Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0014881

Physical Location & Seq

Genomic Coordinates Forward (+)

28929822 .. 28932379

Gene Structure 2558 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1210600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGGCCTTAGCCAATTTTCTGCTTCTTCTTCTTTCACTTTCATGTTCTTCTTATGGGGTTACTTCCTACCCTTCCATTCGATCCATGGTTACGCAAACTTGCACTGATGTCGATGACCGAAACTCGTGCCTCACAAACGTGCAAGCCGAGCTCCAAAAAAGGGGGCCTCAAACTCAAGATTCGGTTTCAATCCTAACTGCTGCTCTTACGCATACGCTCAATGAAACAAAAAATGCTATCCAAATCATCACAAAGTTCAACTCTTTGCCCAGAAGTTACAGAGAGAGTGTAGCAATTGAGGACTGCAAGGAGCTCCTAGACTACTCCTTCTCCGAGTTGGCTTGGTCTCTAGGGGAAATGATCAAAATTCGCGGCGGCGACACAAATGTTCACTACGAAGGAAACCTAAAAGCTTGGCTTAGTGCTGCCTTAAGTAACCAAGATACCTGCCTTGAGGGTTTTGAAGGAACTGACCGACGCTTCGAAAGTTTCGTTAGGGGAAGTGTGAAGCAAGTGACACAGCTCATTGGTAATGTTCTTACCTTATATACTCAACTACACAGCTTGCCCTCTAAGCCTCCTAGGCATCATGGTCCCCCAACGAATAAAAGTTCGCCAGGGGATTATTTCCCGGCTTGGATGAGTGAGGAAGATCAAGAGCTGGTTAAATCGAATCCAAAATCGGGTGTGAATCATGCTGTTGTGGCAGCAGATGGGAGTGGACAGTACCGTACGATCACAGAAGCTGTCAATGAAGCTCCAAACTACAGCCCTAGCAGGTACGTTATATATGTGAAGAAGGGAATTTATAGGGAAAACATCGAAATGAAGAAGAACAAAACCAATATTATGTTTGTTGGGGATGGGATTGGACAAACTATCATAACGGGTAACCGGAGTTTCATGCAAGGATGGACTACGTTTAGAACTGCAACAGTCGCCGTATCCGGAAGGGGATTCATAGCAAGGAACCTGACAATTCGCAACACAGCCGCCCCTCAAAACCACCAAGCCGTAGCGCTAAGAGTCGACTCCGACCAGTCGGCCTTCTTCCAGTGCAGCGTGGAGGGTTACCAAGACACCCTCTACGCTCACTCGCTCCGCCAATTCTTCCGTGAGTGCACCATCTACGGCACCGTAGACTTCATTTTTGGCAATGGGGCTGCGGTCCTCCAAAACTGCAAAATCTACACAAGGGTCCCACTCCCACAACAAAAAATCACAATCACTGCCCAAGGTAGAAAAAGCCCACATCAAAGCACAGGGTTTGCAATCCAGGACAGTTTTGTTCTGGCCACCCAGCCGACTTATTTGGGTAGGCCATGGAAGAAATATTCCAGAACTGTTTATATGAACACTTATATGAGTAGGTTGGTCCAGCCCAGAGGGTGGCTTGAGTGGAATGGGGATTTTGCTTTGGGCACGTTGTGGTATGGTGAGTATAAGAATTATGGGCCGGGTGCAGCTATGTCGGGTCGGGTCAAATGGCGTGGTTACCATATACTCAGGGATGCTGCTGCGGCGAGCTTCTTCACCGTGGGGAGGTTTATTGATGGGATGGCTTGGTTGCCGTCGACCGGTATCAAGTTTACAGCAGGGTTTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

536

Amino Acids

59.72

Weight (kDa)

9.11

Isoelectric Point (pI)

40.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	29 - 182	4.9e-25	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	233 - 519	3e-137	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014881)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05620
