Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0014881

Physical Location & Seq

Genomic Coordinates Forward (+)

28574369 .. 28576256

Gene Structure 1888 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1198000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGGCCTTGGCCAATTTTTTGCTTCTCCTACTTTCAATTGCATGTTGTTCTTATGGGGTTACTTCCTCCCATTCCATTCAATCCATGGTTACACAAACTTGCACTGATATCGAAGACCGGAATTCGTGCCTTGCGAATGTGCAAGCCGAGCTCCAAAAAACGGGGCCTCAAATTCAAGATTCGGTGTCGATCATAACTGCCGCTCTTAGGCACACGCTCAATGAAACAAAAAATGCAATCCAAATGATCACAAAGTTCAACTCTTTGTCCATAAGTTACAGGGAGAGTGTAGCAATTGAGGACTGCAAGGAGCTCCTGGACTACTCCGTCTCCGAGTTGGCTTGGTCTCTAGGCGAAATGATCAAAATCCGTGGCGGCGACACAAATGTTCACTACGAAGGAGACTTAAAATCTTGGCTTAGTGCTGCCCTGAGTAACCAAGATACCTGCCTTGAGGGTTTTGAAGGAACCGACCGACGCCTCGAAAGTTTCGTTAGGGGAAGTTTGAAGCAAGTGACGCAGCTCATTGGTAATGTTCTTGCCTTGTACACTCAACTATACAGCTTGCCCTCTCCTCCTAGGGATCATGGTCCCCCGATGAATAAAAGTTCGTCAGGGGATGATTTCCCGGCGTGGATGAGTGAGGATGATCAAGCGCTGGTTAAATCGAATCCAAAATCGGGTGCGAAGCATGCTGTTGTAGCAGCAGATGGGAGTGGACAGTACCGTACGATCACAGAAGCTGTTAACGAAGCTCCAAACTACAGCCCTAGCAGGTACGTTATATATGTGAAGAAGGGAATATATAAGGAAAACATCGATCTGAAGAAGAAGAAAACCAATATTATGTTCGTTGGGGATGGGATCGGACAAACTATCGTCACCGGTAACCGGAATTTCATGCAAGGATGGACGACGTTTAGAACTGCAACAGTTGCGGTATCCGGGAAGGGATTCATAGCAAAAGAGATGACAATTCGCAACACCGCCGGCCCTCAGAACCACCAAGGCGTGGCGCTAAGAGTCGACTCCGACCAGTCGGCCTTCTTCCGGTGCAGCATTGAGGGTAACCAAGACACCCTCTATGCTCACTCCCTCCGGCAGTTCTACCGGGAATGCAGCATCTACGGCACCATAGACTTCATATTTGGCAACGGAGCTGCAGTCCTCCAAAACTGCAAAATCTACACAAGGGTCCCACTCCCGCTACAAAAGGTCACCATCACCGCCCAAGGTAGGAAAAACCCGCATCAAAACACCGGGTTTACGATCCAGGACAGTTTTGTTCTGGCCACCCAGCCAACTTATTTGGGCAGGCCGTGGAAGGAATATTCCAGGACTGTTTATATGAACACTTACATGAGCGGGCTGGTCCAGCCCAGAGGGTGGCTCGAGTGGTATGGGGATTTTGCTTTGAGCACCTTGTGGTATGGTGAGTATAGAAATTATGGGCCGGGTGCGGGTCTAGCGGGTCGGGTCAAATGGCCTGGTTACCATATACTCAAGGATGCCGCCGCCGCGAGCTTCTTCACCGTTGGGAGGTTTATTGACGGGAGGGCTTGGTTGCCGTCGACAGGTGTCAAGTTTACAGCGGGTTTGAGTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

536

Amino Acids

59.22

Weight (kDa)

8.66

Isoelectric Point (pI)

33.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	29 - 182	7.8e-27	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	232 - 518	4.1e-138	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05620
