109, 139, 364, 670, 1022, 1126, 1341
Bme1390I	CCNGG	7 cut(s)	584, 955, 1225, 1419, 1439, 1452, 1469
BmeT110I	CYCGRG	1 cut(s)	1155
BmgT120I	GGNCC	2 cut(s)	956, 1415
BmiI	GGNNCC	3 cut(s)	84, 1096, 1123
BmrFI	CCNGG	7 cut(s)	584, 955, 1225, 1419, 1439, 1452, 1469
BmrI	ACTGGG	1 cut(s)	690
BmsI	GCATC	3 cut(s)	1222, 1433, 1462
BmuI	ACTGGG	1 cut(s)	690
Bpu10I	CCTNAGC	2 cut(s)	143, 1194
Bpu1102I	GCTNAGC	1 cut(s)	63
BpuEI	CTTGAG	1 cut(s)	1376
BpuMI	CCSGG	5 cut(s)	584, 955, 1225, 1439, 1452
Bsa29I	ATCGAT	1 cut(s)	1512
BsaHI	GRCGYC	2 cut(s)	415, 1122
BsaJI	CCNNGG	3 cut(s)	518, 1125, 1283
BsaWI	WCCGGW	1 cut(s)	1014
Bsc4I	CCNNNNNNNGG	3 cut(s)	92, 1014, 1538
Bse118I	RCCGGY	1 cut(s)	1531
Bse1I	ACTGG	3 cut(s)	685, 853, 1543
Bse3DI	GCAATG	1 cut(s)	1123
BseBI	CCWGG	2 cut(s)	1419, 1469
BseCI	ATCGAT	1 cut(s)	1512
BseDI	CCNNGG	3 cut(s)	518, 1125, 1283
BseGI	GGATG	5 cut(s)	597, 829, 878, 1464, 1477
BseLI	CCNNNNNNNGG	3 cut(s)	92, 1014, 1538
BseMI	GCAATG	1 cut(s)	1123
BseMII	CTCAG	4 cut(s)	54, 261, 347, 1208
BseNI	ACTGG	3 cut(s)	685, 853, 1543
BseX3I	CGGCCG	1 cut(s)	1435
BseXI	GCAGC	6 cut(s)	94, 124, 349, 680, 1032, 1351
BsgI	GTGCAG	2 cut(s)	1039, 1358
Bsh1236I	CGCG	1 cut(s)	1127
Bsh1285I	CGRYCG	1 cut(s)	1438
BshFI	GGCC	7 cut(s)	479, 958, 1282, 1417, 1437, 1450, 1535
BshNI	GGYRCC	2 cut(s)	1094, 1121
BshVI	ATCGAT	1 cut(s)	1512
BsiEI	CGRYCG	1 cut(s)	1438
BsiHKAI	GWGCWC	3 cut(s)	69, 252, 1134
BsiHKCI	CYCGRG	1 cut(s)	1155
BsiSI	CCGG	8 cut(s)	584, 954, 1015, 1224, 1434, 1438, 1451, 1532
BslFI	GGGAC	2 cut(s)	691, 1145
BslI	CCNNNNNNNGG	3 cut(s)	92, 1014, 1538
BsmAI	GTCTC	3 cut(s)	333, 422, 924
BsmBI	CGTCTC	1 cut(s)	422
BsmFI	GGGAC	2 cut(s)	691, 1145
BsnI	GGCC	7 cut(s)	479, 958, 1282, 1417, 1437, 1450, 1535
BsoBI	CYCGRG	1 cut(s)	1155
Bsp1286I	GDGCHC	3 cut(s)	69, 252, 1134
Bsp1407I	TGTACA	1 cut(s)	483
Bsp143I	GATC	6 cut(s)	88, 183, 523, 571, 604, 634
Bsp1720I	GCTNAGC	1 cut(s)	63
Bsp19I	CCATGG	1 cut(s)	1283
BspACI	CCGC	3 cut(s)	1125, 1127, 1211
BspANI	GGCC	7 cut(s)	479, 958, 1282, 1417, 1437, 1450, 1535
BspCNI	CTCAG	4 cut(s)	55, 262, 348, 1207
BspDI	ATCGAT	1 cut(s)	1512
BspFNI	CGCG	1 cut(s)	1127
BspLI	GGNNCC	3 cut(s)	84, 1096, 1123