fragaria_vesca	FvH4_6g06871
malus_domestica	MD04G1198000.v1.1 MD12G1210600.v1.1
prunus_persica	Prupe.6G318500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0456351
rosa_laevigata	RLG00000025310
rosa_multiflora	Rmu_sc0002607.1_g000020 Rmu_sc0018054.1_g000002
rosa_roxburghii	Rroxscaffold_6G00422790
rosa_rugosa	Rorug03G0012700
rosa_samantha	Rh3AG072800 Rh3BG074900 Rh3CG074500 Rh3DG076300
rosa_wichuraiana	Rw3G006250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	455, 768
AccB1I	GGYRCC	1 cut(s)	1133
AccB7I	CCANNNNNTGG	1 cut(s)	1389
AccI	GTMKAC	3 cut(s)	1029, 1140, 1574
AccII	CGCG	1 cut(s)	372
AccIII	TCCGGA	1 cut(s)	947
AciI	CCGC	6 cut(s)	372, 375, 993, 1102, 1166, 1520
AclWI	GGATC	1 cut(s)	75
AcoI	YGGCCR	1 cut(s)	1293
AcsI	RAATTY	2 cut(s)	366, 804
AdeI	CACNNNGTG	1 cut(s)	1428
AfaI	GTAC	3 cut(s)	728, 733, 782
AfeI	AGCGCT	1 cut(s)	1020
AfiI	CCNNNNNNNGG	5 cut(s)	160, 683, 1389, 1542, 1577
AflII	CTTAAG	1 cut(s)	430
AgeI	ACCGGT	1 cut(s)	1577
AgsI	TTSAA	3 cut(s)	189, 259, 464
AjnI	CCWGG	2 cut(s)	616, 1275
AleI	CACNNNNGTG	1 cut(s)	137
Alw21I	GWGCWC	3 cut(s)	153, 315, 1124
Alw26I	GTCTC	1 cut(s)	351
Alw44I	GTGCAC	1 cut(s)	1120
AlwI	GGATC	1 cut(s)	75
AlwNI	CAGNNNCTG	1 cut(s)	746
Aor13HI	TCCGGA	1 cut(s)	947
Aor51HI	AGCGCT	1 cut(s)	1020
AoxI	GGCC	6 cut(s)	3, 164, 1044, 1293, 1319, 1454
ApaLI	GTGCAC	1 cut(s)	1120
ApeKI	GCWGC	8 cut(s)	200, 425, 707, 1059, 1163, 1463, 1514, 1517
ApoI	RAATTY	2 cut(s)	366, 804
ArsI	GACNNNNNNTTYG	4 cut(s)	464, 496, 1133, 1165
AsiGI	ACCGGT	1 cut(s)	1577
AspA2I	CCTAGG	1 cut(s)	581
AspLEI	GCGC	1 cut(s)	1021
AspS9I	GGNCC	6 cut(s)	164, 593, 1168, 1198, 1375, 1454
AsuC2I	CCSGG	2 cut(s)	632, 1458
AsuHPI	GGTGA	2 cut(s)	1448, 1525
AsuII	TTCGAA	1 cut(s)	483
AvaII	GGWCC	4 cut(s)	593, 1168, 1198, 1375
AvrII	CCTAGG	1 cut(s)	581
BaeGI	GKGCMC	2 cut(s)	1124, 1424
BalI	TGGCCA	1 cut(s)	1295
BanI	GGYRCC	1 cut(s)	1133
BanII	GRGCYC	2 cut(s)	153, 315
BauI	CACGAG	1 cut(s)	124
Bbv12I	GWGCWC	3 cut(s)	153, 315, 1124
BbvI	GCAGC	8 cut(s)	187, 412, 719, 1071, 1150, 1475, 1501, 1504
BccI	CCATC	6 cut(s)	707, 857, 906, 1133, 1547, 1552