fragaria_vesca	FvH4_6g06871
malus_domestica	MD04G1198000.v1.1 MD12G1210600.v1.1
prunus_persica	Prupe.6G318500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0456351
rosa_laevigata	RLG00000025310
rosa_multiflora	Rmu_sc0002607.1_g000020 Rmu_sc0018054.1_g000002
rosa_roxburghii	Rroxscaffold_6G00422790
rosa_rugosa	Rorug03G0012700
rosa_samantha	Rh3AG072800 Rh3BG074900 Rh3CG074500 Rh3DG076300
rosa_wichuraiana	Rw3G006250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	326
Acc36I	ACCTGC	2 cut(s)	455, 765
AccB1I	GGYRCC	1 cut(s)	1130
AccB7I	CCANNNNNTGG	2 cut(s)	1012, 1386
AccBSI	CCGCTC	2 cut(s)	203, 1365
AccI	GTMKAC	2 cut(s)	1026, 1571
AccII	CGCG	1 cut(s)	1520
AclWI	GGATC	3 cut(s)	591, 872, 1264
AcoI	YGGCCR	2 cut(s)	9, 1290
AcsI	RAATTY	3 cut(s)	121, 171, 895
AcuI	CTGAAG	1 cut(s)	845
AcyI	GRCGYC	1 cut(s)	478
AdeI	CACNNNGTG	1 cut(s)	1425
AfaI	GTAC	4 cut(s)	548, 725, 730, 779
AfeI	AGCGCT	1 cut(s)	657
AgeI	ACCGGT	1 cut(s)	884
AgsI	TTSAA	6 cut(s)	36, 80, 176, 259, 464, 508
AjnI	CCWGG	4 cut(s)	315, 1272, 1334, 1486
AjuI	GAANNNNNNNTTGG	2 cut(s)	833, 865
AluBI	AGCT	8 cut(s)	151, 313, 523, 564, 743, 755, 1160, 1524
AluI	AGCT	8 cut(s)	151, 313, 523, 564, 743, 755, 1160, 1524
Alw21I	GWGCWC	3 cut(s)	153, 315, 1421
Alw26I	GTCTC	3 cut(s)	334, 351, 396
AlwI	GGATC	3 cut(s)	591, 872, 1264
AlwNI	CAGNNNCTG	1 cut(s)	743
Ama87I	CYCGRG	1 cut(s)	1391
Aor51HI	AGCGCT	1 cut(s)	657
AoxI	GGCC	9 cut(s)	3, 9, 164, 991, 1041, 1290, 1316, 1451, 1484
ApeKI	GCWGC	6 cut(s)	425, 520, 704, 1056, 1119, 1160
ApoI	RAATTY	3 cut(s)	121, 171, 895
ArsI	GACNNNNNNTTYG	2 cut(s)	1130, 1162
AsiGI	ACCGGT	1 cut(s)	884
Asp700I	GAANNNNTTC	1 cut(s)	953
AspA2I	CCTAGG	1 cut(s)	578
AspLEI	GCGC	2 cut(s)	658, 1018
AspS9I	GGNCC	6 cut(s)	164, 590, 992, 1195, 1372, 1451
AsuC2I	CCSGG	5 cut(s)	629, 946, 1112, 1261, 1455
AsuHPI	GGTGA	5 cut(s)	874, 1210, 1216, 1445, 1522
AvaI	CYCGRG	1 cut(s)	1391
AvaII	GGWCC	3 cut(s)	590, 1195, 1372
AvrII	CCTAGG	1 cut(s)	578
BalI	TGGCCA	2 cut(s)	11, 1292
BanI	GGYRCC	1 cut(s)	1130
BanII	GRGCYC	2 cut(s)	153, 315