BspPI	GGATC	1 cut(s)	531
BspT107I	GGYRCC	2 cut(s)	1094, 1121
BsrDI	GCAATG	1 cut(s)	1123
BsrFI	RCCGGY	1 cut(s)	1531
BsrGI	TGTACA	1 cut(s)	483
BsrI	ACTGG	3 cut(s)	685, 853, 1543
BssAI	RCCGGY	1 cut(s)	1531
BssECI	CCNNGG	3 cut(s)	518, 1125, 1283
BssMI	GATC	6 cut(s)	88, 183, 523, 571, 604, 634
BssNI	GRCGYC	2 cut(s)	415, 1122
BssT1I	CCWWGG	2 cut(s)	518, 1283
Bst2UI	CCWGG	2 cut(s)	1419, 1469
Bst4CI	ACNGT	7 cut(s)	692, 898, 1076, 1306, 1367, 1498, 1555
Bst6I	CTCTTC	1 cut(s)	1065
BstACI	GRCGYC	2 cut(s)	415, 1122
BstAPI	GCANNNNNTGC	2 cut(s)	170, 899
BstAUI	TGTACA	1 cut(s)	483
BstC8I	GCNNGC	2 cut(s)	60, 1533
BstDEI	CTNAG	8 cut(s)	63, 131, 143, 270, 356, 368, 983, 1194
BstDSI	CCRYGG	2 cut(s)	1125, 1283
BstEII	GGTNACC	3 cut(s)	851, 1031, 1180
BstENI	CCTNNNNNAGG	1 cut(s)	90
BstF5I	GGATG	5 cut(s)	597, 829, 878, 1464, 1477
BstFNI	CGCG	1 cut(s)	1127
BstH2I	RGCGCY	1 cut(s)	1125
BstHHI	GCGC	2 cut(s)	142, 1124
BstKTI	GATC	6 cut(s)	91, 186, 526, 574, 607, 637
BstMAI	GTCTC	3 cut(s)	333, 422, 924
BstMBI	GATC	6 cut(s)	88, 183, 523, 571, 604, 634
BstMCI	CGRYCG	1 cut(s)	1438
BstMWI	GCNNNNNNNGC	5 cut(s)	64, 170, 899, 1288, 1481
BstNI	CCWGG	2 cut(s)	1419, 1469
BstNSI	RCATGY	2 cut(s)	387, 653
BstPI	GGTNACC	3 cut(s)	851, 1031, 1180
BstSCI	CCNGG	7 cut(s)	582, 953, 1223, 1417, 1437, 1450, 1467
BstSFI	CTRYAG	2 cut(s)	727, 840
BstUI	CGCG	1 cut(s)	1127
BstV1I	GCAGC	6 cut(s)	94, 124, 349, 680, 1032, 1351
BstZI	CGGCCG	1 cut(s)	1435
Bsu15I	ATCGAT	1 cut(s)	1512
BsuI	GTATCC	1 cut(s)	1448
BsuRI	GGCC	7 cut(s)	479, 958, 1282, 1417, 1437, 1450, 1535
BsuTUI	ATCGAT	1 cut(s)	1512
BtgI	CCRYGG	2 cut(s)	1125, 1283
BtgZI	GCGATG	1 cut(s)	549
BtsCI	GGATG	5 cut(s)	597, 829, 878, 1464, 1477
BtsIMutI	CAGTG	3 cut(s)	152, 1494, 1536
Cac8I	GCNNGC	2 cut(s)	60, 1533
CaiI	CAGNNNCTG	2 cut(s)	707, 902
CfoI	GCGC	2 cut(s)	142, 1124
Cfr10I	RCCGGY	1 cut(s)	1531
Cfr13I	GGNCC	2 cut(s)	956, 1415
Cfr42I	CCGCGG	1 cut(s)	1128
ClaI	ATCGAT	1 cut(s)	1512
CseI	GACGC	1 cut(s)	113
Csp6I	GTAC	5 cut(s)	484, 688, 742, 1363, 1393
CviQI	GTAC	5 cut(s)	484, 688, 742, 1363, 1393
DdeI	CTNAG	8 cut(s)	63, 131, 143, 270, 356, 368, 983, 1194
DinI	GGCGCC	1 cut(s)	1123
DpnI	GATC	6 cut(s)	90, 185, 525, 573, 606, 636