BceAI	ACGGC	4 cut(s)	926, 998, 1147, 1555
BcgI	CGANNNNNNTGC	6 cut(s)	91, 108, 125, 142, 1452, 1486
BciT130I	CCWGG	2 cut(s)	618, 1277
BciVI	GTATCC	1 cut(s)	955
BclI	TGATCA	1 cut(s)	360
BcnI	CCSGG	2 cut(s)	632, 1458
BcoDI	GTCTC	1 cut(s)	351
BfaI	CTAG	4 cut(s)	317, 350, 582, 774
BfmI	CTRYAG	1 cut(s)	766
BfoI	RGCGCY	1 cut(s)	1022
BfrI	CTTAAG	1 cut(s)	430
BfuAI	ACCTGC	2 cut(s)	455, 768
BfuI	GTATCC	1 cut(s)	955
BglI	GCCNNNNNGGC	1 cut(s)	583
BlnI	CCTAGG	1 cut(s)	581
Bme1390I	CCNGG	4 cut(s)	618, 632, 1277, 1458
Bme18I	GGWCC	4 cut(s)	593, 1168, 1198, 1375
BmgT120I	GGNCC	6 cut(s)	164, 593, 1168, 1198, 1375, 1454
BmiI	GGNNCC	6 cut(s)	165, 595, 971, 1135, 1199, 1200
BmrFI	CCNGG	4 cut(s)	618, 632, 1277, 1458
BmsI	GCATC	2 cut(s)	595, 1501
Bpu10I	CCTNAGC	1 cut(s)	7
Bpu14I	TTCGAA	1 cut(s)	483
BpuEI	CTTGAG	3 cut(s)	159, 473, 1415
BpuMI	CCSGG	2 cut(s)	632, 1458
BsaI	GGTCTC	1 cut(s)	351
BsaJI	CCNNGG	6 cut(s)	84, 581, 617, 1234, 1322, 1536
BsaWI	WCCGGW	3 cut(s)	894, 947, 1577
BsaXI	ACNNNNNCTCC	2 cut(s)	314, 344
Bsc4I	CCNNNNNNNGG	5 cut(s)	160, 683, 1389, 1542, 1577
Bse118I	RCCGGY	1 cut(s)	1577
Bse1I	ACTGG	2 cut(s)	1039, 1054
Bse3DI	GCAATG	1 cut(s)	1162
BseAI	TCCGGA	1 cut(s)	947
BseBI	CCWGG	2 cut(s)	618, 1277
BseDI	CCNNGG	6 cut(s)	84, 581, 617, 1234, 1322, 1536
BseGI	GGATG	5 cut(s)	645, 868, 917, 1516, 1563
BseLI	CCNNNNNNNGG	5 cut(s)	160, 683, 1389, 1542, 1577
BseMI	GCAATG	1 cut(s)	1162
BseMII	CTCAG	1 cut(s)	1519
BseNI	ACTGG	2 cut(s)	1039, 1054
BseSI	GKGCMC	2 cut(s)	1124, 1424
BseXI	GCAGC	8 cut(s)	187, 412, 719, 1071, 1150, 1475, 1501, 1504
BseYI	CCCAGC	1 cut(s)	1299
BsgI	GTGCAG	2 cut(s)	1078, 1482
Bsh1236I	CGCG	1 cut(s)	372
Bsh1285I	CGRYCG	1 cut(s)	1578
BshFI	GGCC	6 cut(s)	5, 166, 1046, 1295, 1321, 1456
BshNI	GGYRCC	1 cut(s)	1133
BshTI	ACCGGT	1 cut(s)	1577
BsiEI	CGRYCG	1 cut(s)	1578
BsiHKAI	GWGCWC	3 cut(s)	153, 315, 1124
BsiSI	CCGG	5 cut(s)	632, 895, 948, 1457, 1578
BsiWI	CGTACG	1 cut(s)	731
BslFI	GGGAC	2 cut(s)	579, 1184
BslI	CCNNNNNNNGG	5 cut(s)	160, 683, 1389, 1542, 1577
BsmAI	GTCTC	1 cut(s)	351
BsmFI	GGGAC	2 cut(s)	579, 1184
BsnI	GGCC	6 cut(s)	5, 166, 1046, 1295, 1321, 1456
Bso31I	GGTCTC	1 cut(s)	351
Bsp119I	TTCGAA	1 cut(s)	483