BbsI	GAAGAC	1 cut(s)	120
Bbv12I	GWGCWC	3 cut(s)	153, 315, 1421
BbvI	GCAGC	6 cut(s)	412, 532, 716, 1068, 1131, 1147
BccI	CCATC	4 cut(s)	704, 854, 903, 1229
BceAI	ACGGC	3 cut(s)	1144, 1303, 1552
BcgI	CGANNNNNNTGC	2 cut(s)	91, 125
BciT130I	CCWGG	4 cut(s)	317, 1274, 1336, 1488
BciVI	GTATCC	1 cut(s)	952
BclI	TGATCA	3 cut(s)	246, 360, 649
BcnI	CCSGG	5 cut(s)	629, 946, 1112, 1261, 1455
BcoDI	GTCTC	3 cut(s)	334, 351, 396
BfaI	CTAG	4 cut(s)	350, 579, 771, 1466
BfmI	CTRYAG	2 cut(s)	763, 1161
BfoI	RGCGCY	2 cut(s)	659, 1019
BfuAI	ACCTGC	2 cut(s)	455, 765
BfuI	GTATCC	1 cut(s)	952
BlnI	CCTAGG	1 cut(s)	578
Bme1390I	CCNGG	9 cut(s)	317, 629, 946, 1112, 1261, 1274, 1336, 1455, 1488
Bme18I	GGWCC	3 cut(s)	590, 1195, 1372
BmeT110I	CYCGRG	1 cut(s)	1391
BmgT120I	GGNCC	6 cut(s)	164, 590, 992, 1195, 1372, 1451
BmiI	GGNNCC	6 cut(s)	165, 469, 592, 1132, 1196, 1197
BmrFI	CCNGG	9 cut(s)	317, 629, 946, 1112, 1261, 1274, 1336, 1455, 1488
BmsI	GCATC	3 cut(s)	1131, 1258, 1498
BoxI	GACNNNNGTC	1 cut(s)	1577
BpiI	GAAGAC	1 cut(s)	120
BplI	GAGNNNNNCTC	2 cut(s)	1374, 1406
BpuEI	CTTGAG	2 cut(s)	473, 1487
BpuMI	CCSGG	5 cut(s)	629, 946, 1112, 1261, 1455
Bsa29I	ATCGAT	1 cut(s)	819
BsaBI	GATNNNNATC	1 cut(s)	365
BsaHI	GRCGYC	1 cut(s)	478
BsaI	GGTCTC	1 cut(s)	351
BsaJI	CCNNGG	7 cut(s)	6, 84, 370, 578, 1006, 1231, 1319
BsaWI	WCCGGW	4 cut(s)	117, 884, 891, 1050
BsaXI	ACNNNNNCTCC	4 cut(s)	314, 344, 1149, 1179
Bse118I	RCCGGY	2 cut(s)	884, 989
Bse1I	ACTGG	1 cut(s)	1036
Bse8I	GATNNNNATC	1 cut(s)	365
BseBI	CCWGG	4 cut(s)	317, 1274, 1336, 1488
BseCI	ATCGAT	1 cut(s)	819
BseDI	CCNNGG	7 cut(s)	6, 84, 370, 578, 1006, 1231, 1319
BseGI	GGATG	6 cut(s)	625, 642, 652, 865, 914, 1513
BseJI	GATNNNNATC	1 cut(s)	365
BseMII	CTCAG	2 cut(s)	422, 1010
BseNI	ACTGG	1 cut(s)	1036
BseRI	GAGGAG	1 cut(s)	564
BseXI	GCAGC	6 cut(s)	412, 532, 716, 1068, 1131, 1147
BseYI	CCCAGC	1 cut(s)	1296
BsgI	GTGCAG	1 cut(s)	1075
Bsh1236I	CGCG	1 cut(s)	1520
Bsh1285I	CGRYCG	1 cut(s)	475
BshFI	GGCC	9 cut(s)	5, 11, 166, 993, 1043, 1292, 1318, 1453, 1486
BshNI	GGYRCC	1 cut(s)	1130
BshTI	ACCGGT	1 cut(s)	884
BshVI	ATCGAT	1 cut(s)	819
BsiEI	CGRYCG	1 cut(s)	475
