Rroxscaffold_6G00422790

Acts in the modification of cell walls via demethylesterification of cell wall pectin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014881
Physical Location & Seq
Forward (+)
43605307 .. 43607506
2200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422790.1

Sequence Viewer

Length: 1041 bp
ATGAATACTTTGACAATTGCGAATAAGAGTTCATCGCCGGATCATAATTTTCCGGCATGGATGAGTGAGGGTGATCAAGAGCTGGTTAAGTCAAGTCCACATGATCAGTCAGGTATGCATGTAGATGCTGTTGTGGCAACAGATGGGAGCGGGAAGTACCGTTCAATCACAGAAGCTGTTAATGAAGCTCCAGACCACGGCAGGAGGAGGTACATAATATATGTGAAGAAGGGGATTTATAGAGAAAACATTGACATGAAGAAGAAGAAAACCAATATCATGTTTGTAGGGGATGGAATTGGACAAACTGTGGTAACTGGTGACCGAAGTTTCATGCAAGGATGGACTACATTTAGAACTGCAACAGTTGCTGTATCCGGAAAAGGATTTATAGCAAGAGACATGACTTTTCGTAACACGGCCGGGCCTGAAAACCATCAAGGCGTGGCGCTTAGGGTTGACTCGGACCAATCTGCCTTCTTCCGGTGCAGCATGGAGGGTCACCAAGACACACTGTATGCTCACTCTCTTCGCCAATTTTATCGTGAATGTGACATCTATGGTACCATAGACTTCATATTTGGCAATGGTGCCGCGGTGCTCCAAAATTGCAAAATCTACACTCGAGTCCCGCTATCACTACAAAAGGTCACCATCACGGCCCAGGGTCGGAAAGATCCGCATCAAAACACTGGATTTTCGATCCAAGATAGCTATGTTTTTGCTACTCAACCGACATATCTGGGGAGGCCATGGAAGCCATACTCTAGGACAGTTTTTATCAACACTTACTTGAGTGGTCTAGTGCAGCCTAGAGGGTGGCTTGAGTGGTATGGTAACTTTGCTTTGGGCACATTATGGTACGGCGAGTATAGAAACTATGGGCCGGGTGCATCACTATCCGGACGGGTCAAGTGGCCAGGTTACCACATCATTCAGGATGCCAACCTGGCTAGTTTCTTCACGGTTCGGCGATTTATTGATGGGATGGCTTGGTTGCCGGCCACAGGTGTTAAGTTTACAGTAGGTTTGAGAAACTAA

Protein Analysis

346

Amino Acids

38.93

Weight (kDa)