Bsp1286I	GDGCHC	4 cut(s)	153, 315, 1124, 1424
Bsp13I	TCCGGA	1 cut(s)	947
Bsp143I	GATC	4 cut(s)	80, 360, 652, 735
Bsp19I	CCATGG	2 cut(s)	84, 1322
BspACI	CCGC	6 cut(s)	372, 375, 993, 1102, 1166, 1520
BspANI	GGCC	6 cut(s)	5, 166, 1046, 1295, 1321, 1456
BspCNI	CTCAG	1 cut(s)	1518
BspEI	TCCGGA	1 cut(s)	947
BspFNI	CGCG	1 cut(s)	372
BspLI	GGNNCC	6 cut(s)	165, 595, 971, 1135, 1199, 1200
BspMI	ACCTGC	2 cut(s)	455, 768
BspPI	GGATC	1 cut(s)	75
BspT104I	TTCGAA	1 cut(s)	483
BspT107I	GGYRCC	1 cut(s)	1133
BspTI	CTTAAG	1 cut(s)	430
BspTNI	GGTCTC	1 cut(s)	351
BsrDI	GCAATG	1 cut(s)	1162
BsrFI	RCCGGY	1 cut(s)	1577
BsrI	ACTGG	2 cut(s)	1039, 1054
BssAI	RCCGGY	1 cut(s)	1577
BssECI	CCNNGG	6 cut(s)	84, 581, 617, 1234, 1322, 1536
BssMI	GATC	4 cut(s)	80, 360, 652, 735
BssSI	CACGAG	1 cut(s)	124
BssT1I	CCWWGG	4 cut(s)	84, 581, 1234, 1322
Bst2BI	CACGAG	1 cut(s)	124
Bst2UI	CCWGG	2 cut(s)	618, 1277
Bst4CI	ACNGT	7 cut(s)	726, 731, 937, 1138, 1283, 1345, 1537
BstAFI	CTTAAG	1 cut(s)	430
BstBI	TTCGAA	1 cut(s)	483
BstC8I	GCNNGC	3 cut(s)	144, 566, 1525
BstDEI	CTNAG	5 cut(s)	7, 419, 573, 1022, 1505
BstDSI	CCRYGG	3 cut(s)	84, 1322, 1536
BstEII	GGTNACC	3 cut(s)	890, 1070, 1493
BstF5I	GGATG	5 cut(s)	645, 868, 917, 1516, 1563
BstFNI	CGCG	1 cut(s)	372
BstH2I	RGCGCY	1 cut(s)	1022
BstHHI	GCGC	1 cut(s)	1021
BstKTI	GATC	4 cut(s)	83, 363, 655, 738
BstMAI	GTCTC	1 cut(s)	351
BstMBI	GATC	4 cut(s)	80, 360, 652, 735
BstMCI	CGRYCG	1 cut(s)	1578
BstMWI	GCNNNNNNNGC	6 cut(s)	148, 574, 583, 704, 938, 1520
BstNI	CCWGG	2 cut(s)	618, 1277
BstPI	GGTNACC	3 cut(s)	890, 1070, 1493
BstSCI	CCNGG	4 cut(s)	616, 630, 1275, 1456
BstSFI	CTRYAG	1 cut(s)	766
BstSLI	GKGCMC	2 cut(s)	1124, 1424
BstUI	CGCG	1 cut(s)	372
BstV1I	GCAGC	8 cut(s)	187, 412, 719, 1071, 1150, 1475, 1501, 1504
BsuI	GTATCC	1 cut(s)	955
BsuRI	GGCC	6 cut(s)	5, 166, 1046, 1295, 1321, 1456
BtgI	CCRYGG	3 cut(s)	84, 1322, 1536
BtsCI	GGATG	5 cut(s)	645, 868, 917, 1516, 1563
BtsI	GCAGTG	1 cut(s)	1227
BtsIMutI	CAGTG	3 cut(s)	102, 1061, 1227
BveI	ACCTGC	2 cut(s)	455, 768
Cac8I	GCNNGC	3 cut(s)	144, 566, 1525
CaiI	CAGNNNCTG	1 cut(s)	746
CfoI	GCGC	1 cut(s)	1021
Cfr10I	RCCGGY	1 cut(s)	1577
Cfr13I	GGNCC	6 cut(s)	164, 593, 1168, 1198, 1375, 1454
CseI	GACGC	1 cut(s)	486