BsiHKAI	GWGCWC	3 cut(s)	153, 315, 1421
BsiHKCI	CYCGRG	1 cut(s)	1391
BsiWI	CGTACG	1 cut(s)	728
BslFI	GGGAC	2 cut(s)	576, 1181
BsmAI	GTCTC	3 cut(s)	334, 351, 396
BsmBI	CGTCTC	1 cut(s)	334
BsmFI	GGGAC	2 cut(s)	576, 1181
BsmI	GAATGC	1 cut(s)	1121
BsnI	GGCC	9 cut(s)	5, 11, 166, 993, 1043, 1292, 1318, 1453, 1486
Bso31I	GGTCTC	1 cut(s)	351
BsoBI	CYCGRG	1 cut(s)	1391
Bsp1286I	GDGCHC	3 cut(s)	153, 315, 1421
Bsp1407I	TGTACA	1 cut(s)	546
Bsp143I	GATC	9 cut(s)	189, 246, 360, 583, 649, 732, 820, 864, 1269
Bsp19I	CCATGG	1 cut(s)	84
BspANI	GGCC	9 cut(s)	5, 11, 166, 993, 1043, 1292, 1318, 1453, 1486
BspCNI	CTCAG	2 cut(s)	423, 1009
BspDI	ATCGAT	1 cut(s)	819
BspFNI	CGCG	1 cut(s)	1520
BspLI	GGNNCC	6 cut(s)	165, 469, 592, 1132, 1196, 1197
BspMAI	CTGCAG	1 cut(s)	1165
BspMI	ACCTGC	2 cut(s)	455, 765
BspPI	GGATC	3 cut(s)	591, 872, 1264
BspT107I	GGYRCC	1 cut(s)	1130
BspTNI	GGTCTC	1 cut(s)	351
BsrBI	CCGCTC	2 cut(s)	203, 1365
BsrFI	RCCGGY	2 cut(s)	884, 989
BsrGI	TGTACA	1 cut(s)	546
BsrI	ACTGG	1 cut(s)	1036
BssAI	RCCGGY	2 cut(s)	884, 989
BssECI	CCNNGG	7 cut(s)	6, 84, 370, 578, 1006, 1231, 1319
BssMI	GATC	9 cut(s)	189, 246, 360, 583, 649, 732, 820, 864, 1269
BssNI	GRCGYC	1 cut(s)	478
BssT1I	CCWWGG	5 cut(s)	6, 84, 578, 1006, 1231
Bst2UI	CCWGG	4 cut(s)	317, 1274, 1336, 1488
Bst4CI	ACNGT	6 cut(s)	723, 728, 934, 1280, 1342, 1534
BstACI	GRCGYC	1 cut(s)	478
BstAPI	GCANNNNNTGC	1 cut(s)	935
BstAUI	TGTACA	1 cut(s)	546
BstC8I	GCNNGC	7 cut(s)	144, 566, 693, 991, 1316, 1367, 1522
BstDEI	CTNAG	5 cut(s)	206, 419, 431, 996, 1019
BstDSI	CCRYGG	3 cut(s)	84, 370, 1319
BstEII	GGTNACC	4 cut(s)	887, 1067, 1216, 1490
BstF5I	GGATG	6 cut(s)	625, 642, 652, 865, 914, 1513
BstFNI	CGCG	1 cut(s)	1520
BstH2I	RGCGCY	2 cut(s)	659, 1019
BstHHI	GCGC	2 cut(s)	658, 1018
BstKTI	GATC	9 cut(s)	192, 249, 363, 586, 652, 735, 823, 867, 1272
BstMAI	GTCTC	3 cut(s)	334, 351, 396
BstMBI	GATC	9 cut(s)	189, 246, 360, 583, 649, 732, 820, 864, 1269
BstMCI	CGRYCG	1 cut(s)	475
BstMWI	GCNNNNNNNGC	9 cut(s)	148, 517, 692, 701, 935, 1128, 1375, 1466, 1517
BstNI	CCWGG	4 cut(s)	317, 1274, 1336, 1488
BstNSI	RCATGY	2 cut(s)	45, 695