9.41

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 42 - 329 3.7e-139 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05620
fragaria_vesca FvH4_6g06871
malus_domestica MD04G1198000.v1.1 MD12G1210600.v1.1
prunus_persica Prupe.6G318500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0456351
rosa_laevigata RLG00000025310
rosa_multiflora Rmu_sc0002607.1_g000020 Rmu_sc0018054.1_g000002
rosa_roxburghii Rroxscaffold_6G00422790
rosa_rugosa Rorug03G0012700
rosa_samantha Rh3AG072800 Rh3BG074900 Rh3CG074500 Rh3DG076300
rosa_wichuraiana Rw3G006250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 563
AccB1I GGYRCC 2 cut(s) 563, 590
AccBSI CCGCTC 1 cut(s) 150
AccII CGCG 1 cut(s) 596
AccIII TCCGGA 2 cut(s) 377, 902
AciI CCGC 5 cut(s) 150, 594, 596, 632, 680
AclWI GGATC 3 cut(s) 48, 671, 697
AcoI YGGCCR 3 cut(s) 420, 917, 1002
AfaI GTAC 4 cut(s) 158, 212, 565, 863
AfiI CCNNNNNNNGG 4 cut(s) 197, 483, 669, 1007
AgsI TTSAA 1 cut(s) 165
AjnI CCWGG 3 cut(s) 663, 919, 948
AloI GAACNNNNNNTCC 2 cut(s) 145, 177
AluBI AGCT 4 cut(s) 82, 176, 188, 714
AluI AGCT 4 cut(s) 82, 176, 188, 714
Alw21I GWGCWC 1 cut(s) 603
Alw26I GTCTC 1 cut(s) 393
AlwI GGATC 3 cut(s) 48, 671, 697
AlwNI CAGNNNCTG 2 cut(s) 176, 371
Ama87I CYCGRG 1 cut(s) 624
Aor13HI TCCGGA 2 cut(s) 377, 902
AoxI GGCC 7 cut(s) 420, 425, 660, 749, 884, 917, 1002
ApeKI GCWGC 2 cut(s) 489, 808
ArsI GACNNNNNNTTYG 2 cut(s) 563, 595
Asp718I GGTACC 1 cut(s) 563
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 4 cut(s) 425, 466, 661, 884
AsuC2I CCSGG 2 cut(s) 424, 888
AsuHPI GGTGA 4 cut(s) 83, 332, 494, 643
AvaI CYCGRG 1 cut(s) 624
AvaII GGWCC 1 cut(s) 466
BaeGI GKGCMC 1 cut(s) 854
BalI TGGCCA 1 cut(s) 919
BanI GGYRCC 2 cut(s) 563, 590
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 2 cut(s) 501, 820
BccI CCATC 7 cut(s) 137, 287, 336, 444, 662, 977, 982
BceAI ACGGC 4 cut(s) 214, 435, 675, 880
BciT130I CCWGG 3 cut(s) 665, 921, 950
BciVI GTATCC 1 cut(s) 385
BclI TGATCA 2 cut(s) 73, 103
BcnI CCSGG 2 cut(s) 424, 888
BcoDI GTCTC 1 cut(s) 393
BfaI CTAG 4 cut(s) 768, 803, 813, 954
BfoI RGCGCY 1 cut(s) 452
BfuI GTATCC 1 cut(s) 385
BglI GCCNNNNNGGC 1 cut(s) 950
BisI GCNGC 3 cut(s) 490, 594, 809
BlsI GCNGC 3 cut(s) 491, 595, 810
Bme1390I CCNGG 5 cut(s) 424, 665, 888, 921, 950
Bme18I GGWCC 1 cut(s) 466
BmeT110I CYCGRG 1 cut(s) 624
BmgT120I GGNCC 4 cut(s) 425, 466, 661, 884
BmiI GGNNCC 2 cut(s) 565, 592
BmrFI CCNGG 5 cut(s) 424, 665, 888, 921, 950
BmsI GCATC 4 cut(s) 115, 691, 902, 931
BpmI CTGGAG 1 cut(s) 174
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 2 cut(s) 814, 845
BpuMI CCSGG 2 cut(s) 424, 888
BsaBI GATNNNNATC 1 cut(s) 681
BsaJI CCNNGG 5 cut(s) 196, 594, 663, 664, 752
BsaWI WCCGGW 3 cut(s) 377, 483, 902
Bsc4I CCNNNNNNNGG 4 cut(s) 197, 483, 669, 1007
Bse118I RCCGGY 1 cut(s) 1000
Bse1I ACTGG 2 cut(s) 322, 697
Bse3DI GCAATG 1 cut(s) 592
Bse8I GATNNNNATC 1 cut(s) 681