Csp6I	GTAC	3 cut(s)	727, 732, 781
CspAI	ACCGGT	1 cut(s)	1577
CspCI	CAANNNNNGTGG	2 cut(s)	1471, 1506
CviAII	CATG	6 cut(s)	42, 85, 590, 695, 904, 1323
CviQI	GTAC	3 cut(s)	727, 732, 781
DdeI	CTNAG	5 cut(s)	7, 419, 573, 1022, 1505
DpnI	GATC	4 cut(s)	82, 362, 654, 737
DpnII	GATC	4 cut(s)	80, 360, 652, 735
DraIII	CACNNNGTG	1 cut(s)	1428
EaeI	YGGCCR	1 cut(s)	1293
EciI	GGCGGA	1 cut(s)	1091
Ecl136II	GAGCTC	2 cut(s)	151, 313
Eco130I	CCWWGG	4 cut(s)	84, 581, 1234, 1322
Eco24I	GRGCYC	2 cut(s)	153, 315
Eco31I	GGTCTC	1 cut(s)	351
Eco47I	GGWCC	4 cut(s)	593, 1168, 1198, 1375
Eco47III	AGCGCT	1 cut(s)	1020
Eco53kI	GAGCTC	2 cut(s)	151, 313
Eco91I	GGTNACC	3 cut(s)	890, 1070, 1493
EcoICRI	GAGCTC	2 cut(s)	151, 313
EcoO109I	RGGNCCY	2 cut(s)	164, 1198
EcoO65I	GGTNACC	3 cut(s)	890, 1070, 1493
EcoRII	CCWGG	2 cut(s)	616, 1275
EcoT14I	CCWWGG	4 cut(s)	84, 581, 1234, 1322
EcoT38I	GRGCYC	2 cut(s)	153, 315
ErhI	CCWWGG	4 cut(s)	84, 581, 1234, 1322
FaeI	CATG	6 cut(s)	45, 88, 593, 698, 907, 1326
FalI	AAGNNNNNCTT	2 cut(s)	402, 434
FaqI	GGGAC	2 cut(s)	579, 1184
FatI	CATG	6 cut(s)	41, 84, 589, 694, 903, 1322
FbaI	TGATCA	1 cut(s)	360
FblI	GTMKAC	3 cut(s)	1029, 1140, 1574
FokI	GGATG	5 cut(s)	652, 875, 924, 1523, 1570
FriOI	GRGCYC	2 cut(s)	153, 315
FspBI	CTAG	4 cut(s)	317, 350, 582, 774
GlaI	GCGC	1 cut(s)	1020
GsaI	CCCAGC	1 cut(s)	1303
HaeII	RGCGCY	1 cut(s)	1022
HaeIII	GGCC	6 cut(s)	5, 166, 1046, 1295, 1321, 1456
HapII	CCGG	5 cut(s)	632, 895, 948, 1457, 1578
HgaI	GACGC	1 cut(s)	486
HhaI	GCGC	1 cut(s)	1021
Hin1II	CATG	6 cut(s)	45, 88, 593, 698, 907, 1326
Hin6I	GCGC	1 cut(s)	1019
HinP1I	GCGC	1 cut(s)	1019
HincII	GTYRAC	2 cut(s)	1030, 1575
HindII	GTYRAC	2 cut(s)	1030, 1575
HindIII	AAGCTT	1 cut(s)	411
HinfI	GANTC	6 cut(s)	179, 673, 691, 957, 1026, 1031
HpaII	CCGG	5 cut(s)	632, 895, 948, 1457, 1578
HphI	GGTGA	2 cut(s)	1448, 1525
Hpy166II	GTNNAC	7 cut(s)	391, 722, 1030, 1122, 1141, 1575, 1590
Hpy188I	TCNGA	2 cut(s)	334, 1036
Hpy188III	TCNNGA	4 cut(s)	176, 656, 948, 1338
Hpy8I	GTNNAC	7 cut(s)	391, 722, 1030, 1122, 1141, 1575, 1590
Hpy99I	CGWCG	2 cut(s)	480, 1576
HpyAV	CCTTC	7 cut(s)	81, 337, 392, 458, 793, 945, 1057
HpyCH4III	ACNGT	7 cut(s)	726, 731, 937, 1138, 1283, 1345, 1537