BstPAI	GACNNNNGTC	1 cut(s)	1577
BstPI	GGTNACC	4 cut(s)	887, 1067, 1216, 1490
BstSCI	CCNGG	9 cut(s)	315, 627, 944, 1110, 1259, 1272, 1334, 1453, 1486
BstSFI	CTRYAG	2 cut(s)	763, 1161
BstUI	CGCG	1 cut(s)	1520
BstV1I	GCAGC	6 cut(s)	412, 532, 716, 1068, 1131, 1147
BstV2I	GAAGAC	1 cut(s)	120
Bsu15I	ATCGAT	1 cut(s)	819
BsuI	GTATCC	1 cut(s)	952
BsuRI	GGCC	9 cut(s)	5, 11, 166, 993, 1043, 1292, 1318, 1453, 1486
BsuTUI	ATCGAT	1 cut(s)	819
BtgI	CCRYGG	3 cut(s)	84, 370, 1319
BtsCI	GGATG	6 cut(s)	625, 642, 652, 865, 914, 1513
BtsIMutI	CAGTG	1 cut(s)	102
BveI	ACCTGC	2 cut(s)	455, 765
Cac8I	GCNNGC	7 cut(s)	144, 566, 693, 991, 1316, 1367, 1522
CaiI	CAGNNNCTG	1 cut(s)	743
CfoI	GCGC	2 cut(s)	658, 1018
Cfr10I	RCCGGY	2 cut(s)	884, 989
Cfr13I	GGNCC	6 cut(s)	164, 590, 992, 1195, 1372, 1451
ClaI	ATCGAT	1 cut(s)	819
CseI	GACGC	2 cut(s)	486, 526
Csp6I	GTAC	4 cut(s)	547, 724, 729, 778
CspAI	ACCGGT	1 cut(s)	884
CspCI	CAANNNNNGTGG	2 cut(s)	352, 387
CviAII	CATG	6 cut(s)	42, 85, 587, 692, 901, 1360
CviQI	GTAC	4 cut(s)	547, 724, 729, 778
DdeI	CTNAG	5 cut(s)	206, 419, 431, 996, 1019
DpnI	GATC	9 cut(s)	191, 248, 362, 585, 651, 734, 822, 866, 1271
DpnII	GATC	9 cut(s)	189, 246, 360, 583, 649, 732, 820, 864, 1269
DraIII	CACNNNGTG	1 cut(s)	1425
DrdI	GACNNNNNNGTC	1 cut(s)	326
DseDI	GACNNNNNNGTC	1 cut(s)	326
EaeI	YGGCCR	2 cut(s)	9, 1290
Ecl136II	GAGCTC	2 cut(s)	151, 313
Eco130I	CCWWGG	5 cut(s)	6, 84, 578, 1006, 1231
Eco24I	GRGCYC	2 cut(s)	153, 315
Eco31I	GGTCTC	1 cut(s)	351
Eco32I	GATATC	1 cut(s)	109
Eco47I	GGWCC	3 cut(s)	590, 1195, 1372
Eco47III	AGCGCT	1 cut(s)	657
Eco53kI	GAGCTC	2 cut(s)	151, 313
Eco57I	CTGAAG	1 cut(s)	845
Eco88I	CYCGRG	1 cut(s)	1391
Eco91I	GGTNACC	4 cut(s)	887, 1067, 1216, 1490
EcoICRI	GAGCTC	2 cut(s)	151, 313
EcoO109I	RGGNCCY	2 cut(s)	164, 1195
EcoO65I	GGTNACC	4 cut(s)	887, 1067, 1216, 1490
EcoRI	GAATTC	1 cut(s)	121
EcoRII	CCWGG	4 cut(s)	315, 1272, 1334, 1486
EcoRV	GATATC	1 cut(s)	109
EcoT14I	CCWWGG	5 cut(s)	6, 84, 578, 1006, 1231
EcoT38I	GRGCYC	2 cut(s)	153, 315
ErhI	CCWWGG	5 cut(s)	6, 84, 578, 1006, 1231
Esp3I	CGTCTC	1 cut(s)	334
FaeI	CATG	6 cut(s)	45, 88, 590, 695, 904, 1363
FaqI	GGGAC	2 cut(s)	576, 1181