BseAI TCCGGA 2 cut(s) 377, 902
BseBI CCWGG 3 cut(s) 665, 921, 950
BseDI CCNNGG 5 cut(s) 196, 594, 663, 664, 752
BseGI GGATG 5 cut(s) 66, 298, 347, 946, 993
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 4 cut(s) 197, 483, 669, 1007
BseMI GCAATG 1 cut(s) 592
BseNI ACTGG 2 cut(s) 322, 697
BseRI GAGGAG 1 cut(s) 220
BseSI GKGCMC 1 cut(s) 854
BseX3I CGGCCG 1 cut(s) 420
BseXI GCAGC 2 cut(s) 501, 820
BsgI GTGCAG 2 cut(s) 508, 827
Bsh1236I CGCG 1 cut(s) 596
Bsh1285I CGRYCG 1 cut(s) 423
BshFI GGCC 7 cut(s) 422, 427, 662, 751, 886, 919, 1004
BshNI GGYRCC 2 cut(s) 563, 590
BsiEI CGRYCG 1 cut(s) 423
BsiHKAI GWGCWC 1 cut(s) 603
BsiHKCI CYCGRG 1 cut(s) 624
BsiSI CCGG 8 cut(s) 38, 53, 378, 423, 484, 887, 903, 1001
BslFI GGGAC 1 cut(s) 614
BslI CCNNNNNNNGG 4 cut(s) 197, 483, 669, 1007
BsmAI GTCTC 1 cut(s) 393
BsmFI GGGAC 1 cut(s) 614
BsnI GGCC 7 cut(s) 422, 427, 662, 751, 886, 919, 1004
BsoBI CYCGRG 1 cut(s) 624
Bsp1286I GDGCHC 2 cut(s) 603, 854
Bsp13I TCCGGA 2 cut(s) 377, 902
Bsp143I GATC 5 cut(s) 40, 73, 103, 676, 702
Bsp19I CCATGG 1 cut(s) 752
BspACI CCGC 5 cut(s) 150, 594, 596, 632, 680
BspANI GGCC 7 cut(s) 422, 427, 662, 751, 886, 919, 1004
BspEI TCCGGA 2 cut(s) 377, 902
BspFNI CGCG 1 cut(s) 596
BspLI GGNNCC 2 cut(s) 565, 592
BspPI GGATC 3 cut(s) 48, 671, 697
BspT107I GGYRCC 2 cut(s) 563, 590
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 592
BsrFI RCCGGY 1 cut(s) 1000
BsrI ACTGG 2 cut(s) 322, 697
BssAI RCCGGY 1 cut(s) 1000
BssECI CCNNGG 5 cut(s) 196, 594, 663, 664, 752
BssMI GATC 5 cut(s) 40, 73, 103, 676, 702
BssT1I CCWWGG 1 cut(s) 752
Bst2UI CCWGG 3 cut(s) 665, 921, 950
Bst4CI ACNGT 7 cut(s) 161, 310, 367, 516, 775, 967, 1024
Bst6I CTCTTC 1 cut(s) 534
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 1002
BstDEI CTNAG 1 cut(s) 452
BstDSI CCRYGG 3 cut(s) 196, 594, 752
BstEII GGTNACC 4 cut(s) 320, 500, 649, 923
BstF5I GGATG 5 cut(s) 66, 298, 347, 946, 993
BstFNI CGCG 1 cut(s) 596
BstH2I RGCGCY 1 cut(s) 452
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 5 cut(s) 43, 76, 106, 679, 705
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 5 cut(s) 40, 73, 103, 676, 702
BstMCI CGRYCG 1 cut(s) 423
BstMWI GCNNNNNNNGC 4 cut(s) 134, 368, 757, 950
BstNI CCWGG 3 cut(s) 665, 921, 950
BstNSI RCATGY 1 cut(s) 122
BstPI GGTNACC 4 cut(s) 320, 500, 649, 923
BstSCI CCNGG 5 cut(s) 422, 663, 886, 919, 948
BstSLI GKGCMC 1 cut(s) 854
BstUI CGCG 1 cut(s) 596
BstV1I GCAGC 2 cut(s) 501, 820
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BstZI CGGCCG 1 cut(s) 420
BsuI GTATCC 1 cut(s) 385
BsuRI GGCC 7 cut(s) 422, 427, 662, 751, 886, 919, 1004
BtgI CCRYGG 3 cut(s) 196, 594, 752
BtgZI GCGATG 1 cut(s) 18
BtsCI GGATG 5 cut(s) 66, 298, 347, 946, 993
BtsIMutI CAGTG 2 cut(s) 512, 690
Cac8I GCNNGC 1 cut(s) 1002
CaiI CAGNNNCTG 2 cut(s) 176, 371
CfoI GCGC 1 cut(s) 451
Cfr10I RCCGGY 1 cut(s) 1000
Cfr13I GGNCC 4 cut(s) 425, 466, 661, 884