DpnII	GATC	6 cut(s)	88, 183, 523, 571, 604, 634
DraI	TTTAAA	1 cut(s)	511
EaeI	YGGCCR	3 cut(s)	1435, 1448, 1533
EagI	CGGCCG	1 cut(s)	1435
Eam1104I	CTCTTC	1 cut(s)	1065
EarI	CTCTTC	1 cut(s)	1065
Ecl136II	GAGCTC	2 cut(s)	67, 250
EclXI	CGGCCG	1 cut(s)	1435
Eco130I	CCWWGG	2 cut(s)	518, 1283
Eco24I	GRGCYC	2 cut(s)	69, 252
Eco52I	CGGCCG	1 cut(s)	1435
Eco53kI	GAGCTC	2 cut(s)	67, 250
Eco88I	CYCGRG	1 cut(s)	1155
Eco91I	GGTNACC	3 cut(s)	851, 1031, 1180
EcoICRI	GAGCTC	2 cut(s)	67, 250
EcoNI	CCTNNNNNAGG	1 cut(s)	90
EcoO65I	GGTNACC	3 cut(s)	851, 1031, 1180
EcoRI	GAATTC	1 cut(s)	303
EcoRII	CCWGG	2 cut(s)	1417, 1467
EcoT14I	CCWWGG	2 cut(s)	518, 1283
EcoT22I	ATGCAT	1 cut(s)	651
EcoT38I	GRGCYC	2 cut(s)	69, 252
EgeI	GGCGCC	1 cut(s)	1123
EheI	GGCGCC	1 cut(s)	1123
ErhI	CCWWGG	2 cut(s)	518, 1283
Esp3I	CGTCTC	1 cut(s)	422
FalI	AAGNNNNNCTT	2 cut(s)	425, 457
FaqI	GGGAC	2 cut(s)	691, 1145
FbaI	TGATCA	5 cut(s)	88, 183, 571, 604, 634
Fnu4HI	GCNGC	7 cut(s)	108, 138, 363, 669, 1021, 1125, 1340
FokI	GGATG	5 cut(s)	604, 836, 885, 1451, 1484
FriOI	GRGCYC	2 cut(s)	69, 252
Fsp4HI	GCNGC	7 cut(s)	108, 138, 363, 669, 1021, 1125, 1340
FspBI	CTAG	5 cut(s)	624, 1299, 1334, 1344, 1485
GlaI	GCGC	2 cut(s)	141, 1123
GluI	GCNGC	7 cut(s)	108, 138, 363, 669, 1021, 1125, 1340
HaeII	RGCGCY	1 cut(s)	1125
HaeIII	GGCC	7 cut(s)	479, 958, 1282, 1417, 1437, 1450, 1535
HapII	CCGG	8 cut(s)	584, 954, 1015, 1224, 1434, 1438, 1451, 1532
HgaI	GACGC	1 cut(s)	113
HhaI	GCGC	2 cut(s)	142, 1124
Hin1I	GRCGYC	2 cut(s)	415, 1122
Hin6I	GCGC	2 cut(s)	140, 1122
HinP1I	GCGC	2 cut(s)	140, 1122
HincII	GTYRAC	2 cut(s)	178, 991
HindII	GTYRAC	2 cut(s)	178, 991
HindIII	AAGCTT	3 cut(s)	348, 439, 1289
HinfI	GANTC	4 cut(s)	157, 656, 992, 1158
HpaII	CCGG	8 cut(s)	584, 954, 1015, 1224, 1434, 1438, 1451, 1532
HphI	GGTGA	5 cut(s)	326, 614, 863, 1025, 1174
Hpy166II	GTNNAC	6 cut(s)	178, 328, 486, 629, 991, 1551
Hpy188I	TCNGA	5 cut(s)	271, 309, 528, 997, 1203
Hpy188III	TCNNGA	5 cut(s)	101, 117, 419, 608, 909
Hpy8I	GTNNAC	6 cut(s)	178, 328, 486, 629, 991, 1551
HpyAV	CCTTC	4 cut(s)	329, 386, 754, 1018
HpyCH4III	ACNGT	7 cut(s)	692, 898, 1076, 1306, 1367, 1498, 1555