FatI	CATG	6 cut(s)	41, 84, 586, 691, 900, 1359
FauI	CCCGC	6 cut(s)	1212, 1254, 1358, 1453, 1462, 1585
FbaI	TGATCA	3 cut(s)	246, 360, 649
FblI	GTMKAC	2 cut(s)	1026, 1571
FokI	GGATG	6 cut(s)	632, 649, 659, 872, 921, 1520
FriOI	GRGCYC	2 cut(s)	153, 315
FspBI	CTAG	4 cut(s)	350, 579, 771, 1466
GlaI	GCGC	2 cut(s)	657, 1017
GsaI	CCCAGC	1 cut(s)	1300
HaeII	RGCGCY	2 cut(s)	659, 1019
HaeIII	GGCC	9 cut(s)	5, 11, 166, 993, 1043, 1292, 1318, 1453, 1486
HgaI	GACGC	2 cut(s)	486, 526
HhaI	GCGC	2 cut(s)	658, 1018
Hin1I	GRCGYC	1 cut(s)	478
Hin1II	CATG	6 cut(s)	45, 88, 590, 695, 904, 1363
Hin6I	GCGC	2 cut(s)	656, 1016
HinP1I	GCGC	2 cut(s)	656, 1016
HincII	GTYRAC	3 cut(s)	748, 1027, 1572
HindII	GTYRAC	3 cut(s)	748, 1027, 1572
HinfI	GANTC	5 cut(s)	179, 670, 954, 1023, 1028
HpaI	GTTAAC	1 cut(s)	748
HphI	GGTGA	5 cut(s)	874, 1210, 1216, 1445, 1522
Hpy166II	GTNNAC	8 cut(s)	391, 549, 719, 748, 1027, 1266, 1572, 1587
Hpy188I	TCNGA	5 cut(s)	334, 825, 869, 999, 1033
Hpy188III	TCNNGA	1 cut(s)	176
Hpy8I	GTNNAC	8 cut(s)	391, 549, 719, 748, 1027, 1266, 1572, 1587
Hpy99I	CGWCG	3 cut(s)	480, 919, 1573
HpyAV	CCTTC	6 cut(s)	392, 458, 790, 943, 1054, 1318
HpyCH4III	ACNGT	6 cut(s)	723, 728, 934, 1280, 1342, 1534
HpyCH4IV	ACGT	2 cut(s)	780, 917
HpyF10VI	GCNNNNNNNGC	9 cut(s)	148, 517, 692, 701, 935, 1128, 1375, 1466, 1517
HpyF3I	CTNAG	5 cut(s)	206, 419, 431, 996, 1019
HpySE526I	ACGT	2 cut(s)	780, 917
Hsp92I	GRCGYC	1 cut(s)	478
Hsp92II	CATG	6 cut(s)	45, 88, 590, 695, 904, 1363
HspAI	GCGC	2 cut(s)	656, 1016
KflI	GGGWCCC	1 cut(s)	1195
KroI	GCCGGC	1 cut(s)	989
KroNI	GCCGGC	1 cut(s)	991
Ksp22I	TGATCA	3 cut(s)	246, 360, 649
KspAI	GTTAAC	1 cut(s)	748
Kzo9I	GATC	9 cut(s)	189, 246, 360, 583, 649, 732, 820, 864, 1269
LmnI	GCTCC	5 cut(s)	156, 310, 318, 760, 1157
Lsp1109I	GCAGC	6 cut(s)	412, 532, 716, 1068, 1131, 1147
LweI	GCATC	3 cut(s)	1131, 1258, 1498
MaeI	CTAG	4 cut(s)	350, 579, 771, 1466
MaeII	ACGT	2 cut(s)	780, 917
MalI	GATC	9 cut(s)	191, 248, 362, 585, 651, 734, 822, 866, 1271
MbiI	CCGCTC	2 cut(s)	203, 1365
MboI	GATC	9 cut(s)	189, 246, 360, 583, 649, 732, 820, 864, 1269
MboII	GAAGA	7 cut(s)	125, 805, 838, 841, 844, 1039, 1519