HpyCH4IV	ACGT	4 cut(s)	138, 783, 920, 1424
HpyF10VI	GCNNNNNNNGC	6 cut(s)	148, 574, 583, 704, 938, 1520
HpyF3I	CTNAG	5 cut(s)	7, 419, 573, 1022, 1505
HpySE526I	ACGT	4 cut(s)	138, 783, 920, 1424
Hsp92II	CATG	6 cut(s)	45, 88, 593, 698, 907, 1326
HspAI	GCGC	1 cut(s)	1019
KflI	GGGWCCC	1 cut(s)	1198
Kpn2I	TCCGGA	1 cut(s)	947
Ksp22I	TGATCA	1 cut(s)	360
Kzo9I	GATC	4 cut(s)	80, 360, 652, 735
LmnI	GCTCC	5 cut(s)	156, 310, 318, 763, 1104
Lsp1109I	GCAGC	8 cut(s)	187, 412, 719, 1071, 1150, 1475, 1501, 1504
LweI	GCATC	2 cut(s)	595, 1501
MaeI	CTAG	4 cut(s)	317, 350, 582, 774
MaeII	ACGT	4 cut(s)	138, 783, 920, 1424
MaeIII	GTNAC	8 cut(s)	58, 88, 275, 434, 514, 890, 1070, 1493
MalI	GATC	4 cut(s)	82, 362, 654, 737
MboI	GATC	4 cut(s)	80, 360, 652, 735
MfeI	CAATTG	1 cut(s)	294
MhlI	GDGCHC	4 cut(s)	153, 315, 1124, 1424
MlsI	TGGCCA	1 cut(s)	1295
MluCI	AATT	7 cut(s)	13, 294, 366, 804, 978, 1106, 1447
MluNI	TGGCCA	1 cut(s)	1295
MlyI	GAGTC	2 cut(s)	1025, 1035
MmeI	TCCRAC	1 cut(s)	1059
Mox20I	TGGCCA	1 cut(s)	1295
MroI	TCCGGA	1 cut(s)	947
MscI	TGGCCA	1 cut(s)	1295
MseI	TTAA	2 cut(s)	431, 666
MslI	CAYNNNNRTG	4 cut(s)	40, 137, 824, 1361
Msp20I	TGGCCA	1 cut(s)	1295
MspCI	CTTAAG	1 cut(s)	430
MspI	CCGG	5 cut(s)	632, 895, 948, 1457, 1578
MspR9I	CCNGG	4 cut(s)	618, 632, 1277, 1458
MunI	CAATTG	1 cut(s)	294
MvaI	CCWGG	2 cut(s)	618, 1277
MvnI	CGCG	1 cut(s)	372
MwoI	GCNNNNNNNGC	6 cut(s)	148, 574, 583, 704, 938, 1520
NciI	CCSGG	2 cut(s)	632, 1458
NcoI	CCATGG	2 cut(s)	84, 1322
NdeII	GATC	4 cut(s)	80, 360, 652, 735
NlaIII	CATG	6 cut(s)	45, 88, 593, 698, 907, 1326
NlaIV	GGNNCC	6 cut(s)	165, 595, 971, 1135, 1199, 1200
NmeAIII	GCCGAG	1 cut(s)	172
NmuCI	GTSAC	1 cut(s)	514
NspV	TTCGAA	1 cut(s)	483
OliI	CACNNNNGTG	1 cut(s)	137
PcsI	WCGNNNNNNNCGW	2 cut(s)	144, 489
PfeI	GAWTC	4 cut(s)	179, 673, 691, 957
Pfl23II	CGTACG	1 cut(s)	731
PflMI	CCANNNNNTGG	1 cut(s)	1389
PfoI	TCCNGGA	1 cut(s)	1275
PinAI	ACCGGT	1 cut(s)	1577
PleI	GAGTC	2 cut(s)	1025, 1034
PpsI	GAGTC	2 cut(s)	1025, 1034
PpuMI	RGGWCCY	1 cut(s)	1198
Psp124BI	GAGCTC	2 cut(s)	153, 315
Psp5II	RGGWCCY	1 cut(s)	1198
Psp6I	CCWGG	2 cut(s)	616, 1275
PspEI	GGTNACC	3 cut(s)	890, 1070, 1493
PspFI	CCCAGC	1 cut(s)	1299