Cfr42I CCGCGG 1 cut(s) 597
Csp6I GTAC 4 cut(s) 157, 211, 564, 862
CviAII CATG 9 cut(s) 57, 101, 119, 256, 280, 334, 403, 493, 753
CviQI GTAC 4 cut(s) 157, 211, 564, 862
DdeI CTNAG 1 cut(s) 452
DpnI GATC 5 cut(s) 42, 75, 105, 678, 704
DpnII GATC 5 cut(s) 40, 73, 103, 676, 702
EaeI YGGCCR 3 cut(s) 420, 917, 1002
EagI CGGCCG 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
EclXI CGGCCG 1 cut(s) 420
Eco130I CCWWGG 1 cut(s) 752
Eco47I GGWCC 1 cut(s) 466
Eco52I CGGCCG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 624
Eco91I GGTNACC 4 cut(s) 320, 500, 649, 923
EcoO65I GGTNACC 4 cut(s) 320, 500, 649, 923
EcoRII CCWGG 3 cut(s) 663, 919, 948
EcoT14I CCWWGG 1 cut(s) 752
EcoT22I ATGCAT 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 752
FaeI CATG 9 cut(s) 60, 104, 122, 259, 283, 337, 406, 496, 756
FaqI GGGAC 1 cut(s) 614
FatI CATG 9 cut(s) 56, 100, 118, 255, 279, 333, 402, 492, 752
FauI CCCGC 2 cut(s) 143, 639
FbaI TGATCA 2 cut(s) 73, 103
Fnu4HI GCNGC 3 cut(s) 490, 594, 809
FokI GGATG 5 cut(s) 73, 305, 354, 953, 1000
Fsp4HI GCNGC 3 cut(s) 490, 594, 809
FspBI CTAG 4 cut(s) 768, 803, 813, 954
GlaI GCGC 1 cut(s) 450
GluI GCNGC 3 cut(s) 490, 594, 809
GsuI CTGGAG 1 cut(s) 174
HaeII RGCGCY 1 cut(s) 452
HaeIII GGCC 7 cut(s) 422, 427, 662, 751, 886, 919, 1004
HapII CCGG 8 cut(s) 38, 53, 378, 423, 484, 887, 903, 1001
HhaI GCGC 1 cut(s) 451
Hin1II CATG 9 cut(s) 60, 104, 122, 259, 283, 337, 406, 496, 756
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 2 cut(s) 461, 627
HpaII CCGG 8 cut(s) 38, 53, 378, 423, 484, 887, 903, 1001
HphI GGTGA 4 cut(s) 83, 332, 494, 643
Hpy166II GTNNAC 3 cut(s) 98, 460, 1020
Hpy188I TCNGA 2 cut(s) 466, 672
Hpy188III TCNNGA 6 cut(s) 77, 191, 378, 545, 903, 938
Hpy8I GTNNAC 3 cut(s) 98, 460, 1020
HpyAV CCTTC 2 cut(s) 223, 487
HpyCH4III ACNGT 7 cut(s) 161, 310, 367, 516, 775, 967, 1024
HpyCH4V TGCA 7 cut(s) 118, 337, 362, 489, 612, 808, 893
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 368, 757, 950
HpyF3I CTNAG 1 cut(s) 452
Hsp92II CATG 9 cut(s) 60, 104, 122, 259, 283, 337, 406, 496, 756
HspAI GCGC 1 cut(s) 449
Kpn2I TCCGGA 2 cut(s) 377, 902
KpnI GGTACC 1 cut(s) 567
KroI GCCGGC 1 cut(s) 1000
KroNI GCCGGC 1 cut(s) 1002
Ksp22I TGATCA 2 cut(s) 73, 103
KspI CCGCGG 1 cut(s) 597
Kzo9I GATC 5 cut(s) 40, 73, 103, 676, 702
LmnI GCTCC 3 cut(s) 147, 193, 606
Lsp1109I GCAGC 2 cut(s) 501, 820
LweI GCATC 4 cut(s) 115, 691, 902, 931
MaeI CTAG 4 cut(s) 768, 803, 813, 954
MaeIII GTNAC 8 cut(s) 313, 320, 413, 500, 551, 649, 836, 923
MalI GATC 5 cut(s) 42, 75, 105, 678, 704
MbiI CCGCTC 1 cut(s) 150
MboI GATC 5 cut(s) 40, 73, 103, 676, 702
MboII GAAGA 7 cut(s) 238, 271, 274, 277, 472, 521, 952
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 2 cut(s) 603, 854
MlsI TGGCCA 1 cut(s) 919
MluCI AATT 5 cut(s) 15, 46, 297, 536, 607
MluNI TGGCCA 1 cut(s) 919
MlyI GAGTC 2 cut(s) 455, 636
MmeI TCCRAC 1 cut(s) 650