MfeI	CAATTG	2 cut(s)	36, 294
MhlI	GDGCHC	3 cut(s)	153, 315, 1421
MlsI	TGGCCA	2 cut(s)	11, 1292
MluCI	AATT	9 cut(s)	13, 36, 121, 171, 294, 895, 975, 1444, 1603
MluNI	TGGCCA	2 cut(s)	11, 1292
MlyI	GAGTC	2 cut(s)	1022, 1032
MmeI	TCCRAC	1 cut(s)	1056
Mox20I	TGGCCA	2 cut(s)	11, 1292
MroNI	GCCGGC	1 cut(s)	989
MroXI	GAANNNNTTC	1 cut(s)	953
MscI	TGGCCA	2 cut(s)	11, 1292
MseI	TTAA	4 cut(s)	407, 663, 747, 1606
MslI	CAYNNNNRTG	1 cut(s)	1358
Msp20I	TGGCCA	2 cut(s)	11, 1292
MspA1I	CMGCKG	1 cut(s)	1592
MspR9I	CCNGG	9 cut(s)	317, 629, 946, 1112, 1261, 1274, 1336, 1455, 1488
MunI	CAATTG	2 cut(s)	36, 294
Mva1269I	GAATGC	1 cut(s)	1121
MvaI	CCWGG	4 cut(s)	317, 1274, 1336, 1488
MvnI	CGCG	1 cut(s)	1520
MwoI	GCNNNNNNNGC	9 cut(s)	148, 517, 692, 701, 935, 1128, 1375, 1466, 1517
NaeI	GCCGGC	1 cut(s)	991
NciI	CCSGG	5 cut(s)	629, 946, 1112, 1261, 1455
NcoI	CCATGG	1 cut(s)	84
NdeII	GATC	9 cut(s)	189, 246, 360, 583, 649, 732, 820, 864, 1269
NgoMIV	GCCGGC	1 cut(s)	989
NlaIII	CATG	6 cut(s)	45, 88, 590, 695, 904, 1363
NlaIV	GGNNCC	6 cut(s)	165, 469, 592, 1132, 1196, 1197
NmeAIII	GCCGAG	1 cut(s)	172
NmuCI	GTSAC	3 cut(s)	514, 880, 1216
NspI	RCATGY	2 cut(s)	45, 695
PaeI	GCATGC	1 cut(s)	695
PaeR7I	CTCGAG	1 cut(s)	1391
PcsI	WCGNNNNNNNCGW	2 cut(s)	131, 489
PctI	GAATGC	1 cut(s)	1121
PdiI	GCCGGC	1 cut(s)	991
PdmI	GAANNNNTTC	1 cut(s)	953
PfeI	GAWTC	3 cut(s)	179, 670, 954
Pfl23II	CGTACG	1 cut(s)	728
PflMI	CCANNNNNTGG	2 cut(s)	1012, 1386
PfoI	TCCNGGA	4 cut(s)	315, 944, 1272, 1334
PinAI	ACCGGT	1 cut(s)	884
PleI	GAGTC	2 cut(s)	1022, 1031
PpsI	GAGTC	2 cut(s)	1022, 1031
PpuMI	RGGWCCY	1 cut(s)	1195
PshAI	GACNNNNGTC	1 cut(s)	1577
Psp124BI	GAGCTC	2 cut(s)	153, 315
Psp5II	RGGWCCY	1 cut(s)	1195
Psp6I	CCWGG	4 cut(s)	315, 1272, 1334, 1486
PspEI	GGTNACC	4 cut(s)	887, 1067, 1216, 1490
PspFI	CCCAGC	1 cut(s)	1296
PspGI	CCWGG	4 cut(s)	315, 1272, 1334, 1486
PspLI	CGTACG	1 cut(s)	728
PspN4I	GGNNCC	6 cut(s)	165, 469, 592, 1132, 1196, 1197
PspPI	GGNCC	6 cut(s)	164, 590, 992, 1195, 1372, 1451
PspPPI	RGGWCCY	1 cut(s)	1195
PspXI	VCTCGAGB	1 cut(s)	1391
PstI	CTGCAG	1 cut(s)	1165
PstNI	CAGNNNCTG	1 cut(s)	743