HpyCH4IV	ACGT	1 cut(s)	415
HpyF10VI	GCNNNNNNNGC	5 cut(s)	64, 170, 899, 1288, 1481
HpyF3I	CTNAG	8 cut(s)	63, 131, 143, 270, 356, 368, 983, 1194
HpySE526I	ACGT	1 cut(s)	415
Hsp92I	GRCGYC	2 cut(s)	415, 1122
HspAI	GCGC	2 cut(s)	140, 1122
KasI	GGCGCC	1 cut(s)	1121
KroI	GCCGGC	1 cut(s)	1531
KroNI	GCCGGC	1 cut(s)	1533
Ksp22I	TGATCA	5 cut(s)	88, 183, 571, 604, 634
KspI	CCGCGG	1 cut(s)	1128
Kzo9I	GATC	6 cut(s)	88, 183, 523, 571, 604, 634
LmnI	GCTCC	6 cut(s)	72, 82, 247, 255, 724, 1137
Lsp1109I	GCAGC	6 cut(s)	94, 124, 349, 680, 1032, 1351
LweI	GCATC	3 cut(s)	1222, 1433, 1462
MaeI	CTAG	5 cut(s)	624, 1299, 1334, 1344, 1485
MaeII	ACGT	1 cut(s)	415
MalI	GATC	6 cut(s)	90, 185, 525, 573, 606, 636
MboI	GATC	6 cut(s)	88, 183, 523, 571, 604, 634
MboII	GAAGA	8 cut(s)	105, 769, 802, 805, 808, 1003, 1052, 1483
MfeI	CAATTG	1 cut(s)	546
MhlI	GDGCHC	3 cut(s)	69, 252, 1134
MluCI	AATT	7 cut(s)	24, 303, 491, 546, 577, 859, 1067
Mly113I	GGCGCC	1 cut(s)	1122
MlyI	GAGTC	2 cut(s)	986, 1167
MmeI	TCCRAC	2 cut(s)	1020, 1181
Mph1103I	ATGCAT	1 cut(s)	651
MroNI	GCCGGC	1 cut(s)	1531
MseI	TTAA	5 cut(s)	344, 510, 618, 711, 1545
MslI	CAYNNNNRTG	3 cut(s)	53, 785, 1322
MspA1I	CMGCKG	1 cut(s)	1127
MspI	CCGG	8 cut(s)	584, 954, 1015, 1224, 1434, 1438, 1451, 1532
MspR9I	CCNGG	7 cut(s)	584, 955, 1225, 1419, 1439, 1452, 1469
MunI	CAATTG	1 cut(s)	546
MvaI	CCWGG	2 cut(s)	1419, 1469
MvnI	CGCG	1 cut(s)	1127
MwoI	GCNNNNNNNGC	5 cut(s)	64, 170, 899, 1288, 1481
NaeI	GCCGGC	1 cut(s)	1533
NarI	GGCGCC	1 cut(s)	1122
NciI	CCSGG	5 cut(s)	584, 955, 1225, 1439, 1452
NcoI	CCATGG	1 cut(s)	1283
NdeII	GATC	6 cut(s)	88, 183, 523, 571, 604, 634
NgoMIV	GCCGGC	1 cut(s)	1531
NlaIV	GGNNCC	3 cut(s)	84, 1096, 1123
NmuCI	GTSAC	6 cut(s)	314, 451, 851, 1031, 1082, 1180
NsiI	ATGCAT	1 cut(s)	651
NspI	RCATGY	2 cut(s)	387, 653
OliI	CACNNNNGTG	1 cut(s)	53
PaeR7I	CTCGAG	1 cut(s)	1155
PdiI	GCCGGC	1 cut(s)	1533
PfeI	GAWTC	2 cut(s)	157, 656
PfoI	TCCNGGA	1 cut(s)	1467
PkrI	GCNGC	7 cut(s)	109, 139, 364, 670, 1022, 1126, 1341
PleI	GAGTC	2 cut(s)	986, 1166
PluTI	GGCGCC	1 cut(s)	1125
PpsI	GAGTC	2 cut(s)	986, 1166
Psp124BI	GAGCTC	2 cut(s)	69, 252
Psp6I	CCWGG	2 cut(s)	1417, 1467
PspEI	GGTNACC	3 cut(s)	851, 1031, 1180