MnlI CCTC 6 cut(s) 61, 198, 201, 490, 741, 809
Mox20I TGGCCA 1 cut(s) 919
Mph1103I ATGCAT 1 cut(s) 120
MroI TCCGGA 2 cut(s) 377, 902
MroNI GCCGGC 1 cut(s) 1000
MscI TGGCCA 1 cut(s) 919
MseI TTAA 3 cut(s) 87, 180, 1014
MslI CAYNNNNRTG 2 cut(s) 123, 254
Msp20I TGGCCA 1 cut(s) 919
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 8 cut(s) 38, 53, 378, 423, 484, 887, 903, 1001
MspR9I CCNGG 5 cut(s) 424, 665, 888, 921, 950
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 3 cut(s) 665, 921, 950
MvnI CGCG 1 cut(s) 596
MwoI GCNNNNNNNGC 4 cut(s) 134, 368, 757, 950
NaeI GCCGGC 1 cut(s) 1002
NciI CCSGG 2 cut(s) 424, 888
NcoI CCATGG 1 cut(s) 752
NdeII GATC 5 cut(s) 40, 73, 103, 676, 702
NgoMIV GCCGGC 1 cut(s) 1000
NlaIII CATG 9 cut(s) 60, 104, 122, 259, 283, 337, 406, 496, 756
NlaIV GGNNCC 2 cut(s) 565, 592
NmuCI GTSAC 4 cut(s) 320, 500, 551, 649
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 1 cut(s) 122
PaeR7I CTCGAG 1 cut(s) 624
PasI CCCWGGG 1 cut(s) 664
PdiI GCCGGC 1 cut(s) 1002
PkrI GCNGC 3 cut(s) 491, 595, 810
PleI GAGTC 2 cut(s) 455, 635
PpsI GAGTC 2 cut(s) 455, 635
Psp6I CCWGG 3 cut(s) 663, 919, 948
PspEI GGTNACC 4 cut(s) 320, 500, 649, 923
PspGI CCWGG 3 cut(s) 663, 919, 948
PspN4I GGNNCC 2 cut(s) 565, 592
PspPI GGNCC 4 cut(s) 425, 466, 661, 884
PspXI VCTCGAGB 1 cut(s) 624
PstNI CAGNNNCTG 2 cut(s) 176, 371
PsuI RGATCY 1 cut(s) 676
RsaI GTAC 4 cut(s) 158, 212, 565, 863
RsaNI GTAC 4 cut(s) 157, 211, 564, 862
RseI CAYNNNNRTG 2 cut(s) 123, 254
SacII CCGCGG 1 cut(s) 597
SaqAI TTAA 3 cut(s) 87, 180, 1014
SatI GCNGC 3 cut(s) 490, 594, 809
Sau3AI GATC 5 cut(s) 40, 73, 103, 676, 702
Sau96I GGNCC 4 cut(s) 425, 466, 661, 884
SchI GAGTC 2 cut(s) 455, 636
ScrFI CCNGG 5 cut(s) 424, 665, 888, 921, 950
SduI GDGCHC 2 cut(s) 603, 854
SfaNI GCATC 4 cut(s) 115, 691, 902, 931
Sfr274I CTCGAG 1 cut(s) 624
Sfr303I CCGCGG 1 cut(s) 597
SgrBI CCGCGG 1 cut(s) 597
SinI GGWCC 1 cut(s) 466
SlaI CTCGAG 1 cut(s) 624
SmiMI CAYNNNNRTG 2 cut(s) 123, 254
SmlI CTYRAG 3 cut(s) 624, 793, 824
SmoI CTYRAG 3 cut(s) 624, 793, 824
Sse9I AATT 5 cut(s) 15, 46, 297, 536, 607
SsiI CCGC 5 cut(s) 150, 594, 596, 632, 680
SspMI CTAG 4 cut(s) 768, 803, 813, 954
StyD4I CCNGG 5 cut(s) 422, 663, 886, 919, 948
StyI CCWWGG 1 cut(s) 752
TaaI ACNGT 7 cut(s) 161, 310, 367, 516, 775, 967, 1024
TaqI TCGA 2 cut(s) 625, 701
TaqII GACCGA 1 cut(s) 339
TasI AATT 5 cut(s) 15, 46, 297, 536, 607
TauI GCSGC 1 cut(s) 596
Tru1I TTAA 3 cut(s) 87, 180, 1014
Tru9I TTAA 3 cut(s) 87, 180, 1014
TscAI CASTG 2 cut(s) 519, 697
TseFI GTSAC 4 cut(s) 320, 500, 551, 649
TseI GCWGC 2 cut(s) 489, 808
Tsp45I GTSAC 4 cut(s) 320, 500, 551, 649
TspDTI ATGAA 6 cut(s) 17, 21, 198, 272, 322, 565
TspRI CASTG 2 cut(s) 519, 697
VpaK11BI GGWCC 1 cut(s) 466
XceI RCATGY 1 cut(s) 122
XhoI CTCGAG 1 cut(s) 624
XspI CTAG 4 cut(s) 768, 803, 813, 954
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.