RsaI	GTAC	4 cut(s)	548, 725, 730, 779
RsaNI	GTAC	4 cut(s)	547, 724, 729, 778
RseI	CAYNNNNRTG	1 cut(s)	1358
SacI	GAGCTC	2 cut(s)	153, 315
SalI	GTCGAC	2 cut(s)	1025, 1570
SaqAI	TTAA	4 cut(s)	407, 663, 747, 1606
Sau3AI	GATC	9 cut(s)	189, 246, 360, 583, 649, 732, 820, 864, 1269
Sau96I	GGNCC	6 cut(s)	164, 590, 992, 1195, 1372, 1451
SchI	GAGTC	2 cut(s)	1022, 1032
ScrFI	CCNGG	9 cut(s)	317, 629, 946, 1112, 1261, 1274, 1336, 1455, 1488
SduI	GDGCHC	3 cut(s)	153, 315, 1421
SfaNI	GCATC	3 cut(s)	1131, 1258, 1498
SfcI	CTRYAG	2 cut(s)	763, 1161
Sfr274I	CTCGAG	1 cut(s)	1391
SinI	GGWCC	3 cut(s)	590, 1195, 1372
SlaI	CTCGAG	1 cut(s)	1391
SmiMI	CAYNNNNRTG	1 cut(s)	1358
SmlI	CTYRAG	3 cut(s)	452, 1391, 1502
SmoI	CTYRAG	3 cut(s)	452, 1391, 1502
SphI	GCATGC	1 cut(s)	695
Sse9I	AATT	9 cut(s)	13, 36, 121, 171, 294, 895, 975, 1444, 1603
SspI	AATATT	2 cut(s)	844, 1331
SspMI	CTAG	4 cut(s)	350, 579, 771, 1466
SstI	GAGCTC	2 cut(s)	153, 315
StyD4I	CCNGG	9 cut(s)	315, 627, 944, 1110, 1259, 1272, 1334, 1453, 1486
StyI	CCWWGG	5 cut(s)	6, 84, 578, 1006, 1231
TaaI	ACNGT	6 cut(s)	723, 728, 934, 1280, 1342, 1534
TaiI	ACGT	2 cut(s)	783, 920
TaqI	TCGA	8 cut(s)	111, 188, 483, 668, 819, 1026, 1392, 1571
TaqII	GACCGA	1 cut(s)	489
TasI	AATT	9 cut(s)	13, 36, 121, 171, 294, 895, 975, 1444, 1603
TatI	WGTACW	1 cut(s)	546
TauI	GCSGC	5 cut(s)	203, 378, 1514, 1517, 1520
TfiI	GAWTC	3 cut(s)	179, 670, 954
Tru1I	TTAA	4 cut(s)	407, 663, 747, 1606
Tru9I	TTAA	4 cut(s)	407, 663, 747, 1606
TscAI	CASTG	1 cut(s)	109
TseFI	GTSAC	3 cut(s)	514, 880, 1216
TseI	GCWGC	6 cut(s)	425, 520, 704, 1056, 1119, 1160
Tsp45I	GTSAC	3 cut(s)	514, 880, 1216
TspDTI	ATGAA	6 cut(s)	237, 614, 889, 946, 1132, 1364
TspGWI	ACGGA	3 cut(s)	316, 359, 1170
TspRI	CASTG	1 cut(s)	109
Van91I	CCANNNNNTGG	2 cut(s)	1012, 1386
VpaK11BI	GGWCC	3 cut(s)	590, 1195, 1372
XapI	RAATTY	3 cut(s)	121, 171, 895
XceI	RCATGY	2 cut(s)	45, 695
XcmI	CCANNNNNNNNNTGG	1 cut(s)	82
XhoI	CTCGAG	1 cut(s)	1391
XmaJI	CCTAGG	1 cut(s)	578
XmiI	GTMKAC	2 cut(s)	1026, 1571
XmnI	GAANNNNTTC	1 cut(s)	953
XspI	CTAG	4 cut(s)	350, 579, 771, 1466

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.