PspGI	CCWGG	2 cut(s)	616, 1275
PspLI	CGTACG	1 cut(s)	731
PspN4I	GGNNCC	6 cut(s)	165, 595, 971, 1135, 1199, 1200
PspPI	GGNCC	6 cut(s)	164, 593, 1168, 1198, 1375, 1454
PspPPI	RGGWCCY	1 cut(s)	1198
PstNI	CAGNNNCTG	1 cut(s)	746
RsaI	GTAC	3 cut(s)	728, 733, 782
RsaNI	GTAC	3 cut(s)	727, 732, 781
RseI	CAYNNNNRTG	4 cut(s)	40, 137, 824, 1361
SacI	GAGCTC	2 cut(s)	153, 315
SalI	GTCGAC	2 cut(s)	1028, 1573
SaqAI	TTAA	2 cut(s)	431, 666
Sau3AI	GATC	4 cut(s)	80, 360, 652, 735
Sau96I	GGNCC	6 cut(s)	164, 593, 1168, 1198, 1375, 1454
SchI	GAGTC	2 cut(s)	1025, 1035
ScrFI	CCNGG	4 cut(s)	618, 632, 1277, 1458
SduI	GDGCHC	4 cut(s)	153, 315, 1124, 1424
SfaNI	GCATC	2 cut(s)	595, 1501
SfcI	CTRYAG	1 cut(s)	766
SfuI	TTCGAA	1 cut(s)	483
SinI	GGWCC	4 cut(s)	593, 1168, 1198, 1375
SmiMI	CAYNNNNRTG	4 cut(s)	40, 137, 824, 1361
SmlI	CTYRAG	4 cut(s)	174, 430, 452, 1394
SmoI	CTYRAG	4 cut(s)	174, 430, 452, 1394
Sse9I	AATT	7 cut(s)	13, 294, 366, 804, 978, 1106, 1447
SsiI	CCGC	6 cut(s)	372, 375, 993, 1102, 1166, 1520
SspI	AATATT	2 cut(s)	847, 1334
SspMI	CTAG	4 cut(s)	317, 350, 582, 774
SstI	GAGCTC	2 cut(s)	153, 315
StyD4I	CCNGG	4 cut(s)	616, 630, 1275, 1456
StyI	CCWWGG	4 cut(s)	84, 581, 1234, 1322
TaaI	ACNGT	7 cut(s)	726, 731, 937, 1138, 1283, 1345, 1537
TaiI	ACGT	4 cut(s)	141, 786, 923, 1427
TaqI	TCGA	7 cut(s)	79, 111, 483, 671, 822, 1029, 1574
TaqII	GACCGA	2 cut(s)	132, 489
TasI	AATT	7 cut(s)	13, 294, 366, 804, 978, 1106, 1447
TauI	GCSGC	4 cut(s)	375, 378, 995, 1523
TfiI	GAWTC	4 cut(s)	179, 673, 691, 957
Tru1I	TTAA	2 cut(s)	431, 666
Tru9I	TTAA	2 cut(s)	431, 666
TscAI	CASTG	3 cut(s)	109, 1061, 1234
TseFI	GTSAC	1 cut(s)	514
TseI	GCWGC	8 cut(s)	200, 425, 707, 1059, 1163, 1463, 1514, 1517
Tsp45I	GTSAC	1 cut(s)	514
TspDTI	ATGAA	8 cut(s)	30, 237, 768, 842, 892, 949, 1135, 1367
TspGWI	ACGGA	1 cut(s)	1103
TspRI	CASTG	3 cut(s)	109, 1061, 1234
Van91I	CCANNNNNTGG	1 cut(s)	1389
Vha464I	CTTAAG	1 cut(s)	430
VneI	GTGCAC	1 cut(s)	1120
VpaK11BI	GGWCC	4 cut(s)	593, 1168, 1198, 1375
XapI	RAATTY	2 cut(s)	366, 804
XcmI	CCANNNNNNNNNTGG	1 cut(s)	82
XmaJI	CCTAGG	1 cut(s)	581
XmiI	GTMKAC	3 cut(s)	1029, 1140, 1574
XspI	CTAG	4 cut(s)	317, 350, 582, 774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.