PspGI	CCWGG	2 cut(s)	1417, 1467
PspN4I	GGNNCC	3 cut(s)	84, 1096, 1123
PspPI	GGNCC	2 cut(s)	956, 1415
PspXI	VCTCGAGB	1 cut(s)	1155
PstNI	CAGNNNCTG	2 cut(s)	707, 902
RsaI	GTAC	5 cut(s)	485, 689, 743, 1364, 1394
RsaNI	GTAC	5 cut(s)	484, 688, 742, 1363, 1393
RseI	CAYNNNNRTG	3 cut(s)	53, 785, 1322
SacI	GAGCTC	2 cut(s)	69, 252
SacII	CCGCGG	1 cut(s)	1128
SaqAI	TTAA	5 cut(s)	344, 510, 618, 711, 1545
SatI	GCNGC	7 cut(s)	108, 138, 363, 669, 1021, 1125, 1340
Sau3AI	GATC	6 cut(s)	88, 183, 523, 571, 604, 634
Sau96I	GGNCC	2 cut(s)	956, 1415
SchI	GAGTC	2 cut(s)	986, 1167
ScrFI	CCNGG	7 cut(s)	584, 955, 1225, 1419, 1439, 1452, 1469
SduI	GDGCHC	3 cut(s)	69, 252, 1134
SfaNI	GCATC	3 cut(s)	1222, 1433, 1462
SfcI	CTRYAG	2 cut(s)	727, 840
SfoI	GGCGCC	1 cut(s)	1123
Sfr274I	CTCGAG	1 cut(s)	1155
Sfr303I	CCGCGG	1 cut(s)	1128
SgrBI	CCGCGG	1 cut(s)	1128
SlaI	CTCGAG	1 cut(s)	1155
SmiMI	CAYNNNNRTG	3 cut(s)	53, 785, 1322
SmlI	CTYRAG	2 cut(s)	1155, 1355
SmoI	CTYRAG	2 cut(s)	1155, 1355
Sse9I	AATT	7 cut(s)	24, 303, 491, 546, 577, 859, 1067
SsiI	CCGC	3 cut(s)	1125, 1127, 1211
SspDI	GGCGCC	1 cut(s)	1121
SspMI	CTAG	5 cut(s)	624, 1299, 1334, 1344, 1485
SstI	GAGCTC	2 cut(s)	69, 252
StyD4I	CCNGG	7 cut(s)	582, 953, 1223, 1417, 1437, 1450, 1467
StyI	CCWWGG	2 cut(s)	518, 1283
TaaI	ACNGT	7 cut(s)	692, 898, 1076, 1306, 1367, 1498, 1555
TaiI	ACGT	1 cut(s)	418
TaqI	TCGA	3 cut(s)	420, 1156, 1512
TaqII	GACCGA	1 cut(s)	870
TasI	AATT	7 cut(s)	24, 303, 491, 546, 577, 859, 1067
TatI	WGTACW	1 cut(s)	483
TauI	GCSGC	1 cut(s)	1127
TfiI	GAWTC	2 cut(s)	157, 656
Tru1I	TTAA	5 cut(s)	344, 510, 618, 711, 1545
Tru9I	TTAA	5 cut(s)	344, 510, 618, 711, 1545
TscAI	CASTG	3 cut(s)	159, 1501, 1543
TseFI	GTSAC	6 cut(s)	314, 451, 851, 1031, 1082, 1180
TseI	GCWGC	6 cut(s)	107, 137, 362, 668, 1020, 1339
Tsp45I	GTSAC	6 cut(s)	314, 451, 851, 1031, 1082, 1180
TspRI	CASTG	3 cut(s)	159, 1501, 1543
XagI	CCTNNNNNAGG	1 cut(s)	90
XapI	RAATTY	2 cut(s)	303, 859
XceI	RCATGY	2 cut(s)	387, 653
XhoI	CTCGAG	1 cut(s)	1155
XspI	CTAG	5 cut(s)	624, 1299, 1334, 1344, 1485
ZraI	GACGTC	1 cut(s)	416
Zsp2I	ATGCAT	1 cut(s)	651

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.